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https://www.readbyqxmd.com/read/28723208/the-triple-roles-of-glutathione-for-a-dna-cleaving-dnazyme-and-development-of-a-fluorescent-glutathione-cu-2-dependent-dnazyme-sensor-for-detection-of-cu-2-in-drinking-water
#1
Shijin Wang, Chengcheng Liu, Guiying Li, Yongjie Sheng, Yanhong Sun, Hongyue Rui, Jin Zhang, Jiacui Xu, Dazhi Jiang
Pistol-like DNAzyme (PLDz) is an oxidative DNA-cleaving catalytic DNA with ascorbic acid as cofactor. Herein, glutathione was induced into the reaction system to maintain reduced ascorbic acid levels for higher efficient cleavage. However, data indicated that glutathione played triple roles in PLDz-catalyzed reactions. Glutathione alone had no effect on PLDz, and showed inhibitory effect on ascorbic acid-induced PLDz catalysis, but exhibited stimulating effect on Cu(2+)-promoted self-cleavage of PLDz. Further analysis of the effect of glutathione/Cu(2+) on PLDz indicated that H2O2 played a key role in PLDz catalysis...
March 24, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28723118/valence-states-modulation-strategy-for-picomole-level-assay-of-hg-2-in-drinking-and-environmental-water-by-directional-self-assembly-of-gold-nanorods
#2
Lu Chen, Linlin Lu, Sufan Wang, Yunsheng Xia
In this study, we present a valence states modulation strategy for picomole level assay of Hg(2+) using directional self-assembly of gold nanorods (AuNRs) as signal readout. Hg(2+) ions are first controllably reduced to Hg(+) ions by appropriate ascorbic acid, and the reduced Hg(+) ions react with the tips of the preadded AuNRs and form gold amalgam. Such Hg(+) decorated AuNRs then end-to-end self-assemble into one-dimensional architectures by the bridging effects of lysine based on the high affinity of NH2-Hg(+) interactions...
June 23, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28721305/fully-automated-gmp-production-of-68-ga-ga-do3a-vs-cys-40-exendin-4-for-clinical-use
#3
Irina Velikyan, Ulrika Rosenstrom, Olof Eriksson
[(68)Ga]Ga-DO3A-VS-Cys(40)-Exendin-4/PET-CT targeting glucagon like peptide-1 receptor (GLP-1R) has previously demonstrated its potential clinical value for the detection of insulinomas. The production and accessibility of this radiopharmaceutical is one of the critical factors in realization of clinical trials and routine clinical examinations. Previously, the radiopharmaceutical was prepared manually, however larger scale of clinical trials and healthcare requires automation of the production process in order to limit the operator radiation dose as well as improve tracer manufacturing robustness and on-line documentation for enhanced good manufacturing practice (GMP) compliance...
2017: American Journal of Nuclear Medicine and Molecular Imaging
https://www.readbyqxmd.com/read/28720966/biochemical-characterization-and-thermal-inactivation-of-polyphenol-oxidase-from-elephant-foot-yam-amorphophallus-paeoniifolius
#4
Anuradha Singh, Neeraj Wadhwa
Polyphenol oxidase from Amorphophallus corm was purified (5.54 fold) by acetone precipitation and ion exchange chromatography. Electrophoretic-blot analysis revealed that the molecular weight of enzyme is 40 kDa. Enzyme preferred catechol as substrate and showed optimum activity at pH 6 and 35 °C with Km of 17.56 mM and Vmax 0.023 U/ml/min. Thermal inactivation kinetics study showed that 5.68% inactivation was achieved at 75 °C within 40 min. The half-life time of PPO is between 126.00 and 32.54 min...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28720957/bioactive-compounds-and-antioxidant-activity-in-scalded-jalape%C3%A3-o-pepper-industrial-byproduct-capsicum-annuum
#5
Claudia Jaqueline Sandoval-Castro, Maribel Valdez-Morales, B Dave Oomah, Roberto Gutiérrez-Dorado, Sergio Medina-Godoy, L Gabriela Espinosa-Alonso
Bioactive compounds and antioxidant activity were evaluated from industrial Jalapeño pepper byproducts and simulated non processed byproducts from two Mexican states (Chihuahua and Sinaloa) to determine their value added potential as commercial food ingredients. Aqueous 80% ethanol produced about 13% of dry extract of polar compounds. Total phenolic content increased and capsaicin and dihydrocapsaicin decreased on scalding samples (80 °C, 2 min) without affecting ascorbic acid. The major phenolic compounds, rutin, epicatechin and catechin comprised 90% of the total compounds detected by HPLC of each Jalapeño pepper byproducts...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28720943/non-enzymatic-browning-due-to-storage-is-reduced-by-using-clarified-lemon-juice-as-acidifier-in-industrial-scale-production-of-canned-peach-halves
#6
Domingo Saura, Salud Vegara, Nuria Martí, Manuel Valero, José Laencina
Non-enzymatic browning (NEB) in canned peach halves in syrup during storage was investigated. Absorbance at 420 nm (A420), colorimetric parameters (CIELab, TCD and La/b), fructose, glucose and sucrose, total sugar, organic acids, ascorbic acid (AA), dehydroascorbic acid, and 2,3-diketogulonic acid were used to estimate the extent of NEB during 1 year of storage at 30 °C and the relationships between each of these parameters and A420 were established. The investigation was carried out to explore the possibility of replacing the E330 commonly used as acidifier by turbid or clarified lemon juice (TLJ or CLJ) to obtain a product having good nutrition with better retention of quality...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28720375/protective-role-of-antioxidant-compounds-against-peroxynitrite-mediated-modification-of-r54c-mutant-%C3%AE-a-crystallin
#7
Kazem Khoshaman, Reza Yousefi, Ali Akbar Moosavi-Movahedi
As a highly potent reactive oxygen and nitrogen species, peroxynitrite (PON) has endogenous production in the eye ball and contributes to a variety of ocular disorders. In the current study the structural characteristics, chaperone-like activity and conformational stability of R54C mutant αA-crystallin (αA-Cry) were studied upon modification with PON and in the presence of three antioxidant compounds such as ascorbic acid (ASA), glutathione (GSH) and N-acetylcysteine (NAC) using gel electrophoresis and different spectroscopy methods...
July 15, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28717606/combined-supplementation-of-ascorbic-acid-and-thyroid-hormone-t3-affects-tenocyte-proliferation-the-effect-of-ascorbic-acid-in-the-production-of-nitric-oxide
#8
Viviana di Giacomo, Martina Berardocco, Marialucia Gallorini, Francesco Oliva, Alessia Colosimo, Amelia Cataldi, Nicola Maffulli, Anna C Berardi
BACKGROUND: Tissue engineering is now increasingly focusing on cell-based treatments as promising tools to improve tendon repair. However, many crucial aspects of tendon biology remain to be understood before adopting the best experimental approach for cell-tissue engineering. METHODS: The role played by Ascorbic Acid (AA) alone and in combination with thyroid hormone T3 in the viability and proliferation of primary human tendon-derived cells was investigated. Human tenocyte viability was detected by Trypan blue exclusion test and cellular proliferation rate was evaluated by CFSE CellTrace™...
January 2017: Muscles, Ligaments and Tendons Journal
https://www.readbyqxmd.com/read/28717544/is-it-safe-to-prescribe-ascorbic-acid-for-urinary-acidification-in-stone-forming-patients-with-alkaline-urine
#9
Yasser A Noureldin, Alexandrine da Silva, Nader Fahmy, Sero Andonian
OBJECTIVE: To study the effect of ascorbic acid (AA) supplementation on urinary pH, metabolic stone workup parameters, and development of de novo urolithiasis in stone-forming patients. MATERIAL AND METHODS: A retrospective review of the patients followed-up at a tertiary stone centre between September 2009 and October 2015 was performed. Patients with recurrent urolithiasis who received AA supplementation as a urinary acidifying agent were included in the study...
June 2017: Turkish Journal of Urology
https://www.readbyqxmd.com/read/28717330/an-experimental-evaluation-of-adaptogenic-potential-of-standardized-epipremnum-aureum-leaf-extract
#10
Sreemoy Kanti Das, Pinaki Sengupta, Mohd Shahimi Mustapha, Md Moklesur Rahman Sarker
BACKGROUND: Stress is a normal part of everyday life but chronic stress can lead to a variety of stress-related illnesses including hypertension, anxiety, and depression. In the present investigation, standardized leaf extract of Epipremnumaureum was evaluated for its anti-stress potential. MATERIALS AND METHODS: For the evaluation of anti-stress activity, groups of mice (n = 6) were subjected to forced swim stress and anoxic stress tolerance test in mice 1h after daily treatment of E...
April 2017: Journal of Pharmacy & Bioallied Sciences
https://www.readbyqxmd.com/read/28716419/a-%C3%AE-carotene-desaturase-gene-ibzds-increases-%C3%AE-carotene-and-lutein-contents-and-enhances-salt-tolerance-in-transgenic-sweetpotato
#11
Ruijie Li, Chen Kang, Xuejin Song, Ling Yu, Degao Liu, Shaozhen He, Hong Zhai, Qingchang Liu
ζ-Carotene desaturase (ZDS) is one of the key enzymes in carotenoid biosynthesis pathway. However, the ZDS gene has not been applied to carotenoid improvement of plants. Its roles in tolerance to abiotic stresses have not been reported. In this study, the IbZDS gene was isolated from storage roots of sweetpotato (Ipomoea batatas (L.) Lam.) cv. Nongdafu 14. Its overexpression significantly increased β-carotene and lutein contents and enhanced salt tolerance in transgenic sweetpotato (cv. Kokei No. 14) plants...
September 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28715861/synthesis-of-less-toxic-gold-nanorods-by-using-dodecylethyldimethylammonium-bromide-as-an-alternative-growth-directing-surfactant
#12
Joshua M Allen, Junpeng Xu, Maria Blahove, Stephanie A Canonico-May, Taylor J Santaloci, Martha E Braselton, John W Stone
Gold nanoparticles having a rod-like morphology are regularly investigated as potential nano-therapeutic materials owing to their interesting optical properties, facile surface modification, tunable aspect ratios, and low cytotoxicity. Gold nanorods are historically prepared starting from HAuCl4 in the presence of ascorbic acid, silver nitrate, and the growth directing surfactant, cetyltrimethylammonium bromide (CTAB). While CTAB drives a rod-like morphology, it is known to be cytotoxic. This inherent toxicity is often addressed by removing or masking the native CTAB surfactant present on the nanorod surface...
July 1, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28714345/chemical-composition-and-antioxidant-activity-of-aerial-parts-of-ferula-longipes-coss-ex-bonnier-and-maury
#13
Aicha Bouratoua, Assia Khalfallah, Chawki Bensouici, Zahia Kabouche, Abdulmagid Alabdul Magid, Dominique Harakat, Laurence Voutquenne-Nazabadioko, Ahmed Kabouche
This is the first study on the phytochemistry and antioxidant activity of Ferula longipes Coss. ex Bonnier and Maury (Apiaceae). A new flavonoid quercetin-3-O-α-L-rhamnopyranoside-7-O-ß-D-[2-O-caffeoyl]-glucopyranoside (1), along with 10 known compounds kaempferol-3-O-α-L-rhamnopyranoside (2), quercetin-3-O-α-L-rhamnopyranoside (3), kaempferol-3-O-ß-D-glucopyranoside-7-O-α-L-rhamnopyranoside (4), isorhamnetin-3-O-α-L-rhamnopyranoside-7-O-ß-D-glucopyranoside (5), quercetin-3-O-α-L-rhamnopyranoside-7-O-ß-D-glucopyranoside (6), isorhamnetin-3,7-di-O-β-D-glucopyranoside (7), apigenin (8), apigenin-7-O-ß-D-glucopyranoside (9), 3,5-dicaffeoylquinic acid (10), deltoin (11) were isolated from the aerial parts of Ferula longipes Coss...
July 16, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28714237/effects-of-intracameral-ascorbic-acid-on-the-corneal-endothelium-of-dogs-undergoing-phacoemulsification
#14
Ivan R M Padua, Gisele P Valdetaro, Tiago B Lima, Karina K Kobashigawa, Paloma E S Silva, Marcela Aldrovani, Pedro P M Padua, Jose L Laus
OBJECTIVE: Cataracts are the most common ocular disorder in dogs. Phacoemulsification is the preferred treatment method among ophthalmologists, but the cellularity of the endothelium must be considered for its success, as endothelial lesions may produce permanent corneal decompensation. The objective of this study was to evaluate the effects of intracameral ascorbic acid, a known antioxidant, on the corneal endothelium of dogs undergoing phacoemulsification. ANIMAL STUDIED: In all, 40 eyes from 20 dogs, males and females from 7 to 12 years of age, were assessed for mature cataracts...
July 17, 2017: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/28713028/the-effect-of-fusarium-culmorum-infection-and-deoxynivalenol-don-application-on-proteome-response-in-barley-cultivars-chevron-and-pedant
#15
Klára Kosová, Jana Chrpová, Jiří Šantrůček, Radovan Hynek, Lenka Štěrbová, Pavel Vítámvás, Jana Bradová, Ilja Tom Prášil
Fusarium head blight (FHB) disease adversely affects grain quality and final yield in small-grain cereals including barley. In the present study, the effect of an artificial infection with Fusarium culmorum and an application of deoxynivalenol (DON) on barley spikes of cultivars Chevron and Pedant during flowering was investigated at grain mid-dough stage (BBCH 73) 10days after pathogen inoculation (10 dai). Proteomic analysis using a two-dimensional differential gel electrophoresis (2D-DIGE) technique coupled with LC-MS/MS investigated 98 protein spots revealing quantitative or qualitative differences between the experimental variants...
July 13, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28712076/nickel-stressed-responses-of-rice-in-ni-subcellular-distribution-antioxidant-production-and-osmolyte-accumulation
#16
Muhammad Rizwan, Muhammad Imtiaz, Zhihua Dai, Sajid Mehmood, Muhammad Adeel, Jinchang Liu, Shuxin Tu
Nickel has been found a key pollutant in farmlands of central and south China, and understanding of Ni toxicity in rice is of great significance in safety production of rice and remediation of Ni polluted paddy soils. The present study aimed to investigate the uptake and subcellular distribution of Ni, antioxidant production, and osmolyte accumulation of rice (Oryza sativa L., cv. yangliangyou 6) plants exposed to excessive Ni concentrations to gain an insight into Ni-induced phytotoxicity. Results revealed that exposure of rice seedlings to high Ni concentrations resulted a decline in root and shoot lengths and fresh weight (FW) and dry weight (DW) of rice plants, which are in connection with the depletion of the contents of photosynthetic pigments...
July 15, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28711996/the-physiological-and-metabolic-changes-in-sugar-beet-seedlings-under-different-levels-of-salt-stress
#17
Yuguang Wang, Piergiorgio Stevanato, Lihua Yu, Huijie Zhao, Xuewei Sun, Fei Sun, Jing Li, Gui Geng
Salinity stress is a major limitation to global crop production. Sugar beet, one of the world's leading sugar crops, has stronger salt tolerant characteristics than other crops. To investigate the response to different levels of salt stress, sugar beet was grown hydroponically under 3 (control), 70, 140, 210 and 280 mM NaCl conditions. We found no differences in dry weight of the aerial part and leaf area between 70 mM NaCl and control conditions, although dry weight of the root and whole plant treated with 70 mM NaCl was lower than control seedlings...
July 15, 2017: Journal of Plant Research
https://www.readbyqxmd.com/read/28711978/the-activity-of-the-antioxidant-defense-system-of-the-weed-species-senna-obtusifolia-l-and-its-resistance-to-allelochemical-stress
#18
Érica Marusa Pergo Coelho, Mauro Cezar Barbosa, Márcio Shigueaki Mito, Gislaine Cristiane Mantovanelli, Rubem Silvério Oliveira, Emy Luiza Ishii-Iwamoto
Senna obtusifolia L., a common weed in the tropical and subtropical regions of the world, is able to germinate under adverse environmental conditions, suggesting that this species has efficient stress-adaptation strategies. The aims of the present work were to examine the energy metabolism and the antioxidant defense system of the Senna obtusifolia L. during seed germination and initial growth, and the responses to allelochemical-induced stress. Respiratory activity, the activities of alcohol dehydrogenase (ADH), superoxide dismutase (SOD), catalase (CAT),guaicol peroxidase (POD), ascorbate peroxidase (APX), glutathione reductase (GR), lipoxygenase (LOX) and the content of malondialdehyde (MDA) and glutathione (GSSG and GSH) were measured...
July 15, 2017: Journal of Chemical Ecology
https://www.readbyqxmd.com/read/28711840/a-promising-protected-ascorbic-acid-hydroxyapatite-nanocomposite-as-a-skin-anti-ager-a-detailed-photo-and-thermal-stability-study
#19
Mahmoud A Sliem, Rania A Karas, M A Harith
A new ascorbic acid (AA) nanocomposite with low toxicity and high photo and thermal stability is constructed for certain dermatological applications in humans. The presented nanocomposite consists of AA, nano-hydroxyapatite (nHAp) and carboxymethyl cellulose (CMC). The physicochemical properties of such CMC-nHAp-AA nanocomposite were characterized using X-Ray diffractometery (XRD), Fourier Transform Infra-Red (FTIR), Energy Dispersive X-ray (EDX) and UV-VIS spectroscopies. The size and morphology of the synthesized nanocomposites were characterized by TEM/SEM techniques...
July 8, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28710950/citric-acid-assisted-phytoextraction-of-chromium-by-sunflower-morpho-physiological-and-biochemical-alterations-in-plants
#20
Mujahid Farid, Shafaqat Ali, Muhammad Rizwan, Qasim Ali, Farhat Abbas, Syed Asad Hussain Bukhari, Rashid Saeed, Longhua Wu
Soil and water contamination from heavy metals and metalloids is one of the most discussed and burning global issues due to its potential to cause the scarcity of healthy food and safe water. The scientific community is proposing a range of lab and field based physical, chemical and biological solutions to remedy metals and metalloids contaminated soils and water. The present study finds out a possibility of Chromium (Cr) extraction by sunflower from spiked soil under chelating role of citric acid (CA). The sunflower plants were grown under different concentrations of Cr (0, 5, 10 & 20mgkg(-1)) and CA (0, 2...
July 12, 2017: Ecotoxicology and Environmental Safety
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