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diabetic foot aureus

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https://www.readbyqxmd.com/read/28718238/bacteriology-of-diabetic-foot-in-tertiary-care-hospital-frequency-antibiotic-susceptibility-and-risk-factors
#1
Syeda Sadia Amjad, Jamal Zafar, Nadia Shams
BACKGROUND: Diabetic foot being one of the frequent and disabling complications of diabetes. In view of widespread regional variation in causative organisms and antimicrobial susceptibility, the current study aimed to determine frequency of causative organisms, their antimicrobial susceptibility and associated risk factors. METHODS: This descriptive cross-sectional study was conducted in 6 months' duration at dept. of Medicine; PIMS Hospital Islamabad. Type 2 Diabetes mellitus patients with diabetic foot ulcer were enrolled after informed consent...
April 2017: Journal of Ayub Medical College, Abbottabad: JAMC
https://www.readbyqxmd.com/read/28716100/quality-of-life-and-clinical-radiological-long-term-results-after-implant-associated-infections-in-patients-with-ankle-fracture-a-retrospective-matched-pair-study
#2
Patrick Ziegler, Donat Schlemer, Ingo Flesch, Sonja Bahrs, Ulrich Stoeckle, Sebastian Werner, Christian Bahrs
BACKGROUND: Ankle fractures are frequently occurring injuries. Despite the relatively simple operative technique, patients often suffer from postoperative complications. Little is known about postoperative treatment of implant-associated infections of the ankle. Therefore, this study shows and evaluates a treatment algorithm in long- and short-term outcomes compared to infection-free patients. METHODS: Data from patients of over 20 years of a level 1 trauma center and university hospital was retrospectively analyzed including age, gender, comorbidities, smoking status, fracture classification, number of revisions, length of in-patient stay due to fracture and infection, and results of microbiological specimen with the length of antibiotic treatment...
July 17, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/28682727/bacterial-prevalence-and-antibiotic-resistance-in-clinical-isolates-of-diabetic-foot-ulcers-in-the-northeast-of-tamaulipas-mexico
#3
Mario Sánchez-Sánchez, Wendy Lizeth Cruz-Pulido, Eduardo Bladinieres-Cámara, Rodrigo Alcalá-Durán, Gildardo Rivera-Sánchez, Virgilio Bocanegra-García
Diabetic foot ulcers (DFUs) are a serious and common problem in patients with diabetes mellitus and constitute one of the major causes of lower extremity amputation. The microbiological profile of DFUs depends on the acute or chronic character of the wound. Aerobic gram-positive cocci are the predominant organisms isolated from DFUs. Diabetic foot biopsies from patients admitted to the Angiology and Vascular Surgery Hospital of the Northeast, in Reynosa, Tamaulipas from December 2011 to April 2016 were analyzed...
June 2017: International Journal of Lower Extremity Wounds
https://www.readbyqxmd.com/read/28669650/next-generation-dna-sequencing-of-tissues-from-infected-diabetic-foot-ulcers
#4
M Malone, K Johani, S O Jensen, I B Gosbell, H G Dickson, H Hu, K Vickery
We used next generation DNA sequencing to profile the microbiome of infected Diabetic Foot Ulcers (DFUs). The microbiota was correlated to clinical parameters and treatment outcomes to determine if directed antimicrobial therapy based on conventional microbiological cultures are relevant based on genomic analysis. Patients≥18years presenting with a new Diabetic Foot Infection (DFI) who had not received topical or oral antimicrobials in the two weeks prior to presentation, were eligible for enrolment. Tissue punch biopsies were obtained from infected DFUs for analysis...
June 27, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28667056/endogenous-endophthalmitis-and-osteomyelitis-associated-with-interleukin-17-inhibitor-treatment-for-psoriasis-in-a-patient-with-diabetes
#5
Christine E Martinez, John B Allen, Frederick H Davidorf, Colleen M Cebulla
A 64-year-old man with type 2 diabetes mellitus and plaque psoriasis presented to the emergency room with 3 days of progressive right eye pain and decreased vision. After extensive workup and multidisciplinary team effort, the patient was diagnosed with and treated for unilateral endogenous methicillin-sensitive Staphylococcus aureus endophthalmitis, bacteraemia and osteomyelitis of the foot. The patient had been started on the interleukin 17 (IL-17) inhibitor secukinumab for his treatment-resistant plaque psoriasis 4 weeks prior to presentation...
June 29, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28574021/isolation-of-bacteria-from-diabetic-foot-ulcers-with-special-reference-to-anaerobe-isolation-by-simple-two-step-combustion-technique-in-candle-jar
#6
Jayeeta Haldar, Poulami Mukherjee, Satinath Mukhopadhyay, Prasanta Kumar Maiti
BACKGROUND & OBJECTIVES: Although polymicrobial infections involving both aerobic and anaerobic bacteria are very common in diabetic foot ulcers, in many centres of developing countries, anaerobes are rarely isolated due to technical difficulties. This can be overcome by using a new simple, innovative technique of a combination of candle combustion and use of acidified copper-coated steel wool, as reported here. METHODS: In-house developed method was used in a prospective clinico-microbiological study for anaerobes from randomly selected 43 patients with diabetic foot ulcers along with conventional method of anaerobic culture in GasPak system and aerobic culture by standard laboratory procedures...
January 2017: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/28507348/microbiological-pattern-of-diabetic-foot-infections-at-a-tertiary-care-center-in-a-developing-country
#7
Zahid Miyan, Asher Fawwad, Rubina Sabir, Abdul Basit
OBJECTIVE: To determine the microbiological profile and antibiotic susceptibility patterns of organisms isolated from diabetic foot ulcers in a tertiary care hospital Karachi, Pakistan. METHODS: This descriptive study was conducted at the Baqai Institute of Diabetology and Endocrinology (BIDE), from January 2013 to March 2014. Bone, pus and tissue samples were collected from 342 patients with diabetic foot infections and inoculated on appropriate media. Antibiotic susceptibility tests were done by Kirby Bauer disk diffusion method...
May 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28480287/how-do-preoperative-antibiotics-affect-culture-yield-in-diabetic-foot-infections
#8
Heather Young, Whitney Miller, Randy Burnham, Susan Heard, Chrystal Berg, Timothy C Jenkins
The impact of preoperative antibiotics on culture of diabetic foot infection samples has not been studied. We found that increasing exposure to preoperative antibiotics was associated with less frequent growth of streptococci and anaerobes and more culture-negative results. In contrast, the yield of Staphylococcus aureus and Gram-negative bacilli was unaffected.
2017: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/28480283/using-nuclear-medicine-imaging-wisely-in-diagnosing-infectious-diseases
#9
REVIEW
Andrea Censullo, Tara Vijayan
In recent years, there has been an increasing emphasis on efficient and accurate diagnostic testing, exemplified by the American Board of Internal Medicine's "Choosing Wisely" campaign. Nuclear imaging studies can provide early and accurate diagnoses of many infectious disease syndromes, particularly in complex cases where the differential remains broad. This review paper offers clinicians a rational, evidence-based guide to approaching nuclear medicine tests, using an example case of methicillin-sensitive Staphylococcus aureus (MSSA) bacteremia in a patient with multiple potential sources...
2017: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/28450198/are-antibiotic-resistant-pathogens-more-common-in-subsequent-episodes-of-diabetic-foot-infection
#10
Dan Lebowitz, Karim Gariani, Benjamin Kressmann, Elodie von Dach, Benedikt Huttner, Placido Bartolone, Nam Lê, Morad Mohamad, Benjamin A Lipsky, Ilker Uçkay
BACKGROUND: After antibiotic therapy of an initial diabetic foot infection (DFI), pathogens isolated from subsequent episodes might become more resistant to commonly prescribed antibiotics. If so, this might require a modification of the current recommendations for the selection of empiric antibiotic therapy. This study investigated whether the Infectious Diseases Society of America (IDSA) DFI guideline recommendations should be modified based on the number of past DFI episodes. METHODS: This was a single-centre retrospective cohort survey of DFI patients seen during the years 2010 to 2016...
April 24, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28379101/characteristics-and-clinical-assessment-of-antibiotic-delivery-by-chitosan-sponge-in-the-high-risk-diabetic-foot-a-case-series
#11
N Patel, D Hatch, C M Wells, D Ahn, M Harris, J A Jennings, W Haggard, D G Armstrong
OBJECTIVE: The local delivery of antimicrobials is attractive for a number of reasons. Chitosan, a biodegradable polysaccharide sponge material, has been proposed as medium to deliver antibiotics directly to wounds. In this report we evaluate the safety and practicality of antimicrobial delivery via chitosan sponge. METHOD: We present the clinical course and systemic absorption characteristics of three cases of people with diabetic foot wounds treated with antibiotic soaked chitosan sponge (Sentrex BioSponge, Bionova Medical, Germantown, TN)...
April 1, 2017: Journal of Wound Care
https://www.readbyqxmd.com/read/28361041/decrease-of-staphylococcus-aureus-virulence-by-helcococcus-kunzii-in-a-caenorhabditis-elegans-model
#12
Christelle Ngba Essebe, Orane Visvikis, Marguerite Fines-Guyon, Anne Vergne, Vincent Cattoir, Alain Lecoustumier, Emmanuel Lemichez, Albert Sotto, Jean-Philippe Lavigne, Catherine Dunyach-Remy
Social bacterial interactions are considered essential in numerous infectious diseases, particularly in wounds. Foot ulcers are a common complication in diabetic patients and these ulcers become frequently infected. This infection is usually polymicrobial promoting cell-to-cell communications. Staphylococcus aureus is the most prevalent pathogen isolated. Its association with Helcococcus kunzii, commensal Gram-positive cocci, is frequently described. The aim of this study was to assess the impact of co-infection on virulence of both H...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28326851/evaluation-of-antibacterial-activity-of-the-bark-and-leaf-extracts-of-brosimum-gaudichaudii-tr%C3%A3-cul-against-multidrug-resistant-strains
#13
Joelma da Costa Borges, Michele Cezimbra Perim, Rodrigo Orlandini de Castro, Thiago Antônio de Sousa Araújo, Tadeu José da Silva Peixoto Sobrinho, Ana Carolina Oliveira da Silva, Sandra Maria Botelho Mariano, Solange Cristina Carreiro, Maria Cristina da Silva Pranchevicius
Brosimum gaudichaudii Trécul., a plant that belongs to Moraceae family, is found throughout the Brazilian Cerrado. The antimicrobial activities of ethanolic bark and leaf extracts of B. gaudichaudii were tested against multiresistant bacteria isolated from diabetic foot infections (DFIs). Antimicrobial activity of the extracts was evaluated by agar disc diffusion (DD) and broth dilution (BD) methods. By BD method, bark (53.85, 45.83%) and leaf (42.31, 50.00%) extracts contained antimicrobial activity against both gram-negative and gram-positive bacteria...
March 22, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28219141/-significance-of-bacteria-detection-with-filter-paper-method-on-diagnosis-of-diabetic-foot-wound-infection
#14
X H Zou, Y P Zhu, G Q Ren, G C Li, J Zhang, L J Zou, Z B Feng, B H Li
Objective: To evaluate the significance of bacteria detection with filter paper method on diagnosis of diabetic foot wound infection. Methods: Eighteen patients with diabetic foot ulcer conforming to the study criteria were hospitalized in Liyuan Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology from July 2014 to July 2015. Diabetic foot ulcer wounds were classified according to the University of Texas diabetic foot classification (hereinafter referred to as Texas grade) system, and general condition of patients with wounds in different Texas grade was compared...
February 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28013263/invasive-systemic-infection-after-hospital-treatment-for-diabetic-foot-ulcer-risk-of-occurrence-and-effect-on-survival
#15
Shey-Ying Chen, John M Giurini, Adolf W Karchmer
BACKGROUND: Diabetic foot ulcers (DFUs) threaten limbs and prompt hospitalization. After hospitalization, remote-site invasive systemic infection related to DFU (DFU-ISI) may occur. The characteristics of DFU-ISIs and their effect on mortality risk have not been defined. METHODS: We conducted a retrospective cohort study of 819 diabetic patients hospitalized for treatment of 1212 unique DFUs during a 9-year period. We defined the index ulcer as that present at the first (index) DFU admission to our hospital...
February 1, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/27920754/culture-based-screening-of-aerobic-microbiome-in-diabetic-foot-subjects-and-developing-non-healing-ulcers
#16
Saba Noor, Jamal Ahmad, Iqbal Parwez, Maaz Ozair
The study was carried on diabetic foot patients to deduce clinical attributes, the occurrence of the range of aerobic microbial flora and to assess their comparative in vitro susceptibility to the customarily used antimicrobials. We also studied the potential risk factors involved in the development of non-healing ulcers. A total of 87 organisms were isolated from 70 specimens, including Escherichia coli (19.5%) among the Gram-negative and Staphylococcus aureus (18.4%) among the Gram-positive as the predominant aerobes explored...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27902390/virulence-determinants-in-clinical-staphylococcus-aureus-from-monomicrobial-and-polymicrobial-infections-of-diabetic-foot-ulcers
#17
Kavitha Shettigar, Spoorthi Jain, Deepika V Bhat, Raviraj Acharya, Lingadakai Ramachandra, Kapaettu Satyamoorthy, Thokur Sreepathy Murali
Antibiotic resistance in Staphylococcus aureus is a major public health concern, and methicillin-resistant S. aureus has emerged as an important pathogen. We characterized S. aureus isolates from monomicrobial and polymicrobial wound infections from 200 diabetic individuals with foot ulcers to understand their underlying diversity and pathogenicity. Staphylococcal cassette chromosome mec typing was performed, and genes coding for production of biofilm, Panton-Valentine leukocidin, toxic shock syndrome toxin and leukotoxins DE and M were screened...
December 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27898266/methicillin-resistant-staphylococcus-aureus-in-foot-osteomyelitis
#18
Chester N Ashong, Shazia A Raheem, Andrew S Hunter, Cezarina Mindru, Neal R Barshes
BACKGROUND: Conflicting studies exist regarding the impact of methicillin-resistant Staphylococcus aureus (MRSA) on increased time to wound healing, future need for surgical procedures, and likelihood of treatment failure in patients with diabetic foot osteomyelitis. The purpose of this study is to determine the overall significance of MRSA in predicting treatment failure in bone infections of the foot and to determine an appropriate pre-operative and empiric post-operative antibiotic regimen...
February 2017: Surgical Infections
https://www.readbyqxmd.com/read/27720361/link-between-nasal-carriage-of-staphylococcus%C3%A2-aureus-and-infected-diabetic-foot-ulcers
#19
C Dunyach-Remy, C Courtais-Coulon, C DeMattei, N Jourdan, S Schuldiner, A Sultan, C Carrière, S Alonso, A Sotto, J-P Lavigne
AIMS: Nasal carriage of Staphylococcus aureus in diabetic patients may be a risk factor for diabetic foot lesion infections. The aims of this study were to compare the genotypic profiles of S. aureus strains isolated from nares and diabetic foot ulcers (DFUs) using microarray technology. METHODS: Patients were included if they were admitted for diabetic foot infection (DFI) at any of three diabetology departments of Montpellier and Nîmes University Hospitals between 1 September 2010 to 30 June 2012...
April 2017: Diabetes & Metabolism
https://www.readbyqxmd.com/read/27686306/antimicrobial-susceptibility-of-bacterial-and-fungal-infections-among-infected-diabetic-patients
#20
Ayman Khalid Johargy
Objective: To determine the antimicrobial susceptibility of the most common bacterial and fungal infections among infected diabetic patients. Methods: This study was conducted at the Umm Al-Qura University, Makkah, Saudi Arabia, from June 2011 to June 2012, and comprised specimens collected from diabetics. Antibiotic susceptibility test using disc diffusion method was performed for bacterial isolates, and antifungal susceptibility test using colorimetric method for candida isolates...
October 2016: JPMA. the Journal of the Pakistan Medical Association
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