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https://www.readbyqxmd.com/read/29164558/rapamycin-facilitates-fracture-healing-through-inducing-cell-autophagy-and-suppressing-cell-apoptosis-in-bone-tissues
#1
Z-Y Yin, J Yin, Y-F Huo, J Yu, L-X Sheng, Y-F Dong
OBJECTIVE: To investigate the changes in cell autophagy and the molecular mechanism of rapamycin affecting the fracture healing. MATERIAL AND METHODS: Sprague-Dawley (SD) rats were used to establish the right femoral shaft fracture models, and then underwent immunofluorescence assay to detect the autophagy level in bone tissues. After model establishment, SD rats were divided into two groups, the control group and the rapamycin group (1 mg/kg/d). Respectively, at the 2nd, 4th, and 6th week, rats were randomly selected from each group for X-ray and Micro-computed tomography (Micro-CT) examinations to determine callus growth, immunofluorescence assay to detect the protein expression of light chain 3 II (LC3 II), immunohistochemistry to evaluate the autophagy level through detecting the expression of Beclin1 in rats, Western blotting assay to detect cell apoptosis in tissues, hematoxylin and eosin staining (HE staining) to evaluate the osteoblastic activity through count of osteoblast in bone tissue at the end of fracture, and measure the expression of vascular endothelial growth factors (VEGF)...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29164146/extracorporeal-shock-wave-rebuilt-subchondral-bone-in-vivo-and-activated-wnt5a-ca-2-signaling-in-vitro
#2
Lai Yu, Shuitao Liu, Zhe Zhao, Lin Xia, Haochong Zhang, Jing Lou, Jun Yang, Gengmei Xing, Gengyan Xing
Background: This study aimed to identify the optimal extracorporeal shock wave (ESW) intensity and to investigate its effect on subchondral bone rebuilt in vivo and Wnt5a/Ca(2+) signaling in vitro using an osteoarthritis (OA) rat model and bone marrow mesenchymal stem cells (BMMSCs), respectively. Methods: OA rats treated with (OA + ESW group) or without (OA group) ESW (n = 12/group) were compared with healthy controls (control group, n = 12). Gait patterns and subchondral trabecular bone changes were measured...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29163580/testing-of-auxotrophic-selection-markers-for-use-in-the-moss-physcomitrella-provides-new-insights-into-the-mechanisms-of-targeted-recombination
#3
Mikael Ulfstedt, Guo-Zhen Hu, Monika Johansson, Hans Ronne
The moss Physcomitrella patens is unique among plants in that homologous recombination can be used to knock out genes, just like in yeast. Furthermore, transformed plasmids can be rescued from Physcomitrella back into Escherichia coli, similar to yeast. In the present study, we have tested if a third important tool from yeast molecular genetics, auxotrophic selection markers, can be used in Physcomitrella. Two auxotrophic moss strains were made by knocking out the PpHIS3 gene encoding imidazoleglycerol-phosphate dehydratase, and the PpTRP1 gene encoding phosphoribosylanthranilate isomerase, disrupting the biosynthesis of histidine and tryptophan, respectively...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29163473/treadmill-slope-modulates-inflammation-fiber-type-composition-androgen-and-glucocorticoid-receptors-in-the-skeletal-muscle-of-overtrained-mice
#4
Alisson L da Rocha, Bruno C Pereira, Giovana R Teixeira, Ana P Pinto, Fabiani G Frantz, Lucila L K Elias, Fábio S Lira, José R Pauli, Dennys E Cintra, Eduardo R Ropelle, Leandro P de Moura, Rania A Mekary, Ellen C de Freitas, Adelino S R da Silva
Overtraining (OT) may be defined as an imbalance between excessive training and adequate recovery period. Recently, a downhill running-based overtraining (OTR/down) protocol induced the nonfunctional overreaching state, which is defined as a performance decrement that may be associated with psychological and hormonal disruptions and promoted intramuscular and systemic inflammation. To discriminate the eccentric contraction effects on interleukin 1beta (IL-1β), IL-6, IL-10, IL-15, and SOCS-3, we compared the release of these cytokines in OTR/down with other two OT protocols with the same external load (i...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29163415/combination-susceptibility-testing-of-common-antimicrobials-in-vitro-and-the-effects-of-sub-mic-of-antimicrobials-on-staphylococcus-aureus-biofilm-formation
#5
Bing Yang, Zhixin Lei, Yishuang Zhao, Saeed Ahmed, Chunqun Wang, Shishuo Zhang, Shulin Fu, Jiyue Cao, Yinsheng Qiu
The current study was conducted to evaluate the antibacterial combination efficacies, and whether the sub-inhibitory concentrations (sub-MIC) of antibiotics can influent on the biofilm formation of S. aureus. The minimum inhibitory concentration (MIC) of common antibacterial drugs was determined in vitro against clinical isolates of Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Pasteurella multocida (P. multocida) alone and in combination with each other by using the broth microdilution method and the checkerboard micro-dilution method analyzed with the fractional inhibitory concentration index (FICI), respectively...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163226/adenosine-a2a-receptor-modulates-the-activity-of-globus-pallidus-neurons-in-rats
#6
Hui-Ling Diao, Yan Xue, Xiao-Hua Han, Shuang-Yan Wang, Cui Liu, Wen-Fang Chen, Lei Chen
The globus pallidus is a central nucleus in the basal ganglia motor control circuit. Morphological studies have revealed the expression of adenosine A2A receptors in the globus pallidus. To determine the modulation of adenosine A2A receptors on the activity of pallidal neurons in both normal and parkinsonian rats, in vivo electrophysiological and behavioral tests were performed in the present study. The extracellular single unit recordings showed that micro-pressure administration of adenosine A2A receptor agonist, CGS21680, regulated the pallidal firing activity...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29163188/elevated-levels-of-peripheral-kynurenine-decrease-bone-strength-in-rats-with-chronic-kidney-disease
#7
Bartlomiej Kalaska, Krystyna Pawlak, Tomasz Domaniewski, Ewa Oksztulska-Kolanek, Beata Znorko, Alicja Roszczenko, Joanna Rogalska, Malgorzata M Brzoska, Pawel Lipowicz, Michal Doroszko, Anna Pryczynicz, Dariusz Pawlak
The diagnosis and treatment of bone disorders in patients with chronic kidney disease (CKD) represent a clinical challenge. CKD leads to mineral and bone complications starting early in the course of renal failure. Recently, we have observed the positive relationship between intensified central kynurenine turnover and bone strength in rats with subtotal 5/6 nephrectomy (5/6 Nx)-induced CKD. The aim of the present study was to determine the association between peripheral kynurenine pathway metabolites and bone strength in rats with 5/6 Nx-induced CKD...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29163169/icariin-enhances-bone-repair-in-rabbits-with-bone-infection-during-post-infection-treatment-and-prevents-inhibition-of-osteoblasts-by-vancomycin
#8
Yang Zhang, Lifeng Shen, Zhujun Mao, Nani Wang, Xuping Wang, Xiaowen Huang, Ying Hu, Dan Shou, Chengping Wen
Vancomycin is an effective antibiotic for treatment of bone infection caused by Staphylococcus aureus, however, a high local concentration of vancomycin might induce a delay in bone union. Icariin has been reported to suppress osteoclastogenes and promote osteogenesis. Our study aimed to investigate the effect of icariin on bone repair after anti-infection treatment in vivo and to explore the resisting effect of icariin on rat calvarial osteoblasts (ROBs) inhibited with high doses of vancomycin. Rabbits with bone infection of S...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29163161/inhibition-of-mir-128-3p-by-tongxinluo-protects-human-cardiomyocytes-from-ischemia-reperfusion-injury-via-upregulation-of-p70s6k1-p-p70s6k1
#9
Gui-Hao Chen, Chuan-Sheng Xu, Jie Zhang, Qing Li, He-He Cui, Xiang-Dong Li, Li-Ping Chang, Rui-Jie Tang, Jun-Yan Xu, Xia-Qiu Tian, Pei-Sen Huang, Jun Xu, Chen Jin, Yue-Jin Yang
Background and Aims: Tongxinluo (TXL) is a multifunctional traditional Chinese medicine that has been widely used to treat cardiovascular and cerebrovascular diseases. However, no studies have explored whether TXL can protect human cardiomyocytes (HCMs) from ischemia/reperfusion (I/R) injury. Reperfusion Injury Salvage Kinase (RISK) pathway activation was previously demonstrated to protect the hearts against I/R injury and it is generally activated via Akt or (and) Erk 1/2, and their common downstream protein, ribosomal protein S6 kinase (p70s6k)...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29162724/progesterone-receptor-a-promotes-invasiveness-and-metastasis-of-luminal-breast-cancer-by-suppressing-regulation-of-critical-micro-rnas-by-estrogen
#10
Thomas McFall, Brooke McKnight, Rayna Rosati, Seongho Kim, Yanfang Huang, Nerissa Viola-Villegas, Manohar Ratnam
Distal metastasis of luminal breast cancer is frequent and incurable, yet the metastasis mechanisms are poorly understood. Estrogen, even at postmenopausal concentrations, suppresses invasiveness of luminal breast cancer cells through the estrogen receptor (ER). Invasive tumors overexpress the short progesterone receptor A (PR-A) isoform. Even at postmenopausal concentrations, progesterone activates PR-A, inducing invasiveness by counteracting estrogens effects, particularly when cells are hyper-sensitized to progesterone by PR-A overexpression...
November 21, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29160393/pro-inflammatory-analysis-of-macrophages-in-contact-with-titanium-particles-and-porphyromonas-gingivalis
#11
Cindy Goes Dodo, Luiz Meirelles, Alejandro Aviles-Reyes, Karina Gonzalez Silvério Ruiz, Jacqueline Abranches, Altair Antoninha Del Bel Cury
During insertion of titanium dental implants, particles may shear from the implant to the periimplant region causing osteolysis, and their association with bacteria can exacerbate the inflammatory reaction. However, the association of a high invasive bacterium from the oral cavity, Porphyromonas gingivalis (Pg), and titanium particles remains unknown. This study evaluated pro-inflammatory reaction of human macrophages in contact with micro and nanoparticles of titanium associated with Porphyromonas gingivalis lipopolysaccharide (PgLPS)...
July 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/29160031/maternal-inheritance-of-bdnf-deletion-with-phenotype-of-obesity-and-developmental-delay-in-mother-and-child
#12
Brooke E Harcourt, Denise V R Bullen, Kung-Ting Kao, Daniella Tassoni, Erin J Alexander, Trent Burgess, Susan M White, Matthew A Sabin
Childhood obesity is a significant world health problem. Understanding the genetic and environmental factors contributing to the development of obesity in childhood is important for the rational design of strategies for obesity prevention and treatment. Brain-derived neurotrophic factor (BDNF) plays an important role in the growth and development of the central nervous system, there is also an evidence that BDNF plays a role in regulation of appetite. Disruption of the expression of this gene in a child has been previously reported to result in a phenotype of severe obesity, hyperphagia, impaired cognitive function, and hyperactivity...
November 21, 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/29158836/a-laser-activated-biocompatible-theranostic-nanoagent-for-targeted-multimodal-imaging-and-photothermal-therapy
#13
Liming Deng, Xiaojun Cai, Danli Sheng, Yang Yang, Eric M Strohm, Zhigang Wang, Haitao Ran, Dong Wang, Yuanyi Zheng, Pan Li, Tingting Shang, Yi Ling, Fengjuan Wang, Yang Sun
Multifunctional nanoparticles have been reported for cancer detection and treatment currently. However, the accurate diagnosis and efficient treatment for tumors are still not satisfied. Here we report on the development of targeted phase change multimodal polymeric nanoparticles for the imaging and treatment of HER2-positive breast cancer. METHODS: We evaluated the multimodal imaging capabilities of the prepared nanoparticles in vitro using agar-based phantoms. The targeting performance and cytotoxicity of the nanoparticles were examined in cell culture using SKBR3 (over-expressing HER2) and MDA-MB-231 (HER2 negative) cells...
2017: Theranostics
https://www.readbyqxmd.com/read/29157048/the-drosha-rs10719-t-c-polymorphism-is-associated-with-preeclampsia-susceptibility
#14
Mahnaz Rezaei, Fatemeh Eskandari, Abbas Mohammadpour-Gharehbagh, Batool Teimoori, Minoo Yaghmaei, Mojgan Mokhtari, Saeedeh Salimi
PURPOSE: Drosha is a member of the micro RNA (miRNA) processing machinery that affects miRNA processing. Single-nucleotide polymorphisms (SNPs) in the Drosha gene might affect microRNA processing and the expression of various genes. The aim of this study is to investigate the association between SNPs in the Drosha gene and preeclampsia (PE) in the southeast of Iran. METHODS: Genotyping of Drosha rs10719 and rs6877842 was performed using blood samples from 219 PE women and 205 healthy control subjects by a polymerase chain reaction-restriction fragment length polymorphism method...
November 20, 2017: Clinical and Experimental Hypertension: CHE
https://www.readbyqxmd.com/read/29156519/in-vivo-assessment-of-the-osteo-protective-effects-of-eugenol-in-alveolar-bone-tissues
#15
Hatem M Abuohashish, Dina A Khairy, Maha M Abdelsalam, Ahmed Alsayyah, Mohammed M Ahmed, Salim S Al-Rejaie
Estrogen deficiency following menopausal provokes alveolar bone loss, remodeling and inflammation. Eugenol is a phenolic compound with wide dental applications and anti-inflammatory properties. In the present study, the potential protective role of eugenol against alveolar bone deformities was investigated in an ovariectomized (OVX) rodent model. Two doses of eugenol (2.5 and 5 mg/kg/d) were administered to OVX animals for 12 weeks. In Serum, markers of bone metabolism and pro-inflammatory cytokines were estimated using ELISA...
November 14, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29156228/effect-of-abiotic-and-biotic-stress-factors-analysis-using-machine-learning-methods-in-zebrafish
#16
Rajasekar Gutha, Suresh Yarrappagaari, Lavanya Thopireddy, Kesireddy Sathyavelu Reddy, Rajeswara Reddy Saddala
In order to understand the mechanisms underlying stress responses, meta-analysis of transcriptome is made to identify differentially expressed genes (DEGs) and their biological, molecular and cellular mechanisms in response to stressors. The present study is aimed at identifying the effect of abiotic and biotic stress factors, and it is found that several stress responsive genes are common for both abiotic and biotic stress factors in zebrafish. The meta-analysis of micro-array studies revealed that almost 4...
November 11, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/29156009/discovering-cancer-vulnerabilities-using-high-throughput-micro-rna-screening
#17
Iva Nikolic, Benjamin Elsworth, Eoin Dodson, Sunny Z Wu, Cathryn M Gould, Pieter Mestdagh, Glenn M Marshall, Lisa G Horvath, Kaylene J Simpson, Alexander Swarbrick
Micro-RNAs (miRNAs) are potent regulators of gene expression and cellular phenotype. Each miRNA has the potential to target hundreds of transcripts within the cell thus controlling fundamental cellular processes such as survival and proliferation. Here, we exploit this important feature of miRNA networks to discover vulnerabilities in cancer phenotype, and map miRNA-target relationships across different cancer types. More specifically, we report the results of a functional genomics screen of 1280 miRNA mimics and inhibitors in eight cancer cell lines, and its presentation in a sophisticated interactive data portal...
November 16, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29155742/isolation-of-intact-whole-mouse-mammary-glands-for-analysis-of-extracellular-matrix-expression-and-gland-morphology
#18
Christopher Thompson, Katherine Keck, Abigail Hielscher
The goal of this procedure was to harvest the #4 abdominal mammary glands from female nulliparous mice in order to assess ECM expression and ductal architecture. Here, a small pocket below the skin was created using Mayo scissors, allowing separation of the glands within the subcutaneous tissue from the underlying peritoneum. Visualization of the glands was aided by the use of 3.5x-R surgical micro loupes. The pelt was inverted and pinned back allowing identification of the intact mammary fat pads. Each of the #4 abdominal glands was bluntly dissected by sliding the scalpel blade laterally between the subcutaneous layer and the glands...
October 30, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29152381/selective-deletion-of-the-soluble-colony-stimulating-factor-1-isoform-in-vivo-prevents-estrogen-deficiency-bone-loss-in-mice
#19
Gang-Qing Yao, Nancy Troiano, Christine A Simpson, Karl L Insogna
Neutralizing CSF1 in vivo completely prevents ovariectomy (OVX)-induced bone loss in mice. There are two isoforms of CSF1, soluble (sCSF1), and membrane-bound (mCSF1), but their individual biological functions are unclear. It had been previously reported that mCSF1 knockout (K/O) and wild type (Wt) female mice experience the same degree of bone loss following OVX. In Wt mice the expression of sCSF1 was elevated fourfold in skeletal tissue following OVX while expression of mCSF1 was unchanged. To examine the role of sCSF1 in OVX-induced bone loss, mice were engineered in which sCSF1 was not expressed but expression of mCSF1 was unaffected (sCSF1 K/O)...
2017: Bone Research
https://www.readbyqxmd.com/read/29152133/prognostic-significance-of-xrcc4-expression-in-hepatocellular-carcinoma
#20
Jun Lu, Xing-Zhizi Wang, Tian-Qi Zhang, Xiao-Ying Huang, Jin-Guang Yao, Chao Wang, Zhong-Hong Wei, Yun Ma, Xue-Min Wu, Chun-Ying Luo, Qiang Xia, Xi-Dai Long
Background: Our previous investigations have shown that the variants of X-ray repair complementing 4 (XRCC4) may be involved in hepatocellular carcinoma (hepatocarcinoma) tumorigenesis. This study aimed to investigate the possible prognostic significance of XRCC4 expression for hepatocarcinoma patients and possible value for the selection of transarterial chemoembolization (TACE) treatment. Materials and Methods: We conducted a hospital-based retrospective analysis (including 421 hepatocarcinoma cases) to analyze the effects of XRCC4 on hepatocarcinoma prognosis and TACE...
October 20, 2017: Oncotarget
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