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Molecular diagnostic methods

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https://www.readbyqxmd.com/read/28642141/added-diagnostic-value-and-impact-on-antimicrobial-therapy-of-16s-rrna-pcr-and-amplicon-sequencing-on-resected-heart-valves-in-infective-endocarditis-a-prospective-cohort-study
#1
Bart Peeters, Paul Herijgers, Kurt Beuselinck, Jan Verhaegen, Willy E Peetermans, Marie-Christine Herregods, Stefanie Desmet, Katrien Lagrou
OBJECTIVES: For adequate management and therapy of infective endocarditis (IE), identification of the causative pathogen is crucial but currently molecular testing results are not included in diagnostic criteria. The added diagnostic value and impact on antimicrobial therapy of 16S rRNA PCR and Amplicon Sequencing (16S rRNA PCR) performed on excised heart valves from IE patients was evaluated alongside with the effect of pre-operative antibiotics on the performance of blood culture (BC), valve culture (VC) and 16S rRNA PCR...
June 19, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28641501/hematological-characterizations-and-molecular-diagnostic-aspects-of-hb-wiangpapao-%C3%AE-44-ce2-pro%C3%A2-ser-%C3%AE-1-ccg-tcg-hba1-c-133c-t-a-new-%C3%AE-globin-variant-found-in-a-pregnant-thai-woman
#2
Sitthichai Panyasai, Sakorn Pornprasert
We report the hematological parameters and provide a rapid molecular analysis method for detection of Hb Wiangpapao [α44(CE2)Pro→Ser, CCG>TCG; HBA1: c.133C>T], a new α-globin variant found in a pregnant Thai woman. Her red cell indices were measured by an automated blood counter. The results were: red blood cell (RBC) count 4.03 × 10(12)/L, Hb 13.1 (g/dL), packed cell volume (PCV) 0.39 L/L, mean corpuscular volume (MCV) 97.0 fL, mean corpuscular hemoglobin (Hb) (MCH) 32.5 pg, mean corpuscular Hb concentration (MCHC) 33...
June 23, 2017: Hemoglobin
https://www.readbyqxmd.com/read/28640824/molecular-diagnosis-of-plasmodium-ovale-by-photo-induced-electron-transfer-fluorogenic-primers-pet-pcr
#3
David Akerele, Dragan Ljolje, Eldin Talundzic, Venkatachalam Udhayakumar, Naomi W Lucchi
Accurate diagnosis of malaria infections continues to be challenging and elusive, especially in the detection of submicroscopic infections. Developing new malaria diagnostic tools that are sensitive enough to detect low-level infections, user friendly, cost effective and capable of performing large scale diagnosis, remains critical. We have designed novel self-quenching photo-induced electron transfer (PET) fluorogenic primers for the detection of P. ovale by real-time PCR. In our study, a total of 173 clinical samples, consisting of different malaria species, were utilized to test this novel PET-PCR primer...
2017: PloS One
https://www.readbyqxmd.com/read/28640577/laser-ablation-molecular-isotopic-spectrometry-for-molecules-formation-chemistry-in-fs-laser-ablated-plasmas
#4
Huaming Hou, Xianglei Mao, Vassilia Zorba, Richard E Russo
Recently, laser ablated molecular isotopic spectrometry (LAMIS) has expanded its capability to explore molecules formation mechanism in laser induced plasma in addition to isotope analysis. LAMIS is powerful tool for tracking the origination of atoms that involved in formation of investigated molecules by labeling atoms with its isotopic substitution. The evolutionary formation pathways of organic molecules, especially of C2 dimers and CN radicals, were frequently reported. However, very little is known about the formation pathways for metallic radicals and heterodimers in laser ablated plasma...
June 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28639650/mechanisms-of-h-and-co-loss-from-the-uracil-nucleobase-following-low-energy-electron-irradiation
#5
Mark A Fennimore, Tolga N V Karsili, Spiridoula Matsika
Ionising radiation in cells generates secondary low energy electrons (LEEs) which can induce biomolecular damage when incident upon a particular biomolecule. Notable biomolecules include those contained within double-stranded DNA and RNA helices, which upon exposure to LEEs, may form reactive intermediate products that show detriment to their specific structures and functions. Such damaging processes are understood to proceed via dissociative electron attachment (DEA). Using anion resonance stabilisation methods, coupled to state-of-the-art electronic structure methods, and reaction path mapping, the work presented herein highlights the detailed mechanisms associated with molecular elimination of CO and H from the uracil nucleobase following LEE irradiation - providing a rationale for earlier experiments...
June 22, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28636673/evaluation-of-molecular-diagnostic-approaches-for-the-detection-of-braf-p-v600e-mutations-in-papillary-thyroid-cancer-clinical-implications
#6
Artur Kowalik, Aldona Kowalska, Agnieszka Walczyk, Renata Chodurska, Janusz Kopczyński, Magdalena Chrapek, Elżbieta Wypiórkiewicz, Małgorzata Chłopek, Liliana Pięciak, Danuta Gąsior-Perczak, Iwona Pałyga, Krzysztof Gruszczyński, Ewelina Nowak, Stanisław Góźdź
Differentiated papillary thyroid cancer (PTC) is the most common cancer of the endocrine system. PTC has a very good prognosis and a high 5 year survival rate; however, some patients are unresponsive to treatment, and their diagnosis eventually results in death. Recent efforts have focused on searching for prognostic and predictive factors that may enable treatment personalization and monitoring across the course of the disease. The presence of the BRAF mutation is considered to contribute to the risk of poor clinical course, according to American Thyroid Association (ATA) recommendations...
2017: PloS One
https://www.readbyqxmd.com/read/28634288/qsva-framework-for-rna-quality-correction-in-differential-expression-analysis
#7
Andrew E Jaffe, Ran Tao, Alexis L Norris, Marc Kealhofer, Abhinav Nellore, Joo Heon Shin, Dewey Kim, Yankai Jia, Thomas M Hyde, Joel E Kleinman, Richard E Straub, Jeffrey T Leek, Daniel R Weinberger
RNA sequencing (RNA-seq) is a powerful approach for measuring gene expression levels in cells and tissues, but it relies on high-quality RNA. We demonstrate here that statistical adjustment using existing quality measures largely fails to remove the effects of RNA degradation when RNA quality associates with the outcome of interest. Using RNA-seq data from molecular degradation experiments of human primary tissues, we introduce a method-quality surrogate variable analysis (qSVA)-as a framework for estimating and removing the confounding effect of RNA quality in differential expression analysis...
June 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28632297/n%C3%A3-lbiopsi-%C3%A3-r-inte-b%C3%A3-sta-metod-f%C3%A3-r-att-diagnostisera-lymfom-den-%C3%A3-kade-anv%C3%A3-ndningen-kan-ge-allvarliga-konsekvenser-f%C3%A3-r-diagnostik-forskning-och-behandlingsutveckling
#8
Rose-Marie Amini, Christer Sundström
Core needle biopsies for lymphoma diagnosis seriously affect diagnostics, treatment development and research Core needle biopsies (CNBs) are widely used in clinical diagnostic labs to aid in the diagnosis of malignant lymphomas and in latter years their use is increasing. CNBs provide a rapid method for obtaining tumour material and may be beneficial when the affected lymph nodes are located deep in the abdominal cavity or mediastinum and surgical excisional biopsies may be difficult to perform. However, according to the Swedish Haematopathology Quality and Standardization Committee, CNBs are insufficient for lymphoma diagnostic purposes and the guidelines state that material from surgical excisional biopsies are mandatory in order to obtain a robust histopathological evaluation of the lymph node architecture, cellular composition and growth pattern...
June 16, 2017: Läkartidningen
https://www.readbyqxmd.com/read/28631643/multidrug-resistant-tuberculosis-detection-and-characterization-of-mutations-in-mycobacterium-tuberculosis-by-genotype-mtbdrplus
#9
Rajneesh Tripathi, Shampa Anupurba
Detection of drug resistance in Mycobacterium tuberculosis by conventional phenotypic drug susceptibility testing methods requires several weeks. Therefore, molecular diagnostic tests for rapid detection of multidrug resistance tuberculosis (MDR-TB) are urgently needed. Early diagnosis helps in initiating optimal treatment which would not only enable cure of an individual patient but also will curb the transmission of drug resistance in the community. Line probe assay (LPA) has shown great promises in the diagnosis of MDR-TB...
April 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28631631/ki-67-evaluation-in-breast-cancer-the-daily-diagnostic-practice
#10
Lukasz Fulawka, Agnieszka Halon
CONTEXT: Breast cancer is the most common malignancy in females. It is routinely classified according to the WHO histological typing. However, there is also a molecular classification of breast cancer which is routinely substituted with surrogate subtypes based on expression of estrogen, progesterone, and human epidermal growth factor receptor 2 receptors and proliferation index (PI). PI is defined as the percentage of Ki-67-positive cells among overall cell population. The method commonly applied by pathologists to determine PI is visual scoring of the sample...
April 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28628614/lamphimerus-a-novel-lamp-assay-for-detecting-amphimerus-sp-dna-in-human-stool-samples
#11
William Cevallos, Pedro Fernández-Soto, Manuel Calvopiña, Cristina Fontecha-Cuenca, Hiromu Sugiyama, Megumi Sato, Julio López Abán, Belén Vicente, Antonio Muro
BACKGROUND: Amphimeriasis is a fish-borne disease caused by the liver fluke Amphimerus spp. that has recently been reported as endemic in the tropical Pacific side of Ecuador with a high prevalence in humans and domestic animals. The diagnosis is based on the stool examination to identify parasite eggs, but it lacks sensitivity. Additionally, the morphology of the eggs may be confounded with other liver and intestinal flukes. No immunological or molecular methods have been developed to date...
June 19, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28627855/multiplex-pcr-gastro-intestinal-panels-for-the-management-of-traveller-s-diarrhea-are-they-accurate-and-useful
#12
Laurence Rochat, Antony Croxatto, Serge De Vallière, Valérie D'Acremont, Blaise Genton
Until recently, the search for enteropathogens causing travellers' diarrhea was based on stool culture (Campylobacter spp., Salmonella spp. and Shigella spp.), direct microscopy with (Cryptosporidium spp.) or without specific staining (Giardia lamblia, Entamoeba histolytica) or specific antigen detection (Giardia lamblia, Entamoeba histolytica). Molecular analyses are progressively replacing traditional diagnostic methods but their clinical usefulness remains to be better defined. This article attempts to describe the advantages and disadvantages of these new molecular methods and to illustrate situations where they could be useful using clinical cases frequently encountered in the practice of travel medicine...
May 3, 2017: Revue Médicale Suisse
https://www.readbyqxmd.com/read/28627685/metabolomic-analysis-based-on-1h-nuclear-magnetic-resonance-spectroscopy-metabolic-profiles-in-tuberculous-malignant-and-transudative-pleural-effusion
#13
Cheng Wang, Jingjin Peng, Yanling Kuang, Jiaqiang Zhang, Luming Dai
Pleural effusion is a common clinical manifestation with various causes. Current diagnostic and therapeutic methods have exhibited numerous limitations. By involving the analysis of dynamic changes in low molecular weight catabolites, metabolomics has been widely applied in various types of disease and have provided platforms to distinguish many novel biomarkers. However, to the best of our knowledge, there are few studies regarding the metabolic profiling for pleural effusion. In the current study, 58 pleural effusion samples were collected, among which 20 were malignant pleural effusions, 20 were tuberculous pleural effusions and 18 were transudative pleural effusions...
June 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28626632/synchronous-mucinous-and-non-mucinous-lung-adenocarcinomas-with-different-epidermal-growth-mutational-status
#14
Rita Linhas, David Tente, Margarida Dias, Ana Barroso
In recent years, the spread of more-sensitive diagnostic methods has resulted in an increase of synchronous multiple primary lung cancer diagnosis. Nevertheless, its occurrence is still rare. Distinction between synchronous lesions from second independent primary tumors is a problem when dealing with multiple lung tumors, particularly if the histological type is the same. We present a case report of a 78-year-old female patient referred to our institution due to pneumonia. A subsequent thoracic computed tomography (CT) was performed showing two suspicious lesions, one in the right upper lobe and the other in the right inferior lobe...
2017: Respiratory Medicine Case Reports
https://www.readbyqxmd.com/read/28626584/enteric-multiplex-pcr-panels-a-new-diagnostic-tool-for-amoebic-liver-abscess
#15
T Weitzel, J Cabrera, R Rosas, L M Noriega, G Schiappacasse, V Vollrath, L Porte
Invasive amoebiasis is a life-threatening infection requiring immediate detection and treatment. However, diagnosis is challenging because conventional methods such as light microscopy and serology are unreliable. Molecular techniques are therefore considered the new diagnostic reference standard, but most of the developed assays are research tools and not widely available. Recently commercial multiplex PCR panels have been introduced which permit the simultaneous detection of multiple enteric pathogens including Entamoeba histolytica in stool samples...
July 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/28624396/hybrid-magnetic-resonance-imaging-and-positron-emission-tomography-with-fluorodeoxyglucose-to-diagnose-active-cardiac-sarcoidosis
#16
Marc R Dweck, Ronan Abgral, Maria Giovanna Trivieri, Philip M Robson, Nicolas Karakatsanis, Venkatesh Mani, Anna Palmisano, Marc A Miller, Anuradha Lala, Helena L Chang, Javier Sanz, Johanna Contreras, Jagat Narula, Valentin Fuster, Maria Padilla, Zahi A Fayad, Jason C Kovacic
OBJECTIVES: The purpose of this study was to explore the diagnostic usefulness of hybrid cardiac magnetic resonance (CMR) and positron emission tomography (PET) using 18F-fluorodeoxyglucose (FDG) for active cardiac sarcoidosis. BACKGROUND: Active cardiac sarcoidosis (aCS) is underdiagnosed and has a high mortality. METHODS: Patients with clinical suspicion of aCS underwent hybrid CMR/PET with late gadolinium enhancement (LGE) and FDG to assess the pattern of injury and disease activity, respectively...
June 9, 2017: JACC. Cardiovascular Imaging
https://www.readbyqxmd.com/read/28623535/nanotechnologies-for-in-vitro-ige-testing
#17
REVIEW
Iwan Märki, Fabien Rebeaud
PURPOSE OF REVIEW: This review discusses the recent advances in the development of IgE antibody assays based on nanotechnologies. IgE blood testing is an important part of the diagnostic workup of IgE-mediated hypersentivity. We also address the challenges in moving from an academic proof-of-concept to a product routinely used by allergy experts. RECENT FINDINGS: Several nanotechnologies have been applied to the field of IgE testing: nanoparticles are used either as a support to capture analytes or as a detection tool to enhance the measurement signal...
July 2017: Current Allergy and Asthma Reports
https://www.readbyqxmd.com/read/28621573/update-on-the-diagnostic-value-and-safety-of-stereotactic-biopsy-for-pediatric-brainstem-tumors-a-systematic-review-and-meta-analysis-of-735-cases
#18
Christina Hamisch, Philipp Kickingereder, Matthias Fischer, Thorsten Simon, Maximilian I Ruge
OBJECTIVE Recent studies have shed light on the molecular makeup of diffuse intrinsic pontine gliomas and led to the identification of potential treatment targets for these lesions, which account for the majority of pediatric brainstem tumors (pedBSTs). Therefore, stereotactic biopsy-driven molecular characterization of pedBSTs may become an important prerequisite for the management of these fatal brain tumors. The authors conducted a systemic review and meta-analysis to precisely determine the safety and diagnostic success of stereotactic biopsy of pedBSTs...
June 16, 2017: Journal of Neurosurgery. Pediatrics
https://www.readbyqxmd.com/read/28620811/serological-molecular-and-clinical-correlates-of-dengue-from-a-tertiary-care-centre-in-chennai-india
#19
Vigna Seshan, Gopalsamy Sarangan, Khaleefathullah Sheriff, Kaveri Krishnasamy, Gunasekaran Palani, Padma Srikanth
Dengue disease is caused by dengue viruses 1-4 and has been ranked by the World Health Organisation (WHO) as the fastest spreading vector-borne viral disease. Dengue is often underreported and misdiagnosed due to a wide spectrum of clinical manifestations. Diagnosis of dengue is based on clinical case definitions and laboratory methods. Newer case definitions of dengue have been formulated by clinical studies in order to improve case detection. Owing to its epidemic potential, mortality and morbidity, there is a need for a rapid and accurate diagnostic assay for dengue in order to help the clinician in the early detection of cases and to prevent disease progression...
June 16, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28620385/the-antibody-secreting-cell-response-to-infection-kinetics-and-clinical-applications
#20
REVIEW
Michael J Carter, Ruth M Mitchell, Patrick M Meyer Sauteur, Dominic F Kelly, Johannes Trück
Despite the availability of advances in molecular diagnostic testing for infectious disease, there is still a need for tools that advance clinical care and public health. Current methods focus on pathogen detection with unprecedented precision, but often lack specificity. In contrast, the host immune response is highly specific for the infecting pathogen. Serological studies are rarely helpful in clinical settings, as they require acute and convalescent antibody testing. However, the B cell response is much more rapid and short-lived, making it an optimal target for determining disease aetiology in patients with infections...
2017: Frontiers in Immunology
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