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https://www.readbyqxmd.com/read/28523762/a-retrospective-study-of-cytogenetic-results-from-amniotic-fluid-in-5328-fetuses-with-abnormal-obstetric-sonographic-findings
#1
Shuo Zhang, Caixia Lei, Junping Wu, Haiyan Sun, Yuezhou Yang, Yueping Zhang, Xiaoxi Sun
OBJECTIVES: The purpose of this study was to evaluate the diagnostic utility of karyotype analysis of amniotic fluid for fetuses with abnormal sonographic findings and to determine the detection rates of abnormal karyotypes. METHODS: We conducted a retrospective study of 5328 fetuses with abnormal sonographic findings in the first or second trimester enrolled from October 1998 and September 2015. Cytogenetic results from amniotic fluid were obtained in all of these pregnancies...
May 19, 2017: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/28520465/mesenchymal-stem-stromal-cells-in-regenerative-medicine-past-present-and-future
#2
Ourania Trohatou, Maria G Roubelakis
The concept of Regenerative Medicine combined with Cell based Therapy and Tissue Engineering represents the fourth pillar of healthcare and provides a promising approach for the treatment of serious diseases. Recently, cell based therapies are focused on the use of mesenchymal stem/stromal cells (MSCs). Human MSCs, that represent a mesoderm derived population of progenitors, are easily expanded in culture. They are capable to differentiate into osteoblasts, chondrocytes, and adipocytes and exhibit the potential to repair or regenerate damaged tissues...
May 18, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28508565/menaquinone-4-enhances-osteogenic-potential-of-human-amniotic-fluid-mesenchymal-stem-cells-cultured-in-a-2d-and-3d-dynamic-culture-system
#3
Domitilla Mandatori, Letizia Penolazzi, Caterina Pipino, Pamela Di Tomo, Sara Di Silvestre, Natalia Di Pietro, Sara Trevisani, Marco Angelozzi, Mariangela Ucci, Roberta Piva, Assunta Pandolfi
Menaquinones, also known as Vitamin K2 family, regulate calcium homeostasis in a "bone-vascular cross talk" and recently received particular attention for their positive effect on bone formation. Since the correlation between menaquinones and bone metabolism to date is still unclear, the objective of our study was to investigate the possible role of menaquinone-4 (MK-4), an isoform of the menaquinones family, in the modulation of osteogenesis. For this reason we used a model of human Amniotic Fluid Mesenchymal Stem Cells (hAFMSCs) cultured both in two-dimensional (2D) and three-dimensional (3D, RCCS™bioreactor) in vitro culture systems...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28503900/effects-of-in-ovo-feeding-of-l-arginine-on-the-development-of-digestive-organs-intestinal-function-and-post-hatch-performance-of-broiler-embryos-and-hatchlings
#4
T Gao, M M Zhao, Y J Li, L Zhang, J L Li, L L Yu, F Gao, G H Zhou
This study was to investigate the effects of in ovo feeding (IOF) L-arginine (Arg) solution on the development of digestive organs, the duodenal mucosa of broiler embryos and hatchlings, and the growth performance of chicks during the first week post-hatch. A total of 720 fertilized eggs with similar weight were randomly allocated to three groups, consisting of eight replicates of 30 eggs each. Three treatments were arranged as non-injected control, diluent-injected (0.75% NaCl solution) group and Arg solution-injected group containing 1% Arg, dissolved in diluent...
May 15, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28503847/addition-of-color-doppler-sonography-for-detection-of-amniotic-fluid-disturbances-and-its-implications-on-perinatal-outcomes
#5
Imelda N Odibo, Brianna S Whittemore, Dawn S Hughes, Pamela M Simmons, Songthip T Ounpraseuth, Everett F Magann
OBJECTIVES: To determine whether color Doppler sonography increases the detection of pregnancies at risk for adverse outcomes. METHODS: Participants admitted to labor and delivery with the anticipation of a vaginal delivery underwent measurements of amniotic fluid volume (AFV) using amniotic fluid index (AFI) and single deepest pocket (SDP) techniques by grayscale followed by color Doppler sonography. Oligohydramnios was defined as an AFI of less than 5 cm or an SDP of less than 2 cm...
May 15, 2017: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/28503779/normal-amniotic-fluid-volume-across-gestation-comparison-of-statistical-approaches-in-1190-normal-amniotic-fluid-volumes
#6
Songthip T Ounpraseuth, Everett F Magann, Horace J Spencer, Nader Z Rabie, Adam T Sandlin
AIM: Ultrasound estimation and evaluation of amniotic fluid volume (AFV) is an important component of pregnancy surveillance and fetal well-being. The purpose of this study was to compare and contrast four statistical methods used to construct gestational age-specific reference intervals for the assessment of AFV. METHODS: A total of 1095 normal AFV derived from four studies that measured AFV using dye-dilution or direct measurement at the time of hysterotomy were used to construct reference intervals using polynomial regression, quantile regression, Royston and Wright mean and SD, and Cole's lambda mu sigma (LMS) methods...
May 15, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28502201/prediction-of-chorioamnionitis-in-cases-of-intraamniotic-infection-by-ureaplasma-urealyticum-in-women-with-very-preterm-premature-rupture-of-membranes-or-preterm-labour
#7
Rocio Revello, María José Alcaide, Daniel Abehsera, María Martin-Camean, Mafalda Sousa E Faro Gomes, Bárbara Alonso-Luque, Jose L Bartha
OBJECTIVES: First, to determinate the frequency of chorioamnionitis and funisitis in cases of intramniotic detection of Ureaplasma urealyticum. And second, to assess the predictive capability of some biological markers in the amniotic fluid of these women to predict histological inflammation. SUBJECTS AND METHODS: We prospectively studied 20 cases of women with premature rupture of membranes or preterm labour (PROM) or preterm labour and intraamniotic detection of Ureaplasma urealyticum...
May 14, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28499502/sesamin-encouraging-effects-on-chondrogenic-differentiation-of-human-amniotic-fluid-derived-mesenchymal-stem-cells
#8
Suteera Narakornsak, Sirinda Aungsuchawan, Peeraphan Pothacharoen, Runchana Markmee, Waleephan Tancharoen, Tanongsak Laowanitwattana, Chawapon Thaojamnong, Lamaiporn Peerapapong, Nonglak Boonma, Witoon Tasuya, Junjira Keawdee, Naree Poovachiranon
Worldwide, the most recognized musculoskeletal degenerative disease is osteoarthritis (OA). Sesamin, a major abundant lignan compound present in Sesamun Indicum Linn, has been described for its various pharmacological effects and health benefits. However, the promoting effects of sesamin on chondrogenic differentiation have not yet been observed. Herein, the aim of this study was to investigate the effects of sesamin on cell cytotoxicity and the potent supporting effects on chondrogenic differentiation of human amniotic fluid-derived mesenchymal stem cells (hAF-MSCs)...
May 9, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28499241/pathophysiology-mechanisms-and-applications-of-mesenchymal-stem-cells-for-the-treatment-of-spinal-cord-injury
#9
REVIEW
Pravin Shende, Muna Subedi
Spinal Cord Injury (SCI) is a serious devastating condition associated to the high chances of morbidity and mortality. It involves a primary and a secondary injury, former cause damages to both lower and upper motor neurones and disrupts sensory, motor and autonomic functions while the latter involves various stages of molecular plus cellular incidents which elaborate the original injury. In the treatment of SCI, stem cells possess a good therapeutic potential. Bone marrow, adipose tissue, placenta, amniotic fluid and umbilical cord are the good sources for mesenchymal stem cells...
May 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28487016/the-expression-level-of-c19mc-mirnas-in-early-pregnancy-and-in-response-to-viral-infection
#10
Tina M F Dumont, Jean-Francois Mouillet, Avaraham Bayer, Christina L Gardner, William B Klimstra, Dana G Wolf, Simcha Yagel, Fabiola Balmir, Anna Binstock, Joseph S Sanfilippo, Carolyn B Coyne, Jacob C Larkin, Yoel Sadovsky
INTRODUCTION: We have previously shown that miRNAs produced from the Chromosome 19 MiRNA Cluster (C19MC), which are expressed almost exclusively in primate trophoblasts and are released into the maternal circulation, reduce viral replication in non-placental cells and can modulate migratory behavior of extravillous trophoblast. We sought to define the expression pattern of C19MC miRNA in early pregnancy and in response to viral infection in vitro and in vivo. METHODS: We prospectively followed women undergoing in vitro fertilization (IVF) and determined their blood level of C19MC miRNA using RT-qPCR...
May 2017: Placenta
https://www.readbyqxmd.com/read/28484292/correlation-of-oxidative-stress-markers-with-ultrasound-and-cardiotocography-parameters-with-hypertension-induced-pregnancy
#11
Dragica Draganovic, Nenad Lucic, Dragica Jojic, Snjezana Milicevic
INTRODUCTION: Pregnancy induced hypertension (PIH), especially preeclampsia, is a state of extremely increased oxidative stress (OS), due to decrease of antioxidant capacity in comparison to normotensive pregnant women. The consequences of increased state of oxidative stress with hypertension disorder in pregnancy are disorder of placental blood flow, intrauterine hypoxia of the fetus and disturbance in transfer of O2 manifested as pathological ultrasound (US) parameters (amount of amniotic fluid and biophysical profile of fetus) and cardiotocography parameters (CTG)...
March 2017: Acta Informatica Medica: AIM
https://www.readbyqxmd.com/read/28482121/cell-therapy-for-stress-urinary-incontinence-present-day-frontiers
#12
REVIEW
Andrey Vinarov, Anthony Atala, James Yoo, Roman Slusarenco, Marat Zhumataev, Alexey Zhito, Denis Butnaru
CONTEXT: Stress urinary incontinence (SUI) significantly diminishes the quality of patients' lives. Currently available surgical and nonsurgical therapies remain far from ideal. At present advancements in cellular technologies have stirred growing interest in the use of autologous cell treatments aimed to regain urinary control. OBJECTIVE: To conduct a review of the literature and analyze preclinical and clinical studies dedicated to various cell therapies for SUI, assessing their effectiveness, safety, and future prospects...
May 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28476482/report-on-the-society-for-fetal-urology-panel-discussion-on-the-selection-criteria-and-intervention-for-fetal-bladder-outlet-obstruction
#13
M K Farrugia, M C Braun, C A Peters, R Ruano, C D Herndon
INTRODUCTION: The Society for Fetal Urology panel section at the 2016 Fall Congress featured a multidisciplinary discussion on appropriate patient selection, the conservative versus surgical management, and postnatal renal outcome of fetuses with lower urinary tract obstruction (LUTO). SELECTION CRITERIA FOR INTERVENTION: Rodrigo Ruano shared his experience of prenatal intervention, presenting the outcome of 111 fetuses with severe LUTO treated with vesicoamniotic shunting (VAS) (n = 16), cystoscopy (n = 34) or no intervention (n = 61) in a non-randomized series...
March 24, 2017: Journal of Pediatric Urology
https://www.readbyqxmd.com/read/28469780/the-relationship-between-amniotic-fluid-mirnas-and-congenital-obstructive-nephropathy
#14
Juntao Xie, Yi Zhou, Wenzong Gao, Zuoqing Li, Zhe Xu, Li Zhou
Exosomes are small membrane vesicles with size of 30-100 nm, which were found in bodily fluids including amniotic fluid and saliva. The biological materials in exosomes, such as proteins and RNA, can be used as novel potential biomarkers for diagnostic assays. The purpose of this study was to assess whether exosomal microRNAs (miRNAs) could be used as biomarkers to prenatally diagnose congenital hydronephrosis and to evaluate fetal kidney function. Transmission electron microscopy (TEM), flow cytometry (FACS), and western-blot were applied to identify exosomes in the amniotic fluid from fetuses with congenital hydronephrosis and healthy controls...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469527/histologic-chorioamnionitis-amniotic-fluid-interleukin-6-krebs-von-den-lungen-6-and-transforming-growth-factor-%C3%AE-1-for-the-development-of-neonatal-bronchopulmonary-dysplasia
#15
Hisako Matsumura, Hiroyuki Ichiba, Satoshi Ohnishi, Mika Saito, Haruo Shintaku
BACKGROUND: Chorioamnionitis (CAM) is an important risk factor for the development of bronchopulmonary dysplasia (BPD) in preterm infants. OBJECTIVES: To evaluate the effects of CAM on the development of BPD using interleukin 6 (IL-6), Krebs von den Lungen 6 (KL-6), and transforming growth factor β1 (TGF-β1) in the amniotic fluid as markers for inflammation, lung injury, and fibrosis/remodeling, respectively. METHODS: Amniotic fluid concentrations of IL-6, KL-6, and TGF-β1 were measured with enzyme-linked immunosorbent assay or electro-chemiluminescence immunoassay...
2017: Japanese Clinical Medicine
https://www.readbyqxmd.com/read/28464190/amniotic-fluid-embolism-is-a-new-pregnancy-possible-case-report
#16
Ana Filipa Cabrita Caeiro, Irina Dulce Tapadinhas Matos Ramilo, Ana Paula Santos, Elizabeth Ferreira, Isabel Santos Batalha
Amniotic fluid embolism (AFE) is a rare but potentially catastrophic clinical condition, characterized by a combination of signs and symptoms that reflect respiratory distress, cardiovascular collapse and disseminated intravascular coagulation (DIC). Its pathogenesis is still unclear. More recently, the traditional view of obstruction of pulmonary capillary vessels by amniotic fluid emboli as the main explanation for the etiology has been ruled out, and immunologic factors and the activation of the inflammatory cascade took on an important role...
May 2, 2017: Revista Brasileira de Ginecologia e Obstetrícia
https://www.readbyqxmd.com/read/28460634/acute-kidney-injury-during-pregnancy-and-puerperium-a-retrospective-study-in-a-single-center
#17
Chunhong Huang, Shanying Chen
BACKGROUND: Acute kidney injury (AKI) is rare in women during pregnancy and puerperium, however, it is related to increased morbidity and mortality rates. OBJECTIVE: The aim of this study was to investigate the incidence, characteristics, and outcomes of AKI during pregnancy and puerperium in a Chinese population. METHODS: In this study, pregnant women discharged from hospital between January 2008 and June 2015 were screened. AKI was defined if the level of serum creatitine >70...
May 1, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/28459702/report-of-a-triploid-fetus-identified-in-a-pregnancy-with-oligohydramnios
#18
Ghanbarian Alavijeh Maryam, Saber Siamak, Fazeli Bavand-Pour Fatemeh Sadat, Mirzaloo Masoud, Mirzaie Fatemeh, Zare-Abdollahi Davood, Ebrahimi Ahmad
This report describes a pregnancy with a triploid fetus identified from a scan for anomalies at 18 weeks and confirmed by amniocentesis. A 29-year-old, primigravida woman was referred to our clinic for genetic counseling at 18 weeks of gestation because of a mild oligohydramnios due to amniotic fluid index (AFI) less than the fifth percentile in her 18th week. The woman underwent amniocentesis, which revealed a karyotype of 69,XXX. There was no consanguinity in this family. In postmortem evaluation, we encountered a hydrocephalic fetus with an aberrant skull shape, including dysplastic calvaria with large posterior fontanel, marked intrauterine growth retardation (IUGR) in addition to syndactyly of third and fourth fingers, exophthalmia, cleft palate, low set malformed ears, micrognathia and club foot...
2017: Journal of the Association of Genetic Technologists
https://www.readbyqxmd.com/read/28455084/recipient-umbilical-artery-elongation-redundancy-in-twin-twin-transfusion-syndrome
#19
Roopali Donepudi, Lovepreet K Mann, Christoph Wohlmuth, Anthony Johnson, Michael W Bebbington, Kenneth J Moise, David S Boudreaux, Helena Gardiner, Ramesha Papanna
BACKGROUND: Chronic hypertension in adults causes arterial lengthening in major arteries, but the effects of early fetal hypertension on the twin-twin transfusion syndrome (TTTS) recipient's vascular architecture remains unknown. OBJECTIVES: We hypothesize that arterial cord redundancy is related to recipient hypertension and subsequent heart failure. Our objectives were: 1) to establish a 3D color Doppler ultrasound method of measuring umbilical arterial (UA) length relative to its corresponding venous segment in the Umbilical cord using AV-angle, 2) to compare recipient AV-angle to gestational age-matched controls, and 3) to test the association of AV-angle with recipient heart failure...
April 25, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28454555/a-critical-assessment-of-the-sterile-womb-and-in-utero-colonization-hypotheses-implications-for-research-on-the-pioneer-infant-microbiome
#20
REVIEW
Maria Elisa Perez-Muñoz, Marie-Claire Arrieta, Amanda E Ramer-Tait, Jens Walter
After more than a century of active research, the notion that the human fetal environment is sterile and that the neonate's microbiome is acquired during and after birth was an accepted dogma. However, recent studies using molecular techniques suggest bacterial communities in the placenta, amniotic fluid, and meconium from healthy pregnancies. These findings have led many scientists to challenge the "sterile womb paradigm" and propose that microbiome acquisition instead begins in utero, an idea that would fundamentally change our understanding of gut microbiota acquisition and its role in human development...
April 28, 2017: Microbiome
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