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Congming Wu, Yingchao Wang, Xiaomin Shi, Shuang Wang, Hongwei Ren, Zhangqi Shen, Yang Wang, Juchun Lin, Shaolin Wang
Extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) strains are emerging around the world as a source of resistance to β-lactam antibiotics such as ampicillin, cefotaxime, and ceftazidime. mcr-1 is a novel plasmid-mediated gene conferring resistance to colistin. The aim of this study was to investigate the prevalence of ESBL-EC mcr-1 of chicken origin in the different provinces of China during 2008-2014. Overall, 341 of 821 isolates were determined to be ESBL-EC strains, and the proportion of ESBL-positive strains almost doubled from 2008 to 2014...
March 14, 2018: Emerging Microbes & Infections
Giulia Niero, Valeria Bortolaia, Michele Vanni, Luigi Intorre, Luca Guardabassi, Alessandra Piccirillo
The aim was to investigate occurrence and diversity of plasmid-mediated resistance to third-generation cephalosporins (3GC) and quinolones in clinical Escherichia coli from 200 industrial poultry farms across Italy. E. coli was isolated from colibacillosis lesions in turkeys (n = 109), broilers (n = 98) and layers (n = 22) between 2008 and 2012. 3GC-resistant isolates were screened for extended-spectrum and AmpC β-lactamase (ESBL/AmpC), while all isolates were tested for plasmid-mediated quinolone resistance (PMQR) genes...
March 2018: Veterinary Microbiology
Anna Lena Zogg, Katrin Zurfluh, Sarah Schmitt, Magdalena Nüesch-Inderbinen, Roger Stephan
Among 64 uropathogenic Escherichia coli (UPEC) isolated from 13 cats and 51 dogs, 35 were extended-spectrum beta-lactamase (ESBL) producers, and 29 were non-ESBL producers. Forty-six (71.9%) of the isolates were multidrug resistant (MDR). Among the ESBL producers, blaCTX-M-15 (n = 17/48.6% of the blaESBLs ), blaCTX-M-1 (n = 10/28.6%), blaCTX-M-55 (n = 4/11.4%), blaCTX-M-14 (n = 3/8.6%), and blaCTX-M-27 (n = 1/2.9%) were identified. The plasmid-mediated fluoroquinolone resistance genes aac(6')-Ib-cr, qnrB and the azithromycin resistance gene mph(A) were detected in 17 (26...
March 2018: Veterinary Microbiology
Sandrine Baron, Laetitia Le Devendec, Fabrice Touzain, Eric Jouy, Pierrick Lucas, Claire de Boisséson, Emeline Larvor, Isabelle Kempf
Resistance to extended-spectrum cephalosporins (ESCs) is mostly borne by conjugative plasmids. The aim of the present study was to evaluate the characteristics and diversity of ESC resistance plasmids in Escherichia coli from different free-range broiler flocks in France, and their persistence in flocks during rearing. Two hatcheries were selected. Faecal samples from 11 flocks were collected from before their arrival on the broiler production farm up to their slaughter at the end of the rearing period. A selection of 25 E...
March 2018: Veterinary Microbiology
Yoshihiko Maeyama, Yui Taniguchi, Wataru Hayashi, Yusuke Ohsaki, Shunsuke Osaka, Shota Koide, Kiyoko Tamai, Yukiko Nagano, Yoshichika Arakawa, Noriyuki Nagano
In recent years, besides the widespread occurrence of extended-spectrum β-lactamase (ESBL)- and/or plasmid-mediated AmpC (pAmpC)-producing Enterobacteriaceae in both healthcare and community settings of humans, the third-generation cephalosporin (3GC)-resistant microbes have also been reported from companion animals worldwide. Here, we characterized ESBL- and/or pAmpC-producing Enterobacteriaceae clinical isolates from companion animals. Among the 487 clinical isolates mainly from urine of dogs and cats between May and September 2016, 104 non-repetitive isolates were resistant to the 3GC, and they consisted of 81 of 381 (21...
March 2018: Veterinary Microbiology
Cliodna A M McNulty, Donna M Lecky, Li Xu-McCrae, Deborah Nakiboneka-Ssenabulya, Keun-Taik Chung, Tom Nichols, Helen Lucy Thomas, Mike Thomas, Adela Alvarez-Buylla, Kim Turner, Sahida Shabir, Susan Manzoor, Stephen Smith, Linda Crocker, Peter M Hawkey
Background: ESBL-producing Enterobacteriaceae (ESBLPE) are increasing in prevalence worldwide and are more difficult to treat than non-ESBLPE. Their prevalence in the UK general population is unknown, as the only previous UK ESBLPE faecal colonization study involved patients with diarrhoea. Objectives: To estimate the prevalence of CTX-M ESBLPE faecal colonization in the general adult population of England in 2014, and investigate risk factors. Methods: A stratified random sample of 58 337 registered patients from 16 general practices within four areas of England were invited to participate by returning faeces specimens and self-completed questionnaires...
March 5, 2018: Journal of Antimicrobial Chemotherapy
Qiuxia Ma, Yong Huang, Jian Wang, Xuebin Xu, Jane Hawkey, Chaojie Yang, Beibei Liang, Xiaofeng Hu, Fuli Wu, Xiaoxia Yang, Jinyan Wang, Rongrui Li, Peng Li, Jing Xie, Leili Jia, Ligui Wang, Rongzhang Hao, Yigang Tong, Kathryn E Holt, Shaofu Qiu, Yansong Sun, Hongbin Song
Since the plasmid-mediated polymyxin resistance gene mcr-1 was first reported in Escherichia coli and Klebsiella pneumoniae in China, only one mcr-1-positive isolate of Shigella sonnei, containing inactivated mcr-1, has been reported worldwide. Here, we screened 1650 historical S. sonnei strains isolated from 2003 to 2015 in China for mcr-1. We determined the antimicrobial susceptibilities and resistance genes of mcr-1-positive isolates and investigated the transferability of polymyxin resistance by plasmid conjugation...
February 28, 2018: International Journal of Antimicrobial Agents
Tiago Casella, Andressa Batista Zequini de Morais, Diego Diniz de Paula Barcelos, Fernanda Modesto Tolentino, Louise Teixeira Cerdeira, Maria Fernanda Campagnari Bueno, Gabriela Rodrigues Francisco, Leonardo Neves de Andrade, Ana Lucia da Costa Darini, Doroti de Oliveira Garcia, Nilton Lincopan, Mara Corrêa Lelles Nogueira
OBJECTIVES: Klebsiella pneumoniae is considered an opportunistic pathogen and important agent of nosocomial and community infections. It presents the ability to capture and harbor several antimicrobial resistance genes, and in this context, the extensive use of carbapenems to treat serious infections was responsible for selection of several resistance genes. In this study, we report the draft genome sequence of a KPC-2-procuding K. pneumoniae strain (Kp10) simultaneously harboring the blaCTX-M-15 and blaCTX-M-59 genes, isolated from urine culture of a patient with Parkinson's disease...
February 21, 2018: Journal of Global Antimicrobial Resistance
Nuno F F Moreira, Carlos Narciso-da-Rocha, M Inmaculada Polo-López, Luisa M Pastrana-Martínez, Joaquim L Faria, Célia M Manaia, Pilar Fernández-Ibáñez, Olga C Nunes, Adrián M T Silva
Solar-driven advanced oxidation processes were studied in a pilot-scale photoreactor, as tertiary treatments of effluents from an urban wastewater treatment plant. Solar-H2 O2 , heterogeneous photocatalysis (with and/or without the addition of H2 O2 and employing three different photocatalysts) and the photo-Fenton process were investigated. Chemical (sulfamethoxazole, carbamazepine, and diclofenac) and biological contaminants (faecal contamination indicators, their antibiotic resistant counterparts, 16S rRNA and antibiotic resistance genes), as well as the whole bacterial community, were characterized...
February 3, 2018: Water Research
Aurora Piazza, Francesco Comandatore, Francesca Romeri, Cristina Pagani, Anna Maria Floriano, Annalisa Ridolfo, Carlo Antona, Matteo Brilli, Vittoria Mattioni Marchetti, Claudio Bandi, Maria Rita Gismondo, Sara Giordana Rimoldi
We investigated an Italian OXA-181-producing Escherichia coli clinical isolate (ECS1_14) by whole-genome sequencing. The strain coharbored blaCTX-M-15 , blaCMY-2 , and qnrS1 genes; it belonged to ST410(Achtman)/ST692(Pasteur) and phylogroup A. The blaOXA-181 gene was harbored on a plasmid highly similar (99% identity) to the pOXA181_EC14828 plasmid, recently reported in China.
February 23, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Dongdong Zhao, Zhihui Zhou, Xiaoting Hua, Huichuan Zhang, Jingjing Quan, Xi Li, Yunsong Yu
Here we report the first clinical Escherichia coli isolate co-harboring mcr-1, blaKPC-2 and two copies of fosA3 from China. The five plasmids of the isolate were completely sequenced and analyzed. Gene mcr-1 and blaKPC-2 were located on IncI2 and IncR plasmid, respectively. A variety of other resistance determinants such as fosA3 (two copies), blaCTX-M-123 , blaOXA-1 and blaCTX-M-65 were also identified from the rest plasmids.
February 19, 2018: Infection, Genetics and Evolution
Tolbert Sonda, Happiness Kumburu, Marco van Zwetselaar, Michael Alifrangis, Blandina T Mmbaga, Ole Lund, Frank M Aarestrup, Gibson Kibiki
Emergence and spread of extended spectrum beta-lactamase (ESBL)-producing gram-negative bacteria, mainly due to CTX-M, is a major global public health problem. Patients infected with ESBL-producing gram-negative bacteria have an increased risk of treatment failure and death. We investigated the prevalence and risk factors for CTX-M gram-negative bacteria isolated from clinical specimens of patients hospitalized at a tertiary care hospital in Kilimanjaro, Tanzania. Isolated gram-negative bacteria from inpatients admitted at Kilimanjaro Christian Medical Centre (KCMC) between August 2013 and August 2015 were fully genome sequenced...
February 20, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Yongzhong Cao, Yongxiu Shen, Lingling Cheng, Xiaorong Zhang, Chao Wang, Yan Wang, Xiaohui Zhou, Guoxiang Chao, Yantao Wu
Salmonellae is one of the most important foodborne pathogens and becomes resistant to multiple antibiotics, which represents a significant challenge to food industry and public health. However, a molecular signature that can be used to distinguish antimicrobial resistance profile, particularly multi-drug resistance or extensive-drug resistance (XDR). In the current study, 168 isolates from the chicken and pork production chains and ill chickens were characterized by serotyping, antimicrobial susceptibility test, multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE)...
March 2018: Microbiological Research
Nafiseh Maleki, Zahra Tahanasab, Sina Mobasherizadeh, Aliakbar Rezaei, Jamshid Faghri
Background: Extended-spectrum β-lactamase (ESBL)-producing is a significant resistant mechanism to β-lactams in Enterobacteriaceae , especially in Klebsiella pneumoniae . The main objectives of this study were to genetically characterize urinary clinical isolates of K. pneumoniae through the investigating of blaTEM , blaCTX-M and using molecular typing by Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) method. We also determined the frequency of antibiotic resistance of K...
2018: Advanced Biomedical Research
Yacoub R Nairoukh, Azmi M Mahafzah, Amal Irshaid, Asem A Shehabi
Background: Emergence of multi-drug resistant uropathogenic E. coli strains is an increasing problem to empirical treatment of urinary tract infections in many countries. This study investigated the magnitude of this problem in Jordan. Methods: A total of 262 E. coli isolates were recovered from urine samples of Jordanian patients which were suspected to have urinary tract infections (UTIs). All isolates were primarily identified by routine biochemical tests and tested for antimicrobial susceptibility by disc diffusion method...
2018: Open Microbiology Journal
Ahmed Olowo-Okere, Yakubu Kokori Enevene Ibrahim, Busayo Olalekan Olayinka
OBJECTIVE: This study aimed to characterize ESBL-producing Gram-negative bacterial isolates from patients with surgical site infections (SSIs) at a tertiary healthcare facility in Abuja, Nigeria. METHOD: Consecutive, non-duplicate, wound swabs were collected, over three months period, from wounds of patients who had SSI and cultured appropriately. Bacterial isolates were identified using rapid identification kits. Modified disc diffusion method was used for antibiotics susceptibility testing and the phenotypic ESBL activity of the isolates was determined using the Double Discs Synergy Test (DDST)...
February 10, 2018: Journal of Global Antimicrobial Resistance
Azimun Nahar, Sharda Prasad Awasthi, Noritoshi Hatanaka, Kentaro Okuno, Phuong Hoai Hoang, Jayedul Hassan, Atsushi Hinenoya, Shinji Yamasaki
The purpose of this study was to investigate the prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBL-Ec) in retail chicken meats in Japan. Fifty-six domestic and 50 imported (Brazil, n=36; United States, n=8; Thailand, n=6) chicken meat samples were analyzed. The 162 ESBL-Ec included 111 from 43 (77%) domestic samples and 51 from 26 (52%) Brazilian samples. Fifty-three and 30 of 111 and 51 ESBL-Ec from domestic and Brazilian chickens, respectively, were selected for ESBL genotyping...
February 12, 2018: Journal of Veterinary Medical Science
Moisés Francisco, Sofia Santos Costa, Adriana Belas, Jorge Ramos, Isabel Couto, Constança Pomba, Miguel Viveiros
OBJECTIVES: Typhoid fever is a common infection in Africa and in spite of scarce surveillance reports, its incidence is commonly considered high by the Angolan Health system. Drug-resistant Salmonella enterica serotype Typhi has emerged, turning antimicrobial susceptibility testing essential to provide clinical guidance. This is the first report analyzing antimicrobial resistance patterns and population structure of the few S. enterica ser. Typhi isolated from patients with Typhoid fever in Luanda, Angola...
February 9, 2018: Journal of Global Antimicrobial Resistance
Babafela Awosile, J McClure, Javier Sanchez, Juan Carlos Rodriguez-Lecompte, Greg Keefe, Luke C Heider
This study was carried out to determine the frequency of fecal carriage, antimicrobial susceptibility, and resistance genes in Salmonella enterica and Escherichia coli with reduced susceptibility to extended-spectrum cephalosporins (ESC) isolated from 488 dairy calves from 8 farms in New Brunswick, Canada. Both S. enterica and E. coli with reduced susceptibility to ESC were isolated using selective culture. Minimum inhibitory concentrations to a panel of antimicrobial drugs were determined for randomly selected E...
February 7, 2018: Journal of Dairy Science
Wencheng Zou, Caiwu Li, Xin Yang, Yongxiang Wang, Guangyang Cheng, Jinxin Zeng, Xiuzhong Zhang, Yanpeng Chen, Run Cai, Qianru Huang, Lan Feng, Hongning Wang, Desheng Li, Guiquan Zhang, Yanxi Chen, Zhizhong Zhang, Heming Zhang
Escherichia coli (E. coli) is considered as a common opportunistic pathogen, which causes seriously intestinal infections to giant pandas (Ailuropoda melanoleuca) and other animals. The aim of this investigation was to characterize the antimicrobial resistance and integron gene cassettes in E. coli isolated from the faeces of giant pandas in China. A total of 89 E. coli were isolated, after diagnosis of isolates and genomes were extracted. All the isolates were screened for the presence of related drug-resistance genes and integron gene cassettes through the Polymerase Chain Reaction (PCR) and sequencing...
January 25, 2018: Microbial Pathogenesis
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