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amniotic fluid

Wansun Kim, Soo Hyun Lee, Jin Hwi Kim, Yong Jin Ahn, Yeon-Hee Kim, Jae Su Yu, Samjin Choi
We report the development of a surface-enhanced Raman spectroscopy (SERS) sensor chip by decorating gold nanoparticles (AuNPs) on ZnO nanorod (ZnO NR) arrays vertically grown on cellulose paper (C). We show that these chips can enhance the Raman signal by 1.25×107 with an excellent reproducibility of <6%. We show that we can measure trace amounts of human amniotic fluids of patients with subclinical intra-amniotic infection (IAI) and preterm delivery (PTD) using the chip in combination with a multivariate statistics-derived machine learning-trained bioclassification method We can detect the presence of prenatal diseases and identify the types of diseases from amniotic fluids with >92% clinical sensitivity and specificity...
June 19, 2018: ACS Nano
Mario Barilani, Federica Banfi, Silvia Sironi, Enrico Ragni, Salomé Guillaumin, Francesca Polveraccio, Lorenzo Rosso, Monica Moro, Giuseppe Astori, Michela Pozzobon, Lorenza Lazzari
Human multipotent mesenchymal stromal cells (MSC) are isolated from a plethora of tissue sources for cell therapy purposes. In 2006, the International Society for Cellular Therapy (ISCT) published minimal guidelines to define MSC identity. Nevertheless, many independent studies demonstrated that cells meeting the ISCT criteria possessed heterogeneous phenotypes and functionalities, heavily influenced by culture conditions. In this study, human MSC derived from many adult (bone marrow and adipose tissue) or fetal (cord blood, Wharton's jelly, umbilical cord perivascular compartment and amniotic fluid) tissues were investigated...
June 18, 2018: Scientific Reports
Priyanka Jha, Spencer Behr, Tara Morgan, Erin Washburn, Jennifer Lucero, Lee-May Chen, Liina Poder
Placental abruption is an important cause of feto-maternal hemorrhage, with significant impact on both fetal and maternal mortality. In most cases, it presents with abdominal pain and vaginal bleeding. However, vaginal bleeding may be absent with concealed intra-amniotic hemorrhage, as in cases with placenta previa, hence confounding this diagnosis. In such cases, imaging studies may be obtained to evaluate for abdominal pain in pregnancy; hence, radiologists should be aware of the ultrasound and magnetic resonance (MR) imaging appearance of intra-amniotic hemorrhage...
June 17, 2018: Emergency Radiology
DengLiang Wang, Quan Kang, ShuJun Shi, Wei Hu
To summarize the clinical characteristics, diagnosis, treatment and prognosis among 152 children with annular pancreas (AP). A retrospective review of 152 patients with AP who were treated with surgical repair between January 2009 and August 2017 was performed at our pediatric surgical units. Presenting symptoms, birth weight, radiological findings, associated anomalies, the type of surgery performed were analyzed. (1) 152 patients were identified, out of which 82 were males, and 70 were females; (2) the average birth weight of children with AP was less than that of healthy newborns...
June 16, 2018: Pediatric Surgery International
Vanesa Rodríguez Fernández, Carlos Nicolás López Ramón Y Cajal, Elena Marín Ortiz, Emilio Couceiro Naveira
OBJECTIVE: To determine the intrapartum and perinatal results associated with different degrees of staining of meconium stained amniotic fluid (MSAF). STUDY DESIGN: In a retrospective cohort study of all singleton deliveries over a period of one year (2015) in a tertiary hospital, we compared different degrees of MSAF (yellow, green and thick) to clear amniotic fluids, and analysed in each group maternal, intrapartum and neonatal variables as well as umbilical cord blood gas analysis...
March 20, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Jun Hu, Hua Wang, Yong-Fang Hu, Xiao-Feng Xu, Yuan-Hua Chen, Mi-Zhen Xia, Cheng Zhang, De-Xiang Xu
INTRODUCTION: Several reports demonstrated that cadmium (Cd) had proinflammatory activities. The present study aimed to investigate whether Cd induces inflammatory cytokines in mouse placenta and human trophoblast cells. METHODS: Human JEG-3 cells were treated with different concentration of CdCl2 (0-50 μM) or CdCl2 (25 μM) for different times. The pregnant mice were administered with CdCl2 (3.0 mg/kg, i.p.) on GD15. RESULTS: TNF-α, IL-8 and IL-6 mRNAs were elevated in CdCl2 -treated JEG-3 cells...
May 2018: Placenta
Claudia Patricia Cataño Sabogal, Javier Fonseca, Herney Andrés García-Perdomo
INTRODUCTION: The aim of this study was to determine the diagnostic performance of different diagnostic tests for histologic chorioamnionitis in patients at more than 20 weeks of gestation. METHODS: A systematic search was carried out through MEDLINE, EMBASE, LILACS, CENTRAL and unpublished literature. Observational studies included with pregnant women (>20 weeks) with chorioamnionitis. The reference standard was the histopathological study of the placenta, umbilical cord and fetal membranes...
June 9, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Nicola Alessio, Caterina Pipino, Domitilla Mandatori, Pamela Di Tomo, Angela Ferone, Marco Marchiso, Mariarosa A B Melone, Gianfranco Peluso, Assunta Pandolfi, Umberto Galderisi
Mesenchymal stromal cells (MSCs) are considered to be an excellent source in regenerative medicine. They contain several cell subtypes, including multipotent stem cells. MSCs are of particular interest as they are currently being tested using cell and gene therapies for a number of human diseases. They represent a rare population in tissues; for this reason, they require, before being transplanted, an in vitro amplification. This process may induce replicative senescence, thus affecting differentiation and proliferative capacities...
June 15, 2018: Journal of Cellular Physiology
Alexis C Thompson, Casey Feeney, Mark B Kristal
Placental Opioid Enhancing Factor (POEF) is found in amniotic fluid (AF) and placenta. When ingested, it enhances opioid-mediated pain relief. Our laboratory has shown that ingestion of AF specifically enhances the hypoalgesia associated with δ-opioid receptor activation in the brain. The specific biochemical compound in AF responsible for the enhancement of δ-opioid activity is of great interest as an analgesic adjunct for pain but is unknown at this time. Research efforts to isolate and characterize this biochemical compound are hampered by the lack of an algesiometric assay that allows repeated measurement of pain threshold and repeated exposure to δ-opioid receptor activation...
June 11, 2018: Brain Research
Massimiliano Perduca, Stefania Nicolis, Barbara Mannucci, Monica Galliano, Hugo L Monaco
Retinol is transported in vertebrate plasma bound to a protein called retinol-binding protein (RBP4) so far believed to be specific for the vitamin. When the protein is saturated with retinol it binds tightly to another plasma protein, transthyretin while when not saturated with retinol it does not bind to TTR (Goodman, 1984). The X-ray structures of human RBP4, holo and devoid of retinol in its binding site are known to resolutions of 2.0 and 2.5 Å (Cowan et al., 1990; Zanotti et al., 1993) [2], [3]. We have shown that RBP4 is not specific for retinol but it is also found in plasma, urine and amniotic fluid bound to fatty acids...
June 2018: Data in Brief
Peixuan Cao, Xiangyu Zhu, Ying Yang, Yujie Zhu, Jie Li
OBJECTIVE: To apply high-throughput sequencing for the detection of potential mutation in a methylmalonic academia pedigree for which no proband was available. METHODS: For a couple who had previously given birth to an affected child, 14 genes were re-sequenced by high-throughput sequencing. Suspected mutations were validated by Sanger sequencing. Specific mutations were tested for amniotic fluid sample from the fetus. RESULTS: High-throughput sequencing suggested that the husband has carried a heterozygous mutation of the MUT gene (Exon 3: c...
June 10, 2018: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
Kari A Teramo, Miira M Klemetti, John A Widness
Fetal erythropoietin (EPO), in addition to regulating erythropoiesis, has also tissue-protective properties based on its anti-inflammatory, anti-apoptotic, antioxidant, and neurotrophic effects. Notably, EPO concentrations needed for tissue protection are 100-1000 times higher than concentrations needed for regulating erythropoiesis. This dual effect of EPO is based on EPO-receptor (EPO-R) isoforms, which differ structurally and functionally. We hypothesize in this Integrated Mechanism Review that during severe fetal hypoxia the observed, but poorly understood, marked increases of fetal plasma EPO concentrations occur to protect the brain, heart, and other vital fetal organs...
June 12, 2018: Pediatric Research
Hiroyuki Tsuda, Tomomi Kotani, Tomoko Nakano, Kenji Imai, Takafumi Ushida, Akihiro Hirakawa, Fumie Kinoshita, Yuichiro Takahashi, Shigenori Iwagaki, Fumitaka Kikkawa
BACKGROUND: Whether or not the period of fetal lung maturity differs between twin and singleton pregnancies has not been clarified. We examined whether or not fetal lung maturity and fetal lung absorption are achieved earlier in twin fetuses than in singleton fetuses. METHODS: We registered 454 singleton pregnancies and 398 twin pregnancies with no congenital abnormalities affecting the respiratory function or neonatal deaths. All patients were delivered by Caesarean section without labor between 24 and 38 gestational weeks...
June 9, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Ivana Musilova, Ctirad Andrys, Helena Hornychova, Lenka Pliskova, Marcela Drahosova, Barbora Zednikova, Radka Bolehovska, Tomas Faist, Bo Jacobsson, Marian Kacerovsky
OBJECTIVE: To determine changes in the intraamniotic environment during the latency period using paired amniotic and gastric fluid samples in pregnancies complicated by preterm prelabor rupture of membranes (PPROM). METHODS: A total of 34 women with singleton pregnancies complicated by PPROM prior to 34 weeks were included in the study. Amniotic fluid was obtained by transabdominal amniocentesis at the time of admission. Immediately after delivery, umbilical cord blood and gastric fluid were obtained...
May 31, 2018: Pediatric Research
Shilpi Chabra
BACKGROUND: The approach to intrapartum and postnatal management of an infant born through meconium-stained amniotic fluid (MSAF) in the delivery room (DR) has changed several times over the last few decades, leading to confusion and anxiety among health care providers (nurses, nurse practitioners, respiratory therapists, midwives, and physicians). This article provides state-of-the-art insight into the evidence or lack thereof for the changes in guidelines. PURPOSE: To discuss the evidence for evolution of DR management of vigorous and nonvigorous infants born through any type of MSAF...
June 8, 2018: Advances in Neonatal Care: Official Journal of the National Association of Neonatal Nurses
Y Liu, Y P Liu, Y Zhang, J Q Song, H Zheng, H Dong, Y Y Ma, T F Wu, Q Wang, X Y Li, Y Ding, D X Li, Y Jin, M Q Li, Z X Wang, Y Yuan, H X Li, J Qin, Y L Yang
Objective: To analyz the current situation of the diagnosis, treatment and prevention of methylmalonic acidemia, the phenotypes, biochemical features and genotypes of the patients in the mainland of China, were investigated. Methods: Tottally 1 003 patients of methylmalonic acidemia from 26 provinces and municipalities of the mainland of China were enrolled. The clinical data, biochemical features and gene mutations were studied. Blood aminoacids and acylcarnitines, urine organic acids, and plasma total homocysteine were determined for the biochemical diagnosis...
June 2, 2018: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
Rita Romani, Giorgia Manni, Chiara Donati, Irene Pirisinu, Caterina Bernacchioni, Marco Gargaro, Matteo Pirro, Mario Calvitti, Giorgia Manni, Francesco Bagaglia, Amirhossein Sahebkar, Graziano Clerici, Davide Matino, Giovanni Pomili, Gian Carlo Di Renzo, Vincenzo Nicola Talesa, Paolo Puccetti, Francesca Fallarino
Stem cells have high potential for cell therapy in regenerative medicine. We previously isolated stem cell types from human amniotic fluid, derived from prenatal amniocentesis. One type, characterized by a fast doubling time, was designated as fast human amniotic stem cells (fHASCs). These cells exhibited high differentiation potential and immunoregulatory properties. Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that influences stem-cell pluripotency, differentiation, mobility, and regulates immune functions...
June 7, 2018: European Journal of Pharmacology
Jade Gieseke Guevara, Swati Agarwal-Sinha
BACKGROUND: As the number of children with Zika virus-related complications grows, the long-term developmental trajectory and its effects on families are unknown. We present the first known case of congenital Zika syndrome seen at our institution with significant fundus findings. CASE PRESENTATION: A 3-day-old Hispanic baby girl presented with severe microcephaly of 24 cm and temperature instability at birth. Her mother had traveled to Honduras early in pregnancy and testing of amniotic fluid was positive for Zika virus via polymerase chain reaction...
June 9, 2018: Journal of Medical Case Reports
Emre Zafer, Cigdem Yenisey, Meryem Kurek Eken, Ezgi Ozdemir, Imran Kurt Omurlu, Hasan Yuksel
The aim of the present study was to explore the possible relations of maternal serum and amniotic fluid nitric oxide (NO) and of vascular endothelial growth factor A (VEGF-A) concentrations with uterine artery Doppler indices (UtADs) in a healthy second trimester obstetric population. In this regard, the levels of NO and VEGF-A were measured in maternal sera and the amniotic fluid samples were in 36 subjects who were in their early second trimester of pregnancy. The mean levels for VEGF-A were 110.3 ± 31...
June 8, 2018: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
Stella Liong, Ratana Lim, Caitlyn Nguyen-Ngo, Gillian Barker, Helena C Parkington, Martha Lappas
There is currently no effective treatments to prevent spontaneous preterm labour. The precise upstream biochemical pathways that regulate the transition between uterine quiescence during pregnancy and contractility during labour remain unclear. It is well known however that intrauterine inflammation, including infection, is commonly associated with preterm labour. In this study, we identified the immunoproteasome subunit low-molecular-mass protein (LMP)7 mRNA expression to be significantly upregulated in labouring human myometrium...
June 8, 2018: European Journal of Immunology
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