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https://www.readbyqxmd.com/read/28446899/clonal-spread-of-16s-rrna-methyltransferase-producing-klebsiella-pneumoniae-st37-with-high-prevalence-of-esbls-from-companion-animals-in-china
#1
Jing Xia, Liang-Xing Fang, Ke Cheng, Guo-Hao Xu, Xi-Ran Wang, Xiao-Ping Liao, Ya-Hong Liu, Jian Sun
We screened 30 Klebsiella pneumoniae isolates from dogs and cats at a single animal hospital in Guangdong Province, China. Among them, 12 K. pneumoniae strains possessed high-level resistance to amikacin and gentamicin and these were screened for 16S rRNA methyltransferase (16S-RMTase) genes. And then the genes positive isolates were detected for ESBLs (extended spectrum β-lactamases) and analyzed by pulsed-field gel electrophoresis, multilocus sequence typing, PCR-based replicon typing and plasmid analysis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28439298/multi-drug-resistant-pathogenic-bacteria-in-the-gut-of-young-children-in-bangladesh
#2
Shirajum Monira, Syeda Antara Shabnam, Sk Imran Ali, Abdus Sadique, Fatema-Tuz Johura, Kazi Zillur Rahman, Nur Haque Alam, Haruo Watanabe, Munirul Alam
BACKGROUND: The gut of human harbors diverse commensal microbiota performing an array of beneficial role for the hosts. In the present study, the major commensal gut bacteria isolated by culturing methods from 15 children of moderate income families, aged between 10 and 24 months, were studied for their response to different antibiotics, and the molecular basis of drug resistance. RESULTS: Of 122 bacterial colonies primarily selected from Luria-Bertani agar, bacterial genera confirmed by analytical profile index (API) 20E(®) system included Escherichia as the predominant (52%) organism, followed by Enterobacter (16%), Pseudomonas (12%), Klebsiella (6%), Pantoea (6%), Vibrio (3%), and Citrobacter (3%); while Aeromonas and Raoultella were identified as the infrequently occurring genera...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28438939/emergence-and-evolution-of-multidrug-resistant-klebsiella-pneumoniae-with-both-blakpc-and-blactx-m-integrated-in-chromosome
#3
Weihua Huang, Guiqing Wang, Robert Sebra, Jian Zhuge, Changhong Yin, Maria E Aguero-Rosenfeld, Audrey N Schuetz, Nevenka Dimitrova, John T Fallon
The extended-spectrum β-lactamase (ESBL)- and Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae represent serious and urgent threats to public health. In a retrospective study of multidrug-resistant K. pneumoniae, we identified three clinical isolates, CN1, CR14 and NY9, carrying both blaCTX-M and blaKPC genes. The complete genomes of these three K. pneumoniae isolates were de novo assembled by using both short- and long-read whole-genome sequencing. In CR14 and NY9, blaCTX-M and blaKPC were carried on two different plasmids...
April 24, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28438685/genotypic-detection-of-the-blactx-m-1-gene-among-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae
#4
Amornrat Thuengern, Surakit Nathisuwan, Mullika T Chomnawang, Chanpen Wiwat, Krit Thirapanmethee
OBJECTIVES: Extended-spectrum β-lactamases (ESBLs), a group of β-lactamase enzymes produced by bacteria in the family Enterobacteriaceae, are becoming a major problem in the healthcare community worldwide. Although many attempts have been made in the detection of ESBL-producing bacteria, the cost and speed of detection remains an important challenge. Therefore, this study aimed to develop a rapid, effective and affordable method for detection of the blaCTX-M-1 ESBL gene by a loop-mediated isothermal amplification (LAMP) technique...
April 21, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28437228/first-case-of-ndm-1-producing-klebsiella-pneumoniae-in-annaba-university-hospital-algeria
#5
Amel Abderrahim, Nassima Djahmi, Charlotte Pujol, Sabina Nedjai, Mohamed Cherif Bentakouk, Djamila Kirane-Gacemi, Mazouz Dekhil, Albert Sotto, Jean-Philippe Lavigne, Alix Pantel
AIM: The aim of this study was to characterize two carbapenem-resistant Klebsiella pneumoniae isolates recovered from urine samples in a patient hospitalized at Annaba University hospital (Algeria) in 2014. RESULTS: Two K. pneumoniae isolates were studied because they proved resistant to almost all antibiotics tested with a high level resistance to ertapenem (minimum inhibitory concentration = 32 mg/L). The results of modified Hodge test and combined disk test (ROSCO Diagnostica, Taastrup, Denmark) were positive...
February 22, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28437227/emergence-of-the-uncommon-clone-st944-st78-carrying-blaoxa-40-like-and-blactx-m-like-genes-among-carbapenem-nonsusceptible-acinetobacter-baumannii-in-moscow-russia
#6
Nikolay Mayanskiy, Igor Chebotar, Natalia Alyabieva, Olga Kryzhanovskaya, Tatiana Savinova, Andrej Turenok, Yulia Bocharova, Anna Lazareva, Svetlana Polikarpova, Olga Karaseva
Carbapenem-nonsusceptible (Carba-NS) Acinetobacter baumannii has emerged as an important cause of nosocomial infections. In the present study, we characterized 91 Carba-NS A. baumannii isolates collected from patients of surgical departments and intensive care units at three hospitals in Moscow in 2012-2015. Multilocus sequence typing (MLST) using the Oxford (Oxf) scheme identified 16 sequence types (STs) of three clonal complexes (CCs), including CC92(Oxf) (67%), CC109(Oxf) (1%), CC944(Oxf) (29%), and the singleton ST1100(Oxf) (3%)...
February 21, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28424761/sequencing-of-blaimp-carrying-incn2-plasmids-and-comparative-genomics-of-incn2-plasmids-harboring-class-1-integrons
#7
Xiaoyuan Jiang, Zhe Yin, Xiuyun Yin, Haihong Fang, Qiang Sun, Yigang Tong, Yuanhong Xu, Defu Zhang, Jiao Feng, Weijun Chen, Yajun Song, Jinglin Wang, Shuiping Chen, Dongsheng Zhou
This work presents the complete nucleotide sequences of p0801-IMP from Klebsiella pneumoniae, p7121-IMP from K. oxytoca, and p17285-IMP from Citrobacter freundii, which are recovered from three different cases of nosocomial infection. These three plasmids represent the first fully sequenced blaIMP-carrying IncN2 plasmids. Further comparative genomics analysis of all the five integron-carrying IncN2 plasmids p0801-IMP, p7121-IMP, p17285-IMP, pJIE137, and p34983-59.134kb indicates that they possess conserved IncN2 backbones with limited genetic variations with respect to gene content and organization...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28420242/high-prevalence-of-ndm-metallo-%C3%AE-lactamase-among-esbl-producing-escherichia-coli-%C3%A4-solates
#8
Yeliz Çetinkol, Cemal Sandalli, Mustafa Kerem Çalgin, Arzu Altunçekiç Yildirim, Esma Akyildiz, Esin Karaman, Ayşegül Çopur Çiçek
Resistance to β-lactams in Enterobacteriaceae has been increasing worldwide. This study aimed to determine the frequency of β-lactamase genes and antibiotic resistance rates of 140 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates obtained from urinary tract infection in Ordu Province, Turkey. Isolates were identified by classic methods and by automated system. ESBL production was confirmed by double disk synergy test and antimicrobial susceptibility was investigated by disk diffusion method...
April 19, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28419869/allodemic-distribution-of-plasmids-co-harbouring-blactx-m-15-aac-6-ib-cr-qnrb-in-klebsiella-pneumoniae-is-the-main-source-of-extended-spectrum-%C3%AE-lactamases-in-uruguay-s-paediatric-hospital
#9
V Garcia-Fulgueiras, L Araujo, I Bado, N F Cordeiro, M I Mota, G Laguna, G Algorta, R Vignoli
OBJECTIVES: This study aimed to describe the characteristics of clinical isolates of extended-spectrum β-lactamase (ESBL)-producing enterobacteria (EPE) in Uruguay's paediatric hospital. METHODS: ESBLs, qnr alleles and aac(6')-Ib-cr were sought and characterised in EPE isolated between March 2010 and March 2012. Transfer of resistance determinants was assessed by conjugation. Incompatibility (Inc) groups, plasmid toxin-antitoxin systems (TAS) and plasmid size were determined in transconjugants...
April 15, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28416556/colistin-resistant-mcr-1-positive-escherichia-coli-in-public-beaches-an-infectious-threat-emerging-in-recreational-waters
#10
Miriam R Fernandes, Fábio P Sellera, Fernanda Esposito, Caetano P Sabino, Louise Cerdeira, Nilton Lincopan
The emergence and rapid spread of colistin-resistant Escherichia coli carrying the mcr-1 gene has generated an urgent need to strengthen surveillance. We have performed a meticulous investigation of strains of this sort, resulting in the first identification of international clones of E. coli carrying IncX4 plasmid-mediated mcr-1 and blaCTX-M genes in recreational waters of public urban beaches in cities with high tourist turnover, highlighting a new environmental reservoir.
April 17, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28402509/identification-of-is1r-and-is10r-elements-inserted-into-ompk36-porin-gene-of-two-multidrug-resistant-klebsiella-pneumoniae-hospital-strains
#11
Anastasia I Lev, Evgeny I Astashkin, Rima Z Shaikhutdinova, Mikhail E Platonov, Nikolay N Kartsev, Nikolay V Volozhantsev, Olga N Ershova, Edward A Svetoch, Nadezhda K Fursova
Hospital Klebsiella pneumoniae strains (n = 196) were collected in 2012-16 from the patients of Moscow neurosurgical intensive care unit. K. pneumoniae strains were multi drug resistant (MDR) and carried beta-lactamase genes blaSHV (97.4% strains), blaCTX-M (84.7%), blaTEM (56.1%), blaOXA-48-like (49.0%), and blaNDM-1 (one strain), class 1 integrons (43.4% strains) and porin protein ompK36 gene (100% strains). The ompK36 porin protein gene disruption by IS-elements and OmpK36 production loss in two strains were detected in this study...
April 11, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28400764/antimicrobial-resistance-of-salmonella-enterica-serovar-typhimurium-in-shanghai-china
#12
Jinyan Wang, Yongrui Li, Xuebin Xu, Beibei Liang, Fuli Wu, Xiaoxia Yang, Qiuxia Ma, Chaojie Yang, Xiaofeng Hu, Hongbo Liu, Hao Li, Chunyu Sheng, Jing Xie, Xinying Du, Rongzhang Hao, Shaofu Qiu, Hongbin Song
We aimed to analyze the antimicrobial resistance phenotypes and to elucidate the molecular mechanisms underlying resistance to cephalosporins, ciprofloxacin, and azithromycin in Salmonella enterica serovar Typhimurium isolates identified from patients with diarrhea in Shanghai. The isolates showed high rates of resistance to traditional antimicrobials, and 20.6, 12.7, and 5.5% of them exhibited decreased susceptibility to cephalosporins, ciprofloxacin, and azithromycin, respectively. Notably, 473 (84.6%) isolates exhibited multidrug resistance (MDR), including 161 (28...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28396551/specific-blactx-m-8-inci1-plasmid-transfer-among-genetically-diverse-escherichia-coli-across-humans-and-chickens
#13
Chihiro Norizuki, Jun-Ichi Wachino, Masahiro Suzuki, Kumiko Kawamura, Noriyuki Nagano, Kouji Kimura, Yoshichika Arakawa
We investigated the genetic backbones of 14 blaCTX-M-8-positive Escherichia coli isolates recovered from human stools and chicken meat. All isolates carried IncI1 plasmids with blaCTX-M-8 (blaCTX-M-8/IncI1), and most (9/14) belonged to a specific genetic lineage, namely, plasmid sequence type (pST) 113. The genetic contexts of the 9 blaCTX-M-8/IncI1-pST113 were similar, regardless of the source. These results suggest probable local transfer of the blaCTX-M-8/IncI1 between humans and chicken with genetically diverse E...
April 10, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28384061/extended-spectrum-cephalosporin-resistant-enterobacteriaceae-in-enteric-microflora-of-wild-ducks
#14
Dimitria A Mathys, Dixie F Mollenkopf, Jacqueline Nolting, Andrew S Bowman, Joshua B Daniels, Thomas E Wittum
We tested 772 cloacal swabs from wild ducks to estimate the prevalence of enteric bacteria resistant to extended-spectrum cephalosporins (ESC). We found a low prevalence of the important ESC resistance genotypes, blaCMY (5.7%) and blaCTX-M (0.3%). This suggests a minor role for wild ducks in the movement of resistant bacteria in the environment.
April 6, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28383517/antibiotic-resistance-in-an-indian-rural-community-a-one-health-observational-study-on-commensal-coliform-from-humans-animals-and-water
#15
Manju Raj Purohit, Salesh Chandran, Harshada Shah, Vishal Diwan, Ashok J Tamhankar, Cecilia Stålsby Lundborg
Antibiotic-resistant bacteria are an escalating grim menace to global public health. Our aim is to phenotype and genotype antibiotic-resistant commensal Escherichia coli (E. coli) from humans, animals, and water from the same community with a 'one-health' approach. The samples were collected from a village belonging to demographic surveillance site of Ruxmaniben Deepchand (R.D.) Gardi Medical College Ujjain, Central India. Commensal coliforms from stool samples from children aged 1-3 years and their environment (animals, drinking water from children's households, common source- and waste-water) were studied for antibiotic susceptibility and plasmid-encoded resistance genes...
April 6, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28380311/spread-of-ctx-type-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-isolates-of-epidemic-clone-b2-o25-st131-among-dogs-and-cats-in-japan
#16
Kumiko Kawamura, Tae Sugawara, Nao Matsuo, Kengo Hayashi, Chihiro Norizuki, Kiyoko Tamai, Takaaki Kondo, Yoshichika Arakawa
This study was performed to investigate the carriage rates of CTX-M-type extended-spectrum β-lactamase (ESBL)-producing Escherichia coli among ill companion animals in Japan. Among the 178 nonrepetitive E. coli isolates, including 131 from dogs and 47 from cats, collected between September and November 2015, 42 (23.6%) isolates from 29 dogs and 13 cats were identified as ESBL producers. The antimicrobial susceptibility, O serotype, phylogenetic group, β-lactamase genotype, plasmid replicon type, and sequence type (ST) of each isolate were analyzed...
April 5, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28379732/prevalence-of-plasmid-mediated-fosfomycin-resistance-gene-fosa3-among-ctx-m-producing-escherichia-coli-isolates-from-chickens-in-china
#17
Wei Jiang, Shuai Men, Linghan Kong, Suzhen Ma, Yongqiang Yang, Yongxiang Wang, Qiwu Yuan, Guangyang Cheng, Wencheng Zou, Hongning Wang
The aim of this study was to investigate the prevalence of fosfomycin resistance gene fosA3 and characterize plasmids harboring fosA3 among CTX-M-producing Escherichia coli from chickens in China. A total of 234 CTX-M-producing E. coli isolates collected from chickens from 2014 to 2016 were screened for the presence of plasmid-mediated fosfomycin resistance genes (fosA, fosA3, and fosC2). Clonal relatedness of fosA3-positive isolates was determined by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST)...
April 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28375698/extended-spectrum-%C3%AE-lactamase-producing-klebsiella-pneumoniae-from-pharmaceutical-wastewaters-in-south-western-nigeria
#18
Avemaria Obasi, SimonCyril Nwachukwu, Esther Ugoji, Christian Kohler, André Göhler, Veronica Balau, Yvonne Pfeifer, Ivo Steinmetz
Emergence and spread of Klebsiella pneumoniae isolates producing extended-spectrum β-lactamases (ESBLs) present a major threat to public health. In this study, we characterized β-lactam-resistant K. pneumoniae isolates from six wastewater samples obtained from two pharmaceutical industries located in Lagos and Ogun States, Nigeria. Bacteria were isolated by using MacConkey agar; species identification and antibacterial susceptibility testing were performed by Vitek 2. Etest was used for ESBL phenotype confirmation...
April 4, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28373192/a-waterborne-outbreak-of-shigella-sonnei-with-resistance-to-azithromycin-and-third-generation-cephalosporins-in-china-2015
#19
Qiuxia Ma, Ming Luo, Xuebin Xu, Jian Wang, Chaojie Yang, Xiaofeng Hu, Beibei Liang, Fuli Wu, Xiaoxia Yang, Jinyan Wang, Hongbo Liu, Wen Li, Yu Zhong, Peng Li, Jing Xie, Leili Jia, Ligui Wang, Rongzhang Hao, Xinying Du, Shaofu Qiu, Hongbin Song, Yansong Sun
Here we for the first time reported a waterborne outbreak of Shigella sonnei in China, 2015. Eleven multidrug resistance (MDR) S. sonnei isolates were recovered, showing highly resistant to azithromycin and third-generation cephalosporins particularly, due to the mph (A) and blaCTX-M-14-encoding IncB/O/K/Z group transmissible plasmid of 104.285 kb in size. Our study highlights the potential prevalence of the MDR outbreak S. sonnei in China and its further dissemination worldwide with the development of globalization...
April 3, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28368864/characterization-of-esbl-producing-escherichia-coli-recovered-from-companion-dogs-in-tai-an-china
#20
Song Li, Junhe Liu, Yufa Zhou, Zengmin Miao
INTRODUCTION: Animals are considered to be reservoirs of extended-spectrum beta-lactamase (ESBL)-producing bacteria, but few epidemiological data on ESBL-producing Escherichia coli urinary tract isolates in pet dogs are available in China. METHODOLOGY: This study was conducted to describe the prevalence and characterization of ESBL producers among E. coli urinary tract isolates from pet dogs in Tai'an, China. RESULTS: A total of 118 E. coli were obtained from urinary samples of 80 companion dogs suffering from acute or chronic cystitis, of which three isolates from different dogs were ESBL producers...
March 31, 2017: Journal of Infection in Developing Countries
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