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Kiyoyuki Ogata, Kazuma Sei, Leonie Saft, Naoya Kawahara, Matteo G Della Porta, Nicolas Chapuis, Yumi Yamamoto
Diagnosis of myelodysplastic syndromes (MDS) is not straightforward when objective data, such as blast excess and abnormal cytogenetics, are lacking. Expert laboratories use flow cytometry (FCM) to help diagnose MDS. However, most of FCM protocols for MDS are complex, requiring a high level of expertise and high cost. We have reported a FCM mini-panel consisting of four FCM parameters (so-called Ogata score), which is simple to conduct and inexpensive. In this paper, to refine this mini-panel, we have introduced a new FCM parameter, which quantifies CD33 expression on CD34+ cells (called Granulocyte/CD34 cell CD33 ratio)...
July 10, 2018: Leukemia Research
C Kelaidi, T Braun, R Arana, A Marceau-Renaut, G Lazarian, J Soret, S Cereja, R Letestu, V Eclache, D Lusina, F Baran-Marszak, L Ades, C Preudhomme, A Martin, P Fenaux, C Gardin
Immunosuppressive treatment is a disease-modifying therapy for lower-risk myelodysplastic syndromes (MDS). However, IST is relatively rarely used and long-term outcomes of patients are seldom reported. We retrospectively studied outcomes of 20 patients with lower-risk non del 5q MDS with transfusion dependency, with horse or rabbit antithymocyte globulin ± ciclosporine A, and frontline eltrombopag in two of them. IPSS-R was low, intermediate and high in 30%, 55% and 10% of the patients, respectively. Fifty-five percent of the patients had hypocellular bone marrow (BM)...
May 29, 2018: Leukemia Research
Hyeon-Goo Kang, Yong-Soo Jeong, Yoon-Hyuk Huh, Chan-Jin Park, Lee-Ra Cho
PURPOSE: To compare the bone responses of chemically modified implants using the plasma immersion ion implantation and deposition method with those of blasted implants. MATERIALS AND METHODS: The titanium implants were blasted with resorbable blasting media (RBM) and designated as controls. The ion-implanted implants were divided into two test groups, namely, calcium (Ca) and magnesium (Mg) implants. Six implants (two implants per group) were placed into the proximal tibias of 11 New Zealand white rabbits...
July 2018: International Journal of Oral & Maxillofacial Implants
Alicia A Swan, Jeremy T Nelson, Terri K Pogoda, Megan E Amuan, Faith W Akin, Mary Jo Pugh
OBJECTIVES: To describe the prevalence of sensory dysfunction (i.e. auditory, visual, vestibular, chemosensory and multiple sensory problems) and explore associations with traumatic brain injury (TBI) severity and injury mechanism among deployed Post-9/11 Veterans. METHODS: This retrospective cohort analysis used Departments of Defense and Veterans Affairs diagnostic codes and administrative data. RESULTS: Among the 570,248 Veterans in this cohort, almost 23% had at least one diagnosis of sensory dysfunction...
July 19, 2018: Brain Injury: [BI]
Daphné Rochel, Jérôme Abadie, Cynthia Robveille, Bérengère Déqueant, Françoise Roux, Laetitia Jaillardon, Elie Dagher
This case report presents a 14-month-old female Poodle mix with acute megakaryoblastic leukemia based on a marked thrombocytosis, abnormal platelet morphology, circulating dwarf megakaryocytes, and blast cells in the blood. Bone marrow abnormalities included dysmegakaryopoiesis dygranulopoiesis, and an increased number of blast cells was observed in the blood. Extensive leukemic involvement was also found in the liver, spleen, lymph nodes, lungs, kidneys, and brain. The cytopathologic features of the abnormal circulating cells were highly suggestive of being megakaryocytic in origin, which was supported by negative myeloperoxidase staining and positive von Willebrand factor staining on immunocytochemistry (ICC)...
July 19, 2018: Veterinary Clinical Pathology
Toshiharu Takahashi, Tomoya Murano, Atsushi Ishikawa
Rice blast caused by Pyricularia oryzae (syn. Magnaporthe oryzae) is a disease devastating to rice. We have studied the Arabidopsis-P. oryzae pathosystem as a model system for nonhost resistance (NHR) and found that SOBIR1, but not BAK1, is a positive regulator of NHR to P. oryzae in Arabidopsis. AGB1 is also involved in NHR. However, the genetic interactions between SOBIR1, BAK1, and AGB1 are uncharacterized. In this study, we delineated the genetic interactions between SOBIR1, BAK1, and AGB1 in NHR to P. oryzae in Arabidopsis and found SOBIR1 and AGB1 independently control NHR to P...
July 19, 2018: Bioscience, Biotechnology, and Biochemistry
Hisayuki Yao, Trevor T Price, Gaia Cantelli, Brandon Ngo, Matthew J Warner, Lindsey Olivere, Sarah M Ridge, Elizabeth M Jablonski, Joseph Therrien, Stacey Tannheimer, Chad M McCall, Anjen Chenn, Dorothy A Sipkins
Acute lymphoblastic leukaemia (ALL) has a marked propensity to metastasize to the central nervous system (CNS). In contrast to brain metastases from solid tumours, metastases of ALL seldom involve the parenchyma but are isolated to the leptomeninges, which is an infrequent site for carcinomatous invasion. Although metastasis to the CNS occurs across all subtypes of ALL, a unifying mechanism for invasion has not yet been determined. Here we show that ALL cells in the circulation are unable to breach the blood-brain barrier in mice; instead, they migrate into the CNS along vessels that pass directly between vertebral or calvarial bone marrow and the subarachnoid space...
July 18, 2018: Nature
Tian-Hui Yang, Lisa S St John, Haven R Garber, Celine Kerros, Kathryn E Ruisaard, Karen Clise-Dwyer, Gheath Alatrash, Qing Ma, Jeffrey J Molldrem
Proteinase 3 (P3), a serine protease expressed by myeloid cells, localized within azurophil granules, and also expressed on the cellular membrane of polymorphonuclear neutrophils (PMN), is the target of autoimmunity in granulomatosis with polyangiitis. PR1, an HLA-A2 restricted nonameric peptide derived from P3, has been targeted effectively in myeloid leukemia. We previously showed (Molldrem et al. 2003. J Clin Invest 111: 639-647) that overexpression of P3 in chronic myeloid leukemia induces apoptosis of high-affinity PR1-specific T cells, leading to deletional tolerance and leukemia outgrowth...
July 18, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
Kazunori D Yamada, Kengo Kinoshita
BACKGROUND: Long short-term memory (LSTM) is one of the most attractive deep learning methods to learn time series or contexts of input data. Increasing studies, including biological sequence analyses in bioinformatics, utilize this architecture. Amino acid sequence profiles are widely used for bioinformatics studies, such as sequence similarity searches, multiple alignments, and evolutionary analyses. Currently, many biological sequences are becoming available, and the rapidly increasing amount of sequence data emphasizes the importance of scalable generators of amino acid sequence profiles...
July 18, 2018: BMC Bioinformatics
Christine Marizzi, Antonia Florio, Melissa Lee, Mohammed Khalfan, Cornel Ghiban, Bruce Nash, Jenna Dorey, Sean McKenzie, Christine Mazza, Fabiana Cellini, Carlo Baria, Ron Bepat, Lena Cosentino, Alexander Dvorak, Amina Gacevic, Cristina Guzman-Moumtzis, Francesca Heller, Nicholas Alexander Holt, Jeffrey Horenstein, Vincent Joralemon, Manveer Kaur, Tanveer Kaur, Armani Khan, Jessica Kuppan, Scott Laverty, Camila Lock, Marianne Pena, Ilona Petrychyn, Indu Puthenkalam, Daval Ram, Arlene Ramos, Noelle Scoca, Rachel Sin, Izabel Gonzalez, Akansha Thakur, Husan Usmanov, Karen Han, Andy Wu, Tiger Zhu, David Andrew Micklos
DNA barcoding is both an important research and science education tool. The technique allows for quick and accurate species identification using only minimal amounts of tissue samples taken from any organism at any developmental phase. DNA barcoding has many practical applications including furthering the study of taxonomy and monitoring biodiversity. In addition to these uses, DNA barcoding is a powerful tool to empower, engage, and educate students in the scientific method while conducting productive and creative research...
2018: PloS One
Fei Chen, Jiawei Zhang, Junhao Chen, Xiaojiang Li, Wei Dong, Jian Hu, Meigui Lin, Yanhui Liu, Guowei Li, Zhengjia Wang, Liangsheng Zhang
With over 6000 species in seven classes, red algae (Rhodophyta) have diverse economic, ecological, experimental and evolutionary values. However, red algae are usually absent or rare in comparative analyses because genomic information of this phylum is often under-represented in various comprehensive genome databases. To improve the accessibility to the ome data and omics tools for red algae, we provided 10 genomes and 27 transcriptomes representing all seven classes of Rhodophyta. Three genomes and 18 transcriptomes were de novo assembled and annotated in this project...
January 1, 2018: Database: the Journal of Biological Databases and Curation
Zhang Lu-Fei, Wang Ling-Jun, Zheng Ming-Hui, Cao Jian-Ping, Liu Hui
OBJECTIVE: To investigate the molecular characteristics of genome sequence of Thelazia callipaeda (T. cp) . METHODS: The obtained T. cp genome assembling data were annotated by using a combination of ab initio gene by softwares, GeneMark and GeneID, and the homology of the experimentally confirmed genes was predicted by software GeMoMa. The results were integrated by software EVM to predict all genes of genome. The obtained genes were annotated in the common public database and three dedicated databases (CAZyme, TCDB and PHI), respectively...
March 26, 2018: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
Peter Valent, Hans-Peter Horny, Michel Arock
Chronic myeloid leukemia (CML) is a hematopoietic neoplasm defined by the chromosome translocation t(9;22) and the related oncogene, BCR-ABL1. In most patients, leukemic cells can be kept under control using BCR-ABL1-targeting drugs. However, many patients relapse which remains a clinical challenge. Especially patients with advanced (accelerated or blast phase) CML have a poor prognosis. So far, little is known about molecular and cellular interactions and features that contribute to disease progression and drug resistance in CML...
July 17, 2018: European Journal of Clinical Investigation
Françoise Arnaud, Georgina Pappas, Ye Chen, Eric Maudlin-Jeronimo, Richard McCarron
INTRODUCTION: A stressful environment may contribute to poor outcomes after TBI. The current study evaluates the impact of acute stress in a polytrauma rat model. METHODS: Rats were stressed by a 45-minute immobilization period before instrumentation under ketamine (t1). Polytrauma was produced by blast overpressure and controlled hemorrhage (t2). Rats were euthanized immediately after a 3 h simulated Medevac-transport time (t3) or after 72 h post-trauma (t4)...
July 6, 2018: Neuroscience Letters
Suniah S Ayub, Saleem Islam, Cynthia D Downard, Adam B Goldin, David M Powell, Janice A Taylor
PURPOSE: American Pediatric Surgical Association (APSA) committees send monthly email blasts to members with links to peer-reviewed publications. This study assesses the utilization of this service. METHODS: Email-opening and click-through rates were analyzed from 5/2012 to 4/2017 and compared to APSA and industry standards. Access was analyzed based on subject, disease type, journal, impact factor, and committee. CME questions were added in 10/2014 and emails consolidated in 1/2016...
June 22, 2018: Journal of Pediatric Surgery
Dalrene Teresa Keerthika James, Augustine Osamor Ifelebuegu
Grey water reclamation is a sustainable solution for managing water shortages. However, grey water contains high levels of detergents, particularly surfactants, which alongside other chemical constituents can pose a serious risk to human health and the environment. Biological treatments are not effective in the treatment of grey water since the detergents inhibit the activities of microorganisms. Chemical treatment options have an impact on public perception and could be cost prohibitive for domestic and small scale reuse applications...
July 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
Muhammad Aslam, Faiz-Ul-Hassan Nasim, Rana Ruhi, Hassan Murad, Samina Ejaz, Muhammad Shafiq Choudhary, Ghulam Mustafa, Muhammad Ashraf, Jameel Rehman
Rhizobacteria are an active part of microbial population in the rhizosphere of plants. In this study, twenty rhizobacteria were isolated from the rhizosphere of a perennial grass, Haloxylon salicornicum, found in Cholistan desert, an arid landmass near Bahawalpur Pakistan, in one set of experimental conditions. Colony characteristics, biochemical and molecular analyses of these isolates were performed. All isolates were bacilli, gram positive with off-white colonies and exhibited typical bacilli colony morphology...
March 9, 2018: Polish Journal of Microbiology
Sören Hansen, Oumar Faye, Sabri S Sanabani, Martin Faye, Susanne Böhlken-Fascher, Ousmane Faye, Amadou A Sall, Michaël Bekaert, Manfred Weidmann, Claus-Peter Czerny, Ahmed Abd El Wahed
BACKGROUND: Outbreaks of fever of unknown origin start with nonspecific symptoms and case definition is only slowly developed and adapted, therefore, identifying the causative agent is crucial to ensure suitable treatment and control measures. As an alternative method for Polymerase Chain Reaction in molecular diagnostics diagnostic, metagenomics can be applied to identify the pathogen responsible for the outbreak through sequencing all nucleic acids present in a sample extract. Sequencing data obtained can identify new or variants of known agents...
July 6, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
T T Hickman, C Smalt, J Bobrow, T Quatieri, M C Liberman
When exposed to continuous high-level noise, cochlear neurons are more susceptible to damage than hair cells (HCs): exposures causing temporary threshold shifts (TTS) without permanent HC damage can destroy ribbon synapses, permanently silencing the cochlear neurons they formerly activated. While this "hidden hearing loss" has little effect on thresholds in quiet, the neural degeneration degrades hearing in noise and may be an important elicitor of tinnitus. Similar sensory pathologies are seen after blast injury, even if permanent threshold shift (PTS) is minimal...
July 16, 2018: Scientific Reports
Robert Gerhard Stigler, Kathrin Becker, Michela Bruschi, Doris Steinmüller-Nethl, Robert Gassner
By coating surfaces with nano-crystalline diamond (NCD) particles, hydrophilicity can be altered via sidechain modifications without affecting surface texture. The present study aimed to assess the impact of NCD hydrophilicity on machined and rough SLA titanium discs on soft tissue integration, using a rodent model simulating submerged healing. Four different titanium discs (machined titanium = M Titanium, NCD-coated hydrophilic machined titanium = M-O-NCD, sand blasted acid etched (SLA Titanium) titanium, and hydrophilic NCD-coated SLA titanium = SLA O-NCD) were inserted in subdermal pockets of 12 Wistar rats...
July 13, 2018: Nanomaterials
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