Read by QxMD icon Read

Robert waterland

Peter Kühnen, Daniela Handke, Robert A Waterland, Branwen J Hennig, Matt Silver, Anthony J Fulford, Paula Dominguez-Salas, Sophie E Moore, Andrew M Prentice, Joachim Spranger, Anke Hinney, Johannes Hebebrand, Frank L Heppner, Lena Walzer, Carsten Grötzinger, Jörg Gromoll, Susanna Wiegand, Annette Grüters, Heiko Krude
The estimated heritability of human BMI is close to 75%, but identified genetic variants explain only a small fraction of interindividual body-weight variation. Inherited epigenetic variants identified in mouse models named "metastable epialleles" could in principle explain this "missing heritability." We provide evidence that methylation in a variably methylated region (VMR) in the pro-opiomelanocortin gene (POMC), particularly in postmortem human laser-microdissected melanocyte-stimulating hormone (MSH)-positive neurons, is strongly associated with individual BMI...
September 13, 2016: Cell Metabolism
Elizabeth F Sutton, L Anne Gilmore, David B Dunger, Bas T Heijmans, Marie-France Hivert, Charlotte Ling, J Alfredo Martinez, Susan E Ozanne, Rebecca A Simmons, Moshe Szyf, Robert A Waterland, Leanne M Redman, Eric Ravussin
OBJECTIVE: On December 8-9, 2014, the Pennington Biomedical Research Center convened a scientific symposium to review the state-of-the-science and future directions for the study of developmental programming of obesity and chronic disease. The objectives of the symposium were to discuss: (i) past and current scientific advances in animal models, population-based cohort studies, and human clinical trials, (ii) the state-of-the-science of epigenetic-based research, and (iii) considerations for future studies...
May 2016: Obesity
Jesse D Eclarinal, Shaoyu Zhu, Maria S Baker, Danthasinghe B Piyarathna, Cristian Coarfa, Marta L Fiorotto, Robert A Waterland
Previous rodent studies have shown that maternal voluntary exercise during pregnancy leads to metabolic changes in adult offspring. We set out to test whether maternal voluntary exercise during pregnancy also induces persistent changes in voluntary physical activity in the offspring. Adult C57BL/6J female mice were randomly assigned to be caged with an unlocked (U) or locked (L) running wheel before and during pregnancy. Maternal running behavior was monitored during pregnancy, and body weight, body composition, food intake, energy expenditure, total cage activity, and running wheel activity were measured in the offspring at various ages...
July 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Robert Waterland
Transient nutritional exposures during critical ontogenic periods can cause persistent changes in gene expression, metabolism, and risk of various diseases. We have been investigating whether such 'developmental programming' occurs via nutritional influences on developmental epigenetics. Our studies in agouti viable yellow and axin-fused mice showed that developmental establishment of DNA methylation at 'metastable epialleles' is especially sensitive to maternal nutritional status around the time of conception...
2015: Journal of Nutritional Science and Vitaminology
Shaoyu Zhu, Jesse Eclarinal, Maria S Baker, Ge Li, Robert A Waterland
Extensive human and animal model data show that environmental influences during critical periods of prenatal and early postnatal development can cause persistent alterations in energy balance regulation. Although a potentially important factor in the worldwide obesity epidemic, the fundamental mechanisms underlying such developmental programming of energy balance are poorly understood, limiting our ability to intervene. Most studies of developmental programming of energy balance have focused on persistent alterations in the regulation of energy intake; energy expenditure has been relatively underemphasised...
October 29, 2015: Proceedings of the Nutrition Society
Da-Hai Yu, Manasi Gadkari, Quan Zhou, Shiyan Yu, Nan Gao, Yongtao Guan, Deborah Schady, Tony N Roshan, Miao-Hsueh Chen, Eleonora Laritsky, Zhongqi Ge, Hui Wang, Rui Chen, Caroline Westwater, Lynn Bry, Robert A Waterland, Chelsea Moriarty, Cindy Hwang, Alton G Swennes, Sean R Moore, Lanlan Shen
BACKGROUND: DNA methylation is an epigenetic mechanism central to development and maintenance of complex mammalian tissues, but our understanding of its role in intestinal development is limited. RESULTS: We use whole genome bisulfite sequencing, and find that differentiation of mouse colonic intestinal stem cells to intestinal epithelium is not associated with major changes in DNA methylation. However, we detect extensive dynamic epigenetic changes in intestinal stem cells and their progeny during the suckling period, suggesting postnatal epigenetic development in this stem cell population...
September 30, 2015: Genome Biology
Matt J Silver, Noah J Kessler, Branwen J Hennig, Paula Dominguez-Salas, Eleonora Laritsky, Maria S Baker, Cristian Coarfa, Hector Hernandez-Vargas, Jovita M Castelino, Michael N Routledge, Yun Yun Gong, Zdenko Herceg, Yong Sun Lee, Kwanbok Lee, Sophie E Moore, Anthony J Fulford, Andrew M Prentice, Robert A Waterland
BACKGROUND: Interindividual epigenetic variation that occurs systemically must be established prior to gastrulation in the very early embryo and, because it is systemic, can be assessed in easily biopsiable tissues. We employ two independent genome-wide approaches to search for such variants. RESULTS: First, we screen for metastable epialleles by performing genomewide bisulfite sequencing in peripheral blood lymphocyte (PBL) and hair follicle DNA from two Caucasian adults...
2015: Genome Biology
Mark L Wahlqvist, Stephen A Krawetz, Nico S Rizzo, Maria Gloria Dominguez-Bello, Linda M Szymanski, Shari Barkin, Ann Yatkine, Robert A Waterland, Julie A Mennella, Mina Desai, Michael G Ross, Nancy F Krebs, Bridget E Young, Jane Wardle, Christiane D Wrann, John G Kral
The double burden of under- and overnutrition profoundly affects human health globally. According to the World Health Organization, obesity and diabetes rates have almost doubled worldwide since 1980, and, in 2011, more than 40 million children under 5 years of age were overweight. Ecologic factors, parental genetics and fitness, and the intrauterine environment significantly influence the likelihood of offspring developing the dysmetabolic diathesis of obesity. This report examines the effects of these factors, including preconception, intrauterine and postnatal energy balance affecting programming of transgenerational transmission, and development of chronic diseases later in life-in particular, diabesity and its comorbidities...
July 2015: Annals of the New York Academy of Sciences
Anshul Kundaje, Wouter Meuleman, Jason Ernst, Misha Bilenky, Angela Yen, Alireza Heravi-Moussavi, Pouya Kheradpour, Zhizhuo Zhang, Jianrong Wang, Michael J Ziller, Viren Amin, John W Whitaker, Matthew D Schultz, Lucas D Ward, Abhishek Sarkar, Gerald Quon, Richard S Sandstrom, Matthew L Eaton, Yi-Chieh Wu, Andreas R Pfenning, Xinchen Wang, Melina Claussnitzer, Yaping Liu, Cristian Coarfa, R Alan Harris, Noam Shoresh, Charles B Epstein, Elizabeta Gjoneska, Danny Leung, Wei Xie, R David Hawkins, Ryan Lister, Chibo Hong, Philippe Gascard, Andrew J Mungall, Richard Moore, Eric Chuah, Angela Tam, Theresa K Canfield, R Scott Hansen, Rajinder Kaul, Peter J Sabo, Mukul S Bansal, Annaick Carles, Jesse R Dixon, Kai-How Farh, Soheil Feizi, Rosa Karlic, Ah-Ram Kim, Ashwinikumar Kulkarni, Daofeng Li, Rebecca Lowdon, GiNell Elliott, Tim R Mercer, Shane J Neph, Vitor Onuchic, Paz Polak, Nisha Rajagopal, Pradipta Ray, Richard C Sallari, Kyle T Siebenthall, Nicholas A Sinnott-Armstrong, Michael Stevens, Robert E Thurman, Jie Wu, Bo Zhang, Xin Zhou, Arthur E Beaudet, Laurie A Boyer, Philip L De Jager, Peggy J Farnham, Susan J Fisher, David Haussler, Steven J M Jones, Wei Li, Marco A Marra, Michael T McManus, Shamil Sunyaev, James A Thomson, Thea D Tlsty, Li-Huei Tsai, Wei Wang, Robert A Waterland, Michael Q Zhang, Lisa H Chadwick, Bradley E Bernstein, Joseph F Costello, Joseph R Ecker, Martin Hirst, Alexander Meissner, Aleksandar Milosavljevic, Bing Ren, John A Stamatoyannopoulos, Ting Wang, Manolis Kellis
The reference human genome sequence set the stage for studies of genetic variation and its association with human disease, but epigenomic studies lack a similar reference. To address this need, the NIH Roadmap Epigenomics Consortium generated the largest collection so far of human epigenomes for primary cells and tissues. Here we describe the integrative analysis of 111 reference human epigenomes generated as part of the programme, profiled for histone modification patterns, DNA accessibility, DNA methylation and RNA expression...
February 19, 2015: Nature
Mark H Russell, Robert L Waterland, Fiona Wong
Determination of the chemical clearance rate from human blood is a critical component of toxicokinetic exposure assessment. Analysis of temporal biomonitoring data without consideration of ongoing exposure results in calculation of apparent elimination half-life values that are longer than the intrinsic value. The intrinsic elimination half-life is solely a function of the rate of elimination while the apparent elimination half-life reflects the processes of both elimination and ongoing exposure. Confusion between intrinsic and apparent half-life values can lead to misinterpretation of biomonitoring data and can result in exaggerated predictions in subsequent modeling efforts...
June 2015: Chemosphere
Da-Hai Yu, Robert A Waterland, Pumin Zhang, Deborah Schady, Miao-Hsueh Chen, Yongtao Guan, Manasi Gadkari, Lanlan Shen
Cancer has long been viewed as a genetic disease; however, epigenetic silencing as the result of aberrant promoter DNA methylation is frequently associated with cancer development, suggesting an epigenetic component to the disease. Nonetheless, it has remained unclear whether an epimutation (an aberrant change in epigenetic regulation) can induce tumorigenesis. Here, we exploited a functionally validated cis-acting regulatory element and devised a strategy to induce developmentally regulated genomic targeting of DNA methylation...
September 2014: Journal of Clinical Investigation
Robert A Waterland
Extensive human and animal model data show that nutrition and other environmental influences during critical periods of embryonic, fetal, and early postnatal life can affect the development of body weight regulatory pathways, with permanent consequences for risk of obesity. Epigenetic processes are widely viewed as a leading mechanism to explain the lifelong persistence of such "developmental programming" of energy balance. Despite meaningful progress in recent years, however, significant research obstacles impede our ability to test this hypothesis...
2014: Annual Review of Nutrition
Paula Dominguez-Salas, Sophie E Moore, Maria S Baker, Andrew W Bergen, Sharon E Cox, Roger A Dyer, Anthony J Fulford, Yongtao Guan, Eleonora Laritsky, Matt J Silver, Gary E Swan, Steven H Zeisel, Sheila M Innis, Robert A Waterland, Andrew M Prentice, Branwen J Hennig
In experimental animals, maternal diet during the periconceptional period influences the establishment of DNA methylation at metastable epialleles in the offspring, with permanent phenotypic consequences. Pronounced naturally occurring seasonal differences in the diet of rural Gambian women allowed us to test this in humans. We show that significant seasonal variations in methyl-donor nutrient intake of mothers around the time of conception influence 13 relevant plasma biomarkers. The level of several of these maternal biomarkers predicts increased/decreased methylation at metastable epialleles in DNA extracted from lymphocytes and hair follicles in infants postnatally...
2014: Nature Communications
Aaron M Jubb, Tomasz Gierczak, Munkhbayar Baasandorj, Robert L Waterland, James B Burkholder
Mixtures of methyl-perfluoroheptene-ethers (CH3OC7F13, MPHEs) are currently in use as replacements for perfluorinated alkanes (PFCs) and poly-ether heat transfer fluids, which are persistent greenhouse gases with lifetimes >1000 years. At present, the atmospheric processing and environmental impact from the use of MPHEs is unknown. In this work, rate coefficients at 296 K for the gas-phase reaction of the OH radical with six key isomers (including stereoisomers and enantiomers) of MPHEs used commercially were measured using a relative rate method...
May 6, 2014: Environmental Science & Technology
Steven B Heymsfield, Nicole M Avena, Leslie Baier, Phillip Brantley, George A Bray, Lisa C Burnett, Merlin G Butler, Daniel J Driscoll, Dieter Egli, Joel Elmquist, Janice L Forster, Anthony P Goldstone, Linda M Gourash, Frank L Greenway, Joan C Han, James G Kane, Rudolph L Leibel, Ruth J F Loos, Ann O Scheimann, Christian L Roth, Randy J Seeley, Val Sheffield, Maïthé Tauber, Christian Vaisse, Liheng Wang, Robert A Waterland, Rachel Wevrick, Jack A Yanovski, Andrew R Zinn
OBJECTIVE: Hyperphagia is a central feature of inherited disorders (e.g., Prader-Willi Syndrome) in which obesity is a primary phenotypic component. Hyperphagia may also contribute to obesity as observed in the general population, thus raising the potential importance of common underlying mechanisms and treatments. Substantial gaps in understanding the molecular basis of inherited hyperphagia syndromes are present as are a lack of mechanistic of mechanistic targets that can serve as a basis for pharmacologic and behavioral treatments...
February 2014: Obesity
Govindarajan Kunde-Ramamoorthy, Cristian Coarfa, Eleonora Laritsky, Noah J Kessler, R Alan Harris, Mingchu Xu, Rui Chen, Lanlan Shen, Aleksandar Milosavljevic, Robert A Waterland
Coupling bisulfite conversion with next-generation sequencing (Bisulfite-seq) enables genome-wide measurement of DNA methylation, but poses unique challenges for mapping. However, despite a proliferation of Bisulfite-seq mapping tools, no systematic comparison of their genomic coverage and quantitative accuracy has been reported. We sequenced bisulfite-converted DNA from two tissues from each of two healthy human adults and systematically compared five widely used Bisulfite-seq mapping algorithms: Bismark, BSMAP, Pash, BatMeth and BS Seeker...
April 2014: Nucleic Acids Research
Ge Li, Wenjuan Zhang, Maria S Baker, Eleonora Laritsky, Natalia Mattan-Hung, Dahai Yu, Govindarajan Kunde-Ramamoorthy, Richard B Simerly, Rui Chen, Lanlan Shen, Robert A Waterland
Prenatal and early postnatal environment can persistently alter one's risk of obesity. Environmental effects on hypothalamic developmental epigenetics constitute a likely mechanism underlying such 'developmental programming' of energy balance regulation. To advance our understanding of these processes, it is essential to develop approaches to disentangle the cellular and regional heterogeneity of hypothalamic developmental epigenetics. We therefore performed genome-scale DNA methylation profiling in hypothalamic neurons and non-neuronal cells at postnatal day 0 (P0) and P21 and found, surprisingly, that most of the DNA methylation differences distinguishing these two cell types are established postnatally...
March 15, 2014: Human Molecular Genetics
Paula Dominguez-Salas, Sophie E Moore, Darren Cole, Kerry-Ann da Costa, Sharon E Cox, Roger A Dyer, Anthony J C Fulford, Sheila M Innis, Robert A Waterland, Steven H Zeisel, Andrew M Prentice, Branwen J Hennig
BACKGROUND: Animal models show that periconceptional supplementation with folic acid, vitamin B-12, choline, and betaine can induce differences in offspring phenotype mediated by epigenetic changes in DNA. In humans, altered DNA methylation patterns have been observed in offspring whose mothers were exposed to famine or who conceived in the Gambian rainy season. OBJECTIVE: The objective was to understand the seasonality of DNA methylation patterns in rural Gambian women...
June 2013: American Journal of Clinical Nutrition
Ge Li, John J Kohorst, Wenjuan Zhang, Eleonora Laritsky, Govindarajan Kunde-Ramamoorthy, Maria S Baker, Marta L Fiorotto, Robert A Waterland
Decades of research in rodent models has shown that early postnatal overnutrition induces excess adiposity and other components of metabolic syndrome that persist into adulthood. The specific biologic mechanisms explaining the persistence of these effects, however, remain unknown. On postnatal day 1 (P1), mice were fostered in control (C) or small litters (SL). SL mice had increased body weight and adiposity at weaning (P21), which persisted to adulthood (P180). Detailed metabolic studies indicated that female adult SL mice have decreased physical activity and energy expenditure but not increased food intake...
August 2013: Diabetes
Da-Hai Yu, Carol Ware, Robert A Waterland, Jiexin Zhang, Miao-Hsueh Chen, Manasi Gadkari, Govindarajan Kunde-Ramamoorthy, Lagina M Nosavanh, Lanlan Shen
During development, a small but significant number of CpG islands (CGIs) become methylated. The timing of developmentally programmed CGI methylation and associated mechanisms of transcriptional regulation during cellular differentiation, however, remain poorly characterized. Here, we used genome-wide DNA methylation microarrays to identify epigenetic changes during human embryonic stem cell (hESC) differentiation. We discovered a group of CGIs associated with developmental genes that gain methylation after hESCs differentiate...
May 2013: Molecular and Cellular Biology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"