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https://www.readbyqxmd.com/read/27830561/report-on-liver-cell-transplantation-using-human-fetal-liver-cells
#1
Giada Pietrosi, Cinzia Chinnici
In an era of organ shortage, human fetuses donated after medically indicated abortion could be considered a potential liver donor for hepatic cell isolation. We investigated transplantation of fetal liver cells as a strategy to support liver functionality in end-stage liver disease. Here, we report our protocol of human fetal liver cells (hFLC) isolation in fetuses from 17 to 22 gestational weeks, and our clinical procedure of hFLC transplantation through the splenic artery.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27737933/the-membrane-scaffold-slp2-anchors-a-proteolytic-hub-in-mitochondria-containing-parl-and-the-i-aaa-protease-yme1l
#2
Timothy Wai, Shotaro Saita, Hendrik Nolte, Sebastian Müller, Tim König, Ricarda Richter-Dennerlein, Hans-Georg Sprenger, Joaquin Madrenas, Mareike Mühlmeister, Ulrich Brandt, Marcus Krüger, Thomas Langer
The SPFH (stomatin, prohibitin, flotillin, HflC/K) superfamily is composed of scaffold proteins that form ring-like structures and locally specify the protein-lipid composition in a variety of cellular membranes. Stomatin-like protein 2 (SLP2) is a member of this superfamily that localizes to the mitochondrial inner membrane (IM) where it acts as a membrane organizer. Here, we report that SLP2 anchors a large protease complex composed of the rhomboid protease PARL and the i-AAA protease YME1L, which we term the SPY complex (for SLP2-PARL-YME1L)...
December 2016: EMBO Reports
https://www.readbyqxmd.com/read/27094329/function-of-the-borrelia-burgdorferi-ftsh-homolog-is-essential-for-viability-both-in-vitro-and-in-vivo-and-independent-of-hflk-c
#3
Chen-Yi Chu, Philip E Stewart, Aaron Bestor, Bryan Hansen, Tao Lin, Lihui Gao, Steven J Norris, Patricia A Rosa
UNLABELLED: In many bacteria, the FtsH protease and its modulators, HflK and HflC, form a large protein complex that contributes to both membrane protein quality control and regulation of the cellular response to environmental stress. Both activities are crucial to the Lyme disease pathogen Borrelia burgdorferi, which depends on membrane functions, such as motility, protein transport, and cell signaling, to respond to rapid changes in its environment. Using an inducible system, we demonstrate that FtsH production is essential for both mouse and tick infectivity and for in vitro growth of B...
2016: MBio
https://www.readbyqxmd.com/read/27001260/differing-effects-of-high-fat-or-high-carbohydrate-meals-on-food-hedonics-in-overweight-and-obese-individuals
#4
RANDOMIZED CONTROLLED TRIAL
Mark Hopkins, Catherine Gibbons, Phillipa Caudwell, John E Blundell, Graham Finlayson
Although the effects of dietary fat and carbohydrate on satiety are well documented, little is known about the impact of these macronutrients on food hedonics. We examined the effects of ad libitum and isoenergetic meals varying in fat and carbohydrate on satiety, energy intake and food hedonics. In all, sixty-five overweight and obese individuals (BMI=30·9 (sd 3·8) kg/m2) completed two separate test meal days in a randomised order in which they consumed high-fat/low-carbohydrate (HFLC) or low-fat/high-carbohydrate (LFHC) foods...
May 28, 2016: British Journal of Nutrition
https://www.readbyqxmd.com/read/26333341/new-parameters-available-on-sysmex-xe-5000-hematology-analyzers-contribute-to-differentiating-dengue-from-leptospirosis-and-enteric-fever
#5
A Oehadian, M Michels, Q de Mast, D Prihatni, M Puspita, Y Hartantri, S Sinarta, A J A M van der Ven, B Alisjahbana
INTRODUCTION: Distinguishing dengue virus infection from other febrile thrombocytopenic illnesses such as leptospirosis or enteric fever is important but difficult, due to the unavailability of reliable diagnostic tests. Sysmex XE-5000 hematology analyzers use fluorescence flow cytometry to quantitate new parameters including cells in the atypical lymphocyte area (AL), high-fluorescent lymphocyte counts (HFLC), immature granulocytes (IG), and immature platelets (IPF). This study aimed to investigate whether these parameters can help to discriminate between the diseases...
December 2015: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/26067063/narj-subfamily-system-specific-chaperone-diversity-and-evolution-is-directed-by-respiratory-enzyme-associations
#6
Denice C Bay, Catherine S Chan, Raymond J Turner
BACKGROUND: Redox enzyme maturation proteins (REMPs) describe a diverse family of prokaryotic chaperones involved in the biogenesis of anaerobic complex iron sulfur molybdoenzyme (CISM) respiratory systems. Many REMP family studies have focused on NarJ subfamily members from Escherichia coli: NarJ, NarW, DmsD, TorD and YcdY. The aim of this bioinformatics study was to expand upon the evolution, distribution and genetic association of these 5 REMP members within 130 genome sequenced taxonomically diverse species representing 324 Prokaryotic sequences...
2015: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/25731940/functions-that-protect-escherichia-coli-from-dna-protein-crosslinks
#7
Rachel Krasich, Sunny Yang Wu, H Kenny Kuo, Kenneth N Kreuzer
Pathways for tolerating and repairing DNA-protein crosslinks (DPCs) are poorly defined. We used transposon mutagenesis and candidate gene approaches to identify DPC-hypersensitive Escherichia coli mutants. DPCs were induced by azacytidine (aza-C) treatment in cells overexpressing cytosine methyltransferase; hypersensitivity was verified to depend on methyltransferase expression. We isolated hypersensitive mutants that were uncovered in previous studies (recA, recBC, recG, and uvrD), hypersensitive mutants that apparently activate phage Mu Gam expression, and novel hypersensitive mutants in genes involved in DNA metabolism, cell division, and tRNA modification (dinG, ftsK, xerD, dnaJ, hflC, miaA, mnmE, mnmG, and ssrA)...
April 2015: DNA Repair
https://www.readbyqxmd.com/read/24845470/high-resolution-clear-native-electrophoresis-is-a-good-alternative-to-blue-native-electrophoresis-for-the-characterization-of-the-escherichia-coli-membrane-complexes
#8
COMPARATIVE STUDY
Ernesto Diéguez-Casal, Paula Freixeiro, Liliana Costoya, M Teresa Criado, Carlos Ferreirós, Sandra Sánchez
Blue native electrophoresis (BNE) has become the most popular method for the global analysis of membrane protein complexes. Although it has been shown to be very useful for that purpose, it can produce the dissociation of complexes with weak interactions and, due to the use of Coomassie Brilliant Blue, does not allow the subsequent application of fluorimetric and/or enzymatic techniques. Recently, we have successfully used the high resolution clear native electrophoresis (hrCNE) for the analysis of Neisseria meningitidis outer membrane porin complexes...
July 2014: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/24395719/novel-parameters-of-extended-complete-blood-cell-count-under-fluorescence-flow-cytometry-in-patients-with-sepsis
#9
Borros M Arneth, Maximilian Ragaller, Kathleen Hommel, Oliver Tiebel, Mario Menschikowski, Gabriele Siegert
BACKGROUND: We first describe a patient who developed urosepsis from an ordinary urinary tract infection. In this case, the new hematological parameters of immature leukocytes, that is, the high-fluorescence lymphocyte cell (HFLC) and immature granulocyte (IG) counts peaked early, whereas the established infection parameters, that is, C-reactive protein (CRP) and total white blood cell count showed less dynamic regarding infection and therapy. METHODS: To investigate this phenomenon in greater detail, the novel parameters HFLC and IG counts are investigated retrospectively in a cohort of 38 patients who were admitted to the anesthesia intensive care unit...
March 2014: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/24075505/consuming-a-hypocaloric-high-fat-low-carbohydrate-diet-for-12-weeks-lowers-c-reactive-protein-and-raises-serum-adiponectin-and-high-density-lipoprotein-cholesterol-in-obese-subjects
#10
RANDOMIZED CONTROLLED TRIAL
Megan R Ruth, Ava M Port, Mitali Shah, Ashley C Bourland, Nawfal W Istfan, Kerrie P Nelson, Noyan Gokce, Caroline M Apovian
OBJECTIVE: High fat, low carbohydrate (HFLC) diets have become popular tools for weight management. We sought to determine the effects of a HFLC diet compared to a low fat high carbohydrate (LFHC) diet on the change in weight loss, cardiovascular risk factors and inflammation in subjects with obesity. METHODS: Obese subjects (29.0-44.6 kg/m2) recruited from Boston Medical Center were randomized to a hypocaloric LFHC (n=26) or HFLC (n=29) diet for 12 weeks. RESULTS: The age range of subjects was 21-62 years...
December 2013: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/23935682/effect-of-dietary-cocoa-tea-camellia-ptilophylla-supplementation-on-high-fat-diet-induced-obesity-hepatic-steatosis-and-hyperlipidemia-in-mice
#11
Xiao Rong Yang, Elaine Wat, Yan Ping Wang, Chun Hay Ko, Chi Man Koon, Wing Sum Siu, Si Gao, David Wing Shing Cheung, Clara Bik San Lau, Chuang Xing Ye, Ping Chung Leung
Recent studies suggested that green tea has the potential to protect against diet-induced obesity. The presence of caffeine within green tea has caused limitations. Cocoa tea (Camellia ptilophylla) is a naturally decaffeinated tea plant. To determine whether cocoa tea supplementation results in an improvement in high-fat diet-induced obesity, hyperlipidemia and hepatic steatosis, and whether such effects would be comparable to those of green tea extract, we studied six groups (n = 10) of C57BL/6 mice that were fed with (1) normal chow (N); (2) high-fat diet (21% butterfat + 0...
2013: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/23188627/clinical-value-of-a-high-fat-and-low-carbohydrate-diet-before-fdg-pet-ct-for-evaluation-of-patients-with-suspected-cardiac-sarcoidosis
#12
Michael Soussan, Pierre-Yves Brillet, Hilario Nunes, Gabriel Pop, Matthieu-John Ouvrier, Nicolas Naggara, Dominique Valeyre, Pierre Weinmann
BACKGROUND: We hypothesized that a high-fat and low-carbohydrate (HFLC) diet before FDG-PET/CT could identify patients with active cardiac sarcoidosis (CS). METHODS: Fifty-eight sarcoidosis patients with a suspicion of CS consumed a HFLC diet before FDG-PET/CT. Clinical, electrical, and other imaging investigations were compared to PET results. RESULTS: Using Japanese Ministry of Health and Welfare (JMHW) criteria as a gold standard, 21% (12/58) of patients had a CS...
February 2013: Journal of Nuclear Cardiology: Official Publication of the American Society of Nuclear Cardiology
https://www.readbyqxmd.com/read/22753055/synthetic-motility-and-cell-shape-defects-associated-with-deletions-of-flotillin-reggie-paralogs-in-bacillus-subtilis-and-interplay-of-these-proteins-with-nfed-proteins
#13
Felix Dempwolff, Heiko M Möller, Peter L Graumann
Flotillin/reggie proteins are membrane-associated proteins present in all kinds of cells and belong to the family of proteins carrying the SPFH (stomatin, prohibitin, flotillin, and HflK/HflC) domain. In addition to this domain of unknown function, flotillin proteins are characterized by the flotillin domain, which is rich in heptad repeats. Bacterial flotillin orthologs have recently been shown to be part of lipid rafts, like their eukaryotic counterparts, and to be involved in signaling events. Double deletions of floT and the gene encoding the second flotillin-like protein in Bacillus subtilis, floA, show strong synthetic defects in cell morphology, motility, and transformation efficiency...
September 2012: Journal of Bacteriology
https://www.readbyqxmd.com/read/22623988/mitochondrial-and-plasma-membrane-pools-of-stomatin-like-protein-2-coalesce-at-the-immunological-synapse-during-t-cell-activation
#14
Darah A Christie, Mark G Kirchhof, Santosh Vardhana, Michael L Dustin, Joaquín Madrenas
Stomatin-like protein 2 (SLP-2) is a member of the stomatin-prohibitin-flotillin-HflC/K (SPFH) superfamily. Recent evidence indicates that SLP-2 is involved in the organization of cardiolipin-enriched microdomains in mitochondrial membranes and the regulation of mitochondrial biogenesis and function. In T cells, this role translates into enhanced T cell activation. Although the major pool of SLP-2 is associated with mitochondria, we show here that there is an additional pool of SLP-2 associated with the plasma membrane of T cells...
2012: PloS One
https://www.readbyqxmd.com/read/22144107/expression-of-paramyxovirus-v-proteins-promotes-replication-and-spread-of-hepatitis-c-virus-in-cultures-of-primary-human-fetal-liver-cells
#15
Linda Andrus, Svetlana Marukian, Christopher T Jones, Maria Teresa Catanese, Timothy P Sheahan, John W Schoggins, Walter T Barry, Lynn B Dustin, Kartik Trehan, Alexander Ploss, Sangeeta N Bhatia, Charles M Rice
UNLABELLED: Here we demonstrate that primary cultures of human fetal liver cells (HFLC) reliably support infection with laboratory strains of hepatitis C virus (HCV), although levels of virus replication vary significantly between different donor cell preparations and frequently decline in a manner suggestive of active viral clearance. To investigate possible contributions of the interferon (IFN) system to control HCV infection in HFLC, we exploited the well-characterized ability of paramyxovirus (PMV) V proteins to counteract both IFN induction and antiviral signaling...
December 2011: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/21885848/the-cholesterol-content-of-western-diets-plays-a-major-role-in-the-paradoxical-increase-in-high-density-lipoprotein-cholesterol-and-upregulates-the-macrophage-reverse-cholesterol-transport-pathway
#16
Joan Carles Escolà-Gil, Gemma Llaverias, Josep Julve, Matti Jauhiainen, Jesús Méndez-González, Francisco Blanco-Vaca
OBJECTIVE: A high-saturated fatty acid- and cholesterol-containing (HFHC) diet is considered to be a major risk factor for cardiovascular disease. The present study aimed to determine the effects of this Western-type diet on high-density lipoprotein (HDL) metabolism and reverse cholesterol transport (RCT) from macrophages to feces. METHODS AND RESULTS: Experiments were carried out in mice fed a low-fat, low-cholesterol diet, an HFHC diet, or an HFHC diet without added cholesterol (high-saturated fatty acid and low-cholesterol [HFLC])...
November 2011: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/21800339/hepatitis-c-virus-induces-interferon-%C3%AE-and-interferon-stimulated-genes-in-primary-liver-cultures
#17
Svetlana Marukian, Linda Andrus, Timothy P Sheahan, Christopher T Jones, Edgar D Charles, Alexander Ploss, Charles M Rice, Lynn B Dustin
UNLABELLED: Hepatitis C virus (HCV) replication in primary liver cells is less robust than that in hepatoma cell lines, suggesting that innate antiviral mechanisms in primary cells may limit HCV replication or spread. Here we analyzed the expression of 47 genes associated with interferon (IFN) induction and signaling following HCV infection of primary human fetal liver cell (HFLC) cultures from 18 different donors. We report that cell culture-produced HCV (HCVcc) induced expression of Type III (λ) IFNs and of IFN-stimulated genes (ISGs)...
December 2011: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/21764915/membrane-proteases-and-aminoglycoside-antibiotic-resistance
#18
Aaron Hinz, Samuel Lee, Kyle Jacoby, Colin Manoil
We present genetic studies that help define the functional network underlying intrinsic aminoglycoside resistance in Pseudomonas aeruginosa. Our analysis shows that proteolysis, particularly that controlled by the membrane protease FtsH, is a major determinant of resistance. First, we examined the consequences of inactivating genes controlled by AmgRS, a two-component regulator required for intrinsic tobramycin resistance. Three of the gene products account for resistance: a modulator of FtsH protease (YccA), a membrane protease (HtpX), and a membrane protein of unknown function (PA5528)...
September 2011: Journal of Bacteriology
https://www.readbyqxmd.com/read/21497606/characterization-of-the-consequences-of-yidc-depletion-on-the-inner-membrane-proteome-of-e-coli-using-2d-blue-native-sds-page
#19
David Wickström, Samuel Wagner, Per Simonsson, Ovidiu Pop, Louise Baars, A Jimmy Ytterberg, Klaas J van Wijk, Joen Luirink, Jan-Willem L de Gier
In the bacterium Escherichia coli, the essential inner membrane protein (IMP) YidC assists in the biogenesis of IMPs and IMP complexes. Our current ideas about the function of YidC are based on targeted approaches using only a handful of model IMPs. Proteome-wide approaches are required to further our understanding of the significance of YidC and to find new YidC substrates. Here, using two-dimensional blue native/SDS-PAGE methodology that is suitable for comparative analysis, we have characterized the consequences of YidC depletion for the steady-state levels and oligomeric state of the constituents of the inner membrane proteome...
June 3, 2011: Journal of Molecular Biology
https://www.readbyqxmd.com/read/21361853/sensitivity-and-specificity-of-the-high-fluorescent-lymphocyte-count-gate-on-the-sysmex-xe-5000-hematology-analyzer-for-detection-of-peripheral-plasma-cells
#20
Edwin van Mirre, Gert Jan Vrielink, Nicole Tjon-a-Tsoi, Henriët Hendriks, Wim de Kieviet, Edwin ten Boekel
BACKGROUND: Plasma cells are one of the end products of the B-lymphocyte mediated immune response. These cells normally reside in the bone marrow or some peripheral lymphoid tissues. Increased numbers of plasma cells in the blood, usually indicates pathology such as infection, auto-immunity or haematological malignancy. Therefore, the ability to measure plasma cells (PCs) on an automated cell analyzer might be advantageous. METHODS: The performance of the Sysmex XE-5000 leukocyte differential channel [high fluorescent lymphocyte count (HFLC) area] was evaluated for the ability to detect plasma cells in peripheral blood and compared to the detection of plasma cells by flow cytometric analyses...
April 2011: Clinical Chemistry and Laboratory Medicine: CCLM
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