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Sushant B Jadhav, Rahul M Mane, Kalyanraman L Narayanan, Popatrao N Bhosale
A novel, stability indicating, reverse phase high-performance liquid chromatography (RP-HPLC) method was developed to determine the S-isomer of linagliptin (LGP) in linagliptin and metformin hydrochloride (MET HCl) tablets (LGP-MET HCl) by implementing design of experiment (DoE), i.e., two-level, full factorial design (2³ + 3 centre points = 11 experiments) to understand the critical method parameters (CMP) and its relation with the critical method attribute (CMA), and to ensure robustness of the method. The separation of the S-isomer, LGP and MET HCl in the presence of their impurities was achieved on Chiralpak(®) IA-3 (Amylose tris (3, 5-dimethylphenylcarbamate), immobilized on 3 µm silica gel) stationary phase (250 × 4...
October 17, 2016: Scientia Pharmaceutica
Elisabeth Sterrer, Franz Windisch, Kurt Frey, Klaus Rettensteiner, Benjamin Loader
BACKGROUND: Middle ear adenoma with neuroendocrine differentiation (MEA-ND), also known as carcinoid tumor of the middle ear, is an extremely rare neoplasm, especially when located within the tympanic cavity. To the best of our knowledge, this represents the first pediatric case of a MEA-ND described in Europe. Excluding the present case, only three other pediatric cases of this disease have been published globally. CASE REPORT: We report on a 15-year-old female patient who presented with conductive hearing loss of the right ear...
October 19, 2016: Wiener Klinische Wochenschrift
P Gal, T J Buist, J J J Smit, A Adiyaman, A R Ramdat Misier, P P H M Delnoy, A Elvan
INTRODUCTION: Pulmonary vein (PV) reconnection is frequently the cause of recurrence of atrial fibrillation (AF) after ablation. The second-generation gold multi-electrode ablation (Gold-MEA) catheter has a new design possibly resulting in improved lesion formation compared with its predecessor. We aimed to determine the association between effective radiofrequency applications with the Gold-MEA catheter and outcome after AF ablation. METHODS: 50 consecutive patients with paroxysmal AF underwent Gold-MEA (PVAC GOLD(TM), Medtronic Inc...
October 17, 2016: Netherlands Heart Journal
Sangobtip Pongstabodee, Phuwadej Pornaroontham, Nuthapol Pintuyothin, Nuttapol Pootrakulchote, Nutthavich Thouchprasitchai
CO2 capture performance of bifunctional activated bleaching earth (ABE) was investigated at atmospheric pressure. The sorbents were characterized by means of X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Caron-Hydrogen-Nitrogen analysis (CHN), Fourier transform infrared (FT-IR) and thermal gravimetric analysis (TGA). The CO2 capacity was enhanced via basic-modification and monoethanolamine (MEA) loading of the ABE sorbent to obtain a bifunctional surface property. Here, basic-modified calcined ABE with a 30wt...
October 2016: Journal of Environmental Sciences (China)
Yong Hee Kim, Gook Hwa Kim, Min Sun Kim, Sang-Don Jung
A Multi-electrode array (MEA) was fabricated with electrodes consisting of iridium oxide (IrOx) electrochemically deposited on nanoporous gold (NPG) to improve the moderate charge injection limit (ca. 1 mC cm-2) of NPG MEA. IrOx was electrodeposited by performing cyclic voltammetry with an IrOx deposition solution. The IrOx was electrodeposited on Au (EIROF/Au) and on NPG (EIROF/NPG) MEA, and the samples were analyzed in terms of the charge injection limit, charge storage capacity (CSC), and electrochemical impedance...
October 13, 2016: Nano Letters
Silvia Lee, Markus Durstberger, Beate Eichelberger, Christoph W Kopp, Renate Koppensteiner, Simon Panzer, Thomas Gremmel
BACKGROUND: The beneficial effects of ß-blockers on the long-term prognosis of patients with cardiovascular disease may in part be attributable to decreased platelet activation. In this prospective cohort study, we sought to investigate the impact of concomitant ß-blocker therapy on sensitive markers of platelet activation and aggregation. MATERIALS AND METHODS: Monocyte- (MPA) and neutrophil-platelet aggregate (NPA) formation in vivo and in response to the platelet agonist adenosine diphosphate (ADP) were determined by flow cytometry in 258 patients undergoing angioplasty and stenting...
October 13, 2016: European Journal of Clinical Investigation
Didem C Dogan, Seonghun Cho, Sun-Mi Hwang, Young-Min Kim, Hwanuk Guim, Tae-Hyun Yang, Seok-Hee Park, Gu-Gon Park, Sung-Dae Yim
Supportless Pt catalysts have several advantages over conventional carbon-supported Pt catalysts in that they are not susceptible to carbon corrosion. However, the need for high Pt loadings in membrane electrode assemblies (MEAs) to achieve state-of-the-art fuel cell performance has limited their application in proton exchange membrane fuel cells. Herein, we report a new approach to the design of a supportless Pt catalyst in terms of catalyst layer architecture, which is crucial for fuel cell performance as it affects water management and oxygen transport in the catalyst layers...
October 10, 2016: ACS Applied Materials & Interfaces
Peter Psarras, Jiajun He, Jennifer Wilcox
A present challenge in the mitigation of anthropogenic CO2 emissions involves the design of less energy- and water-intensive capture technologies. Sorbent-based capture represents a promising solution, as these materials have negligible water requirements and do not incur the heavy energy penalties associated with solvent regeneration. However, to be considered competitive with traditional technologies (i.e., MEA capture), these sorbents must exhibit a high CO2 loading capacity and high CO2/N2 selectivity. It has been reported that ultramicroporous character and surface nitrogen functionality are of great importance to the enhancement of CO2 capacity and CO2/N2 selectivity...
October 10, 2016: Physical Chemistry Chemical Physics: PCCP
Anne Tscherter, Martina Heidemann, Sonja Kleinlogel, Jürg Streit
Presently there exists no cure for spinal cord injury (SCI). However, transplantation of embryonic tissue into spinal cord (SC) lesions resulted in axon outgrowth across the lesion site and some functional recovery, fostering hope for future stem cell therapies. Although in vivo evidence for functional recovery is given, the exact cellular mechanism of the graft support remains elusive: either the grafted cells provide a permissive environment for the host tissue to regenerate itself or the grafts actually integrate functionally into the host neuronal network reconnecting the separated SC circuits...
2016: Frontiers in Cellular Neuroscience
Paulo R F Rocha, Paul Schlett, Ulrike Kintzel, Volker Mailänder, Lode K J Vandamme, Gunther Zeck, Henrique L Gomes, Fabio Biscarini, Dago M de Leeuw
Microelectrode arrays (MEA) record extracellular local field potentials of cells adhered to the electrodes. A disadvantage is the limited signal-to-noise ratio. The state-of-the-art background noise level is about 10 μVpp. Furthermore, in MEAs low frequency events are filtered out. Here, we quantitatively analyze Au electrode/electrolyte interfaces with impedance spectroscopy and noise measurements. The equivalent circuit is the charge transfer resistance in parallel with a constant phase element that describes the double layer capacitance, in series with a spreading resistance...
October 6, 2016: Scientific Reports
R Matsumura, H Yamamoto, M Niwano, A Hirano-Iwata
Electrical signals of neuronal cells can be recorded non-invasively and with a high degree of temporal resolution using multielectrode arrays (MEAs). However, signals that are recorded with these devices are small, usually 0.01%-0.1% of intracellular recordings. Here, we show that the amplitude of neuronal signals recorded with MEA devices can be amplified by covering neuronal networks with an electrically resistive sheet. The resistive sheet used in this study is a monolayer of glial cells, supportive cells in the brain...
January 11, 2016: Applied Physics Letters
Michael Glas, Janine Viola Bauer, Hermann Eichler, Thomas Volk
Multiple electrode (impedance) aggregometry (MEA) allows reliable monitoring of platelet function in whole blood. The aims of the present study were to implement MEA for analyzing aggregation in platelet concentrates and to correlate results with storage time and blood gas analysis (BGA). We investigated the influence of platelet counts, calcium concentrations and agonists on platelet aggregation. Samples of apheresis concentrates up to an age of 12 days were investigated by MEA and BGA. For ASPI- and TRAPtest MEA was reproducible for a platelet count of 400 per 10(-9 )L and a calcium concentration of 5 mmol L(-1)...
October 5, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Ksenia Blinova, Jayna Stohlman, Jose Vicente, Dulciana Chan, Lars Johannesen, Maria P Hortigon-Vinagre, Victor Zamora, Godfrey Smith, William J Crumb, Li Pang, Beverly Lyn-Cook, James Ross, Mathew Brock, Stacie Chvatal, Daniel Millard, Loriano Galeotti, Norman Stockbridge, David G Strauss
BACKGROUND: Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM) hold promise for assessment of drug-induced arrhythmias and are being considered for use under the Comprehensive in Vitro Proarrhythmia Assay (CiPA). METHODS AND RESULTS: We studied the effects of 26 drugs and 3 drug combinations on two commercially available iPSC-CM types using high-throughput voltage-sensitive dye (VSD) and microelectrode-array (MEA) assays being studied for the Comprehensive in Vitro Proarrhythmia Assessment (CiPA) initiative and compared the results to clinical QT prolongation and torsade de pointes (TdP) risk...
October 3, 2016: Toxicological Sciences: An Official Journal of the Society of Toxicology
Marisela Hernández-González, Orlando Reynoso-Orozco, Miguel Angel Guevara, Diego Radberto Chapa García Abascal, Anders Ågmo
The influence of the duration of vaginocervical stimulation (VCS) on the electroencephalographic activity (EEG) of medial amygdala (MeA) and prefrontal cortex (PFC) in rats during proestrus-estrus (P-E) and diestrus (D) was examined. Using a glass syringe plunger, a constant force of 300 g was exerted against the cervix during 60 s. Relative power (RP) and correlation of three EEG band frequencies were compared between the first and last 30 s intervals of VCS. A higher RP of the 4-7 Hz band and a lower RP of the fast frequencies were observed in the MeA and PFC in P-E females during the first 30 s of VCS as compared to the last 30 s...
September 26, 2016: Brain Research
Noor Mail, Muhammad Yusuf, Nazeeh Alothmany, A Abdulrahman Kinsara, Fahad Abdulkhaliq, Suliman M Ghamdi, Abdelhamid Saoudi
Cone-beam computed tomography CBCT systems are used in radiation therapy for patient alignment and positioning. The CBCT imaging procedure for patient setup adds substantial radiation dose to patient's normal tissue. This study pre-sents a complete procedure for the CBCT dosimetry using the InLight optically-stimulated-luminescence (OSL) nanoDots. We report five dose parameters: the mean slice dose (DMSD); the cone beam dose index (CBDIW); the mean volume dose (DMVD); point-dose profile, D(FOV); and the off-field Dose...
2016: Journal of Applied Clinical Medical Physics
Benjamin J Zwan, Michael P Barnes, Todsaporn Fuangord, Cameron J Stanton, Daryl J O'Connor, Paul J Keall, Peter B Greer
Multileaf collimator (MLC) positions should be precisely and independently mea-sured as a function of gantry angle as part of a comprehensive quality assurance (QA) program for volumetric-modulated arc therapy (VMAT). It is also ideal that such a QA program has the ability to relate MLC positional accuracy to patient-specific dosimetry in order to determine the clinical significance of any detected MLC errors. In this work we propose a method to verify individual MLC trajectories during VMAT deliveries for use as a routine linear accelerator QA tool...
2016: Journal of Applied Clinical Medical Physics
Enzo Wanke, Francesca Gullo, Elena Dossi, Gaetano Valenza, Andrea Becchetti
Astrocytes reuptake synaptically released glutamate with electrogenic transporters (GluT), and buffer the spike-dependent extracellular K(+) ([K(+)]o) excess with background K(+) channels. We studied neuronal spikes and the slower astrocytic signals on reverberating neocortical cultures and organotypic slices from mouse brains. Spike trains and glial responses were simultaneously captured from individual sites of multi-electrode arrays (MEA), by splitting the recorded traces into appropriate filters, and reconstructing the original signal by deconvolution...
September 28, 2016: Journal of Neurophysiology
Felix Hempel, Jessica Ka-Yan Law, Thanh Chien Nguyen, Walid Munief, Xiaoling Lu, Vivek Pachauri, Anna Susloparova, Xuan Thang Vu, Sven Ingebrandt
Electrophysiological biosensors embedded in planar devices represent a state of the art approach to measure and evaluate the electrical activity of biological systems. This measurement method allows for the testing of drugs and their influences on cells or tissues, cytotoxicity, as well as the direct implementation into biological systems in vivo for signal transduction. Multi-electrode arrays (MEAs) with metal or metal-like electrodes on glass substrates are one of the most common, well-established platforms for this purpose...
September 14, 2016: Biosensors & Bioelectronics
Jenne M Westberry, Michael Meredith
Chemosensory stimuli from same species (conspecific) and different species (heterospecific) elicit categorically different immediate-early gene (IEG) response patterns in medial amygdala in male hamsters and mice. All heterospecific stimuli activate anterior medial amygdala (MeA) but only especially salient heterospecific stimuli, such as those from predators activate posterior medial amygdala (MeP). We previously reported that characteristic patterns of response in separate populations of cells in MeA and MeP distinguish between different conspecific stimuli...
September 20, 2016: Chemical Senses
Kiyoshi Takasuna, Keiichi Asakura, Seiichi Araki, Hiroyuki Ando, Katsuyuki Kazusa, Takashi Kitaguchi, Takeshi Kunimatsu, Shinobu Suzuki, Norimasa Miyamoto
Recent increasing evidence suggests that the currently-used platforms in vitro IKr and APD, and/or in vivo QT assays are not fully predictive for TdP, and do not address potential arrhythmia (VT and/or VF) induced by diverse mechanisms of action. In addition, other cardiac safety liabilities such as functional dysfunction of excitation-contraction coupling (contractility) and structural damage (morphological damage to cardiomyocytes) are also major causes of drug attrition, but current in vitro assays do not cover all these liabilities...
September 17, 2016: Journal of Pharmacological and Toxicological Methods
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