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pertussis fermentation

Ali Nikdel, Richard D Braatz, Hector M Budman
Dynamic flux balance analysis (DFBA) has become an instrumental modeling tool for describing the dynamic behavior of bioprocesses. DFBA involves the maximization of a biologically meaningful objective subject to kinetic constraints on the rate of consumption/production of metabolites. In this paper, we propose a systematic data-based approach for finding both the biological objective function and a minimum set of active constraints necessary for matching the model predictions to the experimental data. The proposed algorithm accounts for the errors in the experiments and eliminates the need for ad hoc choices of objective function and constraints as done in previous studies...
May 2018: Bioprocess and Biosystems Engineering
I Kaji, Y Akiba, T Furuyama, D W Adelson, K Iwamoto, M Watanabe, A Kuwahara, J D Kaunitz
BACKGROUND: Short-chain fatty acids (SCFA) are microbial fermentation products absorbed by the colon. We recently reported that activation of the SCFA receptor termed free fatty acid receptor 3 (FFA3), expressed on cholinergic nerves, suppresses nicotinic acetylcholine receptor (nAChR)-mediated transepithelial anion secretion. This study aimed to clarify how activation of neurally expressed FFA3 affects colonic motor function. METHODS: FFA3-expressing myenteric neurons were identified by immunostaining; contractions of isolated circular muscle strips obtained from rat proximal colon were measured by isometric transducers...
January 2018: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
Vanessa Zavatti, Hector Budman, Raymond Legge, Melih Tamer
Fluorescence spectroscopy in combination with multivariate statistical methods was employed as a tool for monitoring the manufacturing process of pertactin (PRN), one of the virulence factors of Bordetella pertussis utilized in whopping cough vaccines. Fluorophores such as amino acids and co-enzymes were detected throughout the process. The fluorescence data collected at different stages of the fermentation and purification process were treated employing principal component analysis (PCA). Through PCA, it was feasible to identify sources of variability in PRN production...
June 2016: Bioprocess and Biosystems Engineering
Carolina Iraporda, Agustina Errea, David E Romanin, Delphine Cayet, Elba Pereyra, Omar Pignataro, Jean Claude Sirard, Graciela L Garrote, Analía G Abraham, Martín Rumbo
The use of short chain fatty acids to modulate gastrointestinal inflammatory conditions such as ulcerative colitis has produced encouraging results either in animal models or also in clinical trials. Identifying the key cellular and molecular targets of this activity will contribute to establish the appropriate combinations/targeting strategies to maximize the efficacy of anti-inflammatory interventions. In the present work, we evaluated in vitro the interaction of lactate, acetate, propionate and butyrate on cells relevant for innate immune response of the gastrointestinal tract...
October 2015: Immunobiology
Thakur Datta, Devender Sharma, Inderjeet Gandhoke, Shashi Khare, Arun Bhardwaj
Since introduction of the pertussis vaccine in 1940's the morbidity and mortality due to the infection has been markedly reduced all over the world. However the adverse effects of the inactivated whole cell pertussis vaccine like pain, swelling at the site of injection, fever, vomiting anorexia, persistent crying & drowsiness have been the cause of great concern, till date. Also the safety concerns over the use of thiomersal as an inactivating agent as well preservative have been raised in the recent past. Studies in many countries have been initiated to reduce or replace thiomersal & using other inactivating agents in the vaccines...
September 2011: Journal of Communicable Diseases
Hector Budman, Nilesh Patel, Melih Tamer, Walid Al-Gherwi
A mathematical model based on a dynamic metabolic flux balance (DMFB) is developed for a process of fed-batch fermentation of Bordetella pertussis. The model is based on the maximization of growth rate at each time interval subject to stoichiometric constraints. The model is calibrated and verified with experimental data obtained in two different bioreactor experimental systems. It was found that the model calibration was mostly sensitive to the consumption or production rates of tyrosine and, for high supplementation rates, to the consumption rate of glutamate...
March 2013: Biotechnology Progress
Yeon-Ju Kim, Myung Kyum Kim, Wan-Taek Im, Sathiyaraj Srinivasan, Deok-Chun Yang
A novel betaproteobacterium, designated strain Ch07(T), was isolated from granules from the wastewater-treatment bioreactor of an alcohol fermentation factory in South Korea. In order to determine its taxonomic position, the novel strain was characterized using a polyphasic approach. The new strain was Gram-negative, facultatively anaerobic, non-spore-forming, motile and short rod-shaped. 16S rRNA gene sequence analysis revealed that strain Ch07(T) belonged to the class Betaproteobacteria, being related to Pusillimonas noertemannii BN9(T) (gene sequence similarity 97...
June 2010: International Journal of Systematic and Evolutionary Microbiology
Elen A Perpetuo, Ivo Lebrun, Luis Juliano, Maria Aparecida Juliano, Maria Aparecida Sakauchi, Sally M A Prado
Proteases were identified and characterized from the culture supernatant of the C. diphtheriae and B. pertussis bacteria. The proteases were secreted in the media and detected at the end of the exponential growth phase. Activity was detected in some fluorescent substrates, based on selected protein sequences such as insuline beta-chain, bradykinin, and synaptobrevin. The proteases were purified by means of gel filtration chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the purified proteins indicated, for the main secreted proteins, an estimated molecular mass of 30 kDa in C...
2007: Preparative Biochemistry & Biotechnology
A P MacGowan
Moxifloxacin (Bay 12-8039) is a new 8 methoxy quinolone antibacterial. The MIC90 values are < or = 0.25 mg/l for Streptococcus pneumoniae (irrespective of penicillin susceptibility), Haemophilus influenzae (beta-lactamase positive or negative), Morexella catarrhalis, Bordetella pertussis, Legionella sp., Mycoplasma pneumoniae, Clamydia pneumoniae, Mycobacterium tuberculosis, methicillin-sensitive Staphylococcus aureus, beta-haemolytic streptococci (macrolide-sensitive or -resistant), Listeria sp., most Enterobacteriaceae, Salmonella sp...
February 1999: Expert Opinion on Investigational Drugs
J A Bogdan, J Nazario-Larrieu, J Sarwar, P Alexander, M S Blake
Pertussis toxin (Ptx) expression and secretion in Bordetella pertussis are regulated by a two-component signal transduction system encoded by the bvg regulatory locus. However, it is not known whether the metabolic pathways and growth state of the bacterium influence synthesis and secretion of Ptx and other virulence factors. We have observed a reduction in the concentration of Ptx per optical density unit midway in fermentation. Studies were conducted to identify possible factors causing this reduction and to develop culture conditions that optimize Ptx expression...
November 2001: Infection and Immunity
L V Miriasova, I A Basnak'ian, T V Kopaeva, I Iu Sarkisov
The processes of the cultivation of Bordetella pertussis, immobilized on polyurethane carrier in a fermenter, were carried out and studied. Acellular pertussis preparations were produced from the culture fluid obtained in the batch and multi-cycle cultivation processes with immobilized cells, as well as in the process with interrupted fermentation (for confirming the possibility of the preservation of cell viability). The content of protein and B. pertussis toxin in these preparations, as well as their leukocytes-stimulating and hemagglutinating activity, did not differ from similar characteristics of preparations obtained from culture fluid in homogeneous cultivation...
May 1999: Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii
J Germán Santiago, N Zamora, E de la Rosa, C Alba Carrión, P Padrón, M Hernández, M Betancourt, N Moretti
The production of Pertussis Vaccine was reevaluated at the Instituto Nacional de Higiene "Rafael Rangel" in order to optimise it in terms of vaccine yield, potency, specific toxicity and efficiency (cost per doses). Four different processes, using two culture media (Cohen-Wheeler and Fermentación Glutamato Prolina-1) and two types of bioreactors (25 L Fermentador Caracas and a 450 L industrial fermentor) were compared. Runs were started from freeze-dried strains (134 or 509) and continued until the obtention of the maximal yield...
1995: Acta Científica Venezolana
M Plaza Ramírez, J Ruiz Puente, M González Pacheco, L Recamier Palacios
No abstract text is available yet for this article.
November 1981: Salud Pública de México
O Galicia, H Villalva, A Pérez, S Pérez, A Medina
No abstract text is available yet for this article.
November 1981: Salud Pública de México
A Medina García, T A Martín Escobar, E T Reyes
No abstract text is available yet for this article.
November 1981: Salud Pública de México
L Recamier, A Medina, H Rodríguez
No abstract text is available yet for this article.
November 1981: Salud Pública de México
A Medina García, I Gómez Reyes
No abstract text is available yet for this article.
November 1981: Salud Pública de México
J Bellalou, E H Relyveld
Conditions for growing Bordetella pertussis bacteria in fermentors were studied for the purpose of producing highly protective vaccines. Bacteria with high protective potency were obtained in fermentors when the inoculum consisted of a dense suspension of young B. pertussis cells grown on Bordet-Gengou agar plates. When the time of cultivation in fermentors exceeded 30 h, bacteria harvested showed lower protective potency activity and lower levels of histamine sensitizing factor (HSF) and leukocytosis promoting factor (LPF), indicating that the optimal time for collection is at the end of the logarithmic growth phase...
July 1984: Annales de Microbiologie
D W Stainer, M J Scholte
No abstract text is available yet for this article.
October 1970: Journal of General Microbiology
A L Van Wezel
At a birth rate of about 150 million children per year, 300 to 400 million doses DTP-polio and 150 to 200 million doses of measles and BCG vaccines will be required annually for immunization of the world population against the target diseases as defined by the EPI of the World Health Organization. With the introduction of modern fermentation technologies production of these quantities is practically and economically feasible. The main problem is the administration of the vaccine to the target population. This may be achieved by application of a more condense and simplified vaccination schedule which has shown to be effective in clinical studies...
September 1985: Tropical and Geographical Medicine
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