Zhengyu Jiang, Feijing Wu, Pasquale Laise, Tanaka Takayuki, Fu Na, Woosook Kim, Hiroki Kobayashi, Wenju Chang, Ryota Takahashi, Giovanni Valenti, Masaki Sunagawa, Ruth A White, Marina Macchini, Bernhard W Renz, Moritz Middelhoff, Yoku Hayakawa, Zinaida A Dubeykovskaya, Xiangtian Tan, Timothy H Chu, Karan Nagar, Yagnesh Tailor, Bryana R Belin, Akanksha Anand, Samuel Asfaha, Michael O Finlayson, Alina C Iuga, Andrea Califano, Timothy C Wang
While adult pancreatic stem cells are thought not to exist, it is now appreciated that the acinar compartment harbors progenitors, including tissue-repairing facultative progenitors (FPs). Here, we study a pancreatic acinar population marked by trefoil factor 2 (Tff2) expression. Long-term lineage tracing and single-cell RNA sequencing (scRNA-seq) analysis of Tff2-DTR-CreERT2 -targeted cells defines a transit-amplifying progenitor (TAP) population that contributes to normal homeostasis. Following acute and chronic injury, Tff2+ cells, distinct from FPs, undergo depopulation but are eventually replenished...
August 3, 2023: Cell Stem Cell