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https://www.readbyqxmd.com/read/29151158/guidelines-to-reach-high-quality-purified-recombinant-proteins
#1
REVIEW
Carla Oliveira, Lucília Domingues
The final goal in recombinant protein production is to obtain high-quality pure protein samples. Indeed, the successful downstream application of a recombinant protein depends on its quality. Besides production, which is conditioned by the host, the quality of a recombinant protein product relies mainly on the purification procedure. Thus, the purification strategy must be carefully designed from the molecular level. On the other hand, the quality control of a protein sample must be performed to ensure its purity, homogeneity and structural conformity, in order to validate the recombinant production and purification process...
November 18, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29151074/interferon-lambda-2-promotes-mammary-tumor-metastasis-via-angiogenesis-extension-and-stimulation-of-cancer-cell-migration
#2
R Pingwara, K Witt-Jurkowska, K Ulewicz, J Mucha, K Tonecka, Z Pilch, B Taciak, K Zabielska-Koczywas, M Mori, S Berardozzi, B Botta, T P Rygiel, M Krol
Myeloid-derived suppressor cells (MDSCs) support tumor development by stimulation of angiogenesis and immune response inhibition. In our previous study, we showed that interferon lambda 2 (IFN-λ2), secreted by MDSCs, enhances production of pro-angiogenic factors by cancer cells via phosphorylation of STAT3 and therefore promotes blood vessels formation. In the present study IFN-λ2 level was evaluated by ELISA in serum of tumor-bearing mice, whereas its expression in MDSCs isolated from the lungs with metastatic tumors and normal lungs was assessed by qPCR...
August 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/29151026/regional-validation-and-recalibration-of-clinical-predictive-models-for-patients-with-acute-heart-failure
#3
Benjamin S Wessler, Robin Ruthazer, James E Udelson, Mihai Gheorghiade, Faiez Zannad, Aldo Maggioni, Marvin A Konstam, David M Kent
BACKGROUND: Heart failure clinical practice guidelines recommend applying validated clinical predictive models (CPMs) to support decision making. While CPMs are now widely available, the generalizability of heart failure CPMs is largely unknown. METHODS AND RESULTS: We identified CPMs derived in North America that predict mortality for patients with acute heart failure and validated these models in different world regions to assess performance in a contemporary international clinical trial (N=4133) of patients with acute heart failure treated with guideline-directed medical therapy...
November 18, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/29151007/sagittal-pelvic-orientation-a-comparison-of-two-methods-of-measurement
#4
Aaron Buckland, Edward DelSole, Stephen George, Shaleen Vira, Virginie Lafage, Thomas Errico, Jonathan Vigdorchik
Pelvic tilt is an essential parameter in spinal deformity surgery and in acetabular positioning for total hip arthroplasty. However, the measurement of tilt varies between the hip and spine literature. Hip surgeons measure the anterior pelvic plane tilt, whereas spine surgeons measure the spinopelvic tilt. This study uses stereoradiography (EOS imaging SA, Paris, France) to assess the relationship and the inter-observer and intra-observer reliability of measuring these two common references for pelvic tilt...
December 2017: Bulletin of the Hospital for Joint Diseases
https://www.readbyqxmd.com/read/29150976/salt-bridge-interactions-within-the-%C3%AE-2-integrin-%C3%AE-7-helix-mediate-force-induced-binding-and-shear-resistance-ability
#5
Xiao Zhang, Linda Li, Ning Li, Xinyu Shu, Lüwen Zhou, Shouqin Lü, Shenbao Chen, Debin Mao, Mian Long
The functional performance of αI domain α7 helix in β2 integrin activation depends on the allostery of the α7 helix, which axially slides down; therefore, it is critical to elucidate what factors regulate the allostery. In this study, we determined that there were two conservative salt bridge interaction pairs that constrain both the upper and bottom ends of the α7 helix. Molecular Dynamics (MD) simulations for three β2 integrin members of LFA-1 (αL β2 ), Mac-1 (αM β2 ) and αx β2 indicated that the magnitude of the salt bridge interaction is related to the stability of the αI domain and the strength of the corresponding force-induced allostery...
November 18, 2017: FEBS Journal
https://www.readbyqxmd.com/read/29150925/one-pot-synthesis-of-a-multi-template-molecularly-imprinted-polymer-for-the-extraction-of-six-sulfonamide-residues-from-milk-before-hplc-with-diode-array-detection
#6
Maria Kechagia, Victoria Samanidou, Abuzar Kabir, Kenneth G Furton
A highly selective molecularly imprinted polymer sorbent was synthesized and employed for the simultaneous determination of six sulfonamide antibiotic residues (sulfanilamide, sulfacetamide, sulfadiazine, sulfathiazole, sulfamerazine and sulfamethizole) in milk samples. Multi-analyte imprinted particles were used as sorbent in solid phase extraction. Sulfonamides were separated on a high-performance liquid chromatography column (Merck-Lichrospher RP18e, 5 μm 250 × 4 mm) and further identified and quantified by diode array detection...
November 18, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/29150898/a-model-to-characterize-psychopathological-features-in-adults-with-prader-willi-syndrome
#7
Denise Thuilleaux, Virginie Laurier, Pierre Copet, Julie Tricot, Geneviève Demeer, Fabien Mourre, Maithé Tauber, Joseba Jauregi
High prevalence of behavioral and psychiatric disorders in adults with Prader-Willi Syndrome (PWS) has been reported in last few years. However, data are confusing and often contradictory. In this article, we propose a model to achieve a better understanding of the psychopathological features in adults with PWS. The study is based on clinical observations of 150 adult inpatients, males and females. Non-parametric statistics were performed to analyse the association of psychopathological profiles with genotype, gender and age...
November 17, 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/29150884/directional-local-field-potentials-a-tool-to-optimize-deep-brain-stimulation
#8
Gerd Tinkhauser, Alek Pogosyan, Ines Debove, Andreas Nowacki, Syed Ahmar Shah, Kathleen Seidel, Huiling Tan, John-Stuart Brittain, Katrin Petermann, Lazzaro di Biase, Markus Oertel, Claudio Pollo, Peter Brown, Michael Schuepbach
BACKGROUND: Although recently introduced directional DBS leads provide control of the stimulation field, programing is time-consuming. OBJECTIVES: Here, we validate local field potentials recorded from directional contacts as a predictor of the most efficient contacts for stimulation in patients with PD. METHODS: Intraoperative local field potentials were recorded from directional contacts in the STN of 12 patients and beta activity compared with the results of the clinical contact review performed after 4 to 7 months...
November 18, 2017: Movement Disorders: Official Journal of the Movement Disorder Society
https://www.readbyqxmd.com/read/29150698/surgical-techniques-for-left-atrial-appendage-exclusion
#9
REVIEW
Sacha P Salzberg, Maximilian Y Emmert, Etem Caliskan
The increasing prevalence of atrial fibrillation with the aging population and its associated major morbidity and mortality due to thromboembolic stroke have resulted in intensive research on stroke prevention or stroke risk reduction strategies. Several surgical techniques for left atrial appendage (LAA) occlusion have evolved over the past decades. Surgeons have been using different techniques leading to highly variable and, in particular, poor data on outcomes. LAA closure is performed either as a concomitant procedure during open-heart surgery or as a stand-alone surgical procedure as part of minimally invasive (mini-thoracotomy or thoracoscopy) arrhythmia surgery...
November 17, 2017: Herzschrittmachertherapie & Elektrophysiologie
https://www.readbyqxmd.com/read/29150246/development-of-the-autosomal-dominant-polycystic-kidney-disease-impact-scale-a-new-health-related-quality-of-life-instrument
#10
Dorothee Oberdhan, Jason C Cole, Holly B Krasa, Rebecca Cheng, Frank S Czerwiec, Ron D Hays, Arlene B Chapman, Ronald D Perrone
BACKGROUND: The impact of autosomal dominant polycystic kidney disease (ADPKD) on health-related quality of life (HRQoL) is not well understood due to a lack of instruments specific to the condition. STUDY DESIGN: Content for a new self-administered patient-reported outcome (PRO) questionnaire to assess ADPKD-related HRQoL was developed through clinical expert and patient focus group discussions. The new PRO instrument was administered to study patients with ADPKD to evaluate its reliability and validity...
November 14, 2017: American Journal of Kidney Diseases: the Official Journal of the National Kidney Foundation
https://www.readbyqxmd.com/read/29150094/-geriatric-assessment-and-prognostic-scores-in-older-cancer-patient-additional-support-to-the-therapeutic-decision
#11
REVIEW
Frédéric Pamoukdjian, Evelyne Liuu, Philippe Caillet, Mathilde Gisselbrecht, Stéphane Herbaud, Pascaline Boudou-Rouquette, Laurent Zelek, Elena Paillaud
Cancer is a disease of the elderly as demonstrated by the epidemiological evolution of Western countries. Indeed, two third of cancers newly diagnosed occur over 65 years. However, older cancer patients have been often excluded from clinical trials in oncology and the extrapolation of cancer treatments in this population remains difficult in practice. Scientific societies recommend that a comprehensive geriatric assessment (CGA) be performed in patients aged 70 and over and selected using screening tools for frailty such as the G8 index...
November 14, 2017: Bulletin du Cancer
https://www.readbyqxmd.com/read/29150091/prediction-of-menarcheal-status-of-girls-using-voice-features
#12
Marcin D Bugdol, Monika N Bugdol, Anna M Lipowicz, Andrzej W Mitas, Maria J Bienkowska, Agata M Wijata
A method for evaluating the menarcheal status of girls on the basis of their voice features is presented in the paper. The registration procedure consists of voice recording and measuring 20 anthropological features. The input feature vector is a combination of voice and anthropometric parameters, counting 220 features. The optimal set of parameters was selected using five different methods: Method A - stepwise regression (first forward, then backward regression) performed on features with statistically different means/medians; Method B - stepwise regression (forward and backward) on all features, with age; Method C - stepwise regression as in B; including age, Method D - all features with statistically different means/medians, Method E - all features excluding age...
November 8, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/29150007/crispr-in-animals-and-animal-models
#13
Ellen Shrock, Marc Güell
CRISPR-Cas9 has revolutionized the generation of transgenic animals. This system has demonstrated an unprecedented efficiency, multiplexability, and ease of use, thereby reducing the time and cost required for genome editing and enabling the production of animals with more extensive genetic modifications. It has also been shown to be applicable to a wide variety of animals, from early-branching metazoans to primates. Genome-wide screens in model organisms have been performed, accurate models of human diseases have been constructed, and potential therapies have been tested and validated in animal models...
2017: Progress in Molecular Biology and Translational Science
https://www.readbyqxmd.com/read/29149972/right-extended-split-liver-transplantation-compared-with-whole-liver-transplantation-lessons-learned-at-a-single-center-in-latin-america-results-from-a-match-case-control-study
#14
S E Gambaro, P Romero, N Pedraza, L Moulin, S Yantorno, D Ramisch, C Rumbo, P Barros-Schelotto, V Descalzi, G E Gondolesi
BACKGROUND: Despite the progressively increasing gap between patients waiting for liver transplant under the Model for End-stage Liver Disease MELD system and the availability of deceased donor organs, the use of right extended split liver grafts (RESLG) has not been accepted by all centers. In this study, we compared the results obtained using RESLG vs a group of matched whole liver graft (WLG) recipients at a single center in Latin America. METHODS: A single-center retrospective review performed between August 2009 and December 2015...
November 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/29149968/effects-of-renal-transplantation-on-female-sexual-dysfunction-comparative-study-with-hemodialysis-and-a-control-group
#15
F O Kurtulus, M Y Salman, A Fazlioglu, B Fazlioglu
OBJECTIVES: Sexual dysfunction occurs commonly in individuals with end-stage renal disease. Chronic renal failure as well as the treatments used for it generally has a negative impact on sexual function with a subsequent increase in the risk of depression. There is scarcity of published data on female sexual dysfunction and the degree of improvement in patients on hemodialysis (HD) and transplant (Tx) recipients. The aim of this study was to compare the sexual function and degree of depression in HD and Tx patients with control group...
November 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/29149905/the-kusamala-program-for-primary-caregivers-of-children-6-59-months-of-age-hospitalized-with-severe-acute-malnutrition-in-malawi-study-protocol-for-a-cluster-randomized-controlled-trial
#16
Allison I Daniel, Meta van den Heuvel, Wieger P Voskuijl, Melissa Gladstone, Mike Bwanali, Isabel Potani, Celine Bourdon, Jenala Njirammadzi, Robert H J Bandsma
BACKGROUND: Severe acute malnutrition (SAM) is associated with high mortality rates and impairments in growth and development in children that do survive. There are complex nutritional, health, and behavioural risk factors involving severely malnourished children and their primary caregivers, requiring integrated intervention approaches. METHODS: A cluster-randomized controlled trial at the Queen Elizabeth Central Hospital in Blantyre, Malawi will be conducted to evaluate the effectiveness of a 4-day hospital-based intervention programme directed at primary caregivers...
November 17, 2017: Trials
https://www.readbyqxmd.com/read/29149901/development-and-exploration-of-the-content-validity-of-a-patient-reported-outcome-measure-to-evaluate-the-impact-of-migraine-the-migraine-physical-function-impact-diary-mpfid
#17
Asha Hareendran, Sally Mannix, Anne Skalicky, Martha Bayliss, Andrew Blumenfeld, Dawn C Buse, Pooja R Desai, Brian G Ortmeier, Sandhya Sapra
BACKGROUND: Adults with migraine experience substantial reductions in quality of life during and in-between migraine attacks. Clinical and regulatory guidelines encourage the inclusion of patient reported outcomes for the evaluation of benefits of interventions for migraine. METHODS: The conceptual framework and items for a new patient-reported outcome (PRO) instrument, the Migraine Physical Function Impact Diary (MPFID), were developed using scientific methods recommended to ensure content validity of PRO instruments...
November 17, 2017: Health and Quality of Life Outcomes
https://www.readbyqxmd.com/read/29149842/italian-version-of-nursing-students-perception-of-instructor-caring-i-nspic-assessment-of-reliability-and-validity
#18
C Arrigoni, M Puci, A M Grugnetti, L Collivasone, E Fenizia, P Borrelli, E Vellone, R Alvaro, M Piredda, M G De Marinis
BACKGROUND: Clinical experience is an essential component of nursing education since it provides students with the opportunity to construct and develop clinical competencies. Instructor caring is a pivotal facilitator at the forefront of clinical education, playing a key and complex educating role in clinical sectors. For these reasons the aims of this study was to assess the validity and reliability of the Italian version of NSPIC (I-NSPIC). METHODS: A validation multicentre study was conducted in three different Italian universities...
November 17, 2017: BMC Medical Education
https://www.readbyqxmd.com/read/29149813/structural-analysis-of-cd59-of-chinese-tree-shrew-a-new-reference-molecule-for-human-immune-system-specific-cd59-drug-discovery
#19
Subhamay Panda, Leena Kumari, Santamay Panda
BACKGROUND: Chinese tree shrews (Tupaia belangeri chinensis) bear several characteristics that are considered to be very crucial for utilizing in animal experimental models in biomedical research. Subsequent to the identification of key aspects and signaling pathways in nervous and immune systems, it is revealed that tree shrews acquires shared common as well as unique characteristics, and hence offers a genetic basis for employing this animal as a prospective model for biomedical research...
November 17, 2017: Current Drug Discovery Technologies
https://www.readbyqxmd.com/read/29149790/impressions-of-using-the-cohen-mansfield-agitation-inventory-as-an-outcome-measure-lessons-learnt-for-future-clinical-researchers-innovative-practice
#20
Lindsey Brett, Victoria Traynor, Shahla Meedya, Paul Stapley
The Cohen-Mansfield Agitation Inventory was a tool originally developed for use in research to measure agitation and subsequently used in clinical settings. It was the primary outcome measure for a randomised controlled trial which evaluated the effects and feasibility of a physiotherapist-led physical exercise intervention on agitation and physical performance of individuals living with dementia in nursing homes. The study produced weak results in regards to the Cohen-Mansfield Agitation Inventory due to small sample size and perceived issues with the use of the Cohen-Mansfield Agitation Inventory...
January 1, 2017: Dementia
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