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Hiie Nõlvak, Marika Truu, Kristjan Oopkaup, Kärt Kanger, Ivo Krustok, Emma Nehrenheim, Jaak Truu
Wastewater treatment systems receiving municipal wastewater are major dissemination nodes of antibiotic resistance genes (ARGs) between anthropogenic and natural environments. This study examined the fate of antibiotic resistome and class 1-3 integron-integrase genes in photobioreactors that were treating municipal wastewater diluted (70/30) with lake or tap water for the algal biomass production. A combined approach of metagenomic and quantitative (qPCR) analysis was undertaken. Municipal wastewater treatment in the photobioreactors led to reduced antibiotic resistome proportion, number of ARG subtypes, and abundances of individual ARGs in the bacterial community...
June 8, 2018: Water Research
Ronaldo Roberto Tait Caleffe, Stefany Rodrigues de Oliveira, Adriana Aparecida Sinópolis Gigliolli, Maria Claudia Colla Ruvolo-Takasusuki, Helio Conte
Larval therapy (LT) comprises the application of sterile Calliphoridae larvae for wound debridement, disinfection, and healing in humans and animals. Larval digestion plays a key role in LT, where the salivary glands and gut produce and secrete proteolytic and antimicrobial substances. The objective of this work was to bioprospect the salivary glands of Chrysomya megacephala (Fabricius, 1794) larvae, using ultrastructural, morphological, and histological observations, and the total protein electrophoretic profile...
June 8, 2018: Micron: the International Research and Review Journal for Microscopy
Tianyang Gao, Yingqiu Liang, Haiyu Tang, Li Quan
OBJECTIVE: This study was to determinate the expression of Tspan5 in tubal ectopic implantation sites and to explore the correlation of the expressive level of Tspan5 at maternal-fetal interface and the occurrence of tubal ectopic pregnancy. STUDY DESIGN: This is a retrospective study. Trophoblastic and endometrial tissues were collected from tubal ectopic pregnancy(Total of 40), and intrauterine pregnancy(Total of 41), who had voluntary abortion, non-pregnancy women(Total of 12), who recieved an diagnostic uterine curettage before IVF-ET for male infertility...
June 5, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Shan Liu, Zhaodi Zheng, Shuhua Ji, Tingting Liu, Yanhan Hou, Shasha Li, Guorong Li
Senescent cells display a senescence-associated secretory phenotype (SASP), which contributes to aging. Resveratrol, an activator of SIRT1, has anti-aging, anti-inflammatory, anti-oxidant, anti-free radical and other pharmacological effects. The genus of the annual fish Nothobranchius has become an emerging animal model for studying aging. However, the underlying mechanism for resveratrol to delay aging by SASP regulation has not been elucidated in vertebrates. In this study, the annual fish N. guentheri were fed with resveratrol for long-term treatment...
June 13, 2018: Fish & Shellfish Immunology
Diane R M Somlo, Nelson P Repenning, Abeel A Mangi
BACKGROUND: Contemporary reimbursement systems allow hospitals to maintain profitability by offering procedural services. However, increasing procedural volume can be met with structural inefficiencies that in turn cause delays, worse patient outcomes, and increased cost. METHODS: A multidisciplinary team assessed operations and outcomes at the Heart and Vascular Center at Yale New Haven Hospital, a tertiary academic medical center. Data were analyzed retrospectively to assess delays in transferring patients between care environments, patient outcomes, and cost...
June 13, 2018: Annals of Thoracic Surgery
Somayeh Mohammadi, Abdollah Salimi, Somayeh Hamde Qaddareh
We proposed an amplified FRET immunosensing for detection of CA15-3 tumor marker by highly biospecific interactions between CA 15-3 antigen and the corresponding antibody and aptamer. In this sandwich type immunoassay, CA15-3 antibody-functionalized carbon dots and AuNPs labeled PAMAM-Dendrimer/aptamer were used as donor/acceptor, respectively. When CA 15-3 Ag was added to homogenous immunoassay, the strong complex interaction between CA15-3 Ab-CA15-3 Ag- aptamer caused in more coming closer carbon dot and AuNPs and more decreasing fluorescence signal...
June 13, 2018: Analytical Biochemistry
Paul A Lapchak, Paul D Boitano, Geoffrey de Couto, Eduardo Marbán
Acute ischemic stroke is devastating to patients and their families because of few viable therapeutic options to promote recovery after reperfusion windows close. Recent breakthroughs in biotechnology have resulted in a reproducible patented process for the purification of extracellular vesicles (EVs) from human cardiosphere-derived cells (CDCs). Because CDC-EVs have many features potentially beneficial to treat acute ischemic stroke, CDC-EVs were evaluated in an established small-clot rabbit embolic stroke model, where clinically relevant end points were used to assess recovery in a more translational large animal model...
June 13, 2018: Experimental Neurology
Yolanda Wong Ying Yip, Tsz Chun Man, Chi Pui Pang, Mårten Erik Brelén
The aim of this study was to compare the quality of electroretinogram (ERG) recordings using a custom built active electrode with attached amplifier versus a standard (passive) ERG electrode. Scotopic and photopic ERG responses were recorded from five adult albino rabbits using a custom built active electrode on one eye and a passive electrode on the other. For the active electrode, the ERG-jet electrode (Universo S.A., La Chaux-De-Fonds, Switzerland) was used as the transducer with the cable cut short and soldered directly to the input of a customized amplifier...
June 13, 2018: Experimental Eye Research
Paulina Stolarek, Sylwia Różalska, Przemysław Bernat
Metarhizium robertsii, a butyltin-resistant filamentous fungus, can rapid and complete biodegradation of di- (DBT) and tributyltin (TBT) under conditions of intensive aeration and ascorbic acid supplementation. In this paper, lipidomic investigations were performed to find the membrane adaptations necessary for effective butyltins degradation. HPLC-MS/MS analysis showed that the phospholipid profile was greatly modified during M. robertsii batch cultivation (pO2  ≥ 20%), contributing to increased membrane fluidity and facilitated mass transfer, which could enhance butyltins biodegradation...
June 13, 2018: Biochimica et Biophysica Acta
G N E Maino, C Valles, A Santos, A Pascual, C Esquinas, J Nart
OBJECTIVES: The aims of this randomized clinical trial were to investigate the influence of the suturing technique and the thickness of the pre- and postoperative palatal and flap mucosa on the early healing of the palate after harvesting a connective tissue graft. Furthermore, patient pain perception was evaluated. MATERIAL AND METHODS: A subepithelial connective tissue graft was obtained from the palate with a single horizontal incision technique. Patients were randomly assigned to continuous interlocking suture group or criss-cross suture group...
June 16, 2018: Journal of Clinical Periodontology
Paul Ken Leong Chin, Philip George Drennan, Sharon Jane Gardiner, Mei Zhang, Simon Charles Dalton, Stephen T Chambers, Evan James Begg
BACKGROUND: Flucloxacillin dosing may be guided by measurement of its total plasma concentrations. Flucloxacillin is highly protein bound with fraction unbound in plasma (fu ) of around 0.04 in healthy individuals. The utility of measuring unbound flucloxacillin concentrations for patients outside the intensive care unit (ICU) is not established. AIMS: To compare flucloxacillin fu in non-ICU hospitalised patients against healthy volunteers. To examine the performance of a published model for predicting unbound concentrations, using total flucloxacillin and plasma albumin concentrations...
June 16, 2018: British Journal of Clinical Pharmacology
M A Riedijk, D M J Milstein
OBJECTIVE: Procedural sedation with propofol is widely used in the pediatric population. A well-known side effect of propofol is a decrease of peripheral vascular resistance resulting in hypotension, but little is known about the effects on the microcirculation in humans. We aimed to evaluate the effects of propofol on the sublingual microcirculatory perfusion by continuous video imaging in pediatric patients undergoing procedural sedation. METHODS: Patients admitted to the Pediatric Intensive Care Unit for procedural sedation were recruited...
June 16, 2018: Microcirculation: the Official Journal of the Microcirculatory Society, Inc
Bernhard Liedl, Klaus Goeschen, Suzette E Sutherland, Jean-Paul Roovers, Alexander Yassouridis
OBJECTIVE: The objective of this interventional study was to examine extent and intensity of the coexistence of overactive bladder (OAB) symptoms in women with pelvic organ prolapse (POP) and to evaluate the likelihood of OAB symptom improvement after surgical POP reconstruction in a period of two years. PATIENTS AND METHODS: The effectiveness of the transvaginal, single-incision Elevate@ technique for anatomical cure of anterior/apical and posterior/apical vaginal prolapse has been previously reported following a prospective, multicenter study...
June 16, 2018: BJU International
Tantry Maulina, Hedy Diana, Arief Cahyanto, Amaliya
BACKGROUND: Curcumin is a turmeric contained active ingredient that has been proven to be effective in treating pain and inflammation due to its analgesic as well as anti-inflammation potential. Odontectomy, on the other hand, has been well known for its post-procedure acute inflammation pain. OBJECTIVE: The aim of the current study was to evaluate the efficacy of curcuminoid in treating acute inflammation post-operative pain in the post-surgical removal of impacted third molars patients...
June 16, 2018: Journal of Oral Rehabilitation
May Y Choi, Cheryl Barnabe, Claire E Barber, Vivian Bykerk, Janet E Pope, Glen S Hazlewood
OBJECTIVE: Randomized controlled trials (RCTs) exist along a spectrum, from explanatory, designed to evaluate interventions under ideal conditions, to pragmatic, designed to reflect usual care. This study assessed the pragmaticism of RCTs of advanced therapeutics in rheumatoid arthritis (RA). METHODS: A systematic review was conducted to identify RA RCTs comparing biologic or targeted synthetic therapy in combination with methotrexate, to placebo or any other DMARD(s)...
June 15, 2018: Arthritis Care & Research
Riccardo Flamini, Marta Menicatti, Mirko De Rosso, Massimo Gardiman, Christine Mayr, Pallecchi Marco, Giovanna Danza, Gianluca Bartolucci
Monoterpene-glycosides are important aroma precursors which, undergo hydrolysis, confer intense floral notes to the wines. Therefore, the knowledge of the nature of the sugar residues and the structure of these molecules is of great interest. In present study, liquid chromatography (LC) separation coupled with different mass spectrometry (MS) experiments for the characterization of these compounds were explored. The LC parameters were tuned to optimize the resolution between the analytes present in grape sample extracts...
June 15, 2018: Journal of Mass Spectrometry: JMS
Peter Bigler, Julien Furrer
Long-Range Heteronuclear Single Quantum Correlation (LR-HSQC) experiments may be applied as an alternative to Heteronuclear Multiple-Bond correlation (HMBC) experiments for detecting long-range correlations, but has never enjoyed popularity for that purpose. To the best of our knowledge, the exact reasons have not yet been fully established. For both experiments it is widely accepted that the evolution of proton-proton homonuclear couplings JHH' during the polarization transfer delays Δ leads to significant losses, and that the intensity of the observable coherence is zero when JHH' matches the condition Δ = 0...
June 15, 2018: Magnetic Resonance in Chemistry: MRC
Hiroyuki Ohbe, Taisuke Jo, Hiroki Matsui, Kiyohide Fushimi, Hideo Yasunaga
INTRODUCTION: In contrast to information on the effects of organophosphate, pesticide, or environmental exposures, data on cholinergic crisis caused by pharmaceutical cholinesterase inhibitors are sparse. The present study aimed to describe the characteristics, demographics, and mortality of patients with cholinergic crisis caused by pharmaceutical cholinesterase inhibitors using a nationwide inpatient database in Japan. METHODS: We identified patients diagnosed with cholinergic crisis as a result of taking cholinesterase inhibitor medications in the Japanese Diagnosis Procedure Combination inpatient database from July 2010 to March 2016...
June 15, 2018: Journal of Medical Toxicology: Official Journal of the American College of Medical Toxicology
Yoav Bichovsky, Leonid Koyfman, Michael Friger, Boris Kirshtein, Abraham Borer, Gilbert Sebbag, Dmitry Frank, Amit Frenkel, Jochanan G Peiser, Moti Klein, Evgeni Brotfain
PURPOSE: Peritonitis is a major complication of bariatric surgery due to direct damage to the natural barriers to infection. Most such secondary peritoneal infections are caused by Gram-negative microorganisms; however, under certain conditions, Candida species can infect the peritoneal cavity following bariatric surgery. MATERIALS AND METHODS: We retrospectively analyzed the clinical and microbiological data of morbidly obese patients who suffered infectious complications following laparoscopic sleeve gastrectomy (LSG) at the Soroka Medical Center between January 2010 and June 2015...
June 15, 2018: Obesity Surgery
M Weigeldt, M Paul, S Schulz-Drost, M D Schmittner
The management of anesthesia plays a central role in the treatment of thoracic trauma, both in the initial phase when safeguarding the difficult airway and in the intensive care unit. A rapid transfer to a trauma center should be considered in order to recognize and treat organ dysfunction in time. Development of atelectasis, pneumonia and acute lung failure are common pulmonary complications. Non-invasive ventilation combined with physiotherapy and respiratory training can help to minimize these pulmonary complications...
June 15, 2018: Der Unfallchirurg
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