Natthaporn Sueangoen, Harald Grove, Nisa Chuangchot, Jaturawitt Prasopsiri, Thanyada Rungrotmongkol, Kamonpan Sanachai, Nitchakan Darai, Suyanee Thongchot, Prapat Suriyaphol, Doonyapat Sa-Nguanraksa, Peti Thuwajit, Pa-Thai Yenchitsomanus, Chanitra Thuwajit
Breast cancer stands as a formidable global health challenge for women. While neoantigens exhibit efficacy in activating T cells specific to cancer and instigating anti-tumor immune responses, the accuracy of neoantigen prediction remains suboptimal. In this study, we identified neoantigens from the patient-derived breast cancer cells, PC-B-142CA and PC-B-148CA cells, utilizing whole-genome and RNA sequencing. The pVAC-Seq pipeline was employed, with minor modification incorporating criteria (1) binding affinity of mutant (MT) peptide with HLA (IC50 MT) ≤ 500 nm in 3 of 5 algorithms and (2) IC50 wild type (WT)/MT > 1...
February 13, 2024: Cancer Immunology, Immunotherapy: CII