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Asthma challenge model

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https://www.readbyqxmd.com/read/28629359/forced-expiration-measurements-in-mouse-models-of-obstructive-and-restrictive-lung-diseases
#1
Fien C Devos, André Maaske, Annette Robichaud, Lore Pollaris, Sven Seys, Carolina Aznar Lopez, Erik Verbeken, Matthias Tenbusch, Rik Lories, Benoit Nemery, Peter Hm Hoet, Jeroen Aj Vanoirbeek
BACKGROUND: Pulmonary function measurements are important when studying respiratory disease models. Both resistance and compliance have been used to assess lung function in mice. Yet, it is not always clear how these parameters relate to forced expiration (FE)-related parameters, most commonly used in humans. We aimed to characterize FE measurements in four well-established mouse models of lung diseases. METHOD: Detailed respiratory mechanics and FE measurements were assessed concurrently in Balb/c mice, using the forced oscillation and negative pressure-driven forced expiration techniques, respectively...
June 19, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28626774/adenosine-triphosphate-promotes-allergen-induced-airway-inflammation-and-th17-cell-polarization-in-neutrophilic-asthma
#2
Fang Zhang, Xin Su, Gang Huang, Xiao-Feng Xin, E-Hong Cao, Yi Shi, Yong Song
Adenosine triphosphate (ATP) is a key mediator to alert the immune dysfunction by acting on P2 receptors. Here, we found that allergen challenge caused an increase of ATP secretion in a murine model of neutrophilic asthma, which correlated well with neutrophil counts and interleukin-17 production. When ATP signaling was blocked by intratracheal administration of the ATP receptor antagonist suramin before challenge, neutrophilic airway inflammation, airway hyperresponsiveness, and Th17-type responses were reduced significantly...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28624607/obese-asthmatics-have-decreased-surfactant-protein-a-levels-mechanisms-and-implications
#3
Njira Lugogo, Dave Francisco, Kenneth J Addison, Akarsh Manne, William Pederson, Jennifer L Ingram, Cynthia L Green, Benjamin T Suratt, James J Lee, Mary E Sunday, Monica Kraft, Julie G Ledford
BACKGROUND: Eosinophils are prominent in some individuals with asthma and are elevated in the submucosa in a subgroup of obese asthmatics. Surfactant protein-A (SP-A) modulates host responses to infectious and environmental insults. OBJECTIVE: To determine if SP-A levels are altered in obese asthma patients compared to a control group and to determine the implications of these alterations in SP-A levels in asthma. METHODS: Bronchoalveolar lavage fluid from 23 lean, 12 overweight and 20 obese subjects were examined for SP-A...
June 14, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28624362/pulvis-fellis-suis-extract-attenuates-ovalbumin-induced-airway-inflammation-in-murine-model-of-asthma
#4
Jiao He, Le Lv, Ziye Wang, Chuanchuan Huo, Zongwei Zheng, Bing Yin, Peizhi Jiang, Yang Yang, Jing Li, Yuan Gao, Jing Xue
ETHNOPHARMACOLOGICAL RELEVANCE: Pulvis Fellis Suis (PFS), named with "Zhu Danfen" in China, has been extensively used for the therapy of enteritis, acute pharyngitis, whooping cough and asthma in folk medicine. Although PFS shows anti-inflammatory activities, its effect on airway inflammation in asthma has not been studied. AIM OF THE STUDY: To explore the protective effect of PFS ethanol extract against airway inflammation in asthmatic mice. MATERIALS AND METHODS: Allergic asthma in mice was sensitized and challenged by OVA...
June 14, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28606589/regulatory-effects-of-interleukin-il-15-on-allergen-induced-airway-obstruction
#5
Sathisha Upparahalli Venkateshaiah, Xiang Zhu, Priya Rajavelu, Rituraj Niranjan, Murli Manohar, Alok K Verma, Joseph A Lasky, Anil Mishra
BACKGROUND: Airway obstruction is a physiologic feature of asthma and IL-15 may have an important role in the pathogenesis of asthma. OBJECTIVE: We tested the hypothesis that regulation of IL-15 is critical for the preservation of allergen-induced airway hyperresponsiveness (AHR), airway resistance and compliance in response to methacholine. METHODS: Airway inflammation, AHR, resistance and compliance were assessed in IL-15- gene deficient mice and IL-15 overexpressing mice in an allergen-induced murine model of asthma...
June 9, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28606240/-effects-of-vasoactive-intestinal-peptide-on-airway-inflammation-and-th17-treg-balance-in-asthmatic-mice
#6
Li-Qin Ke, Feng-Mei Wang, Yun-Chun Luo
OBJECTIVE: To investigate the effects of vasoactive intestinal peptide (VIP) on the airway inflammation and its regulatory effect on Th17/Treg imbalance in asthmatic mice. METHODS: A total of 30 BALB/c mice were equally and randomly divided into three groups: control, asthma, and VIP. An acute asthmatic mouse model was established by sensitization and challenge with ovalbumin (OVA). The control group received normal saline instead of OVA. Before the challenge with OVA, the VIP group was administered VIP (20 μg/mL) by aerosol inhalation for 30 minutes...
June 2017: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/28586774/the-use-of-mouse-asthma-models-to-successfully-discover-and-develop-novel-drugs
#7
Michelle M Epstein, Cornelia Tilp, Klaus J Erb
The past 20 years have seen a proliferation of scientific data on the pathophysiology of asthma. Most of these data were generated in mice using tool reagents, gene-deficient or transgenic animals. In contrast, studies on disease pathogenesis in patients are scarce. Previously, a good novel antiasthma target for drug development was one that abrogated asthma in mice when it was knocked out, neutralized or induced asthma when it was overexpressed. This type of approach led to many drug candidates that worked in mice but unfortunately failed in patients, thereby demonstrating that the results of experiments in mice are not always predictive of clinical efficacy...
June 7, 2017: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/28584561/active-ingredients-of-epimedii-folium-and-ligustri-lucidi-fructus-balanced-gr-hsp90-to-improve-the-sensitivity-of-asthmatic-rats-to-budesonide
#8
Xiufeng Tang, Xiaoxi Li, Honglei Nian, Yan Yang, Yuheng Chen, Xiujuan Wang, Liping Xu, Xinwei Yang, Renhui Liu
This study aimed to investigate the possible molecular mechanisms of active ingredients of Epimedii Folium (EF) and Ligustri Lucidi Fructus (LLF) combined with Budesonide (Bun) in asthmatic rats. Rats were divided into 5 groups, including normal group, asthma model group, Bun group, group of active ingredients of EL and LLF (EL), and group of coadministration of Bun with EL (Bun&EL). The asthmatic model was prepared by ovalbumin sensitizing and challenging. Lymphocyte apoptosis, GR protein and binding, and the protein and mRNA of GRα, GRβ, and HSP90 were tested...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28581024/blomia-tropicalis-allergen-5-blo-t-5-t-cell-epitopes-and-their-ability-to-suppress-the-allergic-immune-response
#9
Kenneth H Wong, Qian Zhou, Nayana Prabhu, Kazuki Furuhashi, Yen Leong Chua, Gijsbert M Grotenbreg, David M Kemeny
Blomia tropicalis is the major asthma allergen in the tropics comparable to Dermatophagoides pteronyssinus. However, little is known about the B tropicalis epitopes recognized by T cells. Our aim was to identify the T cell epitopes in the major B tropicalis allergen, Blo t 5, and investigate the potential of the corresponding peptides to inhibit the allergic inflammatory lung response. C57BL/6 mice were immunized with plasmid DNA encoding Blo t 5 and T cell epitopes identified using the IFN-γ ELISPOT assay with 15-mer overlapping peptides...
June 5, 2017: Immunology
https://www.readbyqxmd.com/read/28576975/route-of-administration-affects-corticosteroid-sensitivity-of-a-combined-ovalbumin-and-lipopolysaccharide-model-of-asthma-exacerbation-in-guinea-pigs
#10
Alexander P P Lowe, Rhian S Thomas, Anthony T Nials, Emma J Kidd, Kenneth J Broadley, William R Ford
Lipopolysaccharide (LPS) contributes to asthma exacerbations and development of inhaled corticosteroid insensitivity. Complete resistance to systemic corticosteroids is rare and most patients lie on a continuum of steroid responsiveness. The objective of this study was to examine the sensitivity of combined ovalbumin- (Ova) and LPS-induced functional and inflammatory responses to inhaled and systemic corticosteroid in conscious guinea-pigs, to test the hypothesis that the route of administration affects its sensitivity...
June 2, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/28570992/inhalation-of-concentrated-pm2-5-from-mexico-city-acts-as-an-adjuvant-in-a-guinea-pig-model-of-allergic-asthma
#11
Carlos Iván Falcon-Rodriguez, Andrea De Vizcaya-Ruiz, Irma Aurora Rosas-Pérez, Álvaro Román Osornio-Vargas, Patricia Segura-Medina
Exposure to Particulate Matter (PM) could function as an adjuvant depending on the city of origin in mice allergic asthma models. Therefore, our aim was to determine whether inhalation of fine particles (PM2.5) from Mexico City could act as an adjuvant inducing allergic sensitization and/or worsening the asthmatic response in guinea pig, as a suitable model of human asthma. Experimental groups were Non-Sensitized (NS group), sensitized with Ovalbumin (OVA) plus Aluminum hydroxide (Al(OH)3) as adjuvant (S + Adj group), and sensitized (OVA) without adjuvant (S group)...
May 29, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28566688/sublingual-house-dust-mite-immunotherapy-has-no-impact-on-decrease-of-circulating-erythrocytes-upon-airway-allergen-challenge-in-allergic-rhinitis
#12
Galateja Jordakieva, Michael Kundi, Patrick Lemell, René Zieglmayer, Petra Zieglmayer, Jasminka Godnic-Cvar, Erika Jensen-Jarolim
House dust mite (HDM) allergy is a predominant cause for perennial allergic rhinitis (AR) in Europe. We recently reported that circulating erythrocyte numbers decrease after airway allergen challenge in a murine asthma model and in grass-pollen sensitized AR subjects. Consequently, we aimed to evaluate these findings in HDM sensitized AR subjects and the influence of preceding allergen immunotherapy. Seventy-seven (age 26.8 ± 7.3 years; 54.5% female) HDM-allergic rhinitis subjects previously enrolled in a randomized, monocentric sublingual immunotherapy (SLIT) trial at the Vienna Challenge Chamber (VCC) were included...
May 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28559992/delivery-of-adipose-derived-mesenchymal-stem-cells-attenuates-airway-responsiveness-and-inflammation-in-a-mouse-model-of-ovalbumin-induced-asthma
#13
Ranran Dai, Jia Liu, Songbai Cai, Chengxiao Zheng, Xin Zhou
Adipose-derived mesenchymal stem cells (ADMSCs) possess immunomodulation property, yet their therapeutic potential in asthma is unclear. This study aimed to explore the effects of ADMSCs on airway hyperresponsiveness and inflammation in ovalbumin (OVA)-induced asthma models. The underlying mechanism(s) was also examined. BALB/c mice were sensitized with OVA on days 0, 7, and 14, followed by 8-week OVA challenge from day 22. ADMSCs were injected via tail vein on day 21. Animals were measured for airway responsiveness, lung pathology, IgE and cytokine levels in serum, cell composition in bronchoalveolar lavage fluid (BALF), gene expression in the lung, and regulatory T cells (Tregs)...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28555089/stigmasterol-modulates-allergic-airway-inflammation-in-guinea-pig-model-of-ovalbumin-induced-asthma
#14
Aaron Opoku Antwi, David Darko Obiri, Newman Osafo
We explored the potential benefits of stigmasterol in the treatment of asthma, an airway disorder characterized by immune pathophysiology and with an ever-increasing worldwide prevalence. We assessed the modulatory effect of the intraperitoneal administration of stigmasterol on experimentally induced airway inflammation in guinea pigs. The effect of stigmasterol on inflammatory cell proliferation, oxidative stress, lung histopathology, and remodeling was investigated. The results showed significant suppressive effects on ovalbumin-induced airway inflammatory damage...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28551494/isoimperatorin-attenuates-airway-inflammation-and-mucus-hypersecretion-in-an-ovalbumin-induced-murine-model-of-asthma
#15
Charith U B Wijerathne, Chang-Seob Seo, Ji-Won Song, Hee-Seon Park, Og-Sung Moon, Young-Suk Won, Hyo-Jung Kwon, Hwa-Young Son
Isoimperatorin (IMP), an active natural furocoumarin, has numerous pharmacologic effects, including anti-inflammatory, analgesic, antispasmodic, and anticancer activities. This study aimed to evaluate the preventive activity of IMP in an ovalbumin (OVA)-induced murine model of asthma and to investigate its possible molecular mechanisms. Female BALB/c mice were sensitized on days 0 and 14 via intraperitoneal injection of 20μg OVA. On days 21-23 after the initial sensitization, the mice received an airway challenge with OVA (1% w/v in PBS) for 1h; meanwhile, IMP (10 or 30mg/kg once daily) was administered by gavage on days 18-23...
May 25, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28543436/prenatal-environmental-tobacco-smoke-exposure-increases-allergic-asthma-risk-with-methylation-changes-in-mice
#16
Sonja Christensen, Zeina Jaffar, Elizabeth Cole, Virginia Porter, Maria Ferrini, Britten Postma, Kent E Pinkerton, Mihi Yang, Yang Jee Kim, Luke Montrose, Kevan Roberts, Andrij Holian, Yoon Hee Cho
Allergic asthma remains an inadequately understood disease. In utero exposure to environmental tobacco smoke (ETS) has been identified as an environmental exposure that can increase an individual's asthma risk. To improve our understanding of asthma onset and development, we examined the effect of in utero ETS exposure on allergic disease susceptibility in an asthmatic phenotype using a house dust mite (HDM) allergen-induced murine model. Pregnant C57BL/6 mice were exposed to either filtered air or ETS during gestation, and their offspring were further exposed to HDM at 6-7 weeks old to induce allergic inflammation...
May 25, 2017: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/28516728/increased-heterogeneity-of-airway-calibre-in-adult-rats-after-hypoxia-induced-intrauterine-growth-restriction
#17
Kimberley C W Wang, Jude S Morton, Sandra T Davidge, Alexander N Larcombe, Alan L James, Graham M Donovan, Peter B Noble
BACKGROUND AND OBJECTIVE: Intrauterine growth restriction (IUGR) is associated with asthma development. We hypothesized that IUGR disrupts airway development leading to postnatal structural abnormalities of the airway that predispose to disease. This study therefore examined structural changes to the airway and lung in a rat model of maternal hypoxia-induced IUGR. METHODS: Pregnant rats were housed under hypoxic conditions (11.5% O2 ) from gestational days (GDs) 13 to 20 (pseudoglandular-canalicular stages, i...
May 17, 2017: Respirology: Official Journal of the Asian Pacific Society of Respirology
https://www.readbyqxmd.com/read/28515363/lung-cd103-dendritic-cells-restrain-allergic-airway-inflammation-through-il-12-production
#18
Laura Conejero, Sofía C Khouili, Sarai Martínez-Cano, Helena M Izquierdo, Paola Brandi, David Sancho
DCs are necessary and sufficient for induction of allergic airway inflammation. CD11b+ DCs direct the underlying Th2 immunity, but debate surrounds the function of CD103+ DCs in lung immunity and asthma after an allergic challenge. We challenged Batf3-/- mice, which lacked lung CD103+ DCs, with the relevant allergen house dust mite (HDM) as a model to ascertain their role in asthma. We show that acute and chronic HDM exposure leads to defective Th1 immunity in Batf3-deficient mice. In addition, chronic HDM challenge in Batf3-/- mice results in increased Th2 and Th17 immune responses and exacerbated airway inflammation...
May 18, 2017: JCI Insight
https://www.readbyqxmd.com/read/28507585/ctgf-upregulation-correlates-with-mmp-9-level-in-airway-remodeling-in-a%C3%A2-murine-model-of-asthma
#19
Sheng-Chieh Lin, Hsiu-Chu Chou, Bor-Luen Chiang, Chung-Ming Chen
INTRODUCTION: Connective tissue growth factor (CTGF) mediates hypertrophy, proliferation, and extracellular matrix synthesis. Matrix metalloproteinase (MMP) plays a role in airway extracellular matrix remodeling. The correlation between CTGF and MMP in airway remodeling of asthma was unknown. This study investigated lung CTGF expression and its correlation with MMP and airway structural changes in a murine model of asthma. MATERIAL AND METHODS: Female BALB/c mice were sensitized and challenged by intraperitoneal injections and intranasal phosphate-buffered saline (PBS) or ovalbumin (OVA)...
April 1, 2017: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/28500314/activation-of-eosinophils-interacting-with-bronchial-epithelial-cells-by-antimicrobial-peptide-ll-37-implications-in-allergic-asthma
#20
Delong Jiao, Chun-Kwok Wong, Miranda Sin-Man Tsang, Ida Miu-Ting Chu, Dehua Liu, Jing Zhu, Man Chu, Christopher Wai-Kei Lam
The role of antimicrobial peptide LL-37 in asthma exacerbation is unclear. Microbial infection, which is the most common inducer of asthma exacerbation, is accompanied by elevated LL-37. The present study found that co-culture of eosinophils and bronchial epithelial cell line BEAS-2B significantly enhanced intercellular adhesion molecule-1 on both cells and CD18 expression on eosinophils upon LL-37 stimulation. IL-6, CXCL8 and CCL4 were substantially released in co-culture in the presence of LL-37. LL-37 triggered the activation of eosinophils interacting with BEAS-2B cells in a P2X purinoceptor 7/epidermal growth factor receptor-dependent manner...
May 12, 2017: Scientific Reports
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