keyword
MENU ▼
Read by QxMD icon Read
search

autophagy and heat shock protein

keyword
https://www.readbyqxmd.com/read/27928720/cardioprotection-of-exercise-preconditioning-involving-heat-shock-protein-70-and-concurrent-autophagy-a-potential-chaperone-assisted-selective-macroautophagy-effect
#1
Yang Yuan, Shan-Shan Pan, Yu-Jun Shen
It has been confirmed that exercise preconditioning (EP) has a protective effect on acute cardiovascular stress. However, how Hsp70 participates in EP-induced cardioprotection is unknown. EP may involve Hsp70 to repair unfolded proteins or may also stabilize the function of the endoplasmic reticulum via Hsp70-related autophagy to work on a protective formation. Our EP protocol involves four periods of 10 min running with 10 min recovery intervals. We added a period of exhaustive running to test this protective effect, using histology and molecular biotechnology methods to detect related markers...
December 7, 2016: Journal of Physiological Sciences: JPS
https://www.readbyqxmd.com/read/27896217/adapt-recycle-and-move-on-proteostasis-and-trafficking-mechanisms-in-melanoma
#2
REVIEW
Seyma Demirsoy, Shaun Martin, Hannelore Maes, Patrizia Agostinis
Melanoma has emerged as a paradigm of a highly aggressive and plastic cancer, capable to co-opt the tumor stroma in order to adapt to the hostile microenvironment, suppress immunosurveillance mechanisms, and disseminate. In particular, oncogene- and aneuploidy-driven dysregulations of proteostasis in melanoma cells impose a rewiring of central proteostatic processes, such as the heat shock and unfolded protein responses, autophagy, and the endo-lysosomal system, to avoid proteotoxicity. Research over the past decade has indicated that alterations in key nodes of these proteostasis pathways act in conjunction with crucial oncogenic drivers to increase intrinsic adaptations of melanoma cells against proteotoxic stress, modulate the high metabolic demand of these cancer cells and the interface with other stromal cells, through the heightened release of soluble factors or exosomes...
2016: Frontiers in Oncology
https://www.readbyqxmd.com/read/27874054/combinational-immunotherapy-with-allo-dribble-vaccines-and-anti-ox40-co-stimulation-leads-to-generation-of-cross-reactive-effector-t-cells-and-tumor-regression
#3
Guangjie Yu, Yuhuan Li, Zhihua Cui, Nicholas P Morris, Andrew D Weinberg, Bernard A Fox, Walter J Urba, Lixin Wang, Hong-Ming Hu
It is well-known that vaccines comprising of irradiated whole tumor cells or tumor-derived heat shock proteins can generate tumor-specific immune responses. In contrast, we showed recently that vaccines composed of autophagosomes (DRibbles) derived from syngeneic sarcomas could induce cross-reactive T-cell responses and cross-protection against the tumor. This unusual property of DRibbles was related to the selective recruitment of defective ribosomal products (DRiPs) and other short-lived proteins (SLiPs) into autophagosomes via sequestosome (SQSTM1, p62) mediated association of ubiquitinated SLiPs to the autophagy gene product LC3...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27870869/glucose-starvation-alters-heat-shock-response-leading-to-death-of-wild-type-cells-and-survival-of-map-kinase-signaling-mutant
#4
Nora Plesofsky, LeeAnn Higgins, Todd Markowski, Robert Brambl
A moderate heat shock induces Neurospora crassa to synthesize large quantities of heat shock proteins that are protective against higher, otherwise lethal temperatures. However, wild type cells do not survive when carbohydrate deprivation is added to heat shock. In contrast, a mutant strain defective in a stress-activated protein kinase does survive the combined stresses. In order to understand the basis for this difference in survival, we have determined the relative levels of detected proteins in the mutant and wild type strain during dual stress, and we have identified gene transcripts in both strains whose quantities change in response to heat shock or dual stress...
2016: PloS One
https://www.readbyqxmd.com/read/27846364/hsf1-stress-response-pathway-regulates-autophagy-receptor-sqstm1-p62-associated-proteostasis
#5
Yoshihisa Watanabe, Atsushi Tsujimura, Katsutoshi Taguchi, Masaki Tanaka
Proteostasis is important for protecting cells from harmful proteins and is mainly controlled by the HSF1 (heat shock transcription factor 1) stress response pathway. This pathway facilitates protein refolding by molecular chaperones; however, it is unclear whether it functions in autophagy or inclusion formation. The autophagy receptor SQSTM1/p62 is involved in selective autophagic clearance and inclusion formation by harmful proteins, and its phosphorylation at S349, S403, and S407 is required for binding to substrates...
November 15, 2016: Autophagy
https://www.readbyqxmd.com/read/27805285/survivin-inhibitor-ym155-induces-mitochondrial-dysfunction-autophagy-dna-damage-and-apoptosis-in-bcl-xl-silenced-glioma-cell-lines
#6
Esther P Jane, Daniel R Premkumar, Philip A Sutera, Jonathon M Cavaleri, Ian F Pollack
Because the anti-apoptotic protein Bcl-xL is overexpressed in glioma, one might expect that inhibiting or silencing this gene would promote tumor cell killing. However, our studies have shown that this approach has limited independent activity, but may tip the balance in favor of apoptosis induction in response to other therapeutic interventions. To address this issue, we performed a pharmacological screen using a panel of signaling inhibitors and chemotherapeutic agents in Bcl-xL silenced cells. Although limited apoptosis induction was observed with a series of inhibitors for receptor tyrosine kinases, PKC inhibitors, Src family members, JAK/STAT, histone deacetylase, the PI3K/Akt/mTOR pathway, MAP kinase, CDK, heat shock proteins, proteasomal processing, and various conventional chemotherapeutic agents, we observed a dramatic potentiation of apoptosis in Bcl-xL silenced cells with the survivin inhibitor, YM155...
November 2, 2016: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/27744332/the-function-of-small-heat-shock-proteins-and-their-implication-in-proteostasis
#7
Annika Strauch, Martin Haslbeck
All organisms rely on a conserved cellular machinery supporting and controlling the life cycle of proteins: the proteostasis network. Within this network, the main players that determine the fate of proteins are molecular chaperones, the ubiquitin-proteasome and the lysosome-autophagy systems. sHsps (small heat-shock proteins) represent one family of molecular chaperones found in all domains of life. They prevent irreversible aggregation of unfolded proteins and maintain proteostasis by stabilizing promiscuously a variety of non-native proteins in an ATP-independent manner...
October 15, 2016: Essays in Biochemistry
https://www.readbyqxmd.com/read/27726101/lapatinib-resistance-in-her2-cancers-latest-findings-and-new-concepts-on-molecular-mechanisms
#8
Huiping Shi, Weili Zhang, Qiaoming Zhi, Min Jiang
In the era of new and mostly effective molecular targeted therapies, human epidermal growth factor receptor 2 positive (HER2+) cancers are still intractable diseases. Lapatinib, a dual epidermal growth factor receptor (EGFR) and HER2 tyrosine kinase inhibitor, has greatly improved breast cancer prognosis in recent years after the initial introduction of trastuzumab (Herceptin). However, clinical evidence indicates the existence of both primary unresponsiveness and secondary lapatinib resistance, which leads to the failure of this agent in HER2+ cancer patients...
October 10, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27702699/targeting-chaperones-heat-shock-factor-1-and-unfolded-protein-response-promising-therapeutic-approaches-for-neurodegenerative-disorders
#9
Shambhunath Bose, Jungsook Cho
Protein misfolding, which is known to cause several serious diseases, is an emerging field that addresses multiple therapeutic areas. Misfolding of a disease-specific protein in the central nervous system ultimately results in the formation of toxic aggregates that may accumulate in the brain, leading to neuronal cell death and dysfunction, and associated clinical manifestations. A large number of neurodegenerative diseases in humans, including Alzheimer's, Parkinson's, Huntington's, and prion diseases, are primarily caused by protein misfolding and aggregation...
October 1, 2016: Ageing Research Reviews
https://www.readbyqxmd.com/read/27666506/inhibition-of-autophagy-sensitises-cells-to-hydrogen-peroxide-induced-apoptosis-protective-effect-of-mild-thermotolerance-acquired-at-40%C3%A2-c
#10
Maureen Redza-Dutordoir, Sarah Kassis, Hou Ve, Mélanie Grondin, Diana A Averill-Bates
Various toxic compounds produce reactive oxygen species, resulting in oxidative stress that threatens cellular homeostasis. Yet, lower doses of stress can stimulate defence systems allowing cell survival, whereas intense stress activates cell death pathways such as apoptosis. Mild thermal stress (40°C, 3h) induces thermotolerance, an adaptive survival response that renders cells less sensitive to subsequent toxic stress, by activating defence systems like heat shock proteins, antioxidants, anti-apoptotic and ER-stress factors...
December 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27560450/the-arginylation-branch-of-the-n-end-rule-pathway-positively-regulates-cellular-autophagic-flux-and-clearance-of-proteotoxic-proteins
#11
Yanxialei Jiang, Jeeyoung Lee, Jung Hoon Lee, Joon Won Lee, Ji Hyeon Kim, Won Hoon Choi, Young Dong Yoo, Hyunjoo Cha-Molstad, Bo Yeon Kim, Yong Tae Kwon, Sue Ah Noh, Kwang Pyo Kim, Min Jae Lee
The N-terminal amino acid of a protein is an essential determinant of ubiquitination and subsequent proteasomal degradation in the N-end rule pathway. Using para-chloroamphetamine (PCA), a specific inhibitor of the arginylation branch of the pathway (Arg/N-end rule pathway), we identified that blocking the Arg/N-end rule pathway significantly impaired the fusion of autophagosomes with lysosomes. Under ER stress, ATE1-encoded Arg-tRNA-protein transferases carry out the N-terminal arginylation of the ER heat shock protein HSPA5 that initially targets cargo proteins, along with SQSTM1, to the autophagosome...
November 2016: Autophagy
https://www.readbyqxmd.com/read/27514476/soluble-bioactive-microbial-mediators-regulate-proteasomal-degradation-and-autophagy-to-protect-against-inflammation-induced-stress
#12
Yuhei Inaba, Nobuhiro Ueno, Masatsugu Numata, Xiaorong Zhu, Jeannette S Messer, David L Boone, Mikihiro Fujiya, Yutaka Kohgo, Mark W Musch, Eugene B Chang
Bifidobacterium breve and other Gram-positive gut commensal microbes protect the gastrointestinal epithelium against inflammation-induced stress. However, the mechanisms whereby these bacteria accomplish this protection are poorly understood. In this study, we examined soluble factors derived from Bifidobacterium breve and their impact on the two major protein degradation systems within intestinal epithelial cells, proteasomes and autophagy. Conditioned media from gastrointestinal Gram-positive, but not Gram-negative, bacteria activated autophagy and increased expression of the autophagy proteins Atg5 and Atg7 along with the stress response protein heat shock protein 27...
October 1, 2016: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/27507943/age-related-decrease-in-heat-shock-70-kda-protein-8-in-cerebrospinal-fluid-is-associated-with-increased-oxidative-stress
#13
David A Loeffler, Andrea C Klaver, Mary P Coffey, Jan O Aasly, Peter A LeWitt
Age-associated declines in protein homeostasis mechanisms ("proteostasis") are thought to contribute to age-related neurodegenerative disorders. The increased oxidative stress which occurs with aging can activate a key proteostatic process, chaperone-mediated autophagy. This study investigated age-related alteration in cerebrospinal fluid (CSF) concentrations of heat shock 70-kDa protein 8 (HSPA8), a molecular chaperone involved in proteostatic mechanisms including chaperone-mediated autophagy, and its associations with indicators of oxidative stress (8-hydroxy-2'-deoxyguanosine [8-OHdG] and 8-isoprostane) and total anti-oxidant capacity...
2016: Frontiers in Aging Neuroscience
https://www.readbyqxmd.com/read/27484733/melatonin-enhances-thermotolerance-by-promoting-cellular-protein-protection-in-tomato-plants
#14
Wen Xu, Shu-Yu Cai, Yun Zhang, Yu Wang, Golam Jalal Ahammed, Xiao-Jian Xia, Kai Shi, Yan-Hong Zhou, Jing-Quan Yu, Russel J Reiter, Jie Zhou
Melatonin is a pleiotropic signaling molecule that provides physiological protection against diverse environmental stresses in plants. Nonetheless, the mechanisms for melatonin-mediated thermotolerance remain largely unknown. Here we report that endogenous melatonin levels increased with a rise in ambient temperature and that peaked at 40 °C. Foliar pretreatment with an optimal dose of melatonin (10 μM) or the overexpression of N-acetylserotonin methyltransferase (ASMT) gene effectively ameliorated heat-induced photoinhibition and electrolyte leakage in tomato plants...
August 2, 2016: Journal of Pineal Research
https://www.readbyqxmd.com/read/27471925/heat-shock-proteins-in-the-context-of-photodynamic-therapy-autophagy-apoptosis-and-immunogenic-cell-death
#15
Matías E Rodríguez, Ingrid S Cogno, Laura S Milla Sanabria, Yanina S Morán, Viviana A Rivarola
Photodynamic therapy (PDT) is an anti-tumor treatment administered for the elimination of early-stage malignancies and the palliation of symptoms in patients with late-stage tumors, which involves the activation of a photosensitizer (PS) using light of a specific wavelength, which also generates singlet oxygen and other reactive oxygen species (ROS) that cause tumor cell death. Several mechanisms are involved in the protective responses to PDT including the expression of chaperone/heat shock proteins (HSPs)...
September 2016: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/27444243/%C3%AE-asarone-increases-mef2d-and-th-levels-and-reduces-%C3%AE-synuclein-level-in-6-ohda-induced-rats-via-regulating-the-hsp70-mapk-mef2d-beclin-1-pathway-chaperone-mediated-autophagy-activation-macroautophagy-inhibition-and-hsp70-up-expression
#16
Liping Huang, Minzhen Deng, Yuping He, Shiyao Lu, Shu Liu, Yongqi Fang
Inactive myocyte enhancer factor 2D (MEF2D) and alpha-synuclein (α-syn) aggregation will cause neuronal death. MEF2D or α-syn degradation is also associated with macroautophagy, chaperone-mediated autophagy (CMA) and heat-shock protein 70 (HSP70). We found that β-asarone had positive effects on treating 6-hydroxydopamine (6-OHDA)-induced rats, but mechanisms of β-asarone affecting on MEF2D and α-syn via regulating the HSP70/MAPK/MEF2D/Beclin-1 pathway remain unclear. Unilateral 6-OHDA injection into the medial forebrain bundle was used to create PD rats, which were divided into four groups and administered for 30days: 6-OHDA model group, MEF2D inhibitor-treated group (SB203580, 0...
October 15, 2016: Behavioural Brain Research
https://www.readbyqxmd.com/read/27421652/everolimus-downregulates-estrogen-receptor-and-induces-autophagy-in-aromatase-inhibitor-resistant-breast-cancer-cells
#17
Asona Lui, Jacob New, Joshua Ogony, Sufi Thomas, Joan Lewis-Wambi
BACKGROUND: mTOR inhibition of aromatase inhibitor (AI)-resistant breast cancer is currently under evaluation in the clinic. Everolimus/RAD001 (Afinitor®) has had limited efficacy as a solo agent but is projected to become part of combination therapy for AI-resistant breast cancer. This study was conducted to investigate the anti-proliferative and resistance mechanisms of everolimus in AI-resistant breast cancer cells. METHODS: In this study we utilized two AI-resistant breast cancer cell lines, MCF-7:5C and MCF-7:2A, which were clonally derived from estrogen receptor positive (ER+) MCF-7 breast cancer cells following long-term estrogen deprivation...
2016: BMC Cancer
https://www.readbyqxmd.com/read/27412471/the-nephroprotection-exerted-by-curcumin-in-maleate-induced-renal-damage-is-associated-with-decreased-mitochondrial-fission-and-autophagy
#18
Eduardo Molina-Jijón, Omar Emiliano Aparicio-Trejo, Rafael Rodríguez-Muñoz, Juan Carlos León-Contreras, María Del Carmen Cárdenas-Aguayo, Omar Noel Medina-Campos, Edilia Tapia, Laura Gabriela Sánchez-Lozada, Rogelio Hernández-Pando, José L Reyes, Laura Arreola-Mendoza, José Pedraza-Chaverri
We have previously reported that the antioxidant curcumin exerts nephroprotection in maleate-induced renal damage, a model associated with oxidative stress. However, the mechanisms involved in curcumin protective effect were not explored, to assess this issue, curcumin was administered daily by gavage (150 mg/kg) five days before a single maleate (400 mg/kg)-injection. Curcumin prevented maleate-induced proteinuria, increased heat shock protein of 72 KDa (Hsp72) expression, and decreased plasma glutathione peroxidase activity...
July 13, 2016: BioFactors
https://www.readbyqxmd.com/read/27395830/functional-inhibition-of-hsp70-by-pifithrin-%C3%AE-switches-gambogic-acid-induced-caspase-dependent-cell-death-to-caspase-independent-cell-death-in-human-bladder-cancer-cells
#19
Mohammad Ishaq, Rani Ojha, Kapil Sharma, Gaurav Sharma, Shrawan K Singh, Sekhar Majumdar
Heat shock protein-70kDa (Hsp70) is a member of molecular chaperone family, involved in the proper folding of various proteins. Hsp70 is important for tumor cell survival and is also reported to be involved in enhancing the drug resistance of various cancer types. Hsp70 controls apoptosis both upstream and downstream of the mitochondria by regulating the mitochondrial membrane permeabilization (MMP) and apoptosome formation respectively. In the present study, we have elucidated the role of Hsp70 in Gambogic acid (GA) induced apoptosis in bladder cancer cells...
November 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27381181/evidence-for-the-role-of-bag3-in-mitochondrial-quality-control-in-cardiomyocytes
#20
Farzaneh G Tahrir, Tijana Knezevic, Manish K Gupta, Jennifer Gordon, Joseph Y Cheung, Arthur M Feldman, Kamel Khalili
Mitochondrial abnormalities impact the development of myofibrillar myopathies. Therefore, understanding the mechanisms underlying the removal of dysfunctional mitochondria from cells is of great importance toward understanding the molecular events involved in the genesis of cardiomyopathy. Earlier studies have ascribed a role for BAG3 in the development of cardiomyopathy in experimental animals leading to the identification of BAG3 mutations in patients with heart failure which may play a part in the onset of disease development and progression...
July 6, 2016: Journal of Cellular Physiology
keyword
keyword
57100
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"