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https://www.readbyqxmd.com/read/28104582/amniotic-fluid-derived-stem-cells-mixed-with-platelet-rich-plasma-for-restoration-of-rat-alveolar-bone-defect
#1
Minjiao Wang, Hongliang Li, Jiawen Si, Jiewen Dai, Jun Shi, Xudong Wang, Lihe Guo, Guofang Shen
Stem cells isolated from the amniotic fluid have been shown as a promising candidate for cell therapy and tissue engineering. However, the experimental and preclinical applications of amniotic fluid-derived stem cells (AFSCs) in the very field of maxillofacial bone tissue engineering are still limited. In this study, rat AFSCs were successfully harvested and characterized in vitro The rat AFSCs showed typical fibroblastoid morphology, stable proliferation activity and multi-differentiation potential. Flow-cytometry analysis demonstrated that these cells were positive for CD29, CD44, and CD90, while negative for hematopoietic markers such as CD34 and CD45...
January 18, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28103589/the-effects-of-body-mass-index-on-second-trimester-amniotic-fluid-cytokine-and-matrix-metalloproteinase-levels
#2
Rauf Melekoglu, Osman Ciftci, Sevil Eraslan, Nese Basak, Ebru Celik
AIM: The aim of this study was to determine the effects of obesity on amniotic fluid (AF) inflammatory markers in second-trimester AF, testing the hypothesis that there is a relationship between maternal body mass index (BMI) and fetal inflammatory exposure. METHODS: AF was obtained from 84 singleton pregnant women undergoing elective amniocentesis for karyotype analysis at 16-24 weeks of gestation between April 2014 and May 2016. The cell-free AF was used to analyze interleukin (IL)-1β and IL-6, and matrix metalloproteinase (MMP)-1, MMP-6, and MMP-13...
January 20, 2017: Gynecologic and Obstetric Investigation
https://www.readbyqxmd.com/read/28098911/diagnosis-for-choroideremia-in-a-large-chinese-pedigree-by-next%C3%A2-generation-sequencing-ngs-and-non%C3%A2-invasive-prenatal-testing-nipt
#3
Li Zhu, Jingliang Cheng, Boxu Zhou, Chunli Wei, Weichan Yang, Dong Jiang, Iqra Ijaz, Xiaojun Tan, Rui Chen, Junjiang Fu
To develop an effective strategy to isolate and use cell‑free fetal DNA (cffDNA) for the combined use of next‑generation sequencing (NGS) for diagnosing choroideremia and non‑invasive prenatal testing (NIPT) for Y chromosome determination, a large Chinese family with an X‑linked recessive disease, choroideremia, was recruited. Cell‑free DNA was extracted from maternal plasma, and SRY polymerase chain reaction amplification was performed using NIPT. Sanger sequencing was subsequently used for fetal amniotic fluid DNA verification...
January 13, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28098348/impact-of-lysosomal-storage-disorders-on-biology-of-mesenchymal-stem-cells-evidences-from-in-vitro-silencing-of-glucocerebrosidase-gba-and-alpha-galactosidase-a-gla-enzymes
#4
T Squillaro, I Antonucci, N Alessio, A Esposito, M Cipollaro, Mab Melone, G Peluso, L Stuppia, U Galderisi
Lysosomal storage disorders (LDS) comprise a group of rare multisystemic diseases resulting from inherited gene mutations that impair lysosomal homeostasis. The most common LSDs, Gaucher disease (GD) and Fabry disease (FD) are caused by deficiencies in the lysosomal glucocerebrosidase (GBA) and alpha-galactosidase A (GLA) enzymes, respectively. Given the systemic nature of enzyme deficiency, we hypothesized that the stem cell compartment of GD and FD patients might be also affected. Among stem cells, mesenchymal stem cells (MSCs) are a commonly investigated population given their role in hematopoiesis and the homeostatic maintenance of many organs and tissues...
January 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28094842/impact-of-microbial-invasion-of-amniotic-cavity-and-the-type-of-microorganisms-on-short-term-neonatal-outcome-in-women-with-preterm-labor-and-intact-membranes
#5
Teresa Cobo, Irene Vives, Adriano Rodríguez-Trujillo, Clara Murillo, Martina A Ángeles, Jordi Bosch, Andrea Vergara, Eduard Gratacós, Montse Palacio
INTRODUCTION: The objective was to evaluate the impact of microbial invasion of the amniotic cavity (MIAC) and the type of microorganisms on pregnancy and short-term neonatal outcomes in women with preterm labor (PTL). MATERIAL AND METHODS: Prospective observational cohort study including women with PTL from 22.0-36.0 weeks. MIAC was defined based on amniotic fluid aerobic/anaerobic/mycoplasmas cultures and intra-amniotic inflammation (IAI) based on amniotic fluid Interleukin-6 levels...
January 17, 2017: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/28077838/low-grade-metabolically-induced-inflammation-mediators-interleukin-6-adiponectin-and-tnf-%C3%AE-serum-levels-in-obese-pregnant-patients-in-the-perinatal-period
#6
Malgorzata Zembala-Szczerba, Andrzej Jaworowski, Hubert Huras, Dorota Babczyk, Robert Jach
BACKGROUND Obesity is a major clinical problem. The number of obese pregnant women is rising rapidly. The consequences of obesity are significant and affect every aspect of perinatal care for both the mother and the developing fetus. Adipose tissue may be responsible for chronic subclinical inflammation in obesity, being a source of inflammatory mediators. The study was designed to evaluate the analysis of the serum concentration of inflammatory mediators, including interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), and adiponectin, in obese pregnant women at full-term pregnancies...
January 12, 2017: Medical Science Monitor Basic Research
https://www.readbyqxmd.com/read/28077143/pregnant-women-carrying-microcephaly-foetuses-and-zika-virus-contain-potentially-pathogenic-microbes-and-parasites-in-their-amniotic-fluid
#7
Diogo Antonio Tschoeke, Louisi Souza de Oliveira, Luciana Leomil, Amilcar Tanuri, Fabiano Lopes Thompson
BACKGROUND: Microcephaly has become a major public health problem in Brazil. The total number of newborns with microcephaly was reported to be >4000 in June 2016. Studies suggest that Zika Virus is a major cause of new microcephaly cases in Brazil. Inside the uterus, the foetus is surrounded by the Amniotic Fluid, a proximal fluid that contains foetal and maternal cells as well as microorganisms and where Zika Virus was already found. CASE PRESENTATION: A previous study reported the presence of the Zika Virus in the amniotic fluid (collected in the 28th gestational week) of two pregnant women carrying microcephaly foetuses in Brazil...
January 11, 2017: BMC Medical Genomics
https://www.readbyqxmd.com/read/28068868/mir-26a-mediates-adipogenesis-of-amniotic-fluid-mesenchymal-stem-stromal-cells-via-pten-cyclin-e1-and-cdk6
#8
Ourania Trohatou, Dimitra Zagoura, Nikos Orfanos, Kalliopi I Pappa, Evangelos Marinos, Nicholas Anagnou, Maria G Roubelakis
Recent findings indicate that microRNAs (miRNAs) are critical for the regulatory network of adipogenesis in human mesenchymal stem/stromal cells (MSCs). Fetal MSCs derived from amniotic fluid (AF-MSCs) represent a population of multipotent stem cells characterized by a wide range of differentiation properties that can be applied in cell-based therapies. In this study, miRNA microarray analysis was performed to assess miRNA expression in terminal differentiated AF-MSCs into adipocyte-like cells (AL cells). MiR-26a was identified in high expression levels in AL cells indicating a critical role in the process of adipogenesis...
January 9, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28067049/amniotic-fluid-and-maternal-serum-metabolic-signatures-in-the-2nd-trimester-associated-with-pre-term-delivery
#9
Christina Virgiliou, Helen G Gika, Michael Witting, Anna A Bletsou, Apostolos Athanasiadis, Menelaos Zafrakas, Nikolaos S Thomaidis, Nikolaos Raikos, Georgios Makrydimas, Georgios A Theodoridis
Pre-term delivery (PTD) represents a major health problem which occurs in one in ten births. The hypothesis of the present study was that the metabolic profile of different biological fluids, obtained from pregnant women during the 2nd trimester of gestation, could allow useful correlations with pregnancy outcome. Holistic and targeted metabolomics approaches were applied for the complementary assessment of the metabolic content of prospectively collected amniotic fluid (AF) and paired maternal blood serum samples from 35 women who delivered preterm (between 29weeks+0days and 36weeks +5 days gestation) and 35 women delivered at term...
January 9, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28064548/amniotic-fluid-sludge-prevalence-and-clinical-significans-of-it-in-asymptomatic-patients-at-high-risk-for-spontaneous-preterm-delivery
#10
Ilknur Adanir, Ozgur Ozyuncu, Ayse Filiz Gokmen Karasu, Lutfu S Onderoglu
INTRODUCTION: The aim of our study is to determine prevalence and clinical significance of the presence of amniotic fluid 'sludge' among asymptomatic patients at high-risk for spontaneous preterm delivery, prospectively. MATERIAL AND METHODS: In our study, 99 patients at high risk for spontaneous preterm delivery were evaluated for the presence of amniotic fluid sludge with transvaginal ultrasonography at 20-22., 26-28. and 32-34. gestational weeks, prospectively; between August 2009 and October 2010 in Hacettepe University Hospital...
January 8, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28062958/a-study-of-oxidative-stress-in-neonates-delivered-through-meconium-stained-amniotic-fluid
#11
Tapas Bandyopadhyay, B D Bhatia, H D Khanna
: To estimate the levels of malondialdehyde (MDA) and 8-hydroxy-2-deoxyguanosine (8-OH-dG) in cord blood plasma of newborns born through meconium-stained amniotic fluid (MSAF) and also to find out the correlation between their levels with birth weight and gestation, we measured the cord blood plasma levels of MDA and 8-OH-dG in 59 newborns born through MSAF and 50 newborns born through clear liquor. The levels of cord blood plasma MDA and 8-OH-dG were significantly higher in full-term and late-preterm newborns born through MSAF...
January 6, 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/28062945/responses-of-human-neonates-to-highly-diluted-odorants-from-sweat
#12
Helene M Loos, Sébastien Doucet, Fanny Védrines, Constanze Sharapa, Robert Soussignan, Karine Durand, Paul Sagot, Andrea Buettner, Benoist Schaal
Conjugated forms of odorants contributing to sweat odor occur not only in human sweat but also in amniotic fluid, colostrum, and milk. However, it is unclear whether the released odorants are detected and hedonically discriminated by human newborns. To investigate this issue, we administered highly diluted solutions of (R)/(S)-3-methyl-3-sulfanylhexan-1-ol (MSH), (R)/(S)-3-sulfanylhexan-1-ol (SH), (E)/(Z)-3-methylhex-2-enoic acid (3M2H), and (R)/(S)-3-hydroxy-3-methylhexanoic acid (HMHA) to 3-d-old infants while their respiratory rate and oro-facial movements were recorded...
January 6, 2017: Journal of Chemical Ecology
https://www.readbyqxmd.com/read/28062025/amniotic-fluid-technical-update-on-physiology-and-measurement
#13
Kenneth I Lim, Kimberly Butt, Kentia Naud, Mila Smithies
OBJECTIVE: OUTCOMES: EVIDENCE: A MEDLINE and KFINDER search was used to identify relevant articles, with review of bibliography identified article including Cochrane reviews and recent review articles. VALUES: The evidence collected was reviewed by the Diagnostic Imaging Committee of the Society of Obstetricians and Gynecologists of Canada. The recommendations were made according to the guidelines developed by The Canadian Task Force on Preventative Health Care (Table 1)...
January 2017: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
https://www.readbyqxmd.com/read/28046155/anethol-cinnamaldehyde-and-eugenol-inclusion-in-feed-affects-postweaning-performance-and-feeding-behavior-of-piglets
#14
L Blavi, D Solà-Oriol, J J Mallo, J F Pérez
The early exposure of the fetus to certain volatiles may result in a further preference for these compounds later in life and could positively affect the acceptance of feed containing a similar flavor and the zootechnical responses. The study consisted of 2 trials to determine if including Fluidarom 1003 (a commercially flavored feed additive containing >25% anethol and cinnamaldehyde and >10% eugenol; Norel S.A., Madrid, Spain, Spain) in sow and postweaning piglet diets 1) provokes the presence or absence of 3 major volatile compounds (anethol, cinnamaldehyde, and eugenol) in amniotic fluid and milk, affecting piglet performance (BW, ADG, ADFI, and feed conversion ratio) after weaning, and 2) modifies creep feed consumption and feed preference in a 2-choice test...
December 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/28042936/effect-of-type-2-diabetes-mellitus-on-the-pharmacokinetics-and-transplacental-transfer-of-nifedipine-in-hypertensive-pregnant-women
#15
Gabriela Campos de Oliveira Filgueira, Osmany Alberto Silva Filgueira, Daniela Miarelli Carvalho, Maria Paula Marques, Elaine Christine Dantas Moisés, Geraldo Duarte, Vera Lucia Lanchote, Ricardo Carvalho Cavalli
AIMS: Diabetes mellitus can inhibit CYP3A4, an enzyme responsible for the metabolism of nifedipine, used for the treatment of hypertension in pregnant women. We aimed to assess the effect of type 2 diabetes mellitus (T2DM) on the pharmacokinetics, placental transfer and distribution of nifedipine in amniotic fluid in hypertensive pregnant women. METHODS: The study was conducted on 12 hypertensive pregnant women (control group - CG) and 10 hypertensive pregnant women with T2DM taking slow-release nifedipine (20 mg, 12/12 hours)...
January 2, 2017: British Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/28042787/-predictive-factors-of-the-outcomes-of-prenatal-hydronephrosis
#16
Paolo Bragagnini, Blanca Estors, Reyes Delgado, Miguel Ángel Rihuete, Jesús Gracia
OBJECTIVES: To determine prenatal and postnatal independent predictors of poor outcome, spontaneous resolution, or the need for surgery in patients with prenatal hydronephrosis. METHODS: We performed a retrospective study of patients with prenatal hydronephrosis. The renal pelvis APD was measured in the third prenatal trimester ultrasound, as well as in the first and second postnatal ultrasound. Other variables were taken into account, both prenatal and postnatal...
December 2016: Archivos Españoles de Urología
https://www.readbyqxmd.com/read/28040138/second-trimester-amniotic-fluid-uric-acid-potassium-and-cysteine-to-methionine-ratio-levels-as-possible-signs-of-early-preeclampsia-a-case-report
#17
Maria Fotiou, Alexandra-Maria Michaelidou, Sophia Masoura, Georgios Menexes, Vasiliki Koulourida, Costas G Biliaderis, Basil C Tarlatzis, Apostolos P Athanasiadis
OBJECTIVE: The precise etiopathogenesis of preeclampsia (PE) still remains enigmatic. In recent published work, there is a scientific trend aiming to unveil early biomarkers of PE based on amniotic fluid compositional changes before the development of clinical symptoms. CASE REPORT: We describe a case of an apparently clinically healthy woman, whose amniotic fluid, retrieved after amniocentesis at 22(2/7) gestational week, had elevated uric acid and potassium concentration, as well as cysteine to methionine ratio...
December 2016: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28039853/the-presence-of-vaginal-lactobacillus-species-does-not-contribute-to-a-measureable-difference-in-amniotic-fluid-lactate-levels-collected-from-the-vaginal-tract-of-laboring-women
#18
Beverley Hall, Diana Wong, Clare Healy, Mark B Tracy, Sally K Tracy, William D Rawlinson
Amniotic fluid lactate research is based on the hypothesis that a relationship exists between fatigued uterine muscles and raised concentrations of the metabolite lactate, which is excreted into the amniotic fluid during labor. To assess potentially confounding effects of lactate producing organisms on amniotic fluid lactate measurements, we aimed to determine if the presence of vaginal Lactobacillus species was associated with elevated levels of amniotic fluid lactate, measured from the vaginal tract of women in labor MATERIAL AND METHODS: Results from this study contribute to a large prospective longitudinal study of amniotic fluid lactate at a teaching hospital in Sydney, Australia...
December 31, 2016: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/28031434/testing-for-cmv-in-pregnancy
#19
Alda Saldan, Gabriella Forner, Carlo Mengoli, Nadia Gussetti, Giorgio Palù, Davide Abate
Congenital CMV-infection (cCMV) represents a relevant cause of deafness and neurological damage in newborns. Intrauterine CMV transmission might result after primary or non-primary infections, though at different rates (30% vs 0.2%, respectively). At present prenatal diagnosis of CMV infection is mainly based on maternal serology, detection of CMV-DNA in amniotic fluid and fetal blood, and US and MRI imaging. Recent evidences suggest that cCMV may be an immune-mediated disease and that evaluation of humoral and especially T-cell immunity may improve the overall prenatal diagnosis...
December 28, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28019126/mode-of-delivery-among-women-admitted-with-polyhydramnios
#20
Abeer Suleiman, Raed Salim
A retrospective matched case control study was conducted to examine the incidence of caesarean delivery (CD) among women admitted with polyhydramnios with and without a trial of labour compared to women with normal amniotic fluid index (AFI). Singleton pregnancies diagnosed with polyhydramnios upon admission to labour between 2003 and 2013 were included. A control group (normal AFI) matched at a ratio of 1:1 was randomly selected. Primary outcome was the incidence of CD. A total of 588 women were included. The overall incidence of CD was significantly higher among women with polyhydramnios (31...
December 26, 2016: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
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