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https://www.readbyqxmd.com/read/29653150/validation-of-reference-genes-for-quantitative-gene-expression-analysis-in-ralstonia-pseudosolanacearum-cqps-1-under-environment-stress
#1
Liang Yang, Juanni Chen, Ying Liu, Shuting Zhang, Shili Li, Wei Ding
Quantitative real-time reverse transcriptase PCR (qRT-PCR) has become the method choice for quantification of gene expression changes, however, the accuracy of the method depends on the stability of reference genes. Ralstonia pseudosolanacearum (R. pseudosolanacearum) is an important plant pathogen, infecting >450 plant species and causing bacterial wilt. In order to identify stable reference genes in R. pseudosolanacearum CQPS-1 under different environment stresses. We used five tools (△Ct method, GeNorm, NormFinder, BestKeeper, and RefFinder) to evaluate the stability of seven candidate reference genes including phosphoglycerate kinase (PGK), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 16S ribosomal RNA (16S), cell division protein ftsZ (ftsZ), DNA gyrase subunit A (gyrA), Ribosomal protein L13 (rplM), and phosphoserine aminotransferase (serC) under biotic (growth phases) and abiotic stress (temperature, hydroxycoumarins, nutrition)...
April 10, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29614440/effect-of-phosphate-nutrition-on-growth-physiology-and-phosphate-transporter-expression-of-cucumber-seedlings
#2
Zakira Naureen, Arjun Sham, Hibatullah Al Ashram, Syed A Gilani, Salma Al Gheilani, Fazal Mabood, Javid Hussain, Ahmed Al Harrasi, Synan F AbuQamar
Although abundantly present in soils, inorganic phosphate (Pi) acquisition by plants is highly dependent on the transmembrane phosphate transporter (PT) gene family. Cucumber (Cucumis sativus) requires a large amount of phosphorus (P). The purpose of this study was to isolate the CsPT2-1 from cucumber roots, and to determine the influence of Pi nutrition on cucumber growth, metabolism and transcript levels of CsPT2-1 in tissues. Full length CsPT2-1 was cloned and phylogenetically identified. In two greenhouse experiments, P-deficient seedlings provided with low or high P concentrations were sampled at 10 and 21 days post treatment, respectively...
March 26, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29610209/an-spx-rli1-module-regulates-leaf-inclination-in-response-to-phosphate-availability-in-rice
#3
Wenyuan Ruan, Meina Guo, Lei Xu, Xueqing Wang, Hongyu Zhao, Junmin Wang, Keke Yi
Leaf erectness is one of the key traits of plant architecture; in grains, plants with upright leaves can be planted close together, thus benefiting yield/unit area. Many factors, such as hormones, affect leaf inclination; however, how nutrition status, in particular phosphate (Pi) availability, affects leaf inclination remains largely unexplained. Here, we show that in rice (Oryza sativa), Pi-deficiency stress inhibits lamina joint cell elongation, thus restricting lamina joint size and inducing leaf erectness in rice...
April 2, 2018: Plant Cell
https://www.readbyqxmd.com/read/29571004/signal-transduction-in-artichoke-cynara-cardunculus-l-subsp-scolymus-l-hayek-callus-and-cell-suspension-cultures-under-nutritional-stress
#4
Vincenzo Lattanzio, Sofia Caretto, Vito Linsalata, Giovanni Colella, Giovanni Mita
Stimulated production of secondary phenolic metabolites and proline was studied by using cell cultures of artichoke [Cynara cardunculus L. subsp. scolymus (L.) Hayek] submitted to nutritional stress. Artichoke cell cultures accumulated phenolic secondary metabolites in a pattern similar to that seen in artichoke leaves and heads (capitula). This paper shows that both callus and cell suspension cultures under nutritional stress accumulated phenolic compounds and proline, at the same time their biomass production was negatively affected by nutrient deficiency...
March 16, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29533151/comparison-of-renal-function-and-other-predictors-in-lacto-ovo-vegetarians-and-omnivores-with-chronic-kidney-disease
#5
Chou-Yueh Chang, Horng-Rong Chang, Hsing-Chun Lin, Han-Hsin Chang
Objective Vegetarian diets have been shown to increase the risk of certain nutritional deficiencies, such as iron. As a number of patients with chronic kidney disease (CKD) in Taiwan are lacto-ovo vegetarians, the aim of this study was to investigate the effects of different proportions and sources of protein in lacto-ovo vegetarian and omnivorous diets, as well as the influence of adequate dietary protein intake, on renal function and nutritional status of Taiwanese patients with stage 3 to stage 5 CKD. Methods This is a cross-sectional study...
March 13, 2018: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/29515604/heterologous-expression-of-secreted-bacterial-bpp-and-hap-phytases-in-plants-stimulates-arabidopsis-thaliana-growth-on-phytate
#6
Lia R Valeeva, Chuluuntsetseg Nyamsuren, Margarita R Sharipova, Eugene V Shakirov
Phytases are specialized phosphatases capable of releasing inorganic phosphate from myo -inositol hexakisphosphate (phytate), which is highly abundant in many soils. As inorganic phosphorus reserves decrease over time in many agricultural soils, genetic manipulation of plants to enable secretion of potent phytases into the rhizosphere has been proposed as a promising approach to improve plant phosphorus nutrition. Several families of biotechnologically important phytases have been discovered and characterized, but little data are available on which phytase families can offer the most benefits toward improving plant phosphorus intake...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29450121/glyphosate-induced-toxicity-to-chickpea-plants-and-stress-alleviation-by-herbicide-tolerant-phosphate-solubilizing-burkholderia-cepacia-psbb1-carrying-multifarious-plant-growth-promoting-activities
#7
Mohammad Shahid, Mohd Saghir Khan
In this study, strain PSBB1 isolated from Vicia faba rhizosphere was identified as Burkholderia cepacia , by 16S rDNA sequence analysis and characterized. Strain PSBB1 tolerated glyphosate up to 3200 μg ml-1 and produced IAA (81.6 μg ml-1 ), ACC deaminase (69.3 mg-1  protein h-1 ), SA (39.3 μg ml-1 ) and 2,3-DHBA (26.6 μg ml-1 ), solubilized insoluble P (50.8 μg ml-1 ) and secreted 29.4 μg ml-1 exopolysaccharides, which decreased with increasing concentrations of glyphosate. Cell damage following glyphosate application was visible under SEM and CLSM...
February 2018: 3 Biotech
https://www.readbyqxmd.com/read/29421750/functionalized-biochar-derived-from-heavy-metal-rich-feedstock-phosphate-recovery-and-reusing-the-exhausted-biochar-as-an-enriched-soil-amendment
#8
Ahmed Mosa, Ayman El-Ghamry, Mona Tolba
This paper provides a circular win-win approach for recycling rhizofiltration biomass into multifunctional engineered biochar for various environmental applications (e.g. phosphate recovery) with a potential reuse of the exhausted biochar as an enriched soil amendment. Functionalized biochars were derived from the disposals of water hyacinth (Eichhornia crassipes) plants grown in synthetic contaminated water spiked with either Fe2+ (Fe-B), Mn2+ (Mn-B), Zn2+ (Zn-B) or Cu2+ (Cu-B) comparing with the original drainage water as a control treatment (O-B)...
February 1, 2018: Chemosphere
https://www.readbyqxmd.com/read/29304231/buffered-delivery-of-phosphate-to-arabidopsis-alters-responses-to-low-phosphate
#9
Meredith T Hanlon, Swayamjit Ray, Patompong Saengwilai, Dawn Luthe, Jonathan P Lynch, Kathleen M Brown
Arabidopsis has been reported to respond to phosphate (Pi) stress by arresting primary root growth and increasing lateral root branching. We developed a system to buffer Pi availability to Arabidopsis in gel media systems by charging activated aluminum oxide particles with low and sufficient concentrations of Pi, based on previous work in horticultural and sand culture systems. This system more closely mimics soil chemistry and results in different growth and transcriptional responses to Pi stress compared with plants grown in standard gel media...
January 3, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29258443/exogenous-trehalose-improves-growth-under-limiting-nitrogen-through-upregulation-of-nitrogen-metabolism
#10
Yingchao Lin, Jie Zhang, Weichang Gao, Yi Chen, Hongxun Li, David W Lawlor, Matthew J Paul, Wenjie Pan
BACKGROUND: The trehalose (Tre) pathway has strong effects on growth and development in plants through regulation of carbon metabolism. Altering either Tre or trehalose 6-phosphate (T6P) can improve growth and productivity of plants as observed under different water availability. As yet, there are no reports of the effects of modification of Tre orT6P on plant performance under limiting nutrition. RESULTS: Here we report that nitrogen (N) metabolism is positively affected by exogenous application of Tre in nitrogen-deficient growing conditions...
December 19, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29202269/opportunities-and-challenges-in-the-use-of-mineral-nutrition-for-minimizing-arsenic-toxicity-and-accumulation-in-rice-a-critical-review
#11
REVIEW
Saifullah, Saad Dahlawi, Asif Naeem, Muhammad Iqbal, Muhammad Ansar Farooq, Sadia Bibi, Zed Rengel
Growing rice on arsenic (As)-contaminated soil or irrigating with As-contaminated water leads to significant accumulation of As in grains. Moreover, rice accumulates more As into grains than other cereal crops. Thus, rice consumption has been identified as a major route of human exposure to As in many countries. Inorganic As species are carcinogenic and could pose a considerable health risk to humans even at low dietary concentration. Genotypic variation and concentration of nutrients such as iron, manganese, phosphate, sulfur and silicon are the two main factors that affect As accumulation in rice grains...
March 2018: Chemosphere
https://www.readbyqxmd.com/read/29200724/bioassay-guided-isolation-of-neuroprotective-fatty-acids-from-nigella-sativa-against-1-methyl-4-phenylpyridinium-induced-neurotoxicity
#12
Leila Hosseinzadeh, Hoda Monaghash, Farahnaz Ahmadi, Nastaran Ghiasvand, Yalda Shokoohinia
Objective: Parkinson's disease, a slowly progressive neurological disease, is associated with degeneration of the basal ganglia of the brain and a deficiency of the neurotransmitter dopamine. The main aspects of researches are the protection of normal neurons against degeneration. Fatty acids (FAs), the key structural elements of dietary lipids, are carboxylic straight chains and notable parameters in nutritional and industrial usefulness of a plant. Materials and Methods: Black cumin, a popular anti-inflammatory and antioxidant food seasoning, contains nonpolar constituents such as FAs which were extracted using hexane...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29193665/provitamin-a-biofortification-of-cassava-enhances-shelf-life-but-reduces-dry-matter-content-of-storage-roots-due-to-altered-carbon-partitioning-into-starch
#13
Getu Beyene, Felix R Solomon, Raj D Chauhan, Eliana Gaitán-Solis, Narayanan Narayanan, Jackson Gehan, Dimuth Siritunga, Robyn L Stevens, John Jifon, Joyce Van Eck, Edward Linsler, Malia Gehan, Muhammad Ilyas, Martin Fregene, Richard T Sayre, Paul Anderson, Nigel J Taylor, Edgar B Cahoon
Storage roots of cassava (Manihot esculenta Crantz), a major subsistence crop of sub-Saharan Africa, are calorie rich but deficient in essential micronutrients, including provitamin A β-carotene. In this study, β-carotene concentrations in cassava storage roots were enhanced by co-expression of transgenes for deoxy-d-xylulose-5-phosphate synthase (DXS) and bacterial phytoene synthase (crtB), mediated by the patatin-type 1 promoter. Storage roots harvested from field-grown plants accumulated carotenoids to ≤50 μg/g DW, 15- to 20-fold increases relative to roots from nontransgenic plants...
November 28, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29167981/transcriptional-induction-of-two-phosphate-transporter-1-genes-and-enhanced-root-branching-in-grape-plants-inoculated-with-funneliformis-mosseae
#14
Laure Valat, Laurence Deglène-Benbrahim, Melha Kendel, Réjane Hussenet, Christine Le Jeune, Paul Schellenbaum, Pascale Maillot
We investigated the effects of the arbuscular mycorrhizal fungus (AMF) Funneliformis mosseae on the growth and root architecture of plantlets of the grape rootstock 41B MGt under hydroponic conditions, and analyzed the concomitant expression of putative mycorrhizal-specific phosphate transporter 1 (PHT1) genes. In vitro propagated plantlets were acclimatized to ex vitro culture before AMF inoculation and grown under low phosphate (Pi) nutrition conditions during 6 weeks. Grape roots could be efficiently colonized by F...
February 2018: Mycorrhiza
https://www.readbyqxmd.com/read/29165807/arabidopsis-thaliana-plants-challenged-with-uranium-reveal-new-insights-into-iron-and-phosphate-homeostasis
#15
Serge Berthet, Florent Villiers, Claude Alban, Nelson B C Serre, Jacqueline Martin-Laffon, Sylvie Figuet, Anne-Marie Boisson, Richard Bligny, Marcel Kuntz, Giovanni Finazzi, Stéphane Ravanel, Jacques Bourguignon
Uranium (U) is a naturally occurring radionuclide that is toxic to plants. It is known to interfere with phosphate nutrition and to modify the expression of iron (Fe)-responsive genes. The transporters involved in the uptake of U from the environment are unknown. Here, we addressed whether IRT1, a high-affinity Fe2+ transporter, could contribute to U uptake in Arabidopsis thaliana. An irt1 null mutant was grown hydroponically in different conditions of Fe bioavailability and phosphate supply, and challenged with uranyl...
January 2018: New Phytologist
https://www.readbyqxmd.com/read/29148171/identification-and-expression-profiling-of-pht1-phosphate-transporters-in-wheat-in-controlled-environments-and-in-the-field
#16
A Grün, P Buchner, M R Broadley, M J Hawkesford
Phosphorus (P) is an important macronutrient with critical functions in plants. Phosphate (Pi) transporters, which mediate Pi acquisition and Pi translocation within the plant, are key factors in Pi deficiency responses. However, their relevance for adaptation to long-term Pi limitation under agronomic conditions, particularly in wheat, remains unknown. Here, we describe the identification of the complete Pi transporter gene family (Pht1) in wheat (Triticum aestivum). Gene expression profiles were compared for hydroponic and field-grown plant tissues of wheat at multiple development stages...
March 2018: Plant Biology
https://www.readbyqxmd.com/read/29144540/biosynthetic-energy-cost-of-potentially-highly-expressed-proteins-vary-with-niche-in-selected-actinobacteria
#17
Indrani Sarkar, Louis S Tisa, Maher Gtari, Arnab Sen
Amino acid and protein biosynthesis requires a number of high energy phosphate bonds and includes a dual energy cost for the synthesis of chemical intermediates during the fueling reactions and the conversion of precursor molecules to final products. One popular hypothesis is that the proteins encoded by putative highly expressed genes (hence called PHXPs) generally utilize low energy consuming amino acids to reduce the biosynthetic cost of the essential proteins. In our study, we found that this idea was not supported in the case of actinobacteria...
November 16, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/29125620/metabolite-profiling-of-peppers-of-various-colors-reveals-relationships-between-tocopherol-carotenoid-and-phytosterol-content
#18
Tae Jin Kim, Jaehyuk Choi, Kil Won Kim, Soon Kil Ahn, Sun-Hwa Ha, Yongsoo Choi, Nam Il Park, Jae Kwang Kim
Peppers are widely consumed in Korea; the varietal development of peppers with increased content of beneficial plant metabolites is, therefore, of considerable interest. This requires a comprehensive understanding of the metabolic profile of pepper plants and the factors affecting this profile. To this end, we determined the content of various metabolites, such as hydrophilic and lipophilic compounds, phenolic acids, carotenoids, and capsaicinoids in peppers of various colors (green, red, pale green, and violet peppers) and in a high-pungency (green) pepper...
December 2017: Journal of Food Science
https://www.readbyqxmd.com/read/29110555/oxidative-stress-or-redox-signalling-new-insights-into-the-effects-of-a-proprietary-multifunctional-botanical-dietary-supplement
#19
Boris V Nemzer, Christoph Centner, Denise Zdzieblik, Bruno Fink, John M Hunter, Daniel König
Recent interest has focused on maintenance of healthy levels of redox signalling and the related oxidants; these parameters are crucial for providing us with concrete nutritional targets that may help us to better understand and maintain "optimal health". Following the above hypothesis, we performed a pilot double-blind, crossover, placebo-controlled, single dose study to measure the dose-dependent effects of a proprietary plant-based dietary supplement labelled here as S7 (SPECTRA7), related to how it affected the cellular metabolic index (CMI) in healthy human participants (n = 8)...
November 7, 2017: Free Radical Research
https://www.readbyqxmd.com/read/29109915/mechanisms-of-oat-avena-sativa-l-acclimation-to-phosphate-deficiency
#20
Ewa Żebrowska, Marta Milewska, Iwona Ciereszko
Background: Deficiency of available forms of phosphorus is common in most soils and causes reduction of crop plants growth and yield. Recently, model plants responses to phosphate (Pi) deficiency have been intensively studied. However, acclimation mechanisms of cereals like oat ( Avena sativa L.), to low Pi stress remains not fully understood. Oat plants have been usually cultured on poor soils, with a low nutrient content, but their responses to such conditions are not well known, therefore the main goal of the study was to investigate the mechanisms that enable oat plants to grow under low Pi conditions...
2017: PeerJ
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