keyword
MENU ▼
Read by QxMD icon Read
search

Cilia and autophagy

keyword
https://www.readbyqxmd.com/read/28913352/new-roles-of-the-primary-cilium-in-autophagy
#1
REVIEW
Yenniffer Ávalos, Daniel Peña-Oyarzun, Mauricio Budini, Eugenia Morselli, Alfredo Criollo
The primary cilium is a nonmotile organelle that emanates from the surface of multiple cell types and receives signals from the environment to regulate intracellular signaling pathways. The presence of cilia, as well as their length, is important for proper cell function; shortened, elongated, or absent cilia are associated with pathological conditions. Interestingly, it has recently been shown that the molecular machinery involved in autophagy, the process of recycling of intracellular material to maintain cellular and tissue homeostasis, participates in ciliogenesis...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28855375/shear-stress-induces-non-canonical-autophagic-flux-in-intestinal-epithelial-monolayers
#2
Sun Wook Kim, Jonathan Ehrman, Mok-Ryeon Ahn, Jumpei Kondo, Andrea A Mancheno Lopez, Yun Sik Oh, Hanjune X Kim, Scott W Crawley, James R Goldenring, Matthew J Tyska, Erin C Rericha, Ken S Lau
Flow of fluids through the gut, such as milk from a neonatal diet, generates a shear stress on the unilaminar epithelium lining the lumen.  We report that exposure to physiological levels of fluid shear stress leads to the formation of large vacuoles, containing extracellular content, within polarizing intestinal epithelial cell monolayers.  These observations lead to two questions: how can cells lacking primary cilia transduce shear stress, and what molecular pathways support the formation of vacuoles that can exceed 80% of the cell volume...
August 30, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28811097/type-i-collagen-promotes-primary-cilia-growth-through-down-regulating-hdac6-mediated-autophagy-in-confluent-mouse-embryo-fibroblast-3t3-l1-cells
#3
Qian Xu, Weiwei Liu, Xiaoling Liu, Wuxiyar Otkur, Toshihiko Hayashi, Masayuki Yamato, Hitomi Fujisaki, Shunji Hattori, Shin-Ichi Tashiro, Takashi Ikejima
Primary cilia are microtubule-based organelles that extend from nearly all vertebrate cells. Abnormal ciliogenesis and cilia length are suggested to be associated with hypertension and obesity as well as diseases such as Meckel-Gruber syndrome. Extracellular matrix (ECM), comprising cellular microenvironment, influences cell shape and proliferation. However, influence of ECM on cilia biogenesis has not been well studied. In this study we examined the effects of type I collagen (col I), the major component of ECM, on primary cilia growth...
August 12, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28566325/persistent-mtorc1-signaling-in-cell-senescence-results-from-defects-in-amino-acid-and-growth-factor-sensing
#4
Bernadette Carroll, Glyn Nelson, Yoana Rabanal-Ruiz, Olena Kucheryavenko, Natasha A Dunhill-Turner, Charlotte C Chesterman, Qabil Zahari, Tong Zhang, Sarah E Conduit, Christina A Mitchell, Oliver D K Maddocks, Penny Lovat, Thomas von Zglinicki, Viktor I Korolchuk
Mammalian target of rapamycin complex 1 (mTORC1) and cell senescence are intimately linked to each other and to organismal aging. Inhibition of mTORC1 is the best-known intervention to extend lifespan, and recent evidence suggests that clearance of senescent cells can also improve health and lifespan. Enhanced mTORC1 activity drives characteristic phenotypes of senescence, although the underlying mechanisms responsible for increased activity are not well understood. We have identified that in human fibroblasts rendered senescent by stress, replicative exhaustion, or oncogene activation, mTORC1 is constitutively active and resistant to serum and amino acid starvation...
July 3, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28560694/role-of-apoptosis-in-the-development-of-autosomal-dominant-polycystic-kidney-disease-adpkd
#5
REVIEW
Lukas Peintner, Christoph Borner
Autosomal dominant polycystic kidney disease (ADPKD) is a widespread genetic disorder in the Western world and is characterized by cystogenesis that often leads to end-stage renal disease (ESRD). Mutations in the pkd1 gene, encoding for polycystin-1 (PC1) and its interaction partner pkd2, encoding for polycystin-2 (PC2), are the main drivers of this disease. PC1 and PC2 form a multiprotein membrane complex at cilia sites of the plasma membrane and at intracellular membranes. This complex mediates calcium influx and stimulates various signaling pathways regulating cell survival, proliferation and differentiation...
July 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28361305/role-of-primary-cilia-in-non-dividing-and-post-mitotic-cells
#6
REVIEW
Gerd Walz
The essential role of primary (non-motile) cilia during the development of multi-cellular tissues and organs is well established and is underlined by severe disease manifestations caused by mutations in cilia-associated molecules that are collectively termed ciliopathies. However, the role of primary cilia in non-dividing and terminally differentiated, post-mitotic cells is less well understood. Although the prevention of cells from re-entering the cell cycle may represent a major chore, primary cilia have recently been linked to DNA damage responses, autophagy and mitochondria...
July 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28134067/ultrastructural-features-of-the-tomont-of-cryptocaryon-irritans-ciliophora-prostomatea-a-parasitic-ciliate-of-marine-fishes
#7
Rui Ma, Xinpeng Fan, Fei Yin, Bing Ni, Fukang Gu
Numerous studies have been conducted on the cellular morphology of Cryptocaryon irritans. However, details regarding the tomont stage of its life cycle remain lacking. In this study, we investigated the morphology of the tomont stage throughout encystment and cell division using light and electron microscopy. Results showed that there was no secretion of encystation-specific secretory vesicles or extrusomes during formation of the cyst wall. Instead, the synthesis and construction of the C. irritans cyst wall materials may involve molecular events at the pellicle...
May 2017: Parasitology
https://www.readbyqxmd.com/read/27816963/defective-ciliogenesis-in-thyroid-h%C3%A3-rthle-cell-tumors-is-associated-with-increased-autophagy
#8
Junguee Lee, Shinae Yi, Yea Eun Kang, Joon Young Chang, Jung Tae Kim, Hae Joung Sul, Jong Ok Kim, Jin Man Kim, Joon Kim, Anna Maria Porcelli, Koon Soon Kim, Minho Shong
Primary cilia are found in the apical membrane of thyrocytes, where they may play a role in the maintenance of follicular homeostasis. In this study, we examined the distribution of primary cilia in the human thyroid cancer to address the involvement of abnormal ciliogenesis in different thyroid cancers. We examined 92 human thyroid tissues, including nodular hyperplasia, Hashimoto's thyroiditis, follicular tumor, Hürthle cell tumor, and papillary carcinoma to observe the distribution of primary cilia. The distribution and length of primary cilia facing the follicular lumen were uniform across variable-sized follicles in the normal thyroid gland...
November 29, 2016: Oncotarget
https://www.readbyqxmd.com/read/27682589/intraflagellar-transport-protein-ift20-is-essential-for-male-fertility-and-spermiogenesis-in-mice
#9
Zhengang Zhang, Wei Li, Yong Zhang, Ling Zhang, Maria E Teves, Hong Liu, Jerome F Strauss, Gregory J Pazour, James A Foster, Rex A Hess, Zhibing Zhang
Intraflagellar transport (IFT) is a conserved mechanism thought to be essential for the assembly and maintenance of cilia and flagella. However, little is known about its role in mammalian sperm flagella formation. To fill this gap, we disrupted the Ift20 gene in male germ cells. Homozygous mutant mice were infertile with significantly reduced sperm counts and motility. In addition, abnormally shaped elongating spermatid heads and bulbous round spermatids were found in the lumen of the seminiferous tubules...
September 28, 2016: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/27514915/methods-to-study-interactions-between-ciliogenesis-and-autophagy
#10
Birgit Hegner Satir, Olatz Pampliega
Autophagy is a catabolic pathway for the degradation and recycling of intracellular components, contributing to maintain cell homeostasis. Changes in autophagy activity can be monitored by a variety of biochemical and functional assays that should be used in combination. Recently, it has been described that signaling from the primary cilium modulates autophagy. This novel and reciprocal interaction will impact diverse aspects of the cell biology in healthy and pathophysiological conditions. Here, we describe methods to monitor autophagy activity in cilia mutants, as well as the use of autophagy mutants to monitor ciliogenesis...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27435857/silibinin-negatively-contributes-to-primary-cilia-length-via-autophagy-regulated-by-histone-deacetylase-6-in-confluent-mouse-embryo-fibroblast-3t3-l1-cells
#11
Qian Xu, Wei Liu, Xiaoling Liu, Weiwei Liu, Hongju Wang, Guodong Yao, Linghe Zang, Toshihiko Hayashi, Shin-Ichi Tashiro, Satoshi Onodera, Takashi Ikejima
Primary cilium is a cellular antenna, signalling as a sensory organelle. Numerous pathological manifestation is associated with change of its length. Although the interaction between autophagy and primary cilia has been suggested, the role of autophagy in primary cilia length is largely unknown. In this study the primary cilia were immunostained and observed by using confocal fluorescence microscopy, and we found that silibinin, a natural flavonoid, shortened the length of primary cilia, meanwhile it also induced autophagy in 3T3-L1 cells...
September 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/27230529/primary-cilia-mechanosensing-triggers-autophagy-regulated-cell-volume-control
#12
Zsuzsanna Takacs, Tassula Proikas-Cezanne
The primary cilium and the process of autophagy are thought to be in a functionally reciprocal relationship. In further support of this link, fluid flow sensing by the primary cilium is now shown to induce autophagy, which in turn regulates the volume of kidney epithelial cells.
May 27, 2016: Nature Cell Biology
https://www.readbyqxmd.com/read/26632497/aberrant-protein-trafficking-in-retinal-degenerations-the-initial-phase-of-retinal-remodeling
#13
REVIEW
Katie L Bales, Alecia K Gross
Retinal trafficking proteins are involved in molecular assemblies that govern protein transport, orchestrate cellular events involved in cilia formation, regulate signal transduction, autophagy and endocytic trafficking, all of which if not properly controlled initiate retinal degeneration. Improper function and or trafficking of these proteins and molecular networks they are involved in cause a detrimental cascade of neural retinal remodeling due to cell death, resulting as devastating blinding diseases. A universal finding in retinal degenerative diseases is the profound detection of retinal remodeling, occurring as a phased modification of neural retinal function and structure, which begins at the molecular level...
September 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/26160070/primary-cilia-and-autophagic-dysfunction-in-huntington-s-disease
#14
REVIEW
M Kaliszewski, A B Knott, E Bossy-Wetzel
Huntington's disease (HD) is an inherited, neurodegenerative disorder caused by a single-gene mutation: a CAG expansion in the huntingtin (HTT) gene that results in production of a mutated protein, mutant HTT, with a polyglutamine tail (polyQ-HTT). Although the molecular pathways of polyQ-HTT toxicity are not fully understood, because protein misfolding and aggregation are central features of HD, it has long been suspected that cellular housekeeping processes such as autophagy might be important to disease pathology...
September 2015: Cell Death and Differentiation
https://www.readbyqxmd.com/read/26157548/autophagy-regulates-formation-of-primary-cilia-in-mefloquine-treated-cells
#15
Ji Hyun Shin, Dong-Jun Bae, Eun Sung Kim, Han Byeol Kim, So Jung Park, Yoon Kyung Jo, Doo Sin Jo, Dong-Gyu Jo, Sang-Yeob Kim, Dong-Hyung Cho
Primary cilia have critical roles in coordinating multiple cellular signaling pathways. Dysregulation of primary cilia is implicated in various ciliopathies. To identify specific regulators of autophagy, we screened chemical libraries and identified mefloquine, an anti-malaria medicine, as a potent regulator of primary cilia in human retinal pigmented epithelial (RPE) cells. Not only ciliated cells but also primary cilium length was increased in mefloquine-treated RPE cells. Treatment with mefloquine strongly induced the elongation of primary cilia by blocking disassembly of primary cilium...
July 2015: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/26051179/skp2-mediated-raga-ubiquitination-elicits-a-negative-feedback-to-prevent-amino-acid-dependent-mtorc1-hyperactivation-by-recruiting-gator1
#16
Guoxiang Jin, Szu-Wei Lee, Xian Zhang, Zhen Cai, Yuan Gao, Ping-Chieh Chou, Abdol Hossein Rezaeian, Fei Han, Chi-Yun Wang, Juo-Chin Yao, Zhaohui Gong, Chia-Hsin Chan, Chih-Yang Huang, Fuu-Jen Tsai, Chang-Hai Tsai, Shih-Hsin Tu, Chih-Hsiung Wu, Dos D Sarbassov, Yuan-Soon Ho, Hui-Kuan Lin
The regulation of RagA(GTP) is important for amino-acid-induced mTORC1 activation. Although GATOR1 complex has been identified as a negative regulator for mTORC1 by hydrolyzing RagA(GTP), how GATOR1 is recruited to RagA to attenuate mTORC1 signaling remains unclear. Moreover, how mTORC1 signaling is terminated upon amino acid stimulation is also unknown. We show that the recruitment of GATOR1 to RagA is induced by amino acids in an mTORC1-dependent manner. Skp2 E3 ligase drives K63-linked ubiquitination of RagA, which facilitates GATOR1 recruitment and RagA(GTP) hydrolysis, thereby providing a negative feedback loop to attenuate mTORC1 lysosomal recruitment and prevent mTORC1 hyperactivation...
June 18, 2015: Molecular Cell
https://www.readbyqxmd.com/read/25906314/reciprocal-regulation-of-cilia-and-autophagy-via-the-mtor-and-proteasome-pathways
#17
Shixuan Wang, Man J Livingston, Yunchao Su, Zheng Dong
Primary cilium is an organelle that plays significant roles in a number of cellular functions ranging from cell mechanosensation, proliferation, and differentiation to apoptosis. Autophagy is an evolutionarily conserved cellular function in biology and indispensable for cellular homeostasis. Both cilia and autophagy have been linked to different types of genetic and acquired human diseases. Their interaction has been suggested very recently, but the underlying mechanisms are still not fully understood. We examined autophagy in cells with suppressed cilia and measured cilium length in autophagy-activated or -suppressed cells...
April 3, 2015: Autophagy
https://www.readbyqxmd.com/read/25796328/bix-01294-induced-autophagy-regulates-elongation-of-primary-cilia
#18
Ji Hyun Shin, Pan Soo Kim, Eun Sung Kim, So Jung Park, Yoon Kyung Jo, Jung Jin Hwang, Tae Joo Park, Jong Wook Chang, Jin-Ho Seo, Dong-Hyung Cho
Previously, we showed that BIX-01294 treatment strongly activates autophagy. Although, the interplay between autophagy and ciliogenesis has been suggested, the role of autophagy in ciliogenesis is controversial and largely unknown. In this study, we investigated the effects of autophagy induced by BIX-01294 on the formation of primary cilia in human retinal pigmented epithelial (RPE) cells. Treatment of RPE cells with BIX-01294 caused strong elongation of the primary cilium and increased the number of ciliated cells, as well as autophagy activation...
May 1, 2015: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/25671433/inhibition-of-autophagy-suppresses-sertraline-mediated-primary-ciliogenesis-in-retinal-pigment-epithelium-cells
#19
Eun Sung Kim, Ji Hyun Shin, So Jung Park, Yoon Kyung Jo, Jae-Sung Kim, Il-Hwan Kang, Jung-Bum Nam, Doo-Young Chung, Yoonchul Cho, EunJoo H Lee, Jong Wook Chang, Dong-Hyung Cho
Primary cilia are conserved cellular organelles that regulate diverse signaling pathways. Autophagy is a complex process of cellular degradation and recycling of cytoplasmic proteins and organelles, and plays an important role in cellular homeostasis. Despite its potential importance, the role of autophagy in ciliogenesis is largely unknown. In this study, we identified sertraline as a regulator of autophagy and ciliogenesis. Sertraline, a known antidepressant, induced the growth of cilia and blocked the disassembly of cilia in htRPE cells...
2015: PloS One
https://www.readbyqxmd.com/read/25617802/autophagy-in-lung-disease-pathogenesis-and-therapeutics
#20
Stefan W Ryter, Augustine M K Choi
Autophagy, a cellular pathway for the degradation of damaged organelles and proteins, has gained increasing importance in human pulmonary diseases, both as a modulator of pathogenesis and as a potential therapeutic target. In this pathway, cytosolic cargos are sequestered into autophagosomes, which are delivered to the lysosomes where they are enzymatically degraded and then recycled as metabolic precursors. Autophagy exerts an important effector function in the regulation of inflammation, and immune system functions...
2015: Redox Biology
keyword
keyword
57503
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"