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Blastocystis sp

Mehmet Burak Selek, Bayhan Bektöre, Ergenekon Karagöz, Orhan Baylan, Mustafa Özyurt
OBJECTIVE: Parasitic diseases are among the major public health issues worldwide. A number of tests are available for diagnosis, but the sentivity and specifity of these tests are assumed to be insufficient. Nevertheless, the most common diagnostic method is microscopic examination. In this study, we aimed to introduce the distribution of parasites detected in stool samples of patients admitted to our laboratory on the basis of parameters such as, age, and gender during a 3-year period between 2012 and 2014...
September 2016: Türkiye Parazitolojii Dergisi
Kambiz Yaghoobi, Bahador Sarkari, Majid Mansouri, Mohammad Hossein Motazedian
AIM: Wild boars, Sus scrofa, are potential reservoirs of many zoonotic diseases, and there are a possibility of transmission of the zoonotic diseases from these animals to humans and also domestic animals. This study aimed to evaluate the protozoan contamination of wild boars in the Persian Gulf's coastal area (Bushehr Province), southwestern Iran. MATERIALS AND METHODS: A total of 25 crossbred boars were collected during a course of vertebrate pest control in Bushehr province, in 2013...
October 2016: Veterinary World
Davin Edric V. Adao, Ace O. Dela Serna, Maria Luz B. Belleza, Nicole R. Bolo, Windell L. Rivera
Blastocystis sp. is a commonly reported enteric protistan parasite in faecal specimens with a worldwide distribution afflicting both humans and a wide range of animals. The aim of this study is to characterize the subtypes (STs) of Blastocystis sp. isolates from asymptomatic individuals in an urban community in Pateros, Metro Manila, Philippines. The 600-bp small subunit ribosomal RNA (SSU rRNA) barcoding region of Blastocystis sp. isolates was amplified and sequenced using the primers RD5 and BhRDr. Subtypes were identified by uploading the sequences onto the Basic Local Alignment and Search Tool (BLAST) websites, the Blastocystis Subtype (18S) and Sequence Typing (MLST) Database and by construction of a phylogenetic tree...
October 1, 2016: Annals of Parasitology
Samseh Abdullah Noradilah, Ii Li Lee, Tengku Shahrul Anuar, Fatmah Md Salleh, Siti Nor Azreen Abdul Manap, Noor Shazleen Husnie Mohd Mohtar, Syed Muhamad Azrul, Wan Omar Abdullah, Norhayati Moktar
In the tropics, there are too few studies on isolation of Blastocystis sp. subtypes from water sources; in addition, there is also an absence of reported studies on the occurrence of Blastocystis sp. subtypes in water during different seasons. Therefore, this study was aimed to determine the occurrence of Blastocystis sp. subtypes in river water and other water sources that drained aboriginal vicinity of highly endemic intestinal parasitic infections during wet and dry seasons. Water samples were collected from six sampling points of Sungai Krau (K1-K6) and a point at Sungai Lompat (K7) and other water sources around the aboriginal villages...
2016: PeerJ
Fernando Salvador, Elena Sulleiro, Adrián Sánchez-Montalvá, Carmen Alonso, Javier Santos, Isabel Fuentes, Israel Molina
BACKGROUND: Blastocystis spp. are among the most frequently observed intestinal parasites in humans. Despite the discovery of Blastocystis approximately 100 years ago, limited information is available regarding its pathogenesis, genetic diversity, and available treatment options. The aim of this study was to describe the epidemiological and clinical characteristics of patients with Blastocystis sp. infections diagnosed at Vall d'Hebron University Hospital (Barcelona, Spain). METHODS: A retrospective observational study was performed which included all adult patients who attended Vall d'Hebron University Hospital from February 2009 to March 2014 that had Blastocystis sp...
October 14, 2016: Parasites & Vectors
Liliana Simões-Silva, Inês Correia, Joana Barbosa, Carla Santos-Araujo, Maria João Sousa, Manuel Pestana, Isabel Soares-Silva, Benedita Sampaio-Maia
Currently, chronic kidney disease (CKD) is a global health problem. Considering the impaired immunity of CKD patients, the relevance of infection in peritoneal dialysis (PD), and the increased prevalence of parasites in CKD patients, protozoa colonization was evaluated in PD effluent from CKD patients undergoing PD. Overnight PD effluent was obtained from 49 asymptomatic stable PD patients. Protozoa analysis was performed microscopically by searching cysts and trophozoites in direct wet mount of PD effluent and after staining smears...
September 2016: Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis
Dima El Safadi, Amandine Cian, Céline Nourrisson, Bruno Pereira, Christelle Morelle, Patrick Bastien, Anne-Pauline Bellanger, Françoise Botterel, Ermanno Candolfi, Guillaume Desoubeaux, Laurence Lachaud, Florent Morio, Christelle Pomares, Meja Rabodonirina, Ivan Wawrzyniak, Frédéric Delbac, Nausicaa Gantois, Gabriela Certad, Laurence Delhaes, Philippe Poirier, Eric Viscogliosi
BACKGROUND: Blastocystis sp. is the most common intestinal parasite of humans. Despite its potential public health impact, epidemiological data regarding the prevalence and molecular subtype distribution of Blastocystis sp. in Europe are rarely reported. Therefore, the first multi-center epidemiological survey performed in Europe was conducted in France to diagnose and subtype Blastocystis sp. and to identify risk factors for infection. METHODS: Stool samples from 788 patients were collected either in summer or winter in 11 hospitals throughout France together with patient data...
2016: BMC Infectious Diseases
Marawan A Abu-Madi, Jerzy M Behnke, Sonia Boughattas, Asma Al-Thani, Sanjay H Doiphode
BACKGROUND: The World Health Organization estimates that about 3.5 billion people worldwide are affected by intestinal parasitic infections. Reports have already emphasized the role of immigrants in outbreaks of parasitic diseases in industrialized countries. With the mass influx of immigrants to Qatar, patent intestinal parasitic infections have been observed. Herein, the prevalence of intestinal protozoan infections was analysed in 29,286 records of subjects referred for stool examination at the Hamad Medical Corporation over the course of a decade (2005 to 2014, inclusive)...
2016: BMC Infectious Diseases
Isaac Villegas-Gómez, Fernando Martínez-Hernández, Alejandro Urrea-Quezada, Mariana González-Díaz, María Durazo, Jesús Hernández, Guadalupe Erendira Orozco-Mosqueda, Guiehdani Villalobos, Pablo Maravilla, Olivia Valenzuela
Blastocystis sp. is an anaerobic intestinal microorganism commonly identified in the feces of several animals, including humans. Blastocystis exhibits high genetic polymorphism and at least 17 subtypes (ST) have been identified; ST1-ST3 are frequently found in the Americas. Furthermore, in vitro assays have shown that temperature and humidity can affect the viability of Blastocystis cysts. In this study, we describe the genetic variability and genetic differentiation among and within Blastocystis STs in adults and children from the cities of Hermosillo and Morelia cities, which represent arid and humid subtropical climatic regions of México, respectively...
October 2016: Infection, Genetics and Evolution
Devi Balkrishnan Dhurga, Kumar Suresh, Tian Chye Tan
The role and function of the granular life cycle stage in Blastocystis sp, remains uncertain despite suggestions being made that the granules are metabolic, reproductive and lipid in nature. This present study aims to understand granular formation by triggering apoptosis in Blastocystis sp. by treating them with metronidazole (MTZ). Blastocystis sp.cultures of 4 sub-types namely 1, 2, 3 and 5 when treated with 0.01 and 0.0001 mg/ml of metronidazole (MTZ) respectively showed many of the parasites to be both viable and apoptotic (VA)...
2016: PloS One
Alexandra M Goe, Darryl J Heard, J Roger Easley, Amy L Weeden, April L Childress, James F X Wellehan
A hand-raised, 5-mo-old, intact male Brazilian porcupine (Coendou prehensilis) was evaluated for chronic diarrhea, failure to thrive, and anorexia. On presentation the porcupette was dull, dehydrated, and passing yellow, malodourous, watery diarrhea. Cytologic examination of feces revealed a large number of organisms, morphologically consistent with Blastocystis. Blastocystis polymerase chain reaction (PCR) performed on feces was positive. Direct sequencing on two sequential samples confirmed the presence of Blastocystis ratti and a novel Blastocystis sequence...
June 2016: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
Shifra Ken Dror, Elsa Pavlotzky, Mira Barak
Infectious gastroenteritis is a global health problem associated with high morbidity and mortality rates. Rapid and accurate diagnosis is crucial to allow appropriate and timely treatment. Current laboratory stool testing has a long turnaround time (TAT) and demands highly qualified personnel and multiple techniques. The need for high throughput and the number of possible enteric pathogens compels the implementation of a molecular approach which uses multiplex technology, without compromising performance requirements...
2016: PloS One
Reena Leeba Richard, Init Ithoi, Mohamad Azlan Abd Majid, Wan Yusoff Wan Sulaiman, Tian Chye Tan, Veeranoot Nissapatorn, Yvonne Ai Lian Lim
The occurrence of waterborne parasites coupled with water parameters at various processing sites of two drinking water treatment plants (A and B) and seven distribution system (DS) sites in Sarawak, Malaysia were studied. Ten liters of water underwent immunomagnetic separation (IMS) technique to detect the presence of Giardia and Cryptosporidium (oo)cysts. The remaining supernatant was used to detect other parasites whilst 50 mL of water sample was each used in the detection of free-living amoebae and fecal coliforms...
2016: International Journal of Environmental Research and Public Health
Joakim Forsell, Margareta Granlund, Linn Samuelsson, Satu Koskiniemi, Helén Edebro, Birgitta Evengård
BACKGROUND: Blastocystis is a common intestinal parasite with worldwide distribution but the distribution of Blastocystis and its subtypes in East Africa is largely unknown. In this study, we investigate the distribution of Blastocystis subtypes in Zanzibar, Tanzania and report the prevalence of intestinal parasites using both molecular methods and microscopy. METHODS: Stool samples were collected from both diarrhoeic and non-diarrhoeic outpatients in Zanzibar. In addition to microscopy, real-time PCR for Blastocystis, Entamoeba histolytica and E...
2016: Parasites & Vectors
Hamed Kiani, Ali Haghighi, Ali Rostami, Eznollah Azargashb, Seyyed Javad Seyyed Tabaei, Abbas Solgi, Nozhat Zebardast
We studied the prevalence of intestinal parasites (IPs), their risk factors and associated symptoms among patients with gastrointestinal disorders. A total of 1,301 participants aged 22 days-90 years were enrolled in this study. We used a structured questionnaire to obtain socio-demographic and stool examination to investigate intestinal parasite infections. Data analysis was performed using SPSS16. The overall prevalence of intestinal parasites (IPs) was 32.2% (419/1,301). Three hundred and fifty nine cases/1,301 (27...
2016: Revista do Instituto de Medicina Tropical de São Paulo
Gaby S Gil, Shobhana Chaudhari, Ahmed Shady, Ana Caballes, Joe Hong
We report an unusual case of severe diarrhea related to Blastocystis sp. infection in a patient with end stage renal disease on hemodialysis. The patient was admitted due to profuse diarrhea associated with fever and leukocytosis. Pertinent stool work-up such as leukocytes in stool, stool culture, clostridium difficile toxin B PCR, and serology for hepatitis A, hepatitis B, and hepatitis C and cytomegalovirus screening were all negative. Ova and parasite stool examination revealed Blastocystis sp. The patient was given intravenous metronidazole with clinical improvement by day three and total resolution of symptoms by day ten...
2016: Case Reports in Infectious Diseases
Hisao Yoshikawa, Yukiko Koyama, Erika Tsuchiya, Kazutoshi Takami
Blastocystis is a common unicellular eukaryotic parasite found not only in humans, but also in various kinds of animal species worldwide. Since Blastocystis isolates are morphologically indistinguishable, many molecular biological approaches have been applied to classify these isolates. The complete or partial sequences of the small subunit rRNA gene (SSU rDNA) are mainly used for comparisons and phylogenetic analyses among Blastocystis isolates. However, various lengths of the partial SSU rDNA sequence have been used for phylogenetic inference among genetically different isolates...
April 15, 2016: Parasitology International
Hisao Yoshikawa, Masaharu Tokoro, Takehiro Nagamoto, Shunsuke Arayama, Puji B S Asih, Ismail E Rozi, Din Syafruddin
Blastocystis sp. is a common parasite found in human and animal fecal samples. Currently, human Blastocystis isolates are classified into nine subtypes (STs) based on the phylogeny of their small subunit ribosomal RNA genes (SSU rDNAs). Since eight of the nine STs, except for ST9, have been reported in both humans and animals, these parasites are considered to be potentially zoonotic STs. To evaluate whether zoonotic transmissions play a main role in the lifecycle of Blastocystis, STs derived from humans, domestic pigs, domestic chickens, and wild rodents in a community with poor hygiene in Sumba Island, Indonesia were surveyed...
April 11, 2016: Parasitology International
Mariana F Pavanelli, Edilson Nobuyoshi Kaneshima, Carla F Uda, Cristiane M Colli, Ana L Falavigna-Guilherm, Mônica L Gomes
The pathogenic potential of Blastocystis sp. in experimental models requires further investigation. In this work, the pathogenicity of this parasite in the gastrointestinal tract of male Swiss mice was evaluated according to the inoculum size and period of infection. Animals were infected intragastrically, with 100, 500, 1,000, 5,000 and 10,000 Blastocystis sp. vacuolar forms obtained from a mixture of eight human isolates cultured axenically in Jones' medium. After seven, 14, 21, 28 and 60 days of infection, the animals were sacrificed and fragments of the small intestine (duodenum), large intestine, and cecum were subjected to histopathological analysis...
December 2015: Revista do Instituto de Medicina Tropical de São Paulo
Kalimuthu Nithyamathi, Samudi Chandramathi, Suresh Kumar
BACKGROUND: One of the largest cross-sectional study in recent years was carried out to investigate the prevalence of intestinal parasitic infections among urban and rural school children from five states namely Selangor, Perak, Pahang, Kedah and Johor in Peninsula Malaysia. This information would be vital for school authorities to influence strategies for providing better health especially in terms of reducing intestinal parasitism. METHODS AND PRINCIPAL FINDINGS: A total of 3776 stool cups was distributed to 26 schools throughout the country...
2016: PloS One
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