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https://www.readbyqxmd.com/read/28087386/androgens-and-androgen-receptor-signaling-contribute-to-ovarian-development-in-the-chicken-embryo
#1
Ryoma Tanaka, Hiroe Izumi, Asato Kuroiwa
Androgens and androgen receptor (AR) signaling play important roles throughout development. In the chicken, AR signaling is involved in reproduction; however, its specific role is unclear. We show that AR signaling is involved in the normal development of the female embryonic gonads. The AR mRNA level was detected in male and female embryonic gonads by quantitative RT-PCR, and its expression was higher in females than in males at all developmental stages examined. In female embryos, the AR localized to nuclei of cells in the left gonad...
January 10, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28087075/cellular-transcriptional-response-to-zirconia-based-implant-materials
#2
Brigitte Altmann, Kerstin Rabel, Ralf J Kohal, Susanne Proksch, Pascal Tomakidi, Erik Adolfsson, Falk Bernsmann, Paola Palmero, Tobias Fürderer, Thorsten Steinberg
OBJECTIVE: To adequately address clinically important issues such as osseointegration and soft tissue integration, we screened for the direct biological cell response by culturing human osteoblasts and gingival fibroblasts on novel zirconia-based dental implant biomaterials and subjecting them to transcriptional analysis. METHODS: Biomaterials used for osteoblasts involved micro-roughened surfaces made of a new type of ceria-stabilized zirconia composite with two different topographies, zirconium dioxide, and yttria-stabilized zirconia (control)...
January 10, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28081379/direct-gingival-fibroblast-osteoblast-transdifferentiation-via-epigenetics
#3
Y Cho, B Kim, H Bae, W Kim, J Baek, K Woo, G Lee, Y Seol, Y Lee, Y Ku, I Rhyu, H Ryoo
Alveolar bone resorption caused by trauma or periodontal diseases has represented a challenge for both dental clinicians and researchers. In this study, we evaluate the osteogenic potential of human gingival fibroblasts (HGFs) through a direct transdifferentiation from HGFs to functional osteoblasts via epigenetic modification and osteogenic signaling with bone morphogenetic protein 2 (BMP2) in vitro and in vivo. HGF treatment with 5-aza-2'-deoxycytidine (5-aza-dC) induced demethylation in the hypermethylated CpG islands of the osteogenic lineage marker genes RUNX2 and ALP, and subsequent BMP2 treatment successfully drove the fibroblasts to the osteoblasts' lineage...
January 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28079305/gallium-enhances-reconstructive-properties-of-a-calcium-phosphate-bone-biomaterial
#4
Ivana Strazic Geljic, Nicolas Melis, Florian Boukhechba, Sébastien Schaub, Charlotte Mellier, Pascal Janvier, Jean-Pierre Laugier, Jean-Michel Bouler, Elise Verron, Jean-Claude Scimeca
Calcium phosphate-based (CaP) biomaterials are commonly used in bone reconstructive surgery to replace the damaged tissue, and can also serve as vectors for local drug delivery. Due to its inhibitory action on osteoclasts, the semi-metallic element gallium (Ga) is used for the systemic treatment of disorders associated with accelerated bone resorption. As it was demonstrated that Ga could be incorporated in the structure of CaP biomaterials, we investigated in the present work the biological properties of Ga-loaded CaP biomaterials...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28078570/micro-rna-expression-in-muscle-and-fiber-morphometry-in-myotonic-dystrophy-type-1
#5
Chiara Fritegotto, Chiara Ferrati, Valentina Pegoraro, Corrado Angelini
We aimed to explore the cellular action of micro-RNAs that are non-coding-RNAs modulating gene expression, whose expression is dysregulated in myotonic dystrophy (DM1). Basic procedure was to measure the levels of muscle-specific myo-miRNAs (miR-1, miR-133a/b, miR-206) in muscle of 12 DM1 patients. Muscle fiber morphometry and a new grading of histopathological severity score were used to compare specific myo-miRNA level and fiber atrophy. We found that the levels of miR-1 and miR-133a/b were significantly decreased, while miR-206 was significantly increased as compared to controls...
January 11, 2017: Neurological Sciences
https://www.readbyqxmd.com/read/28076852/mir-874-3p-is-down-regulated-in-hepatocellular-carcinoma-and-negatively-regulates-pin1-expression
#6
Ka-Wai Leong, Chi-Wai Cheng, Chun-Ming Wong, Irene Oi-Lin Ng, Yok-Lam Kwong, Eric Tse
PIN1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that regulates multiple signaling pathways to control cell fate and is found to be over-expressed in cancers, including hepatocellular carcinoma (HCC). However, the regulation of PIN1 in HCC remains poorly defined. Micro-RNAs (miRNAs) have been reported to play a pivotal role in oncogenesis by targeting the 3'-untranslated region (UTR) of mRNAs encoded by oncogenes and tumour suppressor genes, thereby suppressing the levels of both oncoproteins and tumour suppressors...
January 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28075550/vascularization-in-engineered-tissue-construct-by-assembly-of-cellular-patterned-micro-modules-and-degradable-microspheres
#7
Meiling Zhong, Dan Wei, You Yang, Jing Sun, Xuening Chen, Likun Guo, Qingrong Wei, Yizao Wan, Hongsong Fan, Xingdong Zhang
Tissue engineering aims to generate functional tissue constructs in which proper ECM for cell survival and establishment of vascular network are necessary. A modular approach via the assembly of modules mimicking the complex tissues' micro-architectural features and establishing vascular network represents a promising strategy for fabricating larger and more complex tissue constructs. Herein, as a model for this modular tissue engineering, engineered bone-like constructs were developed by self-assembly of osteon-like modules and fast degradable gelatin microspheres...
January 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28073170/loss-of-hsef-promotes-metastasis-through-up-regulation-of-emt-in-prostate-cancer
#8
S Hori, K Wadhwa, V Pisupati, V Zecchini, A Ramos-Montoya, A Y Warren, D E Neal, V J Gnanapragasam
We have previously reported that the negative regulator Similar Expression to FGF (hSef) is down-regulated in prostate cancer and its loss is associated with clinical metastasis. Here, we explored the mechanistic basis of this finding. We first confirmed our clinical observation by testing hSef manipulation in an in vivo metastasis model. hSef stable expressing cells (PC3M-hSef) or empty vector controls (PC3M-EV) were injected subcutaneously into the lateral thoracic walls of NOD-SCID gamma mice and lungs were harvested at autopsy...
January 10, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28073061/the-significance-of-micrornas-in-the-course-of-rdd
#9
Kinga Bobińska, Janusz Szemraj, Joanna Mossakowska-Wójcik, Piotr Gałecki, Monika Talarowska
BACKGROUND: In recent years, special attention in genetic studies dedicated to the development of various diseases, including mental disorders, has been paid to micro ribonucleic acids (miRNA, microRNA). As an object of our analysis we have selected the miRNAs which - due to the profile of their activity - may be significant in the aetiology and course of recurrent depressive disorders, i.e. miRNA-370, miRNA-411, miRNA-433, miRNA-487b and miRNA-539. METHODS: The examined population included 138 patients suffering from depression and 95 individuals from the control group (CG)...
October 14, 2016: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/28072825/dietary-chromium-restriction-of-pregnant-mice-changes-the-methylation-status-of-hepatic-genes-involved-with-insulin-signaling-in-adult-male-offspring
#10
Qian Zhang, Xiaofang Sun, Xinhua Xiao, Jia Zheng, Ming Li, Miao Yu, Fan Ping, Zhixin Wang, Cuijuan Qi, Tong Wang, Xiaojing Wang
Maternal undernutrition is linked with an elevated risk of diabetes mellitus in offspring regardless of the postnatal dietary status. This is also found in maternal micro-nutrition deficiency, especial chromium which is a key glucose regulator. We investigated whether maternal chromium restriction contributes to the development of diabetes in offspring by affecting DNA methylation status in liver tissue. After being mated with control males, female weanling 8-week-old C57BL mice were fed a control diet (CON, 1...
2017: PloS One
https://www.readbyqxmd.com/read/28071779/diffusive-escape-through-a-narrow-opening-new-insights-into-a-classic-problem
#11
Denis S Grebenkov, Gleb Oshanin
We study the mean first exit time (Tε) of a particle diffusing in a circular or a spherical micro-domain with an impenetrable confining boundary containing a small escape window (EW) of an angular size ε. Focusing on the effects of an energy/entropy barrier at the EW, and of the long-range interactions (LRIs) with the boundary on the diffusive search for the EW, we develop a self-consistent approximation to derive for Tε a general expression, akin to the celebrated Collins-Kimball relation in chemical kinetics and accounting for both rate-controlling factors in an explicit way...
January 10, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28070818/entangling-relation-of-micro-rna-let7-mirna-200-and-mirna-125-with-various-cancers
#12
REVIEW
Nosheen Masood, Azra Yasmin
Involvement of micro RNAs (miRNA) is currently the focus for cancer studies as they effect the post transcriptional expression of different genes. Let-7 family is among the firstly discovered miRNAs that play important role in cell proliferation and dysregulation leading to cell based diseases including cancer. Another family, miRNA-200 prevents transformation of cell to malignant form and tumor formation by interacting with epidermal mesenchymal transition (EMT). Similarly miRNA-125 controls apoptosis and proliferation by affecting multiple genes involved in transcription, immunological defense, resistance against viral and bacterial infections that ultimately leads to cell proliferation, metastasis and finally cancer...
January 9, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/28067984/controlled-release-of-clarithromycin-from-plga-microspheres-enhances-bone-regeneration-in-rabbit-calvaria-defects
#13
Ali Alenezi, Yoshihito Naito, Takayuki Terukina, Widyasri Prananingrum, Yohei Jinno, Tatsuaki Tagami, Tetsuya Ozeki, Silvia Galli, Ryo Jimbo
This study evaluated the sustained release effect of clarithromycin-loaded in PLGA microspheres in a rabbit calvaria defect model. Four bone defects (ø5.0) were created in the calvaria of New Zealand White rabbits (n = 21, n = 7/time point). The defects were randomly designated to four groups. Group 1: No augmentation (sham), Group 2: beta-tricalcium phosphate (β-TCP), Group 3: β-TCP with 0.12 µg clarithromycin, and Group 4: β-TCP with 6.12 µg PLGA microspheres loaded with 0.12 µg Clarithromycin...
January 9, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28067536/history-of-erectile-dysfunction-as-a-predictor-of-poor-physical-performance-after-an-acute-myocardial-infarction
#14
Leonida Compostella, Caterina Compostella, Li Van Stella Truong, Nicola Russo, Tiziana Setzu, Sabino Iliceto, Fabio Bellotto
Background Erectile dysfunction may predict future cardiovascular events and indicate the severity of coronary artery disease in middle-aged men. The aim of this study was to evaluate whether erectile dysfunction (expression of generalized macro- and micro-vascular pathology) could predict reduced effort tolerance in patients after an acute myocardial infarction. Patients and methods One hundred and thirty-nine male patients (60 ± 12 years old), admitted to intensive cardiac rehabilitation 13 days after a complicated acute myocardial infarction, were evaluated for history of erectile dysfunction using the International Index of Erectile Function questionnaire...
January 1, 2017: European Journal of Preventive Cardiology
https://www.readbyqxmd.com/read/28065384/the-effects-of-fat-induced-obesity-on-bone-metabolism-in-rats
#15
Wei Li, Peng Xu, Cuizhe Wang, Xiaodan Ha, Yajuan Gu, Yan Wang, Jun Zhang, Jianxin Xie
OBJECTIVE: The aim of the current study was to investigate the effects of obesity, induced via a high-fat diet, on bone metabolism in rats. METHODS: Two hundred healthy Wistar male rats aged 4 weeks were fed a standard diet and a high-fat diet. At specific time points (week 0, 4, 6, 8, and 10), plasma was collected to determine the levels of glucose and lipid metabolism. Additionally, enzyme-linked immunoassays were performed to determine the plasma levels of adipocyte and bone metabolism factors...
January 3, 2017: Obesity Research & Clinical Practice
https://www.readbyqxmd.com/read/28063212/regulation-of-chondrocyte-functions-by-transient-receptor-potential-cation-channel-v6-in-osteoarthritis
#16
Tengfei Song, Jun Ma, Lei Guo, Peng Yang, Xuhui Zhou, Tianwen Ye
Transient receptor potential vanilloid (TRPV) channels function to maintain the dynamic balance of calcium signaling and calcium metabolism in bones. The goal of this study was to determine the potential role of TRPV6 in regulation of chondrocytes. The level of TRPV6 expression was analyzed by western blot in articular cartilage derived from the knee joints of osteoarthritis (OA) rat models and OA patients. Bone structure and osteoarthritic changes in the knee joints of TRPV6 knockout mice were examined using micro-computed and histological analysis at the age of 6 and 12 months old...
January 7, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28062497/increased-proangiogenic-activity-of-mobilized-cd34-progenitor-cells-of-patients-with-acute-st-segment-elevation-myocardial-infarction-role-of-differential-micro-rna-378-expression
#17
Christian Templin, Julia Volkmann, Maximilian Y Emmert, Pavani Mocharla, Maja Müller, Nicolle Kraenkel, Jelena R Ghadri, Martin Meyer, Beata Styp-Rekowska, Sylvie Briand, Roland Klingenberg, Milosz Jaguszewski, Christian M Matter, Valentin Djonov, Francois Mach, Stephan Windecker, Simon P Hoerstrup, Thomas Thum, Thomas F Lüscher, Ulf Landmesser
OBJECTIVE: Proangiogenic effects of mobilized bone marrow-derived stem/progenitor cells are essential for cardiac repair after myocardial infarction. Micro-RNAs (miRNA/miR) are key regulators of angiogenesis. We investigated the differential regulation of angio-miRs, that is, miRNAs regulating neovascularization, in mobilized CD34(+) progenitor cells obtained from patients with an acute ST-segment-elevation myocardial infarction (STEMI) as compared with those with stable coronary artery disease or healthy subjects...
December 29, 2016: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28062298/ccl3-and-mmp-9-are-induced-by-tl1a-during-death-receptor-3-tnfrsf25-dependent-osteoclast-function-and-systemic-bone-loss
#18
Fraser L Collins, Jessica O Williams, Anja C Bloom, Ravinder K Singh, Lauren Jordan, Michael D Stone, Laura R McCabe, Eddie C Y Wang, Anwen S Williams
Reduced bone density and secondary osteoporosis, resulting in increased risk of fracture, is a significant complicating factor in the inflammatory arthritides. While the exact etiology of systemic bone loss is not fully elucidated, recent insights into the tumor necrosis factor super family (TNFSF) revealed a potential role for death receptor 3 (DR3/TNFRSF25) and one of its ligands, TNF-like protein 1A (TL1A/TNFSF15). The mechanisms by which DR3/TL1A signalling modulates bone loss are unclear. We investigated the effect of DR3/TL1A signalling upon osteoclast-dependent chemokine and MMP production to unravel novel mechanisms whereby this pathway regulates OC formation and OC-dependent bone resorption...
January 3, 2017: Bone
https://www.readbyqxmd.com/read/28060908/experimental-mouse-model-of-lumbar-ligamentum-flavum-hypertrophy
#19
Takeyuki Saito, Kazuya Yokota, Kazu Kobayakawa, Masamitsu Hara, Kensuke Kubota, Katsumi Harimaya, Kenichi Kawaguchi, Mitsumasa Hayashida, Yoshihiro Matsumoto, Toshio Doi, Keiichiro Shiba, Yasuharu Nakashima, Seiji Okada
Lumbar spinal canal stenosis (LSCS) is one of the most common spinal disorders in elderly people, with the number of LSCS patients increasing due to the aging of the population. The ligamentum flavum (LF) is a spinal ligament located in the interior of the vertebral canal, and hypertrophy of the LF, which causes the direct compression of the nerve roots and/or cauda equine, is a major cause of LSCS. Although there have been previous studies on LF hypertrophy, its pathomechanism remains unclear. The purpose of this study is to establish a relevant mouse model of LF hypertrophy and to examine disease-related factors...
2017: PloS One
https://www.readbyqxmd.com/read/28060406/a-simple-protoplast-based-method-for-screening-potent-artificial-mirna-for-maximal-gene-silencing-in-arabidopsis
#20
Nannan Zhang, Dandan Zhang, Jian-Feng Li
The unpredictability of in planta silencing efficiency of artificial micro RNAs (amiRNAs) has greatly limited the application of the amiRNA technology in basic and applied plant research. This unit describes a simple and robust method called the epitope-tagged protein-based amiRNA (ETPamir) screen for identifying the most effective amiRNA for silencing a particular Arabidopsis gene. After selecting three to four amiRNA candidates designed by the WMD3 web server for the target gene, the silencing efficiencies of the amiRNA candidates can be compared by co-expressing individual amiRNAs with the target mRNA encoding an epitope-tagged target protein in Arabidopsis mesophyll protoplasts...
January 5, 2017: Current Protocols in Molecular Biology
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