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https://www.readbyqxmd.com/read/28544921/in-situ-formed-copper-nanoparticles-templated-by-tdt-mediated-dna-for-enhanced-spr-sensor-based-dna-assay
#1
Pei-Xin Yuan, Sheng-Yuan Deng, Chen-Yu Zheng, Serge Cosnier, Dan Shan
For the efficient surface plasmon resonance (SPR)-based DNA assay researching, signal amplification tactics were absolutely necessary. In this work, a sensitive SPR-DNA sensor was developed by employing in situ synthesis of copper nanoparticles (CuNPs) templated by poly-T sequences DNA from terminal deoxynucleotidyl transferase (TdT)-mediated extension, and synergistically with nano-effect deposition as the mass relay. The objective of this strategy was manifold: firstly, tDNA hybridized with the optimal designed probes to active the TdT-mediated DNA extension onto the surface of SPR chip, resulted a long poly-T sequences ssDNA chain in dsDNA terminal onto surface of gold chip and characterized by SPR signal amplitudes...
May 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28544513/composition-antioxidant-and-anticancer-activities-of-the-essential-oil-from-fresh-and-air-dried-aerial-parts-of-pallenis-spinosa
#2
Mahmoud A Al-Qudah, Ayman M Saleh, Naif L Alhawsawi, Hala I Al-Jaber, Syed A Rizvi, Fatma U Afifi
This study was performed to determine the chemical composition, antioxidant and anticancer effects of essential oils extracted from the aerial parts of fresh (F-PSEO) and air-dried (D-PSEO) Pallenis spinosa. The composition of the oils was analyzed by GC and GC-MS, the antioxidant activity by free radical scavenging and metal chelating assays, and their cytotoxicity by a flow cytometry analysis. The primary components in both oils were sesquiterpene hydrocarbons and oxygentated sesquiterpenes. F-PSEO contained 36 different compounds; α-cadinol (16...
May 23, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28544013/-epigallocatechin-3-gallate-inhibits-human-angiotensin-converting-enzyme-activity-through-an-autoxidation-dependent-mechanism
#3
Zhe Liu, Sayaka Nakashima, Toshiyuki Nakamura, Shintaro Munemasa, Yoshiyuki Murata, Yoshimasa Nakamura
We investigated the molecular mechanisms involved in the angiotensin-converting enzyme (ACE) inhibition by (-)-epigallocatechin-3-gallate (EGCg), a major tea catechin. EGCg inhibited both the ACE activity in the lysate of human colorectal cancer cells and human recombinant ACE (rh-ACE) in a dose-dependent manner. Co-incubation with zinc sulfate showed no influence on the rh-ACE inhibition by EGCg, whereas it completely counteracted the inhibitory effect of ethylenediaminetetraacetic acid, a chelating-type ACE inhibitor...
May 23, 2017: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/28539282/-effect-of-polycaprolactone-ascobic-acid-scaffold-in-repairing-articular-cartilage-defects-in-rabbits
#4
Zhi-Hui Huang, Bing Song, Yu-Fan Chen, Zhe-Ting Liao, Liang Zhao
OBJECTIVE: To investigate the effect of polycaprolactone-ascobic acid (PCL-AA) scaffolds in promoting repair of articular cartilage defects in a rabbit model. METHODS: The cartilage defects (3.5 mm in diameter and 3.0 mm in depth) were created in the trochlear groove of the bilateral knees of eight 6-month-old male New Zealand white rabbits. The rabbit models were then randomized into 3 groups to receive implantation of PCL-AA scaffolds (group A, n=8), implantation of PCL scaffolds without AA (group B, n=5), or no treatment (group C, n=3)...
May 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28539233/supplementation-action-with-ascorbic-acid-in-the-morphology-of-the-muscular-layer-and-reactive-acetylcholinesterase-neurons-of-ileum-of-mdx-mice
#5
Marcelo José Santiago Lisboa, Marília Fabiana De Oliveira Lima, Sandra Regina Stabille, Jacqueline Nelisis Zanoni, Karina Martinez Gagliardo, Melyna Soares Souto, Renivaldo Souza, Jodonai Barbosa Da Silva, Sônia Regina De Almeida Yokomizo, Edson Aparecido Liberti, Naianne Kelly Clebis
The Duchenne Muscular Dystrophy (DMD) is a genetic disorder characterized by the absence of dystrophin protein, causing severe myopathy from increases of oxidative stress. Injuries of intestinal muscle can compromise the myenteric plexus. This study aimed to evaluate the disorders occurred in the muscular layer and in the acetylcholinesterase myenteric neurons (ACHE-r) of ileum of mdx mice, and the effects of supplementation with ascorbic acid (AA) in both components. 30 male mice C57BL/10, and 30 male mice C57BL/10Mdx were separated according to the age and treatment (n=10/group): 30-days-old control group (C30); 30-days-old dystrophic group (D30); 60-days-old control group (C60); 60-days-old dystrophic group (D60); 60-days-old control group supplemented with AA (CS60); and 60-days-old dystrophic group supplemented with AA (DS60)...
May 17, 2017: Autonomic Neuroscience: Basic & Clinical
https://www.readbyqxmd.com/read/28536360/coconut-cocos-nucifera-ethanolic-leaf-extract-reduces-amyloid-%C3%AE-1-42-aggregation-and-paralysis-prevalence-in-transgenic-caenorhabditis-elegans-independently-of-free-radical-scavenging-and-acetylcholinesterase-inhibition
#6
Rafael Vincent Manalo, Maries Ann Silvestre, Aza Lea Anne Barbosa, Paul Mark Medina
Virgin coconut oil (VCO) has been the subject of several studies which have aimed to alleviate Alzheimer's disease (AD) pathology, focusing on in vitro antioxidant and acetylcholinesterase (AChE) inhibitory activities. Here, we studied an underutilized and lesser-valued part of the coconut tree, specifically the leaves, using in vitro and in vivo approaches. Coconut leaf extract (CLE) was screened for antioxidant and AChE inhibitory properties in vitro and therapeutic effects in two strains of transgenic Caenorhabditis elegans expressing amyloid-β1-42 (Aβ1-42) in muscle cells...
April 21, 2017: Biomedicines
https://www.readbyqxmd.com/read/28536020/%C3%B0-valuation-of-biocompatibility-and-antioxidant-efficiency-of-chitosan-alginate-nanoparticles-loaded-with-quercetin
#7
Denitsa Aluani, Virginia Tzankova, Magdalena Kondeva-Burdina, Yordan Yordanov, Elena Nikolova, Feodor Odzhakov, Alexandar Apostolov, Tzvetanka Markova, Krassimira Yoncheva
The present study deals with development and evaluation of the safety profile of chitosan/alginate nanoparticles as a platform for delivery of a natural antioxidant quercetin. The nanoparticles were prepared by varying the ratios between both biopolymers giving different size and charge of the formulations. The biocompatibility was explored in vitro in cells from different origin: cultivated HepG2 cells, isolated primary rat hepatocytes, isolated murine spleen lymphocytes and macrophages. In vivo toxicological evaluation was performed after repeated 14-day oral administration to rats...
May 20, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28535610/nitric-oxide-inhibition-and-procollagen-type-i-peptide-synthesis-of-a-phenolic-amide-identified-from-the-stem-of-lycium-chinense-miller
#8
Chan Saem Gil, Moon-Sik Chang, Seok Hyun Eom
The bioactivities of boxthron fruits, a source of oriental medicine, are known, while phytochemical studies of the boxthorn stem are rare. In this study, the stem extract of boxthorn (Lycium chinense Miller) and its sub-fractions were evaluated for their effects on nitric oxide (NO) inhibition and procollagen type I peptide (PIP) synthesis. A phenolic amide isolated from the stem extract was also assayed for these effects. The compound, N-trans-feruloyltyramine, was identified by (1)H, (13)C, and 2D-nuclear magnetic resonance analysis...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28534307/acanthus-ilicifolius-l-a-promising-candidate-for-phytostabilization-of-zinc
#9
A M Shackira, Jos T Puthur, E Nabeesa Salim
The potential of a halophyte species-Acanthus ilicifolius L.-to phytostabilize zinc (Zn) grown under hydroponics culture conditions was critically evaluated in this study. The propagules after treating with ZnSO4 (4 mM) were analysed for the bioaccumulation pattern, translocation rate of Zn to the shoot, effects of Zn accumulation on organic solutes and the antioxidant defence system. It was found that most of the Zn absorbed by the plant was retained in the root (47%) and only a small portion was transported to stem (12%) and leaves (11%)...
June 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28532060/design-and-characterization-of-biodegradable-macroporous-hybrid-inorganic-organic-polymer-for-orthopedic-applications
#10
Sunita PremVictor, Jibin Kunnumpurathu, M G Gayathri Devi, K Remya, Vineeth M Vijayan, Jayabalan Muthu
We have engineered hybrid polymer products based on a hybrid inorganic-organic comacromer consisting of hydroxyapatite (HA), carboxyl terminated polypropylene fumarate (CTPPF), PEG300 and ascorbic acid (AA) as a bone graft material. The integration and the spatial distribution of HA in the polymer backbone were facilitated by silanisation and 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) coupling technique. These comacromers and crosslinked polymer products were characterized by Fourier transform infrared spectroscopy (FTIR), Nuclear magnetic resonance (NMR), Scanning electron microscopy (SEM) and Raman mapping techniques...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28532016/electrochemical-preparation-of-two-nanostructured-poly-sulfosalicylic-acid-films-with-different-morphologies-and-properties-for-selective-sensing-of-dopamine-comparative-study
#11
Juan Zhang, Jun-Ping Zhou, Xiao-Hui Dai, Guang-Zhou Liu, Shu-Yong Zhang
Two nanostructured poly(sulfosalicylic acid) (PSA) films were synthesized from room temperature ionic liquid (RTIL) or aqueous solution on a glassy carbon electrode (GCE) via potentiodynamic electropolymerization. The morphology and properties of the PSA films were characterized with scanning electron microscopy (SEM), scanning probe microscopy (SPM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). It was found that solvent had a major influence on the morphology and electrochemical properties of the resultant PSA films...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28530616/effect-of-ultrasound-treatment-on-visual-color-vitamin-c-total-phenols-and-carotenoids-content-in-cape-gooseberry-juice
#12
Luis Eduardo Ordóñez-Santos, Jader Martínez-Girón, Maria Enith Arias-Jaramillo
Strong interest of consumers in acquiring minimally processed foods that conserve the different micronutrients has raised the need to study the effect of food processing methods on quality attributes. The aim of the study was to determine the effect ultrasound treatment on color, and the bioactive compounds (ascorbic acid, total phenols, carotenoids, and provitamin A) of Cape gooseberry juice. Color values, ascorbic acid, total phenols, carotenoids, and Retinol Activity Equivalent (RAE) were measured. The results indicate significant reductions (p<0...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530612/the-mechanism-of-deterioration-of-the-glucosinolate-myrosynase-system-in-radish-roots-during-cold-storage-after-harvest
#13
Jeong Gu Lee, Sooyeon Lim, Jongkee Kim, Eun Jin Lee
The hydrolysis of glucosinolates (GSLs) by myrosinase yields varieties of degradation products including isothiocyanates (ITCs). This process is controlled by the glucosinolate-myrosinase (G-M) system. The major ITCs in radish roots are raphasatin and sulforaphene (SFE), and the levels of these compounds decrease during storage after harvest. We investigated the G-M system to understand the mechanism behind the decrease in the ITCs in radish roots. Six varieties of radish roots were stored for 8weeks at 0-1...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530571/novel-insights-into-ascorbate-retention-and-degradation-during-the-washing-and-post-harvest-storage-of-spinach-and-other-salad-leaves
#14
Rebecca A Dewhirst, Graham J J Clarkson, Steve D Rothwell, Stephen C Fry
Post-harvest treatments of pre-packaged salad leaves potentially cause l-ascorbate loss, but the mechanisms of ascorbate degradation remain incompletely understood, especially in planta. We explored the extent and pathways of ascorbate loss in variously washed and stored salad leaves. Ascorbate was assayed by 2,6-dichlorophenolindophenol titration, and pathways were monitored by (14)C-radiolabelling followed by high-voltage electrophoresis. All leaves tested showed ascorbate loss during storage: lettuce showed the greatest percentage loss, wild rocket the least...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28528861/mechanism-of-melatonin-protection-against-copper-ascorbate-induced-oxidative-damage-in-vitro-through-isothermal-titration-calorimetry
#15
Arnab K Ghosh, Shamreen Naaz, Bharati Bhattacharjee, Nirajan Ghosal, Aindrila Chattopadhyay, Souvik Roy, Russel J Reiter, Debasish Bandyopadhyay
AIMS: Involvement of oxidative stress in cardiovascular diseases is well established. Melatonin's role as an antioxidant and free radical scavenger via its receptor dependent and receptor independent pathways is well known. The aim of this study is to identify and elaborate upon a third mechanism by which melatonin is able to abrogate oxidative stress. MAIN METHODS: Oxidative stress was induced in vitro, by copper (0.2mM)-ascorbate (1mM) in isolated goat heart mitochondria, cytosol and peroxisomes and they were co-incubated with graded doses of melatonin...
May 18, 2017: Life Sciences
https://www.readbyqxmd.com/read/28527991/lignosulfonate-stabilized-selenium-nanoparticles-and-their-deposition-on-spherical-silica
#16
Anna Modrzejewska-Sikorska, Emilia Konował, Łukasz Klapiszewski, Grzegorz Nowaczyk, Stefan Jurga, Teofil Jesionowski, Grzegorz Milczarek
We report a novel room-temperature synthesis of selenium nanoparticles, which for the first time uses lignosulfonate as a stabilizer. Various lignosulfonates obtained both from hardwood and softwood were tested. Selenium oxide was used as the precursor of zero-valent selenium. Three different reducers were tested - sodium borohydride, hydrazine and ascorbic acid - and the latter proved most effective in terms of the particle size and stability of the final colloid. The lignosulfonate-stabilized selenium nanoparticles had a negative zeta potential, dependent on pH, which for some lignosulfonates reached -50mV, indicating the excellent stability of the colloid...
May 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28527047/dietary-ascorbic-acid-reduced-micronucleus-and-nuclear-abnormalities-in-clarias-gariepinus-burchell-1822-exposed-to-hospital-effluent
#17
Chibuisi G Alimba, Raphael D Ajiboye, Olakunle S Fagbenro
Hospital effluents contain myriad of mutagens and genotoxins capable of increasing DNA damage in aquatic biota. African mudfish, Clarias gariepinus, are exposed to genotoxins when cultured in swamps and derelict water bodies often contaminated by effluents. Moreover, its DNA is susceptible to xenobiotic-induced lesions since it lacks L-gulonolactone oxidase and hence cannot synthesize L-ascorbic acid. This study investigated 96-h acute toxicity and protective effects of dietary ascorbic acid (AA) against micronucleus (MN) and abnormal nuclear (NAs) formation in C...
May 19, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28526602/palmitoyl-ascorbate-and-doxorubicin-co-encapsulated-liposome-for-synergistic-anticancer-therapy
#18
Yue Yang, Xiaoyu Lu, Qi Liu, Yu Dai, Xiaojie Zhu, Yanli Wen, Jiaqiu Xu, Yang Lu, Di Zhao, Xijing Chen, Ning Li
Combination therapy with two drugs and nanoparticle-based drug delivery systems are widely applied to reduce the adverse effects of traditional treatment by chemotherapeutic drugs. Palmitoyl ascorbate (PA) as a lipophilic derivative of ascorbic acid shows the advantages in cancer treatment. The aim of the study was to prepare a doxorubicin (DOX) and PA co-loaded liposome to synergistically treat tumor and effectively alleviate the toxicity caused by DOX. The effects were evaluated by in vitro and in vivo studies...
May 17, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28526347/preparation-of-chitosan-ferulic-acid-conjugate-structure-characterization-and-in-the-application-of-pharmaceuticals
#19
Chen Li, Jian-Bin Li
A novel drug delivery system based on chitosan derivatives was prepared by introducting ferulic acid to chitosan adopting a free radical-induced grafting procedure. This paper used an ascorbic acid/hydrogen peroxide redox pair as radical initiator. The chitosan derivative was characterized by Fourier transformed infrared (FTIR), Ultraviolet-visible spectrum (UV), Differential scanning calorimetry (DSC), X-ray diffraction (XRD) and Electron microscopic scanning (SEM). What is more, preparing microcapsules with the chitosan conjugate as wall material, the drug release propertie of chitosan conjugates were compared with that of a blank chitosan, which treated in the same conditions but in the absence of ferulic acid...
May 16, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28522390/mango-kernel-starch-as-a-novel-edible-coating-for-enhancing-shelf-life-of-tomato-solanum-lycopersicum-fruit
#20
Anjum Nawab, Feroz Alam, Abid Hasnain
Mango kernel starch (MKS) coatings containing different plasticizers were used to extend the shelf life of tomato. The coating slurry was prepared by gelatinizing 4% mango kernel starch, plasticized with glycerol, sorbitol and their 1:1 mixture (50% of starch weight; db). The samples were kept at room temperature (20°C) and analyzed for shelf life. Significant difference in coated and control fruits were observed and all the coated fruits delayed ripening process that was characterized by reduction in weight loss and restricted changes in soluble solids concentration, titratable acidity, ascorbic acid content, firmness and decay percentage compared to uncoated sample...
May 15, 2017: International Journal of Biological Macromolecules
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