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https://www.readbyqxmd.com/read/28813028/detection-methodologies-for-pathogen-and-toxins-a-review
#1
REVIEW
Md Eshrat E Alahi, Subhas Chandra Mukhopadhyay
Pathogen and toxin-contaminated foods and beverages are a major source of illnesses, even death, and have a significant economic impact worldwide. Human health is always under a potential threat, including from biological warfare, due to these dangerous pathogens. The agricultural and food production chain consists of many steps such as harvesting, handling, processing, packaging, storage, distribution, preparation, and consumption. Each step is susceptible to threats of environmental contamination or failure to safeguard the processes...
August 16, 2017: Sensors
https://www.readbyqxmd.com/read/28812060/reolysin-and-histone-deacetylase-inhibition-in-the-treatment-of-head-and-neck-squamous-cell-carcinoma
#2
Alena C Jaime-Ramirez, Jun-Ge Yu, Enrico Caserta, Ji Young Yoo, Jianying Zhang, Tae Jin Lee, Craig Hofmeister, John H Lee, Bhavna Kumar, Quintin Pan, Pawan Kumar, Robert Baiocchi, Theodoros Teknos, Flavia Pichiorri, Balveen Kaur, Matthew Old
Oncolytic viruses (OVs) are emerging as powerful anti-cancer agents and are currently being tested for their safety and efficacy in patients. Reovirus (Reolysin), a naturally occurring non-pathogenic, double-stranded RNA virus, has natural oncolytic activity and is being tested in phase I-III clinical trials in a variety of tumor types. With its recent US Food and Drug Administration (FDA) orphan drug designation for several tumor types, Reolysin is a potential therapeutic agent for various cancers, including head and neck squamous cell carcinomas (HNSCCs), which have a 5-year survival of ∼55%...
June 16, 2017: Molecular Therapy Oncolytics
https://www.readbyqxmd.com/read/28811028/advanced-nanotechnologies-in-avian-influenza-current-status-and-future-trends-a-review
#3
REVIEW
Amitava Moulick, Lukas Richtera, Vedran Milosavljevic, Natalia Cernei, Yazan Haddad, Ondrej Zitka, Pavel Kopel, Zbynek Heger, Vojtech Adam
In the last decade, the control of avian influenza virus has experienced many difficulties, which have caused major global agricultural problems that have also led to public health consequences. Conventional biochemical methods are not sufficient to detect and control agricultural pathogens in the field due to the growing demand for food and subsidiary products; thus, studies aiming to develop potent alternatives to conventional biochemical methods are urgently needed. In this review, emerging detection systems, their applicability to diagnostics, and their therapeutic possibilities in view of nanotechnology are discussed...
August 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28810248/antibiotic-susceptibility-and-molecular-characterization-of-escherichia-coli-o157-isolates-from-urinary-tract-infections
#4
Zonglan Xie, Ziqiang Dong, Ping Zhu, Lusheng Zhang, Xiaobo Chen, Chuanjiang Dong
BACKGROUND: Escherichia coli O157 is an important food-borne pathogen that can cause diarrhoea, hemorrhagic colitis, and hemolytic uraemic syndrome. Enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and multilocus sequence typing (MLST) are good methods for molecular typing and the extensive use of antibiotics is a contributing factor to the increasing incidence of antimicrobial-resistant for these strains. OBJECTIVES: The aim of this study was to determine the prevalence, antimicrobial resistance, and genetic diversity of E...
August 16, 2017: Urologia Internationalis
https://www.readbyqxmd.com/read/28808161/listeria-monocytogenes-has-both-a-bd-type-and-an-aa3-type-terminal-oxidase-which-allow-growth-in-different-oxygen-levels-and-both-are-important-in-infection
#5
David Corbett, Marie Goldrick, Vitor E Fernandes, Kelly Davidge, Robert K Poole, Peter W Andrew, Jennifer Cavet, Ian S Roberts
Listeria monocytogenes is a food borne pathogen responsible for a number of life-threatening infections of humans. During an infection it invades epithelial cells before spreading from the intestine to the cells of the liver and spleen. This requires an ability to adapt to varying oxygen levels. Here we demonstrate that L. monocytogenes has two terminal oxidases, a cytochrome bd-type (CydAB) and a cytochrome aa3 -type menaquinol oxidase (QoxAB) and that both are used for respiration under different oxygen tensions...
August 14, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28807840/the-heterologous-expression-strategies-of-antimicrobial-peptides-in-microbial-systems
#6
REVIEW
Ting Deng, Haoran Ge, Huahua He, Yao Liu, Chao Zhai, Liang Feng, Li Yi
Antimicrobial peptides (AMPs) consist of molecules acting on the defense systems of numerous organisms toward tumor and multiple pathogens, such as bacteria, fungi, viruses, and parasites. Compared to traditional antibiotics, AMPs are more stable and have lower propensity for developing resistance through functioning in the innate immune system, thus having important applications in the fields of medicine, food and so on. However, despite of their high economic values, the low yield and the cumbersome extraction process in AMPs production are problems that limit their industrial application and scientific research...
August 11, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28807097/genotypic-characterization-of-methicillin-resistant-staphylococcus-aureus-isolated-from-pigs-and-retail-foods-in-china
#7
Wei Wang, Feng Liu, Zulqarnain Baloch, Cun Shan Zhang, Ke Ma, Zi Xin Peng, Shao Fei Yan, Yu Jie Hu, Xin Gan, Yin Ping Dong, Yao Bai, Feng Qin Li, Xiao Mein Yan, Ai Guo Ma, Jin Xu
OBJECTIVE: To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus (MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. METHODS: Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing (MLST), spa typing, multiple-locus variable number of tandem repeat analysis (MLVA), pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibility testing...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28805472/network-experiences-from-a-cross-sector-biosafety-level-3-laboratory-collaboration-a-swedish-forum-for-biopreparedness-diagnostics
#8
Johanna Thelaus, Anna Lindberg, Susanne Thisted Lambertz, Mona Byström, Mats Forsman, Hans Lindmark, Rickard Knutsson, Viveca Båverud, Andreas Bråve, Pontus Jureen, Annelie Lundin Zumpe, Öjar Melefors
The Swedish Forum for Biopreparedness Diagnostics (FBD) is a network that fosters collaboration among the 4 agencies with responsibility for the laboratory diagnostics of high-consequence pathogens, covering animal health and feed safety, food safety, public health and biodefense, and security. The aim of the network is to strengthen capabilities and capacities for diagnostics at the national biosafety level-3 (BSL-3) laboratories to improve Sweden's biopreparedness, in line with recommendations from the EU and WHO...
August 14, 2017: Health Security
https://www.readbyqxmd.com/read/28805457/selection-of-surrogate-bacteria-for-use-in-food-safety-challenge-studies-a-review
#9
Mengyi Hu, Joshua B Gurtler
Nonpathogenic surrogate bacteria are prevalently used in a variety of food challenge studies in place of foodborne pathogens such as Listeria monocytogenes, Salmonella, Escherichia coli O157:H7, and Clostridium botulinum because of safety and sanitary concerns. Surrogate bacteria should have growth characteristics and/or inactivation kinetics similar to those of target pathogens under given conditions in challenge studies. It is of great importance to carefully select and validate potential surrogate bacteria when verifying microbial inactivation processes...
August 14, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28804441/comparison-study-on-antioxidant-dna-damage-protective-and-antibacterial-activities-of-eugenol-and-isoeugenol-against-several-foodborne-pathogens
#10
Liang-Liang Zhang, Li-Fang Zhang, Jian-Guo Xu, Qing-Ping Hu
Eugenol and its isomer isoeugenol are both used as flavouring agents or food additives in food products, and have both some similar biological properties. However, the difference in biological activities between eugenol and isoeugenol is rarely studied. In this study, the profiles of antioxidant, DNA-protective effects and antibacterial activities of eugenol and isoeugenol against several common foodborne pathogens were investigated and compared under various experiment conditions. Results showed that eugenol and isoeugenol had strong antioxidant activity, the protective effect against DNA damage and antibacterial activity...
2017: Food & Nutrition Research
https://www.readbyqxmd.com/read/28803156/sequential-transition-of-the-injury-phenotype-temperature-dependent-survival-and-transcriptional-response-in-listeria-monocytogenes-following-lethal-h2o2-exposure
#11
Yoshitsugu Ochiai, Fumiya Yamada, Yuko Yoshikawa, Mariko Mochizuki, Takashi Takano, Ryo Hondo, Fukiko Ueda
The food-borne pathogen Listeria monocytogenes is present persistently in food processing environments, where this bacterium is exposed to various stress factors, including oxidative stress. This study aimed to elucidate the temperature-dependent response of L. monocytogenes to H2O2 exposure and the phenotypic changes in colony formation by H2O2-treated bacteria. Survival curves indicated an increase in the resistance to H2O2 in L. monocytogenes as the temperature decreased during the stress exposure procedure...
August 3, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28803008/two-step-large-volume-magnetic-separation-combined-with-pcr-assay-for-sensitive-detection-of-listeria-monocytogenes-in-pasteurized-milk
#12
Dan Luo, Xiaolin Huang, Yan Mao, Chaochao Chen, Fulai Li, Hengyi Xu, Yonghua Xiong
Immunomagnetic separation (IMS) is an effective tool for the preconcentration and purification of food-borne pathogens from complex food samples because of its high capture efficiency (CE). In conventional IMS, antibodies are usually conjugated on the surface of magnetic beads (MB); the random orientation and conformation changes of antibodies on the MB surface can decrease their bioactivity. Moreover, the Brownian motion of immobilized antibodies is weakened, thereby rendering their binding efficiency with bacteria lower than that of free antibodies...
August 9, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28803003/pathogenic-features-and-characteristics-of-food-borne-pathogens-biofilm-biomass-viability-and-matrix
#13
REVIEW
Shiqi Lin, Ling Yang, Chen Gu, Bing Li, Dingqiang Chen, Lin Li, Zhenbo Xu
Biofilm is a ubiquitous growth pattern of bacterial species survival but is notorious for its threat on public health and food contamination. Extensive studies of the biofilm structure, formation, quantification, quorum sensing system and underlying control strategies have been reported during the past decades. Insightful elucidation of the pathogenic features and characteristic of bacterial biofilm can facilitate in devising appropriate control strategies for biofilm eradication. Therefore, this review mainly summarized the pathogenic features of biofilms from food borne microorganisms, including the biomass (which could be quantified using crystal violet and fluorogenic dye Syto9 assays), viability (which could be determined by tetrazolium salts, fluorescein diacetate, resazurin staining and alamar blue assays) and matrix (which are commonly detected by dimethyl methylene blue and wheat germ agglutinin assays)...
August 9, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28802707/boosting-innate-immunity-to-sustainably-control-diseases-in-crops
#14
REVIEW
Valerie Nicaise
Viruses cause epidemics in all major crops, threatening global food security. The development of efficient and durable resistance able to withstand viral attacks represents a major challenge for agronomy, and relies greatly on the understanding of the molecular dialogue between viral pathogens and their hosts. Research over the last decades provided substantial advances in the field of plant-virus interactions. Remarkably, the advent of studies of plant innate immunity has recently offered new strategies exploitable in the field...
August 10, 2017: Current Opinion in Virology
https://www.readbyqxmd.com/read/28802266/functional-analysis-of-genes-encoded-by-the-locus-of-heat-resistance-lhr-in-escherichia-coli
#15
Ryan Mercer, Oanh Nguyen, Qixing Ou, Lynn McMullen, Michael G Gänzle
The locus of heat resistance (LHR) is a 15 - 19 kb genomic island conferring exceptional heat resistance to organisms in the family Enterobacteriaceae including pathogenic strains of Salmonella enterica and Escherichia coli The complement of LHR-encoded genes that is necessary for heat resistance and the stress- or growth-phase induced expression of LHR-encoded genes are unknown. This study determined the contribution of the 7 LHR-encoded genes yfdX1GI, yfdX2, hdeDGI, orf11, trxGI, kefB, and psiEGI by comparison of the heat resistance of E...
August 11, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28800831/characterization-of-enterococcus-durans-152-bacteriocins-and-their-inhibition-of-listeria-monocytogenes-in-ham
#16
Lihui Du, Fang Liu, Ping Zhao, Tong Zhao, Michael P Doyle
Listeria monocytogenes is a nonfastidious, widely occurring foodborne pathogen that is a major challenge to the food industry. Enterococcus durans 152, a confirmed L. monocytogenes-control microorganism, was isolated from floor drain samples from a food processing facility. In this study, the two bacteriocins produced by E. durans 152 were characterized and identified as Dur 152A (an enterocin L50A derivative with two amino acid substitutions of I→M) and enterocin L50B. The bacteriocins were then partially purified and evaluated for inhibitory activity to L...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800825/efficacy-evaluation-of-a-new-water-sanitizer-for-increasing-the-shelf-life-of-southern-australian-king-george-whiting-and-tasmanian-atlantic-salmon-fillets
#17
Manouchehr Khazandi, Permal Deo, Sergio Ferro, Henrietta Venter, Hongfei Pi, Simon Crabb, Tony Amorico, Abiodun D Ogunniyi, Darren J Trott
The bacterial species and specific spoilage organisms associated with the Southern Australian King George Whiting (KGW) and Tasmanian Atlantic Salmon (TAS), and the efficacy of a HOCl-containing water-based sanitization product (Electro-Chemically Activated Solution, by ECAS4) in extending the shelf life of KGW and TAS fillets were evaluated. Fillets were washed with an ECAS4 solution containing either 45 ppm or 150 ppm of free chlorine and bacterial species enumerated on selective and non-selective media, followed by identification of pure isolates by 16 S rRNA gene sequencing...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800824/assessing-the-antimicrobial-potential-of-aerosolised-electrochemically-activated-solutions-ecas-for-reducing-the-microbial-bio-burden-on-fresh-food-produce-held-under-cooled-or-cold-storage-conditions
#18
R M S Thorn, J Pendred, D M Reynolds
The main aim of this study was to assess the antimicrobial efficacy of electrochemically activated fog (ECAF) for reducing the microbial bio-burden on artificially inoculated fresh produce held under cooled (cucumber and vine tomatoes) or cold (rocket and broccoli) storage conditions. The ECAF treatment (1100 ± 5 mV ORP; 50 ± 5 mg L(-1) free chlorine; 2.7 ± 0.1 pH) resulted in a significant log reduction in the potential pathogen E. coli recovered from rocket (2.644 Log10 CFU g(-1)), broccoli (4...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800822/escherichia-coli-transfer-from-simulated-wildlife-feces-to-lettuce-during-foliar-irrigation-a-field-study-in-the-northeastern-united-states
#19
Daniel L Weller, Jasna Kovac, David J Kent, Sherry Roof, Jeffrey I Tokman, Erika Mudrak, Barbara Kowalcyk, David Oryang, Anna Aceituno, Martin Wiedmann
Wildlife intrusion has been associated with pathogen contamination of produce. However, few studies have examined pathogen transfer from wildlife feces to pre-harvest produce. This study was performed to calculate transfer coefficients for Escherichia coli from simulated wildlife feces to field-grown lettuce during irrigation. Rabbit feces inoculated with a 3-strain cocktail of non-pathogenic E. coli were placed in a lettuce field 2.5-72 h before irrigation. Following irrigation, the E. coli concentration on the lettuce was determined...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800816/an-investigation-of-vtx2-bacteriophage-transduction-to-different-escherichia-coli-patho-groups-in-food-matrices-and-nutrient-broth
#20
Sepo Nyambe, Catherine Burgess, Paul Whyte, Declan Bolton
This study investigated bacteriophage (phage) mediated transfer of the vtx2 gene from a donor Escherichia coli (C600φ3538(Δvtx2::cat)) to enteropathogenic (EPEC), enterotoxigenic (ETEC), enteroaggregative (EAEC), enteroinvasive (EIEC) and diffusely adherent (DAEC) E. coli strains in LB broth, milk, ground beef and lettuce. Two bacterial concentrations for both the E. coli donor and recipient strains, 3 and 5 log10 CFU/ml (LB broth and milk)/g (beef) or/cm(2) (lettuce), were used. When transductants were obtained, the location of insertion of the phage (insertion sites wrbA, yehA, sbcB, yecE and/or Z2577) in the E...
December 2017: Food Microbiology
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