keyword
https://read.qxmd.com/read/37670968/bioinformatics-and-expression-analyses-of-mir-639-mir-641-mir-1915-3p-and-mir-3613-3p-in-colorectal-cancer-pathogenesis
#1
JOURNAL ARTICLE
Rusen Avsar, Turkan Gurer, Alper Aytekin
Objectives: MicroRNAs (miRNAs) have important function in cancer development and progression. This study aims to determine the expression levels of miR-639, miR-641, miR-1915-3p, and miR-3613-3p in tissues of colorectal cancer (CRC) patients and the role of these miRNAs in the CRC pathogenesis. Methods: Tumor and non-tumor tissues were collected from a total of 59 CRC patients. qRT-PCR was used to identify the expressions of miR-639, miR-641, miR-1915-3p and miR-3613-3p. Through bioinformatics analysis, the target genes of miRNAs were identified by using DIANA mirPath v...
2023: Journal of Cancer
https://read.qxmd.com/read/36624663/in-silico-identification-of-hypoxic-signature-followed-by-reverse-transcription-quantitative-pcr-validation-in-cancer-cell-lines
#2
JOURNAL ARTICLE
Sara Shayan, Golnaz Bahramali, Arash Arashkia, Kayhan Azadmanesh
BACKGROUND: Hypoxic tumor microenvironment is one of the important impediments for conventional cancer therapy. This study aimed to computationally identify hypoxia-related mRNA signatures in nine hypoxic-conditioned cancer cell lines and investigate their role during hypoxia. METHODS: Nine RNA-Seq expression data sets were retrieved from the Gene Expression Omnibus database. DEGs were identified in each cancer cell line. Then 23 common DEGs were selected by comparing the gene lists across the nine cancer cell lines...
December 12, 2022: Iranian Biomedical Journal
https://read.qxmd.com/read/36199792/identification-of-six-n7-methylguanosine-related-mirna-signatures-to-predict-the-overall-survival-and-immune-landscape-of-triple-negative-breast-cancer-through-in-silico-analysis
#3
JOURNAL ARTICLE
Jing Xu, Xiaoxia Cen, Yu Yao, Suo Zhao, Wei Li, Wei Zhang, Ming Qiu
Triple-negative breast cancer (TNBC) is a widely prevalent breast cancer, with a mortality rate of up to 25%. TNBC has a lower survival rate, and the significance of N7-methylguanosine (m7G) modification in TNBC remains unclear. Thus, this study is aimed at investigating m7G-related miRNAs in TNBC patients through in silico analysis. In our research, RNA sequencing and clinical data were obtained from The Cancer Genome Atlas (TCGA) database. The miRNAs targeting typical m7G modification regulators Methyltransferase-like 1 (METTL1) and WD repeat domain 4 (WDR4) were predicted on the TargetScan website...
2022: Journal of Oncology
https://read.qxmd.com/read/36180946/circulating-and-urinary-micrornas-profile-for-predicting-renal-recovery-from-severe-acute-kidney-injury
#4
JOURNAL ARTICLE
Thanawat Phulkerd, Tanat Lertussavavivat, Umaporn Limothai, Sadudee Peerapornratana, Win Kulvichit, Nuttha Lumlertgul, Kriang Tungsanga, Somchai Eiam-Ong, Yingyos Avihingsanon, Nattachai Srisawat
BACKGROUND: There is little known about the contribution of microRNAs (miRNAs) in the recovery from acute kidney injury (AKI). This study aimed to discover and validate miRNA profiles for predicting renal recovery from severe AKI. PATIENTS AND METHODS: A prospective observational study was conducted between June 2020 and January 2021. Urine and serum samples of participants with AKI stage 3 were collected from two groups: renal recovery and renal non-recovery. Transcriptomic analysis was performed using nCounter miRNA Expression Assay...
September 30, 2022: Journal of Intensive Care
https://read.qxmd.com/read/35477008/lncrna-tspear-as1-predicts-poor-prognosis-in-patients-with-hepatitis-b-virus-associated-hepatocellular-carcinoma-and-promotes-metastasis-via-mir-1915-5p
#5
JOURNAL ARTICLE
Yichuan Zhang, Fei Wang, Yu Wang
BACKGROUND: Hepatitis B virus-related hepatocellular carcinoma (HBV-HCC) will contribute to more than half of the liver deaths worldwide. This study aimed to investigate the prognostic value of TSPEAR-AS1 in HBV-HCC and its role in the HBV-HCC progression. METHODS: HBV-HCC tissue and adjacent non-cancerous tissues (ANT) were detected to figure out the expression level of TSPEAR-AS1 using real-time quantitative PCR. The relationship between TSPEAR-AS1 expression and each important clinical characteristic was evaluated...
April 24, 2022: Virus Research
https://read.qxmd.com/read/34987010/circrna-abcb10-promotes-gastric-cancer-progression-by-sponging-mir-1915-3p-to-upregulate-rac1
#6
JOURNAL ARTICLE
Junhui Liu, Guanglin Qiu, Haijiang Wang, Na Li, Xinhua Liao
BACKGROUND: Gastric cancer (GC) is a common malignant tumor of the digestive system. Increasing reports have demonstrated the crucial roles of circRNAs in tumorigenesis and progression of GC. METHODS: The relative expression of circ-ABCB10 in GC tissues and cell lines was detected by qRT-PCR. A series of in vitro assays and a xenograft model in vivo were applied to explore the function of circ-ABCB10 in GC cells. RESULTS: Circ-ABCB10 expression was upregulated in GC tissues and cell lines and positively correlated with poor survival of GC patients...
July 2022: Digestive and Liver Disease
https://read.qxmd.com/read/34904915/cellular-mir-150-5p-may-have-a-crucial-role-to-play-in-the-biology-of-sars-cov-2-infection-by-regulating-nsp10-gene
#7
JOURNAL ARTICLE
Shaw M Akula, Paul Bolin, Paul P Cook
The role for circulating miRNAs as biomarkers of the COVID-19 disease remains uncertain. We analysed the circulating miRNA profile in twelve COVID-19 patients with moderate-severe disease. This analysis was conducted by performing next generation sequencing (NGS) followed by real-time polymerase chain reaction (RT-qPCR). Compared with healthy controls, we detected significant changes in the circulating miRNA profile of COVID-19 patients. The miRNAs that were significantly altered in all the COVID-19 patients were miR-150-5p, miR-375, miR-122-5p, miR-494-3p, miR-3197, miR-4690-5p, miR-1915-3p, and miR-3652...
2022: RNA Biology
https://read.qxmd.com/read/34774019/microrna-1915-3p-inhibits-cell-migration-and-invasion-by-targeting-set-in-non-small-cell-lung-cancer
#8
JOURNAL ARTICLE
Hongli Pan, Zhenhua Pan, Fengjie Guo, Fanrong Meng, Lingling Zu, Yaguang Fan, Yang Li, Mengjie Li, Xinxin Du, Xiuwen Zhang, Yi Shao, Mingming Wei, Xuebing Li, Qinghua Zhou
BACKGROUND: MicroRNAs (miRNAs) have been reported to play significant roles in non-small-cell lung cancer (NSCLC). However, the roles of microRNA (miR)-1915-3p in NSCLC remain unclear. In this study, we aimed to explore the biological functions of miR-1915-3p in NSCLC. METHODS: The expression of miR-1915-3p and SET nuclear proto-oncogene (SET) in NSCLC tissues were examined by quantitative real-time PCR (qRT-PCR). Migratory and invasive abilities of lung cancer were tested by wound healing and transwell invasion assay...
November 13, 2021: BMC Cancer
https://read.qxmd.com/read/34680382/small-extracellular-vesicle-derived-micrornas-stratify-prostate-cancer-patients-according-to-gleason-score-race-and-associate-with-survival-of-african-american-and-caucasian-men
#9
JOURNAL ARTICLE
Hamdy E A Ali, Mohamed S A Gaballah, Rofaida Gaballa, Shahenda Mahgoub, Zeinab A Hassan, Eman A Toraih, Bettina F Drake, Zakaria Y Abd Elmageed
The utility of small extracellular vesicles (sEVs)-derived microRNAs (miRs) to segregate prostate cancer (PCa) patients according to tumor aggressiveness and ancestral background has not been fully investigated. Thus, we aimed to determine the diagnostic and prognostic utility of sEV-associated miRs in identifying aggressive PCa in African American (AA) and Caucasian (CA) men. Using a training cohort, miR profiling was performed on sEVs isolated from plasma of PCa patients. Top-ranked sEV-associated miRs were then validated in 150 plasma samples (75 AA and 75 CA) collected from two independent cohorts; NIH ( n = 90) and Washington University ( n = 60) cohorts...
October 19, 2021: Cancers
https://read.qxmd.com/read/34599680/evs-delivery-of-mir-1915-3p-improves-the-chemotherapeutic-efficacy-of-oxaliplatin-in-colorectal-cancer
#10
JOURNAL ARTICLE
Zhenhuan Xiao, Yun Liu, Qun Li, Qinyuan Liu, Yong Liu, Yan Luo, Songzhi Wei
PURPOSE: Oxaliplatin is a crucial component of the combinatorial chemotherapeutic standard of care for advanced colorectal cancer (CRC). Unfortunately, a serious barrier to effective oxaliplatin treatment is drug resistance due to epithelial-mesenchymal transitioning (EMT). Interestingly, stable oxaliplatin-resistant CRC cell lines show differential expression of miR-1915-3p; thus, this microRNA may represent a potential modifier of oxaliplatin resistance in CRC cells. METHODS: miR-1915-3p was over-expressed in oxaliplatin-resistant CRC cells and a non-tumorigenic intestinal cell line (FHC) via lentiviral transduction...
December 2021: Cancer Chemotherapy and Pharmacology
https://read.qxmd.com/read/34479063/diagnostic-predictability-of-mir-4535-and-mir-1915-5p-expression-in-amniotic-fluid-for-foetal-morbidity-of-infection
#11
JOURNAL ARTICLE
Kenichi Yoshikawa, Chihiro Kiyoshima, Toyofumi Hirakawa, Daichi Urushiyama, Satoshi Fukagawa, Daisuke Izuchi, Ayako Sanui, Masamitsu Kurakazu, Kohei Miyata, Makoto Nomiyama, Takashi Setoue, Shinichiro Nagamitsu, Kazuki Nabeshima, Kenichiro Hata, Shin'ichiro Yasunaga, Shingo Miyamoto
INTRODUCTION: Clinical prediction of foetal inflammatory response syndrome (FIRS) is highly necessary. We have previously reported that miR-4535 and miR-1915-5p are potential biomarkers for severe chorioamnionitis based on the results of microRNA array analysis. Therefore, we evaluated the relationship between foetal morbidity of infection and miR-4535, miR-1915-5p, interleukin (IL)-6, or 16S rDNA copy number levels in amniotic fluid from pregnant women with chorioamnionitis. METHODS: Amniotic fluid from 57 pregnant women with preterm premature membrane rupture or threatened premature labour were collected...
October 2021: Placenta
https://read.qxmd.com/read/34046191/micrornas-mir-4535-and-mir-1915-5p-in-amniotic-fluid-as-predictive-biomarkers-for-chorioamnionitis
#12
JOURNAL ARTICLE
Chihiro Kiyoshima, Naoto Shirasu, Daichi Urushiyama, Satoshi Fukagawa, Toyofumi Hirakawa, Kenichi Yoshikawa, Daisuke Izuchi, Kohei Miyata, Masamitsu Kurakazu, Fusanori Yotsumoto, Kenji Hiromatsu, Makoto Nomiyama, Ohta Eiji, Shinichi Hirose, Yoshitoshi Ogura, Tetsuya Hayashi, Kenichiro Hata, Kazuki Nabeshima, Shin'ichiro Yasunaga, Shingo Miyamoto
BACKGROUND: This study was performed to investigate the clinical significance of miR-4535 and miR-1915-5p in severe chorioamnionitis. MATERIALS & METHODS: Amniotic fluid samples from 37 patients with severe chorioamnionitis were subjected to miRNA array analysis and ddPCR™. Diagnostic values were assessed using the receiver operating characteristic curve. The patients were separated into three groups according to Blanc's criteria. RESULTS: The expression of miR-4535 and miR-1915-5p was significantly correlated with the copy number of 16S rDNA, had extremely high diagnostic accuracy for severe chorioamnionitis, and was linked to maternal and fetal inflammation...
February 15, 2021: Future Science OA
https://read.qxmd.com/read/33009394/platelet-derived-microparticles-enhance-megakaryocyte-differentiation-and-platelet-generation-via-mir-1915-3p
#13
JOURNAL ARTICLE
Mingyi Qu, Xiaojing Zou, Fang Fang, Shouye Wang, Lei Xu, Quan Zeng, Zeng Fan, Lin Chen, Wen Yue, Xiaoyan Xie, Xuetao Pei
Thrombosis leads to platelet activation and subsequent degradation; therefore, replenishment of platelets from hematopoietic stem/progenitor cells (HSPCs) is needed to maintain the physiological level of circulating platelets. Platelet-derived microparticles (PMPs) are protein- and RNA-containing vesicles released from activated platelets. We hypothesized that factors carried by PMPs might influence the production of platelets from HSPCs, in a positive feedback fashion. Here we show that, during mouse acute liver injury, the density of megakaryocyte in the bone marrow increases following an increase in circulating PMPs, but without thrombopoietin (TPO) upregulation...
October 2, 2020: Nature Communications
https://read.qxmd.com/read/32871836/serum-exosomal-mirna-1915-3p-is-correlated-with-disease-activity-of-korean-rheumatoid-arthritis
#14
JOURNAL ARTICLE
Mi-Kyoung Lim, Jihyung Yoo, Dong-Hyuk Sheen, Chunhwa Ihm, Sang Kwang Lee, Soon Ae Kim
BACKGROUND/AIM: It has been found that microRNAs (miRNA) affect rheumatoid arthritis (RA) pathophysiology. This study aimed to identify novel serum exosomal miRNAs related to RA disease activity in patients with an inadequate treatment response. PATIENTS AND METHODS: The sample population comprised clinical remission (CR) and non-clinical remission (non-CR) groups of RA patients. To identify potent miRNA markers for RA disease activity, miRNA array and qPCR were performed after patient serum exosomes preparation...
September 2020: In Vivo
https://read.qxmd.com/read/31808923/assessment-of-the-diagnostic-utility-of-serum-microrna-classification-in-patients-with-diffuse-glioma
#15
JOURNAL ARTICLE
Makoto Ohno, Juntaro Matsuzaki, Junpei Kawauchi, Yoshiaki Aoki, Junichiro Miura, Satoko Takizawa, Ken Kato, Hiromi Sakamoto, Yuko Matsushita, Masamichi Takahashi, Yasuji Miyakita, Koichi Ichimura, Yoshitaka Narita, Takahiro Ochiya
Importance: A blood-based screening tool for detecting diffuse glioma is necessary to improve clinical outcomes. Objectives: To establish models using serum microRNAs to distinguish patients with diffuse glioma from control individuals without cancer (the Glioma Index) and to differentiate glioblastoma (GBM), primary central nervous system lymphoma (PCNSL), and metastatic brain tumors (the 3-Tumor Index). Design, Setting, and Participants: This retrospective, case-control diagnostic study included 157 patients with diffuse glioma and 109 patients with central nervous system (CNS) diseases other than diffuse glioma diagnosed from August 1, 2008, through May 1, 2016, and 314 sex- and age-matched controls without cancer...
December 2, 2019: JAMA Network Open
https://read.qxmd.com/read/31675148/exosomal-mirnas-as-potential-biomarkers-for-acute-myocardial-infarction
#16
JOURNAL ARTICLE
Jia Su, Jiyi Li, Qinglin Yu, Jingqiao Wang, Xiaojing Li, Jin Yang, Jin Xu, Yahui Liu, Zhifeng Xu, Lindan Ji, Fengying Yin, Xiaomin Chen
microRNAs (miRNAs) can be used as biomarkers for acute myocardial infarction (AMI). However, few reports have focused on the value of exosomal miRNAs in the mechanism of the pathophysiological process from stable coronary artery disease (SCAD) to AMI. Exosomes were isolated via ultracentrifugation after serum samples were collected. The exosomes were then identified by transmission electron microscopy, western blotting, and nanoparticle tracking analysis. The differential expression of miRNAs in exosomes from six AMI and six matching SCAD patients was screened using the Agilent Human miRNA Microarray Kit...
March 2020: IUBMB Life
https://read.qxmd.com/read/30787377/prenatal-circulating-microrna-signatures-of-foetal-down-syndrome
#17
JOURNAL ARTICLE
Monika Zbucka-Kretowska, Magdalena Niemira, Magdalena Paczkowska-Abdulsalam, Agnieszka Bielska, Anna Szalkowska, Ewa Parfieniuk, Michal Ciborowski, Slawomir Wolczynski, Adam Kretowski
The altered expression pattern of miRNAs might potentially reflect anomalies related to foetal chromosomal aberrations. The aim of the study was to determine the expression level of miRNAs in plasma of pregnant women with foetal Down syndrome (DS). Out of 198 amniocentesis performed at 15-18 weeks of gestation, within a group of 12 patients with foetal DS and 12 patients with uncomplicated pregnancies, who delivered healthy newborns at term, we examined the expression level of 800 miRNAs using the NanoString technology...
February 20, 2019: Scientific Reports
https://read.qxmd.com/read/30605755/integrated-analysis-of-microrna-mrna-expression-in-a549-cells-infected-with-influenza-a-viruses-iavs-from-different-host-species
#18
JOURNAL ARTICLE
Jie Gao, Lingxi Gao, Rui Li, Zhenping Lai, Zengfeng Zhang, Xiaohui Fan
Although several miRNAs have been demonstrated to be involved in the influenza virus replication cycle, the identification of miRNAs and mRNAs that are expressed in A549 cells infected with influenza A viruses (IAVs) from different host species has remained poorly studied. To investigate the molecular mechanisms associated with the differential expression of miRNAs during influenza A virus infection, we performed global miRNA and mRNA expression profiling in A549 cells infected with human-origin seasonal influenza A virus H3N2 (Human_Br07), swine-origin influenza A virus H1N1 (SW_3861) or avian-origin influenza A virus H3N2 (AVI_9990)...
April 2, 2019: Virus Research
https://read.qxmd.com/read/30554489/up-regulation-of-mir-1915-inhibits-proliferation-invasion-and-migration-of-helicobacter-pylori-infected-gastric-cancer-cells-via-targeting-rage
#19
JOURNAL ARTICLE
Xin Cai Xu, Wen Bin Zhang, Chun Xing Li, Hua Gao, Qi Pei, Bo Wei Cao, Tie Han He
PURPOSE: Helicobacter pylori (HP) -infected gastric cancer (GC) is known to be a fatal malignant tumor, but the molecular mechanisms underlying its proliferation, invasion, and migration remain far from being completely understood. Our aim in this study was to explore miR-1915 expression and its molecular mechanisms in regulating proliferation, invasion, and migration of HP -infected GC cells. MATERIALS AND METHODS: Quantitative real-time PCR and western blot analysis were performed to determine miR-1915 and receptor for advanced glycation end product (RAGE) expression in HP -infected GC tissues and gastritis tissues, as well as human gastric mucosal cell line GES-1 and human GC cell lines SGC-7901 and MKN45...
January 2019: Yonsei Medical Journal
https://read.qxmd.com/read/30258285/key-elements-involved-in-epstein-barr-virus-associated-gastric-cancer-and-their-network-regulation
#20
JOURNAL ARTICLE
Jing-Jing Jing, Ze-Yang Wang, Hao Li, Li-Ping Sun, Yuan Yuan
BACKGROUND: The molecular mechanism of Epstein-Barr virus (EBV)-associated gastric cancer (EBVaGC) remains elusive. A collection of molecular regulators including transcription factor and noncoding RNA (ncRNAs) may affect the carcinogenesis of EBVaGC by regulating the expression and function of key genes. In this study, integration of multi-level expression data and bioinformatics approach was used to identify key elements and their interactions involved in mechanism of EBVaGC and their network regulation...
2018: Cancer Cell International
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