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Isolation of Lactic acid bacteria

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https://www.readbyqxmd.com/read/27902242/rapid-and-accurate-identification-of-pediococcus-species-isolated-from-korean-fermented-foods-by-maldi-tof-ms-with-local-database-extension
#1
Youngjae Cho, Eiseul Kim, Yoonju Lee, Sun-Kyung Han, Chang-Gyeom Kim, Dong-Won Choo, Young-Rok Kim, Hae-Yeong Kim
Pediococci are halophilic lactic acid bacteria, within the family Lactobacillaceae, which are involved in the fermentation of various salted and fermented foods, such as kimchi and jeotgal. In this study, a MALDI-TOF MS method was developed for the rapid identification of Pediococcus species. Of the 130 Pediococcus spectra aligned with the Biotyper taxonomy database, 122 isolates (93.9%) yielded log scores <1.7, which means they were not identifiable. After registering the spectra of 11 Pediococcus reference strains, all of the isolates were correctly identified, of which 84 (64...
October 27, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27896483/the-effects-of-different-carbon-sources-on-the-antifungal-activity-by-lactic-acid-bacteria
#2
Anna Toplaghaltsyan, Inga Bazukyan, Armen Trchounian
Lactobacillus rhamnosus R-2002 strain isolated from Armenian pickled cheese sample has a fungicide effect, which was carried out by the first-time designed method. The fungicide effect of LAB strain was expressed after 5 days of co-cultivation with Mucor plumbeus and 6 days of co-cultivation with Penicillium aurantioviolaceum. The growth as well as the antifungal activity of L. rhamnosus R-2002 was shown to directly depend on the nature of the medium's carbon source. The antifungal activity of this strain against both moulds was inhibited when the combination of 10 g l(-1) glucose and 10 ml l(-1) ethanol in the medium was used as a carbon source...
November 28, 2016: Current Microbiology
https://www.readbyqxmd.com/read/27894832/development-of-a-rapid-one-step-screening-method-for-the-isolation-of-presumptive-proteolytic-enterococci
#3
Ken Graham, Rosemary Rea, Paul Simpson, Helena Stack
Enterococci show higher proteolytic activities than other lactic acid bacteria and thus have received considerable attention in scientific literature in recent years. Proteolytic enzymes of enterococci have warranted the use of some species as starter, adjuncts or protective cultures and as probiotics, while in some strains they have also been linked with virulence. Consequently, the isolation and identification of proteolytic enterococci is becoming of increasing interest and importance. However, current screening methods for proteolytic enterococci can be time consuming, requiring a two-step procedure which may take up to 96h...
November 25, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27877165/different-flour-microbial-communities-drive-to-sourdoughs-characterized-by-diverse-bacterial-strains-and-free-amino-acid-profiles
#4
Giuseppe Celano, Maria De Angelis, Fabio Minervini, Marco Gobbetti
This work aimed to investigate whether different microbial assemblies in flour may influence the microbiological and biochemical characteristics of traditional sourdough. To reach this purpose, members of lactic acid bacteria, enterobacteria, and yeasts were isolated from durum wheat flour. Secondly, the isolated microorganisms (Pediococcus pentosaceus, Saccharomyces cerevisiae, Pantoea agglomerans, and Escherichia hermannii) were inoculated in doughs prepared with irradiated flour (gamma rays at 10 kGy), so that eight different microbial assemblies were obtained...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27877164/distribution-of-native-lactic-acid-bacteria-in-wineries-of-queretaro-mexico-and-their-resistance-to-wine-like-conditions
#5
Dalia E Miranda-Castilleja, Ramón Álvar Martínez-Peniche, J A Aldrete-Tapia, Lourdes Soto-Muñoz, Montserrat H Iturriaga, J R Pacheco-Aguilar, Sofía M Arvizu-Medrano
Native lactic acid bacteria (LAB) are capable of growing during winemaking, thereby strongly affecting wine quality. The species of LAB present in musts, wines during malolactic fermentation (MLF), and barrels/filters were investigated in wineries from the emerging wine region of Queretaro, México using multiplex PCR and culture. The resistance to wine-like conditions (WLC): ethanol (10, 12, and 13%), SO2 (30 mg⋅l(-1)), and low pH (3.5) of native LAB strains was also studied. Five species were detected within 61 samples obtained: Oenococcus oeni, Lactobacillus plantarum, Pediococcus parvulus, Lactobacillus hilgardi, and Lactobacillus brevis...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27867805/isolation-of-lactic-acid-bacteria-bound-to-the-porcine-intestinal-mucosa-and-an-analysis-of-their-moonlighting-adhesins
#6
Hideki Kinoshita, Satoko Ohuchi, Kensuke Arakawa, Masamichi Watanabe, Haruki Kitazawa, Tadao Saito
The adhesion of lactic acid bacteria (LAB) to the intestinal mucosa is one of the criteria in selecting for probiotics. Eighteen LAB were isolated from porcine intestinal mucin (PIM): ten strains of Lactobacillus, six strains of Weissella, and two strains of Streptococcus. Using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) for phosphate-buffered saline (PBS) extracts from the LAB, many bands were detected in half of the samples, while a few and/or no clear bands were detected in the other half...
2016: Bioscience of Microbiota, Food and Health
https://www.readbyqxmd.com/read/27866041/study-of-the-bacterial-diversity-of-foods-pcr-dgge-versus-lh-pcr
#7
Cristiana Garofalo, Elena Bancalari, Vesna Milanović, Federica Cardinali, Andrea Osimani, Maria Luisa Savo Sardaro, Benedetta Bottari, Valentina Bernini, Lucia Aquilanti, Francesca Clementi, Erasmo Neviani, Monica Gatti
The present study compared two culture-independent methods, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) and length-heterogeneity polymerase chain reaction (LH-PCR), for their ability to reveal food bacterial microbiota. Total microbial DNA and RNA were extracted directly from fourteen fermented and unfermented foods, and domain A of the variable regions V1 and V2 of the 16S rRNA gene was analyzed through LH-PCR and PCR-DGGE. Finally, the outline of these analyses was compared with bacterial viable counts obtained after bacterial growth on suitable selective media...
November 10, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27855912/target-identification-of-volatile-metabolites-to-allow-the-differentiation-of-lactic-acid-bacteria-by-gas-chromatography-ion-mobility-spectrometry
#8
Janneth Gallegos, Cristina Arce, Rafael Jordano, Lourdes Arce, Luis M Medina
The purpose of this work was to study the potential of gas chromatography-ion mobility spectrometry (GC-IMS) to differentiate lactic acid bacteria (LAB) through target identification and fingerprints of volatile metabolites. The LAB selected were used as reference strains for their influence in the flavour of cheese. The four strains of LAB can be distinguished by the fingerprints generated by the volatile organic compounds (VOCs) emitted. 2-butanone, 2-pentanone, 2-heptanone and 3-methyl-1-butanol were identified as relevant VOCs for Lactobacillus casei and Lactobacillus paracasei subsp...
April 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27847439/lactobacillus-casei-strain-c1-attenuates-vascular-changes-in-spontaneously-hypertensive-rats
#9
Wei Boon Yap, Faisal Malau Ahmad, Yi Cheng Lim, Satirah Zainalabidin
Hypertension can be caused by various factors while the predominant causes include increase in body fluid volume and resistance in the circulatory system that elevate the blood pressure. Consumption of probiotics has been proven to attenuate hypertension; however, the effect is much strain-dependent. In this study, a newly isolated Lactobacillus casei (Lb. casei) strain C1 was investigated for its antihypertensive properties in spontaneously hypertensive rats (SHR). Lactic acid bacteria (LAB) suspension of 11 log colony-forming unit (CFU) was given to SHR (SHR+LAB, n=8), and phosphate buffer saline (PBS) was given as a control in SHR (SHR, n=8) and in Wistar rats as sham (WIS, n=8)...
November 2016: Korean Journal of Physiology & Pharmacology
https://www.readbyqxmd.com/read/27845560/lactic-acid-bacteria-isolated-from-poultry-protect-the-intestinal-epithelial-cells-of-chickens-from-in-vitro-wheat-germ-agglutinin-induced-cytotoxicity
#10
J D Babot, E Argañaraz Martínez, M J Lorenzo-Pisarello, M C Apella, A Perez Chaia
1. Poultry fed on wheat-based diets regularly ingest wheat germ agglutinin (WGA) that has toxic effects in vitro on intestinal epithelial cells (IEC) obtained from 14-d-old broilers. Cytotoxicity and the potential role of 14 intestinal bacterial strains in the removal of bound lectins in epithelial cell cultures was investigated. 2. Cytotoxicity was dependent on time and lectin concentration; the lethal dose (LD50) was 8.36 µg/ml for IEC exposed for 2 h to WGA. 2. Complementary sugars to WGA were detected on the surface of one Enterococcus and 9 Lactobacillus strains isolated from poultry...
November 15, 2016: British Poultry Science
https://www.readbyqxmd.com/read/27844338/phospholipid-polydiacetylene-vesicle-based-colorimetric-assay-for-high-throughput-screening-of-bacteriocins-and-halocins
#11
Manoj Kumar Yadav, Vijay Kumar, Bijender Singh, Santosh Kumar Tiwari
The colorimetric assay is phospholipid/polydiacetylene vesicle-based assay used for the detection of membrane-acting peptides. Bacteriocins and halocins are antimicrobial peptides known to kill target cells by membrane disruption. Therefore, the assay was applied for high-throughput (HTP) screening of bacteriocins and halocins produced by lactic acid bacteria and haloarchaea, respectively. The assay consisted of vesicles which were synthesized using four different phospholipids: dipalmitoylphosphatydilcholine (DPPC), dimyristoylphosphatidylcholine (DMPC), dimyristoylphosphoethanolamine (DMPE) and dimyristoylphosphatidylglycerol (DMPG) in combination with diacetylene monomer 10,12-tricosadiy noic acid (TRCDA)...
November 14, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27843489/long-term-adaptive-evolution-of-leuconostoc-mesenteroides-for-enhancement-of-lactic-acid-tolerance-and-production
#12
Si Yeon Ju, Jin Ho Kim, Pyung Cheon Lee
BACKGROUND: Lactic acid has been approved by the United States Food and Drug Administration as Generally Regarded As Safe (GRAS) and is commonly used in the cosmetics, pharmaceutical, and food industries. Applications of lactic acid have also emerged in the plastics industry. Lactic acid bacteria (LAB), such as Leuconostoc and Lactobacillus, are widely used as lactic acid producers for food-related and biotechnological applications. Nonetheless, industrial mass production of lactic acid in LAB is a challenge mainly because of growth inhibition caused by the end product, lactic acid...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27832440/influence-of-hydrogen-peroxide-lactic-acid-and-surfactants-from-vaginal-lactobacilli-on-the-antibiotic-sensitivity-of-opportunistic-bacteria
#13
Andrey Sgibnev, Elena Kremleva
We studied as hydrogen peroxide, lactic acid, or surfactants from clinical isolates of vaginal lactobacilli and cell-free supernatants from probiotic strain LCR35 can influence on the sensitivity of opportunistic bacteria to antibiotics. We found that the most effective in increasing sensitivity to antibiotics were hydrogen peroxide and surfactants or their combination but no lactic acid. In some cases, the effect of the composition of hydrogen peroxide and surfactants was clearly higher than the sum of effects of these substances alone...
November 10, 2016: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/27824276/identification-of-a-new-small-bioactive-peptide-from-lactobacillus-gasseri-supernatant
#14
A Zanfardino, G Criscuolo, B Di Luccia, E Pizzo, M L Ciavatta, E Notomista, A Carpentieri, A Pezzella, M Varcamonti
Ten lactic acid bacteria (LAB) strains, previously isolated from human ileal biopsy of healthy volunteers, were screened for production and secretion of molecules having anti-bacterial and anti-biofilm activities. Because many recent reports indicate that LAB secreted molecules may exert immune-modulatory action, we also tested the effect on human intestinal HCT116 cells challenged with bacterial lipopolysaccharides. One of the Lactobacillus gasseri strains, SF1109, strongly inhibited: (1) Pseudomonas aeruginosa growth; (2) Escherichia coli biofilm production; (3) LPS induction of P-ERK1/2 in HCT116 cells, and was selected for further characterisation of the secreted active molecule...
November 8, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27824273/anti-inflammaging-effects-of-lactobacillus-brevis-ow38-in-aged-mice
#15
J-J Jeong, K A Kim, Y-J Hwang, M J Han, D-H Kim
In the present study, lactic acid bacteria (LAB) strains were collected from kimchi and were screened to isolate strains that inhibit lipopolysaccharide (LPS) production by Escherichia coli and p16 expression and nuclear factor-kappa B (NF-κB) activation in LPS-stimulated macrophages. Oral administration of Lactobacillus brevis OW38 (1×10(9) cfu/mouse) to aged mice (male, 18 months old) for 8 weeks reduced the LPS level in colon fluid and blood. In addition, OW38 treatment also reduced the ratio of Firmicutes or Proteobacteria to Bacteroidetes, which was significantly higher in aged mice than in young mice...
November 8, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27816987/aspergillus-flavus-growth-inhibition-by-lactobacillus-strains-isolated-from-traditional-fermented-kenyan-milk-and-maize-products
#16
Sara Ahlberg, Vesa Joutsjoki, Sini Laurikkala, Pekka Varmanen, Hannu Korhonen
Certain strains of lactic acid bacteria have been reported to inhibit fungal growth and may so be potential as biocontrol agents. In this study, 171 LAB strains were isolated from traditional fermented Kenyan milk and maize products and tested against aflatoxin-producing A. flavus fungi. The three LAB strains showing highest antifungal activity were identified as Lactobacillus plantarum. None of the strains were able to completely inhibit fungal growth under conditions favorable for fungi and suboptimal for LAB...
November 5, 2016: Archives of Microbiology
https://www.readbyqxmd.com/read/27813217/selection-of-a-candidate-probiotic-strain-of-pediococcus-pentosaceus-from-the-faecal-microbiota-of-horses-by-in-vitro-testing-and-health-claims-in-a-mouse-model-of-salmonella-infection
#17
B C Silva, S H C Sandes, L B Alvim, M R Q Bomfim, J R Nicoli, E Neumann, A C Nunes
AIMS: The aim of this study was to verify the suitable use of candidate 'probiotics' selected by in vitro tests and the importance of in vivo assays to nominate the microorganisms as probiotics and alternatives prophylactic treatments for Salmonella Typhimurium infection. METHODS AND RESULTS: Thirty-three lactic acid bacteria (LAB) isolated from foal's faeces were assessed based on the main desirable functional in vitro criteria. Based on these results, we choosed Pediococcus pentosaceus strain #40, to evaluate its putative probiotic features in a mouse model of Salmonella infection...
November 4, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27812926/purification-and-characterization-of-a-novel-anti-campylobacter-bacteriocin-produced-by-lactobacillus-curvatus-dn317
#18
Mohamed Zommiti, Hamdan Almohammed, Mounir Ferchichi
The lactic acid bacteria (LAB) microbiota of Saudi chicken ceca was determined. From 60 samples, 204 isolates of lactic acid bacteria were obtained. Three isolates produced antimicrobial activities against Campylobacter jejuni, Listeria monocytogenes, and Bacillus subtilis. The isolate DN317, which had the highest activity against Campylobacter jejuni ATCC 33560, was identified as Lactobacillus curvatus (GenBank accession numbers: KX353849 and KX353850). Full inhibitory activity was observed after a 2-h incubation with the supernatant at pH values between 4 and 8...
November 3, 2016: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/27811103/draft-genome-sequence-of-lactobacillus-crispatus-c25-isolated-from-chicken-cecum
#19
Morvarid Rezvani, Mary Mendoza, Matthew D Koci, Caitlyn Daron, Josh Levy, Hosni M Hassan
Lactic acid bacteria are important members of the gut microbiota of humans and animals. Here, we present the genome sequence of Lactobacillus crispatus strain C25, originally isolated from the cecum of 4-week-old chicken fed a standard diet. This isolate represents a potential probiotic strain for poultry.
November 3, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27810447/improving-the-antioxidant-properties-of-quinoa-flour-through-fermentation-with-selected-autochthonous-lactic-acid-bacteria
#20
Carlo Giuseppe Rizzello, Anna Lorusso, Vito Russo, Daniela Pinto, Barbara Marzani, Marco Gobbetti
Lactic acid bacteria strains, previously isolated from the same matrix, were used to ferment quinoa flour aiming at exploiting the antioxidant potential. As in vitro determined on DPPH and ABTS radicals, the scavenging activity of water/salt-soluble extracts (WSE) from fermented doughs was significantly (P<0.05) higher than that of non-inoculated doughs. The highest inhibition of linoleic acid autoxidation was found for the quinoa dough fermented with Lactobacillus plantarum T0A10. The corresponding WSE was subjected to Reverse Phase Fast Protein Liquid Chromatography, and 32 fractions were collected and subjected to in vitro assays...
October 29, 2016: International Journal of Food Microbiology
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