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Phosphate food

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https://www.readbyqxmd.com/read/28330134/phytases-from-enterobacter-and-serratia-species-with-desirable-characteristics-for-food-and-feed-applications
#1
Harpreet Kaur Kalsi, Rajveer Singh, Harcharan Singh Dhaliwal, Vinod Kumar
Phytases are enzymes of great industrial importance with wide range of applications in animal and human nutrition. These catalyze the hydrolysis of phosphomonoester bonds in phytate, thereby releasing lower forms of myo-inositol phosphates and inorganic phosphate. Addition of phytase to plant-based foods can improve its nutritional value and increase mineral bioavailability by decreasing nutritional effect of phytate. In the present investigation, 43 phytase positive bacteria on PSM plates were isolated from different sources and characterized for phytase activity...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28327528/anti-obesity-effect-of-bombus-ignitus-queen-glycosaminoglycans-in-rats-on-a-high-fat-diet
#2
Mi Young Ahn, Ban Ji Kim, Ha Jeong Kim, Hyung Joo Yoon, Sang Duck Jee, Jae Sam Hwang, Kun-Koo Park
The mechanism of functional insect glycosaminoglycan (GAG) on obesity caused a high fat diet has not yet been elucidated. Therefore, insect glycosaminoglycans derived from Isaria sinclairii, Bombus ignitus (a type of bumblebee) queen, and Gryllus bimaculatus were purified and investigated as a potential functional food. 14-week old male Wistar rats were fed a high-fat diet (HFD) for 6 weeks. There were five groups that received daily intraperitoneal administration of phosphate buffered saline (PBS, control), GbG (GAG from Gryllus bimaculatus) 10 mg/kg, ISG (GAG from Isaria sinclairii) 10 mg/kg, IQG (GAG from Bombus ignites) 10 mg/kg, or Pravastatin (2 mg/kg)...
March 22, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28327345/immunoassays-for-riboflavin-and-flavin-mononucleotide-using-antibodies-specific-to-d-ribitol-and-d-ribitol-5-phosphate
#3
G Ravi, Yeldur P Venkatesh
Riboflavin (vitamin B2), a water-soluble vitamin, plays a key role in maintaining human health. Though, numerous methods have been reported for the determination of total riboflavin (TRF) content in foods and biological samples, very few methods are reported for quantifying riboflavin and its coenzymes [flavin mononucleotide (FMN); flavin adenine dinucleotide (FAD)] individually. Recently, we have demonstrated that antibodies specific to d-ribitol and d-ribitol-5-phosphate also recognize riboflavin and FMN, respectively, and not vice-versa...
March 18, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28321680/environmental-effects-of-crude-oil-spill-on-the-physicochemical-and-hydrobiological-characteristics-of-the-nun-river-niger-delta
#4
Augustine O Ifelebuegu, Justina E Ukpebor, Anita U Ahukannah, Ernest O Nnadi, Stephen C Theophilus
Oil spill pollution has remained a source of several international litigations in the Niger Delta region of Nigeria. In this paper, we examined the impacts of small recurrent crude oil spills on the physicochemical, microbial and hydrobiological properties of the Nun River, a primary source of drinking water, food and recreational activities for communities in the region. Samples were collected from six sampling points along the stretch of the lower Nun River over a 3-week period. Temperature, pH salinity, turbidity, total suspended solids, total dissolved solids, dissolved oxygen, phosphate, nitrate, heavy metals, BTEX, PAHs and microbial and plankton contents were assessed to ascertain the quality and level of deterioration of the river...
April 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28318631/the-antihelmenthic-phosphate-niclosamide-impedes-renal-fibrosis-by-inhibiting-homeodomain-interacting-protein-kinase-2-expression
#5
Xiaoyan Chang, Xin Zhen, Jixing Liu, Xiaomei Ren, Zheng Hu, Zhanmei Zhou, Fengxin Zhu, Ke Ding, Jing Nie
Renal fibrosis is the final common pathway of all varieties of progressive chronic kidney disease. However, there are no effective therapies to prevent or slow the progression of renal fibrosis. Niclosamide is a US Food and Drug Administration-approved oral antihelminthic drug used for treating most tapeworm infections. Here, we demonstrated that phosphate niclosamide, the water-soluble form of niclosamide, significantly reduced proteinuria, glomerulosclrotic lesions, and interstitial fibrosis in a murine model of adriamycin nephropathy...
March 16, 2017: Kidney International
https://www.readbyqxmd.com/read/28316482/monitoring-of-bioluminescent-lactobacillus-plantarum-in-a-complex-food-matrix
#6
Gi-Seong Moon, Arjan Narbad
A bioluminescent Lactobacillus plantarum (pLuc2) strain was constructed. The luminescent signal started to increase during the early exponential phase and reached its maximum in the mid-exponential phase in a batch culture of the strain. The signal detection sensitivity of the strain was the highest in PBS (phosphate buffered saline), followed by milk and MRS broth, indicating that the sensitivity was influenced by the matrix effect. The strain was used in millet seed fermentation which has a complex matrix and native lactic acid bacteria (LAB)...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/28303615/rare-earth-elements-and-select-actinoids-in-the-canadian-house-dust-study
#7
Pat E Rasmussen, Christine Levesque, Marc Chénier, H David Gardner
Nationally representative baseline data are presented for rare earth elements (REE), plus thorium (Th) and uranium (U) in house dust sampled from 1025 urban homes, in units of concentrations (μg g(-1) ), loadings (μg m(-2) ) and loading rates (ng m(-2) day(-1) ). Spearman rank correlations indicate that, in addition to outdoor sources, consumer products and building materials can influence indoor dust concentrations of REE, Th and U. Correlations (p <0.01) with numbers of occupants, dogs and cats suggest soil track-in...
March 17, 2017: Indoor Air
https://www.readbyqxmd.com/read/28298956/phosphorus-regulation-in-chronic-kidney-disease
#8
Wadi N Suki, Linda W Moore
Serum phosphorus levels stay relatively constant through the influence of multiple factors-such as parathyroid hormone, fibroblast growth factor 23, and vitamin D-on the kidney, bone, and digestive system. Whereas normal serum phosphorus ranges between 3 mg/dL to 4.5 mg/dL, large cross-sectional studies have shown that even people with normal kidney function are sometimes found to have levels ranging between 1.6 mg/dL and 6.2 mg/dL. While this may partially be due to diet and the factors mentioned above, total understanding of these atypical ranges of serum phosphorus remains uncertain...
October 2016: Methodist DeBakey Cardiovascular Journal
https://www.readbyqxmd.com/read/28295356/red-blood-cell-metabolic-responses-to-refrigerated-storage-rejuvenation-and-frozen-storage
#9
Angelo D'Alessandro, Alan D Gray, Zbignew M Sczcepiorkowski, Kirk Hansen, Louise H Herschel, Larry J Dumont
BACKGROUND: Storage of red blood cells (RBCs) under blood bank conditions promotes metabolic modulation within the RBC. This "metabolic storage lesion" may affect the quality and safety of the transfused RBCs. The aim of this study is to determine the metabolic changes in stored RBCs over 42 days of routine storage followed by a US Food and Drug Administration-approved method of rejuvenation, freezing, and preparation for transfusion. STUDY DESIGN AND METHODS: We exploited a mass spectrometry-based metabolomics approach to monitor 42-day-stored citrate phosphate dextrose/AS-1 RBCs (n = 29) that were rejuvenated, glycerolized and frozen, then thawed and deglycerolized, and held for 24 hours at 1 to 6ºC in saline-glucose...
March 10, 2017: Transfusion
https://www.readbyqxmd.com/read/28290293/comparative-proteomic-analysis-of-milk-derived-exosomes-in-human-and-bovine-colostrum-and-mature-milk-samples-by-itraq-coupled-lc-ms-ms
#10
Mei Yang, Dahe Song, Xueyan Cao, Rina Wu, Biao Liu, Wenhui Ye, Junrui Wu, Xiqing Yue
Exosomes are membranous vesicles found in biological fluids with important functions. However, milk-derived exosome proteins from humans and bovines have not been studied in detail. The advanced iTRAQ proteomic approach was used to analyze milk-derived exosomes in human and bovine colostrum and mature milk samples. A total of 920 milk exosome proteins were identified and quantified. Among these, 575 differentially expressed exosome proteins (P<0.05) were found. Multivariate analysis, gene ontology (GO) annotation and the KEGG pathway were used to interpret the identified proteins...
February 2017: Food Research International
https://www.readbyqxmd.com/read/28288591/correlates-of-thymus-size-and-changes-during-treatment-of-children-with-severe-acute-malnutrition-a-cohort-study
#11
Maren Johanne Heilskov Rytter, Hanifa Namusoke, Christian Ritz, Kim F Michaelsen, André Briend, Henrik Friis, Dorthe Jeppesen
BACKGROUND: The impairment of immune functions associated with malnutrition may be one reason for the high mortality in children with severe acute malnutrition (SAM), and thymus atrophy has been proposed as a marker of this immunodeficiency. The aim of this study was to identify nutritional and clinical correlates of thymus size in children with SAM, and predictors of change in thymus size with nutritional rehabilitation. METHODS: In an observational study among children aged 6-59 months admitted with SAM in Uganda, we measured thymus area by ultrasound on hospital admission to treatment with F75 and F100, on hospital discharge and after 8 weeks of nutritional rehabilitation with ready-to-use therapeutic food, as well as in well-nourished healthy children...
March 14, 2017: BMC Pediatrics
https://www.readbyqxmd.com/read/28286319/establishment-of-crispr-cas9-in-alternaria-alternata
#12
Maximilian Wenderoth, Christoph Pinecker, Benjamin Voß, Reinhard Fischer
The filamentous fungus Alternaria alternata is a potent producer of many secondary metabolites, some of which like alternariol or alternariol-methyl ether are toxic and/or cancerogenic. Many Alternaria species do not only cause post-harvest losses of food and feed, but are aggressive plant pathogens. Despite the great economic importance and the large number of research groups working with the fungus, the molecular toolbox is rather underdeveloped. Gene deletions often result in heterokaryotic strains and therefore, gene-function analyses are rather tedious...
March 10, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28285658/competition-assays-revealed-paenibacillus-pasadenensis-strain-r16-as-a-novel-antifungal-agent
#13
Alessandro Passera, Giovanni Venturini, Giovanna Battelli, Paola Casati, Francesca Penaca, Fabio Quaglino, Piero Attilio Bianco
The development of new sustainable containment strategies of plant diseases is very important to guarantee food security while reducing the environmental impact of agriculture. Research of new biocontrol agents is a long and difficult process that involves several steps that start from the identification of possible candidates which, for example, show antibiotic activities, and ends with in field, large scale trials. In this study, the plant growth promoting potential and antifungal effect exerted by a novel, putative candidate biocontrol agent, strain R16, identified as Paenibacillus pasadenensis by sequence analysis of 16S rRNA and rpoB genes, against three important plant pathogenic fungi (Botrytis cinerea, Fusarium verticillioides, and Phomopsis viticola), were assessed...
May 2017: Microbiological Research
https://www.readbyqxmd.com/read/28284640/exposure-to-organophosphorus-pesticides-in-norwegian-mothers-and-their-children-diurnal-variability-in-concentrations-of-their-biomarkers-and-associations-with-food-consumption
#14
Enrique Cequier, Amrit Kaur Sakhi, Line Småstuen Haug, Cathrine Thomsen
Several studies have suggested that exposure to organophosphorus (OP) pesticides is detrimental for health, and in particular for children where moderate doses may have a negative impact on the neurodevelopment. This study surveys levels of the 6 non-specific urinary metabolites (dialkyl phosphates (DAPs)) of OP pesticides in Norwegian mothers (n=48) and their children (n=54), and examines the diurnal variation in concentrations as well as associations with consumption of specific food products. The highest median concentration measured in urine was found for dimethyl thiophosphate (5...
March 8, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28280870/metabolic-engineering-for-high-glycerol-production-by-the-anaerobic-cultures-of-saccharomyces-cerevisiae
#15
Marta V Semkiv, Kostyantyn V Dmytruk, Charles A Abbas, Andriy A Sibirny
Glycerol is used by the cosmetic, paint, automotive, food, and pharmaceutical industries and for production of explosives. Currently, glycerol is available in commercial quantities as a by-product from biodiesel production, but the purity and the cost of its purification are prohibitive. The industrial production of glycerol by glucose aerobic fermentation using osmotolerant strains of the yeasts Candida sp. and Saccharomyces cerevisiae has been described. A major drawback of the aerobic process is the high cost of production...
March 9, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28280335/nanoparticle-abraxane-possesses-impaired-proliferation-in-a549-cells-due-to-the-underexpression-of-glucosamine-6-phosphate-n-acetyltransferase-1-gnpnat1-gna1
#16
Minzhi Zhao, Haiyun Li, Yan Ma, He Gong, Shu Yang, Qiaojun Fang, Zhiyuan Hu
Abraxane (Abr), a US Food and Drug Administration-approved albumin-bound nanoparticle applied for the treatment of non-small-cell lung cancer, has been reported to be more effective than paclitaxel (PTX). To further understand the molecular mechanisms that produce this superior drug efficacy of Abr, a quantitative proteomic approach has been applied to investigate the global protein expression profiles of lung cancer cell A549 treated with Abr and PTX. Only one protein, namely, glucosamine 6-phosphate N-acetyltransferase 1 (GNA1), showed significant differential expression (P<0...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28258188/lysine-post-translational-modification-of-glyceraldehyde-3-phosphate-dehydrogenase-regulates-hepatic-and-systemic-metabolism
#17
Simon T Bond, Kirsten F Howlett, Greg M Kowalski, Shaun Mason, Timothy Connor, Adrian Cooper, Victor Streltsov, Clinton R Bruce, Ken R Walder, Sean L McGee
Reciprocal regulation of hepatic glycolysis and gluconeogenesis contributes to systemic metabolic homeostasis. Recent evidence from lower order organisms has found that reversible post-translational modification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), particularly acetylation, contributes to the reciprocal regulation of glycolysis/gluconeogenesis. However, whether this occurs in mammalian hepatocytes in vitro or in vivo is unknown. Several proteomics studies have identified 4 lysine residues in critical regions of mammalian GAPDH that are altered by multiple post-translational modifications...
March 3, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28255652/attenuation-of-liver-cancer-development-by-oral-glycerol-supplementation-in-the-rat
#18
Alejo M Capiglioni, Florencia Lorenzetti, Ariel D Quiroga, Juan P Parody, María T Ronco, Gerardo B Pisani, María C Carrillo, María P Ceballos, María de Luján Alvarez
PURPOSE: Glycerol usage is increasing in food industry for human and animal nutrition. This study analyzed the impact of glycerol metabolism when orally supplemented during the early stage of rat liver carcinogenesis. METHODS: Wistar rats were subjected to a 2-phase model of hepatocarcinogenesis (initiated-promoted, IP group). IP animals also received glycerol by gavage (200 mg/kg body weight, IPGly group). RESULTS: Glycerol treatment reduced the volume of preneoplastic lesions by decreasing the proliferative status of liver foci, increasing the expression of p53 and p21 proteins and reducing the expression of cyclin D1 and cyclin-dependent kinase 1...
March 2, 2017: European Journal of Nutrition
https://www.readbyqxmd.com/read/28254011/event-specific-qualitative-and-quantitative-detection-of-five-genetically-modified-rice-events-using-a-single-standard-reference-molecule
#19
Jae-Hwan Kim, Saet-Byul Park, Hyo-Jeong Roh, Min-Ki Shin, Gui-Im Moon, Jin-Hwan Hong, Hae-Yeong Kim
One novel standard reference plasmid, namely pUC-RICE5, was constructed as a positive control and calibrator for event-specific qualitative and quantitative detection of genetically modified (GM) rice (Bt63, Kemingdao1, Kefeng6, Kefeng8, and LLRice62). pUC-RICE5 contained fragments of a rice-specific endogenous reference gene (sucrose phosphate synthase) as well as the five GM rice events. An existing qualitative PCR assay approach was modified using pUC-RICE5 to create a quantitative method with limits of detection correlating to approximately 1-10 copies of rice haploid genomes...
July 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28250659/antidiabetic-effects-of-aqueous-and-dichloromethane-methanol-stem-bark-extracts-of-pterocarpus-soyauxii-taub-papilionaceae-on-streptozotocin-induced-diabetic-rats
#20
Marie Claire Tchamadeu, Paul Désiré Djomeni Dzeufiet, Nelly Blaes, Jean-Pierre Girolami, Pierre Kamtchouing, Théophile Dimo
AIM OF THE STUDY: The aim is to evaluate the hypoglycemic and antidiabetic effects of aqueous and CH2Cl2/CH3OH stem bark extracts of Pterocarpus soyauxii Taub in normal and diabetic rats. MATERIALS AND METHODS: Streptozotocin (STZ)-induced diabetic and normal adult Wistar rats were orally administered with aqueous and CH2Cl2/CH3OH plant extracts of P. soyauxii at various doses (38-300 mg/kg) in a single administration. In addition, STZ-induced diabetic rats received prolonged daily administration for 14 days...
January 2017: Pharmacognosy Research
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