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https://www.readbyqxmd.com/read/29862868/inhibition-of-mast-cell-degranulation-with-cromolyn-sodium-exhibits-organ-specific-effects-in-polycystic-kidney-pck-rats
#1
Lu Jiang, Pingping Fang, Seth Septer, Udayan Apte, Michele T Pritchard
Autosomal recessive polycystic kidney disease (ARPKD) is a monogenic disease characterized by development of hepatorenal cysts, pericystic fibrosis, and inflammation. Previous studies show that mast cell (MC) mediators such as histamine induce proliferation of cholangiocytes. We observed robust MC accumulation around liver cysts, but not kidney cysts, in polycystic kidney (PCK) rats (an animal model of ARPKD). Therefore, we hypothesized that MCs contribute to hepatic cyst growth in ARPKD. To test this hypothesis, we treated PCK rats with 1 of 2 different MC stabilizers, cromolyn sodium (CS) or ketotifen, or saline...
January 1, 2018: International Journal of Toxicology
https://www.readbyqxmd.com/read/29798127/-effects-of-ketotifen-on-fractional-exhaled-nitric-oxide-in-patients-with-combined-allergic-rhinitis-and-asthma-syndrome
#2
L R Li, Z Y Cui, H C Wang, L X Song, J H Qi, P P Zhang, X Q Han, H Y Wang
Objective: To study effects of ketotifen on fractional exhaled nitric oxide in patients with combined allergic rhinitis and asthma syndrome. Method: One hundred and twelve patients with asthma were selected from department of respiration, Affiliated Hospital of North China University of science and technology from January 2016 to 2017 in March. Eighty-four patients with allergic rhinitis were selected as the subjects, Randomly divided into two groups,including observed group(n=42) and control group(n=42).The control group was treated with antitussive,expectorant and inhaled corticosteroids, while the observed group in the control group combined with ketotifen 1 mg 2/ day oral treatment,the changes of FeNO and IL-17 in the two groups were compared after one month treament...
November 5, 2017: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
https://www.readbyqxmd.com/read/29719638/topical-olopatadine-hydrochloride-versus-ketotifen-fumarate-for-allergic-conjunctivitis
#3
Dharmistha Patel, N Sarala, Narendra Panduranga Datti
Purpose: Allergic conjunctivitis (AC) is associated with itching, redness, tearing, pain, and burning sensation in the eyes. The inflammatory process is caused by the mechanism of immediate hypersensitivity due to direct contact with the allergen. This process triggers mast cells in the conjunctiva to activate and release mediators. The purpose of this study was to compare topical olopatadine and ketotifen in terms of effectiveness and safety for the management of AC. Methods: Patients clinically diagnosed with AC were randomized into two groups of 60 patients each and received either topical olopatadine HCl 0...
April 2018: Journal of Ophthalmic & Vision Research
https://www.readbyqxmd.com/read/29615980/histamine-regulates-molecular-clock-oscillations-in-human-retinal-pigment-epithelial-cells-via-h-1-receptors
#4
Eri Morioka, Yuzuki Kanda, Hayato Koizumi, Tsubasa Miyamoto, Masayuki Ikeda
Vertebrate eyes are known to contain circadian clocks, but their regulatory mechanisms remain largely unknown. To address this, we used a cell line from human retinal pigment epithelium (hRPE-YC) with stable coexpression of reporters for molecular clock oscillations ( Bmal1-luciferase ) and intracellular Ca2+ concentrations ( YC3.6 ). We observed concentration-dependent increases in cytosolic Ca2+ concentrations after treatment with histamine (1-100 µM) and complete suppression of histamine-induced Ca2+ mobilizations by H1 histamine receptor (H1 R) antagonist d -chlorpheniramine ( d -CPA) in hRPE-YC cells...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29600119/a-rapid-extraction-method-to-quantify-drug-uptake-in-contact-lenses
#5
Chau-Minh Phan, Sarah Weber, Jennifer Mueller, Alan Yee, Lyndon Jones
Purpose: To develop a simple extraction procedure to quantify the uptake of four topical ocular pharmaceutical drugs into contact lenses (CLs). Methods: Four silicone hydrogel (SH) CLs (balafilcon A, senofilcon A, lotrafilcon B, comfilcon B) and four conventional hydrogel (CH) CLs (nesofilcon A, hilafilcon B, nelfilcon A, etafilcon A) were evaluated. The drugs studied were natamycin, moxifloxacin, timolol maleate, and ketotifen fumarate. For drug incubation, three CLs of each type were placed in 1 mL of 1 mg/mL drug-loading solution for 24 hours...
March 2018: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/29375080/jte-852-a-novel-spleen-tyrosine-kinase-inhibitor-blocks-antigen-induced-allergic-reactions-in-rats
#6
Toshinobu Kato, Takeshi Ohta, Hidenori Iwasaki, Hatsue Kobayashi, Akira Matsuo, Takahiro Hata, Mutsuyoshi Matsushita
Conventional clinical treatments for allergy management remain suboptimal; new, orally available medications that improve a wide range of allergic signs have been desired. We previously demonstrated that JTE-852, a novel spleen tyrosine kinase inhibitor, potently and simultaneously suppresses secretion of granule contents, arachidonate metabolites, and cytokines from mast cells stimulated by immunoglobulin E-crosslinking. In the present study, we investigated the effects of JTE-852 in four rat models (sneezing, rhinorrhea, airway constriction, and airway inflammation) as representatives of allergy models...
March 24, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29336005/degranulation-inhibitors-from-the-arils-of-myristica-fragrans-in-antigen-stimulated-rat-basophilic-leukemia-cells
#7
Toshio Morikawa, Ikuko Hachiman, Kiyofumi Ninomiya, Hiroki Hata, Kaoru Sugawara, Osamu Muraoka, Hisashi Matsuda
A methanol extract of mace, the aril of Myristica fragrans (Myristicaceae), was found to inhibit the release of β-hexosaminidase, a marker of antigen-IgE-stimulated degranulation in rat basophilic leukemia cells (RBL-2H3, IC50  = 45.7 μg/ml). From the extract, three new 8-O-4' type neolignans, maceneolignans I-K (1-3), were isolated, and the stereostructures of 1-3 were elucidated based on spectroscopic and chemical evidence. Among the isolates, maceneolignans A (5), D (6), and H (8), (-)-(8R)-∆8' -4-hydroxy-3,3',5'-trimethoxy-8-O-4'-neolignan (13), (-)-(8R)-∆8' -3,4,5,3',5'-pentamethoxy-8-O-4'-neolignan (14), (-)-erythro-(7R,8S)-∆8' -7-acetoxy-3,4-methylenedioxy-3',5'-dimethoxy-8-O-4'-neolignan (17), (+)-licarin A (20), nectandrin B (24), verrucosin (25), and malabaricone C (29) were investigated as possible degranulation inhibitors (IC50  = 20...
March 2018: Journal of Natural Medicines
https://www.readbyqxmd.com/read/29244610/inhibition-of-exosome-release-by-ketotifen-enhances-sensitivity-of-cancer-cells-to-doxorubicin
#8
Farman Matloob Khan, Ekram Saleh, Hussain Alawadhi, Rania Harati, Wolfram-Hubertus Zimmermann, Raafat El-Awady
Exosomes released from cancer cells support metastasis and growth of recipient cells and increase their resistance to chemotherapy. Therapeutic targeting of exosomes is a promising area in cancer research. Our aim is to test the effect of the mast cell stabilizer ketotifen on exosomes release from cancer cells and how this can modify their response to doxorubicin. Exosomes release from three cancer cell lines (MCF7, HeLa and BT549) was assessed by scan electron microscope and exosomes quantification kit. Doxorubicin export within exosomes was monitored flurometrically and cellular sensitivity to doxorubicin ± ketotifen was measured by sulphorhodamine-B and colony formation assays...
January 2, 2018: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/29200930/survey-on-synergism-effect-of-ketotifen-in-combination-with-pyrimethamine-in-treatment-of-acute-murine-toxoplasmosis
#9
Mahbobeh Montazeri, Kian Rezaei, Mohammad Ali Ebrahimzadeh, Mehdi Sharif, Shahabeddin Sarvi, Ehsan Ahmadpour, Mohammad Taghi Rahimi, Abdol Satar Pagheh, Saeed Mehrzadi, Ahmad Daryani
Background: Standard treatment of toxoplasmosis is accompanied by severe side effects and low tolerability; accordingly, alternative medicines are critically needed. Ketotifen (KET) as a cell membrane stabilizer could be an appropriate inhibitor of Toxoplasma gondii ( T. gondii ) parasite entrance into the host cells. Therefore, the focus of current study is characterization of the anti- Toxoplasma activity of KET in the acute phase of toxoplasmosis in murine model as pre-treatment and post-treatment (before and after infection with RH strain)...
2017: Tropical Medicine and Health
https://www.readbyqxmd.com/read/29195028/effect-of-varicocelectomy-and-or-mast-cells-stabilizer-on-sperm-dna-fragmentation-in-infertile-patients-with-varicocele
#10
A Zaazaa, A Adel, I Fahmy, Y Elkhiat, A A Awaad, T Mostafa
This study aimed to assess the effect of varicocelectomy and/or mast cells (MCs) stabilizer on sperm DNA fragmentation in infertile men with varicocele (Vx). Overall, 120 infertile patients were randomized to three equal treatment arms; patients that underwent varicocelectomy, patients on 1 mg ketotifen twice daily for three months, and patients that underwent varicocelectomy followed with 1 mg ketotifen twice daily for three months. These patients were subjected to history taking, clinical examination, semen analysis, and estimation of sperm DNA fragmentation index (DFI)...
January 2018: Andrology
https://www.readbyqxmd.com/read/29129665/intravasation-of-sw620-colon-cancer-cell-spheroids-through-the-blood-endothelial-barrier-is-inhibited-by-clinical-drugs-and-flavonoids-in-vitro
#11
Silvio Holzner, Stefan Brenner, Atanas Georgiev Atanasov, Daniel Senfter, Serena Stadler, Chi Huu Nguyen, Adryan Fristiohady, Daniela Milovanovic, Nicole Huttary, Sigurd Krieger, Zsuzsanna Bago-Horvath, Oliver de Wever, Ioannis Tentes, Ali Özmen, Walter Jäger, Helmut Dolznig, Verena Maria Dirsch, Robert Michael Mader, Liselotte Krenn, Georg Krupitza
Mechanisms how colorectal cancer (CRC) cells penetrate blood micro-vessel endothelia and metastasise is poorly understood. To study blood endothelial cell (BEC) barrier breaching by CRC emboli, an in vitro assay measuring BEC-free areas underneath SW620 cell spheroids, so called "circular chemorepellent induced defects" (CCIDs, appearing in consequence of endothelial retraction), was adapted and supported by Western blotting, EIA-, EROD- and luciferase reporter assays. Inhibition of ALOX12 or NF-κB in SW620 cells or BECs, respectively, caused attenuation of CCIDs...
January 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29098871/design-of-antihistaminic-transdermal-films-based-on-alginate-chitosan-polyelectrolyte-complexes-characterization-and-permeation-studies
#12
Sonia Lefnaoui, Nadji Moulai-Mostefa, Madiha M Yahoum, Sarah N Gasmi
The purpose of this study was to develop suitable matrix-type transdermal drug delivery systems of Ketotifen fumarate (KF) as antiasthmatic drugs. Chitosan-alginate polyelectrolyte complex (PEC) films were used as drug release regulators for KF. Antihistaminic films with variable PEC compositions were prepared using different ratios of chitosan (CTS) to sodium alginate (ALG). Propylene glycol (PG) was used as plasticizer; Tween 80 (T80 ) and Span 20 (S20 ) were used as permeability enhancers. Nine formulations were obtained by film casting method and characterized in terms of weight uniformity, thickness, folding endurance, moisture lost, and moisture absorption...
March 2018: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28894590/trpa1-channel-mediates-organophosphate-induced-delayed-neuropathy
#13
Qiang Ding, Sui Fang, Xueqin Chen, Youxin Wang, Jian Li, Fuyun Tian, Xiang Xu, Bernard Attali, Xin Xie, Zhaobing Gao
The organophosphate-induced delayed neuropathy (OPIDN), often leads to paresthesias, ataxia and paralysis, occurs in the late-stage of acute poisoning or after repeated exposures to organophosphate (OP) insecticides or nerve agents, and may contribute to the Gulf War Syndrome. The acute phase of OP poisoning is often attributed to acetylcholinesterase inhibition. However, the underlying mechanism for the delayed neuropathy remains unknown and no treatment is available. Here we demonstrate that TRPA1 channel (Transient receptor potential cation channel, member A1) mediates OPIDN...
2017: Cell Discovery
https://www.readbyqxmd.com/read/28890861/idiopathic-anaphylaxis
#14
REVIEW
Bright I Nwaru, Sangeeta Dhami, Aziz Sheikh
Idiopathic anaphylaxis is a rare life-threatening disorder with symptoms similar to other forms of anaphylaxis. There is lack of a robust evidence base underpinning the treatment of anaphylaxis and even less so for idiopathic anaphylaxis. Much of the evidence therefore comes from relatively small case series and expert opinion. Idiopathic anaphylaxis is a diagnosis of exclusion, requiring a thorough history and careful diagnostic work-up investigating possible triggers and underlying predisposing factors. Key diagnostic tests include skin-prick testing, tests for specific-IgE, component-resolved diagnostics, and in some cases for allergen challenge tests...
2017: Current Treatment Options in Allergy
https://www.readbyqxmd.com/read/28840310/topical-cis-urocanic-acid-prevents-ocular-surface-irritation-in-both-ige-independent-and-mediated-rat-model
#15
Hanna-Mari Jauhonen, Jarmo Laihia, Olli Oksala, Johanna Viiri, Reijo Sironen, Päivi Alajuuma, Kai Kaarniranta, Lasse Leino
PURPOSE: Our purpose was to investigate the effect of locally administered cis-urocanic (cis-UCA) in two experimental models of allergic conjunctivitis. METHODS: The compound 48/80 (C48/80)-induced ocular irritation model (IgE-independent) and the ovalbumin (OA)-induced ocular allergy model (IgE-mediated) were used to test and compare the effect of cis-UCA on dexamethasone, ketotifen and olopatadine. In the C48/80 model, clinical severity scoring from photographs, immunohistochemical analysis of nuclear Ki-67 antigen to quantify actively proliferating epithelial cells and of caspase-3 enzyme to identify apoptotic activity in the conjunctival tissue were used...
December 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28811247/pharmacological-analysis-of-poecilotheria-spider-venoms-in-mice-provides-clues-for-human-treatment
#16
Alexander Andreev-Andrievskiy, Anfisa Popova, Evgeniia Lagereva, Daniil Osipov, Antonina Berkut, Eugene Grishin, Alexander Vassilevski
Bites of tiger spiders belonging to Poecilotheria genus cause moderate to severe pain and long-lasting local or generalized muscle cramps in humans. Bites occur in regions of the spiders' natural habitat, India and Sri Lanka, but the popularity of these colorful tarantulas as pets leads to reports of envenomation cases worldwide. Treatment is predominantly symptomatic and often inadequate since there is almost no clinical or toxicology research data available, and physicians outside India or Sri Lanka typically have no experience in treating such cases...
November 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28711627/vasoactive-intestinal-polypeptide-and-mast-cells-regulate-increased-passage-of-colonic-bacteria-in-patients-with-irritable-bowel-syndrome
#17
Olga Bednarska, Susanna A Walter, Maite Casado-Bedmar, Magnus Ström, Eloísa Salvo-Romero, Maria Vicario, Emeran A Mayer, Åsa V Keita
BACKGROUND & AIMS: Irritable bowel syndrome (IBS) is associated with intestinal dysbiosis and symptoms of IBS develop following gastroenteritis. We aimed to study the passage of live bacteria through the colonic epithelium, and determine the role of mast cells (MCs) and vasoactive intestinal polypeptide (VIP) in barrier regulation in IBS and healthy individuals. METHODS: Colon biopsies from 32 women with IBS and 15 age-matched healthy women (controls) were mounted in Ussing chambers; we measured numbers of fluorescently labeled Escherichia coli HS and Salmonella typhimurium that passed through from the mucosal side to the serosal side of the tissue...
October 2017: Gastroenterology
https://www.readbyqxmd.com/read/28659489/transcriptional-profiling-confirms-the-therapeutic-effects-of-mast-cell-stabilization-in-a-dengue-disease-model
#18
Juliet Morrison, Abhay P S Rathore, Chinmay K Mantri, Siti A B Aman, Andrew Nishida, Ashley L St John
There are no approved therapeutics for the treatment of dengue disease despite the global prevalence of dengue virus (DENV) and its mosquito vectors. DENV infections can lead to vascular complications, hemorrhage, and shock due to the ability of DENV to infect a variety of immune and nonimmune cell populations. Increasingly, studies have implicated the host response as a major contributor to severe disease. Inflammatory products of various cell types, including responding T cells, mast cells (MCs), and infected monocytes, can contribute to immune pathology...
September 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28584400/compound-48-80-a-mast-cell-degranulator-causes-oxidative-damage-by-enhancing-vitamin-c-synthesis-via-reduced-glutathione-depletion-and-lipid-peroxidation-through-neutrophil-infiltration-in-rat-livers
#19
Yosihiji Ohta, Koji Yashiro, Koji Ohashi, Yosuke Horikoshi, Chiaki Kusumoto, Tatsuya Matsura
In this study, we examined whether compound 48/80 (C48/80), a mast cell degranulator, causes hepatic oxidative damage in rats. Serum and liver biochemical parameters were determined 0.5, 3 or 6 h after a single treatment with C48/80 (0.75 mg/kg). Serum histamine and serotonin levels increased 0.5 h after C48/80 treatment but diminished thereafter. Increases in serum vitamin C (VC) and transaminases and hepatic hydrogen peroxide, lipid peroxide, and myeloperoxidase levels and a decrease in hepatic reduced glutathione level occurred 0...
May 2017: Journal of Clinical Biochemistry and Nutrition
https://www.readbyqxmd.com/read/28496459/fabrication-and-in-vitro-evaluation-of-ketotifen-fumarate-loaded-plga-nanoparticles-as-a-sustained-delivery-system
#20
Saieede Soltani, Parvin Zakeri-Milani, Mohammad Barzegar-Jalali, Mitra Jelvehgari
Ketotifen fumarate is a non-bronchodilator anti-asthmatic drug which inhibits the effects of certain endogenous substances known to be inflammatory mediators, and thereby exerts antiallergic activity. The present study describes the formulation of a sustained release nanoparticle (NP) drug delivery system containing ketoftifen, using poly (D,L lactide-co-glycolide) acid (PLGA). Biodegradable NPs were prepared using 50 : 50 PLGA by a water in-oil-in-water (w/o/w) double emulsion-solvent evaporation procedure and characterized for drug content, DSC (differential scanning calorimetry, XRD (X-ray diffractionl), FTIR (Fourier transform spectroscopy), particle size , surface morphology using scanning electron microscopy, and drug release rate...
2017: Iranian Journal of Pharmaceutical Research: IJPR
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