keyword
https://read.qxmd.com/read/38533056/molecular-identification-of-different-toxinogenic-strains-of-clostridium-perfringens-and-histo-pathological-observations-of-camels-died-of-per-acute-entero-toxaemia
#1
JOURNAL ARTICLE
Hafiz Muhammad Ali, Shujaat Hussain, Muhammad Zishan Ahmad, Abu Baker Siddique, Sultan Ali, Mudassar Mohiuddin, Muhammad Ehsan, Muhammad Nadeem, Abdul Qayyum, Riaz Hussain, Iahtasham Khan, Dunia A Al Farraj, Enshad Alzaidi
Enterotoxaemia is a severe disease caused by Clostridium perfringens and render high mortality and huge economic losses in livestock. However, scanty information and only few cases are reported about the presence and patho-physiology of enterotoxaemia in camels. The bacterium induces per-acute death in animals due to rapid production of different lethal toxins. The necropsy of camels (per-acute = 15, acute = 3) was conducted at 18 outbreaks of enterotoxaemia in camels in the desert area of Bahawalpur region...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38526222/the-adaptor-protein-ecap-the-co-repressor-leunig-and-the-transcription-factor-beh3-interact-and-regulate-microsporocyte-generation-in-arabidopsis
#2
JOURNAL ARTICLE
Lei Shi, Changjiang Li, Gaofeng Lv, Xing Li, Wutao Feng, Yujing Bi, Wenhui Wang, Youqun Wang, Lei Zhu, Wenqiang Tang, Ying Fu
Histo-specification and morphogenesis of anthers during development in Arabidopsis (Arabidopsis thaliana) are well understood. However, the regulatory mechanism of microsporocyte generation at the pre-meiotic stage remains unclear, especially how archesporial cells are specified and differentiate into two cell lineages with distinct developmental fates. SPOROCYTELESS (SPL) is a key reproductive gene that is activated during early anther development and remains active. In this study, we demonstrated that the EAR motif-containing adaptor protein (ECAP) interacts with the Gro/Tup1 family co-repressor LEUNIG (LUG) and the BES1/BZR1 HOMOLOG3 (BEH3) transcription factor to form a transcription activator complex, epigenetically regulating SPL transcription...
March 25, 2024: Plant Cell
https://read.qxmd.com/read/38519928/enhancing-effect-of-oregano-essential-oil-and-bacillus-subtilis-on-broiler-immune-function-intestinal-morphology-and-growth-performance
#3
JOURNAL ARTICLE
Yehia El-Sayed, Waleed Khalil, Nada Fayez, Abdel-Fattah Mohamed Abdel-Fattah
The present study evaluated the effect of two categories of feed additives on chicken performance through immunological and intestinal histo-morphometric measurements. A total of 150 one-day-old male broiler chicks (Cobb) were randomly assigned to three groups. Group I received a non-supplemented basal diet. While groups II and III were treated with a basal diet supplemented with oregano essential oil (OEO) and Bacillus subtilis, respectively, in water for 28 days. Blood samples were taken at 6, 18 and 28 days for hematological analysis, phagocytosis, lymphocyte proliferation and measuring antibody responses...
March 22, 2024: BMC Veterinary Research
https://read.qxmd.com/read/38506097/histological-outcomes-and-jak-stat-signalling-in-ulcerative-colitis-patients-treated-with-tofacitinib
#4
JOURNAL ARTICLE
Sara van Gennep, Ivan C N Fung, Djuna C de Jong, Rishand K Ramkisoen, Esmé Clasquin, Jitteke de Jong, Leonie C S de Vries, Wouter J de Jonge, Krisztina B Gecse, Mark Löwenberg, John C Woolcott, Aart Mookhoek, Geert R D'Haens
BACKGROUND AND AIMS: Histological outcomes and JAK-STAT signaling were assessed in a prospective ulcerative colitis (UC) patient cohort after 8 weeks treatment with tofacitinib, an oral Janus kinase (JAK) inhibitor. METHODS: Forty UC patients received tofacitinib 10 mg twice daily for 8 weeks. Treatment response was defined as histo-endoscopic mucosal improvement (HEMI). Histological remission was defined as a Robarts Histopathology Index (RHI) ≤3 points and histological response as 50% decrease in RHI...
March 20, 2024: Journal of Crohn's & Colitis
https://read.qxmd.com/read/38505130/evaluation-of-long-term-effects-of-nickel-and-benzo-a-anthracene-contaminated-diets-in-rats-kidney-mimicking-human-exposure-from-food
#5
JOURNAL ARTICLE
Peter Ifeoluwa Adegbola, Abiodun Bukunmi Aborisade, Temitope Deborah Olaniyi, Adewale Adetutu
OBJECTIVES: Accumulative effects of heavy metals and polycyclic aromatic hydrocarbon could result in various toxicities. This study evaluated the effects of long-term exposure to low doses of nickel and benzo [a] anthracene on the kidney of rats, simulating human exposure through food. METHODS: Thirty-six (36) Male rats weighing between 80-100 g were assigned into six groups of 6 animals each; Group A (normal), Group B1 and B2 (fed nickel contaminated feed for 12 and 24 weeks), Group C1 and C2 (fed benzo [a] anthracene contaminated feed for 12 and 24 weeks)...
2024: International Journal of Biochemistry and Molecular Biology
https://read.qxmd.com/read/38494467/a-radio-anatomical-study-of-median-arcuate-ligament-syndrome-unveiling-the-morphology-and-morphometry-of-median-arcuate-ligament-celiac-trunk-and-superior-mesenteric-artery
#6
JOURNAL ARTICLE
Arthi Ganapathy, Sudipta Mohakud, Sipra Rout, Praisy Joy, Alamelu Alagappan, Aarthi Manokaran
PURPOSE: In the context of medical literature, a significant lacuna exists in understanding median arcuate ligament syndrome (MALS). While clinical aspects are well documented, literature lacks a robust exploration of the anatomical relationship between the celiac trunk and the median arcuate ligament (MAL). METHODS: Morphometric parameters, including the vertebral level of MAL origin, MAL thickness, celiac trunk (CeT) origin level, diameter, and distances between CeT/Superior Mesenteric Artery (SMA) and the MAL center were observed on 250 CT angiograms...
March 17, 2024: Abdominal Radiology
https://read.qxmd.com/read/38482673/biomarker-characterization-in-endometrial-cancer-in-europe-first-survey-data-analysis-from-69-pathological-academic-and-hospital-labs
#7
JOURNAL ARTICLE
Angela Santoro, Emma Bragantini, Francesca Castiglione, Raji Ganesan, Xavier Matias-Guiu, Milo Frattini, Valerio Gallotta, Pablo Garcia, Yatish Pattni, Julia Tsiampali-Laprell, Brigitte Bisaro, Mattia Barbareschi, Gian Franco Zannoni
INTRODUCTION: Endometrial carcinoma (EC) is the commonest gynecological cancer affecting women in Western populations. To predict patient risk, the 2020 edition of the World Health Organization (WHO) Classification of Tumors of the Female Genital Tract stressed the importance of integrated histo-molecular classification of the disease. This survey analysis poses attention on the most frequently used immunohistochemical and molecular markers adopted in daily categorization of ECs in European laboratories...
February 2024: Pathologica
https://read.qxmd.com/read/38478226/adult-stem-cells-freezing-processes-and-cryopreservation-protocols
#8
JOURNAL ARTICLE
Mohan Kumar Dey, Ram V Devireddy
The development of simple but effective storage protocols for adult stem cells will greatly enhance their use and utility in tissue-engineering applications. Cryopreservation has shown the most promise but is a fairly complex process, necessitating the use of chemicals called cryoprotective agents (CPAs), freezing equipment, and obviously, storage in liquid nitrogen. The purpose of this chapter is to present a general overview of cryopreservation storage techniques and the optimal protocols/results obtained in our laboratory for long-term storage of adult stem cells using freezing storage...
2024: Methods in Molecular Biology
https://read.qxmd.com/read/38465321/effect-of-nanocurcumin-on-fertility-in-murine-model-of-polycystic-ovary-syndrome
#9
JOURNAL ARTICLE
Zahra Aaly-Gharibeh, Mohammadreza Hosseinchi, Ali Shalizar-Jalali
The precise pathophysiology of polycystic ovary syndrome (PCOS) is not well-founded. In an attempt to fill this gap, the current study was executed to probe the effect of nanocurcumin (NCC) on ovarian tissue, in vitro fertilization (IVF) and pre-implantation embryo development in a mouse model of PCOS. Fifty adult female mice were randomly categorized into five equal groups including non-treated control and PCOS (receiving 0.20 mg estradiol valerate (EV) intra-peritoneally once a day for 21 days) as well as NCC12...
2024: Veterinary Research Forum
https://read.qxmd.com/read/38463890/the-variation-of-antigenic-and-histo-blood-group-binding-sites-synergistically-drive-the-evolution-among-chronologically-emerging-gii-4-noroviruses
#10
JOURNAL ARTICLE
Xiaojing Hong, Liang Xue, Yingwen Cao, Ruiquan Xu, Jingmin Wang, Junshan Gao, Shuidi Miao, Yueting Jiang, Xiaoxia Kou
Norovirus, commonly found on shellfish and vegetables, is a foodborne virus with GII.4 as the dominant genotype responsible for widespread outbreaks since 1995. Continuous variation of major capsid protein VP1 can lead to changes in the immunogenicity and host receptor binding ability of norovirus, which is an important evolutionary mechanism. Therefore, analyzing the immunogenicity of VP1 and its binding ability to various HBGAs in GII.4 variants could improve our understanding of the persistent prevalence of GII...
March 15, 2024: Heliyon
https://read.qxmd.com/read/38454623/texture-analysis-of-18f-fdg-pet-ct-and-cect-prediction-of-refractoriness-of-hodgkin-lymphoma-with-mediastinal-bulk-involvement
#11
JOURNAL ARTICLE
Elisabetta M Abenavoli, Flavia Linguanti, Matilde Anichini, Vittorio Miele, Francesco Mungai, Marianna Palazzo, Luca Nassi, Benedetta Puccini, Ilaria Romano, Benedetta Sordi, Roberto Sciagrà, Gabriele Simontacchi, Alessandro M Vannucchi, Valentina Berti
To recognize patients at high risk of refractory disease, the identification of novel prognostic parameters improving stratification of newly diagnosed Hodgkin Lymphoma (HL) is still needed. This study investigates the potential value of metabolic and texture features, extracted from baseline 18F-FDG Positron Emission Tomography/Computed Tomography (PET) and Contrast-Enhanced Computed Tomography scan (CECT), together with clinical data, in predicting first-line therapy refractoriness (R) of classical HL (cHL) with mediastinal bulk involvement...
March 2024: Hematological Oncology
https://read.qxmd.com/read/38449804/liver-boosting-potential-chicory-compound-mediated-silver-nanoparticles-for-hepatoprotection-biochemical-and-histopathological-insights
#12
JOURNAL ARTICLE
Ayesha Siddiqa, Rahmatullah Qureshi, Naveed Iqbal Raja, Imtiaz Ahmed Khan, Muhammad Zishan Ahmad, Shaista Rafique, Amir Ali, Ajaz Ahmad, Prashant Kaushik
Background: Liver disease is a serious health concern in today's world, posing a challenge to both healthcare providers and pharmaceutical companies. Most synthetic drugs and chemicals cause liver damage accounting for approximately 10% of acute hepatitis and 50% of acute liver failure. Purpose: The present study aimed to evaluate the hepato-protective activity of an extract of chicory formulation assisted by silver nanoparticles against carbon tetra chloride (CCl4 )-induced hepatic damage in rat's liver. Methods: Rats of the Wistar strain (Rattus norvegicus) were used to test the in vivo hepato-protective efficacy at various doses...
2024: Frontiers in Pharmacology
https://read.qxmd.com/read/38440621/the-significance-of-systemic-inflammatory-markers-in-prognosis-of-head-and-neck-squamous-cell-cancers
#13
JOURNAL ARTICLE
Bhushan Lal, Diksha Thakur, Payal Gupta, Madhuri Dadwal, Mahender Singh
This present study aimed to assess the predictive significance of two systemic inflammatory markers, the neutrophilic to lymphocytic ratio (NLR) and platelet to lymphocytic ratio (PLR), in evaluating the prognosis of individuals. The research involved 47 patients diagnosed with head and neck squamous cell carcinoma, all of whom were histo-pathologically confirmed and aged over 18 years. The patients were monitored every 6 months for a period of 18 months. The average age of the study participants was 57...
February 2024: Indian Journal of Otolaryngology and Head and Neck Surgery
https://read.qxmd.com/read/38431316/examining-the-efficiency-of-porcine-gastric-mucin-coated-magnetic-beads-in-extraction-of-noroviruses-from-frozen-berries
#14
JOURNAL ARTICLE
Daniel Plante, Julio Alexander Bran Barrera, Maude Lord, Jennifer Harlow, Irène Iugovaz, Neda Nasheri
Human norovirus is the leading cause of foodborne gastroenteritis worldwide. Due to the low infectious dose of noroviruses, sensitive methodologies are required to detect and characterize small numbers of viral particles that are found in contaminated foods. The ISO 15216 method, which is internationally recognized for detection of foodborne viruses from high-risk food commodities, is based on viral precipitation, followed by RNA extraction and identification of the viral genome by RT-PCR. Although the ISO 15216 method is efficient, it is time consuming and tedious, does not report on the viral infectivity, and is sensitive to the presence of RT-PCR inhibitors...
June 2024: Food Microbiology
https://read.qxmd.com/read/38428737/molecular-and-clinical-portrait-of-her2-low-invasive-lobular-carcinomas
#15
JOURNAL ARTICLE
Lounes Djerroudi, Ahmad El Sabeh-Ayoun, Camille Benoist, Gaelle Pierron, Julien Masliah-Planchon, Laetitia Fuhrmann, Yann Kieffer, Matthieu Carton, Toulsie Ramtohul, Celine Callens, Victor Renault, François-Clément Bidard, Fatima Mechta-Grigoriou, Anne Vincent-Salomon
Invasive lobular carcinomas (ILCs) have a low frequency of ERBB2 amplification, therefore restricting the use of conventional anti-HER2 therapies for this histological special type. Conversely, ILCs with low HER2 overexpression may represent a broader target for the use of emerging anti-body drug conjugate therapies targeting HER2, since these treatments have proven effective in HER2-low breast cancers. Very scarce data about HER2-low ILCs have been so far published, although these tumors could have different prevalence and histo-molecular specificities compared to invasive breast carcinoma of no special type (IBC-NST)...
February 28, 2024: Modern Pathology
https://read.qxmd.com/read/38420243/pseudotumor-cysticercosis-of-the-flexor-digitorum-profundus-mimicking-volkmanns-contracture
#16
Dhruv A Shah, Ashok M Ghodke, Shahish S Surme, Laksh A Agrawal, Ameya H Velankar
INTRODUCTION: Cysticercosis leads to a cyst formation known to occur due to the Taenia solium parasite. Patients normally present with seeding and formation of the cysts in the central nervous system (CNS) as neurocysticercosis (NCC). Intramuscular cysts are rare presentations and are mostly incidental findings in NCC patients. We present a rare case of a rapidly progressing isolated cysticercosis of the flexor digitorum profundus (FDP) muscle leading to a pseudotumor and presenting with pseudo-Volkmanns contracture and a positive Volkmanns sign...
February 2024: Journal of Orthopaedic Case Reports
https://read.qxmd.com/read/38406842/beating-the-heart-failure-odds-long-term-survival-after-myocardial-ischemia-in-juvenile-rainbow-trout
#17
JOURNAL ARTICLE
Lucas A Zena, Andreas Tommy Ekström, Daniel Morgenroth, Tristan McArley, Michael Axelsson, Henrik Sundh, Anders Palmquist, Ida B Johansen, Albin Gräns, Erik Sandblom
Salmonid fish include some of the most valued cultured fish species worldwide. Unlike most other fish, the hearts of salmonids, including Atlantic salmon and rainbow trout, have a well-developed coronary circulation. Consequently, their hearts' reliance on oxygenation through coronary arteries leaves them prone to coronary lesions, believed to precipitate myocardial ischemia. Here, we mimicked such coronary lesions by subjecting groups of juvenile rainbow trout to coronary ligation, assessing histo-morphological myocardial changes associated with ischemia and scarring in the context of cardiac arrhythmias using electrocardiography (ECG)...
February 26, 2024: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://read.qxmd.com/read/38367601/hepatic-granulomas-following-liver-transplantation-a-retrospective-survey-and-analysis-of-possible-microbiological-etiology
#18
JOURNAL ARTICLE
Anisoara Iordache, Kurt Fuursted, Charlotte Vestrup Rift, Allan Rasmussen, Gro Linno Willemoe, Jane Preuss Hasselby
BACKGROUND: Liver granulomas have always been a diagnostic challenge for pathologists. They have been described in up to 15% of liver biopsies and can also be seen in liver allograft biopsy specimens, but there is a paucity of information regarding the prevalence and associated etiologic factors of granulomas in liver transplanted patients. The aim of this study is to shed light on the etiology of liver granulomas. METHODS: Liver biopsies from liver transplanted patients, in the period from 01...
February 9, 2024: Pathology, Research and Practice
https://read.qxmd.com/read/38353537/strain-specific-requirements-of-susceptibility-to-rhesus-enteric-calicivirus-infection
#19
JOURNAL ARTICLE
Tibor Farkas, Vinod Srivastava
Recently, we identified the coxsackie and adenovirus receptor (CAR) as the entry receptor for rhesus enteric calicivirus (ReCV) isolate FT285 and demonstrated that co-expression of the CAR and the type B histo-blood group antigen (HBGA) is required to convert the resistant CHO cell line susceptible to infection. To address whether the CAR is also the functional entry receptor for other ReCV isolates and the requirement for specific HBGAs or other glycans, here we used a panel of recombinant CHO cell lines expressing the CAR and the type A, B, or H HBGAs alone or in combination...
February 14, 2024: Journal of Virology
https://read.qxmd.com/read/38353323/effect-of-in-ovo-inoculation-of-betaine-on-hatchability-serum-antioxidant-levels-muscle-gene-expression-and-intestinal-development-of-broiler-chicks
#20
JOURNAL ARTICLE
Saad N El-Shater, Khaled Abo-El-Sooud, Ayman Tolba, Mahmoud Gamal, Mohamed A Awad, Moataz Ibrahim, Mahmoud Tayeh, Gamal A Swielim
This study investigated the effects of in-ovo inoculation of betaine on hatchability, hatching weight, and intestinal development, as well as serum and expression levels of some antioxidants in the posthatched chicks. A total of 350 fertile eggs of Hubbard efficiency plus breeder's flock were incubated at normal incubation temperature (37.5°C) and randomly assembled into 3 groups with 4 replicates, and 25 eggs per each. The experimental groups were allocated as noninjected control group (CN), diluent-injected group (CP, 0...
February 14, 2024: Journal of Animal Physiology and Animal Nutrition
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