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Isolation of Lactic acid bacteria

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https://www.readbyqxmd.com/read/28721852/effective-identification-of-lactobacillus-casei-group-species-genome-based-selection-of-the-gene-mutl-as-the-target-of-a-novel-multiplex-pcr-assay
#1
Benedetta Bottari, Giovanna E Felis, Elisa Salvetti, Anna Castioni, Ilenia Campedelli, Sandra Torriani, Valentina Bernini, Monica Gatti
Lactobacillus casei,Lactobacillus paracasei and Lactobacillusrhamnosus form a closely related taxonomic group (the L. casei group) within the facultatively heterofermentative lactobacilli. Strains of these species have been used for a long time as probiotics in a wide range of products, and they represent the dominant species of nonstarter lactic acid bacteria in ripened cheeses, where they contribute to flavour development. The close genetic relationship among those species, as well as the similarity of biochemical properties of the strains, hinders the development of an adequate selective method to identify these bacteria...
July 18, 2017: Microbiology
https://www.readbyqxmd.com/read/28720951/isolation-and-characterization-of-lactic-acid-bacteria-from-traditional-pickles-of-himachal-pradesh-india
#2
Monika, Savitri, Vijay Kumar, Anila Kumari, Kunzes Angmo, Tek Chand Bhalla
A total of 15 isolates of lactic acid bacteria (LAB) were isolated from traditional pickles of rural and urban areas of Himachal Pradesh. These isolates were identified as Enterococcus faecalis (7), Lactobacillus plantarum (3), Pediococcus pentosaceus (2), Leuconostoc mesenteroides (1), Lactococcus lactis (1) and Enterococcus sp. (1) on the basis of morphological, biochemical and 16S rDNA sequence analysis. All the LABs produced exopolysaccharides, have antibiotic susceptibility, and possess activity of β-galactosidase, β-glucosidase, protease and amylase, while none of the isolates showed haemolytic activity...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28720151/supplementation-with-lactobacillus-paracasei-or-pediococcus-pentosaceus-does-not-prevent-diarrhoea-in-neonatal-pigs-infected-with-escherichia-coli-f18
#3
Anders D Andersen, Malene S Cilieborg, Charlotte Lauridsen, Anne Louise Mørkbak, Per Torp Sangild
Infectious diarrhoea is a worldwide problem in newborns. Optimal bacterial colonisation may enhance gut maturation and protect against pathogenic bacteria after birth. We hypothesised that lactic acid bacteria (LAB) administration prevents pathogen-induced diarrhoea in formula-fed newborns. Newborn caesarean-delivered, colostrum-deprived term piglets on parenteral nutrition for the first 15 h, were used as models for sensitive newborn infants. A commercially available probiotic strain, Lactobacillus paracasei F19 (LAP, 2·6×108 colony-forming units (CFU)/kg per d) and a novel LAB isolate, Pediococcus pentosaceus (PEP, 1·3×1010 CFU/kg per d), were administered for 5 d with or without inoculation of the porcine pathogen, Escherichia coli F18 (F18, 1010 CFU/d)...
July 19, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28712023/selection-of-potential-probiotic-lactobacillus-with-inhibitory-activity-against-salmonella-and-fecal-coliform-bacteria
#4
Mattika Abhisingha, Jureeporn Dumnil, Chetsadaporn Pitaksutheepong
Three hundred and sixty presumptive lactic acid bacteria (LAB) isolated from pregnant sows, newborn, suckling, and weaned piglets were preliminarily screened for anti-Salmonella activity. Fifty-eight isolates consisting of Lactobacillus reuteri (n = 32), Lactobacillus salivarius (n = 10), Lactobacillus mucosae (n = 8), Lactobacillus johnsonii (n = 5), and Lactobacillus crispatus (n = 3) were selected and further characterized for probiotic properties including production of antimicrobial substances, acid and bile tolerance, and cell adherence to Caco-2 cells...
July 15, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28711259/effect-of-dairy-product-environment-on-the-growth-of-bacillus-cereus
#5
E Tirloni, E Ghelardi, F Celandroni, C Bernardi, S Stella
pH is one of the most important parameters to manage bacterial replication in foodstuffs. In this study, the ability of 2 Bacillus cereus strains, one clinical human isolate (GPe2) and one isolate from a dairy products (D43), were investigated for the in vitro growth at different pH values (from 3.5 to 7.5) at 2 temperatures (15 and 37°C), showing their ability to grow from 5.5 to 7.5 and from 5.0 to 7.5, respectively. The ability of spores of these 2 microorganisms to germinate in different typologies of dairy products (unflavored yogurt, Taleggio cheese, mascarpone cheese, and raw and pasteurized milk) was also investigated by inoculating the spores and maintaining the products at 15°C...
July 12, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28702431/isolation-of-antifungal-lactic-acid-bacteria-lab-from-kunu-against-toxigenic-aspergillus-flavus
#6
Oluwafunmilayo Oluwakemi Olonisakin, Yemisi Adefunke Jeff-Agboola, Clement Olusola Ogidi, Bamidele Juliet Akinyele
The antifungal activity of isolated lactic acid bacteria (LAB) from a locally fermented cereal, "Kunu", was tested against toxigenic Aspergillus flavus. The liquid refreshment, "Kunu", was prepared under hygienic condition using millet, sorghum, and the combination of the two grains. The antifungal potential of isolated LAB against toxigenic A. flavus was carried out using both in vitro and in vivo antifungal assays. The LAB count from prepared "Kunu" ranged from 2.80 ×10(4) CFU/mL to 4.10×10(4) CFU/mL and Lactobacillus plantarum, Lactobacillus delbrueckii, Lactobacillus fermentum, Pediococcus acidilactici, and Leuconostoc mesenteroides were the isolated bacteria...
June 2017: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/28702021/combination-of-metabolomic-and-proteomic-analysis-revealed-different-features-among-lactobacillus-delbrueckii-subspecies-bulgaricus-and-lactis-strains-while-in-vivo-testing-in-the-model-organism-caenorhabditis-elegans-highlighted-probiotic-properties
#7
Elena Zanni, Emily Schifano, Sara Motta, Fabio Sciubba, Claudio Palleschi, Pierluigi Mauri, Giuditta Perozzi, Daniela Uccelletti, Chiara Devirgiliis, Alfredo Miccheli
Lactobacillus delbrueckii represents a technologically relevant member of lactic acid bacteria, since the two subspecies bulgaricus and lactis are widely associated with fermented dairy products. In the present work, we report the characterization of two commercial strains belonging to L. delbrueckii subspecies bulgaricus, lactis and a novel strain previously isolated from a traditional fermented fresh cheese. A phenomic approach was performed by combining metabolomic and proteomic analysis of the three strains, which were subsequently supplemented as food source to the model organism Caenorhabditis elegans, with the final aim to evaluate their possible probiotic effects...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28701082/bovine-mastitis-prevention-humoral-and-cellular-response-of-dairy-cows-inoculated-with-lactic-acid-bacteria-at-the-dry-off-period
#8
M Pellegrino, N Berardo, J Giraudo, M E F Nader-Macías, C Bogni
The use of lactic acid bacteria (LAB) in animal feed, constitute an alternative tool for bovine mastitis prevention. Previously, two LAB strains were isolated from bovine milk and selected for their probiotics properties. So far, immune response of inoculating LAB in bovine udders at dry-off period has not been investigated. The immunoglobulin isotype levels and memory cell proliferation in blood and milk of animals inoculated with Lactobacillus lactis subsp. lactis CRL1655 and Lactobacillus perolens CRL1724 at dry-off period was studied...
July 13, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28698079/purification-and-fermentation-characteristics-of-exopolysaccharide-from-fomitopsis-castaneus-imaz
#9
Guo Wen-Kui, Chi Yu-Jie
Short-chain fatty acids (SCFAs), which are the end products of carbohydrate fermentation in the gut, mainly contribute to energy metabolism in mammals. The amount of SCFAs produced during fermentation is an important parameter that characterizes the fermentation capacity of a system. This paper reports on the fermentation characteristics of exopolysaccharides (EPS) isolated from Fomitopsis castaneus Imaz, a wood-rot fungal species. We isolated and purified the main EPS fraction by freeze drying and DEAE-Sepharose fast flow chromatography...
July 8, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28691342/dual-function-of-lactobacillus-kefiri-dh5-in-preventing-high-fat-diet-induced-obesity-direct-reduction-of-cholesterol-and-upregulation-of-ppar%C3%AE-in-adipose-tissue
#10
Dong-Hyeon Kim, Dana Jeong, Il-Byeong Kang, Hyunsook Kim, Kwang-Young Song, Kun-Ho Seo
SCOPE: Kefir consumption inhibits the development of obesity and non-alcoholic fatty liver disease in mice fed 60% high-fat diet (HFD). To identify the key contributor of this effect, we isolated lactic acid bacteria (LAB) from kefir and examined their anti-obesity properties from in vitro screening and in vivo validation. METHODS AND RESULTS: Thirteen kefir LAB isolates were subjected to survivability test using artificial gastrointestinal environment and cholesterol-reducing assay...
July 10, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28683528/isolation-and-characterization-of-some-promoter-sequences-from-leuconostoc-mesenteroides-sy2-isolated-from-kimchi
#11
Ji Yeong Park, Seon-Ju Jeong, Jeong A Kim, Jeong Hwan Kim
Some promoters were isolated and characterized from genome of Leuconostoc mesenteroides SY2, an isolate from kimchi, a Korean traditional fermented vegetable. Chromosomal DNA of L. mesenteroides SY2 was digested with Sau3AI and ligated with BamHI cut pBV5030, a promoter screening vector containing a promoter-less cat-86. Among E. coli transformants (TFs) resistant against Cm (chloramphenicol), 17 were able to grow in the presence of 1,000 μg/ml Cm and their inserts were sequenced. Transcription start sites were examined for 3 putative promoters (P04C, P25C, and P33C) by primer extension...
July 7, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28677149/isolating-and-evaluating-lactic-acid-bacteria-strains-for-effectiveness-on-silage-quality-at-low-temperatures-on-the-tibetan-plateau
#12
REVIEW
Siran Wang, Xianjun Yuan, Zhihao Dong, Junfeng Li, Tao Shao
Four lactic acid bacteria (LAB) strains isolated from straw silages on the Tibetan Plateau were characterized, and their effects on the fermentation quality of Italian ryegrass (Lolium multiflorum Lam.) at different temperatures (10°C, 15°C and 25°C) were studied. These LAB isolates were evaluated using the acids production ability test, morphological observation, Gram staining, physiological, biochemical and acid tolerance tests. All the isolates (M1, LM8, LO7 and LOG9) could grow at 5-20°C, pH 3.5-7.0 and NaCl (3...
July 5, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28672978/oral-administration-of-lactobacilli-isolated-from-jeotgal-a-salted-fermented-seafood-inhibits-the-development-of-2-4-dinitrofluorobenzene-induced-atopic-dermatitis-in-mice
#13
Mi-Sung Park, Nho-Eul Song, Sang-Ho Baik, Hyun-Ock Pae, Seong Hoon Park
Certain strains of lactobacilli have been reported to exert favorable effects on atopic dermatitis (AD). Jeotgal, a traditional Korean food, is a salted fermented seafood known to harbor many lactic acid bacteria. In the present study, two novel lactobacillus strains were isolated from Jeotgal, and their anti-AD effects were investigated. Lactobacilli isolated from Jeotgal were identified, according to conjugated linoleic acid-producing activity, as Lactobacillus plantarum (JBCC105645 and JBCC105683). AD-like skin lesions were induced in BALB/c mice using dinitrofluorobenzene (DNFB)...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28669141/development-of-a-new-lactic-acid-bacterial-inoculant-for-fresh-rice-straw-silage
#14
Jong Geun Kim, Jun Sang Ham, Yu Wei Li, Hyung Soo Park, Chul-Sung Huh, Byung-Chul Park
OBJECTIVE: Effects of newly isolated Lactobacillus plantarum on the fermentation and chemical composition of fresh rice straw silage was evaluated in this study. METHODS: Lactic acid bacteria (LAB) from good crop silage were screened by growing them in MRS broth and a minimal medium with low carbohydrate content. Selected LAB (LAB 1821) were Gram-positive, rods, catalase negative, and were identified to be Lactobacillus plantarum based on their biochemical characteristics and a 16S rRNA analysis...
July 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28668682/inhibition-of-herpes-simplex-virus-1-and-poliovirus-pv-1-by-bacteriocins-from-lactococcus-lactis-subsp-lactis-and-enterococcus-durans-strains-isolated-from-goat-milk
#15
Valéria Quintana Cavicchioli, Otávio Valério de Carvalho, Janine Cerqueira de Paiva, Svetoslav Dimitrov Todorov, Abelardo Silva Júnior, Luís Augusto Nero
Alternative applications of bacteriocins has been exploited due to some unusual inhibitory activity, such as their antiviral properties. Semi-purified bacteriocins of six lactic acid bacteria strains isolated from goat milk (two Lactococcus lactis: GLc03 and GLc05, and four Enterococcus durans: GEn09, GEn12, GEn14 and GEn17) were tested for cytotoxicity in Vero cells (50% Cytotoxicity Concentration: CC50), and for their antiviral activities against Herpes simplex virus 1 (HVS-1) and Poliovirus (PV-1). Semi-purified bacteriocins presented low cytotoxicity, with CC50 varying from 256...
June 28, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28659548/pathogenicity-of-enterococci
#16
Yasuyoshi Ike
Enterococci belong to the group of lactic acid bacteria (LAB), and inhabit the gastrointestinal tracts of a wide variety of animals from insects and to human, and the commensal organism in humans and animals. The commensal/probiotic role of enterococci has evolved through thousands of years in mutual coexistence. Enterococcus have many favorable traits that have been appreciated in food fermentation and preservation, and many serve as probiotics to promote health. While lactobacillus have been shown to confer numerous benefits on and often regarded as health bringing organisms, enterococci have become more recognized as emerging human pathogens in recent years...
2017: Nihon Saikingaku Zasshi. Japanese Journal of Bacteriology
https://www.readbyqxmd.com/read/28655216/phenotypic-and-genomic-survey-on-organic-acid-utilization-profile-of-pseudomonas-mendocina-strain-s5-2-a-vineyard-soil-isolate
#17
Teik Min Chong, Jian-Woon Chen, Wah-Seng See-Too, Choo-Yee Yu, Geik-Yong Ang, Yan Lue Lim, Wai-Fong Yin, Catherine Grandclément, Denis Faure, Yves Dessaux, Kok-Gan Chan
Root exudates are chemical compounds that are released from living plant roots and provide significant energy, carbon, nitrogen and phosphorus sources for microbes inhabiting the rhizosphere. The exudates shape the microflora associated with the plant, as well as influences the plant health and productivity. Therefore, a better understanding of the trophic link that is established between the plant and the associated bacteria is necessary. In this study, a comprehensive survey on the utilization of grapevine and rootstock related organic acids were conducted on a vineyard soil isolate which is Pseudomonas mendocina strain S5...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28647277/risk-factors-for-nosocomial-infections-in-patients-receiving-extracorporeal-membrane-oxygenation-supportive-therapy
#18
Geqin Sun, Binfei Li, Haili Lan, Juan Wang, Lanfei Lu, Xueqin Feng, Xihua Luo, Haizhong Yan, Yuejing Mu
BACKGROUND AND OBJECTIVE: The aim of this study was to analyze risk factors for nosocomial infection (NI) in patients receiving extracorporeal membrane oxygenation (ECMO) support. PATIENTS AND METHODS: Clinical NI data were collected from patients who received ECMO support therapy, and analyzed retrospectively. RESULTS: Among 75 ECMO patients, 20 were found to have developed NI (infection rate 26.7%); a total of 58 pathogens were isolated, including 43 strains of gram-negative bacteria (74...
June 22, 2017: Medicina Clínica
https://www.readbyqxmd.com/read/28634850/novel-vitamin-b12-producing-enterococcus-spp-and-preliminary-in-vitro-evaluation-of-probiotic-potentials
#19
Ping Li, Qing Gu, Yuejiao Wang, Yue Yu, Lanlan Yang, Jieyan V Chen
Vitamin B12 is an essential nutrient required for crucial metabolic processes in humans. Vitamin B12-producing lactic acid bacteria (LAB) have been attracting increased attentions currently because of the generally recognized as safe (GRAS) status. Most of recent studies focused on Lactobacillus, and little is known about B12-producing Enterococcus. In the present study, five Enterococcus strains isolated from infant feces were identified as vitamin B12 producers. Among them, Enterococcus faecium LZ86 had the highest B12 production (499...
June 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28626449/characterization-of-feruloyl-esterases-produced-by-the-four-lactobacillus-species-l-amylovorus-l-acidophilus-l-farciminis-and-l-fermentum-isolated-from-ensiled-corn-stover
#20
Zhenshang Xu, Huiying He, Susu Zhang, Tingting Guo, Jian Kong
Lactic acid bacteria (LAB) play important roles in silage fermentation, which depends on the production of sufficient organic acids to inhibit the growth of undesirable microorganisms. However, LAB are not able to degrade cellulose and hemicellulose. Bacteria and fibrolytic enzymes are usually used as inoculants to improve the silage quality and digestibility. In the present study, we isolated four Lactobacillus strains (L. amylovorus CGMCC 11056, L. acidophilus CCTCC AB2010208, L. farciminis CCTCC AB2016237 and L...
2017: Frontiers in Microbiology
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