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https://www.readbyqxmd.com/read/28545055/comparative-lytic-efficacy-of-rt-pa-and-ultrasound-in-porcine-versus-human-clots
#1
Shenwen Huang, Himanshu Shekhar, Christy K Holland
INTRODUCTION: Porcine thrombi are employed routinely in preclinical models of ischemic stroke. In this study, we examined the differential lytic susceptibility of porcine and human whole blood clots with and without the use of microbubbles and ultrasound (US) as an adjuvant. MATERIALS AND METHODS: An in vitro system equipped with time-lapse microscopy was used to evaluate recombinant tissue-plasminogen activator (rt-PA) lysis of porcine and human clots in the same species or cross species plasma...
2017: PloS One
https://www.readbyqxmd.com/read/28543387/the-hematocrit-affects-the-volume-of-plasma-treated-with-coupled-plasma-filtration-and-adsorption-with-predilution
#2
Stefano Finazzi, Elena Garbero, Giampietro Trussardi, Guido Bertolini
Coupled plasma filtration and adsorption (CPFA) is an extracorporeal blood purification technique proposed for the treatment of septic-shock. By removing pro- and anti-inflammatory mediators from plasma, CPFA is supposed to have a therapeutic effect on the abnormal inflammatory response seen in this condition. Recently, blood predilution with citrate solution has been adopted to prevent clotting in the CPFA circuit-one of the main problems of the technique. Taking into account the patient's hematocrit, we worked out a formula for the volume of plasma effectively treated by CPFA after predilution...
May 2017: Artificial Organs
https://www.readbyqxmd.com/read/28541681/platelet-microcapsule-hybrids-leverage-contractile-force-for-targeted-delivery-of-hemostatic-agents
#3
Caroline E Hansen, David R Myers, Wallace Hunter Baldwin, Yumiko Sakurai, Shannon L Meeks, L Andrew Lyon, Wilbur A Lam
We report a cell-mediated, targeted drug delivery system utilizing polyelectrolyte multilayer capsules that hybridize with the patient's own platelets upon intravenous administration. The hybridized platelets function as the sensor and actuator for targeted drug delivery and controlled release in our system. These capsules are biochemically and mechanically tuned to enable platelet adhesion and capsule rupture upon platelet activation and contraction, enabling the targeted and controlled "burst" release of an encapsulated biotherapeutic...
May 25, 2017: ACS Nano
https://www.readbyqxmd.com/read/28540073/viscoelastic-testing-inside-and-beyond-the-operating-room
#4
REVIEW
Liang Shen, Sheida Tabaie, Natalia Ivascu
Hemorrhage is a major contributor to morbidity and mortality during the perioperative period. Current methods of diagnosing coagulopathy have various limitations including long laboratory runtimes, lack of information on specific abnormalities of the coagulation cascade, lack of in vivo applicability, and lack of ability to guide the transfusion of blood products. Viscoelastic testing offers a promising solution to many of these problems. The two most-studied systems, thromboelastography (TEG) and rotational thromboelastometry (ROTEM), offer similar graphical and numerical representations of the initiation, formation, and lysis of clot...
April 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28539608/development-and-validation-of-a-high-throughput-whole-blood-thrombolysis-plate-assay
#5
T Bonnard, L S Law, Z Tennant, C E Hagemeyer
The objective of this work was to develop a high throughput assay for testing in vitro the thrombolytic activity using citrated whole blood samples, and to overcome the limitations of currently available techniques. We successfully developed a method that involves forming halo shaped, tissue factor induced, whole blood clots in 96 well plates, and then precisely measuring the thrombolysis process with a spectrophotometer plate reader. We here describe the implementation of this novel method, which we refer to as halo assay, and its validation with plasmin, urokinase and tissue plasminogen activator at different doses...
May 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28536753/differentiation-of-clot-composition-using-conventional-and-dual-energy-computed-tomography
#6
Jan Borggrefe, Jonathan Kottlors, Mahmood Mirza, Victor-Frederic Neuhaus, Nuran Abdullayev, Volker Maus, Christoph Kabbasch, David Maintz, Anastasios Mpotsaris
BACKGROUND AND PURPOSE: In unenhanced computed tomography (CT) of acute ischemic stroke, the density of occluding clots is associated with the content of red blood cells and successful recanalization with stent thrombectomy. However, no CT marker for fibrin content is established. In order to improve clot diagnostics, we conducted an in vitro study to investigate thrombus composition of histologically defined ovine blood clots with unenhanced and contrast-enhanced CT using spectral detector CT (SDCT)...
May 23, 2017: Clinical Neuroradiology
https://www.readbyqxmd.com/read/28536427/role-of-foxo1-and-apoptosis-in-pulmonary-vascular-remolding-in-a-rat-model-of-chronic-thromboembolic-pulmonary-hypertension
#7
Chaosheng Deng, Zhanghua Zhong, Dawen Wu, Yunfei Chen, Ningfang Lian, Haibo Ding, Qiaoxian Zhang, Qichang Lin, Shuang Wu
To explore the role of FoxO1 and apoptosis in a rat model of chronic thromboembolic pulmonary hypertension (CTEPH). Rats were randomly divided into a sham group (n = 45) and an experimental group (n = 45). Autologous blood clots were injected into rats three times to induce CTEPH. Rats were further divided into three subgroups: a 1-week subgroup (n = 15), a 2-week subgroup (n = 15), and a 4-week subgroup (n = 15). Mean pulmonary arterial pressure (mPAP) and histopathology were evaluated at each time point...
May 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28533072/relationship-between-transfusion-of-blood-products-and-the-incidence-of-thrombotic-complications-in-neonates-and-infants-undergoing-cardiac-surgery
#8
David Faraoni, Sirisha Emani, Erin Halpin, Rachel Bernier, Sitaram Emani, James A DiNardo, Juan C Ibla
OBJECTIVES: The authors hypothesized that transfusion of blood products in neonates and infants undergoing high-risk cardiac surgery in the absence of intraoperative coagulation monitoring increases the risk of thrombotic complications. DESIGN: Prospective observational study. SETTING: Neonates and infants undergoing cardiac surgery at a tertiary pediatric center. PARTICIPANTS: Neonates weighing >2.5 kg and infants ≤12 months of age undergoing elective cardiac surgery with cardiopulmonary bypass were included in this prospective observational study...
April 27, 2017: Journal of Cardiothoracic and Vascular Anesthesia
https://www.readbyqxmd.com/read/28532176/synthesis-of-n-alkylated-chitosan-and-its-interactions-with-blood
#9
Yuchen Huang, Yi Zhang, Longbao Feng, Liumin He, Rui Guo, Wei Xue
When hydrophobically modified chitosan (HM-CHI) comes into contact with red blood cells (RBCs), it can cause agglomeration. This property leads HM-CHI to be a potential agent to form a rapid haemostatic plug to stop bleeding at wound sites. In this study, we investigated the properties of the HM-CHI biopolymer to act as an agent that rapidly clots blood. We have examined the synthesis and structural characteristics of HM-CHI - blood substances using X-ray diffraction, infrared spectroscopy and elemental analysis...
May 22, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28532074/highly-effective-cu-zn-carbon-micro-nanofiber-polymer-nanocomposite-based-wound-dressing-biomaterial-against-the-p-aeruginosa-multi-and-extensively-drug-resistant-strains
#10
Mohammad Ashfaq, Nishith Verma, Suphiya Khan
Pseudomonas aeruginosa (P. aeruginosa) is the most prevalent bacteria in the infections caused by burn, surgery, and traumatic injuries. Emergence of the P. aeruginosa bacterial resistance against various clinical drugs for wound treatment is the major concern nowadays. The present study describes the synthesis of the polyvinyl alcohol (PVA) and cellulose acetate phthalate (CAP) polymeric composite film (~0.2mm thickness) reinforced with the Cu/Zn bimetal-dispersed activated carbon micro/nanofiber (ACF/CNF), as a wound dressing material...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28532047/porous-chitosan-microspheres-for-application-as-quick-in-vitro-and-in-vivo-hemostat
#11
Jixiang Li, Xiaowei Wu, Yanqing Wu, Zonghao Tang, Xun Sun, Meng Pan, Yufeng Chen, Juanjuan Li, Rongdong Xiao, Zhengchao Wang, Haiqing Liu
Controlling massive hemorrhage is of great importance to lower transfusional medical cost, and to reduce death and mobility rate in battlefield and civilian accidents. We reported the fabrication of porous chitosan microspheres (CSMS) with tunable surface pore size by microemulsion combined with thermally induced phase separation technique, and its application as a quick hemostat. Their hemostatic property was characterized by blood clotting kinetics, adherence interaction between red blood cells/platelets and CSMS, in vitro and in vivo hemostasis by rat tail amputation and liver laceration models, and histological analysis...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28530135/prolonged-expression-of-secreted-enzymes-in-dogs-after-liver-directed-delivery-of-i-sleeping-beauty-i-transposons-implications-for-non-viral-gene-therapy-of-systemic-disease
#12
Elena L Aronovich, Kendra Anne Hyland, Bryan C Hall, Jason B Bell, Erik R Olson, Myra Urness Rusten, David W Hunter, N Matthew Ellinwood, R Scott McIvor, Perry B Hackett
The non-viral, integrating Sleeping Beauty (SB) transposon system is efficient in treating systemic monogenic disease in mice including hemophilias A and B caused by deficiency of blood clotting factors and mucopolysaccharidosis (MPS) types I and VII caused by α-L-iduronidase (IDUA) and β-glucuronidase (GUSB) deficiency, respectively. We recently reported modified approaches of the hydrodynamics-based procedure to deliver transposons to the liver in dogs. Using the transgenic canine reporter secreted alkaline phosphatase (cSEAP), we demonstrated transgenic protein in the plasma for up to six weeks post-infusion...
May 20, 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/28529414/comparison-of-efficacy-of-autologous-platelet-rich-fibrin-versus-saline-dressing-in-chronic-venous-leg-ulcers-a-randomised-controlled-trial
#13
Anirudh Somani, Reena Rai
BACKGROUND: Venous leg ulcer is a chronic condition, and various treatment modalities are available. Platelet-rich fibrin (PRF) is one of the newer modalities and it contains fibroblast growth factor (GF), vascular endothelial GF, angiopoitin and platelet-derived GF which enhances the wound healing. Hence, we conducted a randomised controlled trial to compare the efficacy of PRF versus saline dressing in chronic venous leg ulcers. AIM: This study aims to compare the efficacy of autologous PRF with saline dressing in patients with chronic venous leg ulcer and to compare the mean reduction in ulcer area at the end of 4 weeks...
January 2017: Journal of Cutaneous and Aesthetic Surgery
https://www.readbyqxmd.com/read/28527902/the-provisional-matrix-setting-the-stage-for-tissue-repair-outcomes
#14
EDITORIAL
Thomas H Barker, Adam J Engler
Since its conceptualization in the 1980s, the provisional matrix has often been characterized as a simple fibrin-containing scaffold for wound healing that supports the nascent blood clot and is functionally distinct from the basement membrane. However subsequent advances have shown that this matrix is far from passive, with distinct compositional differences as the wound matures, and providing an active role for wound remodeling. Here we review the stages of this matrix, provide an update on the state of our understanding of provisional matrix, and present some of the outstanding issues related to the provisional matrix, its components, and their assembly and use in vivo...
July 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28525329/thromboelastographic-profile-of-goat-blood-after-the-experimental-injury-of-the-femoral-artery-and-use-of-quikclot-gauze-and-celox-gauze-dressings
#15
P Sobiech, Z Adamiak, P Holak, P Jastrzębski, J Rogowski, M Brzeziński, K Bury, M Jałyński, W Baumgartner
The aim of this study was to evaluate the suitability of thromboelastometry for the analysis of blood test results in goats after the use of hemostatic dressings to control massive bleeding. The study was carried out on 12 goats, 6 animals in each of two subgroups. In all experimental animals incision of the femoral artery was performed, and bleeding was controlled with QuikClot gauze in the first group and Celox gauze in the second group. Dressings were applied for 60 minutes. Blood samples for thromboelastometry were collected from the jugular vein before the incision and 60 min after the application of a dressing...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28523092/management-of-a-life-threatening-bleeding-following-extraction-of-deciduous-second-molar-related-to-a-capillary-haemangioma
#16
REVIEW
Amr Amin Ghanem, Yasser Nabil El Hadidi
Various forms of vascular lesion affect the head and neck region. The head and neck vascular lesions are classified into neoplasms and malformations. Neoplasm presents either as hemangioma or lymphangioma; neoplasm usually presents in young age compared with vascular malformation. A 9-year-old female patient presented to the outpatient clinic referred from the department of pedodontics after extraction of a right mandibular second deciduous molar. Extraction was done by dental GP in outpatient clinic. Massive bleeding followed the extraction...
June 2017: Craniomaxillofacial Trauma & Reconstruction
https://www.readbyqxmd.com/read/28522812/selection-and-characterization-of-a-dna-aptamer-inhibiting-coagulation-factor-xia
#17
David A Donkor, Varsha Bhakta, Louise J Eltringham-Smith, Alan R Stafford, Jeffrey I Weitz, William P Sheffield
Factor XIa (FXIa) is a serine protease that catalyzes the activation of Factor IX (FIX) in the blood coagulation cascade. FXIa and its precursor FXI are emergent therapeutic targets for the development of safer anticoagulant agents. Here, we sought a novel DNA-based agent to inhibit FXIa. Towards this goal, an 80 base, single-stranded DNA aptamer library (containing a 40 base randomized core) was screened for FXIa-binding candidates, using ten rounds of positive and negative selection. After selection, 6 of 89 different sequences inhibited FXIa-mediated chromogenic substrate S2366 cleavage...
May 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28521997/biodegradable-collagen-sponge-reinforced-with-chitosan-calcium-pyrophosphate-nanoflowers-for-rapid-hemostasis
#18
Tingsheng Yan, Feng Cheng, Xinjing Wei, Yudong Huang, Jinmei He
Efficient and biodegradable hemostatic materials become increasingly important in civilian and military clinical. However, traditional hemostatic materials are difficult to achieve expected effects especially in parenchymal organs with rich vascularity. In facing these challenges, we designed a biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers (CPNFs-Col sponge) for rapid hemostasis. With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952...
August 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28520166/operator-based-variability-of-equine-thromboelastography
#19
Kristen Thane, Daniela Bedenice, Ana Pacheco
OBJECTIVE: To determine the extent of intraoperator (between duplicate samples) and interoperator (between different operators) variability in equine thromboelastography (TEG). DESIGN: Kaolin-activated TEG was performed in duplicate by operator-pair A/B or A/C (2 groups of 10 horses) using discrete setups, within 30-45 minutes of vacuum-assisted blood collection. Recorded TEG variables included clot initiation time (R), clot formation time (K), rate of clot formation (α), clot strength (MA), and viscoelastic/shear strength (G)...
May 18, 2017: Journal of Veterinary Emergency and Critical Care
https://www.readbyqxmd.com/read/28513800/sociodemographic-factors-and-functional-capacity-of-elderly-affected-by-stroke
#20
Michelinne Oliveira Machado Dutra, Alexsandro Silva Coura, Inacia Sátiro Xavier de França, Bertha Cruz Enders, Mayara Araújo Rocha
Introduction: Diseases of old age have shown increasing prevalence in society. One of them is stroke, which can be conceptualized as the interruption of cerebral blood supply due to a leakage or blood vessel obstruction caused by clots. Objective: To verify the associations between sociodemographic factors and the functional capacity of elderly affected by stroke. Methods: This is an epidemiological, cross-sectional, and quantitative study, including 118 elderly people with paralyses due to stroke, who were registered in one of the Family Health Strategies units of Campina Grande, Paraíba, Brazil...
January 2017: Revista Brasileira de Epidemiologia, Brazilian Journal of Epidemiology
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