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Rhinovirus challenge model

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https://www.readbyqxmd.com/read/28912760/immunization-with-live-human-rhinovirus-hrv-16-induces-protection-in-cotton-rats-against-hrv14-infection
#1
Mira C Patel, Lioubov M Pletneva, Marina S Boukhvalova, Stefanie N Vogel, Adriana E Kajon, Jorge C G Blanco
Human rhinoviruses (HRVs) are the main cause of cold-like illnesses, and currently no vaccine or antiviral therapies against HRVs are available to prevent or mitigate HRV infection. There are more than 150 antigenically heterogeneous HRV serotypes, with ∼90 HRVs belonging to major group species A and B. Development of small animal models that are susceptible to infection with major group HRVs would be beneficial for vaccine research. Previously, we showed that the cotton rat (Sigmodon hispidus) is semi-permissive to HRV16 (major group, species HRV-A virus) infection, replicating in the upper and lower respiratory tracts with measurable pathology, mucus production, and expression of inflammatory mediators...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28397284/altered-fatty-acid-metabolism-and-reduced-stearoyl-coenzyme-a-desaturase-activity-in-asthma
#2
N Rodriguez-Perez, E Schiavi, R Frei, R Ferstl, P Wawrzyniak, S Smolinska, M Sokolowska, N A Sievi, M Kohler, P Schmid-Grendelmeier, D Michalovich, K D Simpson, E M Hessel, M Jutel, M Martin-Fontecha, O Palomares, C A Akdis, L O'Mahony
BACKGROUND: Fatty acids and lipid mediator signaling play an important role in the pathogenesis of asthma, yet this area remains largely under-explored. The aims of this study were (i) to examine fatty acid levels and their metabolism in obese and non-obese asthma patients and (ii) to determine the functional effects of altered fatty acid metabolism in experimental models. METHODS: Medium- and long-chain fatty acid levels were quantified in serum from 161 human volunteers by LC/MS...
April 11, 2017: Allergy
https://www.readbyqxmd.com/read/28343401/effect-of-probiotic-on-innate-inflammatory-response-and-viral-shedding-in-experimental-rhinovirus-infection-a-randomised-controlled-trial
#3
R B Turner, J A Woodfolk, L Borish, J W Steinke, J T Patrie, L M Muehling, S Lahtinen, M J Lehtinen
Ingestion of probiotics appears to have modest effects on the incidence of viral respiratory infection. The mechanism of these effects is not clear; however, there is evidence from animal models that the probiotic may have an effect on innate immune responses to pathogens. The purpose of this randomised, placebo-controlled study was to determine the effect of administration of Bifidobacterium animalis subspecies lactis Bl-04 on innate and adaptive host responses to experimental rhinovirus challenge. The effect on the response of chemokine (C-X-C motif) ligand 8 (CXCL8) to rhinovirus infection was defined as the primary endpoint for the study...
March 27, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28166307/picornavirus-rna-is-protected-from-cleavage-by-ribonuclease-during-virion-uncoating-and-transfer-across-cellular-and-model-membranes
#4
Elisabetta Groppelli, Hazel C Levy, Eileen Sun, Mike Strauss, Clare Nicol, Sarah Gold, Xiaowei Zhuang, Tobias J Tuthill, James M Hogle, David J Rowlands
Picornaviruses are non-enveloped RNA viruses that enter cells via receptor-mediated endocytosis. Because they lack an envelope, picornaviruses face the challenge of delivering their RNA genomes across the membrane of the endocytic vesicle into the cytoplasm to initiate infection. Currently, the mechanism of genome release and translocation across membranes remains poorly understood. Within the enterovirus genus, poliovirus, rhinovirus 2, and rhinovirus 16 have been proposed to release their genomes across intact endosomal membranes through virally induced pores, whereas one study has proposed that rhinovirus 14 releases its RNA following disruption of endosomal membranes...
February 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/27936016/a-tool-for-investigating-asthma-and-copd-exacerbations-a-newly-manufactured-and-well-characterised-gmp-wild-type-human-rhinovirus-for-use-in-the-human-viral-challenge-model
#5
RANDOMIZED CONTROLLED TRIAL
Daniel J Fullen, Bryan Murray, Julie Mori, Andrew Catchpole, Daryl W Borley, Edward J Murray, Ganesh Balaratnam, Anthony Gilbert, Alex Mann, Fiona Hughes, Rob Lambkin-Williams
BACKGROUND: Human Rhinovirus infection is an important precursor to asthma and chronic obstructive pulmonary disease exacerbations and the Human Viral Challenge model may provide a powerful tool in studying these and other chronic respiratory diseases. In this study we have reported the production and human characterisation of a new Wild-Type HRV-16 challenge virus produced specifically for this purpose. METHODS AND STOCK DEVELOPMENT: A HRV-16 isolate from an 18 year old experimentally infected healthy female volunteer (University of Virginia Children's Hospital, USA) was obtained with appropriate medical history and consent...
2016: PloS One
https://www.readbyqxmd.com/read/27853256/mucosal-vaccination-with-a-live-recombinant-rhinovirus-followed-by-intradermal-dna-administration-elicits-potent-and-protective-hiv-specific-immune-responses
#6
Khamis Tomusange, Danushka Wijesundara, Jason Gummow, Steve Wesselingh, Andreas Suhrbier, Eric J Gowans, Branka Grubor-Bauk
Mucosal immunity is deemed crucial to control sexual transmission of human immunodeficiency virus (HIV). Herein we report the efficacy of a mucosal HIV vaccine strategy comprising intranasal (IN) vaccination with a cocktail of live recombinant human rhinoviruses (HRVs) encoding overlapping fragments of HIV Gag and full length Tat (rHRV-Gag/Tat) followed by intradermal (ID) vaccination with DNA vaccines encoding HIV Gag and Tat (pVAX-Gag-Tat). This heterologous prime-boost strategy will be referred to hereafter as rHRV-DNA...
November 17, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27836899/trail-signaling-is-proinflammatory-and-proviral-in-a-murine-model-of-rhinovirus-1b-infection
#7
Jason L Girkin, Luke M Hatchwell, Adam M Collison, Malcolm R Starkey, Philip M Hansbro, Hideo Yagita, Paul S Foster, Joerg Mattes
the aim of this study is to elucidate the role of TRAIL during rhinovirus (RV) infection in vivo. Naïve wild-type and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-deficient (Tnfsf10(-/-)) BALB/c mice were infected intranasally with RV1B. In separate experiments, Tnfsf10(-/-) mice were sensitized and challenged via the airway route with house dust mite (HDM) to induce allergic airways disease and then challenged with RVIB or UV-RVIB. Airway hyperreactivity (AHR) was invasively assessed as total airways resistance in response to increasing methacholine challenge and inflammation was assessed in bronchoalveolar lavage fluid at multiple time points postinfection...
January 1, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/27664815/invited-essay-cognitive-influences-on-the-psychological-immune-system
#8
REVIEW
S J Rachman
The construct of the psychological immune system is described and analysed. The direct and indirect cognitive influences on the system are discussed, and the implications of adding a cognitive construal to the influential model of a behavioural immune system are considered. The psychological immune system has two main properties: defensive and healing. It encompasses a good amount of health-related phenomena that is outside the scope of the behavioural model or the biological immune system. Evidence pertaining to the psychological immune system includes meta-analyses of the associations between psychological variables such as positive affect/wellbeing and diseases and mortality, and associations between wellbeing and positive health...
December 2016: Journal of Behavior Therapy and Experimental Psychiatry
https://www.readbyqxmd.com/read/27583193/il-1%C3%AE-mediates-cellular-cross-talk-in-the-airway-epithelial-mesenchymal-trophic-unit
#9
Alison R Hill, Jessica E Donaldson, Cornelia Blume, Natalie Smithers, Liku Tezera, Kamran Tariq, Patrick Dennison, Hitasha Rupani, Matthew J Edwards, Peter H Howarth, Christopher Grainge, Donna E Davies, Emily J Swindle
The bronchial epithelium and underlying fibroblasts form an epithelial mesenchymal trophic unit (EMTU) which controls the airway microenvironment. We hypothesized that cell-cell communication within the EMTU propagates and amplifies the innate immune response to respiratory viral infections. EMTU co-culture models incorporating polarized (16HBE14o-) or differentiated primary human bronchial epithelial cells (HBECs) and fibroblasts were challenged with double-stranded RNA (dsRNA) or rhinovirus. In the polarized EMTU model, dsRNA affected ionic but not macromolecular permeability or cell viability...
July 2016: Tissue Barriers
https://www.readbyqxmd.com/read/27348296/rhinovirus-infection-in-murine-chronic-allergic-rhinosinusitis-model
#10
Sung Bu Lee, Jeong-Ah Song, Go-Eun Choi, Hun Sik Kim, Yong Ju Jang
BACKGROUND: Patients with chronic rhinosinusitis (CRS) commonly experience aggravation of their symptoms after viral upper respiratory infection (URI). Rhinovirus (RV) is the most common URI-causing virus. However, there is a lack of a mouse model of RV infection and in vivo studies investigating the effect of RV infection on CRS. METHODS: A mouse model of chronic allergic rhinosinusitis (CARS) was established by sensitizing to ovalbumin (OVA) through intraperitoneal injection followed by nasal challenges with OVA for 5 weeks...
June 27, 2016: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/26947980/leveraging-gene-environment-interactions-and-endotypes-for-asthma-gene-discovery
#11
REVIEW
Klaus Bønnelykke, Carole Ober
Asthma is a heterogeneous clinical syndrome that includes subtypes of disease with different underlying causes and disease mechanisms. Asthma is caused by a complex interaction between genes and environmental exposures; early-life exposures in particular play an important role. Asthma is also heritable, and a number of susceptibility variants have been discovered in genome-wide association studies, although the known risk alleles explain only a small proportion of the heritability. In this review, we present evidence supporting the hypothesis that focusing on more specific asthma phenotypes, such as childhood asthma with severe exacerbations, and on relevant exposures that are involved in gene-environment interactions (GEIs), such as rhinovirus infections, will improve detection of asthma genes and our understanding of the underlying mechanisms...
March 2016: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/26322544/effect-of-live-and-inactivated-lactobacillus-rhamnosus-gg-on-experimentally-induced-rhinovirus-colds-randomised-double-blind-placebo-controlled-pilot-trial
#12
RANDOMIZED CONTROLLED TRIAL
M Kumpu, R A Kekkonen, R Korpela, S Tynkkynen, S Järvenpää, H Kautiainen, E K Allen, J O Hendley, A Pitkäranta, B Winther
The aim of this work was to investigate the usability of an experimental rhinovirus model in probiotic trials aiming to assess effectiveness in viral infections, and to provide preliminary data of live and inactivated probiotic Lactobacillus rhamnosus GG for larger-scale trials utilising the model. 59 subjects were randomised to receive 100 ml of fruit juice supplemented with 10(9) cfu of live or heat-inactivated (by spray-drying) L. rhamnosus GG or control juice daily for six weeks. After three weeks subjects were intranasally inoculated with experimental rhinovirus...
2015: Beneficial Microbes
https://www.readbyqxmd.com/read/26108570/toll-like-receptor-7-governs-interferon-and-inflammatory-responses-to-rhinovirus-and-is-suppressed-by-il-5-induced-lung-eosinophilia
#13
Luke Hatchwell, Adam Collison, Jason Girkin, Kristy Parsons, Junyao Li, Jie Zhang, Simon Phipps, Darryl Knight, Nathan W Bartlett, Sebastian L Johnston, Paul S Foster, Peter A B Wark, Joerg Mattes
BACKGROUND: Asthma exacerbations represent a significant disease burden and are commonly caused by rhinovirus (RV), which is sensed by Toll-like receptors (TLR) such as TLR7. Some asthmatics have impaired interferon (IFN) responses to RV, but the underlying mechanisms of this clinically relevant observation are poorly understood. OBJECTIVES: To investigate the importance of intact TLR7 signalling in vivo during RV exacerbation using mouse models of house dust mite (HDM)-induced allergic airways disease exacerbated by a superimposed RV infection...
September 2015: Thorax
https://www.readbyqxmd.com/read/25706956/phenotypic-responses-of-differentiated-asthmatic-human-airway-epithelial-cultures-to-rhinovirus
#14
Jianwu Bai, Steven L Smock, George R Jackson, Kenzie D MacIsaac, Yongsheng Huang, Courtney Mankus, Jonathan Oldach, Brian Roberts, Yu-Lu Ma, Joel A Klappenbach, Michael A Crackower, Stephen E Alves, Patrick J Hayden
OBJECTIVES: Human airway epithelial cells are the principal target of human rhinovirus (HRV), a common cold pathogen that triggers the majority of asthma exacerbations. The objectives of this study were 1) to evaluate an in vitro air liquid interface cultured human airway epithelial cell model for HRV infection, and 2) to identify gene expression patterns associated with asthma intrinsically and/or after HRV infection using this model. METHODS: Air-liquid interface (ALI) human airway epithelial cell cultures were prepared from 6 asthmatic and 6 non-asthmatic donors...
2015: PloS One
https://www.readbyqxmd.com/read/25328560/prophylactic-antibody-treatment-and-intramuscular-immunization-reduce-infectious-human-rhinovirus-16-load-in-the-lower-respiratory-tract-of-challenged-cotton-rats
#15
Jorge C G Blanco, Susan Core, Lioubov M Pletneva, Thomas H March, Marina S Boukhvalova, Adriana E Kajon
Human rhinoviruses (HRV) represent the single most important etiological agents of the common cold and are the most frequent cause of acute respiratory infections in humans. Currently the performance of available animal models for immunization studies using HRV challenge is very limited. The cotton rat (Sigmodon hispidus) is a well-recognized model for the study of human respiratory viral infections. In this work we show that, without requiring any genetic modification of either the host or the virus, intranasal infection of cotton rats with HRV16 resulted in measurable lower respiratory tract pathology, mucus production, and expression of interferon-activated genes...
2014: Trials in Vaccinology
https://www.readbyqxmd.com/read/24907978/macrophage-activation-state-determines-the-response-to-rhinovirus-infection-in-a-mouse-model-of-allergic-asthma
#16
Jun Young Hong, Yutein Chung, Jessica Steenrod, Qiang Chen, Jing Lei, Adam T Comstock, Adam M Goldsmith, J Kelley Bentley, Uma S Sajjan, Marc B Hershenson
BACKGROUND: The mechanisms by which viruses cause asthma exacerbations are not precisely known. Previously, we showed that, in ovalbumin (OVA)-sensitized and -challenged mice with allergic airway inflammation, rhinovirus (RV) infection increases type 2 cytokine production from alternatively-activated (M2) airway macrophages, enhancing eosinophilic inflammation and airways hyperresponsiveness. In this paper, we tested the hypothesis that IL-4 signaling determines the state of macrophage activation and pattern of RV-induced exacerbation in mice with allergic airways disease...
2014: Respiratory Research
https://www.readbyqxmd.com/read/24655063/small-interfering-rnas-targeted-to-interleukin-4-and-respiratory-syncytial-virus-reduce-airway-inflammation-in-a-mouse-model-of-virus-induced-asthma-exacerbation
#17
Musa R Khaitov, Igor P Shilovskiy, Aleksandra A Nikonova, Nadezda N Shershakova, Oleg Y Kamyshnikov, Alexander A Babakhin, Vitaly V Zverev, Sebastian L Johnston, Rakhim M Khaitov
Asthma exacerbations are caused primarily by viral infections. Antisense and small interfering RNA (siRNA) technologies have gained attention as potential antiasthma and antiviral approaches. In this study we analyzed whether gene silencing of interleukin (IL)-4 expression and respiratory syncytial virus (RSV) replication by RNA interference is able to suppress allergen- and virus-induced responses in a mouse model of virus-induced asthma exacerbation. Knockdown efficacy of IL-4 siRNA molecules was analyzed in the human HEK293T cell line by cotransfection of six different siRNAs with a plasmid carrying mouse IL-4...
July 2014: Human Gene Therapy
https://www.readbyqxmd.com/read/24632596/rhinovirus-exacerbates-house-dust-mite-induced-lung-disease-in-adult-mice
#18
Jennifer A Phan, Anthony Kicic, Luke J Berry, Lynette B Fernandes, Graeme R Zosky, Peter D Sly, Alexander N Larcombe
Human rhinovirus is a key viral trigger for asthma exacerbations. To date, murine studies investigating rhinovirus-induced exacerbation of allergic airways disease have employed systemic sensitisation/intranasal challenge with ovalbumin. In this study, we combined human-rhinovirus infection with a clinically relevant mouse model of aero-allergen exposure using house-dust-mite in an attempt to more accurately understand the links between human-rhinovirus infection and exacerbations of asthma. Adult BALB/c mice were intranasally exposed to low-dose house-dust-mite (or vehicle) daily for 10 days...
2014: PloS One
https://www.readbyqxmd.com/read/24471509/comparison-of-viral-load-in-individuals-with-and-without-asthma-during-infections-with-rhinovirus
#19
COMPARATIVE STUDY
Joshua L Kennedy, Marcus Shaker, Victoria McMeen, James Gern, Holliday Carper, Deborah Murphy, Wai-Ming Lee, Yury A Bochkov, Rose F Vrtis, Thomas Platts-Mills, James Patrie, Larry Borish, John W Steinke, William A Woods, Peter W Heymann
RATIONALE: Most virus-induced attacks of asthma are caused by rhinoviruses (RVs). OBJECTIVES: To determine whether people with asthma are susceptible to an increased viral load during RV infection. METHODS: Seventy-four children (4-18 yr old) were enrolled; 28 with wheezing, 32 with acute rhinitis, and 14 without respiratory tract symptoms. Nasal washes were evaluated using quantitative polymerase chain reaction for RV to judge viral load along with gene sequencing to identify strains of RV...
March 1, 2014: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/24388637/salmeterol-attenuates-chemotactic-responses-in-rhinovirus-induced-exacerbation-of-allergic-airways-disease-by-modulating-protein-phosphatase-2a
#20
Luke Hatchwell, Jason Girkin, Matthew D Dun, Matthew Morten, Nicole Verrills, Hamish D Toop, Jonathan C Morris, Sebastian L Johnston, Paul S Foster, Adam Collison, Joerg Mattes
BACKGROUND: β-Agonists are used for relief and control of asthma symptoms by reversing bronchoconstriction. They might also have anti-inflammatory properties, but the underpinning mechanisms remain poorly understood. Recently, a direct interaction between formoterol and protein phosphatase 2A (PP2A) has been described in vitro. OBJECTIVE: We sought to elucidate the molecular mechanisms by which β-agonists exert anti-inflammatory effects in allergen-driven and rhinovirus 1B-exacerbated allergic airways disease (AAD)...
June 2014: Journal of Allergy and Clinical Immunology
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