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Acinetobacter calcoaceticus

Mónica Cerezales, Kyriaki Xanthopoulou, Julia Ertel, Alexandr Nemec, Zulema Bustamante, Harald Seifert, Lucia Gallego, Paul G Higgins
Acinetobacter seifertii is a recently described species that belongs to the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. It has been recovered from clinical samples and is sometimes associated with antimicrobial resistance determinants. We present here the case of three A. seifertii clinical isolates which were initially identified as Acinetobacter sp. by phenotypic methods but no identification at the species level was achieved using semi-automated identification methods. The isolates were further analysed by whole genome sequencing and identified as A...
May 8, 2018: Journal of Medical Microbiology
Rakshya Nepal, Basudha Shrestha, Deepak Man Joshi, Rajesh Dhoj Joshi, Sanjit Shrestha, Anjana Singh
BACKGROUND: Lower respiratory tract infection is a common cause of morbidity and mortality worldwide. A cross-sectional study was carried out with an objective to study the antibiogram of Gram-negative isolates of patients with lower respiratory tract infection visiting Kathmandu Model Hospital. METHODS: A total of 274 specimens including sputum, endotracheal aspirates, suction tips were cultured as per standard microbiological technique. Antibiotic susceptibility and detection of Extended-spectrum beta- lactamases (ESBLs) were performed following Clinical Laboratory Standard Institute (CLSI 2014) guidelines...
March 13, 2018: Journal of Nepal Health Research Council
Rómel Fabian Gómez, Andres Castillo, Mónica Chávez-Vivas
Introduction: The extensive use of antibiotics has led to the emergence of multi-resistant strains in some species of the genus Acinetobacter . Objective: To investigate the molecular characteristics of multidrug-resistant of Acinetobacter ssp. strains isolated from 52 patients collected between March 2009 and July 2010 in medical intensive care units in Cali - Colombia. Methods: The susceptibility to various classes of antibiotics was determined by disc diffusion method, and the determination of the genomic species was carried out using amplified ribosomal DNA restriction analysis (ARDRA) and by sequencing of the 16s rDNA gene...
December 30, 2017: Colombia Médica: CM
Kerdalidec Candy, Nadia Amanzougaghene, Arezki Izri, Sophie Brun, Rémy Durand, Meriem Louni, Didier Raoult, Florence Fenollar, Oleg Mediannikov
Human lice, Pediculus humanus, are obligate blood-sucking parasites. Phylogenetically, they belong to several mitochondrial clades exhibiting some geographic differences. Currently, the body louse is the only recognized disease vector, with the head louse being proposed as an additional vector. In this article, we study the genetic diversity of head and body lice collected from Bobigny, a town located close to Paris (France), and look for louse-borne pathogens. By amplifying and sequencing the cytb gene, we confirmed the presence of clades A and B in France...
March 20, 2018: Vector Borne and Zoonotic Diseases
A Ramette, A Kronenberg
BACKGROUND: We describe the prevalence of invasive carbapenem-resistant Acinetobacter spp. isolated from 2005 to 2016 in different regions of Switzerland. METHODS: Using the Swiss Antibiotic Resistance Centre (anresis) database that includes data from 70% of all hospitalized patients and one third of all ambulatory practitioners in Switzerland, we analysed the number of carbapenem-susceptible and resistant Acinetobacter spp. isolated from blood or cerebrospinal fluid, and further described their temporal and regional fluctuations...
April 3, 2018: BMC Infectious Diseases
Yonghua Liu, Xinxin Hu, Yanbin Wu, Weixing Zhang, Xiaofang Chen, Xuefu You, Laixing Hu
A series of novel diamidines with N-substituents on an amidine N-atom were synthesized and evaluated for their cytotoxicity and in vitro antibacterial activity against a range of Gram-positive and Gram-negative bacterial strains. Based on structure-activity relationship, N-substituents with a branched chain and a shorter carbon chain on the amidine N-atom exhibited more promising activity against Gram-negative and MDR-Gram-positive bacteria; compounds 5c and 5i were the most powerful candidate compounds. Compound 5c showed greater efficacy than levofloxacin against most drug-resistant Gram-positive bacteria and exhibited broad-spectrum antibacterial activity against Gram-negative bacteria, with MIC values in the range of 2-16 μg/mL...
March 12, 2018: European Journal of Medicinal Chemistry
Gonzalo de Gonzalo, Antonio Franconetti
The recently discovered cyclohexanone monooxygenase from Thermocrispum municipale (TmCHMO) was employed in the preparation of different optically active sulfoxides. This novel Baeyer-Villiger monooxygenase (BVMO) shows a similar substrate specificity and selectivity when compared with the prototype CHMO from Acinetobacter calcoaceticus. Interestingly, TmCHMO is able to catalyse the asymmetric sulfoxidations at relatively high temperatures, substrate concentrations and in the presence of organic solvents while maintaining or even increasing its selectivity...
June 2018: Enzyme and Microbial Technology
Hélène Pailhoriès, Clémence Tiry, Matthieu Eveillard, Marie Kempf
This study assessed the characteristics of hospital-acquired Acinetobacter calcoaceticus - Acinetobacter baumannii complex (ACB) bloodstream infections (BSI). From 2010 to 2017, ACB BSI were investigated. Of 73 cases, 54.8% were associated with A. pittii, 39.7% with A. baumannii and 5.5% with A. nosocomialis. MDR isolates were significantly higher in A. baumannii BSI. The 30-day mortality was no different between A. baumannii or non-baumannii ACB BSI. In contrast with other studies, here, most of hospital-acquired ACB BSI were associated with non-baumannii ACB species...
March 22, 2018: Journal of Hospital Infection
Maria Botou, Panayiota Lazou, Konstantinos Papakostas, George Lambrinidis, Thomas Evangelidis, Emmanuel Mikros, Stathis Frillingos
The uracil permease UraA of Escherichia coli is a structurally known prototype for the ubiquitous Nucleobase-Ascorbate Transporter (NAT) or Nucleobase-Cation Symporter-2 (NCS2) family and represents a well-defined subgroup of bacterial homologs that remain functionally unstudied. Here, we analyze four of these homologs, including RutG of E. coli which shares 35% identity with UraA and is encoded in the catabolic rut (pyrimidine utilization) operon. Using amplified expression in E. coli K-12, we show that RutG is a high-affinity permease for uracil, thymine and, at low efficiency, xanthine and recognizes also 5-fluorouracil and oxypurinol...
April 2018: Molecular Microbiology
Kayleigh E Mitchell, Jane F Turton, David H Lloyd
BACKGROUND: Acinetobacter species can exhibit widespread resistance to antimicrobial agents. They are already recognized as important nosocomial pathogens of humans, but are becoming increasingly recognized in opportunistic infections of animals. HYPOTHESIS/OBJECTIVES: This study aimed to determine whether Acinetobacter spp. are carried on skin of healthy dogs and, if present, to identify the species. ANIMALS: Forty dogs were sampled at veterinary practices and rescue centres...
February 11, 2018: Veterinary Dermatology
Yan Chen, Zhaoming Liu, Hongju Liu, Yahong Pan, Jing Li, Lan Liu, Zhigang She
Three new isocoumarins-dichlorodiaportintone ( 1 ), desmethyldichlorodiaportintone ( 2 ) and desmethyldichlorodiaportinol ( 3 )-as well as six known analogues ( 4-9 ) were isolated from the culture of the mangrove endophytic fungus Ascomycota sp. CYSK-4 from Pluchea indica . Their structures were elucidated by analysis of spectroscopic data. The absolute configuration of compounds 1 and 2 were determined by the modified Mosher's method. Compound 2 showed significant anti-inflammatory activity by inhibiting the production of NO in LPS-induced RAW 264...
February 9, 2018: Marine Drugs
Young Ah Kim, Jin Ju Kim, Dong Ju Won, Kyungwon Lee
Identifying the trends in community-onset Acinetobacter baumannii complex isolation and diversity according to temperature could help provide insight into the behavior of the A. baumannii complex. We performed a retrospective analysis of A. baumannii complex (Acinetobacter baumannii, Acinetobacter nosocomialis, Acinetobacter pittii, and Acinetobacter calcoaceticus) isolates obtained from patients at a Korean community hospital from 2006 to 2015 with reference to seasonal temperatures. The incidence rates were compared between warm (June-September) and cold (November-March) months, defined as an average mean temperature ≥20°C and ≤5°C, respectively...
May 2018: Annals of Laboratory Medicine
Charlotte Morrill, Chantel Jensen, Xavier Just-Baringo, Gideon Grogan, Nicholas J Turner, David J Procter
Cyclic ketones bearing α-quaternary stereocenters underwent efficient kinetic resolution using cyclohexanone monooxygenase (CHMO) from Acinetobacter calcoaceticus. Lactones possessing tetrasubstituted stereocenters were obtained with high enantioselectivity (up to >99 % ee) and complete chemoselectivity. Preparative-scale biotransformations were exploited in conjunction with a SmI2 -mediated cyclization process to access complex, enantiomerically enriched cycloheptan- and cycloctan-1,4-diols. In a parallel approach to structurally distinct products, enantiomerically enriched ketones from the resolution with an α-quaternary stereocenter were used in a SmI2 -mediated cyclization process to give cyclobutanol products (up to >99 % ee)...
March 26, 2018: Angewandte Chemie
Lu Chen, Juxiang Yuan, Yingjun Xu, Fengxia Zhang, Zhenlei Chen
The Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex has emerged as a high priority among hospital-acquired pathogens in intensive care units (ICUs), posing a challenge to infection management practices. In this study, the clinical characteristics, antimicrobial susceptibility patterns, and patients outcome among genospecies were retrospectively compared. Samples were taken from the tracheal secretions of 143 patients in the ICU. Genospecies of the ACB complex were discriminated by analysis of the 16S-23S rRNA gene intergenic spacer (ITS) sequence...
2018: PloS One
Yulia Rosa Saharman, Anis Karuniawati, Rudyanto Sedono, Dita Aditianingsih, Pratiwi Sudarmono, Wil H F Goessens, Corné H W Klaassen, Henri A Verbrugh, Juliëtte A Severin
Background: Carbapenem-nonsusceptible A. baumannii-calcoaceticus complex have emerged worldwide, but the epidemiology in Indonesian hospitals has not been studied. Methods: A prospective observational study was performed on the intensive care units (ICUs) of the national referral hospital in Jakarta-Indonesia, in 2013 and 2014. All consecutive adult patients admitted and hospitalized for >48 h in ICUs were included. Basic and clinical data at admission were recorded...
2018: Antimicrobial Resistance and Infection Control
Massimiliano Lucidi, Federica Runci, Giordano Rampioni, Emanuela Frangipani, Livia Leoni, Paolo Visca
Understanding bacterial pathogenesis requires adequate genetic tools to assess the role of individual virulence determinants by mutagenesis and complementation assays, as well as for homologous and heterologous expression of cloned genes. Our knowledge of Acinetobacter baumannii pathogenesis has so far been limited by the scarcity of genetic tools to manipulate multidrug-resistant (MDR) epidemic strains, which are responsible for most infections. Here, we report on the construction of new multipurpose shuttle plasmids, namely, pVRL1 and pVRL2, which can efficiently replicate in Acinetobacter spp...
April 2018: Antimicrobial Agents and Chemotherapy
Bruna Kern Donamore, Stephanie Wagner Gallo, Pedro Maria Abreu Ferreira, Carlos Alexandre Sanchez Ferreira, Sílvia Dias de Oliveira
AIM: To evaluate the influence of aeration on persister levels from Acinetobacter calcoaceticus-baumannii isolates exposed to meropenem or tobramycin, as well as analyze morphological and structural changes in persisters. MATERIALS & METHODS: Levels of persisters were determined after a 48-h exposure to tobramycin or meropenem under aerated or static conditions, and persisters were analyzed by scanning and transmission electron microscopy. RESULTS: The fractions of persisters varied between isolates...
February 2018: Future Microbiology
A C Hernandez-Morales, L L Lessor, T L Wood, D Migl, E M Mijalis, W K Russell, R F Young, J J Gill
The increased prevalence of drug-resistant, nosocomial Acinetobacter infections, particularly from pathogenic members of the Acinetobacter calcoaceticus-baumannii complex, necessitates the exploration of novel treatments such as phage therapy. In the present study, we characterize phage Petty, a novel podophage that infects multidrug-resistant Acinetobacter nosocomialis and Acinetobacter baumannii Genome analysis reveals that phage Petty is a 40,431bp ϕKMV-like phage, with a coding density of 92.2% and a G+C content of 42...
January 3, 2018: Journal of Virology
LongFei Zhao, YaJun Xu, XinHe Lai
A total of 276 endophytic bacteria were isolated from the root nodules of soybean (Glycine max L.) grown in 14 sites in Henan Province, China. The inhibitory activity of these bacteria against pathogenic fungus Phytophthora sojae 01 was screened in vitro. Six strains with more than 63% inhibitory activities were further characterized through optical epifluorescence microscopic observation, sequencing, and phylogenetic analysis of 16S rRNA gene, potential plant growth-promoting properties analysis, and plant inoculation assay...
October 13, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
Hugo Oliveira, Ana R Costa, Nico Konstantinides, Alice Ferreira, Ergun Akturk, Sanna Sillankorva, Alexandr Nemec, Mikhail Shneider, Andreas Dötsch, Joana Azeredo
Bacteriophages are ubiquitous in nature and represent a vast repository of genetic diversity, which is driven by the endless coevolution cycle with a diversified group of bacterial hosts. Studying phage-host interactions is important to gain novel insights into their dynamic adaptation. In this study, we isolated 12 phages infecting species of the Acinetobacter baumannii-Acinetobacter calcoaceticus complex which exhibited a narrow host range and similar morphological features (podoviruses with short tails of 9-12 nm and isometric heads of 50-60 nm)...
December 2017: Environmental Microbiology
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