Glutamate transpoter

Progenitors within the neocortical ventricular zone (VZ) first generate pyramidal neurons and then astrocytes. We applied novel piggyBac transposase lineage tracking methods to fate-map progenitor populations positive for Nestin or glutamate and aspartate transpoter (GLAST) promoter activities in the rat neocortex. GLAST+ and Nestin+ progenitors at embryonic day 13 (E13) produce lineages containing similar rations of neurons and astrocytes. By E15, the GLAST+ progenitor population diverges significantly to produce lineages with 5-10-fold more astrocytes relative to neurons than generated by the Nestin+ population...

The present study examined the relationship between vesicular glutamate transpoter-3 (VGLUT3) positive cells and the activation of neurons in the brainstem and amygdala by bitter taste, using double-labeling immunohistochemistry. Conscious animals were subjected to intraoral bitter taste stimulation with quinine solution. Following this, neuronal activation was assessed by c-Fos expression and an analysis of c-Fos expression cells, VGLUT3 positive cells and double-labeled cells was made in the nucleus of the solitary tract (NST), the parabrachial nucleus (PBN) and amygdala...

OBJECTIVE: To examine the role of glutaminergic neurons in the transmission and integration of the sweat taste information in the brain stem and the amygdala. METHODS: Conscious Sprague-Dawley rats were subjected to oral sweet taste or water (control) stimulations. The activated neurons were identified by detecting c-Fos expression in taste-related brain areas, and the glutaminergic neurons by detecting vesicular glutamate transpoter-3 (VGLUT3). RESULTS: Compared with control group, the rats with oral sucrose solution stimulation exhibited significantly increased c-Fos-expressing and double-labeled neurons in the nucleus of the solitary tract (NST), the parabrachial nucleus (PBN) and the amygdala...