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https://www.readbyqxmd.com/read/28636436/signal-on-fluorescent-sensor-based-on-n-cqds-for-the-detection-of-glutathione-in-human-serum-and-pharmaceutic-preparation
#1
Chengfei Zhao, Kangying Wang, Qihong Cai, Haijian Tu, Linghong Pan, Liumin Yu
Nitrogen-doped carbon quantum dots (N-CQDs) with citric acid and ethylenediamine as raw materials were synthesized by an efficient one-step strategy. The N-CQDs showed a special property that the fluorescence was quenched by Fe(3+). The quenched fluorescence of N-CQDs could be recovered by glutathione (GSH). Therefore, a "signal-on" fluorescent sensor was developed to detect GSH. The fluorescent sensor could favorably avoid the interference of ascorbic acid, dopamine, glucose, oxidized glutathione and other amino acids in the detecting process of GSH...
June 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28636279/effects-of-in-ovo-injection-of-chrysin-quercetin-and-ascorbic-acid-on-hatchability-somatic-attributes-hepatic-oxidative-status-and-early-post-hatch-performance-of-broiler-chicks
#2
F Khaligh, A Hassanabadi, H Nassiri-Moghaddam, A Golian, G-A Kalidari
An experiment was conducted to evaluate the effects of in ovo injection of chrysin, quercetin and ascorbic acid on hatchability, somatic attributes, hepatic antioxidant status and early post-hatch growth performance of broiler chicks. Four hundred and eighty embryonated broiler breeder eggs containing live 18-day-old embryos were divided into six groups of 80 eggs each. One group remained intact and served as a control group (i), whereas the other five groups were injected with the prepared injection solutions as follows: (ii) 0...
June 21, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28635261/novel-protein-based-solid-biosensor-for-determining-pro-oxidant-activity-of-phenolic-compounds
#3
Esin Akyüz, Kevser Sözgen Başkan, Esma Tütem, Reşat Apak
To develop a protein‒based biosensor measuring the pro-oxidant activities of phenolic compounds, egg white proteins were precipitated with calcium chloride to obtain an insoluble calcium proteinate complex. This biosensor was used for the determination of Cu(II)‒induced pro-oxidant activity of antioxidants such as gallic acid, catechin, epicatechin, quercetin, chlorogenic acid and myricetin, and ascorbic acid. This assay involved the reduction of Cu(II) ions to Cu(I) by antioxidant compounds (simultaneously giving rise to reactive oxygen species) and binding of the formed Cu(I) to the solid biosensor...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28634178/ascorbate-protects-the-di-heme-enzyme-maug-against-self-inflicted-oxidative-damage-by-an-unusual-antioxidant-mechanism
#4
Zhongxin Ma, Victor L Davidson
Ascorbate protects MauG from self-inactivation that occurs during the autoreduction of the reactive bis -Fe(IV) state of its diheme cofactor. The mechanism of protection does not involve direct reaction with reactive oxygen species in solution. Instead it binds to MauG and mitigates oxidative damage that occurs via internal transfer of electrons from amino acid residues within the protein to the high-valent hemes. The presence of ascorbate does not inhibit the natural catalytic reaction of MauG which catalyzes oxidative posttranslational modifications of a substrate protein that binds to the surface of MauG, and is oxidized by the high-valent hemes via long range electron transfer...
June 20, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28633386/oral-administration-of-tea-saponins-to-relive-oxidative-stress-and-immune-suppression-in-chickens
#5
X Chi, S Bi, W Xu, Y Zhang, S Liang, S Hu
The present study was designed to evaluate the effects of tea saponins on oxidative stress induced by cyclophosphamide in chickens. One hundred twenty chickens were randomly divided into 5 groups. Groups 3 to 4 received intramuscular injection of cyclophosphamide to induce oxidative stress and immunosuppression. After that, groups 2 and 4 were orally administered tea saponins in drinking water for 7 d. Then, groups 1 to 4 were immunized with a live, bivalent vaccine of Newcastle disease virus and infectious bronchitis virus...
June 15, 2017: Poultry Science
https://www.readbyqxmd.com/read/28631521/on-the-die-compaction-of-powders-used-in-pharmaceutics
#6
G Aryanpour, M Farzaneh
Die compaction is widely used in the compaction of pharmaceutical powders (tableting). It is well known that the powder densification is a result of particle rearrangement and particle deformation. The former is considered to be the governing mechanism of densification in an initial stage of compaction and the latter is regarded as the governing mechanism in the compaction at the higher pressure range. As a more realistic assumption, one can consider that a simultaneous performance of both the rearrangement and deformation mechanisms takes place from the beginning of compaction...
June 20, 2017: Pharmaceutical Development and Technology
https://www.readbyqxmd.com/read/28629017/design-of-a-new-nanocomposite-between-bismuth-nanoparticles-and-graphene-oxide-for-development-of-electrochemical-sensors
#7
Eduardo H Bindewald, Arthur F Schibelbain, Maurício A P Papi, Eduardo G C Neiva, Aldo J G Zarbin, Márcio F Bergamini, Luiz H Marcolino-Júnior
This study describes a new route for preparation of a nanocomposite between graphene oxide (GO) and bismuth nanoparticles (BiNPs) and its evaluation as modifier electrode for development of electrochemical sensors. BiNPs were synthesized under ultrasound conditions using Bi(NO3)3 as metal precursor and ascorbic acid (AA) as reducing agent/passivating. Some experimental parameters of BiNPs synthesis such as Bi(3+):AA molar ratio and reaction time were conducted aiming the best voltammetric performance of the sensor...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28627252/phytochemical-and-antioxidant-properties-of-some-cassia-species
#8
Firdose R Kolar, Chaya L Gogi, Mairunisabegum M Khudavand, Meera S Choudhari, Sindhu B Patil
In this study, the antioxidant activity of aqueous and ethanol extracts of four plants from the genus Cassia were evaluated by various antioxidant assays, including ferric reducing antioxidant power (FRAP), DPPH free radical scavenging, metal chelating activity, phosphomolybdenum reducing power, hydrogen peroxide radical scavenging, hydroxyl radical scavenging, deoxyribose degradation and β-carotene bleaching assay. The various antioxidant activities were compared to standard antioxidant such as ascorbic acid...
June 18, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28624857/bioaccumulation-of-heavy-metals-and-ecophysiological-responses-to-heavy-metal-stress-in-selected-populations-of-vaccinium-myrtillus-l-and-vaccinium-vitis-idaea-l
#9
Marta Kandziora-Ciupa, Aleksandra Nadgórska-Socha, Gabriela Barczyk, Ryszard Ciepał
The aim of this study was to determine the concentrations of heavy metals (Cd, Pb, Zn, Fe, and Mn) in soil, and their bioavailability and bioaccumulation in Vaccinium myrtillus L. and Vaccinium vitis-idaea L. organs. Analysis also concerned the physiological responses of these plants from three polluted sites (immediate vicinity of a zinc smelter in Miasteczko Śląskie, ArcelorMittal Poland S.A. iron smelter in Dąbrowa Górnicza-Łosień, and Jaworzno III power plant in Jaworzno) and one pseudo-control site (Pazurek nature reserve in Jaroszowiec Olkuski)...
June 17, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28624590/maleic-acid-assisted-improvement-of-metal-chelation-and-antioxidant-metabolism-confers-chromium-tolerance-in-brassica-juncea-l
#10
Jubayer Al Mahmud, Mirza Hasanuzzaman, Kamrun Nahar, Anisur Rahman, Md Shahadat Hossain, Masayuki Fujita
Chromium (Cr) is a highly toxic environmental pollutant that negatively affects plant growth and development. Thus, remediating Cr from soil or increasing plant tolerance against Cr stress is urgent. Organic acids are recognized as agents of phytoremediation and as exogenous protectants, but using maleic acid (MA) to attain these results has not yet been studied. Therefore, our study investigated the effects of MA on Cr uptake and mitigation of Cr toxicity. We treated 8-d-old Indian mustard (Brassica juncea L...
June 15, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28622916/osteogenic-potential-of-platelet-rich-plasma-in-dental-stem-cell-cultures
#11
L Otero, N Carrillo, J L Calvo-Guirado, J Villamil, R A Delgado-Ruíz
The purpose of this study was to analyse the potential of platelet-rich plasma (PRP) culture media to induce osteogenic differentiation of periodontal ligament stem cells and dental pulp stem cells compared with four other methods of culture. Both types of cell were collected from 35 healthy patients and cultured in five different media (Dulbecco's modified eagle's medium (DMEM); DMEM and melatonin; DMEM and PRP; DMEM and ascorbic acid 200μmol; DMEM and l-ascorbate 2-phosphate 50μmol). Cells were characterised by flow cytometry...
June 13, 2017: British Journal of Oral & Maxillofacial Surgery
https://www.readbyqxmd.com/read/28619642/polysaccharides-and-their-depolymerized-fragments-from-costaria-costata-molecular-weight-and-sulfation-dependent-anticoagulant-and-fgf-fgfr-signal-activating-activities
#12
Ningning Hou, Meng Zhang, Yingjie Xu, Zhongmin Sun, Jing Wang, Lijuan Zhang, Quanbin Zhang
Crude polysaccharides from Costaria costata were extracted by hot water and further fractionated by anion exchange chromatography into three polysaccharide fractions. Three low molecular weight fragments were then prepared by degradation of the polysaccharides with hydrogen peroxide and ascorbic acid. The structural features of the polysaccharides and their low molecular weight fragments were elucidated for the first time based on the HGPC, FT-IR, NMR, MS, monosaccharide composition, and other chemical analyses...
June 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28616667/fluorescence-turn-on-detection-of-alkaline-phosphatase-activity-based-on-controlled-release-of-pei-capped-cu-nanoclusters-from-mno2-nanosheets
#13
Yunyi Zhang, Yongxin Li, Cuiyun Zhang, Qingfeng Zhang, Xinan Huang, Meiding Yang, Sohail Anjum Shahzad, Kenneth Kam-Wing Lo, Cong Yu, Shichun Jiang
A fluorescence turn-on assay for alkaline phosphatase (ALP) activity is developed through the controlled release of polyethyleneimine-capped copper nanoclusters (PEI-capped CuNCs) from the MnO2 nanosheets. In an aqueous solution, the positively charged PEI-capped CuNCs could be adsorbed onto the surface of the negatively charged MnO2 nanosheets. Such adsorption through favorable electrostatic interactions could efficiently quench the nanocluster fluorescence emission via resonance energy transfer from the PEI-capped CuNCs to the MnO2 nanosheets...
June 14, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28615661/increased-levels-of-ascorbic-acid-in-the-cerebrospinal-fluid-of-cognitively-intact-elderly-patients-with-major-depression-a-preliminary-study
#14
Kenji Hashimoto, Tamaki Ishima, Yasunori Sato, Davide Bruno, Jay Nierenberg, Charles R Marmar, Henrik Zetterberg, Kaj Blennow, Nunzio Pomara
Major depressive disorder (MDD) in the elderly is a risk factor for dementia, but the precise biological basis remains unknown, hampering the search for novel biomarkers and treatments. In this study, we performed metabolomics analysis of cerebrospinal fluid (CSF) from cognitively intact elderly patients (N = 28) with MDD and age- and gender-matched healthy controls (N = 18). The CSF levels of 177 substances were measured, while 288 substances were below the detection limit. Only ascorbic acid was significantly different, with higher levels in the MDD group at baseline...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28614963/watermelon-juice-concentration-using-ultrafiltration-analysis-of-sugar-and-ascorbic-acid
#15
Chiranjit Bhattacharjee, Vinod K Saxena, Suman Dutta
Raw watermelon juice was concentrated in a laboratory-scale flat-plate ultrafiltration system incorporating polyethersulfone membranes with a molecular weight cutoff of 5 kDa. The experiments have been carried out over a wide range of transmembrane pressures (100-300 kPa). The effect of ultrafiltration with molecular weight cutoff of 5 kDa polyethersulfone membrane on concentrate properties is reported. The total soluble solid content of the permeate was found to be very good, while the ascorbic acid content was on the lower side as compared to feed...
January 1, 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/28613281/imaging-metal-like-monoclinic-phase-stabilized-by-surface-coordination-effect-in-vanadium-dioxide-nanobeam
#16
Zejun Li, Jiajing Wu, Zhenpeng Hu, Yue Lin, Qi Chen, Yuqiao Guo, Yuhua Liu, Yingcheng Zhao, Jing Peng, Wangsheng Chu, Changzheng Wu, Yi Xie
In correlated systems, intermediate states usually appear transiently across phase transitions even at the femtosecond scale. It therefore remains an open question how to determine these intermediate states-a critical issue for understanding the origin of their correlated behaviour. Here we report a surface coordination route to successfully stabilize and directly image an intermediate state in the metal-insulator transition of vanadium dioxide. As a prototype metal-insulator transition material, we capture an unusual metal-like monoclinic phase at room temperature that has long been predicted...
June 14, 2017: Nature Communications
https://www.readbyqxmd.com/read/28612399/postharvest-gaseous-ozone-treatment-enhances-quality-parameters-and-delays-softening-in-cantaloupe-melon-during-storage-at-6%C3%A2-%C3%A2-c
#17
Mauro Toti, Cristian Carboni, Rinaldo Botondi
BACKGROUND: This trial shows postharvest gaseous ozone treatment to evaluate the quality parameters and cell wall enzymes during the Cantaloupe melon cv Caldeo storage at 6 °C for 13 days. RESULTS: The fruits were kept in cold storage and treated with 0.15 ppm gaseous ozone during the day and 0.3 ppm overnight; the control fruits (CK) are stored in normal atmosphere. The firmness was higher and ethylene concentration was significantly lower on treated fruits compared to CK (untreated)...
June 14, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28612342/effect-of-glycine-nitrogen-on-lettuce-growth-under-soilless-culture-a-metabolomics-approach-to-identify-the-main-changes-occurred-in-plant-primary-and-secondary-metabolism
#18
Xiao Yang, Lei Feng, Li Zhao, Xiaosong Liu, Danial Hassani, Danfeng Huang
BACKGROUND: Lettuce is a significant source of antioxidants and bioactive compounds. Nitrate is a cardinal fertilizer in horticulture and influences vegetable yield and quality; however, the negative effects of nitrate on the biosynthesis of flavonoids require further study. It is expected that using fertilizers containing organic N could promote the synthesis of health-promoting compounds. RESULTS: Lettuces were hydroponically cultured in media containing 9 mM nitrate or 9 mM glycine for 4 weeks...
June 14, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28610446/impacts-of-pulsed-electric-field-and-heat-treatment-on-quality-and-sensory-properties-and-microbial-inactivation-of-pomegranate-juice
#19
Gulsun A Evrendilek
Synergistic effects of pulsed electric field+mild heat on quality properties of pomegranate juice were modeled using the best-fit multiple (non-) linear regression models with inactivation kinetics parameters of Escherichia coli O157:H7 and Staphylococcus aureus. No significant difference was detected between the control and the treated samples in terms of pH; °Brix; total antioxidant capacity; total monomeric anthocyanin content; total ascorbic acid concentration; and the sensory properties of flavor, taste, aftertaste, and overall acceptance ( p > 0...
January 1, 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/28609715/an-ultrasensitive-photoelectrochemical-immunosensor-for-insulin-detection-based-on-biobr-ag2s-composite-by-in-situ-growth-method-with-high-visible-light-activity
#20
Dawei Fan, Haoyuan Wang, Malik Saddam Khan, Chunzhu Bao, Huan Wang, Dan Wu, Qin Wei, Bin Du
A novel ultrasensitive label-free immunosensor based on BiOBr/Ag2S composite with high visible-light photoelectrochemical activity was prepared for the detection of insulin. After BiOBr was modified by thioglycolic acid, Ag2S nanoparticles were grown in-situ on the surface of BiOBr hierarchical microspheres to first form novel BiOBr/Ag2S composite. When ascorbic acid (AA) was used as an efficient electron donor for scavenging photo-generated holes, BiOBr/Ag2S composite material showed excellent photoelectrochemical activity...
June 10, 2017: Biosensors & Bioelectronics
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