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https://www.readbyqxmd.com/read/29786466/mineralo-organic-nanoparticles-in-health-and-disease-an-overview-of-recent-findings
#1
Jan Martel, Cheng-Yeu Wu, Hsin-Hsin Peng, John D Young
We observed earlier that mineralo-organic nanoparticles form in human body fluids when the concentrations of calcium, carbonate and phosphate exceed saturation. The particles have been shown to represent mineral precursors in developing bones and teeth as well as in ectopic calcification and kidney stones. Recent studies suggest that the mineral particles may also be involved in other physiological processes, including immune tolerance against the gut microbiota and food antigens. We review here the involvement of mineralo-organic nanoparticles in physiological and pathological processes and discuss recent findings that reveal novel and unexpected roles for these particles in the human body...
May 22, 2018: Nanomedicine
https://www.readbyqxmd.com/read/29782142/projection-based-3d-printing-of-cell-patterning-scaffolds-with-multi-scale-channels
#2
Dai Xue, Yancheng Wang, Jiaxin Zhang, Deqing Mei, Yue Wang, Shaochen Chen
To fully actualize artificial, cell-laden biological models in tissue engineering, such as 3D organoids and organs-on-a-chip systems, cells need to be patterned such that they can precisely mimic natural microenvironments in vitro. Despite increasing interest in this area, patterning cells at multiscales (~10 μm - 10 mm) remains a significant challenge in bioengineering. Here we report a projection-based 3D printing system that achieves rapid and high-resolution fabrication of hydrogel scaffolds featuring intricate channels for multi-scale cell patterning...
May 21, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29779495/closing-the-phosphorus-cycle-in-a-food-system-insights-from-a-modelling-exercise
#3
H R J van Kernebeek, S J Oosting, M K van Ittersum, R Ripoll-Bosch, I J M de Boer
Mineral phosphorus (P) used to fertilise crops is derived from phosphate rock, which is a finite resource. Preventing and recycling mineral P waste in the food system, therefore, are essential to sustain future food security and long-term availability of mineral P. The aim of our modelling exercise was to assess the potential of preventing and recycling P waste in a food system, in order to reduce the dependency on phosphate rock. To this end, we modelled a hypothetical food system designed to produce sufficient food for a fixed population with a minimum input requirement of mineral P...
May 21, 2018: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29773003/antioxidant-action-of-an-aqueous-extract-of-royal-sun-medicinal-mushroom-agaricus-brasiliensis-agaricomycetes-in-rats-with-adjuvant-induced-arthritis
#4
Aline Cristine da Silva de Souza, Geferson de Almeida Gonсalves, Andréia Assunсão Soares, Anacharis B de Sá-Nakanishi, Ana Paula de Santi-Rampazzo, Maria Raquel Marсal Natali, Rosane Marina Peralta, Adelar Bracht
The purpose of this study was to evaluate possible effects of the administration of an aqueous Agaricus brasiliensis extract on the oxidative state of the liver, brain, and plasma in adjuvant-induced arthritic rats, a model for human rheumatoid arthritis. Daily doses of 400 mg · kg-1 were administered by gavage for 23 days. This treatment produced significant diminutions in protein carbonylation and lipid damage in the liver, brain, and plasma. It also diminished the tissue reactive oxygen species and increased the antioxidant capacity of the plasma...
2018: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/29769498/quantitative-analysis-of-nucleic-acid-extraction-methods-for-vibrio-cholerae-using-real-time-pcr-and-conventional-pcr
#5
Z Z Hossain, J Ferdous, S M Tulsiani, P M Jensen, A Begum
The devastating diarrheal disease cholera is caused by the bacterium Vibrio cholerae. Nucleic acid extraction is the primary step for several molecular detection approaches. In order to identify the sources of cholera illness, an efficient, fast and easy DNA extraction method for toxigenic Vibrio cholerae is desired which can be applied on diverse type of samples. This methodology developmental setup study was performed in the Environmental Microbiology Lab, Department of Microbiology, University of Dhaka, Bangladesh from March 2015 to May 2015 attempted to compare three DNA extraction methods for efficient detection and quantification of Vibrio cholerae...
April 2018: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/29764335/comparative-bioaccumulation-and-effects-of-purified-and-cellular-extract-of-cylindrospermopsin-to-freshwater-fish-hoplias-malabaricus
#6
Rodrigo de Cássio da Silva, Sonia Regina Grötzner, Daniele Dietrich Moura Costa, Juan Ramón Esquivel Garcia, Juan Muelbert, Valéria Freitas de Magalhães, Francisco Filipak Neto, Ciro Alberto de Oliveira Ribeiro
Many tropical freshwater ecosystems are impacted by cyanobacteria blooms increasing the risk of cyanotoxins exposure to aquatic organisms while human populations may be exposed by eating fish, drinking water, or dermal swimming. However, few toxicological data are available on the influence of cyanobacteria blooms in particular, cylindrospermopsin (CYN) on Brazilian neotropical fish. A number of studies demonstrated the ability of CYN to bioaccumulate in freshwater organisms and consequently enter the human food chain...
May 15, 2018: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/29760112/macronutrient-and-carbon-supply-uptake-and-cycling-across-the-antarctic-peninsula-shelf-during-summer
#7
Sian F Henley, Elizabeth M Jones, Hugh J Venables, Michael P Meredith, Yvonne L Firing, Ribanna Dittrich, Sabrina Heiser, Jacqueline Stefels, Julie Dougans
The West Antarctic Peninsula shelf is a region of high seasonal primary production which supports a large and productive food web, where macronutrients and inorganic carbon are sourced primarily from intrusions of warm saline Circumpolar Deep Water. We examined the cross-shelf modification of this water mass during mid-summer 2015 to understand the supply of nutrients and carbon to the productive surface ocean, and their subsequent uptake and cycling. We show that nitrate, phosphate, silicic acid and inorganic carbon are progressively enriched in subsurface waters across the shelf, contrary to cross-shelf reductions in heat, salinity and density...
June 28, 2018: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
https://www.readbyqxmd.com/read/29757407/transcriptome-and-metabolome-analysis-provide-insights-into-root-and-root-released-organic-anion-responses-to-phosphorus-deficiency-in-oat
#8
Yanliang Wang, Erik Lysøe, Tegan Armarego-Marriott, Alexander Erban, Lisa Paruch, Andre van Eerde, Ralph Bock, Jihong Liu-Clarke
Root and root-released organic anions play important roles in uptake of phosphorus (P), an essential macronutrient for food production. Oat, ranking sixth in the world's cereal production, contains valuable nutritional compounds and can withstand poor soil conditions. Our aim was to investigate root transcriptional and metabolic responses of oat grown under P-deficient and P-sufficient conditions. We conducted a hydroponic experiment and measured root morphology and organic anion exudation, and analysed changes in the transcriptome and metabolome...
May 11, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29756545/in-vitro-and-in-vivo-correlation-of-bmp-2-release-profiles-from-complex-delivery-vehicles
#9
Maurits Geert Laurent Olthof, Marianna A Tryfonidou, Mahrokh Dadsetan, Wouter Dhert, Michael J Yaszemski, Diederik H R Kempen, Lichun Lu
Local sustained delivery of bioactive molecules from biomaterials is a promising strategy to enhance bone regeneration. To optimize delivery vehicles for bone formation, the design characteristics are tailored with consequential effect on BMP-2 release and bone regeneration. Complying with the 3R principles, the growth factor release is often investigated in vitro using several buffers to mimic the in vivo physiological environment. However, this remains an unmet need. Therefore, this study investigates the correlation between the in vitro and in vivo (IVIVC) BMP-2 release from complex delivery vehicles in several commonly used in vitro buffers: cell culture model, phosphate buffered saline, and a strong desorption buffer...
May 14, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29738229/arginine-containing-surfactant-like-peptides-interaction-with-lipid-membranes-and-antimicrobial-activity
#10
Valeria Castelletto, Ruth H Barnes, Kimon-Andreas Karatzas, Charlotte J C Edwards-Gayle, Francesca Greco, Ian W Hamley, Robert Rambo, Jani Seitsonen, Janne Ruokolainen
The activity of antimicrobial peptides stems from their interaction with bacterial membranes, which are disrupted according to a number of proposed mechanisms. Here, we investigate the interaction of a model antimicrobial peptide that contains a single arginine residue with vesicles containing model lipid membranes. The surfactant-like peptide Ala6-Arg (A6R) is studied in the form where both termini are capped (CONH-A6R-NH2, capA6R) or uncapped (NH2-A6R-OH, A6R). Lipid membranes are selected to correspond to model anionic membranes (POPE/POPG) resembling those in bacteria or model zwitterionic membranes (POPC/DOPC) similar to those found in mammalian cells...
May 8, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29736189/investigation-of-the-influence-of-high-glucose-on-molecular-and-genetic-responses-an-in-vitro-study-using-a-human-intestine-model
#11
Tugce Boztepe, Sukru Gulec
Background: Dietary glucose consumption has increased worldwide. Long-term high glucose intake contributes to the development of obesity and type 2 diabetes mellitus (T2DM). Obese people tend to eat glucose-containing foods, which can lead to an addiction to glucose, increased glucose levels in the blood and intestine lumen, and exposure of intestinal enterocytes to high dietary glucose. Recent studies have documented a role for enterocytes in glucose sensing. However, the molecular and genetic relationship between high glucose levels and intestinal enterocytes has not been determined...
2018: Genes & Nutrition
https://www.readbyqxmd.com/read/29731236/attention-to-food-phosphate-and-nutrition-labeling
#12
Marcela T Watanabe, Pasqual Barretti, Jacqueline C T Caramori
No abstract text is available yet for this article.
May 3, 2018: Journal of Renal Nutrition
https://www.readbyqxmd.com/read/29728034/-development-of-indel-markers-for-molecular-authentication-of-panax-ginseng-and-p-quinquefolius
#13
Rong-Bo Wang, Hui-Li Tian, Hong-Tao Wang, Gui-Sheng Li
Panax ginseng and P. quinquefolius are two kinds of important medicinal herbs. They are morphologically similar but have different pharmacological effects. Therefore, botanical origin authentication of these two ginsengs is of great importance for ensuring pharmaceutical efficacy and food safety. Based on the fact that intron position in orthologous genes is highly conserved across plant species, intron length polymorphisms were exploited from unigenes of ginseng. Specific primers were respectively designed for these two species based on their insertion/deletion sequences of cytochrome P450 and glyceraldehyde 3-phosphate dehydrogenase, and multiplex PCR was conducted for molecular authentication of P...
April 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29720836/identification-of-potential-anticancer-protein-targets-in-cytotoxicity-mediated-by-tropical-medicinal-fern-extracts
#14
Siok-Thing Tan, Hean-Chooi Ong, Tsun-Thai Chai, Fai-Chu Wong
Background: Medicinal fern species represent a potentially important source for both food and medicinal applications. Previously, two underutilized tropical fern species (Blechnum orientale and Phymatopteris triloba) were reported with cytotoxic activities against selected cancer cell lines. However, the exact mechanism remains elusive. Objective: In this paper, we reported the identification of six differentially expressed proteins isolated from cancer cells, following exposure to the cytotoxic fern extracts...
April 2018: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29712558/enhancement-of-the-catalytic-activity-of-isopentenyl-diphosphate-isomerase-idi-from-saccharomyces-cerevisiae-through-random-and-site-directed-mutagenesis
#15
Hailin Chen, Meijie Li, Changqing Liu, Haibo Zhang, Mo Xian, Huizhou Liu
BACKGROUND: Lycopene is a terpenoid pigment that has diverse applications in the food and medicine industries. A prospective approach for lycopene production is by metabolic engineering in microbial hosts, such as Escherichia coli. Isopentenyl diphosphate isomerase (IDI, E.C. 5.3.3.2) is one of the rate-limiting enzymes in the lycopene biosynthetic pathway and one major target during metabolic engineering. The properties of IDIs differ depending on the sources, but under physiological conditions, IDIs are limited by low enzyme activity, short half-life and weak substrate affinity...
April 30, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29709309/a-systematic-review-on-the-composting-of-green-waste-feedstock-quality-and-optimization-strategies
#16
REVIEW
M Reyes-Torres, E R Oviedo-Ocaña, I Dominguez, D Komilis, A Sánchez
Green waste (GW) is an important fraction of municipal solid waste (MSW). The composting of lignocellulosic GW is challenging due to its low decomposition rate. Recently, an increasing number of studies that include strategies to optimize GW composting appeared in the literature. This literature review focuses on the physicochemical quality of GW and on the effect of strategies used to improve the process and product quality. A systematic search was carried out, using keywords, and 447 papers published between 2002 and 2018 were identified...
April 27, 2018: Waste Management
https://www.readbyqxmd.com/read/29709176/ultrasensitive-detection-of-escherichia-coli-o157-h7-by-immunomagnetic-separation-and-selective-filtration-with-nbt-bcip-signal-amplification
#17
Seong U Kim, Eun-Jung Jo, Hyoyoung Mun, Yuseon Noh, Min-Gon Kim
We here report an enhanced colorimetric method using enzymatic amplification with nitroblue tetrazolium (NBT)/5-bromo-4-chloro-3-indolyl-phosphate (BCIP) precipitation for the ultrasensitive detection of Escherichia coli O157:H7 through immunomagnetic separation (IMS)-selective filtration. We constructed a sensor by conjugating biotinylated anti-E. coli O157:H7 antibody and streptavidin-alkaline phosphatase (STA-AP) to the surface of magnetic nanoparticles (MNPs). The large E. coli O157:H7-conjugates complexes did not penetrate through the pore and remained on the membrane filter surface...
April 30, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29707794/mechanical-and-moisture-barrier-properties-of-corn-distarch-phosphate-film-influenced-by-modified-microcrystalline-corn-straw-cellulose
#18
Xinru Shao, Haitao Sun, Ruiping Jiang, Ting Qin, Zhongsu Ma
BACKGROUND: In this paper, a novel modified microcrystalline corn straw cellulose (MMCSC) was prepared by ultrasonic/microwave-assisted treatment. Effective incorporation of MMCSC into corn distarch phosphate (CDP) based composite films were investigated. RESULTS: As the proportion of MMCSC was increased, tensile strength increased initially before decreasing, and the elongation at break always decreased. The composite film of MMCSC20 showed the lowest water vapor permeability (2...
April 29, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29703271/determination-of-aflatoxins-and-ochratoxin-a-in-traditional-turkish-concentrated-fruit-juice-products-by-multi-immunoaffinity-column-cleanup-and-lc-fluorescence-detection-single-laboratory-validation
#19
Tugrul Kaymak, Levent Türker, Hüseyin Tulay, Joerg Stroka
Background : Pekmez and pestil are traditional Turkish foods made from concentrated grapejuice, which can be contaminated with mycotoxins such as aflatoxins and ochratoxin A (OTA). Objective : To carry out a single-laboratory validation of a method to simultaneously determine aflatoxins B1 , B₂, G1 , and G₂ and ochratoxin A in pekmez and pestil. Methods : The homogenized sample is extracted with methanol-water (80 + 20) using a high-speed blender. The (sample) extract is filtered, diluted with phosphate-buffered saline solution, and applied to a multi-immunoaffinity column (AFLAOCHRA PREP®)...
April 27, 2018: Journal of AOAC International
https://www.readbyqxmd.com/read/29696542/comparative-analysis-of-cadmium-induced-stress-responses-by-the-aromatic-and-non-aromatic-rice-genotypes-of-west-bengal
#20
Snehalata Majumdar, Bratati Chakraborty, Rita Kundu
Constant exposure of the living ecosystems to heavy metals, like cadmium (Cd), induces a detectable change at the biochemical and genetic level. Repeated application of phosphate fertilizers in paddy fields, leads to increase in Cd content of soil. Cd being highly mobile is transported to shoot and grain, thereby entering into the food chain of animal system. In the present study, treatment of 7-day old rice seedlings with 10 μM cadmium chloride resulted in Cd toxicity across the seven non-aromatic and six aromatic rice cultivars and landraces used for the study...
April 25, 2018: Environmental Science and Pollution Research International
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