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Xiao-Bin Fang, Ying-Qi Xu, Hon-Fai Chan, Chun-Ming Wang, Qing Zheng, Fei Xiao, Mei-Wan Chen
Hepatocellular carcinoma (HCC) is an aggressive malignancy and the second leading cause of cancer death worldwide. Most current therapeutic agents lack the tumor-targeting efficiency and result in a nonselective biodistribution in the body. In our previous study, we identified a peptide Ala-Pro-Asp-Thr-Lys-Thr-Gln (APDTKTQ) that can selectively bind to the receptor of advanced glycation end-products (RAGE), an immunoglobulin superfamily cell surface molecule overexpressed during HCC malignant progression. Here, we report the design of a mixed micelles system modified with this peptide to target HCC cells...
October 21, 2016: Molecular Pharmaceutics
Qian Chen, Ligeng Xu, Chao Liang, Chao Wang, Rui Peng, Zhuang Liu
A therapeutic strategy that can eliminate primary tumours, inhibit metastases, and prevent tumour relapses is developed herein by combining adjuvant nanoparticle-based photothermal therapy with checkpoint-blockade immunotherapy. Indocyanine green (ICG), a photothermal agent, and imiquimod (R837), a Toll-like-receptor-7 agonist, are co-encapsulated by poly(lactic-co-glycolic) acid (PLGA). The formed PLGA-ICG-R837 nanoparticles composed purely by three clinically approved components can be used for near-infrared laser-triggered photothermal ablation of primary tumours, generating tumour-associated antigens, which in the presence of R837-containing nanoparticles as the adjuvant can show vaccine-like functions...
October 21, 2016: Nature Communications
Daniel J Sobczynski, Omolola Eniola-Adefeso
Plasma proteins rapidly coat the surfaces of particulate drug carriers to form a protein corona upon their injection into the bloodstream. The high presence of immunoglobulins in the corona formed on poly(lactic-co-glycolic acid) (PLGA) vascular-targeted carrier (VTC) surfaces was recently shown to negatively impact their adhesion to activated endothelial cells (aECs) in vitro. Here, we characterized the influence of anticoagulants, or their absence, on the binding efficiency of VTCs of various materials via modulation of their protein corona...
October 17, 2016: Acta Biomaterialia
Stefan W Metz, Shaomin Tian, Gabriel Hoekstra, Xianwen Yi, Michelle Stone, Katie Horvath, Michael J Miley, Joseph DeSimone, Chris J Luft, Aravinda M de Silva
Dengue virus (DENV) is the causative agent of dengue fever and dengue hemorrhagic fever. The virus is endemic in over 120 countries, causing over 350 million infections per year. Dengue vaccine development is challenging because of the need to induce simultaneous protection against four antigenically distinct DENV serotypes and evidence that, under some conditions, vaccination can enhance disease due to specific immunity to the virus. While several live-attenuated tetravalent dengue virus vaccines display partial efficacy, it has been challenging to induce balanced protective immunity to all 4 serotypes...
October 2016: PLoS Neglected Tropical Diseases
Vilwanathan Ravikumar, Renu Sankar
Nanotechnology is a comparatively new branch of science that includes harnessing the unique properties of particles that are nanometers in scale. Nanoparticles can be tailored in a precise fashion where their size, composition, and surface chemistry can be carefully controlled. The nanoprecipitation is a simple, powerful, and low-energy requiring technique, commonly used for the preparation of defined nanoparticles. Histone deacetylase inhibitor Vorinostat-loaded Poly D, L-lactide-co-glycolide (PLGA) polymeric nanoparticles were prepared by the nanoprecipitation technique...
2017: Methods in Molecular Biology
Nadeemul Hoda, Aamir Malick Saifi, Girish B Giraddi
BACKGROUND: A common sequel of tooth extraction is alveolar bone resorption. It makes the placement of dental implants difficult and creates an esthetic problem for the fabrication of conventional prostheses. Therefore, alveolar bone following tooth extraction should be preserved. AIMS AND OBJECTIVES: The present prospective study was conducted to evaluate the efficacy of the resorbable bioscaffold poly lactic co-glycolic acid (PLGA) in maintaining the alveolar height in post-extraction socket...
September 2016: Journal of Oral Biology and Craniofacial Research
Farideh Mohammadian, Alireza Abhari, Hassan Dariushnejad, Alireza Nikanfar, Younes Pilehvar-Soltanahmadi, Nosratollah Zarghami
BACKGROUND: Recently, Chrysin, as a flavone, has revealed cancer chemo-preventive activity. The present experiment utilized the PLGA-PEG-chrysin complex, and free chrysin, to evaluation of the expression of miR-22, miR-34a and miR-126 in human gastric cell line. OBJECTIVES: The purpose of this study was to examine whether nano encapsulating chrysin improves the anti-cancer effect of free chrysin on AGS human gastric cell line. METHODS: Properties of the chrysin encapsulated in PLGA-PEG nanoparticles were investigated by SEM, H NMR, and FTIR...
August 2016: Iranian Journal of Cancer Prevention
Lauren Sfakis, Tim Kamaldinov, Melinda Larsen, James Castracane, Alexander Khmaladze
Quantifying confocal images to enable location of specific proteins of interest in 3D is important for many tissue engineering applications. Quantification of protein localization is essential for evaluation of specific scaffold constructs for cell growth and differentiation for application in tissue engineering and tissue regeneration strategies. Although obtaining information regarding protein expression levels is important, the location of proteins within cells grown on scaffolds is often key to evaluating scaffold efficacy...
October 19, 2016: Tissue Engineering. Part C, Methods
Shih-Feng Chou, Kim A Woodrow
Electrospun nanofibers have the potential to achieve high drug loading and the ability to sustain drug release. Mechanical properties of the drug-incorporated fibers suggest the importance of drug-polymer interactions. In this study, we investigated the mechanical properties of electrospun polycaprolactone (PCL) and poly (D,L-lactic-co-glycolic) acid (PLGA) fibers at various blend ratios in the presence and absence of a small molecule hydrophilic drug, tenofovir (TFV). Young׳s modulus of the blend fibers showed dependence on PLGA content and the addition of the drug...
September 9, 2016: Journal of the Mechanical Behavior of Biomedical Materials
Brenda Gomes Fanchiotti, Marcella Piffer Zamprogno Machado, Letícia Camilato de Paula, Mahmut Durmuş, Tebello Nyokong, Arlan da Silva Gonçalves, André Romero da Silva
The photobleaching of an unsubstituted phthalocyanine (gallium(III) phthalocyanine chloride (GaPc)) and a substituted phthalocyanine (1,4-(tetrakis[4-(benzyloxy)phenoxy]phthalocyaninato) indium(III) chloride (InTBPPc)) was monitored for the free photosensitizers and for the phthalocyanines encapsulated into nanoparticles of PEGylated poly(D,L-lactide-co-glycolide) (PLGA-PEG). Phosphate-buffered solutions (PBS) and organic solutions of the free GaPc or the free InTBPPc, and suspensions of each encapsulated photosensitizer (2-15μmol/L) were irradiated using a laser diode of 665nm with a power of 1-104mW and a light dose of 7...
October 11, 2016: Journal of Photochemistry and Photobiology. B, Biology
Soumitra Shome, Anupam Das Talukdar, Manabendra Dutta Choudhury, Mrinal Kanti Bhattacharya, Hrishikesh Upadhyaya
OBJECTIVES: Nanotechnology-based drug delivery systems can resolve the poor bioavailability issue allied with curcumin. The therapeutic potential of curcumin can be enhanced by making nanocomposite preparation of curcumin with metal oxide nanoparticles, poly lactic-co-glycolic acid (PLGA) nanoparticles and solid lipid nanoparticles that increases its bioavailability in the tissue. KEY FINDINGS: Curcumin has manifold therapeutic effects which include antidiabetic, antihypertensive, anticancer, anti-inflammatory and antimicrobial properties...
October 17, 2016: Journal of Pharmacy and Pharmacology
Ghislain Djiokeng Paka, Charles Ramassamy
One major challenge in the field of nanotherapeutics is to increase the selective delivery of cargo to targeted cells. Using Poly Lactic-co-Glycolic Acid (PLGA), we recently highlighted the importance of polymer composition in the biological fate of the nanodrug delivery systems. However the route of internalisation of polymeric nanoparticles (NPs) is another key component to consider in the elaboration of modern and targeted devices. For that purpose, herein, we effectively synthesized and characterised glutathione- functionalized PLGA-nanoparticles (GSH-NPs) loaded with curcumin (GSH-NPs-Cur), using thiol-maleimide click reaction and determined their physicochemical properties...
October 15, 2016: Molecular Pharmaceutics
Nazende Günday Türeli, Akif Emre Türeli, Marc Schneider
Design of Experiments (DoE) is a powerful tool for systematic evaluation of process parameters' effect on nanoparticle (NP) quality with minimum number of experiments. DoE was employed for optimization of ciprofloxacin loaded PLGA NPs for pulmonary delivery against Pseudomonas aeruginosa infections in cystic fibrosis (CF) lungs. Since the biofilm produced by bacteria was shown to be a complicated 3D barrier with heterogeneous meshes ranging from 100nm to 500 nm, nanoformulations small enough to travel through those channels were assigned as target quality...
October 12, 2016: International Journal of Pharmaceutics
Neda Khameh Khorshid, Kaizheng Zhu, Kenneth D Knudsen, Sara Bekhradnia, Sverre Arne Sande, Bo Nyström
The thermoresponsive amphiphilic block copolymer poly(d,l-lactic acid-co-glycolic acid)-block-poly(ethylene glycol)-block-poly(d,l-lactic acid-co-glycolic acid) (PLGA-PEGn -PLGA), which exhibits a reversible temperature-induced sol-gel transition at higher polymer concentrations in aqueous solution has attached a great deal of interest because of its potential in biomedical applications. In the present work, the length of the hydrophobic PLGA blocks is kept constant, whereas the length of the hydrophilic PEG block is altered and this variation has a pronounced impact on the phase behavior of the aqueous samples and the structure of the polymer...
October 14, 2016: Macromolecular Bioscience
Serban San-Marina, Ayushman Sharma, Stephen G Voss, Jeffrey R Janus, Grant S Hamilton
Importance: Nasal reconstruction in patients who are missing a significant amount of structural nasal support remains a difficult challenge. One challenge is the deficiency of cartilage left within the nose as a consequence of rhinectomy or a midline destructive disease. Historically, the standard donor source for large quantities of native cartilage has been costal cartilage. Objective: To enable the development of protocols for new mesenchymal stem cell technologies as alternative procedures with reduced donor site morbidity, risk of infection and extrusion...
October 13, 2016: JAMA Facial Plastic Surgery
Sanaul Mustafa, V Kusum Devi, Roopa S Pai
Kanamycin sulphate (KS) is a Mycobacterium tuberculosis protein synthesis inhibitor. KS is polycationic, a property responsible for KS poor oral absorption half-life (2.5 h), and rapid renal clearance, which results in serious nephrotoxicity/ototoxicity. The current study aimed to develop KS-loaded PLGA-Vitamin-E-TPGS microparticles (MPs) and nanoparticles (NPs) to reduce the dosing frequency and dose related adverse effect. In vitro release was sustained up to 10 days for KS PLGA-TPGS MPs and 13 days for KS PLGA-TPGS NPs in PBS pH 7...
October 13, 2016: Journal of Microencapsulation
Yuan Zhao, Meng-Lei Huan, Miao Liu, Ying Cheng, Yang Sun, Han Cui, Dao-Zhou Liu, Qi-Bing Mei, Si-Yuan Zhou
With the extensive application of doxorubicin (DOX), DOX resistance has become one of the main obstacles to the effective treatment of breast cancer. In this paper, DOX and resveratrol (RES) were co-encapsulated in a modified PLGA nanoparticle (NPS) to overcome the DOX resistance. CLSM results indicated that DOX and RES were simultaneously delivered into the nucleus of DOX-resistant human breast cancer cells by DOX/RES-loaded NPS. Consequently, DOX/RES-loaded NPS showed significant cytotoxicity on MDA-MB-231/ADR cells and MCF-7/ADR cells...
October 12, 2016: Scientific Reports
Olivera Evrova, Vahid Hosseini, Vincent Milleret, Gemma Palazzolo, Marcy Zenobi-Wong, Tullio Sulser, Johanna Buschmann, Daniel Eberli
Cellular responses are regulated by their microenvironments and engineered synthetic scaffolds can offer control over different microenvironment properties. This important relationship can be used as a tool to manipulate cell fate and cell responses for different biomedical applications. We showed for the first time in this study how blending of poly(ethylene oxide) (PEO) to poly(lactic-co-glycolic acid) (PLGA) fibers to yield hybrid scaffolds changes physical and mechanical properties of PLGA fibrous scaffolds and in turn affects cellular response...
October 11, 2016: ACS Applied Materials & Interfaces
Xiao-Yu Xiao, Ying-Xian Zhu, Ju-Yuan Bu, Guo-Wei Li, Jian-Hui Zhou, Shao-Peng Zhou
The purpose of the present study was to evaluate the neuroprotective efficacy of optimized thymoquinone loaded PLGA-chitosan nanoparticles delivered via nose to brain route in the rodent cerebral ischemia-reperfusion model. The neuroprotective efficacy of the optimized thymoquinone loaded PLGA-chitosan nanoparticles was evaluated in middle cerebral artery occluded rats by various pharmacodynamic and biochemical studies. The pharmacokinetics of thymoquinone loaded PLGA-chitosan nanoparticles in the brain and blood plasma together with qualitative localization of florescent labelled PLGA-chitosan nanoparticles in brain tissues were also determined...
2016: BioMed Research International
Xiangwei Liu, Naiwen Tan, Yuchao Zhou, Xueying Zhou, Hui Chen, Hongbo Wei, Ji Chen, Xiaoru Xu, Sijia Zhang, Guodong Yang, Yingliang Song
Adipose mesenchymal stem cells (ASCs) are considered as the promising seed cells for bone regeneration. However, the lower osteogenic differentiation capacity limits its therapeutic efficacy. Identification of the key molecules governing the differences between ASCs and BMSCs would shed light on manipulation of ASCs towards osteogenic phenotype. In this study, we screened semaphorin family members in ASCs and BMSCs and identified Sema3A as an osteogenic semaphorin that was significantly and predominantly expressed in BMSCs...
2016: Stem Cells International
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