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https://www.readbyqxmd.com/read/29689131/unsupervised-statistically-based-systems-biology-approach-for-unraveling-the-genetics-of-complex-traits-a-demonstration-with-ethanol-metabolism
#1
Ryan Lusk, Laura M Saba, Lauren A Vanderlinden, Vaclav Zidek, Jan Silhavy, Michal Pravenec, Paula L Hoffman, Boris Tabakoff
BACKGROUND: A statistical pipeline was developed and used for determining candidate genes and candidate gene co-expression networks involved in two alcohol (i.e., ethanol) metabolism phenotypes, namely alcohol clearance and acetate area under the curve (AUC) in a recombinant inbred (HXB/BXH) rat panel. The approach was also used to provide an indication of how ethanol metabolism can impact the normal function of the identified networks. METHODS: RNA was extracted from alcohol-naïve liver tissue of 30 strains of HXB/BXH recombinant inbred rats...
April 24, 2018: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/29688190/-present-day-possibilities-of-non-invasive-control-over-microcirculation-and-metabolism-in-man
#2
A A Fedorovich, A G Bagdasarian, I G Uchkin, G N Soboleva, S A Boĭtsov
The main function of the microcirculatory bed consists in maintaining tissue homeostasis at an optimal level irrespective of the effect of various external and internal factors. Of all types of metabolism (diffusive, filtration-reabsorption and vesicular), directly dependent on the haemodynamic parameters is filtration-reabsorption metabolism which provides exchange of water, low-molecular-weight and water-soluble substances at the opposite to the heart «pole» of the cardiovascular system. The present study was aimed at testing a hypothesis that activation of metabolic processes in man would be accompanied by alterations in haemodynamic parameters which may be registered by means of modern non-invasive methods of examination, i...
2018: Angiologii︠a︡ i Sosudistai︠a︡ Khirurgii︠a︡, Angiology and Vascular Surgery
https://www.readbyqxmd.com/read/29683664/assessment-of-the-acute-toxicity-uptake-and-biotransformation-potential-of-benzotriazoles-in-zebrafish-danio-rerio-larvae-combining-hilic-with-rplc-hrms-for-high-throughput-identification
#3
Dimitrios E Damalas, Anna A Bletsou, Adamantia Agalou, Dimitris Beis, Nikolaos S Thomaidis
The current study reports on the toxicity, uptake and the biotransformation potential of zebrafish (embryos and larvae) exposed to Benzotriazoles (BTs). Acute toxicity assays were conducted. Cardiac function abnormalities (pericardial edema and poor blood circulation) were observed from the phenotypic analysis of early life zebrafish embryos after BTs exposure. For the uptake and biotransformation experiment, extracts of whole body larvae were analyzed using liquid chromatography-high-resolution tandem mass spectrometry (UPLC-Q-TOF-HRMS/MS)...
April 23, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29683439/perfusable-vascular-network-with-a-tissue-model-in-a-microfluidic-device
#4
Yuji Nashimoto, Yukako Teraoka, Ramin Banan Sadeghian, Akiko Nakamasu, Yuichiro Arima, Sanshiro Hanada, Hidetoshi Kotera, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa
A spheroid (a multicellular aggregate) is regarded as a good model of living tissues in the human body. Despite the significant advancement in the spheroid cultures, a perfusable vascular network in the spheroids remains a critical challenge for long-term culture required to maintain and develop their functions, such as protein expressions and morphogenesis. The protocol presents a novel method to integrate a perfusable vascular network within the spheroid in a microfluidic device. To induce a perfusable vascular network in the spheroid, angiogenic sprouts connected to microchannels were guided to the spheroid by utilizing angiogenic factors from human lung fibroblasts cultured in the spheroid...
April 4, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29677833/convenient-colorimetric-detection-of-cholesterol-using-multi-enzyme-co-incorporated-organic-inorganic-hybrid-nanoflowers
#5
Minsoo Chung, Yoon Jung Jang, Moon Il Kim
We have developed a microscale well-plate colorimetric assay for the multiplexed detection of cholesterol in clinical human blood samples. This system utilizes a novel multi-enzyme incorporated organic-inorganic hybrid nanoflower which entrap both cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) as the organic components with copper phosphate as the inorganic component to detect cholesterol levels in blood samples. The hybrid nanoflowers, synthesized via an extremely simple but rapid sonication-mediated method within 5 min at room temperature, enable an efficient one-pot two-enzyme cascade reaction...
September 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29670899/guava-leaf-extract-diminishes-hyperglycemia-and-oxidative-stress-prevents-%C3%AE-cell-death-inhibits-inflammation-and-regulates-nf-kb-signaling-pathway-in-stz-induced-diabetic-rats
#6
Muthukumaran Jayachandran, Ramachandran Vinayagam, Ranga Rao Ambati, Baojun Xu, Stephen Sum Man Chung
Traditional Chinese medication has been utilized by Chinese medical practitioners to treat the varied symptoms of diabetes mellitus (DM). Notably, guava leaf has been used to treat diabetes in Asia. Our present study has been designed to analyze the action of guava leaf extract (GLE) at the molecular level in treating DM. A low dose of streptozotocin (STZ) was used to induce experimental diabetes in animals. Rats were treated with GLE at different concentrations (100, 200, and 400 mg/kg b.w.). The standard drug glibenclamide (GB) (600  μ g/kg b...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29664114/superior-real-time-polymerase-chain-reaction-detection-of-babesia-microti-parasites-in-whole-blood-utilizing-high-copy-bmn-antigens-as-amplification-targets
#7
Bryan Grabias, Jean Clement, Peter Krause, Timothy Lepore, Sanjai Kumar
BACKGROUND: Babesiosis is a zoonotic disease transmitted to humans by the bite of infected ticks and caused by apicomplexan parasites, most commonly Babesia microti. Additionally, blood and blood products collected from asymptomatically infected blood donors may cause transfusion-transmitted infections in recipients. Highly sensitive molecular assays that detect parasite nucleic acid are needed for laboratory diagnosis and to identify and defer clinically silent but parasitemic blood donors...
April 17, 2018: Transfusion
https://www.readbyqxmd.com/read/29660629/fatty-acid-profile-of-blood-plasma-and-oviduct-and-uterine-fluid-during-early-and-late-luteal-phase-in-the-horse
#8
B Drews, V Milojevic, K Giller, S E Ulbrich
During early pregnancy, the secretome of both oviduct and uterus serves as exchange medium for signaling factors between embryo and mother and provides the embryo with nutrients. The preimplantation embryo can utilize the fatty acids (FA) therein via direct incorporation into cell membrane lipid bilayers and for energy production via β-oxidation. The FA concentration and composition of the oviduct (OF) and uterine fluid (UF) might be regulated by ovarian hormones to meet the changing needs of the growing embryo...
April 7, 2018: Theriogenology
https://www.readbyqxmd.com/read/29659562/organic-cation-transporter-1-oct1-modulates-multiple-cardiometabolic-traits-through-effects-on-hepatic-thiamine-content
#9
Xiaomin Liang, Sook Wah Yee, Huan-Chieh Chien, Eugene C Chen, Qi Luo, Ling Zou, Meiling Piao, Arias Mifune, Ligong Chen, Meredith E Calvert, Sarah King, Frode Norheim, Janna Abad, Ronald M Krauss, Kathleen M Giacomini
A constellation of metabolic disorders, including obesity, dysregulated lipids, and elevations in blood glucose levels, has been associated with cardiovascular disease and diabetes. Analysis of data from recently published genome-wide association studies (GWAS) demonstrated that reduced-function polymorphisms in the organic cation transporter, OCT1 (SLC22A1), are significantly associated with higher total cholesterol, low-density lipoprotein (LDL) cholesterol, and triglyceride (TG) levels and an increased risk for type 2 diabetes mellitus, yet the mechanism linking OCT1 to these metabolic traits remains puzzling...
April 16, 2018: PLoS Biology
https://www.readbyqxmd.com/read/29658985/using-rotational-thromboelastometry-clot-firmness-at-5-minutes-rotem-%C3%A2-extem-a5-to-predict-massive-transfusion-and-in-hospital-mortality-in-trauma-a-retrospective-analysis-of-1146-patients
#10
J M Kelly, S Rizoli, P Veigas, S Hollands, A Min
Viscoelastic assays such as TEG® and ROTEM® are increasingly used to guide transfusion of blood products. The EXTEM assay maximum clot firmness (MCF) is a ROTEM measure available after 25-29 min used to guide early decisions. EXTEM A10, the clot firmness at 10 min, is an accepted early surrogate, but investigators differ on whether A5, the clot firmness at 5 min, is acceptable. We re-examined this in a retrospective observational analysis of 1146 trauma patients in one centre who had ROTEM data recorded. A5 and A10 both correlated well with maximum clot firmness, with Pearson coefficients of r = 0...
April 16, 2018: Anaesthesia
https://www.readbyqxmd.com/read/29655564/feeding-reduced-fat-dried-distillers-grains-with-solubles-to-lactating-holstein-dairy-cows-does-not-alter-milk-composition-or-cause-late-blowing-in-cheese
#11
E D Testroet, D C Beitz, M R O'Neil, A L Mueller, H A Ramirez-Ramirez, S Clark
Feeding dried distillers grains with solubles (DDGS) to lactating dairy cows has been implicated as a cause of late blowing defects in the production of Swiss-style cheeses. Our objectives were (1) to test the effect of feeding reduced-fat DDGS (RF-DDGS; ∼6% fat) to lactating dairy cows on the composition of milk and on the suitability of the milk for production of baby Swiss cheese and (2) to evaluate the effect of diet on cow lactation performance. Lactating Holstein dairy cows were fed both dietary treatments in a 2 × 2 crossover design...
April 11, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29652685/blood-patch-in-a-jehovah-s-witness-case-report-of-a-novel-arterial-to-epidural-closed-circuit-technique
#12
Kevin R Olsen, Ashley L Screws, Stephen O Vose
Jehovah's Witness patients have unique perioperative challenges involving blood products. We describe the use of a novel method to maintain a closed circuit between a Jehovah's Witness patient's arterial blood and the epidural space while performing a blood patch for postdural puncture headache. Previously described methods have utilized venous catheters to maintain a closed circuit between the body and the epidural space. This is the first report we are aware of that utilizes a closed-circuit arterial blood supply to create an epidural blood patch in a Jehovah's Witness patient...
April 15, 2018: A&A practice
https://www.readbyqxmd.com/read/29652276/utility-of-thromboelastography-versus-routine-coagulation-tests-for-assessment-of-hypocoagulable-state-in-patients-undergoing-cardiac-bypass-surgery
#13
Seema Sharma, Sujeet Kumar, Prabhat Tewari, Shantanu Pande, Manjula Murari
Introduction: Peri-operative monitoring of coagulation is important to diagnose potential cause of hemorrhage, to manage coagulopathy and guide treatment with blood products in patients undergoing cardiac surgery with cardiopulmonary bypass. This study was done to evaluate usefulness of Thromboelastography (TEG) and routine coagulation tests (RCT) in assessing hemostatic changes and predicting postoperative bleeding in patients undergoing cardiac surgery with cardiopulmonary bypass. Methods: Fifty adult patients undergoing cardiac surgery with cardiopulmonary bypass were enrolled in this prospective study...
April 2018: Annals of Cardiac Anaesthesia
https://www.readbyqxmd.com/read/29629338/metabolic-symbiosis-and-immunomodulation-how-tumor-cell-derived-lactate-may-disturb-innate-and-adaptive-immune-responses
#14
REVIEW
Alexandre Morrot, Leonardo Marques da Fonseca, Eduardo J Salustiano, Luciana Boffoni Gentile, Luciana Conde, Alessandra Almeida Filardy, Tatiany Nunes Franklim, Kelli Monteiro da Costa, Celio Geraldo Freire-de-Lima, Leonardo Freire-de-Lima
The tumor microenvironment (TME) is composed by cellular and non-cellular components. Examples include the following: (i) bone marrow-derived inflammatory cells, (ii) fibroblasts, (iii) blood vessels, (iv) immune cells, and (v) extracellular matrix components. In most cases, this combination of components may result in an inhospitable environment, in which a significant retrenchment in nutrients and oxygen considerably disturbs cell metabolism. Cancer cells are characterized by an enhanced uptake and utilization of glucose, a phenomenon described by Otto Warburg over 90 years ago...
2018: Frontiers in Oncology
https://www.readbyqxmd.com/read/29626699/therapeutics-incorporating-blood-constituents
#15
REVIEW
Phapanin Charoenphol, Katie Oswalt, Corey J Bishop
Blood deficiency and dysfunctionality can result in adverse events, which can primarily be treated by transfusion of blood or the re-introduction of properly functioning sub-components. Blood constituents can be engineered on the sub-cellular (i.e., DNA recombinant technology) and cellular level (i.e., cellular hitchhiking for drug delivery) for supplementing and enhancing therapeutic efficacy, in addition to rectifying dysfunctioning mechanisms (i.e., clotting). Herein, we report the progress of blood-based therapeutics, with an emphasis on recent applications of blood transfusion, blood cell-based therapies and biomimetic carriers...
April 4, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29626589/red-onion-scales-ameliorated-streptozotocin-induced-diabetes-and-diabetic-nephropathy-in-wistar-rats-in-relation-to-their-metabolite-fingerprint
#16
Tarek Kamal Abouzed, María Del Mar Contreras, Kadry Mohamed Sadek, Moustafa Shukry, Doaa Abdel Hady, Wael Mohamed Gouda, Walied Abdo, Nasr Elsayed Nasr, Reham Hassan Mekky, Antonio Segura-Carretero, Khaled Abdel-Aleim Kahilo, Essam Abdel-Sattar
AIM: The present study was designed to investigate the effect of red onion scales extract (ROS) against diabetic nephropathy, in relation to its metabolic profiling. METHODS: Four groups of male Wistar rats were assigned as follows; 1st untreated group, 2nd group (animals with diabetes) treated with streptozotocin (STZ, 50mg/kg) IP, 3rd group co-treated with ROS (150mg/kg + STZ, 50mg/kg) and 4th group co-treated with ROS by a dose (300mg/kg + STZ, 50mg/kg) daily...
April 4, 2018: Diabetes Research and Clinical Practice
https://www.readbyqxmd.com/read/29617642/the-science-and-translation-of-lactate-shuttle-theory
#17
REVIEW
George A Brooks
Once thought to be a waste product of anaerobic metabolism, lactate is now known to form continuously under aerobic conditions. Shuttling between producer and consumer cells fulfills at least three purposes for lactate: (1) a major energy source, (2) the major gluconeogenic precursor, and (3) a signaling molecule. "Lactate shuttle" (LS) concepts describe the roles of lactate in delivery of oxidative and gluconeogenic substrates as well as in cell signaling. In medicine, it has long been recognized that the elevation of blood lactate correlates with illness or injury severity...
April 3, 2018: Cell Metabolism
https://www.readbyqxmd.com/read/29607473/emergency-blood-transfusion-in-children-in-a-tertiary-hospital-in-nigeria-indications-frequency-and-outcome
#18
B I Abhulimhen-Iyoha, Y T Israel-Aina
BACKGROUND: Blood transfusion is a life-saving procedure in paediatric practice. It is important in replacing blood volume in cases of haemorrhage or providing specific blood components as required. However, the procedure carries some risks and complications. The decision to transfuse, frequency of transfusion and the availability of safe blood and blood products are essential determinants of the success of the procedure. Hence, knowledge of the indications and rate of transfusion is important to ensure that blood for transfusion is safe and made available as at when due...
January 2018: West African Journal of Medicine
https://www.readbyqxmd.com/read/29603795/optia%C3%A2-continuous-mononuclear-collection-cmnc-system-is-a-safe-and-efficient-system-for-hematopoietic-progenitor-cells-apheresis-hpc-a-collection-and-yields-a-lower-product-hematocrit-hct-than-the-cobe%C3%A2-spectra-system-a-retrospective-study
#19
Soumya Pandey, Michele Cottler-Fox
PURPOSE: We evaluated the Optia® continuous mononuclear collection (CMNC) system for hematopoietic progenitor cell-apheresis (HPC-A) collection (Terumo BCT, Lakewood, CO) compared to the COBE® Spectra (Terumo BCT, Lakewood, CO), including both large volume leukapheresis (LVL) and non-LVL collections. METHODS: We performed a retrospective data analysis of all autologous HPC-A collections with the Optia® CMNC system (n = 93; LVL = 59, non-LVL = 34) since implementation at our institution and compared it with a similar number of concurrent collections utilizing the COBE® Spectra (n = 96; LVL = 68, non-LVL = 28)...
March 30, 2018: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/29596308/ep4-as-a-therapeutic-target-for-aggressive-human-breast-cancer
#20
REVIEW
Mousumi Majumder, Pinki Nandi, Ahmed Omar, Kingsley Chukwunonso Ugwuagbo, Peeyush K Lala
G-protein-coupled receptors (GPCRs, also called seven-transmembrane or heptahelical receptors) are a superfamily of cell surface receptor proteins that bind to many extracellular ligands and transmit signals to an intracellular guanine nucleotide-binding protein (G-protein). When a ligand binds, the receptor activates the attached G-protein by causing the exchange of Guanosine-5'-triphosphate (GTP) for guanosine diphosphate (GDP). They play a major role in many physiological functions, as well as in the pathology of many diseases, including cancer progression and metastasis...
March 29, 2018: International Journal of Molecular Sciences
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