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https://www.readbyqxmd.com/read/29050434/microbial-loads-and-antibiotic-resistance-patterns-of-staphylococcus-aureus-in-different-types-of-raw-poultry-based-meat-preparations
#1
Laura Buzón-Durán, Rosa Capita, Carlos Alonso-Calleja
The hygiene status of raw chicken-meat preparations from retail outlets in North-Western Spain was investigated. Microbial counts (aerobic plate counts (APCs), psychrotrophs, Enterobacteriaceae, fecal coliforms, enterococci, pseudomonads, fluorescent pseudomonads, yeasts and molds, and Staphylococcus aureus) were determined for minced meat, hamburgers, nuggets, white sausages, red sausages, escalope, and roll-ups. S. aureus isolates were tested for susceptibility to twenty antimicrobials of veterinary and human clinical significance (disc diffusion method, CLSI)...
September 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/29049954/application-of-carnobacterium-maltaromaticum-as-a-feed-additive-for-weaned-rabbits-to-improve-meat-microbial-quality-and-safety
#2
Amenan Prisca Koné, Juliana Maria Velez Zea, Dominic Gagné, Dany Cinq-Mars, Frédéric Guay, Linda Saucier
This study addresses the improvement of meat microbial quality by enriching the diet of farm animals with a protective culture. Weaned Grimaud rabbits were divided into two experimental groups: a control and a diet supplemented with Micocin® (Carnobacterium maltaromaticum CB1; 8Log10CFU/kg of feed). Overall, meat quality was not affected substantially by the treatment. Total Aerobic Mesophilic (TAM), Escherichia coli and other coliforms, Enterobacteriaceae, Staphylococcus aureus, Pseudomonas spp., Listeria spp...
October 2, 2017: Meat Science
https://www.readbyqxmd.com/read/29049928/developmental-changes-in-haemocyte-morphology-in-response-to-staphylococcus-aureus-and-latex-beads-in-the-beetle-tenebrio-molitor-l
#3
Arkadiusz Urbański, Zbigniew Adamski, Grzegorz Rosiński
The evolutionary success of insects is undoubtedly related to a well-functioning immune system. This is especially apparent during insect development by the adaptation of individuals to the changing risk of infection. In addition, current studies show that the insect immune system is characterized by some specificity in response to natural pathogens (for example, bacteria, viruses or fungi) and artificial challengers (for example, latex beads or nylon filaments). However, developmental changes and the specificity of immune system reactions simultaneously have not been analysed...
October 14, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29049321/determination-and-production-of-antimicrobial-compounds-by-aspergillus-clavatonanicus-strain-mj31-an-endophytic-fungus-from-mirabilis-jalapa-l-using-uplc-esi-ms-ms-and-td-gc-ms-analysis
#4
Vineet Kumar Mishra, Ajit Kumar Passari, Preeti Chandra, Vincent Vineeth Leo, Brijesh Kumar, Sivakumar Uthandi, Sugitha Thankappan, Vijai Kumar Gupta, Bhim Pratap Singh
Endophytic fungi associated with medicinal plants are reported as potent producers of diverse classes of secondary metabolites. In the present study, an endophytic fungi, Aspergillus clavatonanicus strain MJ31, exhibiting significant antimicrobial activity was isolated from roots of Mirabilis jalapa L., was identified by sequencing three nuclear genes i.e. internal transcribed spacers ribosomal RNA (ITS rRNA), 28S ribosomal RNA (28S rRNA) and translation elongation factor 1- alpha (EF 1α). Ethyl acetate extract of strain MJ31displayed significant antimicrobial potential against Bacillus subtilis, followed by Micrococccus luteus and Staphylococcus aureus with minimum inhibitory concentrations (MIC) of 0...
2017: PloS One
https://www.readbyqxmd.com/read/29048353/p2x-receptor-antagonists-inhibit-the-interaction-of-s-aureus-hemolysin-a-with-membranes
#5
Markus Schwiering, Matthias Husmann, Nadja Hellmann
The pore forming hemolysin A, Hla, is a major virulence factor of Staphylococcus aureus. Apparently, 1-2 pore(s) per cell suffice(s) to cause cell death. Accumulated experimental evidence points towards a major role of ATP-gated purinergic receptors (P2XR) for hemolysis caused by Hla, complement and other pore forming proteins, presumably by increasing membrane permeability. Indeed, in experiments employing rabbit erythrocytes, inhibitory concentrations of frequently employed P2XR-antagonists were in a similar range as previously reported for erythrocytes of other species and other toxins...
October 19, 2017: Toxins
https://www.readbyqxmd.com/read/29048299/blood-culture-negative-endocarditis-morocco
#6
Najma Boudebouch, M'hammed Sarih, Abdelfattah Chakib, Salma Fadili, Drissi Boumzebra, Zahira Zouizra, Badie Azamane Mahadji, Hamid Amarouch, Didier Raoult, Pierre-Edouard Fournier
We investigated the microorganisms causing blood culture-negative endocarditis (BCNE) in Morocco. We tested 19 patients with BCNE by serologic methods, molecular methods, or both and identified Bartonella quintana, Staphylococcus aureus, Streptococcus equi, and Streptococcus oralis in 4 patients. These results highlight the role of these zoonotic agents in BCNE in Morocco.
November 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29046869/staphylococcus-aureus-alpha-toxin-induces-the-formation-of-dynamic-tubules-labeled-with-lc3-within-host-cells-in-a-rab7-and-rab1b-dependent-manner
#7
María M López de Armentia, María C Gauron, María I Colombo
Staphylococcus aureus is a pathogen that causes severe infectious diseases that eventually lead to septic and toxic shock. S. aureus infection is characterized by the production of virulence factors, including enzymes and toxins. After internalization S. aureus resides in a phagosome labeled with Rab7 protein. Here, we show that S. aureus generates tubular structures marked with the small GTPases Rab1b and Rab7 and by the autophagic protein LC3 at early times post-infection. As shown by live cell imaging these tubular structures are highly dynamic, extend, branch and grow in length...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29046782/assessment-of-the-potential-for-resistance-to-antimicrobial-violet-blue-light-in-staphylococcus-aureus
#8
Rachael M Tomb, Michelle Maclean, John E Coia, Scott J MacGregor, John G Anderson
BACKGROUND: Antimicrobial violet-blue light in the region of 405 nm is emerging as an alternative technology for hospital decontamination and clinical treatment. The mechanism of action is the excitation of endogenous porphyrins within exposed microorganisms, resulting in ROS generation, oxidative damage and cell death. Although resistance to 405 nm light is not thought likely, little evidence has been published to support this. This study was designed to establish if there is potential for tolerance development, using the nosocomial pathogen Staphylococcus aureus as the model organism...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29046671/generation-of-persister-cells-of-pseudomonas-aeruginosa-and-staphylococcus-aureus-by-chemical-treatment-and-evaluation-of-their-susceptibility-to-membrane-targeting-agents
#9
Lucia Grassi, Mariagrazia Di Luca, Giuseppantonio Maisetta, Andrea C Rinaldi, Semih Esin, Andrej Trampuz, Giovanna Batoni
Persister cells (PCs) are a subset of dormant, phenotypic variants of regular bacteria, highly tolerant to antibiotics. Generation of PCs in vivo may account for the recalcitrance of most chronic infections to antimicrobial treatment and demands for the identification of new antimicrobial agents able to target such cells. The present study explored the possibility to obtain in vitro PCs of Pseudomonas aeruginosa and Staphylococcus aureus at high efficiency through chemical treatment, and to test their susceptibility to structurally different antimicrobial peptides (AMPs) and two clinically used peptide-based antibiotics, colistin and daptomycin...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29046665/boeravinone-b-a-novel-dual-inhibitor-of-nora-bacterial-efflux-pump-of-staphylococcus-aureus-and-human-p-glycoprotein-reduces-the-biofilm-formation-and-intracellular-invasion-of-bacteria
#10
Samsher Singh, Nitin P Kalia, Prashant Joshi, Ajay Kumar, Parduman R Sharma, Ashok Kumar, Sandip B Bharate, Inshad A Khan
This study elucidated the role of boeravinone B, a NorA multidrug efflux pump inhibitor, in biofilm inhibition. The effects of boeravinone B plus ciprofloxacin, a NorA substrate, were evaluated in NorA-overexpressing, wild-type, and knocked-out Staphylococcus aureus (SA-1199B, SA-1199, and SA-K1758, respectively). The mechanism of action was confirmed using the ethidium bromide accumulation and efflux assay. The role of boeravinone B as a human P-glycoprotein (P-gp) inhibitor was examined in the LS-180 (colon cancer) cell line...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29046369/structural-and-functional-studies-of-sav0551-from-staphylococcus-aureus-as-a-chaperone-and-glyoxalase-iii
#11
Hyo Jung Kim, Ki-Young Lee, Ae-Ran Kwon, Bong-Jin Lee
The DJ-1/ThiJ/PfpI superfamily of proteins is highly conserved across all biological kingdoms showing divergent multi-functions, such as chaperone, catalase, protease, and kinase. The common theme of these functions is responding to and managing various cellular stresses. DJ-1/ThiJ/PfpI superfamily members are classified into three subfamilies according to their quaternary structure (DJ-1-, YhbO-, and Hsp-types). The Hsp-type subfamily includes Hsp31, a chaperone and glyoxalase III. SAV0551, an Hsp-type subfamily member from Staphylococcus aureus, is a hypothetical protein that is predicted as Hsp31...
October 18, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29046259/assessing-the-impact-of-quality-improvement-measures-on-catheter-related-blood-stream-infections-and-catheter-salvage-experience-from-a-national-intestinal-failure-unit
#12
A Bond, A Teubner, M Taylor, C Cawley, A Abraham, M Dibb, P R Chadwick, M Soop, G Carlson, S Lal
BACKGROUND & AIMS: Prevention of catheter related blood stream infections (CRBSI) and salvage of infected central venous catheters (CVC) are vital to maintaining long term venous access in patients needing home parenteral nutrition (HPN). It remains unclear as to whether patients are best trained for catheter care at home or in hospital or whether CRBSIs are lower if the patient self-cares for the CVC. Furthermore, there is minimal data on the longer term outcome following salvage of infected catheter and limited consensus on agreed protocols for catheter salvage...
October 10, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/29046035/-laboratorial-alteration-preceding-staphylococcal-infection-clinical-manifestations-after-intestinal-transplantation
#13
Wilma Carvalho Neves Forte, Alliny Oliveira Carvalho Galvan, Pedro Salem Molino, Thais Roque, Tainá Mosca, Wangles Soler
BACKGROUND: Hospital-acquired infection, often with Staphylococcus aureus, is an important complication in intestinal transplant. CLINICAL CASE: A 2-year-old girl underwent small bowel transplantation owing to a small bowel volvulus. On the first postoperative day, lymphocyte phenotypes, serum immunoglobulins and chemotactic and phagocytic activity of neutrophils were assessed in peripheral blood. A decrease in the ingestion phase of phagocytosis by neutrophils was identified, in comparison with the results of 20 healthy children...
July 2017: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
https://www.readbyqxmd.com/read/29045693/pharmacokinetics-of-ceftaroline-in-a-preterm-infant-with-methicillin-resistant-staphylococcus-aureus-pneumonia
#14
Sara N Salerno, Janice Bernhardt, Matthew Laughon, Ravi Jhaveri, Matthew Massaro, Daniel Gonzalez
We report here the first pharmacokinetic-pharmacodynamic relationship for ceftaroline in a preterm infant born at <28 weeks' gestational age who was given ceftaroline (8.5 mg/kg every 8 hours) for pneumonia attributable to methicillin-resistant Staphyloccocus aureus. This dose of ceftaroline was adequate to achieve the pharmacodynamic endpoint associated with efficacy for methicillin-resistant Staphyloccocus aureus.
October 16, 2017: Journal of the Pediatric Infectious Diseases Society
https://www.readbyqxmd.com/read/29045648/staphylococcus-aureus-bloodstream-infections-in-latin-america-results-of-a-multinational-prospective-cohort-study
#15
Carlos Seas, Coralith Garcia, Mauro J Salles, Jaime Labarca, Carlos Luna, Carlos Alvarez-Moreno, Carlos Mejía-Villatoro, Jeannete Zurita, Manuel Guzmán-Blanco, Eduardo Rodríguez-Noriega, Jinnethe Reyes, Cesar A Arias, Cesar Carcamo, Eduardo Gotuzzo
Background: Substantial heterogeneity in the epidemiology and management of Staphylococcus aureus bacteraemia (SAB) occurs in Latin America. We conducted a prospective cohort study in 24 hospitals from nine Latin American countries. Objectives: To assess the clinical impact of SAB in Latin America. Patients and methods: We evaluated differences in the 30 day attributable mortality among patients with SAB due to MRSA compared with MSSA involving 84 days of follow-up...
October 17, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29045009/trends-in-non-lactation-breast-abscesses-in-a-tertiary-hospital-setting
#16
Apoorva Saboo, Ian Bennett
BACKGROUND: The aim of this paper was to retrospectively review non-lactation breast abscesses treated in Princess Alexandra Hospital over a 10-year period and to illustrate the trends in size, risk factors, microbiological profile and management. METHODS: A computerized search of the medical records was undertaken to identify all adult patients with a diagnosis of breast abscesses during June 2005-June 2015. Patients concurrently breastfeeding were excluded. A retrospectively review of the clinical records was performed...
October 18, 2017: ANZ Journal of Surgery
https://www.readbyqxmd.com/read/29044318/antibiotic-activity-of-plectranthus-ornatus-codd-a-traditional-medicinal-plant
#17
Fernanda R Nascimento, Kamylla R S Albuquerque, Marcos R Oliveira, Virginia R Pizziolo, Beatriz G Brasileiro, Gaspar Diaz, Marisa A N Diaz
The dichloromethane extract of Plectranthus ornatus Codd., a tradicional medicinal plant, showed antibiotic activity with minimum inhibitory concentration (MIC) values of 0.4 mg.mL-1 and 100 percent of biofilm inhibition against Staphylococcus aureus strains isolated from animals with mastitis infections. Based on these antibacterial activities, in addition to ethnopharmacological reports from healing men and farmers in Brazil, an herbal soap was produced from this active extract and was tested both in vitro and in vivo...
October 16, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/29043959/purified-citritin-in-combination-with-vancomycin-inhibits-vre-in-vitro-and-in-vivo
#18
Kléber de Sousa Oliveira, Paulo Roberto Martins Queiroz, Isabel Cristina Marques Fensterseifer, Ludovico Migliolo, Aline Lima Oliveira, Octávio Luiz Franco
Gram-positive pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecium (VRE) have been frequently associated with bacterial resistance mechanisms. These mechanisms, in turn, restrict a range of therapeutic opportunities for the treatment of infections caused by these micro-organisms. Faced with this problem, the present study aims to isolate and characterize molecules with antimicrobial activity derived from the fungus Penicillium citrinum isolated from Cerrado soil...
October 18, 2017: Microbiology
https://www.readbyqxmd.com/read/29043218/evaluation-of-antioxidant-and-antimicrobial-activities-of-ethanol-extracts-of-three-kinds-of-strawberries
#19
Semeneh Seleshe, Jong Seok Lee, Sarah Lee, Hye Jin Lee, Ga Ryun Kim, Joohong Yeo, Jong Yea Kim, Suk Nam Kang
The antioxidant and antimicrobial activities of three kinds of strawberry ethanol extracts from Robus corchorifolius L. f. (RCL), Rubus parvifolius L. var. parvifolius (RPL), and Duchesnea chrysantha Miq. (DCM) were investigated. The RPL was highest (P<0.05) in phenolic, flavonoid, and anthocyanin contents. 2,2-Diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activities of RPL and DCM extracts were higher than that of RCL (P<0.05). Hydrogen peroxide scavenging activity of RPL was high compared to DCM and RCL (P<0...
September 2017: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/29043217/antioxidant-and-antimicrobial-activities-of-quinoa-chenopodium-quinoa-willd-seeds-cultivated-in-korea
#20
Jin Hwa Park, Yun Jin Lee, Yeon Ho Kim, Ki Sun Yoon
The objective of this study was to investigate the antioxidant and antimicrobial properties of quinoa cultivated in Korea and to compare it with imported quinoa from the USA and Peru. The highest amount of total flavonoid contents (TFC) with 20.91 mg quercetin equivalents/100 g was measured in quinoa seed extract cultivated in Korea, while the total phenolic contents (TPC) were significantly higher in quinoa from the USA (16.28 mg gallic acid equivalents/100 g). In addition, quinoa extracts cultivated in Korea displayed a superior antioxidant ability in both, ferric reducing antioxidant power and 1,1-diphenyl-2-picrylhydrazyl values...
September 2017: Preventive Nutrition and Food Science
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