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beta glucuronidase

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https://www.readbyqxmd.com/read/29666330/whitefly-and-aphid-inducible-promoters-of-arabidopsis-thaliana-l
#1
Neeraj Kumar Dubey, Devesh Kumar Mishra, Asif Idris, Deepti Nigam, Pradhyumna Kumar Singh, Samir V Sawant
Lack of regulated expression and tissue specificity are the major drawbacks of plant and virus-derived constitutive promoters. A precise tissue or site-specific expression, facilitate regulated expression of proteins at the targeted time and site. Publically available microarray data on whitefly and aphid infested Arabidopsis thaliana L. was used to identify whitefly and aphid-inducible genes. The qRT-PCR further validated the inducible behaviour of these genes under artificial infestation. Promoter sequences of genes were retrieved from the Arabidopsis Information Resources database with their corresponding 5'UTR and cloned from the A...
March 2018: Journal of Genetics
https://www.readbyqxmd.com/read/29565959/identifying-levorphanol-ingestion-using-urine-biomarkers-in-health-care-patients
#2
Amber R Watson, Ali Roberts
BACKGROUND: Levorphanol is a long-acting opioid analgesic that is an optical isomer of dextrorphan, a metabolite of the over-the-counter cough suppressant dextromethorphan. Providers prescribing levorphanol for pain management may need to assess compliance through urine drug testing, as this agent is subject to abuse. Therefore, it is important to differentiate between dextromethorphan and levorphanol ingestion. OBJECTIVES: This article is the first to report urine concentrations of levorphanol/dextrorphan and 3-hydroxymorphinan in human urine and assesses the need for an enantiomeric analysis to distinguish between dextromethorphan and levorphanol ingestion...
March 2018: Pain Physician
https://www.readbyqxmd.com/read/29437777/trehalose-6-phosphate-regulates-photosynthesis-and-assimilate-partitioning-in-reproductive-tissue
#3
Maria Oszvald, Lucia F Primavesi, Cara A Griffiths, Jonathan Cohn, Shib Basu, Michael L Nuccio, Matthew J Paul
Transgenic maize expressing a rice trehalose phosphate phosphatase (TPP1) controlled by a rice MADS6 promoter active over the 2- to 3-week flowering period are higher yielding than wild type with or without drought during flowering. To better understand the mechanistic basis of the yield increase, we characterise gene expression and metabolite profiles in developing female reproductive tissue comprising florets, node, pith and shank and in leaves over the flowering period with and without drought. Using the β-glucuronidase reporter the MADS6 promoter activity was most closely linked to vasculature, particularly phloem companion cells in florets and pith consistent with largest decreases in trehalose 6-phosphate (T6P) levels (2-3 fold) in pith and florets...
February 6, 2018: Plant Physiology
https://www.readbyqxmd.com/read/29422909/depletion-of-arabidopsis-acyl-coa-binding-protein3-affects-fatty-acid-composition-in-the-phloem
#4
Tai-Hua Hu, Shiu-Cheung Lung, Zi-Wei Ye, Mee-Len Chye
Oxylipins are crucial components in plant wound responses that are mobilised via the plant vasculature. Previous studies have shown that the overexpression of an Arabidopsis acyl-CoA-binding protein, AtACBP3, led to an accumulation of oxylipin-containing galactolipids, and AtACBP3pro::BETA-GLUCURONIDASE (GUS) was expressed in the phloem of transgenic Arabidopsis. To investigate the role of AtACBP3 in the phloem, reverse transcription-polymerase chain reaction and western blot analysis of phloem exudates from the acbp3 mutant and wild type revealed that the AtACBP3 protein, but not its mRNA, was detected in the phloem sap...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29372514/gene-cloning-system-for-sulfonamide-mineralizing-microbacterium-sp-strain-br1
#5
I Ostash, B Kolvenbach, P F-X Corvini, V Fedorenko, B Ostash, Danuta Cichocka
The wide application of sulfonamide (SA) antibiotics in human and veterinary medicine contributes to the accumulation of these antibiotics in the environment and the corresponding onset of antibiotic resistance among bacteria. Microbacterium sp. BR1 is capable of mineralizing sulfamethoxazole and other SAs via a novel mechanism. The genetic basis of SA elimination by BR1 remains unknown. Development of an efficient plasmid transfer protocol for Microbacterium sp. BR1 is highly desirable, as it would open the door to genetic analysis and manipulation of its genome...
January 25, 2018: Journal of Applied Genetics
https://www.readbyqxmd.com/read/29161267/sugar-industry-sponsorship-of-germ-free-rodent-studies-linking-sucrose-to-hyperlipidemia-and-cancer-an-historical-analysis-of-internal-documents
#6
Cristin E Kearns, Dorie Apollonio, Stanton A Glantz
In 1965, the Sugar Research Foundation (SRF) secretly funded a review in the New England Journal of Medicine that discounted evidence linking sucrose consumption to blood lipid levels and hence coronary heart disease (CHD). SRF subsequently funded animal research to evaluate sucrose's CHD risks. The objective of this study was to examine the planning, funding, and internal evaluation of an SRF-funded research project titled "Project 259: Dietary Carbohydrate and Blood Lipids in Germ-Free Rats," led by Dr...
November 2017: PLoS Biology
https://www.readbyqxmd.com/read/29158804/heparanase-contributes-to-trans-endothelial-migration-of-hepatocellular-carcinoma-cells
#7
Xiaopeng Chen, Wen Jiang, Chaofu Yue, Wenjun Zhang, Chaogang Tong, Dafei Dai, Bin Cheng, Chen Huang, Linming Lu
The overall outcome of patients with hepatocellular carcinoma (HCC) is still very poor due to its high metastasis and recurrence rate. During metastasis, trans-endothelial migration (TEM) of HCC cells is a key step. Heparanase (HPSE) is an endo-beta-glucuronidase and exerts prometastatic properties for normal and tumor-derived cells. However, it is remains unclear that HPSE contributes to TEM of HCC cells. In this study, human umbilical vein endothelial cells-C (HUVEC-C) was used to simulate vascular endothelial cells (VECs), and the HCCLM3 cells with high HPSE expression were chosen and used for in vitro TEM assay and in vivo experiment...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/29125673/overexpression-of-sdh-confers-tolerance-to-salt-and-osmotic-stress-but-decreases-aba-sensitivity-in-arabidopsis
#8
Xiao-Pu Shi, Jing-Jing Ren, Qin Yu, Shu-Mei Zhou, Qiu-Ping Ren, Lan-Jing Kong, Xiu-Ling Wang
Sorbitol dehydrogenase (SDH) catalyses the reversible oxidation of sorbitol, xylitol and ribitol to their corresponding ketoses. In this study, we investigated the expression and role of Arabidopsis SDH in salt and osmotic stress tolerance, and abscisic acid (ABA) response. The expression patterns of SDH were investigated using transgenic Arabidopsis plants expressing beta-glucuronidase (GUS) under control of the promoter with the first intron of SDH. qRT-PCR and histochemical assay of GUS activity were used to study SDH expression regulation by ABA, salt and osmotic stress...
November 10, 2017: Plant Biology
https://www.readbyqxmd.com/read/29090013/a-sensitive-synthetic-reporter-for-visualizing-cytokinin-signaling-output-in-rice
#9
Jinyuan Tao, Huwei Sun, Pengyuan Gu, Zhihao Liang, Xinni Chen, Jiajing Lou, Guohua Xu, Yali Zhang
BACKGROUND: Cytokinins play many essential roles in plant growth and development, mainly through signal transduction pathways. Although the cytokinin signaling pathway in rice has been clarified, no synthetic reporter for cytokinin signaling output has been reported for rice. The sensitive synthetic reporter two-component signaling sensor ( TCSn ) is used in the model plant Arabidopsis; however, whether the reporter reflects the cytokinin signaling output pattern in rice remains unclear...
2017: Plant Methods
https://www.readbyqxmd.com/read/29079617/-staphylococcus-edaphicus-sp-nov-isolated-in-antarctica-harbours-mecc-gene-and-genomic-islands-with-suspected-role-in-adaptation-to-extreme-environment
#10
Roman Pantůček, Ivo Sedláček, Adéla Indráková, Veronika Vrbovská, Ivana Mašlaňová, Vojtěch Kovařovic, Pavel Švec, Stanislava Králová, Lucie Krištofová, Jana Kekláková, Petr Petráš, Jiří Doškař
Two Gram-stain-positive, coagulase-negative staphylococcal strains were isolated from abiotic sources, stone fragments and sandy soil in James Ross Island, Antarctica. Here we describe properties of novel species of the genus Staphylococcus that has near identical 16S rRNA gene sequence to Staphylococcus saprophyticus However, compared to S. saprophyticus and the next closest relatives, the new species demonstrates considerable phylogenetic distance at whole genome level, average nucleotide identity <85 %, and inferred DNA-DNA hybridization <30%...
October 27, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29078076/involvement-of-bilitranslocase-and-beta-glucuronidase-in-the-vascular-protection-by-hydroxytyrosol-and-its-glucuronide-metabolites-in-oxidative-stress-conditions
#11
Julien Peyrol, Grégory Meyer, Philippe Obert, Olivier Dangles, Laurent Pechère, Marie-Josèphe Amiot, Catherine Riva
Olive oil vascular benefits have been attributed to hydroxytyrosol (HT). However, HT biological actions are still debated because it is extensively metabolized into glucuronides (GCs). The aim of this study was to test HT and GC vasculoprotective effects and the underlying mechanisms using aorta rings from 8-week-old male Wistar rats. In the absence of oxidative stress, incubation with 100 μM HT or GC for 5 min did not exert any vasorelaxing effect and did not influence the vascular function. Conversely, in condition of oxidative stress [upon incubation with 500 μM tert-butylhydroperoxide (t-BHP) for 30 min], preincubation with HT or GC improved acetylcholine-induced vasorelaxation compared with untreated samples (no t-BHP)...
January 2018: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29053757/generation-of-composite-persea-americana-mill-avocado-plants-a-proof-of-concept-study
#12
S Ashok Prabhu, Buyani Ndlovu, Juanita Engelbrecht, Noëlani van den Berg
Avocado (Persea americana (Mill.)), an important commercial fruit, is severely affected by Phytophthora Root Rot in areas where the pathogen is prevalent. However, advances in molecular research are hindered by the lack of a high-throughput transient transformation system in this non-model plant. In this study, a proof-of-concept is demonstrated by the successful application of Agrobacterium rhizogenes-mediated plant transformation to produce composite avocado plants. Two ex vitro strategies were assessed on two avocado genotypes (Itzamna and A0...
2017: PloS One
https://www.readbyqxmd.com/read/28962937/beta-glucuronidase-activity-in-dried-blood-spots-reduced-technique-with-biochemical-parameters-determined
#13
Jaqueline Cé, Melissa Tôrres Rodrigues, Eduarda Tassoni Käfer, Vitória da Costa Moraes, Janice Carneiro Coelho
INTRODUCTION: Mucopolysaccharidoses (MPS) occur due to deficiency in the activity of enzymes that catalyze the breakdown of glycosaminoglycans. MPS VII is caused by deficiency of the beta-glucuronidase enzyme (GUSB). OBJECTIVES: This study aimed to enhance the technique to measure GUSB activity by reducing the amount of reagents and the size of the DBS, as well as to determine some biochemical parameters of enzyme of healthy individuals. METHODS: The measurement of GUSB in 3 and 1...
December 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28770119/first-report-on-fetal-cerebral-polyglucosan-bodies-in-mucopolysaccharidosis-type-vii
#14
Hazim Kadhim, Valérie Segers, Catheline Vilain, Julie Désir, Nicky D'Haene
We report on the detection of discordant inclusions in the brain of a 25-week female fetus with a very rare lysosomal storage disease, namely, Sly disease (mucopolysaccharidosis (MPS) type VII), presenting with nonimmune hydrops fetalis. Besides vacuolated neurons, we found abundant deposition of polyglucosan bodies (PGBs) in the developing brain of this fetus in whom MPS-VII was corroborated by lysosomal beta-glucuronidase-deficiency detected in fetal blood and fetal skin-fibroblasts and by the presence of a heterozygous pathogenic variant in the GUSB gene in the mother...
2017: Case Reports in Pediatrics
https://www.readbyqxmd.com/read/28755354/transient-gus-gene-expression-in-date-palm-fruit-using-agroinjection-transformation-technique
#15
Mohei El-Din M Solliman, Hebaallah A Mohasseb, Abdullatif A Al-Khateeb, Jameel M Al-Khayri, Suliman A Al-Khateeb
Transient expression of foreign genes in plant tissue is a valuable tool for testing the efficacy of transformation methods. In this work, we present, for the first time, the utilization of agroinjection as an efficient transformation system for gene delivery in date palm fruit. The research utilized Agrobacterium tumefaciens strain LBA4404 harboring the binary vector pRI201-AN-GUS carrying the beta-glucuronidase (GUS) gene, under the control of a CaMV 35S and kanamycin (NPTII) as an antibiotic gene under the control of a NOS promoter...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28482050/parallel-assessment-of-the-effects-of-bisphenol-a-and-several-of-its-analogs-on-the-adult-human-testis
#16
C Desdoits-Lethimonier, L Lesné, P Gaudriault, D Zalko, J P Antignac, Y Deceuninck, C Platel, N Dejucq-Rainsford, S Mazaud-Guittot, B Jégou
STUDY QUESTION: Are bisphenol A (BPA) and BPA analogs (BPA-A) safe for male human reproductive function? SUMMARY ANSWER: The endocrine function of human testes explants [assessed by measuring testosterone and insulin-like factor 3 (INSL3)] was impacted by exposure of the human adult testis explants to BPA/BPA-A. WHAT IS KNOWN ALREADY: The few epidemiologic studies performed suggest that bisphenols have potential endocrine disruptive properties, but they did not identify clear and direct patterns of endocrine disruption...
July 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28075052/functional-characterisation-and-cell-specificity-of-bvsut1-the-transporter-that-loads-sucrose-into-the-phloem-of-sugar-beet-beta-vulgaris-l-source-leaves
#17
P Nieberl, C Ehrl, B Pommerrenig, D Graus, I Marten, B Jung, F Ludewig, W Koch, K Harms, U-I Flügge, H E Neuhaus, R Hedrich, N Sauer
Sugar beet (Beta vulgaris L.) is one of the most important sugar-producing plants worldwide and provides about one third of the sugar consumed by humans. Here we report on molecular characterisation of the BvSUT1 gene and on the functional characterisation of the encoded transporter. In contrast to the recently identified tonoplast-localised sucrose transporter BvTST2.1 from sugar beet taproots, which evolved within the monosaccharide transporter (MST) superfamily, BvSUT1 represents a classical sucrose transporter and is a typical member of the disaccharide transporter (DST) superfamily...
January 11, 2017: Plant Biology
https://www.readbyqxmd.com/read/28006931/pharmacokinetics-of-boldine-in-control-and-mrp2-deficient-rats
#18
J Cermanova, A Prasnicka, E Dolezelova, L Rozkydalova, M Hroch, J Chládek, P Tomsik, I Kloeting, S Micuda
The aim of the present study was to describe the currently poorly understood pharmacokinetics (PK) of boldine in control rats (LW, Lewis rats), and Mrp2 transporter-deficient rats (TR(-)). Animals from the LW and TR(-) groups underwent a bolus dose study with 10 mg/kg of boldine applied either orally or intravenously in order to evaluate the major PK parameters. The TR(-) rats demonstrated significantly reduced total clearance with prolonged biological half-life (LW 12+/-4.6 versus TR(-) 20+/-4.4 min), decreased volume of distribution (LW 3...
December 21, 2016: Physiological Research
https://www.readbyqxmd.com/read/27978823/salivary-and-serum-inflammatory-mediators-among-pre-conception-women-with-periodontal-disease
#19
Hong Jiang, Yiming Zhang, Xu Xiong, Emily W Harville, Karmin O, Xu Qian
BACKGROUND: There have been inconsistent conclusions regarding the levels of inflammatory mediators in saliva and serum among people with or without periodontal disease. Although pre-conception has been put forward as the optimal time for the periodontal treatment in order to improving pregnancy outcomes, few studies have been conducted to examine inflammatory mediators in saliva and serum among pre-conception women. METHODS: Pre-conception women were recruited between January 2012 and December 2014...
December 15, 2016: BMC Oral Health
https://www.readbyqxmd.com/read/27648682/ocular-and-electrophysiological-findings-in-a-patient-with-sly-syndrome
#20
Maree Flaherty, Katie Geering, Stephanie Crofts, John Grigg
BACKGROUND: Sly syndrome (Mucopolysaccharidosis Type VII) is an autosomal recessive metabolic storage disorder due to mutations in the GUSB gene encoding the enzyme beta-glucuronidase. Deficiency of this lysosomal enzyme impairs the body's ability to break down the glycosaminoglycans - dermatan, heparan and chondroitin sulphate. Coarse facial features and macrocephaly are typically seen along with bony and skeletal abnormalities, including joint contractures and short stature. Widespread involvement occurs in many other tissues including cardiopulmonary, gastrointestinal, and neurological systems...
July 2017: Ophthalmic Genetics
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