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https://www.readbyqxmd.com/read/28918151/internal-transcribed-spacer-its-sequencing-reveals-considerable-fungal-diversity-in-dairy-products
#1
A J Buehler, R L Evanowski, N H Martin, K J Boor, M Wiedmann
Fungi are important spoilage organisms in dairy products. However, little is known about the diversity of naturally occurring spoilage fungi in raw milk and processed dairy products, due at least in part to the fact that classical fungal identification methods require considerable expertise. To gain further insight into the fungal diversity in the dairy system, we isolated fungi from raw milk, raw and pasteurized milk cheese, and yogurt using the selective dichloran rose bengal chloramphenicol agar. In total, 361 fungal isolates were obtained and further characterized by DNA sequencing of the internal transcribed spacer (ITS) region and the nuclear ribosomal large subunit (LSU) rRNA gene if needed...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28917710/molecular-identification-of-vermamoeba-vermiformis-from-freshwater-fish-in-lake-taal-philippines
#2
Giovanni D Milanez, Frederick R Masangkay, Rey C Thomas, Ma Olive Grace O Ordona, Gabriel Q Bernales, Vyanna Camille M Corpuz, Hannah Selina V Fortes, Charezze Margarette S Garcia, Larra Camille Nicolas, Veeranoot Nissapatorn
Free Living Amoebae (FLA) are considered ubiquitous. FLAs may infect various biological organisms which act as reservoir hosts. Infected freshwater fishes can pose a public health concern due to possible human consumption. This study aims to identify possible pathogenic FLAs present in freshwater fishes. Seventy five (75) Oreochromis niloticus were studied for the presence of FLAs. Fish organs were suspended in physiologic saline pelleted and cultured in non-nutrient agar (NNA) lawned with Escherichia coli and were incubated in 33 °C for 14 days...
September 13, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28912793/diverse-rice-landraces-of-north-east-india-enables-the-identification-of-novel-genetic-resources-for-magnaporthe-resistance
#3
Bangale Umakanth, Balija Vishalakshi, P Sathish Kumar, S J S Rama Devi, Vijay Pal Bhadana, P Senguttuvel, Sudhir Kumar, Susheel Kumar Sharma, Pawan Kumar Sharma, M S Prasad, Maganti S Madhav
North-East (NE) India, the probable origin of rice has diverse genetic resources. Many rice landraces of NE India were not yet characterized for blast resistance. A set of 232 landraces of NE India, were screened for field resistance at two different hotspots of rice blast, viz., IIRR-UBN, Hyderabad and ICAR-NEH, Manipur in two consecutive seasons. The phenotypic evaluation as well as gene profiling for 12 major blast resistance genes (Pitp, Pi33, Pi54, Pib, Pi20, Pi38, Pita2, Pi1, Piz, Pi9, Pizt, and Pi40) with linked as well as gene-specific markers, identified 84 resistant landraces possessing different gene(s) either in singly or in combinations and also identified seven resistant landraces which do not have the tested genes, indicating the valuable genetic resources for blast resistance...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28912174/senescence-is-a-spi1-pu-1-induced-anti-proliferative-mechanism-in-primary-hematopoietic-cells
#4
Laure Delestré, Cui Hengxiang, Michela Esposito, Cyril Quiveron, Elena Mylonas, Virginie Penard-Lacronique, Oliver Bischof, Christel Guillouf
Transcriptional deregulation caused by epigenetic or genetic alterations is a major cause of leukemic transformation. The Spi1/PU.1 transcription factor is a key regulator of many steps of hematopoiesis, and limits self-renewal of hematopoietic stem cells. The deregulation of its expression or activity contributes to leukemia, in which Spi1 can be either an oncogene or a tumor suppressor. Here, we explored whether cellular senescence, an anti-tumoral pathway that restrains cell proliferation, is a mechanism by which Spi1 limits hematopoietic cells expansion, and thus prevents the development of leukemia...
September 14, 2017: Haematologica
https://www.readbyqxmd.com/read/28911735/antifungal-activity-of-sterols-and-dipsacus-saponins-isolated-from-dipsacus-asper-roots-against-phytopathogenic-fungi
#5
Nam Hee Choi, Ja Yeong Jang, Gyung Ja Choi, Yong Ho Choi, Kyoung Soo Jang, Van Thu Nguyen, Byung-Sun Min, Quang Le Dang, Jin-Cheol Kim
The in vivo antifungal activity of crude extracts of Dipsacus asper roots was evaluated against the phytopathogenic fungi Botrytis cinerea, Colletotrichum coccodes, Blumeria graminis f. sp. hordei, Magnaporthe grisea, Phytophthora infestans, Puccinia recondita and Rhizoctonia solani using a whole-plant assay method. Ethyl acetate and acetone extracts, at 1000μg/mL, suppressed the development of tomato gray mold (TGM) and tomato late blight (TLB) by 90%. Through bioassay-guided isolation, five antifungal substances were isolated from the D...
September 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/28911333/profiling-bacterial-communities-by-minion-sequencing-of-ribosomal-operons
#6
Lee J Kerkhof, Kevin P Dillon, Max M Häggblom, Lora R McGuinness
BACKGROUND: An approach utilizing the long-read capability of the Oxford Nanopore MinION to rapidly sequence bacterial ribosomal operons of complex natural communities was developed. Microbial fingerprinting employs domain-specific forward primers (16S rRNA subunit), reverse primers (23S rRNA subunit), and a high-fidelity Taq polymerase with proofreading capabilities. Amplicons contained both ribosomal subunits for broad-based phylogenetic assignment (~ 3900 bp of sequence), plus the intergenic spacer (ITS) region (~ 300 bp) for potential strain-specific identification...
September 15, 2017: Microbiome
https://www.readbyqxmd.com/read/28906207/high-absolute-basophil-count-is-a-powerful-independent-predictor-of-inferior-overall-survival-in-patients-with-primary-myelofibrosis
#7
Marko Lucijanic, Ana Livun, Tajana Stoos-Veic, Vlatko Pejsa, Ozren Jaksic, David Cicic, Jelena Lucijanic, Zeljko Romic, Biserka Orehovec, Gorana Aralica, Marko Miletic, Rajko Kusec
OBJECTIVES: To investigate the clinical and prognostic significance of absolute basophil count (ABC) in patients with primary myelofibrosis (PMF). METHODS: We retrospectively investigated 58 patients with PMF treated in our institution in the period from 2006 to 2017. ABC was obtained in addition to other hematological and clinical parameters. Patients were separated into high and low ABC groups using the Receiver operating characteristic curve analysis. RESULTS: ABC was higher in PMF patients than in healthy controls (P < 0...
September 14, 2017: Hematology (Amsterdam, Netherlands)
https://www.readbyqxmd.com/read/28905253/co-transformation-mediated-stacking-of-blast-resistance-genes-pi54-and-pi54rh-in-rice-provides-broad-spectrum-resistance-against-magnaporthe-oryzae
#8
Mandeep Kumari, Amit Kumar Rai, B N Devanna, Pankaj Kumar Singh, Ritu Kapoor, H Rajashekara, G Prakash, Vinay Sharma, Tilak Raj Sharma
This is the first report of stacking two major blast resistance genes in blast susceptible rice variety using co-transformation method to widen the resistance spectrum against different isolates of Magnaporthe oryzae. Single resistance (R-) gene mediated approach for the management of rice blast disease has met with frequent breakdown in resistance response. Besides providing the durable resistance, gene pyramiding or stacking also imparts broad spectrum resistance against plant pathogens, including rice blast...
September 13, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28903910/genome-wide-identification-subcellular-localization-and-gene-expression-analysis-of-the-members-of-cesa-gene-family-in-common-tobacco-nicotiana-tabacum-l
#9
Zong-Chang Xu, Yingzhen Kong
Cellulose-synthase proteins (CESAs) are membrane localized proteins and they form protein complexes to produce cellulose in the plasma membrane. CESA proteins play very important roles in cell wall construction during plant growth and development. In this study, a total of 21 NtCESA gene sequences were identified by using PF03552 conserved protein sequence and 10 AtCESA protein sequences of Arabidopsis thaliana to blast against the common tobacco (Nicotiana tabacum L.) genome database with TBLASTN protocol...
June 20, 2017: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/28902862/distinct-genetic-clades-of-enterovirus-d68-detected-in-2010-2013-and-2015-in-osaka-city-japan
#10
Atsushi Kaida, Nobuhiro Iritani, Seiji P Yamamoto, Daiki Kanbayashi, Yuki Hirai, Masao Togawa, Kiyoko Amo, Urara Kohdera, Toshinori Nishigaki, Masashi Shiomi, Sadasaburo Asai, Tsutomu Kageyama, Hideyuki Kubo
The first upsurge of enterovirus D68 (EV-D68), a causative agent of acute respiratory infections (ARIs), in Japan was reported in Osaka City in 2010. In this study, which began in 2010, we surveyed EV-D68 in children with ARIs and analyzed sequences of EV-D68 strains detected. Real-time PCR of 19 respiratory viruses or subtypes of viruses, including enterovirus, was performed on 2,215 specimens from ARI patients (<10 years of age) collected between November 2010 and December 2015 in Osaka City, Japan. EV-D68 was identified in 18 enterovirus-positive specimens (n = 4 in 2013, n = 1 in 2014, and n = 13 in 2015) by analysis of viral protein 1 (VP1) or VP4 sequences, followed by a BLAST search for similar sequences...
2017: PloS One
https://www.readbyqxmd.com/read/28902395/finding-homologs-in-amino-acid-sequences-using-network-blast-searches
#11
Istvan Ladunga
BLAST, the Basic Local Alignment Search Tool, is used more frequently than any other biosequence database search program. We show how to run searches on the Web, and demonstrate how to increase performance by fine-tuning arguments for a specific research project. We offer guidance for interpreting results, statistical significance and biological relevance issues, and suggest complementary analyses. This unit covers both protein-to-protein (blastp) searches and translated searches (blastx, tblastn, tfastx). blastx conceptually translates the query sequence and tblastn translates all nucleotide sequences in a database, while tblastx translates both the query and the database sequences into amino acid sequences...
September 13, 2017: Current Protocols in Bioinformatics
https://www.readbyqxmd.com/read/28901858/expression-of-a-nitric-oxide-synthesizing-protein-in-arterial-endothelial-cells-in-response-to-different-anti-anginal-agents-used-in-acute-coronary-syndromes
#12
Sarbashri Bank, Pradipta Jana, G V Girish, Asru Sinha, Smarajit Maiti
Organic "nitro" compounds such as nitroglycerine, isosorbate nitrate are useful in the control of chest pain in acute coronary syndrome. But the mechanism of it in pain regulation remains speculative. Here, increase of NO production was investigated by the possible regulation of constitutive nitric oxide synthase (cNOS) function from goat arterial endothelial cells. This protein was purified and sequence wise characterized as protein disulfide isomerase (PDI) in response to different nitro compounds. The NO generating protein was isolated from arterial endothelial cells and prepared to homogeneity...
September 11, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28901019/isolation-and-characterization-of-pathogenic-vibrio-alginolyticus-from-sea-cage-cultured-cobia-rachycentron-canadum-linnaeus-1766-%C3%A2-in-india
#13
P Rameshkumar, A K A Nazar, M A Pradeep, C Kalidas, R Jayakumar, G Tamilmani, M Sakthivel, A K Samal, S Sirajudeen, V Venkatesan, B M Nazeera
Mass mortalities of cobia Rachycentron canadum sub-adults occurred during August 2013 in cage culture at the Gulf of Mannar, Mandapam Tamil Nadu, India. The epizootic of disease was started with typical classical clinical signs followed by the acute mortality. Grossly, severe haemorrhage and congestion was observed in the gastric mucosa. Abdomen was distended with peritoneal fluid. Heart revealed haemopericardium and fibrinous pericardium. Histologically, the gastric mucosa showed severe erosion and necrosis...
September 12, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28900603/a-nanocomplex-of-c60-fullerene-with-cisplatin-design-characterization-and-toxicity
#14
Svitlana Prylutska, Svitlana Politenkova, Kateryna Afanasieva, Volodymyr Korolovych, Kateryna Bogutska, Andriy Sivolob, Larysa Skivka, Maxim Evstigneev, Viktor Kostjukov, Yuriy Prylutskyy, Uwe Ritter
The self-organization of C60 fullerene and cisplatin in aqueous solution was investigated using the computer simulation, dynamic light scattering and atomic force microscopy techniques. The results evidence the complexation between the two compounds. The genotoxicity of С60 fullerene, Cis and their complex was evaluated in vitro with the comet assay using human resting lymphocytes and lymphocytes after blast transformation. The cytotoxicity of the mentioned compounds was estimated by Annexin V/PI double staining followed by flow cytometry...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28900205/apoe4-associated-phospholipid-dysregulation-contributes-to-development-of-tau-hyper-phosphorylation-after-traumatic-brain-injury
#15
Jiqing Cao, Farida El Gaamouch, James S Meabon, Kole D Meeker, Li Zhu, Margaret B Zhong, John Bendik, Gregory Elder, Ping Jing, Jiahong Xia, Wenjie Luo, David G Cook, Dongming Cai
The apolipoprotein E4 (ApoE4) genotype combines with traumatic brain injury (TBI) to increase the risk of developing Alzheimer's Disease (AD). However, the underlying mechanism(s) is not well-understood. We found that after exposure to repetitive blast-induced TBI, phosphoinositol biphosphate (PIP2) levels in hippocampal regions of young ApoE3 mice were elevated and associated with reduction in expression of a PIP2 degrading enzyme, synaptojanin 1 (synj1). In contrast, hippocampal PIP2 levels in ApoE4 mice did not increase after blast TBI...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28899748/biomonitoring-of-2-4-6-trinitrotoluene-and-degradation-products-in-the-marine-environment-with-transplanted-blue-mussels-m-edulis
#16
Jennifer S Strehse, Daniel Appel, Catharina Geist, Hans-Jörg Martin, Edmund Maser
Since World War I considerable amounts of warfare material have been dumped at sea worldwide, but little is known about the fate of the explosive components in the marine environment. Sea dumped munitions are able to contaminate the surroundings because of the release of explosive chemicals due to corrosion and breaching or by detonation after blast-operations. This implies the risk of accumulation of toxic compounds in human and wildlife food chains. With the help of divers, we performed an active biomonitoring study with transplanted blue mussels (M...
September 9, 2017: Toxicology
https://www.readbyqxmd.com/read/28893848/a-review-of-10-years-of-scapula-injuries-sustained-by-uk-military-personnel-on-operations
#17
Darren C Roberts, D M Power, S A Stapley
BACKGROUND: Scapula fractures are relatively uncommon injuries, mostly occurring due to the effects of high-energy trauma. Rates of scapula fractures are unknown in the military setting. The aim of this study is to analyse the incidence, aetiology, associated injuries, treatment and complications of these fractures occurring in deployed military personnel. METHODS: All UK military personnel returning with upper limb injuries from Afghanistan and Iraq were retrospectively reviewed using the Royal Centre for Defence Medicine database and case notes (2004-2014)...
September 11, 2017: Journal of the Royal Army Medical Corps
https://www.readbyqxmd.com/read/28893643/neuropeptide-discovery-in-proasellus-cavaticus-prediction-of-the-first-large-scale-peptidome-for-a-member-of-the-isopoda-using-a-publicly-accessible-transcriptome
#18
Andrew E Christie
In silico transcriptome mining is one of the most effective methods for neuropeptide discovery in crustaceans, particularly for species that are small, rare or from geographically inaccessible habitats that make obtaining the large pools of tissue needed for other peptide discovery platforms impractical. Via this approach, large peptidomes have recently been described for members of many of the higher crustacean taxa, one notable exception being the Isopoda; no peptidome has been predicted for any member of this malacostracan order...
September 8, 2017: Peptides
https://www.readbyqxmd.com/read/28892915/myeloid-sarcoma-presenting-as-nasal-and-orbital-mass-an-initial-manifestation-of-an-acute-myeloid-leukaemia
#19
Amita Jain Gupta, Shramana Mandal, Richa Gupta, Nita Khurana, Achal Gulati
Myeloid sarcoma is an extramedullary manifestation of Acute Myeloid Leukaemia and sometimes is the only indicator of the disease. The incidence varies between 3-9.1% of acute leukaemia cases. The blast infiltration is seen most commonly in skin, lymph node, gastrointestinal tract, bone, soft tissue though can involve any body site usually as a solitary lesion and is rarely seen in nasal cavity. We present two cases of myeloid sarcoma presenting as a nasal mass in a six year old girl and other as orbital mass in 32-year-old as an initial manifestation of acute myeloid leukaemia...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28892661/inhibition-of-c-rel-using-sirna-increased-apoptosis-and-decreased-proliferation-in-pre-b-all-blasts-therapeutic-implications
#20
Seyedeh Momeneh Mohammadi, Daryosh Mohammadnejad, Abbas Ali Hosseinpour Feizi, Ali Akbar Movassaghpour, Soheila Montazersaheb, Hojjatollah Nozad Charoudeh
The c-Rel transcription factor is a unique member of the NF-kB family that has a role in apoptosis, proliferation and cell survival. Overexpression of c-Rel is detected in many human B cell tumors, including B-cell leukemia and several cancers. The study aimed to investigate the effects of c-Rel siRNA on the proliferation and apoptosis of relapsed pre-B acute leukemia cells. The c-Rel siRNA was transfected into Leukemia cells using an Amaxa cell line Nucleofector kit L (Lonza). Quantitative real-time RT-PCR (qRT-PCR) and western blot were done to measure the expression levels of mRNA and protein, respectively...
August 26, 2017: Leukemia Research
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