keyword
MENU ▼
Read by QxMD icon Read
search

Isolation of Lactic acid bacteria

keyword
https://www.readbyqxmd.com/read/28098755/exopolysaccharides-isolated-from-milk-fermented-with-lactic-acid-bacteria-prevent-ultraviolet-induced-skin-damage-in-hairless-mice
#1
Masashi Morifuji, Masami Kitade, Tomoyuki Fukasawa, Taketo Yamaji, Masamitsu Ichihashi
BACKGROUND: We studied the mechanism by which fermented milk ameliorates UV-B-induced skin damage and determined the active components in milk fermented with lactic acid bacteria by evaluating erythema formation, dryness, epidermal proliferation, DNA damage and cytokine mRNA levels in hairless mice exposed to acute UV-B irradiation. METHODS: Nine week-old hairless mice were given fermented milk (1.3 g/kg BW/day) or exopolysaccharide (EPS) concentrate (70 mg/kg BW/day) orally for ten days...
January 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28093322/anti-pathogenic-and-probiotic-attributes-of-lactobacillus-salivarius-and-lactobacillus-plantarum-strains-isolated-from-feces-of-algerian-infants-and-adults
#2
Hamza Ait Seddik, Farida Bendali, Benoit Cudennec, Djamel Drider
Sixty-seven (67) lactic acid bacteria (LAB) isolates belonging to Lactobacillus genus were isolated from human feces and tested for their auto-aggregation and cell surface hydrophobicity in order to establish their adhesion capabilities, a prerequisite for probiotic selection. Strains with the upmost auto-aggregation and cell surface hydrophobicity scores were identified by MALDI-TOF spectrometry and 16S rDNA sequencing as Lactobacillus plantarum (p25lb1 and p98lb1) and Lactobacillus salivarius (p85lb1 and p104lb1)...
January 13, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28092820/pcr-screening-of-an-african-fermented-pearl-millet-porridge-metagenome-to-investigate-the-nutritional-potential-of-its-microbiota
#3
Fabien Saubade, Christèle Humblot, Youna M Hemery, Jean-Pierre Guyot
Cereals are staple foods in most African countries, and many African cereal-based foods are spontaneously fermented. The nutritional quality of cereal products can be enhanced through fermentation, and traditional cereal-based fermented foods (CBFFs) are possible sources of lactic acid bacteria (LAB) with useful nutritional properties. The nutritional properties of LAB vary depending on the species and even on the strain, and the microbial composition of traditional CBFFs varies from one traditional production unit (TPU) to another...
December 29, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28070665/current-state-of-purification-isolation-and-analysis-of-bacteriocins-produced-by-lactic-acid-bacteria
#4
REVIEW
Vilma Kaškonienė, Mantas Stankevičius, Kristina Bimbiraitė-Survilienė, Gintarė Naujokaitytė, Loreta Šernienė, Kristina Mulkytė, Mindaugas Malakauskas, Audrius Maruška
The scientific interest for the search of natural means of microbial inhibitors has not faded for several years. A search of natural antibiotics, so-called bacteriocins which are produced by lactic acid bacteria (LAB), gains a huge attention of the scientists in the last century, in order to reduce the usage of synthetic food additives. Pure bacteriocins with wide spectra of antibacterial activity are promising among the natural biopreservatives. The usage of bacteriocin(s) producing LAB as starter culture for the fermentation of some food products, in order to increase their shelf-life, when synthetic preservatives are not allowable, is also possible...
January 9, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28070022/stable-isotope-profiles-reveal-active-production-of-vocs-from-human-associated-microbes
#5
Joann Phan, Simone Meinardi, Barbara Barletta, Donald Blake, Katrine Whiteson
Volatile organic compounds (VOCs) measured from exhaled breath have great promise for the diagnosis of bacterial infections. However, determining human or microbial origin of VOCs detected in breath remains a great challenge. For example, the microbial fermentation product 2,3-butanedione was recently found in the breath of CF patients; parallel culture-independent metagenomic sequencing of the same samples revealed that Streptococcus and Rothia spp. have the genetic capacity to produce 2,3-butanedione. To investigate whether the genetic capacity found in metagenomes translates to bacterial production of a VOC of interest such as 2,3-butanedione, we fed stable isotopes to three bacterial strains isolated from Cystic Fibrosis (CF) patients: two Gram-positive bacteria, Rothia mucilaginosa and Streptococcus salivarius, and a dominant opportunistic Gram-negative pathogen, Pseudomonas aeruginosa...
January 10, 2017: Journal of Breath Research
https://www.readbyqxmd.com/read/28063102/quantitative-analyses-of-the-bacterial-microbiota-of-rearing-environment-tilapia-and-common-carp-cultured-in-earthen-ponds-and-inhibitory-activity-of-its-lactic-acid-bacteria-on-fish-spoilage-and-pathogenic-bacteria
#6
Pierre Marie Kaktcham, Jules-Bocamdé Temgoua, François Ngoufack Zambou, Gloria Diaz-Ruiz, Carmen Wacher, María de Lourdes Pérez-Chabela
The present study aimed to evaluate the bacterial load of water, Nile Tilapia and common Carp intestines from earthen ponds, isolate lactic acid bacteria (LAB) and assess their antimicrobial activity against fish spoilage and pathogenic bacteria. Following enumeration and isolation of microorganisms the antimicrobial activity of the LAB isolates was evaluated. Taxonomic identification of selected antagonistic LAB strains was assessed, followed by partial characterisation of their antimicrobial metabolites. Results showed that high counts (>4 log c...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28061327/lowering-histamine-formation-in-a-red-ribera-del-duero-wine-spain-by-using-an-indigenous-o-oeni-strain-as-a-malolactic-starter
#7
Carmen Berbegal, Yaiza Benavent-Gil, Eva Navascués, Almudena Calvo, Clara Albors, Isabel Pardo, Sergi Ferrer
This study demonstrates for the first time that a non-commercial selected autochthonous O. oeni strain has been used to conduct malolactic fermentation (MLF) while lowering histamine formation in the same winery. Lactic acid bacteria (LAB) were isolated from 13 vats before and after spontaneous MLF at the Pago de Carraovejas winery from the Ribera del Duero region (Spain). Only O. oeni were present, typed and characterized, and both histamine producer and non-producers existed. From the non-producers, one strain was selected to become a starter according to its genetic profile, prevalence in the different wines in the winery, resistance to alcoholic degree, resistance to high polyphenolic content, inability to synthesise histamine, growth kinetics and malolactic activity...
December 26, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28043774/draft-genome-sequence-of-alcohol-tolerant-bacteria-pediococcus-acidilactici-strain-k3
#8
Gun-Seok Park, Sung-Jun Hong, Seulki Park, Hyewon Jin, Sang-Jae Lee, Jae-Ho Shin, Han-Seung Lee
Pediococcus acidilactici strain K3 is an alcohol-tolerant lactic acid bacterium isolated from nuruk, which is a traditional Korean fermentation starter for makgeolli brewing. Draft genome of this strain was approximately 1,991,399bp (G+C content, 42.1%) with 1525 protein-coding sequences (CDS), of which 44% were assigned to recognized functional genes. This draft genome sequence data of the strain K3 will provide insights into the genetic basis of its alcohol-tolerance.
January 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28040167/genetic-diversity-safety-and-technological-characterization-of-lactic-acid-bacteria-isolated-from-artisanal-pico-cheese
#9
M F P Domingos-Lopes, C Stanton, P R Ross, M L E Dapkevicius, C C G Silva
A total of 114 lactic acid bacteria were isolated at one and 21 days of ripening from a traditional raw cow's milk cheese without the addition of starter culture, produced by three artisanal cheese-makers in Azores Island (Pico, Portugal). Identification to species and strain level was accomplished by16S rRNA gene and PFGE analysis. Carbohydrate utilization profiles were obtained with the relevant API kits. Isolates were evaluated according to safety and technological criteria. The most frequently observed genus identified by 16S rRNA sequencing analysis was Enterococcus, whereas API system mostly identified Lactobacillus...
May 2017: Food Microbiology
https://www.readbyqxmd.com/read/28011128/isolation-of-lactic-acid-forming-bacteria-from-biogas-plants
#10
Jelena Bohn, Aytül Yüksel-Dadak, Stefan Dröge, Helmut König
Direct molecular approaches provide hints that lactic acid bacteria play an important role in the degradation process of organic material to methanogenetic substrates in biogas plants. However, their diversity in biogas fermenter samples has not been analyzed in detail yet. For that reason, five different biogas fermenters, which were fed mainly with maize silage and manure from cattle or pigs, were examined for the occurrence of lactic acid-forming bacteria. A total of 197 lactic acid-forming bacterial strains were isolated, which we assigned to 21 species, belonging to the genera Bacillus, Clostridium, Lactobacillus, Pediococcus, Streptococcus and Pseudoramibacter-related...
December 20, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/28008785/establishment-of-lactobacillus-plantarum-strain-in-honey-bee-digestive-tract-monitored-using-gfp-fluorescence
#11
P Javorský, L Kolesár Fecskeová, L Hrehová, R Sabo, J Legáth, P Pristas
Lactic acid bacteria are symbiotic bacteria that naturally reside in the gastrointestinal tract of honey bees. They serve a multitude of functions and are considered beneficial and completely harmless. In our experiments Lactobacillus plantarum strain B35, isolated from honey bee digestive tract, was modified using pAD43-25 plasmid carrying a functional GFP gene sequence (gfpmut3a) and used as a model for monitoring and optimisation of the mode of application. The establishment of this strain in honey bee digestive tract was monitored using GFP fluorescence...
December 23, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/28006700/detection-of-different-microenvironments-and-lactobacillus-sakei-biotypes-in-ventricina-a-traditional-fermented-sausage-from-central-italy
#12
Patrizio Tremonte, Elena Sorrentino, Gianfranco Pannella, Luca Tipaldi, Marina Sturchio, Armando Masucci, Lucia Maiuro, Raffaele Coppola, Mariantonietta Succi
The present study evaluated the physico-chemical and microbiological features of Ventricina, considering for the first time the presence of different compartments deriving from the technology of production. In fact meat pieces (pork muscle and fat cut into cubes of about 10-20cm(3)), mixed with other ingredients and then stuffed into pig bladder, are still distinguishable at the end of the ripening. They appear delimited on the outside by the casing and inside by thin layers consisting of spices (mainly red pepper powder), salt and meat juices...
February 2, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27988111/diagnostic-accuracy-of-a-standardized-scheme-for-identification-of-streptococcus-uberis-in-quarter-milk-samples-a-comparison-between-conventional-bacteriological-examination-modified-rambach-agar-medium-culturing-and-16s-rrna-gene-sequencing
#13
Regina Wald, Martina Baumgartner, Verena Urbantke, Beatrix Stessl, Thomas Wittek
Bacteriological examination of milk samples is a prerequisite for pathogen-specific therapy and aids in limiting antimicrobial resistance. The aims of this study were to establish a standardized scheme for reliable Streptococcus uberis identification in routine diagnosis and to evaluate the accuracy of conventional tests and growing patterns of Strep. uberis on a selective medium (modified Rambach agar medium, MRAM) using 16S rRNA gene sequencing analysis as a reference method. We obtained isolates of presumptive Strep...
December 14, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27981504/isolation-identification-and-screening-of-potential-probiotic-bacteria-in-milk-from-south-african-saanen-goats
#14
Goitsemang Makete, Olayinka A Aiyegoro, Mapitsi S Thantsha
This study aimed to evaluate lactic acid bacteria isolates from Saanen goats' milk for probiotic attributes, thereby determining their potential as direct-fed microbials for goats. Isolates were identified using API 50CH system, 16S rDNA sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry. All 17 isolates obtained were identified as Lactobacillus plantarum except one identified as Pediococcus acidilactici. Four isolates identified as L. plantarum (Accession numbers KJ026587...
December 15, 2016: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/27956866/potential-of-lactobacillus-reuteri-from-spontaneous-%C3%A2-sourdough-as-a-starter-additive-for-improving-quality-parameters-of-bread
#15
Dovilė Jonkuvienė, Lina Vaičiulytė-Funk, Joana Šalomskienė, Gitana Alenčikienė, Aldona Mieželienė
Retardation of microbial spoilage of bread can be achieved by the use of spontaneous sourdough with an antimicrobial activity. This study was undertaken to identify lactic acid bacteria naturally occurring in spontaneous sourdough and use them for quality improvement and prolonging shelf life of rye, wheat and rye with wheat bread. Identification of isolates from spontaneous sourdough by pyrosequencing assay showed that Lactobacillus reuteri were dominant lactic acid bacteria. The isolates showed a wide range of antimicrobial activity and displayed a synergistic activity against other lactobacilli, some lactococci and foodborne yeasts...
September 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27943049/functional-properties-of-lactobacillus-mucosae-strains-isolated-from-brazilian-goat-milk
#16
Georgia Maciel Dias de Moraes, Louricélia Rodrigues de Abreu, Antônio Silvio do Egito, Hévila Oliveira Salles, Liana Maria Ferreira da Silva, Luís Augusto Nero, Svetoslav Dimitrov Todorov, Karina Maria Olbrich Dos Santos
The search for probiotic candidates among lactic acid bacteria (LAB) isolated from food may uncover new strains with promising health and technological properties. Lactobacillus mucosae strains attracted recent research attention due to their ability to adhere to intestinal mucus and to inhibit pathogens in the gastrointestinal tract, both related to a probiotic potential. Properties of interest and safety aspects of three Lb. mucosae strains (CNPC006, CNPC007, and CNPC009) isolated from goat milk were investigated employing in vitro tests...
December 10, 2016: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/27936381/a-proteomic-approach-for-rapid-identification-of-weissella-species-isolated-from-korean-fermented-foods-on-maldi-tof-ms-supplemented-with-an-in-house-database
#17
Eiseul Kim, Youngjae Cho, Yoonju Lee, Sun-Kyung Han, Chang-Gyeom Kim, Dong-Won Choo, Young-Rok Kim, Hae-Yeong Kim
Weissella are obligate heterofermentative lactic acid bacteria belonging to the Leuconostocaceae family. Some Weissella can be found in salted and fermented foods, such as kimchi and jeotgal, and plays an important role in the fermentation process. In the present study, for the first time, a rapid and accurate identification method for Weissella species from kimchi and jeotgal was developed based on MALDI-TOF MS, supplemented with an in-house database. Of the 135 Weissella spectra aligned with the MALDI bioTyper database, 56 isolates (41...
December 5, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27902242/rapid-and-accurate-identification-of-pediococcus-species-isolated-from-korean-fermented-foods-by-maldi-tof-ms-with-local-database-extension
#18
Youngjae Cho, Eiseul Kim, Yoonju Lee, Sun-Kyung Han, Chang-Gyeom Kim, Dong-Won Choo, Young-Rok Kim, Hae-Yeong Kim
Pediococci are halophilic lactic acid bacteria, within the family Lactobacillaceae, which are involved in the fermentation of various salted and fermented foods, such as kimchi and jeotgal. In this study, a MALDI-TOF MS method was developed for the rapid identification of Pediococcus species. Of the 130 Pediococcus spectra aligned with the Biotyper taxonomy database, 122 isolates (93.9%) yielded log scores <1.7, which means they were not identifiable. After registering the spectra of 11 Pediococcus reference strains, all of the isolates were correctly identified, of which 84 (64...
October 27, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27896483/the-effects-of-different-carbon-sources-on-the-antifungal-activity-by-lactic-acid-bacteria
#19
Anna Toplaghaltsyan, Inga Bazukyan, Armen Trchounian
Lactobacillus rhamnosus R-2002 strain isolated from Armenian pickled cheese sample has a fungicide effect, which was carried out by the first-time designed method. The fungicide effect of LAB strain was expressed after 5 days of co-cultivation with Mucor plumbeus and 6 days of co-cultivation with Penicillium aurantioviolaceum. The growth as well as the antifungal activity of L. rhamnosus R-2002 was shown to directly depend on the nature of the medium's carbon source. The antifungal activity of this strain against both moulds was inhibited when the combination of 10 g l(-1) glucose and 10 ml l(-1) ethanol in the medium was used as a carbon source...
February 2017: Current Microbiology
https://www.readbyqxmd.com/read/27894832/development-of-a-rapid-one-step-screening-method-for-the-isolation-of-presumptive-proteolytic-enterococci
#20
Ken Graham, Rosemary Rea, Paul Simpson, Helena Stack
Enterococci show higher proteolytic activities than other lactic acid bacteria and thus have received considerable attention in scientific literature in recent years. Proteolytic enzymes of enterococci have warranted the use of some species as starter, adjuncts or protective cultures and as probiotics, while in some strains they have also been linked with virulence. Consequently, the isolation and identification of proteolytic enterococci is becoming of increasing interest and importance. However, current screening methods for proteolytic enterococci can be time consuming, requiring a two-step procedure which may take up to 96h...
January 2017: Journal of Microbiological Methods
keyword
keyword
56894
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"