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https://www.readbyqxmd.com/read/28098926/relevance-of-osteoclast-specific-enzyme-activities-in-cell-based-in-vitro-resorption-assays
#1
A Bernhardt, K Koperski, M Schumacher, M Gelinsky
Cell-based in vitro resorption assays are an important tool to simulate the in vivo biodegradation of resorbable bone graft materials and to predict their clinical performance. The present study analyses the activity of osteoclast-specific enzymes as potential surrogate measures for classical pit assay, which is not applicable on irregular structured materials. Osteoclasts derived from human peripheral blood mononuclear cells were cultivated on different surfaces: calcium phosphate bone cements (CPC), dentin discs, osteoblast-derived extracellular matrix (ECM) and tissue culture polystyrene as control...
January 18, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28098745/effect-of-freeze-dried-allograft-bone-with-human-basic-fibroblast-growth-factor-containing-a-collagen-binding-domain-from-clostridium-histolyticum-collagenase-on-bone-formation-after-lumbar-posterolateral-fusion-surgery-in-rats
#2
Gen Inoue, Kentaro Uchida, Osamu Matsushita, Hisako Fujimaki, Wataru Saito, Masayuki Miyagi, Hiroyuki Sekiguchi, Nozomu Nishi, Seiji Ohtori, Mizuki Yogoro, Masashi Takaso
STUDY DESIGN: An experimental study. OBJECTIVE: To evaluate the effectiveness of freeze-dried bone allograft (FDBA) with basic fibroblast growth factor (bFGF) fused with the polycystic kidney disease domain (PKD) and the collagen-biding domain (CBD) of Clostridium histolyticum collagenase, for the acceleration of lumbar posterolateral fusion in rats. SUMMARY OF BACKGROUND DATA: Reports indicate bFGF is an effective growth factor with osteogenic potential for promoting bone regeneration, although its efficiency decreases rapidly following its diffusion in body fluid from the host site...
January 16, 2017: Spine
https://www.readbyqxmd.com/read/28098717/effectiveness-of-autologous-fat-grafting-in-adherent-scars-results-obtained-by-a-comprehensive-scar-evaluation-protocol
#3
Fabio Caviggioli, Giorgio Pajardi, Andrea Rimondo, Guido Cornegliani, Marco Klinger, Francesco Klinger
No abstract text is available yet for this article.
January 13, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28098711/primary-septal-cartilage-graft-for-the-unilateral-cleft-rhinoplasty
#4
Ting-Chen Lu, Chuan-Fong Yao, Susie Lin, Chun-Shin Chang, Philip Kuo-Ting Chen
PROPOSE: Since 2006, we have explored the option of using septal cartilage as an alar rim graft on the cleft side during primary rhinoplasty to improve nasal symmetry. The aim of this study was to compare the nasal shape with or without rim graft. METHODS: A total of 98 patients with unilateral complete cleft lip and palate were included; 39 patients had septal cartilage as the rim graft, 59 patients did not. Measurements of the nostril height, nostril width, 1/4 medial part of nostril height, nostril area, nasal dome height and nostril axis were made on the cleft and non-cleft side...
January 13, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28098576/grafting-and-dental-implantation-in-patients-with-jawbone-cavitation-case-series-and-3-year-follow-up
#5
Ya-Wei Chen, Miguel Simancas-Pallares, Mauro Marincola, Sung-Kiang Chuang
PURPOSE: Jawbone cavitation (BC) is not uncommon and is considered to be related to some cases of unexpected implant displacement into deep jawbone space. Here, a series of cases with BC is described, in which the lesions were accidentally found and successfully treated by bone grafting and dental implantation. METHODS: Thirty-four partially edentulous patients who were found to have BC during dental implant surgeries were included in this study. Alloplast bone substitute (β-tricalcium phosphate) grafting with immediate or staged locking-taper implant placement was performed...
January 16, 2017: Implant Dentistry
https://www.readbyqxmd.com/read/28098566/comprehensive-surgical-orthodontic-treatment-of-class-iii-malocclusion-in-cleft-patient-involving-minimally-invasive-surgery-case-report-and-literature-review
#6
Goran Stojkovic, Miodrag Stojkovic, Jasna Stojkovic, Dejan Nikolic, Zoran Stajcic
AIM: Surgical and orthodontic treatment of a teenage cleft patient. CASE REPORT: Authors describe the case of a 13 year old female cleft patient presented with class III malocclusion RESULT: The patient underwent comprehensive surgical secondary bone grafting and orthodontic treatment. Stable skeletal and occlusal class I relationship was achived and maintained in the post treatment observation period till the age of 16. DISCUSSION: Although several authors suggests primary gingivoperiosteoplasty, other advocates that such early intervention can cause later restrictions in maxillary growth...
December 19, 2016: Annali Italiani di Chirurgia
https://www.readbyqxmd.com/read/28098565/bio-engineering-tissue-and-v-a-c-therapy-a-new-method-for-the-treatment-of-extensive-necrotizing-infection-in-the-diabetic-foot
#7
Andrea Armenio, Daniela Anna Cutrignelli, Maria Luisa Nardulli, Giulio Maggio, Giuseppe Memeo, Valerio De Santis, Giuseppe Giudice, Cosmo Maurizio Ressa
AIM: The aim of the study is to compare the standard care for progressive necrotizing infection in diabetic foot with a treatment protocol based on the association between autologous fibroblast grafts and vacuum-assisted closure therapy (V.A.C.). MATERIAL OF STUDY: A retrospective matched Case-Control study was carried out on 20 patients with diabetic foot infection, 10 treated with the standard care and 10 with our new protocol. Inclusion criteria were: acute diabetic foot necrosis (Wagner III and IV), ulcer size (30 to 80 cm2), tendon and bone exposure...
December 6, 2016: Annali Italiani di Chirurgia
https://www.readbyqxmd.com/read/28098444/a-heterogeneous-electrocatalyst-with-molecular-cobalt-ions-serving-as-the-center-of-active-sites
#8
Jiong Wang, Xiaoming Ge, Zhaolin Liu, Larissa Thia, Ya Yan, Wei Xiao, Xin Wang
Molecular Co2+ ions were grafted onto doped graphene in a coordination environment, resulting in the formation of molecularly well-defined, highly active electrocatalytic sites at a heterogeneous interface for the oxygen evolution reaction (OER). The S dopants of graphene are suggested to be one of the binding sites and to be responsible for improving the intrinsic activity of the Co sites. The turnover frequency of such Co sites is greater than that of many Co-based nanostructures and IrO2 catalysts. Through a series of carefully designed experiments, the pathway for the evolution of the Co cation-based molecular catalyst for the OER was further demonstrated on such a single Co-ion site for the first time...
January 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28098414/integrated-photo-grafted-molecularly-imprinted-polymers-with-a-cellulose-acetate-membrane-for-the-extraction-of-melamine-from-dry-milk-before-hplc-analysis
#9
Behrouz Akbari-Adergani, Gholam-Hossein Sadeghian, Alireza Alimohammadi, Zahra Esfandiari
In this study, a new separation technique based on membrane extraction is described for the determination of melamine in dry milk. The water-compatible cellulose acetate membrane, which is photo-grafted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen and melamine as the template molecule. The characterization of the polymeric membrane was performed by Fourier transmission infrared spectroscopy and scanning electron microscopy...
January 18, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28098167/electrocatalytic-activity-of-lithium-polysulfides-adsorbed-into-porous-tio2-coated-mwcnts-hybrid-structure-for-lithium-sulfur-batteries
#10
Xiulin He, Huijie Hou, Xiqing Yuan, Long Huang, Jingping Hu, Bingchuan Liu, Jingyi Xu, Jia Xie, Jiakuan Yang, Sha Liang, Xu Wu
Lithium-sulfur batteries have attracted great attention because of their high energy density, environmental friendliness, natural abundance and intrinsically low cost of sulfur. However, their commercial applications are greatly hindered by rapid capacity decay due to poor conductivity of electrode, fast dissolution of the intermediate polysulfides into the electrolyte, and the volume expansion of sulfur. Herein, we report a novel composite MWCNTs@TiO2-S nanostructure by grafting TiO2 onto the surface of MWCNTs, followed by incorporating sulfur into the composite...
January 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28097889/titanium-cages-versus-autogenous-iliac-crest-bone-grafts-in-anterior-cervical-discectomy-and-fusion-treatment-of-patients-with-cervical-degenerative-diseases-a-systematic-review-and-meta-analysis
#11
Ming-Hao Shao, Fan Zhang, Jun Yin, Hao-Cheng Xu, Fei-Zhou Lyu
OBJECTIVE: A systematic review and partial meta-analysis is conducted to compare the efficacy and safety of anterior cervical decompression and fusion procedures employing either rectangular titanium cages or iliac crest autografts in patients suffering from cervical degenerative disc diseases. METHODS: Medline, PubMed, CENTRAL, and Google Scholar databases were searched until June 2015, using keywords cervical discectomy; bone transplantation; titanium cages; iliac crest autografts...
January 18, 2017: Current Medical Research and Opinion
https://www.readbyqxmd.com/read/28097811/fc-silent-anti-cd154-domain-antibody-effectively-prevents-non-human-primate-renal-allograft-rejection
#12
Steven C Kim, Walter Wakwe, Laura B Higginbotham, David V Mathews, Cynthia P Breeden, Allison C Stephenson, Joe Jenkins, Elizabeth Strobert, Karen Price, Laura Price, Robert Kuhn, Haiqing Wang, Aaron Yamniuk, Suzanne Suchard, Alton B Farris, Thomas C Pearson, Christian P Larsen, Mandy L Ford, Anish Suri, Steven Nadler, Andrew B Adams
The advent of costimulation blockade provides the prospect for targeted therapy with improved graft survival in transplant patients. Perhaps the most effective costimulation blockade in experimental models is the use of reagents to block the CD40/CD154 pathway. Unfortunately, successful clinical translation of anti-CD154 therapy has not been achieved. In an attempt to develop an agent that is as effective as previous CD154 blocking antibodies but lacks the risk of thromboembolism, we evaluated the efficacy and safety of a novel anti-human CD154 domain antibody (dAb, BMS-986004)...
January 17, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28097805/complement-polymorphisms-in-kidney-transplantation-critical-in-graft-rejection
#13
L A Michielsen, A D van Zuilen, I S Muskens, M C Verhaar, H G Otten
The complement system, as part of the innate immune system, plays an important role in renal transplantation. Complement is involved in the protection against foreign organisms and clearance of apoptotic cells but can also cause injury to the renal allograft for instance via antibody binding or in ischemia reperfusion injury. Numerous polymorphisms in complement factors have been identified thus far; some of them result in different functionalities or alter complement levels. In this review, we provide an overview of the literature on the role of complement polymorphisms in renal transplantation...
January 17, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28097804/adult-living-donor-liver-transplantation-for-acute-on-chronic-liver-failure-in-high-model-for-end-stage-liver-disease-score-patients
#14
D-B Moon, S-G Lee, W-H Kang, G-W Song, D-H Jung, G-C Park, H-D Cho, E-K Jwa, W-J Kim, T-Y Ha, H-J Kim
The large volume of adult living donor liver transplantations (ALDLTs) at our center affords a unique opportunity to examine the impact of acute-on-chronic liver failure (ACLF) among high-MELD score patients. From February 1998 to March 2010, 1958 cirrhotic recipients were analyzed to study the relationship between MELD scores and ALDLT outcomes. A total of 327 high-MELD score recipients were categorized into ACLF and non-ACLF groups, and their outcomes were compared. The five-year graft and patient survival in the high MELD group were 75...
January 17, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28097749/positive-contrast-from-cells-labeled-with-iron-oxide-nanoparticles-quantitation-of-imaging-data
#15
Sergey Magnitsky, Jinjin Zhang, Djaudat Idiyatullin, Geetha Mohan, Michael Garwood, Nancy E Lane, Sharmila Majumdar
PURPOSE: Conventional T2 -weighted MRI produces a hypointense signal from iron-labeled cells, which renders quantification unfeasible. We tested a SWeep Imaging with Fourier Transformation (SWIFT) MRI pulse sequence to generate a quantifiable hyperintense signal from iron-labeled cells. METHODS: Mesenchymal stem cells (MSCs) were labeled with different concentrations of iron oxide particles and examined for cell viability, proliferation, and differentiation. The SWIFT sequence was optimized to detect and quantify the amount of iron in the muscle tissue after injection of iron oxide solution and iron-labeled MSCs...
January 17, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28097682/evaluation-of-perforated-demineralized-dentin-scaffold-on-bone-regeneration-in-critical-size-sheep-iliac-defects
#16
Md Arafat Kabir, Masaru Murata, Toshiyuki Akazawa, Kaoru Kusano, Katsuhisa Yamada, Manabu Ito
OBJECTIVES: Regenerating critical-size bone injury is a major problem that continues to inspire the design of new graft materials. Therefore, tissue engineering has become a novel approach for targeting bone regeneration applications. Human teeth are a rich source of stem cells, matrix, trace metal ions, and growth factors. A vital tooth-derived demineralized dentin matrix is acid-insoluble and composed of cross-linked collagen with growth factors. In this study, we recycled human non-functional tooth into a unique geometric dentin scaffold, entitled perforated root-demineralized dentin matrix (PR-DDM)...
January 17, 2017: Clinical Oral Implants Research
https://www.readbyqxmd.com/read/28097534/successful-treatment-with-low-dose-nivolumab-in-refractory-hodgkin-lymphoma-after-allogeneic-stem-cell-transplantation
#17
Makoto Onizuka, Minoru Kojima, Keiko Matsui, Shinichiro Machida, Masako Toyosaki, Yasuyuki Aoyama, Hidetsugu Kawai, Jun Amaki, Ryujiro Hara, Akifumi Ichiki, Yoshiaki Ogawa, Hiroshi Kawada, Naoya Nakamura, Kiyoshi Ando
Previous studies have reported that an antibody that blocks programmed cell death 1 (PD-1) has therapeutic activity in patients with refractory/relapsed Hodgkin lymphoma (HL). However, the safety and efficacy of these agents in the post-allogeneic stem cell transplantation (allo-SCT) setting are not well known. Here, we describe a patient who was diagnosed as classical HL and treated with five regimens of chemotherapies with autologous SCT. Complete remission (CR) was not achieved following this initial treatment, so we performed allo-SCT from an HLA-matched sibling donor...
January 17, 2017: International Journal of Hematology
https://www.readbyqxmd.com/read/28097523/intravascular-ultrasound-characterization-of-a-tissue-engineered-vascular-graft-in-an-ovine-model
#18
Victoria K Pepper, Elizabeth S Clark, Cameron A Best, Ekene A Onwuka, Tadahisa Sugiura, Eric D Heuer, Lilamarie E Moko, Shinka Miyamoto, Hideki Miyachi, Darren P Berman, Sharon L Cheatham, Joanne L Chisolm, Toshiharu Shinoka, Christopher K Breuer, John P Cheatham
Patients who undergo implantation of a tissue-engineered vascular graft (TEVG) for congenital cardiac anomalies are monitored with echocardiography, followed by magnetic resonance imaging or angiography when indicated. While these methods provide data regarding the lumen, minimal information regarding neotissue formation is obtained. Intravascular ultrasound (IVUS) has previously been used in a variety of conditions to evaluate the vessel wall. The purpose of this study was to evaluate the utility of IVUS for evaluation of TEVGs in our ovine model...
January 17, 2017: Journal of Cardiovascular Translational Research
https://www.readbyqxmd.com/read/28097486/the-therapeutic-effectiveness-of-delayed-fetal-spinal-cord-tissue-transplantation-on-respiratory-function-following-mid-cervical-spinal-cord-injury
#19
Chia-Ching Lin, Sih-Rong Lai, Yu-Han Shao, Chun-Lin Chen, Kun-Ze Lee
Respiratory impairment due to damage of the spinal respiratory motoneurons and interruption of the descending drives from brainstem premotor neurons to spinal respiratory motoneurons is the leading cause of morbidity and mortality following cervical spinal cord injury. The present study was designed to evaluate the therapeutic effectiveness of delayed transplantation of fetal spinal cord (FSC) tissue on respiratory function in rats with mid-cervical spinal cord injury. Embryonic day-14 rat FSC tissue was transplanted into a C4 spinal cord hemilesion cavity in adult male rats at 1 week postinjury...
January 17, 2017: Neurotherapeutics: the Journal of the American Society for Experimental NeuroTherapeutics
https://www.readbyqxmd.com/read/28097199/long-term-survival-after-composite-mechanical-aortic-root-replacement
#20
Khaled E Al-Ebrahim, Husain H Jabbad, Ahmad H Alqari
This report describes the long-term follow-up of the repair of a giant ascending aneurysm using a composite graft with a mechanical valve.
August 2016: Aorta (Stamford, Conn.)
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