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https://www.readbyqxmd.com/read/28518111/using-enzyme-based-biosensors-to-measure-tonic-and-phasic-glutamate-in-alzheimer-s-mouse-models
#1
Holly C Hunsberger, Sharay E Setti, Ryan T Heslin, Jorge E Quintero, Greg A Gerhardt, Miranda N Reed
Neurotransmitter disruption is often a key component of diseases of the central nervous system (CNS), playing a role in the pathology underlying Alzheimer's disease, Parkinson's disease, depression, and anxiety. Traditionally, microdialysis has been the most common (lauded) technique to examine neurotransmitter changes that occur in these disorders. But because microdialysis has the ability to measure slow 1-20 minute changes across large areas of tissue, it has the disadvantage of invasiveness, potentially destroying intrinsic connections within the brain and a slow sampling capability...
May 3, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28509928/highly-stable-nanostructured-membrane-electrode-assembly-based-on-pt-nb2o5-nanobelts-with-reduced-platinum-loading-for-proton-exchange-membrane-fuel-cells
#2
Yachao Zeng, Xiaoqian Guo, Zhiqiang Wang, Jiangtao Geng, Hongjie Zhang, Wei Song, Hongmei Yu, Zhigang Shao, Baolian Yi
Proton exchange membrane fuel cells are promising candidates for the next-generation power sources; however, poor durability and high cost impede their widespread application. To address this dilemma, a nanostructured membrane electrode assembly (MEA) based on Pt/Nb2O5 nanobelts (NBs) was constructed through hydrothermal synthesis and the physical vapour deposition method. Pt/Nb2O5 NBs were directly aligned with Nafion membrane without ionomer as a binder. The prepared catalyst layer is ultrathin and has ultralow Pt loading...
May 16, 2017: Nanoscale
https://www.readbyqxmd.com/read/28499642/efficiency-of-four-currently-used-decontamination-conditionings-in-romania-against-aspergillus-and-candida-strains
#3
D Lorin, R T Cristina, V Teusdea, E Mitrănescu, F Muselin, M Butnariu, G David, E Dumitrescu
INTRODUCTION: Efficacy of four commercial biocidal products (noted A to D), using manufacturers' recommendations, and a contact time of 30minutes, were evaluated in the purpose of standard SR EN1657: 2006 adapted. METHODS: Were used four strains, two as reference: Aspergillus brasiliensis (niger) (ATCC 16404) and Candida albicans (ATCC 10231), and two isolates: Aspergillus flavus and respectively Aspergillus fumigatus. The inoculum plates containing Malt Extract Agar (MEA) were incubated 48h for C...
May 9, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/28499621/an-adaptive-positivity-thresholding-method-for-automated-ki67-hotspot-detection-akhod-in-breast-cancer-biopsies
#4
David Pilutti, Vincenzo Della Mea, Enrico Pegolo, Francesco La Marra, Fulvio Antoniazzi, Carla Di Loreto
The proliferative activity of breast cancer tissue can be estimated using the Ki67 biomarker. The percentage of positivity of such biomarker is correlated with proliferation and consequently with the prognosis of a breast tumor. Ki67 marked tissue samples are analyzed by an experienced pathologist who identifies the most active areas of tumor cell proliferation called hotspots, and estimates the positivity of each case. A method for the Automated Ki67 Hotspot Detection (AKHoD) is presented in this work. The main objective of the AKHoD method is to automatically and efficiently provide the pathologist with suggestions about Ki67 hotspot areas as a decision support...
April 24, 2017: Computerized Medical Imaging and Graphics: the Official Journal of the Computerized Medical Imaging Society
https://www.readbyqxmd.com/read/28493886/narrow-microtunnel-technology-for-the-isolation-and-precise-identification-of-axonal-communication-among-distinct-hippocampal-subregion-networks
#5
Udit Narula, Andres Ruiz, McKinley McQuaide, Thomas B DeMarse, Bruce C Wheeler, Gregory J Brewer
Communication between different sub regions of the hippocampus is fundamental to learning and memory. However accurate knowledge about information transfer between sub regions from access to the activity in individual axons is lacking. MEMS devices with microtunnels connecting two sub networks have begun to approach this problem but the commonly used 10 μm wide tunnels frequently measure signals from multiple axons. To reduce this complexity, we compared polydimethylsiloxane (PDMS) microtunnel devices each with a separate tunnel width of 2...
2017: PloS One
https://www.readbyqxmd.com/read/28492664/size-tunable-highly-sensitive-microelectrode-arrays-enabled-by-polymer-pen-lithography
#6
Xinlei Ma, Fengwang Li, Zhuang Xie, Mianqi Xue, Zijian Zheng, Xueji Zhang
By combining polymer pen lithography (PPL) patterning with in situ polymerization, we report a straightforward and bottom-up approach for bench-top fabrication of microelectrode arrays (MEAs) with well-controlled dimensions. The as-fabricated MEAs can be used to electrodeposit prussian blue in situ and work as a biosensor for H2O2 with a detection limit as low as 5 nM at a sensitivity of 0.7 A cm(-2) M(-1).
May 11, 2017: Soft Matter
https://www.readbyqxmd.com/read/28483965/pharmacokinetics-of-efavirenz-in-2-3-years-old-children-a-high-dose-of-25-mg-kg-per-day
#7
Claire Pressiat, Madeleine Amorissani-Folquet, Caroline Yonaba, Jean-Marc Treluyer, Désiré Lucien Dahourou, François Eboua, Stéphane Blanche, Véronique Mea-Assande, Naïm Bouazza, Frantz Foissac, Karen Malateste, Sylvie Ouedraogo, Gabrielle Lui, Valériane Leroy, Déborah Hirt
Background: The MONOD ANRS 12206 trial was designated to assess simplification of a successful LPV-based antiretroviral treatment in young HIV-infected children using efavirenz (25 mg/kg/day) to preserve the protease inhibitors class before the age of 3. In this sub-study, EFV concentrations were measured to check the consistency of a 25 mg/kg EFV dose and to compare it with the 2016 FDA recommended dose.Methods: Fifty-two children underwent blood sampling for pharmacokinetic study at 6-month and 12-month after switching to EFV...
May 8, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28483438/construction-of-vascularized-pacemaker-tissues-by-seeding-cardiac-progenitor-cells-and-endothelial-progenitor-cells-into-matrigel
#8
Luping Zhang, Xiaotong Li, Xiaolong Yu, Yuquan Li, Aijun Sun, Chao Huang, Feng Xu, Jinping Guo, Yao Sun, Xi Zhang, Xiangqun Yang, Chuansen Zhang
AIMS: Transplantation of a tissue engineered cardiac pacemaker (TECP) may represent a novel therapy for cardiac sinus node dysfunction. We previously reported that cardiac progenitor cells (CPCs) derived from embryonic heart tubes could differentiate into cardiac pacemaking cells after endothelin-1 treatment. We aimed to examine the feasibility of TECP fabricated from CPCs-derived pacemaking cells and vascularization of TECP fabricated from CPCs-derived pacemaking cells and endothelial progenitor cells (EPCs) in vitro and in vivo implantation...
May 5, 2017: Life Sciences
https://www.readbyqxmd.com/read/28470525/assaying-spontaneous-network-activity-and-cellular-viability-using-multi-well-microelectrode-arrays
#9
Jasmine P Brown, Brittany S Lynch, Itaevia M Curry-Chisolm, Timothy J Shafer, Jenna D Strickland
Microelectrode array (MEA) technology is a neurophysiological method that allows for the spontaneous measure of activity in neural cultures and determination of drug and chemical effects thereon. Recent introduction of multi-well MEA (mwMEA) formats have dramatically increased the throughput of this technology, allowing more efficient compound screening. Rapid characterization of compounds for neuroactivity or neurotoxicity hazard evaluation following acute, chronic, or developmental exposures ideally would also consider compound effects on cell health, and to do so in the same well requires a multiplexed approach...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28466637/on-chip-evaluation-of-dna-methylation-with-electrochemical-combined-bisulfite-restriction-analysis-utilizing-a-carbon-film-containing-a-nanocrystalline-structure
#10
Ryoji Kurita, Hiroyuki Yanagisawa, Tomoyuki Kamata, Dai Kato, Osamu Niwa
This paper reports an on-chip electrochemical assessment of the DNA methylation status in genomic DNA on a conductive nanocarbon film electrode realized with combined bisulfite restriction analysis (COBRA). The film electrode consists of sp(2) and sp(3) hybrid bonds and is fabricated with an unbalanced magnetron (UBM) sputtering method. First, we studied the effect of the sp(2)/sp(3) ratio of the UBM nanocarbon film electrode with p-aminophenol, which is a major electro-active product of the labeling enzyme from p-aminophenol phosphate...
May 12, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28458635/analysis-and-modeling-of-subthreshold-neural-multi-electrode-array-data-by-statistical-field-theory
#11
Måns Henningson, Sebastian Illes
Multi-electrode arrays (MEA) are increasingly used to investigate spontaneous neuronal network activity. The recorded signals comprise several distinct components: Apart from artifacts without biological significance, one can distinguish between spikes (action potentials) and subthreshold fluctuations (local fields potentials). Here we aim to develop a theoretical model that allows for a compact and robust characterization of subthreshold fluctuations in terms of a Gaussian statistical field theory in two spatial and one temporal dimension...
2017: Frontiers in Computational Neuroscience
https://www.readbyqxmd.com/read/28456566/assessment-of-acute-and-chronic-toxicity-of-doxorubicin-in-human-induced-pluripotent-stem-cell-derived-cardiomyocytes
#12
Jochem Louisse, Rob C I Wüst, Francesca Pistollato, Taina Palosaari, Manuela Barilari, Peter Macko, Susanne Bremer, Pilar Prieto
The present study assesses acute and chronic toxicity of doxorubicin in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), with the aim to obtain in vitro biomarkers that can be used as readouts to predict in vivo cardiotoxicity. Possible acute toxicity was investigated by assessing effects on the beating rate and the field potential duration (FPD) of doxorubicin-exposed cardiomyocytes by measuring electrical activity using multi-electrode array (MEA) analyses. No effects on the beating rate and FPD were found at concentrations up to 6μM, whereas at 12μM no electrical activity was recorded, indicating that the cardiomyocytes stopped beating...
April 26, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28455403/concerted-divergence-after-gene-duplication-in-polycomb-repressor-complexes
#13
Yichun Qiu, Shao-Lun Liu, Keith L Adams
Duplicated genes are a major contributor to genome evolution and phenotypic novelty. There are multiple possible evolutionary fates of duplicated genes. Here we provide an example of concerted divergence of simultaneously duplicated genes whose products function in the same complex. We studied PRC2 (Polycomb Repressive Complex 2) in Brassicaceae. The VRN-PRC2 complex contains VRN2 and SWN, and both genes were duplicated during a whole genome duplication to generate FIS2 and MEA which function in the Brassicaceae-specific FIS-PRC2 complex that regulates seed development...
April 28, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28454981/neuropharmacological-characterization-of-the-new-psychoactive-substance-methoxetamine
#14
Laura Hondebrink, Emma E J Kasteel, Anke M Tukker, Fiona M J Wijnolts, Anouk H A Verboven, Remco H S Westerink
The use of new psychoactive substances (NPS) is steadily increasing. One commonly used NPS is methoxetamine (MXE), a ketamine analogue. Several adverse effects have been reported following MXE exposure, while only limited data are available on its neuropharmacological modes of action. We investigated the effects of MXE and ketamine on several endpoints using multiple in vitro models. These included rat primary cortical cells, human SH-SY5Y cells, human induced pluripotent stem cell (hiPSC)-derived iCell(®) Neurons, DopaNeurons and astrocyte co-cultures, and human embryonic kidney (HEK293) cells...
April 25, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28453240/virological-response-and-resistances-over-12-months-among-hiv-infected-children-less-than-two-years-receiving-first-line-lopinavir-ritonavir-based-antiretroviral-therapy-in-cote-d-ivoire-and-burkina-faso-the-monod-anrs-12206-cohort
#15
Clarisse Amani-Bosse, Désiré Lucien Dahourou, Karen Malateste, Madeleine Amorissani-Folquet, Malik Coulibaly, Sophie Dattez, Arlette Emieme, Mamadou Barry, Christine Rouzioux, Sylvie N'gbeche, Caroline Yonaba, Marguerite Timité-Konan, Véronique Mea, Sylvie Ouédraogo, Stéphane Blanche, Nicolas Meda, Carole Seguin-Devaux, Valériane Leroy
INTRODUCTION: Lopinavir/ritonavir-based antiretroviral therapy (ART) is recommended for all HIV-infected children less than three years. However, little is known about its field implementation and effectiveness in West Africa. We assessed the 12-month response to lopinavir/ritonavir-based antiretroviral therapy in a cohort of West African children treated before the age of two years. METHODS: HIV-1-infected, ART-naive except for a prevention of mother-to-child transmission (PMTCT), tuberculosis-free, and less than two years of age children with parent's consent were enrolled in a 12-month prospective therapeutic cohort with lopinavir/ritonavir ART and cotrimoxazole prophylaxis in Ouagadougou and Abidjan...
April 25, 2017: Journal of the International AIDS Society
https://www.readbyqxmd.com/read/28447452/single-usage-of-a-kitchen-degreaser-can-alter-indoor-aerosol-composition-for-days
#16
Jaroslav Schwarz, Otakar Makeš, Jakub Ondráček, Michael Cusack, Nicholas Talbot, Petr Vodička, Lucie Kubelová, Vladimír Ždímal
To the best of our knowledge, this study represents the first observation of multiday persistence of an indoor aerosol transformation linked to a kitchen degreaser containing monoethanol amine (MEA). MEA remaining on the cleaned surfaces and on a wiping paper towel in a trash can was able to transform ammonium sulfate and ammonium nitrate into (MEA)2SO4 and (MEA)NO3. This influence persisted for at least 60 h despite a high average ventilation rate. The influence was observed using both offline (filters, impactors, and ion chromatography analysis) and online (compact time-of-flight aerosol mass spectrometer) techniques...
May 10, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28438071/time-to-re-evaluate-art-protocols-in-the-light-of-advances-in-knowledge-about-methylation-and-epigenetics-an-opinion-paper
#17
Yves Menezo, Brian Dale, Kay Elder
DNA methylation is a biochemical process that modifies gene expression without changing the underlying DNA sequence, and this represents the molecular basis for imprinting and epigenetics. Recent reports have revealed alterations in DNA methylation profiles in the placenta of babies born from assisted reproductive technologies (ART). This supports several previous observations that suggested an increase in the prevalence of imprinting diseases following ART treatment, and also fits our observations regarding the metabolism and requirements of early human embryos...
April 25, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28431151/dietary-choline-and-phospholipid-supplementation-enhanced-docosahexaenoic-acid-enrichment-in-egg-yolk-of-laying-hens-fed-a-2-schizochytrium-powder-added-diet
#18
H Wang, H J Zhang, X C Wang, S G Wu, J Wang, L Xu, G H Qi
The aim of this study was to evaluate the effect of dietary phospholipid supplementation on laying hen performance, egg quality, and the fatty acid profile of egg yolks from hens fed a 2% Schizochytrium powder diet. Three-hundred-sixty 28-wk-old Hy-line W-36 laying hens were randomly allocated to one of the 5 dietary treatments, each treatment with 6 replicates of 12 birds each. All diets included 2% Schizochytrium powder (docosahexaenoic acid [DHA], 137.09 mg/g). The control group was not supplemented with any additional phospholipids, whereas the other 4 experimental diets were supplemented with 1,000 mg/kg choline (CHO), 1,000 mg/kg monoethanolamine (MEA), 1,000 mg/kg lysophosphatidylcholine (LPC), or 500 mg/kg LPC + 500 mg/kg MEA (LPC + MEA)...
April 20, 2017: Poultry Science
https://www.readbyqxmd.com/read/28423836/piloting-a-collaborative-web-based-system-for-testing-icd-11
#19
Marc Donada, Nenad Kostanjsek, Vincenzo Della Mea, Can Celik, Robert Jakob
BACKGROUND: The 11<sup>th</sup> revision of the International Classification of Diseases (ICD-11), for the first time in ICD history, deployed web-based collaboration of experts and ICT tools. To ensure that ICD-11 is working well, it needs to be systematically field tested in different settings, across the world. This will be done by means of a number of experiments. In order to support its implementation, a web-based system (ICDfit) has been designed and developed. The present paper illustrates the current prototype of the system and its technical testing...
2017: Studies in Health Technology and Informatics
https://www.readbyqxmd.com/read/28420990/managed-entry-agreements-for-oncology-drugs-lessons-from-the-european-experience-to-inform-the-future
#20
Kim Pauwels, Isabelle Huys, Sabine Vogler, Minne Casteels, Steven Simoens
Objectives: The aim of this study is to conduct an analysis on the regulation and application of managed entry agreements (MEA) for oncology drugs across different European countries. Methods: Literature search and document analysis were performed between September 2015 and June 2016 to collect information on the regulatory framework and practice of MEA in Belgium, The Netherlands, Scotland, England and Wales, Sweden, Italy, Czech Republic and France. An overview of the content and typology of MEA applied for oncology drugs between 2008 and 2015 was generated based on publically available sources and contributions by national health authorities...
2017: Frontiers in Pharmacology
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