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https://www.readbyqxmd.com/read/28231164/essential-oil-characterization-of-thymus-vulgaris-from-various-geographical-locations
#1
Prabodh Satyal, Brittney L Murray, Robert L McFeeters, William N Setzer
Thyme (Thymus vulgaris L.) is a commonly used flavoring agent and medicinal herb. Several chemotypes of thyme, based on essential oil compositions, have been established, including (1) linalool; (2) borneol; (3) geraniol; (4) sabinene hydrate; (5) thymol; (6) carvacrol, as well as a number of multiple-component chemotypes. In this work, two different T. vulgaris essential oils were obtained from France and two were obtained from Serbia. The chemical compositions were determined using gas chromatography-mass spectrometry...
October 27, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28223069/evaluation-of-antibacterial-potential-and-toxicity-of-plant-volatile-compounds-using-new-broth-microdilution-volatilization-method-and-modified-mtt-assay
#2
Marketa Houdkova, Johana Rondevaldova, Ivo Doskocil, Ladislav Kokoska
With aim to develop effective proof-of-concept approach which can be used in a development of new preparations for the inhalation therapy, we designed a new screening method for simple and rapid simultaneous determination of antibacterial potential of plant volatiles in the liquid and the vapour phase at different concentrations. In addition, EVA (ethylene vinyl acetate) capmat™ as vapour barrier cover was used as reliable modification of thiazolyl blue tetrazolium bromide (MTT) assay for cytotoxicity testing of volatiles on microtiter plates...
February 18, 2017: Fitoterapia
https://www.readbyqxmd.com/read/28221940/development-of-multifunctional-active-film-and-its-application-in-modified-atmosphere-packaging-of-shiitake-mushrooms
#3
Hong Jiang Wang, Duck Soon An, Dong Sun Lee
Agar-based films with multiple functions (CO2 absorption, water vapor absorption, and antimicrobial activity) were developed, tested for their properties, and then applied to the packaging of fresh shiitake mushrooms as an insert label. The films were cast from an agar-based aqueous solution containing a dissolving plasticizer (glycerol), a CO2 absorbent (sodium carbonate [SC] alone or a combination of SC and sodium glycinate [SC-SG]), and a volatile antimicrobial agent (carvacrol [CRV]). The agar of the film matrix is designed to serve as a water vapor absorbent...
September 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221905/efficacy-of-plant-derived-antimicrobials-in-controlling-enterohemorrhagic-escherichia-coli-virulence-in-vitro
#4
Sangeetha Ananda Baskaran, Anup Kollanoor-Johny, Meera Surendran Nair, Kumar Venkitanarayanan
Escherichia coli O157:H7 is a major foodborne pathogen that can cause serious human illness characterized by hemorrhagic diarrhea and kidney failure. The pathology of enterohemorrhagic E. coli O157:H7 (EHEC) infection is primarily mediated by verotoxins, which bind to the globotriaosylceramide receptor on host cells. Antibiotics are contraindicated for treating EHEC infection because they lead to increased verotoxin release, thereby increasing the risk of renal failure and death in patients. Thus, alternative strategies are needed for controlling EHEC infections in humans...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28216901/technological-evaluation-of-emulsions-containing-the-volatile-oil-from-leaves-of-plectranthus-amboinicus-lour
#5
Pablo Queiroz Lopes, Fabíola Bernardo Carneiro, Ana Letícia Braz de Sousa, Sócrates Golziodo Santos, Elquio Eleamen Oliveira, Luiz Alberto Lira Soares
BACKGROUND: Plectranthus amboinicus Lour is a species which is widespread throughout tropical countries where it is widely used against respiratory tract disorders such as bronchodilator, antitussive, and expectorant conditions. OBJECTIVE: This study aims to characterize the essential oil of P. amboinicus (PaEO) and produce and evaluate emulsions containing PaEO. MATERIALS AND METHODS: The essential oil was characterized by physical-chemical analyses for density, refractive index, 90% ethanol solubility, color, appearance, and identification by gas chromatography coupled to mass spectrometry detection...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28215890/effect-of-oral-calcium-administration-on-the-cure-and-reproductive-performance-of-holstein-cows-diagnosed-with-puerperal-metritis
#6
Pablo Pinedo, Juan Velez, Gilberto Solano, Nelson Rodriguez, Julianne Naves, Gustavo M Schuenemann, Carlos Risco
Our objective was to evaluate the effect of oral calcium administration on clinical cure, survival, subsequent presentation of peripartal health disorders, and reproductive performance of Holstein cows diagnosed with puerperal metritis (PM) under certified organic management. A second objective was to evaluate the metabolic status at calving and at the time of PM diagnosis (d 0) in affected and matched healthy cows. Cows diagnosed with PM (n = 200) were assigned randomly to receive 1 of 2 treatments: (1) control received 3...
February 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28191274/the-neuroprotective-effects-of-carvacrol-on-ethanol-induced-hippocampal-neurons-impairment-via-the-antioxidative-and-antiapoptotic-pathways
#7
Peng Wang, Qian Luo, Hui Qiao, Hui Ding, Yonggang Cao, Juan Yu, Ruxia Liu, Qianlong Zhang, Hui Zhu, Lihui Qu
Chronic alcohol consumption causes hippocampal neuronal impairment, which is associated with oxidative stress and apoptosis. Carvacrol is a major monoterpenic phenol found in essential oils from the family Labiatae and has antioxidative stress and antiapoptosis actions. However, the protective effects of carvacrol in ethanol-induced hippocampal neuronal impairment have not been fully understood. We explored the neuroprotective effects of carvacrol in vivo and in vitro. Male C57BL/6 mice were exposed to 35% ethanol for 4 weeks to establish ethanol model in vivo, and hippocampal neuron injury was simulated by 200 mM ethanol in vitro...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28178379/antigenotoxic-effect-against-ultraviolet-radiation-induced-dna-damage-of-the-essential-oils-from-lippia-species
#8
Nathalia Quintero Ruiz, Yuri Cordoba Campo, Elena E Stashenko, Jorge Luis Fuentes
The antigenotoxicity against ultraviolet radiation (UV) -induced DNA damage of essential oils (EO) from Lippia species was studied using SOS Chromotest. Based on the minimum concentration that significantly inhibits genotoxicity, the genoprotective potential of EO from highest to lowest was L. alba, citral -rich chemotype (RC) > L. graveolens, thymol-RC ≈ L. origanoides, thymol-RC ≈ L. origanoides, carvacrol-RC > L. micromera, thymol-RC ≈ L. citriodora, citral-RC > L. alba, myrcenone-RC > L...
February 8, 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/28177660/carvacrol-attenuates-cyclophosphamide-induced-oxidative-stress-in-rat-kidney
#9
Sibel Gunes, Adnan Ayhanci, Varol Sahinturk, Diler Us Altay, Ruhi Uyar
Cyclophosphamide (CP) is an antineoplastic drug inducing kidney damage via producing oxidative stress. Carvacrol (CAR) has antioxidative effect and we postulated that it can be protective against CP-induced nephrotoxicity. Six groups (n=7) of rats (control, 100 mg/kg CP, CP+5 and CP+10 mg/kg CAR and 5 and 10 mg/kg CAR) were injected intraperitoneally. Serum malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), creatinine (CRE), total antioxidant capacity (TAC) and total oxidant state (TOS) were measured, and oxidative stress indexes (OSI) were calculated...
February 3, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28129937/determination-of-cinnamaldehyde-carvacrol-and-thymol-in-feedstuff-additives-by-pressurized-liquid-extraction-followed-by-gas-chromatography-mass-spectrometry
#10
Marta Jiménez-Salcedo, María Teresa Tena
Specific blends of essential oils (BEOs) are promising substitutes for antibiotics to promote livestock performance and to reduce the incidence of intestinal disorders. Microencapsulation of BEOs has shown to improve their stability, bioavailability and to control their release rate once they are added to the feedstuff. The development and validation of a method for determining essential oil components such as carvacrol, thymol and cinnamaldehyde in a microencapsulated material used as feed additive is presented...
March 3, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28106093/insecticidal-toxicities-of-carvacrol-and-thymol-derived-from-thymus-vulgaris-lin-against-pochazia-shantungensis-chou-lu-newly-recorded-pest
#11
Jun-Hwan Park, Ye-Jin Jeon, Chi-Hoon Lee, Namhyun Chung, Hoi-Seon Lee
The insecticidal toxicities of five essential oils against Pochazia shantungensis adults and nymphs, newly recorded pests, were evaluated. The LC50 values of Thymus vulgaris, Ruta graveolens, Citrus aurantium, Leptospermum petersonii and Achillea millefolium oils were recorded as 57.48, 84.44, 92.58, 113.26 and 125.78 mg/L, respectively, against P. shantungensis nymphs using the leaf dipping bioassay, and 75.80, 109.86, 113.26, 145.06 and 153.74 mg/L, respectively, against P. shantungensis adults using the spray bioassay method...
January 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28103733/essential-oil-composition-and-antinociceptive-activity-of-thymus-capitatus
#12
Juan Carlos Ramos Gonçalves, Danilo Andrade de Meneses, Aliny Pereira de Vasconcelos, Celyane Alves Piauilino, Fernanda Regina de Castro Almeida, Edoardo Marco Napoli, Giuseppe Ruberto, Demetrius Antônio Machado de Araújo
CONTEXT: The essential oil (EO) from Thymus capitatus Hoff. et Link. (Lamiaceae) has been traditionally used for its medicinal properties, such as anti-inflammatory, analgesic, antioxidant and antimicrobial properties. OBJECTIVE: Characterize the constituents from T. capitatus EO and further evaluate the antinociceptive activity by in vivo and in vitro procedures. MATERIALS AND METHODS: Gas chromatography-mass spectrometry was used to identify and quantify the constituents of the T...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28089589/synthesis-anticholinesterase-activity-and-molecular-modeling-study-of-novel-carbamate-substituted-thymol-carvacrol-derivatives
#13
Belma Zengin Kurt, Isil Gazioglu, Aydan Dag, Ramin Ekhteiari Salmas, Gülru Kayık, Serdar Durdagi, Fatih Sonmez
New thymol and carvacrol derivatives with the carbamate moiety were synthesized and their inhibitory effects on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) were evaluated. 5-isopropyl-2-methylphenyl(3-fluorophenyl)carbamate (29) was found to be the most potent AChE inhibitor with IC50 values of 2.22μM, and 5-isopropyl-2-methylphenyl (4-fluorophenyl)carbamate (30) exhibited the strongest inhibition against BuChE with IC50 value of 0.02μM. Additionally, the result of H4IIE hepatoma cell toxicity assay for compounds 18, 20, 29, 30 and 35 showed negligible cell death at 0...
December 26, 2016: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28078244/carvacrol-attenuates-serum-levels-of-total-protein-phospholipase-a2-and-histamine-in-asthmatic-guinea-pig
#14
Mohammad Hossein Boskabady, Sedigheh Jalali, Negin Yahyazadeh, Mostafa Boskabady
OBJECTIVE: Pharmacological effects of carvacrol such as its anti-inflammatory activities have been shows. In this study the effects of carvacrol on serum levels of total protein (TP), phospholipase A2 (PLA2) and histamine in sensitized guinea pigs was evaluated. MATERIALS AND METHODS: Sensitized guinea pigs were given drinking water alone (group S), drinking water containing three concentrations of carvacrol (40, 80 and 160 µg/ml) or dexamethasone. Serum levels of TP, PLA2 and histamine were examined I all sensitized groups as well as a non-sensitized control group (n=6 for each group)...
November 2016: Avicenna Journal of Phytomedicine
https://www.readbyqxmd.com/read/28063330/endolysin-lyssa97-is-synergistic-with-carvacrol-in-controlling-staphylococcus-aureus-in-foods
#15
Yoonjee Chang, Hyunjin Yoon, Dong-Hyun Kang, Pahn-Shick Chang, Sangryeol Ryu
LysSA97 is an endolysin encoded by the bacteriophage SA97, the genome sequence of which has been recently revealed. LysSA97 has lytic activity against a variety of Staphylococcus strains that cause foodborne illness. In order to improve its potential as a biocontrol agent against Staphylococcus, various types of essential oil-derived active compounds were tested in combination with LysSA97; carvacrol exhibited significant synergistic effects when combined with LysSA97. The synergistic antimicrobial activity between endolysin and carvacrol in food products, including milk and beef, were investigated...
March 6, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28062311/trpa1-and-trpv1-antagonists-do-not-inhibit-human-acidosis-induced-pain
#16
Matthias G Schwarz, Barbara Namer, Peter W Reeh, Michael J M Fischer
: Acidosis occurs in a variety of pathophysiological and painful conditions where it is thought to excite or contribute to excitation of nociceptive neurons. Despite potential clinical relevance the principal receptor for sensing acidosis is unclear, but several receptors have been proposed. We investigated the contribution of the acid-sensing ion channels, transient receptor potential vanilloid type 1 (TRPV1) and transient receptor potential ankyrin type 1 (TRPA1) to peripheral pain signaling...
January 3, 2017: Journal of Pain: Official Journal of the American Pain Society
https://www.readbyqxmd.com/read/28062292/use-of-carvacrol-thymol-and-eugenol-for-biofilm-eradication-and-resistance-modifying-susceptibility-of-salmonella-enterica-serovar-typhimurium-strains-to-nalidixic-acid
#17
Hanene Miladi, Tarek Zmantar, Bochra Kouidhi, Yassine Chaabouni, Kacem Mahdouani, Amina Bakhrouf, Kamel Chaieb
The Aims of the study was to evaluate the antibacterial susceptibility and the biofilm eradication of three natural compounds carvacrol (CAR), thymol (TH) and eugenol (EUG), alone or in combination with nalidixic acid (NA) against twelve Salmonella Typhimurium strains. The minimum inhibitory concentration (MIC) and the minimum biofilm eradication concentration (BEC50) of the tested compounds (CAR, TH and EUG) and their combinations with NA were evaluated. In order to assess whether these bacteria had active efflux pumps, ethidium bromide (EtBr) accumulation assays was achieved using spectrophotometric accumulation assays...
March 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28055127/lippia-origanoides-essential-oil-an-efficient-and-safe-alternative-to-preserve-food-cosmetic-and-pharmaceutical-products
#18
Camila Hernandes, Edieidia Souza Pina, Silvia H Taleb-Contini, Bianca Waléria Bertoni, Ivanice Maria Cestari, Lívia Greghi Espanha, Eliana A Varanda, Katyana França Bonini Camilo, Edson Zangiacome Martinez, Suzelei de Castro França, Ana Maria Soares Pereira
AIM: The aim of this work was to evaluated the efficacy and safety of Lippia origanoides essential oil as a preservative in industrial products. METHODS AND RESULTS: The composition, antimicrobial activity, mutagenic and toxic potential of L. origanoides was determined. Then the effect of essential oil as preservative in food, cosmetics and pharmaceutical products was evaluated. The essential oil of L. origanoides consisted mainly of oxygenated monoterpenes (38...
January 5, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28024521/a-novel-interpretation-of-the-fractional-inhibitory-concentration-index-the-case-origanum-vulgare-l-and-leptospermum-scoparium-j-r-et-g-forst-essential-oils-against-staphylococcus-aureus-strains
#19
Filippo Fratini, Simone Mancini, Barbara Turchi, Elisabetta Friscia, Luisa Pistelli, Giulia Giusti, Domenico Cerri
Origanum vulgare (oregano) and Leptospermum scoparium (manuka) were traditionally employed as natural remedies for infected wounds and skin injuries where Staphylococcus aureus is mainly involved. The first aim of this study was to investigate oregano and manuka essential oils (EOs) chemical compositions and evaluate their antibacterial activity (MIC, Minimum Inhibitory Concentration) against fourteen S. aureus wild strains. The second aim was to evaluate the antibacterial activities of oregano and manuka EOs mixed in different combination (FIC, Fractional Inhibitory Concentration) with an improved chequerboard technique...
January 2017: Microbiological Research
https://www.readbyqxmd.com/read/28012625/short-communication-interaction-of-the-isomers-carvacrol-and-thymol-with-the-antibiotics-doxycycline-and-tilmicosin-in-vitro-effects-against-pathogenic-bacteria-commonly-found-in-the-respiratory-tract-of-calves
#20
W Kissels, X Wu, R R Santos
Bovine respiratory disease is the major problem faced by cattle, specially calves, leading to reduced animal performance and increased mortality, consequently causing important economic losses. Hence, calves must be submitted to antibiotic therapy to counteract this infection usually initiated by the combination of environmental stress factors and viral infection, altering the animal's defense mechanism, and thus allowing lung colonization by the opportunistic bacteria Mannheimia haemolytica and Pasteurella multocida...
February 2017: Journal of Dairy Science
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