keyword
https://read.qxmd.com/read/38262257/comparative-molecular-epidemiology-subtype-distribution-and-zoonotic-potential-of-blastocystis-sp-in-equus-animals-horses-donkeys-and-mules-in-northwestern-iran
#21
JOURNAL ARTICLE
Ali Asghari, Amirhosein Yousefi, Mohammad Reza Mohammadi, Roya Badali, Laya Shamsi, Ahmet Efe Köseoğlu, Amir Abbaszadeh, Morteza Shams, Behnam Mohammadi-Ghalehbin
A total of 500 fecal samples were collected from Equus animals in six different cities (Ardabil, Namin, Nir, Meshginshahr, Germi, and Khalkhal) of Ardabil Province, northwestern Iran, with 200 samples from horses, 200 from donkeys, and 100 from mules. Of the horse samples, 100 were from racing horses under special monitoring and care, while the remaining 100 were from non-racing horses, including those used for herding or in rural areas. All fecal samples were examined for the presence of Blastocystis sp. using PCR amplification of the SSU rRNA gene's barcode region after DNA extraction...
January 6, 2024: Comparative Immunology, Microbiology and Infectious Diseases
https://read.qxmd.com/read/38257996/an-analysis-of-the-gut-microbiota-and-related-metabolites-following-pcsk9-inhibition-in-statin-treated-patients-with-elevated-levels-of-lipoprotein-a
#22
JOURNAL ARTICLE
Jose A Caparrós-Martín, Patrice Maher, Natalie C Ward, Montserrat Saladié, Patricia Agudelo-Romero, Stephen M Stick, Dick C Chan, Gerald F Watts, Fergal O'Gara
BACKGROUND: Atherosclerotic cardiovascular disease (ASCVD) is a leading cause of global mortality, often associated with high blood levels of LDL cholesterol (LDL-c). Medications like statins and PCSK9 inhibitors, are used to manage LDL-c levels and reduce ASCVD risk. Recent findings connect the gut microbiota and its metabolites to ASCVD development. We showed that statins modulate the gut microbiota including the production of microbial metabolites involved in the regulation of cholesterol metabolism such as short chain fatty acids (SCFAs) and bile acids (BAs)...
January 15, 2024: Microorganisms
https://read.qxmd.com/read/38257988/comparison-of-experimental-methodologies-based-on-bulk-metagenome-and-virus-like-particle-enrichment-pros-and-cons-for-representativeness-and-reproducibility-in-the-study-of-the-fecal-human-virome
#23
JOURNAL ARTICLE
Adriana Soria-Villalba, Nicole Pesantes, Nuria Jiménez-Hernández, Javier Pons, Andrés Moya, Vicente Pérez-Brocal
Studies on the human virome based on the application of metagenomic approaches involve overcoming a series of challenges and limitations inherent not only to the biological features of viruses, but also to methodological pitfalls which different approaches have tried to minimize. These approaches fall into two main categories: bulk-metagenomes and virus-like particle (VLP) enrichment. In order to address issues associated with commonly used experimental procedures to assess the degree of reliability, representativeness, and reproducibility, we designed a comparative analysis applied to three experimental protocols, one based on bulk-metagenomes and two based on VLP enrichment...
January 13, 2024: Microorganisms
https://read.qxmd.com/read/38257911/impact-of-dietary-regime-and-seasonality-on-hindgut-s-mycobiota-diversity-in-dairy-cows
#24
JOURNAL ARTICLE
Ali Sadek, Bernard Taminiau, Georges Daube, Panagiotis Sapountzis, Frédérique Chaucheyras-Durand, Mathieu Castex, Françoise Coucheney, Djamel Drider
We describe and discuss the intestinal mycobiota of dairy cows reared in France following variations in dietary regimes and two seasons. Two groups of 21 animals were followed over a summer and winter period, and another group of 28 animals was followed only during the same summer season. The summer diet was based on grazing supplemented with 3-5 kg/d of maize, grass silage and hay, while the winter diet consisted of 30% maize silage, 25% grass silage, 15% hay and 30% concentrate. A total of 69 DNA samples were extracted from the feces of these cows...
December 31, 2023: Microorganisms
https://read.qxmd.com/read/38257139/circadian-rhythm-perturbation-aggravates-gut-microbiota-dysbiosis-in-dextran-sulfate-sodium-induced-colitis-in-mice
#25
JOURNAL ARTICLE
Joseph Amara, Tarek Itani, Joelle Hajal, Jules-Joel Bakhos, Youakim Saliba, Saied A Aboushanab, Elena G Kovaleva, Nassim Fares, Alicia C Mondragon, Jose Manuel Miranda
Circadian rhythm disruption is increasingly considered an environmental risk factor for the development and exacerbation of inflammatory bowel disease. We have reported in a previous study that nychthemeral dysregulation is associated with an increase in intestinal barrier permeability and inflammation in mice with dextran sulfate sodium (DSS)-induced colitis. To investigate the effect of circadian rhythm disruption on the composition and diversity of the gut microbiota (GM), sixty male C57BL/6J mice were initially divided to two groups, with the shifted group ( n = 30) exposed to circadian shifts for three months and the non-shifted group ( n = 30) kept under a normal light-dark cycle...
January 12, 2024: Nutrients
https://read.qxmd.com/read/38250201/outbreak-of-diarrhea-caused-by-a-novel-cryptosporidium-hominis-subtype-during-british-military-training-in-kenya
#26
JOURNAL ARTICLE
Romeo Toriro, Scott Pallett, Stephen Woolley, Charlie Bennett, Isra Hale, Jennifer Heylings, Daniel Wilkins, Thomas Connelly, Kennedy Muia, Patrick Avery, Andrew Stuart, Laura Morgan, Mark Davies, William Nevin, Oliver Quantick, Guy Robinson, Kristin Elwin, Rachel Chalmers, Daniel Burns, Nicholas Beeching, Thomas Fletcher, Matthew O'Shea
BACKGROUND: We report clinical, epidemiological, and laboratory features of a large diarrhea outbreak caused by a novel Cryptosporidium hominis subtype during British military training in Kenya between February and April 2022. METHODS: Data were collated from diarrhea cases, and fecal samples were analyzed on site using the multiplex polymerase chain reaction (PCR) BioFire FilmArray. Water was tested using Colilert kits (IDEXX, UK). DNA was extracted from feces for molecular characterization of Cryptosporidium A135 , Lib13 , ssu rRNA , and gp60 genes...
January 2024: Open Forum Infectious Diseases
https://read.qxmd.com/read/38227220/phylogenetic-analysis-of-eimeria-tenella-isolates-from-chicken-of-sub-tropical-mountains-of-meghalaya-india
#27
JOURNAL ARTICLE
Meena Das, Nampher Masharing, Mun Mun Makri
BACKGROUND: Coccidiosis is the most common and pathogenic intestinal disease caused by different species of Eimeria is chicken. In this study, we describe the prevalence, molecular diagnosis and evolutionary insight of Eimeria tenella in chicken of Meghalaya's sub-tropical mountainous area. METHODS AND RESULTS: Faecal samples (337 no.) and dead chicks (298 no.) were collected every month from January to July' 2023 from poultry farms (4nos.) in and around Umiam, Ri-Bhoi, Meghalaya...
January 16, 2024: Molecular Biology Reports
https://read.qxmd.com/read/38222790/comparative-study-of-type-2-diabetes-mellitus-associated-gut-microbiota-between-the-dai-and-han-populations
#28
JOURNAL ARTICLE
Ling-Tong Tang, Lei Feng, Hui-Ying Cao, Rui Shi, Bei-Bei Luo, Yan-Bi Zhang, Yan-Mei Liu, Jian Zhang, Shuang-Yue Li
BACKGROUND: The global prevalence of type 2 diabetes mellitus (T2DM) is increasing. T2DM is associated with alterations of the gut microbiota, which can be affected by age, illness, and genetics. Previous studies revealed that there are discriminating microbiota compositions between the Dai and the Han populations. However, the specific gut microbiota differences between the two populations have not been elucidated. AIM: To compare the gut microbiota differences in subjects with and without T2DM in the Dai and Han populations...
December 15, 2023: World Journal of Diabetes
https://read.qxmd.com/read/38152257/fecal-prevalence-of-campylobacter-spp-in-house-dogs-in-lebanon-a-pilot-study
#29
JOURNAL ARTICLE
Ghassan Ghssein, Rana Barakat, Nada Nehme, Rana Awada, Hussein F Hassan
BACKGROUND AND AIM: Campylobacter spp. are Gram-negative bacilli that are widely recognized as a primary cause of bacterial gastroenteritis worldwide. Campylobacteriosis is the disease caused by this pathogen. Recently, greater attention has been given to the prevalence of campylobacteriosis in different animals, including pets. These animals are considered to be significant reservoirs for this zoonosis. In Lebanon, the occurrence of Campylobacter infection is high. Our first-of-its-kind pilot study in Lebanon aimed to estimate the fecal prevalence of Campylobacter species in house dogs...
November 2023: Veterinary World
https://read.qxmd.com/read/38140286/comparative-analysis-of-gut-microbiota-from-rats-induced-by-se-deficiency-and-t-2-toxin
#30
JOURNAL ARTICLE
Yifan Wu, Yi Gong, Yu Zhang, Shujin Li, Chaowei Wang, Yuequan Yuan, Xi Lv, Yanli Liu, Feihong Chen, Sijie Chen, Feiyu Zhang, Xiong Guo, Xi Wang, Yujie Ning, Hongmou Zhao
The aim of this study was to analyze the differences in gut microbiota between selenium deficiency and T-2 toxin intervention rats. Knee joint and fecal samples of rats were collected. The pathological characteristics of knee cartilage were observed by safranin O/fast green staining. DNA was extracted from fecal samples for PCR amplification, and 16S rDNA sequencing was performed to compare the gut microbiota of rats. At the phylum level, Firmicutes (81.39% vs. 77.06%) and Bacteroidetes (11.11% vs. 14.85%) were dominant in the Se-deficient (SD) group and T-2 exposure (T-2) groups...
December 7, 2023: Nutrients
https://read.qxmd.com/read/38114587/multicenter-evaluation-of-gut-microbiome-profiling-by-next-generation-sequencing-reveals-major-biases-in-partial-length-metabarcoding-approach
#31
JOURNAL ARTICLE
Hugo Roume, Stanislas Mondot, Adrien Saliou, Sophie Le Fresne-Languille, Joël Doré
Next-generation sequencing workflows, using either metabarcoding or metagenomic approaches, have massively contributed to expanding knowledge of the human gut microbiota, but methodological bias compromises reproducibility across studies. Where these biases have been quantified within several comparative analyses on their own, none have measured inter-laboratory reproducibility using similar DNA material. Here, we designed a multicenter study involving seven participating laboratories dedicated to partial- (P1 to P5), full-length (P6) metabarcoding, or metagenomic profiling (MGP) using DNA from a mock microbial community or extracted from 10 fecal samples collected at two time points from five donors...
December 18, 2023: Scientific Reports
https://read.qxmd.com/read/38110960/prevalence-and-first-molecular-identification-of-sarcocystis-species-in-feces-of-domestic-dogs-canis-familiaris-in-egypt
#32
JOURNAL ARTICLE
Ismail S Elshahawy, Marwa Fawaz, Aya Gomaa, Eman Mohammed
BACKGROUND: Sarcocystis species are obligatorily heteroxenous protozoan parasites with predator-prey life cycles. Global Knowledge about the epidemiology and the distribution pattern of different Sarcocystis species in dog feces are very scarce. Therefore, the current investigation was conducted to declare the occurrence of Sarcocystis in the fecal specimens of the most common canids in Egypt, the domestic dogs, and to identify the species present using various parasitological and molecular approaches...
December 18, 2023: BMC Veterinary Research
https://read.qxmd.com/read/38105316/the-gut-microbiota-composition-and-metabolites-are-different-in-women-with-hypertensive-disorders-of-pregnancy-and-normotension-a-pilot-study
#33
JOURNAL ARTICLE
Zhouyi Wu, Mengdi Ge, Jinsu Liu, Xiaoqing Chen, Zhiqiang Cai, Huan Huang
INTRODUCTION: Hypertensive disorders of pregnancy (HDP) are one of the main causes of perinatal morbidity. Gut microbiota influences host inflammatory pathways, glucose, and lipid metabolism. However, there is a lack of studies available on gut microbiota in HDP. OBJECTIVES: We investigate the mechanistic and pathogenic role of microbiota in the development of HDP, and want to treat HDP with gut microbiota. METHODS: We performed a case-control study to compare fecal samples of HDP and normotensive pregnant women by 16S ribosomal RNA sequencing...
December 17, 2023: Journal of Obstetrics and Gynaecology Research
https://read.qxmd.com/read/38086952/assessment-trial-of-the-effect-of-enteral-insulin-on-the-preterm-infant-intestinal-microbiota
#34
JOURNAL ARTICLE
Bárbara Moreno-Sanz, Fernando Lázaro-Perona, Esperanza Escribano, Marta Cabrera Lafuente, María Teresa Montes, Rocío Amorós, Silvia Arboleya, Miguel Gueimonde, Jesús Mingorance, Miguel Saenz de Pipaon
BACKGROUND: Insulin might be associated with changes in infant gastrointestinal microbiota. The objective of this randomized controlled trial was to assess the efficacy of two doses of recombinant human(rh) enteral insulin administration compared to placebo in intestinal microbiota. METHODS: 19 preterm patients were recruited at the NICU of La Paz University Hospital (Madrid, Spain). Subjects received 2000 µIU of rh enteral insulin/ml(n = 8), 400 µIU of rh enteral insulin/ml(n = 6) or placebo(n = 5) for 28 days administered once per day...
December 12, 2023: Pediatric Research
https://read.qxmd.com/read/38055436/identification-of-eimeria-spp-in-domestic-chickens-raised-in-alternative-poultry-production-systems-in-the-state-of-s%C3%A3-o-paulo-brazil
#35
JOURNAL ARTICLE
José Carlos Soares Júnior, Bruno Ferraz Itoyama, Bruna Matarucco Sampaio Beretta, Camila Michele de Souza Hossotani, Maria Santa Cardoso Silva, Giane Serafim da Silva, Alex Akira Nakamura, Flávia Lombardi Lopes, Marcelo Vasconcelos Meireles
The objective of this study was to identify Eimeria spp. in alternative poultry production systems (APPS) in the State of São Paulo, Brazil. Fecal samples (168) and DNA extracted from fecal samples obtained in APPS located in different Municipalities in the State of São Paulo (93) were examined by microscopy or genera-specific PCR (ITS-1 locus). Samples positive for Eimeria spp. were examined using Eimeria lata, Eimeria nagambie, and Eimeria zaria species-specific PCR protocols (ITS-2 locus) and another E...
2023: Brazilian Journal of Veterinary Parasitology
https://read.qxmd.com/read/38043772/metagenomic-analysis-of-microbial-communities-and-antibiotic-resistant-genes-in-the-tijuana-river-and-potential-sources
#36
JOURNAL ARTICLE
Shayla Shahar, Karilyn E Sant, Nicholas Allsing, Scott T Kelley
The Tijuana River is a transborder river that flows northwest across the border from Baja California in Mexico into Southern California before discharging into the Pacific Ocean. The river is frequently contaminated with raw sewage due to inadequate sanitary infrastructure in Tijuana. To assess the type and degree of microbial contamination, water samples were collected monthly from a near-border and an estuarine site from August 2020 until May 2021. A portion of each sample was used for epifluorescent microscopy and DNA was extracted directly from the rest for shotgun metagenomic sequencing...
December 1, 2023: Environmental Pollution
https://read.qxmd.com/read/38038385/characterization-of-markers-functional-properties-and-microbiome-composition-in-human-gut-derived-bacterial-extracellular-vesicles
#37
JOURNAL ARTICLE
Chih-Chi Li, Wei-Fan Hsu, Po-Chieh Chiang, Ming-Che Kuo, Andrew M Wo, Yufeng Jane Tseng
Past studies have confirmed the etiologies of bacterial extracellular vesicles (BEVs) in various diseases, including inflammatory bowel disease (IBD) and colorectal cancer (CRC). This study aimed to investigate the characteristics of stool-derived bacterial extracellular vesicles (stBEVs) and discuss their association with stool bacteria. First, three culture models - gram-positive (G+) Bc BEVs (from B.coagulans ), gram-negative (G-) Ec BEVs (from E.coli ), and eukaryotic cell-derived EVs (EEV, from Colo205 cell line) - were used to benchmark various fractions of stEVs separated from optimized density gradient approach (DG)...
December 2023: Gut Microbes
https://read.qxmd.com/read/38030815/a-novel-bifidobacterium-klebsiella-ratio-in-characterization-analysis-of-the-gut-and-bile-microbiota-of-cca-patients
#38
JOURNAL ARTICLE
Ningning Zhang, Wenwen Zhu, Shuwen Zhang, Tian Liu, Lan Gong, Zeyu Wang, Wei Zhang, Yunlong Cui, Qiang Wu, Jingtong Li, Hao Yu, Emad M El-Omar, Jihui Hao, Wei Lu
Cholangiocarcinoma (CCA) is a serious health problem worldwide. The gut and bile microbiota have not been clearly characterized in patients with CCA, and better noninvasive diagnostic approaches for CCA need to be established. The aim of this study was to investigate the characteristics of the gut and bile microbiota in CCA patients. Forty-two CCA patients and 16 healthy normal controls (HNCs) were enrolled. DNA was extracted from fecal and bile samples and subjected to 16S rRNA gene analysis. We found that there were significant differences in the species diversity, structure, and composition of the microbial communities between the CCA group and the HNC grouAt the phylum level, compared with that in the HNC group, the relative abundance of Firmicutes and Actinobacteriota was significantly decreased in the CCA group, whereas Proteobacteria and Bacteroidota were significantly enriched...
November 30, 2023: Microbial Ecology
https://read.qxmd.com/read/38022552/dysbiosis-of-the-gut-microbiota-as-a-susceptibility-factor-for-kawasaki-disease
#39
JOURNAL ARTICLE
Yoshiki Teramoto, Shohei Akagawa, Shin-Ichiro Hori, Shoji Tsuji, Koichiro Higasa, Kazunari Kaneko
INTRODUCTION: Gut microbial imbalance (dysbiosis) has been reported in patients with acute Kawasaki disease (KD). However, no studies have analyzed the gut microbiota while focusing on susceptibility to KD. This study aimed to evaluate whether dysbiosis elevates susceptibility to KD by assessing children with a history of KD. METHODS: Fecal DNA was extracted from 26 children with a history of KD approximately 1 year prior (KD group, 12 boys; median age, 32.5 months; median time from onset, 11...
2023: Frontiers in Immunology
https://read.qxmd.com/read/38004740/two-dose-ceftiofur-treatment-increases-cephamycinase-gene-quantities-and-fecal-microbiome-diversity-in-dairy-cows-diagnosed-with-metritis
#40
JOURNAL ARTICLE
Claudia Ossa-Trujillo, Ethan A Taylor, Fatima Sarwar, Javier Vinasco, Ellen R Jordan, Jose A García Buitrago, G Robert Hagevoort, Sara D Lawhon, Juan M Piñeiro, Jessica Galloway-Peña, Keri N Norman, Harvey Morgan Scott
Antimicrobial resistance is a significant concern worldwide; meanwhile, the impact of 3rd generation cephalosporin (3GC) antibiotics on the microbial communities of cattle and resistance within these communities is largely unknown. The objectives of this study were to determine the effects of two-dose ceftiofur crystalline-free acid (2-CCFA) treatment on the fecal microbiota and on the quantities of second-and third-generation cephalosporin, fluoroquinolone, and macrolide resistance genes in Holstein-Friesian dairy cows in the southwestern United States...
November 8, 2023: Microorganisms
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