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Theresa L Barke, Jeffery A Goldstein, Alexandra C Sundermann, Arun P Reddy, Jodell E Linder, Hernan Correa, Digna R Velez-Edwards, David M Aronoff
PROBLEM: GDM has been associated with disturbances in iron homeostasis and exaggerated immune activation. We sought to investigate the extent to which placental iron storage and macrophage accumulations were altered in GDM. METHOD OF STUDY: We conducted a retrospective, case-control study of archived placental tissues obtained from 22 pregnancies complicated by GDM and 22 unaffected controls. Controls were matched to cases based on maternal age, gestational age at birth, and method of delivery...
July 9, 2018: American Journal of Reproductive Immunology: AJRI
Lauren Richardson, Ramkumar Menon
Amnion epithelial cell (AEC) shedding causes microfractures in human placental membranes during gestation. However, microfractures are healed to maintain membrane integrity. To better understand the cellular mechanisms of healing and tissue remodeling, scratch assays were performed using primary AECs derived from normal term not in labor membranes. AECs were grown under different conditions: i) normal cultures (control), ii) oxidative stress (OS) induction by cigarette smoke extract (CSE), iii) co-treatment of CSE and antioxidant N-acetyl-l-cysteine, and iv) treatment with amniotic fluid (AF)...
July 5, 2018: American Journal of Pathology
Shuai Zhou, Chengjia Sun, Shaohui Huang, Ximing Wu, Yan Zhao, Chunling Pan, Hongyan Wang, Junchao Liu, Qian Li, Yurong Kou
Objectives: Lots of bioactive materials have been additionally applied for the treatment of periodontal intrabony defect. However, there is dearth of studies to systematically evaluate the supplementary role of them in periodontal regeneration. The goal of this meta-analysis is to evaluate the adjunctive effects of bioactive materials such as platelet-rich plasma (PRP), platelet-rich fibrin (PRF), enamel matrix derivative (EMD), and amnion membrane (AM) on the outcomes of bone grafting treatment for periodontal intrabony defects...
2018: BioMed Research International
Matthew A Benton
In Drosophila melanogaster, the germband forms directly on the egg surface and solely consists of embryonic tissue. In contrast, most insect embryos undergo a complicated set of tissue rearrangements to generate a condensed, multilayered germband. The ventral side of the germband is embryonic, while the dorsal side is thought to be an extraembryonic tissue called the amnion. While this tissue organisation has been accepted for decades and has been widely reported in insects, its accuracy has not been directly tested in any species...
July 3, 2018: PLoS Biology
Jaspreet Kaur, Shalu Chandna Bathla
Context: Platelet-rich fibrin (PRF), platelet concentrate composed of cytokines and structural glycoproteins trapped within polymerized fibrin meshwork, has the potential to accelerate healing of periodontal tissues. Amnion membrane has also attracted a lot of attention in periodontal regeneration due to the presence of variety of growth factors. Aims: The aim of the study is to evaluate and compare the regenerative potential of autologous PRF with and without amnion membrane in the treatment of Grade II furcation defects...
May 2018: Journal of Indian Society of Periodontology
Richard J Sanders, Stephen J Annest
A patient with neurogenic thoracic outlet syndrome was initially treated with scalenectomy, first rib resection, and wrapping of the brachial plexus (BP) with amnion membrane (AM) to prevent postoperative adhesions. Twelve months later, at reoperation for recurrent symptoms, the AM was observed to be intact. The BP had no scar tissue around it. Recurrence was due to scarring around the nerve roots superior to the portion of the plexus that had been wrapped with AM. It was concluded that the AM had successfully protected the portion of the BP that had been wrapped...
June 2018: Journal of Vascular Surgery Cases and Innovative Techniques
Jin Jin, Lauren Richardson, Samantha Sheller-Miller, Nanbert Zhong, Ramkumar Menon
OBJECTIVE: This study tested the mechanism of the oxidative stress (OS)-induced senescence pathway at the feto-maternal interface cells. METHODS: Primary amnion mesenchymal cells (AMCs), chorion and decidual cells isolated from the placental membranes of women at normal term (not in labor) were exposed to OS-inducing cigarette smoke extract (CSE) for 48 h. Reactive oxygen species (ROS) was measured using 2'7'-dichlorodihydrofluorescein. Western blot analysis determined phosphorylated (P) p38MAPK and p53 expression...
July 2018: Placenta
Victoria Schlanser, Andrew Dennis, Katarina Ivkovic, Kimberly Joseph, Matthew Kaminsky, Thomas Messer, Stathis Poulakidas, Frederic Starr, Faran Bokhari
Reconstruction of skin and soft tissue wounds can pose a unique surgical challenge. This is especially true for cases of exposed bone and tendon where soft tissue loss is extensive and opportunities for tissue advancement or rotation are limited. A clinical case is presented describing an experience with dehydrated human amnion/chorion membrane (dHACM, EpiFix®/AmnioFix®, MiMedx Group, Marietta, GA) graft to obtain granulation over an open fracture with desiccated bone. The 22-year-old female trauma patient presented with high-grade bilateral lower extremity soft tissue loss after being run over and dragged by a semitruck...
December 5, 2017: Journal of Burn Care & Research: Official Publication of the American Burn Association
Thanh G Phan, Henry Ma, Rebecca Lim, Christopher G Sobey, Euan M Wallace
Background: There is increasing interest in stem cell therapy as another treatment modality in stroke, particularly for patients who are unable to receive endovascular clot retrieval or thrombolysis therapies, or for whom standard treatment has failed. We have recently shown that human amniotic epithelial cells (hAECs) are effective in reducing infarct volume in different animal models of ischemic stroke, including in non-human primates. hAEC therapy attenuated infarct growth and/or promoted functional recovery, even when administered 1-3 days after the onset of stroke...
2018: Frontiers in Neurology
Yannick Robert Devaud, Silvia Züger, Roland Zimmermann, Martin Ehrbar, Nicole Ochsenbein-Kölble
INTRODUCTION: The benefits of endoscopic fetal surgery are deteriorated by the high risk of iatrogenic preterm prelabor rupture of fetal membranes (iPPROM). While previous studies have reported good sealing candidates to prevent membrane rupture, the delivery of these materials to the location of membrane puncture remains unsolved. MATERIALS AND METHODS: We describe an approach to apply sealing materials onto the amnion through the fetoscopy port. We developed a device composed of an umbrella-shaped polyester coated nitinol mesh and an applicator...
June 19, 2018: Fetal Diagnosis and Therapy
Shinsuke Otagiri, Shunsuke Ohnishi, Arisa Miura, Hiroshi Hayashi, Izumi Kumagai, Yoichi M Ito, Takehiko Katsurada, Shiro Nakamura, Rika Okamoto, Kenichi Yamahara, Kyu Yong Cho, Toshiyuki Isoe, Norihiro Sato, Naoya Sakamoto
Introduction: The medical treatment options for patients with Crohn's disease (CD) are limited and patients resistant to those therapies are left requiring surgical operations that usually only achieve some symptomatic relief. Mesenchymal stem cells (MSC) have been shown to be effective for the treatment of CD, and we have demonstrated in animal experiments that human amnion-derived MSCs (AMSC) are a potential new therapeutic strategy. Therefore, we designed this study to investigate the safety and efficacy of AMSCs in patients with treatment-resistant CD...
2018: BMJ Open Gastroenterology
Srinivasan Sivasubramanian, Sambasivam Mohana, Paulraj Maheswari, Victor Victoria, Ramar Thangam, Jayashri Mahalingam, Gayathri Chandrasekar-Janebjer, Vincent Savariar, Balaraman Madhan, Palani Gunasekaran, Satish S Kitambi
Background: Deformities and neuropathic chronic ulcers are the common features associated with leprosy-cured individuals that impact their quality of life and impair rehabilitation efforts. The challenging aspects for treatment of chronic wounds are the factors that inhibit healing. We reasoned that limited success of various therapeutic interventions could be due to the fact that leprosy-cured individual's physiology gets acclimatized to having a chronic wound that any therapeutic intervention is counterbalanced to maintain status quo at the wound site...
April 2018: Journal of Global Infectious Diseases
István Gera
Data from epidemiological and clinical studies published in the past two decades indicate certain association between periodontal disease and increased risk for preterm birth or low birth weight. Although the strength of those observed associations is weak, periodontitis today is considered as one of the potentially modifiable risk factors for adverse pregnancy outcomes. The aims of the publication are to summarize the epidemiological and clinical evidence for the impact of periodontal disease on adverse pregnancy outcomes and to make an attempt to overview the potential biological mechanism behind this association...
June 2018: Orvosi Hetilap
Juliane Ertl, Melanie Pichlsberger, Alexandru-Cristian Tuca, Paul Wurzer, Jakob Fuchs, Stefan H Geyer, Barbara Maurer-Gesek, Wolfgang J Weninger, Dagmar Pfeiffer, Vladimir Bubalo, Daryousch Parvizi, Lars-Peter Kamolz, Ingrid Lang
OBJECTIVE: Mesenchymal stem/stromal cells derived from human term placentas (PMSCs) are novel therapeutic agents and more topical than ever. Here we evaluated the effects of three types of PMSCs on wound healing in an in vivo mouse model: Amnion-derived MSCs (AMSCs), blood vessel-derived MSCs (BV-MSCs) from the chorionic plate and Wharton's jelly-derived MSCs (WJ-MSCs) from the umbilical cord. METHODS: We topically applied PMSCs onto skin wounds in mice using the dermal substitute Matriderm® as carrier and evaluated wound healing parameters...
May 2018: Placenta
Nigel Farrow, Patricia Cmielewski, Martin Donnelley, Nathan Rout-Pitt, Yuben Moodley, Ivan Bertoncello, David Parsons
BACKGROUND: Airway disease is a primary cause of morbidity and early mortality for patients with cystic fibrosis (CF). Cell transplantation therapy has proven successful for treating immune disorders and may have the potential to correct the airway disease phenotype associated with CF. Since in vivo cell delivery into unconditioned mouse airways leads to inefficient engraftment, we hypothesised that disrupting the epithelial cell layer using the agent polidocanol (PDOC) would facilitate effective transplantation of cultured stem cells in mouse nasal airways...
June 13, 2018: Stem Cell Research & Therapy
Lauren Richardson, Christopher Luke Dixon, Leopoldo Aguilera-Aguirre, Ramkumar Menon
Term and preterm parturition are associated with oxidative stress (OS)-induced p38 mitogen-activated protein kinase (MAPK)-mediated fetal tissue (amniochorion) senescence. p38MAPK activation is a complex cell and stimulant-dependent process. Two independent pathways of OS-induced p38MAPK activation were investigated in amnion epithelial cells (AECs) in response to cigarette smoke extract (CSE: a validated OS inducer in fetal cells: 1) The OS-mediated oxidation of apoptosis-signaling kinase (ASK)-1 bound Thioredoxin (Trx[SH]2 dissociates this complex, creating free and activated ASK1-signalosome and 2) transforming growth factor mediated activation of (TGF)-beta-activated kinase (TAK)1 and TGF-beta-activated kinase 1-binding protein (TAB)1...
June 11, 2018: Biology of Reproduction
Mariya P Dobreva, Vanesa Abon Escalona, Kirstie A Lawson, Marina N Sanchez, Ljuba C Ponomarev, Paulo N G Pereira, Agata Stryjewska, Nathan Criem, Danny Huylebroeck, Susana M Chuva de Sousa Lopes, Stein Aerts, An Zwijsen
Upon gastrulation, the mammalian conceptus transforms rapidly from a simple bilayer into a multilayered embryo enveloped by its extra-embryonic membranes. Impaired development of the amnion, the innermost membrane, causes major malformations. To clarify the origin of the mouse amnion, we used single-cell labelling and clonal analysis. We identified four clone types with distinct clonal growth patterns in amniotic ectoderm. Two main types have progenitors in extreme proximal-anterior epiblast. Early descendants initiate and expand amniotic ectoderm posteriorly, while descendants of cells remaining anteriorly later expand amniotic ectoderm from its anterior side...
July 2, 2018: Development
Mohd Rehan, Manish Khatri, Mansi Bansal, Komal Puri, Ashish Kumar
Background: An amnion membrane is a placenta-derived tissue that consists of numerous growth factors, proteins, and stem cell reserves which help in accelerated wound healing and regeneration. Platelet-rich fibrin (PRF) also releases growth factors after activation from the platelets and gets trapped within fibrin matrix which has been shown to stimulate the mitogenic response in the periosteum for bone repair and regeneration during normal wound healing. This preliminary, controlled, randomized clinical trial with an 18-month follow-up was aimed to evaluate the effectiveness of coronally advanced flap (CAF) with either PRF membrane or bioresorbable amniotic membrane (AM) in treatment of localized gingival recession defects...
April 2018: Contemporary Clinical Dentistry
Thorsten Braun, Katharina Weizsäcker, Mustafa Zelal Muallem, Janina Tillinger, Larry Hinkson, Frederic Chantraine, Wolfgang Henrich
The number of pregnant women with abnormally invasive placenta (AIP) including clinical relevant placenta increta and percreta has markedly increased with a reported incidence of as high as one in 731, By 2020 in the United States, there will be an estimated 4504 new cases of AIP and 130 AIP-associated maternal deaths annually. The preoperative diagnosis and operative management of AIP is challenging. In a planned cesarean delivery, a vertical lower abdominal skin incision is widely used in order to have enough space to perform a hysterotomy above the cranial edge of the placenta to avoid significant fetal and/or maternal hemorrhage...
June 5, 2018: Journal of Perinatal Medicine
Brittany M Woodall, Nicholas Elena, Joseph T Gamboa, Edward C Shin, Neil Pathare, Patrick J McGahan, James L Chen
Anterior cruciate ligament (ACL) ruptures are common and unfortunate injuries for many athletes. The standard therapy for ACL rupture is ACL reconstruction with either autograft, harvested from hamstring or patellar tendon, or allograft tendon from a tissue donor. Advances in tissue engineering have produced interventions to augment the healing process and may have applications when it comes to ACL reconstruction. In this Technical Note and accompanying video, we describe a simple technique to implant an amnion matrix graft with a tendon graft during ACL reconstruction...
April 2018: Arthroscopy Techniques
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