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https://www.readbyqxmd.com/read/28922845/pot1-mediated-%C3%AE-integration-strategy-for-high-copy-stable-expression-of-heterologous-proteins-in-saccharomyces-cerevisiae
#1
Xiaofei Song, Quanli Liu, Jiwei Mao, Yuzhen Wu, Yuanzi Li, Kai Gao, Xiuming Zhang, Yanling Bai, Haijin Xu, Mingqiang Qiao
In biotechnological industry, increased expression cassette stability and copy number serve as important means of maintaining consistently high production levels of heterologous proteins in Saccharomyces cerevisiae. In this study, we combined δ sequences for site-specific integration with TPI1 gene from Schizosaccharomyces pombe (POT1) as a selection marker to realize high-copy integration and stable expression of heterologous proteins in S. cerevisiae. With the newly developed POT1 platform, a 32-copy integration of enhanced green fluorescent protein (EGFP) expression cassette was obtained in a single round and was stably maintained after 100 generations of growth in a rich complex medium...
September 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28922769/psb33-sustains-photosystem-ii-d1-protein-under-fluctuating-light-conditions
#2
Rikard Fristedt, Andrea Trotta, Marjaana Suorsa, Anders K Nilsson, Roberta Croce, Eva-Mari Aro, Björn Lundin
On Earth, solar irradiance varies as the sun rises and sets over the horizon, and sunlight is thus in constant fluctuation, following a slow dark-low-high-low-dark curve. Optimal plant growth and development are dependent on the capacity of plants to acclimate and regulate photosynthesis in response to these changes of light. Little is known of regulative processes for photosynthesis during nocturnal events. The nucleus-encoded plant lineage-specific protein PSB33 has been described as stabilizing the photosystem II complex, especially under light stress conditions, and plants lacking PSB33 have a dysfunctional state transition...
July 10, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28922739/o-glcnac-transferase-regulates-transcriptional-activity-of-human-oct4
#3
Sandii Constable, Jae-Min Lim, Krithika Vaidyanathan, Lance Wells
O-linked β-N-acetylglucosamine (O-GlcNAc) is a single sugar modification found on many different classes of nuclear and cytoplasmic proteins. Addition of this modification, by the enzyme O-linked N-acetylglucosamine transferase (OGT), is dynamic and inducible. One major class of proteins modified by O-GlcNAc is transcription factors. O-GlcNAc regulates transcription factor properties through a variety of different mechanisms including localization, stability and transcriptional activation. Maintenance of embryonic stem (ES) cell pluripotency requires tight regulation of several key transcription factors, many of which are modified by O-GlcNAc...
October 1, 2017: Glycobiology
https://www.readbyqxmd.com/read/28922729/lithium-induced-oxidative-damage-and-inflammation-in-the-rat-s-heart-protective-effect-of-grape-seed-and-skin-extract
#4
Ali Mezni, Hanène Aoua, Olfa Khazri, Ferid Limam, Ezzeddine Aouani
Lithium (Li) is a relevant mood stabilizer metal for the treatment of bipolar disorder (BD), as it protects from both depression and mania and reduces the risk of suicide. However, Lihas some clinical concerns as a narrow therapeutic index requiring routine monitoring of the serum level. The present study was designed to analyze the cardio-toxic side effect of Li and the ability of grape seed and skin extract (GSSE) to protect the heart against such toxicity. After 30days of exposure to Li (0, 2, 5 and 100mg/kg bw) and prevention with GSSE (4000mg/kg bw), rats were killed by decapitation and their heart processed for Li-induced oxidative stress...
September 14, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28922646/synthesis-spectral-characterization-and-computed-optical-analysis-of-potent-triazole-based-compounds
#5
Sajjad H Sumrra, Fazila Mushtaq, Muhammad Khalid, Muhammad Asam Raza, Muhammad Faizan Nazar, Bakhat Ali, Ataualpa A C Braga
Biologically active triazole Schiff base ligand (L) and metal complexes [Fe(II), Co(II), Ni(II), Cu(II) and Zn(II)] are reported herein. The ligand acted as tridentate and coordinated towards metallic ions via azomethine-N, triazolic-N moiety and deprotonated-O of phenyl substituents in an octahedral manner. These compounds were characterized by physical, spectral and analytical analysis. The synthesized ligand and metal complexes were screened for antibacterial pathogens against Chromohalobacter salexigens, Chromohalobacter israelensi, Halomonas halofila and Halomonas salina, antifungal bioassay against Aspergillus niger and Aspergellus flavin, antioxidant (DPPH, phosphomolybdate) and also for enzyme inhibition [butyrylcholinesterase (BChE) and acetylcholinesterase (AChE)] studies...
September 13, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28922641/structural-luminescence-thermodynamic-and-theoretical-studies-on-mononuclear-complexes-of-eu-iii-with-pyridine-monocarboxylate-n-oxides-in-aqueous-solution
#6
Rama Mohana Rao Dumpala, Neetika Rawat, Anil Boda, Sk Musharaf Ali, B S Tomar
The mononuclear complexes formed by Eu(III) with three isomeric pyridine monocarboxylate-N-oxides namely picolinic acid-N-oxide (PANO), nicotinic acid-N-oxide (NANO) and isonicotinic acid-N-oxide (IANO) in aqueous solutions were studied by potentiometry, luminescence spectroscopy and isothermal titration calorimetry (ITC) to determine the speciation, coordination, luminescence properties and thermodynamic parameters of the complexes formed during the course of the reaction. More stable six membered chelate complexes with stoichiometry (MLi, i=1-4) are formed by Eu(III) with PANO while non chelating ML and ML2 complexes are formed by NANO and IANO...
September 9, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28922638/reverse-flow-injection-spectrophotometric-determination-of-ciprofloxacin-in-pharmaceuticals-using-iron-from-soil-as-a-green-reagent
#7
Sysay Palamy, Wirat Ruengsitagoon
A novel reverse flow injection spectrophotometric method for the determination of ciprofloxacin was successfully combined with the on-line introduction of an iron solution extracted from soil as green reagent. The assay was optimized by a univariate method to select the optimum conditions for the highest absorbance and highest stability of the complex. Beer-Lambert's law (λmax=440nm) is obeyed in the range 0.5-50μgmL(-1) with a correlation coefficient (r(2)) of 0.9976 and 0.9996 using soil as green reagent from Khon Kaen, Thailand and Vientiane, Laos, respectively...
September 13, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28922620/%C3%AE-pad-fluorescence-scattering-immunoagglutination-assay-for-cancer-biomarkers-from-blood-and-serum
#8
Cayla Baynes, Jeong-Yeol Yoon
A microfluidic paper analytical device (μPAD) was created for the sensitive quantification of cancer antigens, carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA 19-9), from human whole blood and serum, toward diagnosis and prognosis of colorectal cancer. Anti-CEA and anti-CA 19-9 antibodies were covalently linked to submicron, fluorescent polystyrene particles, loaded, and then dried in the center of the μPAD channel. CEA- or CA 19-9-spiked blood or serum samples were loaded to the inlet of μPAD, and subsequent immunoagglutination changed the fluorescent scatter signals upon ultraviolet (UV) excitation...
September 1, 2017: SLAS Technology
https://www.readbyqxmd.com/read/28922615/the-impact-of-incorporating-antimicrobials-into-implant-surfaces
#9
N J Hickok, I M Shapiro, A F Chen
With the increase in numbers of joint replacements, spinal surgeries, and dental implantations, there is an urgent need to combat implant-associated infection. In addition to stringent sterile techniques, an efficacious way to prevent this destructive complication is to create new implants with antimicrobial properties. Specifically, these implants must be active in the dental implant environment where the implant is bathed in the glycoprotein-rich salivary fluids that enhance bacterial adhesion, and propagation, and biofilm formation...
September 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28922606/three-dimensional-nico2o4-mnmoo4-core-shell-nanoarrays-for-high-performance-asymmetric-supercapacitors
#10
Yuliang Yuan, Weicheng Wang, Jie Yang, Haichao Tang, Zhizhen Ye, Yujia Zeng, Jianguo Lu
Design of new materials with sophisticated nanostructure has been proven to be an efficient strategy to improve their properties in many applications. Herein, we demonstrate the successful combination of high electron conductive materials of NiCo2O4 with the high capacitance materials of MnMoO4 by forming a core-shell nanostructure. The NiCo2O4@MnMoO4 core-shell nanoarrays (CSNAs) electrode possesses high specific capacitance of 4.24 F cm-2 at a current density of 5 mA cm-2, obviously larger than the pristine NiCo2O4 electrode...
September 18, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28922599/effect-of-t%C3%A2-t-mismatch-on-dna-dynamics-probed-by-minor-groove-binders-comparison-of-dynamic-stokes-shifts-of-hoechst-and-dapi
#11
Him Shweta, Moirangthem Kiran Singh, Kavita Yadav, Sachin Dev Verma, Nibedita Pal, Sobhan Sen
Recognition of DNA base mismatches and their subsequent repair by enzymes is vital for genomic stability. However, it is difficult to comprehend such process in which enzymes sense and repair different types of mismatches with different ability. It has been suggested that the differential structural changes of mismatched-bases act as cues to the repair enzymes, although effect of such DNA structural changes on surrounding water and ion dynamics is inevitable due to strong electrostatic coupling among them. Thus, collective dynamics of DNA, water and ions near the mismatch site is believed to be important for mismatch recognition and repair mechanism...
September 18, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28922598/proteolytic-unlocking-of-ultra-stable-twin-acylhydrazone-linkers-for-lysosomal-acid-triggered-release-of-anticancer-drugs
#12
Yiwu Zheng, Jing Ren, Yaqi Wu, Xiaoting Meng, Yibing Zhao, Chuanliu Wu
Targeted prodrugs exploiting cleavable linkers capable of responding to endogenous stimuli have increasingly been explored for cancer therapy. Successful application of these prodrug designs relies on the manipulation of both stability and responsiveness of the cleavable linkers, which, however, are difficult to be finely regulated, particularly for acid-responsive acylhydrazone bonds. Here we developed a new class of peptide-bridged twin-acylhydrazone linkers (PTA linkers) displaying both an ultrahigh stability and a rapid responsiveness-highly stable in neutral and acidic conditions due to effect of cooperativity between the two acylhydrazone bonds, easily cleavable in acidic conditions after enzymatically-triggered unlocking of the two bonds...
September 18, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28922576/anion-exchange-membranes-for-alkaline-fuel-cell-applications-the-effects-of-cations
#13
Zhe Sun, Bencai Lin, Feng Yan
Alkaline anion exchange membrane fuel cells (AEMFCs) are attracting great attention, because of their potential use of non-precious electrocatalysts. Anion exchange membrane (AEM) is one of the key components of AEMFCs. An ideal AEM should possess high hydroxide conductivity and sufficient long-term durability at elevated temperatures in high pH solutions. This review provides recent progresses of alkaline stability behavior of cations (including quaternary ammonium, imidazolium, guanidinium, pyridinium, tertiary sulfonium, phosphonium, benzimidazolium and pyrrolidinium) and their analogous AEMs, which have been investigated by both experimental studies and theoretical calculations...
September 18, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28922572/s-doped-tise2-nanoplates-fe3-o4-nanoparticles-heterostructure
#14
Jun Yang, Yufei Zhang, Yizhou Zhang, Jinjun Shao, Hongbo Geng, Yu Zhang, Yun Zheng, Mani Ulaganathan, Zhengfei Dai, Bing Li, Yun Zong, Xiaochen Dong, Qingyu Yan, Wei Huang
2D Sulfur-doped TiSe2 /Fe3 O4 (named as S-TiSe2 /Fe3 O4 ) heterostructures are synthesized successfully based on a facile oil phase process. The Fe3 O4 nanoparticles, with an average size of 8 nm, grow uniformly on the surface of S-doped TiSe2 (named as S-TiSe2 ) nanoplates (300 nm in diameter and 15 nm in thickness). These heterostructures combine the advantages of both S-TiSe2 with good electrical conductivity and Fe3 O4 with high theoretical Li storage capacity. As demonstrated potential applications for energy storage, the S-TiSe2 /Fe3 O4 heterostructures possess high reversible capacities (707...
September 18, 2017: Small
https://www.readbyqxmd.com/read/28922570/catalyst-stability-benchmarking-for-the-oxygen-evolution-reaction-the-importance-of-backing-electrode-material-and-dissolution-in-accelerated-aging-studies
#15
Simon Geiger, Olga Kasian, Andrea M Mingers, Shannon S Nicley, Ken Haenen, Karl J J Mayrhofer, Serhiy Cherevko
In searching for alternative oxygen evolution reaction (OER) catalysts for acidic water splitting, fast screening of materials intrinsic activity and stability in half-cell tests is of vital importance. It significantly accelerates the discovery of new promising materials without the need of time-consuming real cell analysis. In commonly employed tests, a conclusion on the catalyst stability is drawn solely on the basis of pure electrochemical information, e.g. by evaluating potential vs. time profiles. In this work we demonstrate important limitations of such approaches, which are related to the degradation of the backing electrode material...
September 18, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28922550/mesoporous-nanosheet-networked-hybrids-of-cobalt-oxide-and-cobalt-phosphate-for-efficient-electrochemical-and-photoelectrochemical-oxygen-evolution
#16
Bin Liu, Hui-Qing Peng, Cheuk-Nam Ho, Hongtao Xue, Shuilin Wu, Tsz-Wai Ng, Chun-Sing Lee, Wenjun Zhang
A novel mesoporous nanosheet networked hybrid comprising Co3 O4 and Co3 (PO4 )2 is successfully synthesized using a facile and scalable method through calcinating the carbon, cobalt hydroxy carbonate, and cobalt phosphate composite precursor. Electron transfer from Co3 O4 to Co3 (PO4 )2 , together with the special networked structure and the porous nature of the nanosheets enable the Co3 (PO4 )2 -Co3 O4 hybrid to have a high oxygen evolution reaction (OER) activity and outstanding stability in alkaline electrolyte, e...
September 18, 2017: Small
https://www.readbyqxmd.com/read/28922529/borane-stabilized-isomeric-dimers-of-the-phosphaethynolate-anion
#17
Kevin Szkop, Andrew Jupp, Riccardo Suter, Hansjorg Gruetzmacher, Douglas Wade Stephan
The reactions of the phosphaethynolate anion ([PCO]-) with a range of boranes were explored. BPh3 and [PCO]- form a dimeric anion featuring P-B bonds and is prone to dissociation at room temperature. The more Lewis acidic borane B(C6F5)3 yields a less symmetric dimer of [PCO]- with P-B and P-O bonds. Less sterically demanding HB(C6F5)2 and H2B(C6F5) boranes form a third isomer with [PCO]- with both boranes bound to the same phosphorus atom. Despite the unexpected thermodynamic preference for P-coordination, computational data illustrate that electronic and steric features impact the binding modes of the resulting dianionic dimers...
September 18, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28922509/molecular-switching-in-confined-spaces-effects-of-encapsulating-the-dha-vhf-photo-switch-in-cucurbiturils
#18
Michael Å Petersen, Brian Rasmussen, Nicolaj N Andersen, Stephan P A Sauer, Mogens Brøndsted Nielsen, Sophie R Beeren, Michael Pittelkow
Confinement of reactive chemical species uniquely affects chemical reactivity by restricting the physical space available and by restricting access to interactions with the solvent. In Nature, for example, confined protein binding pockets govern processes following photoisomerization reactions and the isomerizations themselves. Here we describe the first example of a dihydroazulene/vinylheptafulvene (DHA/VHF) photo-switch functioning in water, and we show how its switching behavior is strongly influenced by supramolecular interactions with a series of cucurbit[n]uril (CB) host molecules...
September 18, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28922503/modulating-the-structural-properties-of-alpha-gamma-hybrid-peptides-by-alpha-amino-acid-residues-uniform-12-helix-versus-mixed-12-10-helix
#19
Rajkumar Misra, K Muruga Poopathi Raja, Hans-Jörg Hofmann, Hosahudya Gopi
The most important natural a- and 310-helices are stabilized by unidirectional intramolecular hydrogen bonds along the helical cylinder. In contrast, we are reporting here on 12/10-helical conformations with alternately changing H-bond directionality in sequences of alpha,gamma-hybrid peptides [P1: Boc-Ala-Aic-Ala-Aic-COOH; P2: Boc-Leu-Aic-Leu-Aic-OEt; P3: Boc-Leu-Aic-Leu-Aic-Leu-Aic-Aib-OMe; P4: Boc-Ala-Aic-Ala-Aic-Ala-Aic-Ala-OMe; P5: Boc-Leu-Aic-Leu-Aic-Leu-Aic-Leu-Aic-Aib-OMe] composed of natural a-amino acids and the achiral gamma4,4-dimethyl substituted g-amino acid Aic in solution and in single crystals...
September 18, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28922495/formation-of-ni-fe-mixed-diselenide-nanocages-as-a-superior-oxygen-evolution-electrocatalyst
#20
Jianwei Nai, Yan Lu, Le Yu, Xin Wang, Xiong Wen David Lou
Exploring effective electrocatalysts is a crucial requirement for boosting the efficiency of water splitting to obtain clean fuels. Here, a self-templating strategy is reported to synthesize Ni-Fe mixed diselenide cubic nanocages for the electrocatalytic oxygen evolution reaction (OER). The diselenide nanocages are derived from corresponding Prussian-blue analog nanocages, which are first obtained by treating the nanocube precursor with a site-selective ammonia etchant. The resulting Ni-Fe mixed diselenide nanocages perform as a superior OER electrocatalyst, which affords a current density of 10 mA cm(-2) at a small overpotential of 240 mV; a high current density, mass activity, and turnover frequency of 100 mA cm(-2) , 1000 A g(-1) , and 0...
September 18, 2017: Advanced Materials
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