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https://www.readbyqxmd.com/read/28333279/inhibition-of-pim1-kinase-attenuates-inflammation-induced-pro-labour-mediators-in-human-fetal-membranes-in-vitro
#1
Ratana Lim, Gillian Barker, Martha Lappas
STUDY QUESTION: Does proviral integration site for Moloney murine leukemic virus (PIM)1 kinase play a role in regulating the inflammatory processes of human labour and delivery? SUMMARY ANSWER: PIM1 kinase plays a critical role in fetal membranes in regulating pro-inflammatory and pro-labour mediators. WHAT IS KNOWN ALREADY: Infection and inflammation have strong causal links to preterm delivery by stimulating pro-inflammatory cytokines and collagen degrading enzymes, which can lead to rupture of membranes...
March 9, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28323983/autophagic-degradation-of-collagen-1a1-by-cortisol-in-human-amnion-fibroblasts
#2
Yabing Mi, Wangsheng Wang, Chuyue Zhang, Chao Liu, Jiangwen Lu, Wenjiao Li, Rujuan Zuo, Leslie Myatt, Kang Sun
Rupture of fetal membranes can initiate parturition at both term and preterm. Collagen is the crucial factor determining the tensile strength of the membranes. Toward the end of gestation, a feed-forward regeneration of cortisol via 11β-hydroxysteroid dehydrogenase 1 exists in fetal membranes. It remains undetermined whether cortisol contributes to collagen reduction in fetal membranes. Here we have examined whether cortisol accumulation is a causative factor for collagen reduction in human amnion fibroblasts, the major source of collagens in the membranes...
January 17, 2017: Endocrinology
https://www.readbyqxmd.com/read/28316944/human-amnion-membrane-potential-applications-in-oral-and-periodontal-field
#3
REVIEW
Ranjana Mohan, Aashima Bajaj, Mohan Gundappa
Human amniotic membrane (HAM) is derived from the fetal membranes which consist of the inner amniotic membrane made of single layer of amnion cells fixed to collagen-rich mesenchyme attached to chorion. HAM has low immunogenicity, anti-inflammatory properties and their cells can be isolated without the sacrifice of human embryos. Amniotic membrane has biological properties which are important for the experimental and clinical applications in managing patients of various medical specialties. Abundant, natural and wonderful biomembrane not only protects the foetus but also has various clinical applications in the field of dermatology, ophthalmology, ENT surgery, orthopedics and dental surgery...
January 2017: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/28295207/human-gestation-associated-tissues-express-functional-cytosolic-nucleic-acid-sensing-pattern-recognition-receptors
#4
Aled H Bryant, Georgina E Menzies, Louis M Scott, Samantha Spencer-Harty, Lleucu B Davies, Rachel A Smith, Ruth H Jones, Catherine A Thornton
The role of viral infections in adverse pregnancy outcomes has gained interest in recent years. Innate immune pattern recognition receptors (PRRs) and their signalling pathways that yield a cytokine output in response to pathogenic stimuli have been postulated to link infection at the maternal-fetal interface and adverse pregnancy outcomes. The objective of this study was to investigate the expression and functional response of nucleic acid ligand responsive Toll-like receptors (TLR3, 7, 8 and 9), and retinoic acid-inducible gene 1 (RIG-I)-like receptors (RIG-I, MDA5 and LGP2) in human term gestation-associated tissues (placenta, choriodecidua and amnion) using an explant model...
March 13, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28292466/generation-of-trophoblast-like-cells-from-the-amnion-in%C3%A2-vitro-a-novel-cellular-model-for-trophoblast-development
#5
Yanxing Wei, Xiaohua Zhou, Wenhao Huang, Ping Long, Lu Xiao, Tian Zhang, Mei Zhong, Guangjin Pan, Yanlin Ma, Yanhong Yu
Despite the high incidence of trophoblast-related diseases, the molecular mechanism of inadequate early trophoblast development is still unclear due to the lack of an appropriate cellular model in vitro. In the present study, we reprogrammed the amniotic cells to be induced pluripotent stem cells (iPSCs) via a non-virus and non-integrated method and subsequently differentiated them into trophoblast-like cells by a modified BMP4 strategy in E6 medium. Compared with the previously studied trophoblast-like cells from ESCs, the iPSCs derived trophoblast-like cells behave similarly in terms of gene expression profiles and biofunctions...
March 2017: Placenta
https://www.readbyqxmd.com/read/28291503/regenerative-and-antibacterial-properties-of-acellular-fish-skin-grafts-and-human-amnion-chorion-membrane-implications-for-tissue-preservation-in-combat-casualty-care
#6
Skuli Magnusson, Baldur Tumi Baldursson, Hilmar Kjartansson, Ottar Rolfsson, Gudmundur Fertram Sigurjonsson
BACKGROUND: Improvised explosive devices and new directed energy weapons are changing warfare injuries from penetrating wounds to large surface area thermal and blast injuries. Acellular fish skin is used for tissue repair and during manufacturing subjected to gentle processing compared to biologic materials derived from mammals. This is due to the absence of viral and prion disease transmission risk, preserving natural structure and composition of the fish skin graft. OBJECTIVES: The aim of this study was to assess properties of acellular fish skin relevant for severe battlefield injuries and to compare those properties with those of dehydrated human amnion/chorion membrane...
March 2017: Military Medicine
https://www.readbyqxmd.com/read/28284933/synthesis-of-a-smart-ph-responsive-magnetic-nanocomposite-as-high-loading-carrier-of-pharmaceutical-agents
#7
Razieh Berah, Mohsen Ghorbani, Ali Akbar Moghadamnia
To create facile external controlled drug delivery system, a magnetic porous carrier based on Tin oxide nanoparticles was synthesized by an inexpensive and versatile hydrothermal strategy and used for in-vitro process. Magnetic nanocomposites were qualified by Fourier Transform Infrared (FTIR), Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), Vibrational Sample Magnetometer (VSM) and Transmission Electron Microscopy (TEM). Results showed that nanoparticles were synthesized successfully with good dispersion of magnetic nanoparticles in cavity, uniform particle size distribution with average size of 65nm and high magnetization of 33...
March 8, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28259958/comparison-of-the-proliferation-migration-and-angiogenic-properties-of-human-amniotic-epithelial-and-mesenchymal-stem-cells-and-their-effects-on-endothelial-cells
#8
Qianqian Wu, Tao Fang, Hongxin Lang, Min Chen, Ping Shi, Guoxian Qi, Xining Pang
In vivo studies have shown that amnion-produced growth factors participate in many diseases that involve angiogenesis, re-epithelialization and immunomodulation. Although human amniotic epithelial cells (hAECs) and human amniotic mesenchymal stem cells (hAMSCs) can be obtained from amniotic membranes, there is little information regarding their biological differences. The aim of the present study was to isolate and characterize cells from human amnions, to investigate the biological potential and behavior of these cells on the function of endothelial cells in vivo and in vitro and to examine variations in the expression profile of growth factors in different human amnion-derived cell types...
February 20, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28255007/the-human-chorion-contains-definitive-hematopoietic-stem-cells-from-the-15-th-week-of-gestation
#9
Marcus O Muench, Mirhan Kapidzic, Matthew Gormley, Alan G Gutierrez, Kathryn L Ponder, Marina E Fomin, Ashley I Beyer, Haley Stolp, Zhongxia Qi, Susan J Fisher, Alicia Bárcena
We examined the contribution of the fetal membranes, amnion and chorion, to human embryonic and fetal hematopoiesis. A population of cells displaying a hematopoietic progenitor phenotype (CD34(++/+)CD45(low) cells) of fetal origin was present in the chorion at all gestational ages, associated with stromal cells or near blood vessels, but was absent in the amnion. Prior to 15 weeks of gestation, they lacked hematopoietic in vivo engraftment potential. Differences in the chemokine receptor and β1 integrin expression profiles of progenitors between the 1(st) and 2(nd) trimesters suggest that these cells had gestationally-regulated responses to homing signals and/or adhesion mechanisms that influenced their ability to colonize the stem cell niche...
March 2, 2017: Development
https://www.readbyqxmd.com/read/28243917/conditions-for-collection-of-placental-tissue-samples-for-culturing-of-multipotent-mesenchymal-stromal-cells
#10
N V Nizyaeva, M N Nagovitsyna, G V Kulikova, U N Tumanova, R A Poltavtseva, I A Fedorova, M N Yushina, S V Pavlovich, A I Shchyogolev
Placentas from women aged 25-32 years with normal course of gestation were studied. It is essential to stick to certain methodological approaches for preparing viable multipotent mesenchymal stromal cell culture and to carry out morphological (macro and micro) evaluation of the chorionic villi, umbilical cords, and placentas. At stage I of the study, patients' histories, labor course, and examinations of the newborns should be analyzed to exclude women with genital and extragenital diseases. At stage II, it is essential to stick to special regulations and methods for collection of specimens of the cord, amnion, and placental tissue proper...
February 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28241859/human-amnion-epithelial-cells-rescue-cell-death-via-immunomodulation-of-microglia-in-a-mouse-model-of-perinatal-brain-injury
#11
Bryan Leaw, Dandan Zhu, Jean Tan, Ruth Muljadi, Mohamed I Saad, Joanne C Mockler, Euan M Wallace, Rebecca Lim, Mary Tolcos
BACKGROUND: Human amnion epithelial cells (hAECs) are clonogenic and have been proposed to reduce inflammatory-induced tissue injury. Perturbation of the immune response is implicated in the pathogenesis of perinatal brain injury; modulating this response could thus be a novel therapy for treating or preventing such injury. The immunomodulatory properties of hAECs have been shown in other animal models, but a detailed investigation of the effects on brain immune cells following injury has not been undertaken...
February 28, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28224047/identification-of-extracellular-matrix-components-and-biological-factors-in-micronized-dehydrated-human-amnion-chorion-membrane
#12
Jennifer Lei, Lauren B Priddy, Jeremy J Lim, Michelle Massee, Thomas J Koob
Objective: The use of bioactive extracellular matrix (ECM) grafts such as amniotic membranes is an attractive treatment option for enhancing wound repair. In this study, the concentrations, activity, and distribution of matrix components, growth factors, proteases, and inhibitors were evaluated in PURION(®) Processed, micronized, dehydrated human amnion/chorion membrane (dHACM; MiMedx Group, Inc.). Approach: ECM components in dHACM tissue were assessed by using immunohistochemical staining, and growth factors, cytokines, proteases, and inhibitors were quantified by using single and multiplex ELISAs...
February 1, 2017: Advances in Wound Care
https://www.readbyqxmd.com/read/28221829/the-evolutionary-economics-of-embryonic-sac-fluids-in-squamate-reptiles
#13
Xavier Bonnet, Guy Naulleau, Richard Shine
The parchment-shelled eggs of squamate reptiles take up substantial water from the nest environment, enabling the conversion of yolk into neonatal tissue and buffering the embryo against the possibility of subsequent dry weather. During development, increasing amounts of water are stored in the embryonic sacs (i.e., membranes around the embryo: amnion, allantois, and chorion). The evolution of viviparity (prolonged uterine retention of developing embryos) means that embryonic-sac fluid storage now imposes a cost (increased maternal burdening), confers less benefit (because the mother buffers fetal water balance), and introduces a potential conflict among uterine siblings (for access to finite water supplies)...
March 2017: American Naturalist
https://www.readbyqxmd.com/read/28219475/effects-of-in-ovo-feeding-of-creatine-pyruvate-on-the-hatchability-growth-performance-and-energy-status-in-embryos-and-broiler-chickens
#14
M M Zhao, T Gao, L Zhang, J L Li, P A Lv, L L Yu, F Gao, G H Zhou
The effects of in ovo feeding (IOF) of creatine pyruvate (CrPyr) on the hatchability, growth performance and energy status of embryos and broilers (Arbor Acres) were investigated. Five treatments were arranged as non-injected treatment (Control), 0.6 ml physiological saline (0.75%) injected treatment (Saline), and IOF treatments injected with 0.6 ml physiological saline (0.75%) containing 3, 6 or 12 mg CrPyr (CrPyr3, CrPyr6 or CrPyr12) into the amnion per fertile egg on day 17.5 of incubation. After hatching, 80 male chicks from each treatment with similar weight close to the average BW of their pooled group were selected and randomly assigned into eight replicates of 10 chicks each...
February 21, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28196167/volumetric-assessment-of-regenerative-efficacy-of-demineralized-freeze-dried-bone-allograft-with-or-without-amnion-membrane-in-grade-ii-furcation-defects-a-cone-beam-computed-tomography-study
#15
Natasha G Pajnigara, Abhay P Kolte, Rajashri A Kolte, Nilufer G Pajnigara
Volumetric changes were evaluated to assess the regenerative efficacy of demineralized freeze-dried bone allograft (DFDBA) with and without amnion membrane (AM) in Grade II furcation defects both clinically and radiographically using cone beam computed tomography (CBCT). Sites were randomly assigned to Group I (DFDBA) and Group II (DFDBA + AM). Clinical parameters such as probing pocket depth, clinical attachment level, gingival recession, and horizontal probing depth were assessed at baseline, 3 months, and 6 months...
March 2017: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/28188254/differential-effects-of-oxytocin-receptor-antagonists-atosiban-and-nolasiban-on-ot-mediated-signalling-in-human-amnion-and-myometrium
#16
Sung Hye Kim, Oliver Pohl, Andre Chollet, Jean-Pierre Gotteland, Adam D J Fairhurst, Phillip R Bennett, Vasso Terzidou
One of the most established roles of oxytocin (OT) is in inducing uterine contractions and labour. Apart from contractions, our recent study have shown that OT can also activate pro-inflammatory pathways in both human myometrial and amnion cells which suggests the pro-inflammatory role of OT should be taken into account when developing tocolytics targeting the OT/OT receptor (OT/OTR) system. The OTR antagonist, Atosiban, is currently used therapeutically for the treatment of PTL. We have previously shown that Atosiban fails to inhibit the pro-inflammatory effects of OT in human amnion, and Atosiban alone activates NF-κB and MAPKs thus upregulating downstream pro-labour genes...
February 10, 2017: Molecular Pharmacology
https://www.readbyqxmd.com/read/28170094/efficacy-of-freeze-dried-amnion-graft-following-hysteroscopic-adhesiolysis-of-severe-intrauterine-adhesions
#17
Lu Gan, Hua Duan, Fu-Qing Sun, Qian Xu, Yi-Qun Tang, Sha Wang
OBJECTIVE: To evaluate the efficacy of freeze-dried amnion graft for prevention of intrauterine adhesion (IUA) reformation after hysteroscopic adhesiolysis. METHODS: A prospective randomized controlled trial was conducted among 88 women with severe IUAs who underwent hysteroscopic adhesiolysis at Beijing Obstetrics and Gynecology Hospital between July 15, 2015, and July 1, 2016. All participants had a balloon inserted into the uterine cavity for 1 week. Sterilized freeze-dried amnion graft covered the balloon portion of the Foley catheter among patients allocated to the amnion group (n=44), whereas patients in the control group (n=44) did not receive the graft...
February 7, 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/28139754/intranasal-delivery-of-a-novel-amnion-cell-secretome-prevents-neuronal-damage-and-preserves-function-in-a-mouse-multiple-sclerosis-model
#18
Reas S Khan, Kimberly Dine, Bailey Bauman, Michael Lorentsen, Lisa Lin, Helayna Brown, Leah R Hanson, Aleta L Svitak, Howard Wessel, Larry Brown, Kenneth S Shindler
The ability of a novel intranasally delivered amnion cell derived biologic to suppress inflammation, prevent neuronal damage and preserve neurologic function in the experimental autoimmune encephalomyelitis animal model of multiple sclerosis was assessed. Currently, there are no existing optic nerve treatment methods for disease or trauma that result in permanent vision loss. Demyelinating optic nerve inflammation, termed optic neuritis, induces permanent visual dysfunction due to retinal ganglion cell damage in multiple sclerosis and experimental autoimmune encephalomyelitis...
January 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28117628/the-isolation-and-production-of-a-ready-to-use-product-the-amniotic-stem-cell-culture-in-accordance-with-gmp-regulations
#19
Diana Kitala, Marek Kawecki, Agnieszka Klama-Baryła, Wojciech Łabuś, Justyna Glik, Małgorzata Kraut, Marcelina Misiuga, Mariusz Nowak
According to the Committee for Advanced Therapies, amniotic stem cells were classified as an advanced therapy medicinal product. This work aims to standardize the isolation of amniotic stem cells and the selection of the optimal time of transplantation and cell application methods in burn patients according to the guidelines of the Good Manufacturing Practice. The placenta used in the study was sourced during a Cesarean section. The remnants of the amnion preparation were placed in a sterile container and transferred to a class B environment, where the primary cultures began...
January 24, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28112985/comparison-of-human-amniotic-chorionic-and-umbilical-cord-multipotent-mesenchymal-stem-cells-regarding-their-capacity-for-differentiation-toward-female-germ-cells
#20
Hamid Reza Asgari, Mohammad Akbari, Hossein Yazdekhasti, Zahra Rajabi, Shadan Navid, Fereshte Aliakbari, Niloufar Abbasi, Freidon Sargolzaei Aval, Alireza Shams, Mehdi Abbasi
Placenta harbors a plentiful source of various cells with stem cells or stem-like cell properties, which can be used in therapeutic procedures and research. Mesenchymal stem cells (MSCs) have attracted much attention due to their specific differentiation potential and tolerogenic properties. MSCs have been isolated from different parts of placenta; however, in this study, we isolated MSCs from amnion and chorion membrane, as well as umbilical cord (Wharton's jelly [WJ]) and compared their capacity regarding differentiation toward female germ cells under influence of 10 ng/mL BMP4...
February 2017: Cellular Reprogramming
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