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https://www.readbyqxmd.com/read/29353432/post-translational-modifications-in-tumor-biomarkers-the-next-challenge-for-aptamers
#1
Ana Díaz-Fernández, Rebeca Miranda-Castro, Noemí de-Los-Santos-Álvarez, María Jesús Lobo-Castañón
Advances in proteomics have fueled the search for novel cancer biomarkers with higher selectivity. Differential expression of low abundant proteins has been the usual way of finding those biomarkers. The existence of a selective receptor for each biomarker is compulsory for their use in diagnostic/prognostic assays. Antibodies are the receptors of choice in most cases although aptamers are becoming familiar because of their facile and reproducible synthesis, chemical stability as well as comparable affinity and selectivity...
January 20, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29353431/sensitive-electrochemical-detection-of-microrna-21-based-on-propylamine-functionalized-mesoporous-silica-with-glucometer-readout
#2
Kun Deng, Yong Zhang, Xuedong Tong
A new homogeneous electrochemical sensing system was developed for sensitive detection of microRNA-21 (miRNA-21) based on target-induced glucose release from propylamine-functionalized mesoporous silica nanoparticle (MSN) with glucometer readout. Glucose molecules (as the signal tracers) were initially gated into the pores through the interaction of the negatively charged anchor DNA with the aminated MSN. Upon addition of target miRNA, the analyte competitively hybridized with anchor DNA to form the RNA-DNA duplex, thus resulting in detachment of anchor DNA from the MSN accompanying the pore opening...
January 20, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29353422/biomechanical-in-vitro-comparison-of-suture-anchors-for-thumb-ucl-repair
#3
M Wagner, W Schmoelz, H Stofferin, R Arora
Different types of intraosseous suture anchors can be used for thumb ulnar collateral ligament (UCL) repair surgery. Some of them have already been tested biomechanically in intact bone; however, there exists little knowledge of their stability when implanted in avulsion fracture. In this biomechanical in vitro study, three anchor types (hard, soft and novel BoneWelding) were tested on 24 fresh frozen human thumbs from 12 body donors. After the repair of an iatrogenic ligamentous UCL rupture, the thumbs were cyclically loaded...
January 20, 2018: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/29353415/investigation-of-lactic-acid-bacteria-isolated-from-giant-panda-feces-for-potential-probiotics-in-vitro
#4
Qian Liu, Xueqin Ni, Qiang Wang, Zhirong Peng, Lili Niu, Meiling Xie, Yicen Lin, Yi Zhou, Hao Sun, Kangcheng Pan, Bo Jing, Dong Zeng
The present study aimed to isolate an optimal lactic acid bacterial strain from the feces of healthy giant pandas. The strain exhibited good stability at low pH and high bile salt concentrations, activity against pathogens relevant to pandas, and antibiotic susceptibility. In the current study, 25 isolates were obtained from de Man, Rogosa, and Sharpe agar. Two (E21 and G83) and eight (E1, E2, E16, E18, E21, E69, E70, and G83) isolates demonstrated good performance at pH 2.0 and bile 2% (w/v), respectively...
January 20, 2018: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29353356/generation-of-shrimp-waste-based-dispersant-for-oil-spill-response
#5
Kedong Zhang, Baiyu Zhang, Xing Song, Bo Liu, Liang Jing, Bing Chen
In this study, shrimp waste was enzymatically hydrolyzed to generate a green dispersant and the product was tested for crude oil dispersion in seawater. The hydrolysis process was first optimized based on the dispersant effectiveness (DE) of the product. The functional properties of the product were identified including stability, critical micelle concentration, and emulsification activity. Water was confirmed as a good solvent for dispersant generation when compared with three chemical solvents. The effects of salinity, mixing energy, and temperature on the dispersion of the Alaska North Slope (ANS) crude oil were examined...
January 20, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29353351/the-multiple-roles-of-titin-in-muscle-contraction-and-force-production
#6
REVIEW
Walter Herzog
Titin is a filamentous protein spanning the half-sarcomere, with spring-like properties in the I-band region. Various structural, signaling, and mechanical functions have been associated with titin, but not all of these are fully elucidated and accepted in the scientific community. Here, I discuss the primary mechanical functions of titin, including its accepted role in passive force production, stabilization of half-sarcomeres and sarcomeres, and its controversial contribution to residual force enhancement, passive force enhancement, energetics, and work production in shortening muscle...
January 20, 2018: Biophysical Reviews
https://www.readbyqxmd.com/read/29353324/stromalized-microreactor-supports-murine-hematopoietic-progenitor-enrichment
#7
Danika Khong, Matthew Li, Amy Singleton, Ling-Yee Chin, Biju Parekkadan
There is an emerging need to process, expand, and even genetically engineer hematopoietic stem and progenitor cells (HSPCs) prior to administration for blood reconstitution therapy. A closed-system and automated solution for ex vivo HSC processing can improve adoption and standardize processing techniques. Here, we report a recirculating flow bioreactor where HSCs are stabilized and enriched for short-term processing by indirect fibroblast feeder coculture. Mouse 3 T3 fibroblasts were seeded on the extraluminal membrane surface of a hollow fiber micro-bioreactor and were found to support HSPC cell number compared to unsupported BMCs...
January 20, 2018: Biomedical Microdevices
https://www.readbyqxmd.com/read/29353316/validation-of-a-new-classification-for-periprosthetic-shoulder-fractures
#8
Chlodwig Kirchhoff, Marc Beirer, Ulrich Brunner, Arne Buchholz, Peter Biberthaler, Moritz Crönlein
INTRODUCTION: Successful treatment of periprosthetic shoulder fractures depends on the right strategy, starting with a well-structured classification of the fracture. Unfortunately, clinically relevant factors for treatment planning are missing in the pre-existing classifications. Therefore, the aim of the present study was to describe a new specific classification system for periprosthetic shoulder fractures including a structured treatment algorithm for this important fragility fracture issue...
January 20, 2018: International Orthopaedics
https://www.readbyqxmd.com/read/29353308/single-stage-autotrophic-nitrogen-removal-process-at-high-loading-rate-granular-reactor-performance-kinetics-and-microbial-characterization
#9
Feiyue Qian, Abebe Temesgen Gebreyesus, Jianfang Wang, Yaoliang Shen, Wenru Liu, Lulin Xie
For the possible highest performance of single-stage combined partial nitritation/anammox (PNA) process, a continuous complete-mix granular reactor was operated at progressively higher nitrogen loading rate. The variations in bacterial community structure of granules were also characterized using high-throughput pyrosequencing, to give a detail insight to the relationship between reactor performance and functional organism abundance within completely autotrophic nitrogen removal system. In 172 days of operation, a superior total nitrogen (TN) removal rate over 3...
January 20, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29353276/what-can-google-inform-us-about-people-s-interests-regarding-dental-caries-in-different-populations
#10
Patricia Estefania Aguirre, Melina Coelho, Thaís Oliveira, Daniela Rios, Agnes Fátima Cruvinel, Thiago Cruvinel
The diagnosis or suspicion of dental caries can lead people to seek additional information on the Internet through the use of structured queries in search engine tools. This action generates a considerable volume of data, which can be analyzed to provide a better understanding of the public's behavior linked to the consumption of oral health information. This study aimed to assess the volume and profile of web searches on dental caries-related queries performed by Google users from different countries. The monthly variation of the Search Volume Index (SVI) for dental caries was obtained in Google Trends for the period between January 2004 and September 2016...
January 20, 2018: Caries Research
https://www.readbyqxmd.com/read/29353274/postoperative-immobilization-following-occipitocervical-fusion-in-the-pediatric-population-outcome-evaluation-and-review-of-literature
#11
Alexandre R Pingarilho, Paulo M Porto de Melo, Samer K Elbabaa
The scientific literature does not have a consensus about the role and method of postoperative immobilization after occipitocervical fusion in the pediatric population. The primary goal of this study is to review the medical literature and evaluate different immobilization methods and their impact on fusion, following the surgical management of craniocervical instability in children. It started with an extensive research of randomized controlled trials, series of cases and case reports, describing occipitocervical junction pathologies, clinical, epidemiological characteristics, and treatment...
January 20, 2018: Pediatric Neurosurgery
https://www.readbyqxmd.com/read/29353198/a-tiered-approach-to-assess-effects-of-diclofenac-on-the-brown-mussel-perna-perna-a-contribution-to-characterize-the-hazard
#12
Mayana Karoline Fontes, Paloma Kachel Gusso-Choueri, Luciane Alves Maranho, Denis Moledo de Souza Abessa, Wesley Almeida Mazur, Bruno Galvão de Campos, Luciana Lopes Guimarães, Marcos Sergio de Toledo, Daniel Lebre, Joyce Rodrigues Marques, Andreia Arantes Felicio, Augusto Cesar, Eduardo Alves Almeida, Camilo Dias Seabra Pereira
Pharmaceutical discharges into the aquatic ecosystem are of environmental concern and sewage treatment plants (STPs) have been pointed out as the major source of these compounds to coastal zones, where oceanic disposal of sewage occurs through submarine outfalls. Diclofenac (DCF) is one of the most frequently detected pharmaceuticals in water, but little is known about the effects on marine organisms. In this study, we employed a tiered approach involving the determination of environmental concentrations of DCF in marine water and the adverse biological effects for fertilization, embryo-larval development and biomarker responses of the mussel Perna perna...
December 29, 2017: Water Research
https://www.readbyqxmd.com/read/29353196/high-adsorption-of-methylene-blue-by-salicylic-acid-methanol-modified-steel-converter-slag-and-evaluation-of-its-mechanism
#13
Min Cheng, Guangming Zeng, Danlian Huang, Cui Lai, Yang Liu, Chen Zhang, Rongzhong Wang, Lei Qin, Wenjing Xue, Biao Song, Shujing Ye, Huan Yi
A novel adsorbent based on steel converter slag (SCS), useful for adsorbing cationic pollutants from water was prepared by a simple method. The characterization showed that salicylic acid-methanol (SAM) modification selectively removed calcium silicate minerals from the surface of SCS and lead to a prominent increase in the specific surface areas. The maximum adsorption capacity of SAM-modified SCS for methylene blue (MB) at initial pH of 7.0 and temperature of 293 K was 41.62 mg/g, which is 35.2-times higher than that of SCS (1...
January 3, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29353189/synthesis-of-cross-linked-protein-metal-hybrid-nanoflowers-and-its-application-in-repeated-batch-decolorization-of-synthetic-dyes
#14
Sanjay K S Patel, Sachin V Otari, Jinglin Li, Dong Rip Kim, Sun Chang Kim, Byung-Kwan Cho, Vipin C Kalia, Yun Chan Kang, Jung-Kul Lee
Herein, we report the preparation of a cross-linked protein-metal hybrid nanoflower (NF) system for laccase immobilization. The immobilized laccase showed effective encapsulation yield and activity recovery of 78.1% and 204%, respectively. The catalytic efficiency (kcatVmax-1) of cross-linked NF (CL-NF) was 2.2-fold more than that of free laccase. The CL-NF also exhibited significantly higher stability towards pH and temperature changes. It exhibited excellent storage stability and tolerance towards solvents and inhibitors as compared with the free enzyme...
January 4, 2018: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29353188/mechanism-of-anaerobic-bio-reduction-of-azo-dye-assisted-with-lawsone-immobilized-activated-carbon
#15
Daniel Olivo-Alanis, Refugio Bernardo Garcia-Reyes, Luis H Alvarez, Alcione Garcia-Gonzalez
Lawsone redox (LQ) mediator was covalently bound to granular activated carbon (GAC) by Fischer esterification. A high LQ adsorption capacity on GAC was achieved (∼230 mg/g), and desorption studies showed strong chemical stability. Furthermore, kinetic experiments with solid-phase redox mediator (RM) and their controls (soluble RM, GAC and anaerobic sludge) were tested for decolorization of congo red dye at initial concentration of 175 mg/L. Benzidine, a by-product of complete congo red reduction, was also measured by HPLC analysis along the kinetic experiments...
January 9, 2018: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29353183/automatic-bad-channel-detection-in-intracranial-electroencephalographic-recordings-using-ensemble-machine-learning
#16
Viateur Tuyisenge, Lena Trebaul, Manik Bhattacharjee, Blandine Chanteloup-Forêt, Carole Saubat-Guigui, Ioana Mîndruţă, Sylvain Rheims, Louis Maillard, Philippe Kahane, Delphine Taussig, Olivier David
OBJECTIVE: Intracranial electroencephalographic (iEEG) recordings contain "bad channels", which show non-neuronal signals. Here, we developed a new method that automatically detects iEEG bad channels using machine learning of seven signal features. METHODS: The features quantified signals' variance, spatial-temporal correlation and nonlinear properties. Because the number of bad channels is usually much lower than the number of good channels, we implemented an ensemble bagging classifier known to be optimal in terms of stability and predictive accuracy for datasets with imbalanced class distributions...
December 24, 2017: Clinical Neurophysiology: Official Journal of the International Federation of Clinical Neurophysiology
https://www.readbyqxmd.com/read/29353171/microcystin-lr-nanobody-screening-from-an-alpaca-phage-display-nanobody-library-and-its-expression-and-application
#17
Chongxin Xu, Ying Yang, Liwen Liu, Jianhong Li, Xiaoqin Liu, Xiao Zhang, Yuan Liu, Cunzheng Zhang, Xianjin Liu
Microcystin-LR (MC-LR) is a type of biotoxin that pollutes the ecological environment and food. The study aimed to obtain new nanobodies from phage nanobody library for determination of MC-LR. The toxin was conjugated to keyhole limpet haemocyanin (KLH) and bovine serum albumin (BSA), respectively, then the conjugates were used as coated antigens for enrichment (coated MC-LR-KLH) and screening (coated MC-LR-BSA) of MC-LR phage nanobodies from an alpaca phage display nanobody library. The antigen-specific phage particles were enriched effectively with four rounds of biopanning...
January 17, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29353168/zinc-and-lead-detoxifying-abilities-of-humic-substances-relevant-to-environmental-bacterial-species
#18
L V Perelomov, Binoy Sarkar, O I Sizova, K B Chilachava, A Y Shvikin, I V Perelomova, Y M Atroshchenko
The effect of humic substances (HS) and their different fractions (humic acids (HA) and hymatomelanic acids (HMA)) on the toxicity of zinc and lead to different strains of bacteria was studied. All tested bacteria demonstrated a lower resistance to zinc than lead showing minimum inhibitory concentrations of 0.1 - 0.3mM and 0.3-0.5mM, respectively. The highest resistance to lead was characteristic of Pseudomonas chlororaphis PCL1391 and Rhodococcus RS67, while Pseudomonas chlororaphis PCL1391 showed the greatest resistance to zinc...
January 17, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29353162/monolayers-of-a-tetrazine-containing-gemini-amphiphile-interplays-with-biomembrane-lipids
#19
Hiromichi Nakahara, Masayori Hagimori, Takahiro Mukai, Osamu Shibata
The property of a newly synthesized tetrazine derivative comprised of double C18-saturated hydrocarbon chain (C18-rTz-C18) has been studied in situ at the air-water interface. C18-rTz-C18 or a gemini amphiphile contributes to restriction of its tetrazine moiety on the interface, which is expected to be used for bioimaging and analytical reagents. Herein, to understand lateral interactions between Tz and biomembrane constituents, we investigated the interfacial behavior of Langmuir monolayers composed of C18-rTz-C18 and biomembrane lipids such as DPPC, DPPG, DPPE, PSM, and Cholesterol (Ch)...
January 17, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29353113/cadmium-immobilization-in-river-sediment-using-stabilized-nanoscale-zero-valent-iron-with-enhanced-transport-by-polysaccharide-coating
#20
Danlian Huang, Zhengxun Hu, Zhiwei Peng, Guangming Zeng, Guomin Chen, Chen Zhang, Min Cheng, Jia Wan, Xi Wang, Xiang Qin
Proper management of metal-contaminated sediment plays a key role in sediment recovery and reuse. This study synthesized two kinds of stabilized nanoscale zero-valent iron (nZVI) with starch (S-nZVI) and carboxymethyl cellulose (C-nZVI) for the in situ immobilization of Cd(II) in river sediment and investigated their transport in porous media. Experimental data showed that when the sediment sample was treated with C-nZVI for 56 days at a dosage ranging from 5 to 10 mg/g-sediment as Fe0, the TCLP (toxicity characteristic leaching procedure) leachability of Cd(II) in the sediment decreased by 93...
January 17, 2018: Journal of Environmental Management
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