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https://www.readbyqxmd.com/read/28319800/inhibition-of-salmonella-typhimurium-on-radish-sprouts-using-nitrogen-cold-plasma
#1
Yeong Ji Oh, A Young Song, Sea C Min
This study investigated the effects of cold plasma treatment (CPT) on the inhibition of Salmonella typhimurium on radish sprouts and the quality attributes of the sprouts. Radish sprouts were treated with nitrogen (N2)-cold plasma at 900W and 667Pa for 0, 2, 5, 10, and 20min using a microwave-powered CPT system. The sensory attributes of the radish sprouts, appearance and odor, were evaluated before and after the treatment. The effects of N2-CPT for 10min on microbial growth and the quality attributes of the radish sprouts were evaluated during storage for 12days at 4 and 10°C...
March 10, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28317842/bde-99-impairs-differentiation-of-human-and-mouse-npcs-into-the-oligodendroglial-lineage-by-species-specific-modes-of-action
#2
Katharina Dach, Farina Bendt, Ulrike Huebenthal, Susanne Giersiefer, Pamela J Lein, Heike Heuer, Ellen Fritsche
Polybrominated diphenyl ethers (PBDEs) are bioaccumulating flame retardants causing developmental neurotoxicity (DNT) in humans and rodents. Their DNT effects are suspected to involve thyroid hormone (TH) signaling disruption. Here, we tested the hypothesis whether disturbance of neural progenitor cell (NPC) differentiation into the oligodendrocyte lineage (O4(+) cells) by BDE-99 involves disruption of TH action in human and mouse (h,m)NPCs. Therefore, we quantified differentiation of NPCs into O4(+) cells and measured their maturation via expression of myelin-associated genes (hMBP, mMog) in presence and absence of TH and/or BDE-99...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28317754/first-report-on-isolation-of-2-3-4-trihydroxy-5-methylacetophenone-from-palmyra-palm-borassus-flabellifer-linn-syrup-its-antioxidant-and-antimicrobial-properties
#3
M V Reshma, Jubi Jacob, V L Syamnath, V P Habeeba, B S Dileep Kumar, Ravi S Lankalapalli
The first report on isolation and characterization of 2,3,4-trihydroxy-5-methylacetophenone (1), nicotinamide (2), and uracil (3) from palmyra palm syrup is described. Total phenolic content (TPC) and Total flavonoid content (TFC) of palm syrup were 244.70±5.77(mggallic acid/kg of syrup) and 658.45±27.86(mg quercetin/kg of syrup), respectively. Compound 1 exhibited DPPH radical scavenging activity with an IC50 value of 20.02±0.14μM which was better than ascorbic acid (IC50=22.59±0.30μM). Compound 1 also showed broad spectrum antibacterial activity against Escherichia coli, Mycobacterium smegmatis, Staphylococcus aureus and Staphylococcus simulans...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28317739/physicochemical-changes-of-phulae-pineapple-fruit-treated-with-short-term-anoxia-during-ambient-storage
#4
Chairat Techavuthiporn, Panida Boonyaritthongchai, Suriyan Supabvanich
The effects of short-term anoxia exposure for 16h on physicochemical changes of 'Phulae' pineapple fruit stored at ambient temperature (25±2°C) were investigated. The respiratory rate of the fruit was induced by the anoxia treatment. However, it retarded the increase in moisture loss and maintained both flesh and pulp colour by inhibiting polyphenol oxidase (PPO) activity of the both tissues. The anoxia exposure delayed the increase in total sugar content and enhanced total ascorbic acid content during storage...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28317715/evaluation-of-penicillium-expansum-for-growth-patulin-accumulation-nonvolatile-compounds-and-volatile-profile-in-kiwi-juices-of-different-cultivars
#5
Yuan Wang, Tingting Shan, Yahong Yuan, Zhiwei Zhang, Chunfeng Guo, Tianli Yue
Patulin (PAT) contamination and changes of nonvolatile and volatile compounds caused by Penicillium expansum in fresh juices of 8 kiwi cultivars were investigated. Growth and PAT production of P. expansum were greater at 25°C than at 4°C. P. expansum grew in kiwi juices under pH ranging from 2.75 to 3.27 and produced 45.10-268.88μg/mL of PAT at 25°C. Decreases occurred in malic acid and soluble protein; while consumption and production simultaneously happened for reducing sugar, total soluble solid, titratable acid, citric acid, ascorbic acid and total phenolics...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28317241/organogels-from-double-chained-vitamin-c-amphiphilic-derivatives
#6
Pierandrea Lo Nostro, Elia Tempestini, Martina Bucci, Vincenzo Mastromartino, Marianna Gori, Damiano Tanini, Moira Ambrosi, Emiliano Fratini, Antonella Capperucci
We report on the synthesis and physico-chemical characterization of double chained amphiphilic compounds obtained from vitamin C: Di-alkanoyl-5,6-O-ascorbic acid esters, Di-ASCn, with n = 8, 10 and 12. The acetyl-5-dodecanoyl-6-ascorbic acid ester was synthesized and investigated for comparison. These products are quite insoluble in water and in polar solvents, while they form homogeneous dispersions in cyclohexane. By cooling, these dispersions turn into a gel-like phase. DSC, FTIR, and SAXS/WAXS experiments were carried out to investigate the properties of the pure solids and of their liquid dispersions...
March 20, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28315301/metabolites-of-2-3-diketogulonate-delay-peroxidase-action-and-induce-non-enzymic-h2o2-generation-potential-roles-in-the-plant-cell-wall
#7
Anna Kärkönen, Rebecca A Dewhirst, C Logan Mackay, Stephen C Fry
A proportion of the plant's l-ascorbate (vitamin C) occurs in the apoplast, where it and its metabolites may act as pro-oxidants and anti-oxidants. One ascorbate metabolite is 2,3-diketogulonate (DKG), preparations of which can non-enzymically generate H2O2 and delay peroxidase action on aromatic substrates. As DKG itself generates several by-products, we characterised these and their ability to generate H2O2 and delay peroxidase action. DKG preparations rapidly produced a by-product, compound (1), with λmax 271 and 251 nm at neutral and acidic pH respectively...
March 14, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28306530/ascorbic-acid-does-not-modulate-potassium-currents-in-cultured-human-lymphocytes
#8
Saravanakumar Snekalatha, Praghalathan Kanthakumar
BACKGROUND: Ascorbic acid (AA) is known to modulate lymphocyte function, but the mechanism of action is not clearly understood. As voltage-gated potassium currents play an important role in lymphocyte function, the effect of AA on voltage-gated potassium currents was studied. METHODS: Peripheral blood mononuclear cells were cultured in the presence of increasing concentrations of AA (0, 0.125, 0.25, 0.5, and 1 mM). Potassium currents in resting lymphocytes were studied by whole cell patch clamp technique using a depolarizing protocol...
March 17, 2017: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/28303328/effects-of-palmitoyl-kvk-l-ascorbic-acid-on-skin-wrinkles-and-pigmentation
#9
Hyeong Mi Kim, Hyo Sun An, Jung-Soo Bae, Jung Yun Kim, Chi Ho Choi, Ju Yeon Kim, Joo Hyuck Lim, Joon-Hun Choi, Hyunnam Song, Sung Ho Moon, Young Jun Park, Shin-Jae Chang, Sun Young Choi
Wrinkle formation and abnormal pigmentation are major clinical alterations associated with skin aging. As the aim of our study was to investigate the effects of palmitoyl-KVK-L-ascorbic acid on skin aging, the anti-wrinkle and depigmentation effects of palmitoyl-KVK-L-ascorbic acid were evaluated by measuring collagen expression in dermal fibroblast cells and inhibition of melanogenesis in B16F1 cells, respectively. The anti-aging effect of palmitoyl-KVK-L-ascorbic acid cream was also evaluated against a placebo cream in a clinical trial...
March 16, 2017: Archives of Dermatological Research
https://www.readbyqxmd.com/read/28299689/extended-gate-field-effect-transistor-biosensors-for-point-of-care-testing-of-uric-acid
#10
Weihua Guan, Mark A Reed
An enzyme-free redox potential sensor using off-chip extended-gate field effect transistor (EGFET) with a ferrocenyl-alkanethiol modified gold electrode has been used to quantify uric acid concentration in human serum and urine. Hexacyanoferrate (II) and (III) ions are used as redox reagent. The potentiometric sensor measures the interface potential on the ferrocene immobilized gold electrode, which is modulated by the redox reaction between uric acid and hexacyanoferrate ions. The device shows a near Nernstian response to uric acid and is highly specific to uric acid in human serum and urine...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28298689/postharvest-application-of-brassica-meal-derived-allyl-isothiocyanate-to-kiwifruit-effect-on-fruit-quality-nutraceutical-parameters-and-physiological-response
#11
Luisa Ugolini, Laura Righetti, Katya Carbone, Roberta Paris, Lorena Malaguti, Alessandra Di Francesco, Laura Micheli, Mariano Paliotta, Marta Mari, Luca Lazzeri
The use of natural compounds to preserve fruit quality and develop high value functional products deserves attention especially in the growing industry of processing and packaging ready-to-eat fresh-cut fruit. In this work, potential mechanisms underlying the effects of postharvest biofumigation with brassica meal-derived allyl-isothiocyanate on the physiological responses and quality of 'Hayward' kiwifruits were studied. Fruits were treated with 0.15 mg L(-1) of allyl-isothiocyanate vapours for 5 h and then stored in controlled atmosphere (2% O2, 4...
March 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28293290/nitrogen-starvation-induced-cellular-crosstalk-of-ros-scavenging-antioxidants-and-phytohormone-enhanced-the-biofuel-potential-of-green-microalga-acutodesmus-dimorphus
#12
Kaumeel Chokshi, Imran Pancha, Arup Ghosh, Sandhya Mishra
BACKGROUND: Microalgae accumulate a considerable amount of lipids and carbohydrate under nutrient-deficient conditions, which makes them one of the promising sustainable resources for biofuel production. In the present study, to obtain the biomass with higher lipid and carbohydrate contents, we implemented a short-term nitrogen starvation of 1, 2, and 3 days in a green microalga Acutodesmus dimorphus. Few recent reports suggest that oxidative stress-tolerant microalgae are highly efficient for biofuel production...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28292703/seed-mediated-grown-silver-nanoparticles-as-a-colorimetric-sensor-for-detection-of-ascorbic-acid
#13
Simindokht Rostami, Ali Mehdinia, Ali Jabbari
A simple and sensitive approach was demonstrated for detection of ascorbic acid (AA) based on seed-mediated growth of silver nanoparticles (Ag NPs). According to the seeding strategy, silver ions existing in the growth solution were reduced to silver atoms on the surface of silver seeds via redox reaction between silver ions and AA. This process -led to appear an absorption band in near 420nm owing to the localized surface plasmon resonance peak of the generated Ag NPs. This change in absorption spectra of Ag NPs caused a change in color of the mixture from colorless to yellow...
March 7, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28291806/effects-of-silicon-on-oryza-sativa-l-seedling-roots-under-simulated-acid-rain-stress
#14
Shuming Ju, Ningning Yin, Liping Wang, Cuiying Zhang, Yukun Wang
Silicon (Si) has an important function in reducing the damage of environmental stress on plants. Acid rain is a serious abiotic stress factor, and Si can alleviate the stress induced by acid rain on plants. Based on these assumptions, we investigated the effects of silicon on the growth, root phenotype, mineral element contents, hydrogen peroxide (H2O2) and antioxidative enzymes of rice (Oryza sativa L.) seedling roots under simulated acid rain (SAR) stress. The results showed that the combined or single effects of Si and/or SAR on rice roots depend on the concentration of Si and the pH of the SAR...
2017: PloS One
https://www.readbyqxmd.com/read/28291348/isolation-of-native-proanthocyanidins-from-grapevine-vitis-vinifera-and-other-fruits-in-aqueous-buffer
#15
Jean-Marc Brillouet, Hélène Fulcrand, Stéphanie Carrillo, Laurent Rouméas, Charles Romieu
Condensed tannins (also called proanthocyanidins) present in strategic tissues of fruits (outer pericarp and vascular bundles) were known as short polymers of flavan-3-ols. A pretreatment of the plant material (fruits from the grapevine, persimmon) with buffered ascorbic acid and Triton X-100 followed by acetone extraction provided native white fully depolymerizable tannins. Tannins are most of time extracted with aqueous solvents and further purified, although artifactual oxidations occur altering their physicochemical characteristics...
March 14, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28291237/comparison-of-two-intensive-bowel-cleansing-regimens-in-patients-with-previous-poor-bowel-preparation-a-randomized-controlled-study
#16
Antonio Z Gimeno-García, Goretti Hernandez, Ana Aldea, David Nicolás-Pérez, Alejandro Jiménez, Marta Carrillo, Vanesa Felipe, Onofre Alarcón-Fernández, Manuel Hernandez-Guerra, Rafael Romero, Inmaculada Alonso, Yanira Gonzalez, Zaida Adrian, Miguel Moreno, Laura Ramos, Enrique Quintero
OBJECTIVES: Inadequate bowel cleansing is a major burden for endoscopy units. The aim of this study was to compare two intensive bowel cleansing regimens in patients with previous colonoscopy with inadequate bowel preparation. METHODS: Patients with inadequate cleansing at index colonoscopy were randomized to 4-L split-dose polyethylene-glycol (PEG) regimen vs. 2-L split-dose PEG plus ascorbic acid (PEG+Asc) regimen. All individuals underwent a 3-day low-residue diet and received 10 mg of bisacodyl, the day before colonoscopy...
March 14, 2017: American Journal of Gastroenterology
https://www.readbyqxmd.com/read/28290328/betaxanthins-and-antioxidant-capacity-in-stenocereus-pruinosus-stability-and-use-in-food
#17
Juan A Rodríguez-Sánchez, María T Cruz Y Victoria, Blanca E Barragán-Huerta
Betalains are important pigments for the food, pharmaceutical, and cosmetics industry. In the yellow Stenocereus pruinosus fruits (pitayas), total betalain concentration, Folin-Ciocalteu reduction capacity, and antiradical capacity per dry weight were 2345.9μgg(-1), 7.3mg gallic acid equivalentsg(-1), and 48.8μmol Trolox equivalentg(-1), respectively. The stability of betaxanthins, which represent 89% of total betalains in yellow pitayas, was evaluated over a range of pH, temperature, as well as in the presence of food additives...
January 2017: Food Research International
https://www.readbyqxmd.com/read/28288447/chemical-redox-modulated-fluorescence-of-nitrogen-doped-graphene-quantum-dots-for-probing-the-activity-of-alkaline-phosphatase
#18
JingJing Liu, Duosi Tang, Zhitao Chen, Xiaomei Yan, Zhou Zhong, Longtian Kang, Jiannian Yao
Alkaline phosphatase (ALP) as an essential enzyme plays an important role in clinical diagnoses and biomedical researches. Hence, the development of convenient and sensitivity assay for monitoring ALP is extremely important. In this work, on the basis of chemical redox strategy to modulate the fluorescence of nitrogen-doped graphene quantum dots (NGQDs), a novel label-free fluorescent sensing system for the detection of alkaline phosphatase (ALP) activity has been developed. The fluorescence of NGQDs is firstly quenched by ultrathin cobalt oxyhydroxide (CoOOH) nanosheets, and then restored by ascorbic acid (AA), which can reduce CoOOH to Co(2+), thus the ALP can be monitored based on the enzymatic hydrolysis of L-ascorbic acid-2-phosphate (AAP) by ALP to generate AA...
March 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28285367/comparison-of-protective-effect-of-ascorbic-acid-on-redox-and-endocannabinoid-systems-interactions-in-in-vitro-cultured-human-skin-fibroblasts-exposed-to-uv-radiation-and-hydrogen-peroxide
#19
Agnieszka Gęgotek, Katarzyna Bielawska, Michał Biernacki, Ilona Zaręba, Arkadiusz Surażyński, Elżbieta Skrzydlewska
The mechanisms of biological activity of commonly used natural compounds are constantly examined. Therefore, the aim of this study was to compare ascorbic acid efficacy in counteracting the consequences of UV and hydrogen peroxide treatment on lipid mediators and their regulative action on antioxidant abilities. Skin fibroblasts exposed to UVA and UVB irradiation, treated with hydrogen peroxide and ascorbic acid. The redox system was estimated through reactive oxygen species (ROS) generation (electron spin resonance spectrometer) and antioxidants level/activity (HPLC/spectrometry) which activity was evaluated by the level of phospholipid metabolites: 4-hydroxynonenal, malondialdehyde, 8-isoprostanes and endocannabinoids (GC/LC-MS) in the human skin fibroblasts...
March 11, 2017: Archives of Dermatological Research
https://www.readbyqxmd.com/read/28281746/a-near-infrared-graphene-quantum-dots-based-two-photon-nanoprobe-for-direct-bioimaging-of-endogenous-ascorbic-acid-in-living-cells
#20
Lili Feng, Yong-Xiang Wu, Dai-Liang Zhang, Xiaoxiao Hu, Jing Zhang, Peng Wang, Zhi-Ling Song, Xiao-Bing Zhang, Weihong Tan
Ascorbic acid (AA), as one of the most important vitamins, participates in various physiological reactions in human body and is implicated with many diseases. Therefore, the development of effective methods for monitoring AA level in living systems is of great significance. Up to date, various technologies have been developed for the detection of AA. However, few methods can realize the direct detection of endogenous AA in living cells. In this work, we for the first time reported that near infrared NIR graphene quantum dots (GQD) possessed good two-photon fluorescence properties with a NIR emission at 660 nm upon exciting with 810 nm femtosecond pulses, and a two-photon (TP) excitation action cross-section (δΦ) of 25...
March 10, 2017: Analytical Chemistry
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