keyword
MENU ▼
Read by QxMD icon Read
search

Cdk2

keyword
https://www.readbyqxmd.com/read/28732387/the-micrornas-mir-200b-3p-and-mir-429-5p-target-the-limk1-cfl1-pathway-to-inhibit-growth-and-motility-of-breast-cancer-cells
#1
Dengfeng Li, Hong Wang, Hongming Song, Hui Xu, Bingkun Zhao, Chenyang Wu, Jiashu Hu, Tianqi Wu, Dan Xie, Junyong Zhao, Qiang Shen, Lin Fang
Triple-negative breast cancer (TNBC) has the worst prognosis of all subtypes of breast cancer (BC), with limited options for conventional therapy and no targeted therapies. MicroRNAs (miRNAs) are small noncoding RNAs that negatively regulate gene expression. In this study, we aimed to determine whether two members of the miR-200 family, miR-200b-3p and miR-429-5p, are involved in BC cell proliferation and motility and to elucidate their target genes and pathways. We performed a meta-analysis that reveals down-regulated expression of miR-200b-3p and miR-429-5p in BC tissues and cell lines, consistent with a lower expression of miR-200b-3p and miR-429-5p in MDA-MB-231 and HCC1937 cells than in MCF-7 and MCF-10 cells...
July 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/28732361/morusin-inhibits-cell-proliferation-and-tumor-growth-by-downregulating-c-myc-in-human-gastric-cancer
#2
Feng Wang, Dunke Zhang, Jingxin Mao, Xiao-Xue Ke, Rui Zhang, Chao Yin, Ning Gao, Hongjuan Cui
Morusin is a pure extract from the root bark of Morus australis (Moraceae). In recent years, morusin has been reported to exhibit anti-tumor biological activity in some types of human cancers through different mechanisms. Here, we attempted to investigate the inhibitory effect and mechanism of morusin on gastric cancer. Morusin markedly inhibited gastric cancer cell proliferation by down-regulating CDKs and Cyclins, such as CDK2, CDK4, Cyclin D1 and Cyclin E1. Additionally, morusin suppressed tumor growth in vitro and in vivo...
July 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28730478/high-content-imaging-and-rnai-screens-for-investigating-kinase-network-plasticity
#3
Simon R Stockwell, Sibylle Mittnacht
High-content imaging connects the information-rich method of microscopy with the systematic objective principles of software-driven analysis. Suited to automation and, therefore, considerable scale-up of study size, this approach can deliver multiparametric data over cell populations or at the level of the individual cell and has found considerable utility in reverse genetic and pharmacological screens. Here we present a method to screen small interfering RNA (siRNA) libraries allowing subsequent observation of the impact of each knockdown on two interlinked, high-content, G1-/S-phase cell cycle transition assays related to cyclin-dependent kinase (CDK) 2 activity...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28727572/classical-swine-fever-virus-shimen-infection-increases-p53-signaling-to-promote-cell-cycle-arrest-in-porcine-alveolar-macrophages
#4
Pengbo Ning, Congxia Hu, Xuepeng Li, Yulu Zhou, Aoxue Hu, Ya Zhang, Lifang Gao, Cunmei Gong, Kangkang Guo, Xianghan Zhang, Yanming Zhang
Classical swine fever virus (CSFV) replicates in macrophages and causes persistent infection. Despite its role in disastrous economic losses in swine industries, the molecular mechanisms underlying its pathogenesis are poorly understood. The virus evades the neutralizing immune response, subverting the immune system to ensure its own survival and persistence.Our genome-wide analysis of porcine alveolar macrophage transcriptional responses to CSFV Shimen infection using the Solexa/Illumina digital gene expression system revealed that p53 pathway components and cell cycle molecules were differentially regulated during infection compared to controls...
July 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28726723/mertensene-a-halogenated-monoterpene-induces-g2-m-cell-cycle-arrest-and-caspase-dependent-apoptosis-of-human-colon-adenocarcinoma-ht29-cell-line-through-the-modulation-of-erk-1-2-akt-and-nf-%C3%AE%C2%BAb-signaling
#5
Safa Tarhouni-Jabberi, Ons Zakraoui, Efstathia Ioannou, Ichrak Riahi-Chebbi, Meriam Haoues, Vassilios Roussis, Riadh Kharrat, Khadija Essafi-Benkhadir
Conventional treatment of advanced colorectal cancer is associated with tumor resistance and toxicity towards normal tissues. Therefore, development of effective anticancer therapeutic alternatives is still urgently required. Nowadays, marine secondary metabolites have been extensively investigated due to the fact that they frequently exhibit anti-tumor properties. However, little attention has been given to terpenoids isolated from seaweeds. In this study, we isolated the halogenated monoterpene mertensene from the red alga Pterocladiella capillacea (S...
July 20, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28723151/fusion-of-structure-and-ligand-based-methods-for-identification-of-novel-cdk2-inhibitors
#6
Priya Mahajan, Gousia Chashoo, Monika Gupta, Amit Kumar, Parvinder Pal Singh, Amit Nargotra
Cyclin dependent kinases plays a central role in cell cycle regulation which makes them a promising target with multifarious therapeutic potential. CDK2 regulates various events of the eukaryotic cell division cycle and the pharmacological evidences indicated that over expression of CDK2 causes abnormal cell-cycle regulation, which was directly associated with hyper proliferation of cancer cells. Therefore, CDK2 is regarded as a potential target molecule for anti-cancer medication. Thus to decline CDK2 activity by potential lead compounds has proved to be an effective treatment for cancer...
July 19, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28720813/a-new-prenylated-flavonoid-induces-g0-g1-arrest-and-apoptosis-through-p38-jnk-mapk-pathways-in-human-hepatocellular-carcinoma-cells
#7
Di Wang, Qian Sun, Jie Wu, Wei Wang, Guodong Yao, Tianyang Li, Xue Li, Lingzhi Li, Yan Zhang, Wei Cui, Shaojiang Song
Prenylated flavonoids have been demonstrated to possess diverse bioactivities including antitumor effects. One new, daphnegiravone D (1), and four known (2-5) prenylated flavonoids were isolated from Daphne giraldii. Their cytotoxic activities revealed that daphnegiravone D markedly inhibited the proliferation of cancer cells, but had no apparent cytotoxicity on human normal cells. Mechanistically, daphnegiravone D induced G0/G1 arrest and apoptosis, reduced the expression of cyclin E1, CDK2 and CDK4, and promoted the cleavage of caspase 3 and PARP in Hep3B and HepG2 cells...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28718667/hydroxytyrosol-induces-apoptosis-and-cell-cycle-arrest-and-suppresses-multiple-oncogenic-signaling-pathways-in-prostate-cancer-cells
#8
Haseeb Zubair, Arun Bhardwaj, Aamir Ahmad, Sanjeev Kumar Srivastava, Mohammad Aslam Khan, Girijesh Kumar Patel, Seema Singh, Ajay Partap Singh
SCOPE: Hydroxytyrosol (HT), a polyphenol from olives, is a potential anticancer agent. This study was designed to evaluate the anticancer activity of HT against prostate cancer cells, and the mechanism thereof. METHODS AND RESULTS: Treatment of LNCaP and C4-2 prostate cancer cells with HT resulted in a dose-dependent inhibition of proliferation. This was in contrast to HT's ineffectiveness against normal prostate epithelial cells RWPE1 and PWLE2, suggesting cancer-cell-specific effect...
July 18, 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28716710/microrna-143-acts-as-a-suppressor-of-hemangioma-growth-by-targeting-bcl-2
#9
Chongqing Huang, JingYong Huang, Pengyan Ma, Guanfeng Yu
Infantile hemangioma is the most common vascular tumor affecting infants, which is associated with clonal expansion of endothelial cells. The aim of this study is to determine the role of microRNA (miR)-143 in the growth and survival of hemangioma-derived endothelial cells (HemECs). We examined the expression of miR-143 in patients with proliferating-phase (n=10) and involuting-phase (n=8) hemangiomas. The effects of ectopic expression of miR-143 on the viability, proliferation, cell cycle distribution, and apoptosis of HemECs were explored...
July 14, 2017: Gene
https://www.readbyqxmd.com/read/28713151/trifluoperazine-inhibits-mesangial-cell-proliferation-by-arresting-cell-cycle-dependent-mechanisms
#10
Baodong Wang, Xiaoshuang Zhou, Yanqin Wang, Rongshan Li
BACKGROUND It has been reported that trifluoperazine (TFP) inhibits proliferation of cancer cells, however, the effects of TFP in renal proliferation diseases are still unclear. This study examined the effects of TFP on proliferation of human renal mesangial cells and analyzed the underlying mechanisms. MATERIAL AND METHODS Cell proliferation in vivo was determined by HE staining, immunohistochemistry of proliferating cell nuclear antigen (PCNA), and Western blot analysis (Ki-67 and PCNA). Effects of different TFP concentrations and treatment duration on cell proliferation and cell cycle were analyzed using the MTT assay and flow cytometry...
July 17, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28711931/apc-c-is-essential-for-hematopoiesis-and-impaired-in-aplastic-anemia
#11
Jia Wang, Min-Zhi Yin, Ke-Wen Zhao, Fang Ke, Wen-Jie Jin, Xiao-Lin Guo, Tian-Hui Liu, Xiao-Ye Liu, Hao Gu, Xiao-Min Yu, Zhen Li, Li-Li Mu, Deng-Li Hong, Jing Chen, Guo-Qiang Chen
Anaphase promoting complex/cyclosome (APC/C) is essential for cell cycle progression. Recently, its non-mitotic functions were also reported but less studied in several tissues including hematopoietic cells. Here, we developed an inducible Anapc2 (a core subunit of APC/C) knockout mice. The animals displayed a fatal bone marrow failure within 7 days after knockout induction. Their hematopoietic stem and progenitor cells (HSPCs) demonstrated a sharp decline and could form little colony. Further, the results of BrdU label-retaining cell assay showed that the dormant HPSCs lost rapidly...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28710039/cloning-expression-pattern-and-potential-role-of-apoptosis-inhibitor-5-in-the-termination-of-embryonic-diapause-and-early-embryo-development-of-artemia-sinica
#12
Shuang Zhang, Feng Yao, Ting Jing, Mengchen Zhang, Wei Zhao, Xiangyang Zou, Linlin Sui, Lin Hou
During the embryonic development of Artemia sinica, the diapause phenomenon can be induced by high salinity or low temperature conditions. The diapause embryo at the gastrula stage is maintained under the threat of apoptosis to guarantee the embryo's normal development. In this process, apoptosis inhibitor proteins play vital roles in protecting embryos against apoptosis. Apoptosis inhibitor5 (API5) plays a pivotal role in regulating the cell cycle and preventing programmed cell death after growth factor starvation...
July 11, 2017: Gene
https://www.readbyqxmd.com/read/28708871/ursodeoxycholic-acid-inhibits-the-proliferation-of-colon-cancer-cells-by-regulating-oxidative-stress-and-cancer-stem-like-cell-growth
#13
Eun-Kyung Kim, Jae Hee Cho, EuiJoo Kim, Yoon Jae Kim
INTRODUCTION: The regulation of reactive oxygen species (ROS) exists as a therapeutic target for cancer treatments. Previous studies have shown that ursodeoxycholic acid (UDCA) suppresses the proliferation of colon cancer cells. The aim of this study was to evaluate the effect of UDCA upon the proliferation of colon cancer cells as a direct result of the regulation of ROS. METHOD: Colon cancer cell lines (HT29 and HCT116) were treated with UDCA. The total number of cells and the number of dead cells were determined using cell counters...
2017: PloS One
https://www.readbyqxmd.com/read/28704759/novel-pyrazolopyrimidines-synthesis-in%C3%A2-vitro-cytotoxic-activity-and-mechanistic-investigation
#14
Ghaneya S Hassan, Doaa E Abdel Rahman, Yassin M Nissan, Esraa A Abdelmajeed, Tamer M Abdelghany
A series of novel pyrazolo[3,4-d]pyrimidines bearing benzenesulfonamide moiety 5a-f, 6 and 7 were synthesized. Cytotoxic screening was conducted against MCF-7 and HepG2. 6-(4-Methoxyphenyl)-4-oxopyrazolopyrimidine derivative 5e and 4-imino-6-oxopyrazolopyrimidine derivative 6 revealed potent cytotoxic activity with IC50 1.4 μM (MCF-7) and 0.4 μM (HepG2), respectively compared to that of doxorubicin, (IC50 = 1.02 μM and 0.9 μM, respectively). Compounds 5e and 6 were subjected to cell cycle analysis and apoptosis assay after 24 h and 48 h treatment...
July 3, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28701787/avian-reovirus-p17-and-%C3%AF-a-act-cooperatively-to-downregulate-akt-by-suppressing-mtorc2-and-cdk2-cyclin-a2-and-upregulating-proteasome-psmb6
#15
Wei-Ru Huang, Pei-I Chi, Hung-Chuan Chiu, Jue-Liang Hsu, Brent L Nielsen, Tsai-Ling Liao, Hung-Jen Liu
Although we have shown that avian reovirus (ARV) p17-mediated inhibition of Akt leads to induction of autophagy, the precise mechanisms remain largely unknown. This study has identified a specific mechanism by which ARV coordinately regulates the degradation of ribosomal proteins by p17-mediated activation of E3 ligase MDM2 that targets ribosomal proteins and by σA-mediated upregulation of proteasome PSMB6. In addition to downregulating ribosomal proteins, p17 reduces mTORC2 assembly and disrupts mTORC2-robosome association, both of which inactivate mTORC2 leading to inhibition of Akt phosphorylation at S473...
July 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28693202/ppeop-from-omphalia-lapidescens-schroet-induces-cell-cycle-arrest-and-inhibits-the-migration-of-mc-4-gastric-tumor-cells
#16
Yong-Le Yang, Wei-Yao Gong, Fei-Fei Chen, Lu-Chao Chen, Yi-Tao Chen
The aim of the present study was to investigate the effect of purified Omphalia lapidescens protein (pPeOp) extracted by polyvinylpyrrolidone from the fungus Omphalia lapidescens Schroet on the proliferation and cell cycle progression of MC-4 human gastric tumor cells. Using polyvinylpyrrolidone, pPeOp was extracted from O. lapidescens Schroet. MC-4 cells were cultured with 30, 60 or 90 µg/ml pPeOp, with 5-fluorouracil used as a positive control. Survival rates of treated cells were significantly decreased compared with those of the untreated control group in a dose-dependent manner...
July 2017: Oncology Letters
https://www.readbyqxmd.com/read/28693160/isoliquiritigenin-induces-apoptosis-of-human-bladder-cancer-t24-cells-via-a-cyclin-dependent-kinase-independent-mechanism
#17
Lingling Si, Xinhui Yang, Xinyan Yan, Yanming Wang, Qiusheng Zheng
The aim of the present study was to investigate whether an increase in cyclin-dependent kinase 2 (CDK2) activity is involved in apoptosis of human bladder cancer T24 cells induced by isoliquiritigenin (ISL). The viability of T24 cells was estimated using a sulforhodamine B assay. Cell morphological changes were examined using Hoechst 33258 staining. The apoptotic rate was determined by staining cells with Annexin V-fluorescein isothiocyanate and propidium iodide labeling. The mitochondrial membrane potential (ΔΨm) was measured using 5,5,6,6-tetrachloro-1,1, 3,3-tetraethyl benzimidazole carbocyanine iodide...
July 2017: Oncology Letters
https://www.readbyqxmd.com/read/28684297/curcumol-induces-cell-cycle-arrest-in-colon-cancer-cells-via-reactive-oxygen-species-and-akt-gsk3%C3%AE-cyclin-d1-pathway
#18
Juan Wang, Xu-Mei Li, Zhun Bai, Bi-Xia Chi, Yan Wei, Xu Chen
ETHNOPHARMACOLOGICAL RELEVANCE: Curcuma kwangsiensis S. G. Lee & C. F. Liang (Guangxi ezhu, in Chinese) belongs to the Zingiberaceae family, has been used as a traditionally Chinese medicine nearly 2,000 year. Curcumol is one of the guaiane-type sesquiterpenoid hemiketal isolated from medicine plant Curcuma kwangsiensis S. G. Lee & C. F. Liang, which has been reported possesses anti-cancer effects. Our previous study found that the most contribution to inhibit nasopharyngeal carcinoma cell growth was curcumol...
July 3, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28678584/inhibition-of-cdk-mediated-smad3-phosphorylation-reduces-the-pin1-smad3-interaction-and-aggressiveness-of-triple-negative-breast-cancer-cells
#19
Alexandra L Thomas, Hanne Lind, Angela Hong, Danijela Dokic, Kailey Oppat, Elana Rosenthal, Amina Guo, Aaron Thomas, Randala Hamden, Jacqueline S Jeruss
Triple negative breast cancer (TNBC) is a highly aggressive breast cancer subtype that lacks effective targeted therapies. Although TNBC is not defined by specific therapeutic targets, a subset of patients have tumors that overexpress cyclins. High cyclin D/E expression catalyzes CDK4/2 activity. In turn, CDK4/2 can non-canonically phosphorylate Smad3, a key TGFβ signaling intermediate, and this phosphorylation has been associated with the shift from tumor-suppressive to oncogenic TGFβ pathway action in breast oncogenesis...
July 5, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28677808/a-novel-engineered-interferon-%C3%AE-hybrid-molecule-increases-anticancer-efficacy-of-doxorubicin-in-breast-cancer-chemotherapy
#20
Si-Jie Li, Chun-Shui Liu, Hai-Jun Li, Yan Li, Lei Zhou, Jin-Cheng Li, Yi-Chang Chen, Tian-Qi Su, De-Hai Yu
Breast cancer is the most common carcinoma among Chinese women. Interferon α (IFNα) has been used to treat various types of cancer, including breast cancer, but its antitumor activity is relative low, which significantly hinders its clinical application. In this study, we utilized a Ph.D.-12 peptide library screening system to identify a short peptide that specifically binds to MCF-7 breast cancer cells. By fusing the MCF-7 binding peptide (MBP) to the C-terminus of IFNα, we constructed an engineered IFNα-MBP fusion molecule (IMBP), and applied this novel fusion protein to the treatment of breast cancer...
July 3, 2017: International Journal of Oncology
keyword
keyword
57351
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"