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https://www.readbyqxmd.com/read/29350361/evaluation-of-tourniquet-application-in-a-simulated-tactical-environment
#1
Tomasz Sanak, Robert Brzozowski, Marek Dabrowski, Magdalena Kozak, Agata Dabrowska, Maciej Sip, Katarzyna Naylor, Kamil Torres
BACKGROUND: Application of a tourniquet in a tactical environment is implemented in two ways: the so-called self-aid, which is the application of a tourniquet by the injured, and the so-called buddy aid, which is the application of a tourniquet by the person provide aid. This study aimed to test the quality of tourniquet use in a simulated situation, close quarter battle. METHODS: The study involved 24 injured operators and 72 operators in the whole simulation, implying 12 sections of six individuals...
January 2018: Ulusal Travma Ve Acil Cerrahi Dergisi, Turkish Journal of Trauma & Emergency Surgery: TJTES
https://www.readbyqxmd.com/read/29345115/metatranscriptome-analysis-reveals-environmental-and-diel-regulation-of-a-heterosigma-akashiwo-raphidophyceae-bloom
#2
Nanjing Ji, Lingxiao Lin, Ling Li, Liying Yu, Yaqun Zhang, Hao Luo, Meizhen Li, Xinguo Shi, Da-Zhi Wang, Senjie Lin
Despite numerous laboratory studies on physiologies of harmful algal bloom (HAB) species, physiologies of these algae during a natural bloom are understudied. Here, we investigated a bloom of the raphidophyte Heterosigma akashiwo in the East China Sea in 2014 using metabarcode (18S rDNA) and metatranscriptome sequencing. Based on 18S rDNA analyses, the phytoplankton community shifted from high diversity in the pre-bloom stage to H. akashiwo predominance during the bloom. A sharp decrease in ambient dissolved inorganic phosphate and strong up-regulation of phosphate and dissolved organic phosphorus (DOP) uptake genes, including the rarely documented (ppGpp)ase, in H...
January 18, 2018: Environmental Microbiology
https://www.readbyqxmd.com/read/29344457/development-and-implementation-of-module-for-medical-graduates-to-improve-socio-cultural-sensitivity-towards-people-living-with-hiv
#3
Sushanta Kumar Mishra, Somanath Dash
Background: Health professionals are documented as an important cause for stigmatizing people living with HIV (PLHIV). Since traditional teaching on HIV in India does not address cultural competencies, medical graduates lack sociocultural sensitiveness while addressing the health needs of PLHIV. Aim: The aim of this study is to develop and to implement a module for medical graduates to improve their sociocultural sensitivity toward PLHIV. Methodology: A module was designed and introduced to address the core sensitive issues in HIV among medical graduates with the help of trained faculty...
December 2017: International Journal of Applied and Basic Medical Research
https://www.readbyqxmd.com/read/29340866/-in-silico-study-of-the-binding-of-two-novel-antagonists-to-the-nociceptin-receptor
#4
Stefano Della Longa, Alessandro Arcovito
Antagonists of the nociceptin receptor (NOP) are raising interest for their possible clinical use as antidepressant drugs. Recently, the structure of NOP in complex with some piperidine-based antagonists has been revealed by X-ray crystallography. In this study, a multi-flexible docking (MF-docking) procedure, i.e. docking to multiple receptor conformations extracted by preliminary molecular dynamics trajectories, together with hybrid quantum mechanics/molecular mechanics (QM/MM) simulations have been carried out to provide the binding mode of two novel NOP antagonists, one of them selective (BTRX-246040, formerly named LY-2940094) and one non selective (AT-076), i...
January 16, 2018: Journal of Computer-aided Molecular Design
https://www.readbyqxmd.com/read/29336119/evaluations-of-ultraiq-software-for-objective-ultrasound-image-quality-assessment-using-images-from-a-commercial-scanner
#5
Zaiyang Long, Donald J Tradup, Scott F Stekel, Krzysztof R Gorny, Nicholas J Hangiandreou
We evaluated a commercially available software package that uses B-mode images to semi-automatically measure quantitative metrics of ultrasound image quality, such as contrast response, depth of penetration (DOP), and spatial resolution (lateral, axial, and elevational). Since measurement of elevational resolution is not a part of the software package, we achieved it by acquiring phantom images with transducers tilted at 45 degrees relative to the phantom. Each measurement was assessed in terms of measurement stability, sensitivity, repeatability, and semi-automated measurement success rate...
January 16, 2018: Journal of Applied Clinical Medical Physics
https://www.readbyqxmd.com/read/29286827/the-role-of-anxiety-in-simulation-based-dexterity-and-overall-performance-does-it-really-matter
#6
Fatema Dhaif, Georgios Paparoidamis, Michail Sideris, John Hanrahan, Efstratia-Maria Georgopoulou, Ismini Tsagkaraki, Nikolaos Staikoglou, Ferha Saeed, Theodoros Michail, Anastasios Tzavelas, Efstathios Kenanidis, Michael Potoupnis, Eleftherios Tsiridis, Apostolos Papalois
BACKGROUND: Essential Skills in the Management of Surgical Cases (ESMSC) is an international undergraduate surgical masterclass which combines ex vivo, dry lab and high fidelity in vivo simulation-based learning (SBL). It consists of 32 stations of skills-based learning, including open reduction internal fixation (ORIF) of fractures. Current literature suggests early involvement in skills-based learning at the undergraduate level is vital. AIMS: To compare students' dexterity and skills-based performance with demographic and educational background parameters...
December 29, 2017: Journal of Investigative Surgery: the Official Journal of the Academy of Surgical Research
https://www.readbyqxmd.com/read/29276753/indian-hedgehog-suppresses-a-stromal-cell-driven-intestinal-immune-response
#7
B Florien Westendorp, Nikè V J A Büller, Olga N Karpus, Willemijn A van Dop, Jan Koster, Rogier Versteeg, Pim J Koelink, Clinton Y Snel, Sander Meisner, Joris J T H Roelofs, Anja Uhmann, Emiel Ver Loren van Themaat, Jarom Heijmans, Heidi Hahn, Vanesa Muncan, Manon E Wildenberg, Gijs R van den Brink
Background & Aims: Upon intestinal epithelial damage a complex wound healing response is initiated to restore epithelial integrity and defend against pathogenic invasion. Epithelium-derived Indian Hedgehog (Ihh) functions as a critical sensor in this process. Signaling occurs in a paracrine manner because the receptor for Ihh is expressed only in the mesenchyme, but the exact Hedgehog target cell has remained elusive. The aim of this study was to elucidate further the nature of this target cell in the context of intestinal inflammation...
2018: Cellular and Molecular Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/29249978/prediction-of-scar-size-in-rats-six-months-after-burns-based-on-early-post-injury-polarization-sensitive-optical-frequency-domain-imaging
#8
Eli Kravez, Martin Villiger, Brett Bouma, Martin Yarmush, Zohar Yakhini, Alexander Golberg
Hypertrophic scars remain a major clinical problem in the rehabilitation of burn survivors and lead to physical, aesthetic, functional, psychological, and social stresses. Prediction of healing outcome and scar formation is critical for deciding on the best treatment plan. Both subjective and objective scales have been devised to assess scar severity. Whereas scales of the first type preclude cross-comparison between observers, those of the second type are based on imaging modalities that either lack the ability to image individual layers of the scar or only provide very limited fields of view...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29246711/validity-and-reliability-of-doppler-ultrasonography-and-direct-arterial-blood-pressure-measurements-in-anaesthetized-dogs-weighing-less-than-5-kg
#9
Xavier Moll, Adria Aguilar, Felix García, Rosa Ferrer, Anna Andaluz
OBJECTIVE: To assess the validity and reliability of Doppler ultrasonography (DOP) as compared with invasive arterial blood pressure measurements in anaesthetized dogs weighing less than 5 kg. STUDY DESIGN: Controlled, prospective, clinical study. ANIMALS: A total of 41 privately owned dogs weighing less than 5 kg. METHODS: The dogs were anaesthetized, and an intra-arterial catheter was placed aseptically in the dorsal pedal artery of the pelvic limb to perform invasive blood pressure (IBP) measurement...
September 15, 2017: Veterinary Anaesthesia and Analgesia
https://www.readbyqxmd.com/read/29243428/-relative-molecular-mass-and-monosaccharide-composition-of-polysaccharide-in-dendrobium-officinale-flowers
#10
Si-Jie Zhang, Zheng Qian, Jing-Jing Liu, Xin-Feng Zhang, Jin-Ping Si
In order to provide new information on polysaccharide in Dendrobium officinale flowers,the monosaccharide composition and relative molecular mass distribution of 11 families of flowers were investigated and analyzed by high performance gel filtration chromatography (HPGFC) and pre-column derivatization ultra performance liquid chromatography (UPLC) in this study. Then cluster analysis was carried out for the monosaccharide peak areas by utilizing SPSS 19.0 software. The results showed that the polysaccharides of all the above 11 hybrid families of D...
October 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29240996/transfer-mechanism-uptake-kinetic-process-and-bioavailability-of-p-cu-cd-pb-and-zn-in-macrophyte-rhizosphere-using-dgt
#11
Shengrui Wang, Zhihao Wu, Jun Luo
The transfer-uptake-bioavailability of phosphorus (P), Cu, Cd, Zn and Pb in rhizosphere of Zizania latifolia (ZL) and Myriophyllum verticiilaturn (MV) cultivated in rhizoboxes in Lake Erhai (China) is investigated by DGT (diffusive gradients in thin films) technique. DGT induced fluxes in sediments (DIFS) model reveals that resupply ability (r), liable pool size in sediment solid (kd), kinetic parameter (k-1) or response time (Tc) control the diffusion-resupply characters of P and Cu (standing for four metals) in rhizosphere interface...
December 14, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29236847/protocol-for-chromosome-specific-probe-construction-using-prins-micromanipulation-and-dop-pcr-techniques
#12
Paulo Z Passamani, Carlos R Carvalho, Fernanda A F Soares
Chromosome-specific probes have been widely used in molecular cytogenetics, being obtained with different methods. In this study, a reproducible protocol for construction of chromosome-specific probes is proposed which associates in situ amplification (PRINS), micromanipulation and degenerate oligonucleotide-primed PCR (DOP-PCR). Human lymphocyte cultures were used to obtain metaphases from male and female individuals. The chromosomes were amplified via PRINS, and subcentromeric fragments of the X chromosome were microdissected using microneedles coupled to a phase contrast microscope...
December 7, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/29233215/unraveling-amyloid-formation-paths-of-parkinson-s-disease-protein-%C3%AE-synuclein-triggered-by-anionic-vesicles
#13
Juris Kiskis, Istvan Horvath, Pernilla Wittung-Stafshede, Sandra Rocha
Amyloid formation of the synaptic brain protein α-synuclein (αS) is related to degeneration of dopaminergic neurons in Parkinson's disease patients. αS is thought to function in vesicle transport and fusion and it binds strongly to negatively charged vesicles in vitro. Here we combined circular dichroism, fluorescence and imaging methods in vitro to characterize the interaction of αS with negatively charged vesicles of DOPS (1,2-dioleoyl-sn-glycero-3-phospho-L-serine, sodium salt) and DOPG (1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol), sodium salt) and the consequences of such interactions on αS amyloid formation...
January 2017: Quarterly Reviews of Biophysics
https://www.readbyqxmd.com/read/29190559/environmental-effects-by-introducing-potamogeton-crispus-to-recover-a-eutrophic-lake
#14
Xiuyun Cao, Lingling Wan, Jian Xiao, Xiaoyan Chen, Yiyong Zhou, Zhicong Wang, Chunlei Song
Re-establishing submerged vegetation is considered an important tool to restore shallow eutrophic lakes. A whole year comparative field study was performed in a eutrophic lake and its connected pond with Potamogeton crispus in order to determine the effects of the growth and senescence of submerged macrophytes on structure of phytoplankton. P. crispus improved the water quality at the growing season in terms of improving transparency, decreasing total phosphorus, soluble reactive phosphorus (SRP) and chlorophyll a concentrations and slowering turnover rate of dissolved organic phosphorus (DOP)...
November 27, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29188739/factors-influencing-the-educational-impact-of-mini-cex-and-dops-a-qualitative-synthesis
#15
Andrea C Lörwald, Felicitas-Maria Lahner, Robert Greif, Christoph Berendonk, John Norcini, Sören Huwendiek
INTRODUCTION: The educational impact of Mini-CEX and DOPS varies greatly and can be influenced by several factors. However, there is no comprehensive analysis and synthesis of the described influencing factors. METHODS: To fill this gap, we chose a two-step approach. First, we performed a systematic literature review and selected articles describing influencing factors on the educational impact of Mini-CEX and DOPS. Second, we performed a qualitative synthesis of these factors...
November 30, 2017: Medical Teacher
https://www.readbyqxmd.com/read/29159727/determining-inorganic-and-organic-phosphorus
#16
Jaana Koistinen, Mervi Sjöblom, Kristian Spilling
Phosphorus (P) is a macronutrient for all microalgal species, and the main form of uptake is orthophosphate (PO4). In this chapter we present a colorimetric method for determining the PO4 concentration and dissolved organic phosphorus (DOP) based on total phosphorus (TP) measurements. We also describe a method for determining particulate organic phosphorus (POP) based on the same principles.
November 21, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29156432/the-effects-of-human-drugs-in-corbicula-fluminea-assessment-of-neurotoxicity-inflammation-gametogenic-activity-and-energy-status
#17
G V Aguirre-Martínez, C André, F Gagné, L M Martín-Díaz
The constant release of pharmaceuticals products to aquatic environment even at low concentrations (ng L(-1) to µg L(-1)) could lead to unknown chronic effects to non-target organisms. The aim of this study was to evaluate neurotoxic responses, inflammation, gametogenic activity and energy status on the fresh water clam C. fluminea after exposure to different concentrations of caffeine (CAF), ibuprofen (IBU), carbamazepine (CBZ), novobiocin (NOV) and tamoxifen (TMX) for 21 days under laboratory conditions...
November 17, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29155273/mu-opioid-peptide-mop-and-nociceptin-orphanin-fq-peptide-nop-receptor-activation-both-contribute-to-the-discriminative-stimulus-properties-of-cebranopadol-in-the-rat
#18
Thomas M Tzschentke, Kris Rutten
The novel potent analgesic cebranopadol is an agonist at nociceptin/orphanin FQ peptide (NOP) and classical opioid receptors, with the highest in-vitro activity at NOP and mu-opioid peptide (MOP) receptors, and somewhat lower activity at kappa-opioid peptide (KOP) and delta-opioid peptide (DOP) receptors. We addressed the question of which of these pharmacological activities contribute to the stimulus properties of cebranopadol using a rat drug discrimination procedure. First, cebranopadol was tested in generalization tests against a morphine cue, including receptor-specific antagonism...
November 16, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/29152051/%C3%AF-space-screening-of-dermorphin-based-tetrapeptides-through-use-of-constrained-arylazepinone-and-quinolinone-scaffolds
#19
Olivier Van der Poorten, Robin Van Den Hauwe, Emilie Eiselt, Cecilia Betti, Karel Guillemyn, Nga N Chung, François Hallé, Frédéric Bihel, Peter W Schiller, Dirk Tourwé, Philippe Sarret, Louis Gendron, Steven Ballet
Herein, the synthesis of novel conformationally constrained amino acids, 4-amino-8-bromo-2-benzazepin-3-one (8-Br-Aba), 3-amino-3,4-dihydroquinolin-2-one, and regioisomeric 4-amino-naphthoazepinones (1- and 2-Ana), is described. Introduction of these constricted scaffolds into the N-terminal tetrapeptide of dermorphin (i.e., H-Tyr-d-Ala-Phe-Gly-NH2) induced significant shifts in binding affinity, selectivity, and in vitro activity at the μ- and δ-opioid receptors (MOP and DOP, respectively). A reported constrained μ-/δ-opioid lead tetrapeptide H-Dmt-d-Arg-Aba-Gly-NH2 was modified through application of various constrained building blocks to identify optimal spatial orientations in view of activity at the opioid receptors...
November 9, 2017: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29131989/microwave-assisted-photocatalysis-of-neurotoxin-compounds-using-metal-oxides-quantum-dots-nanosheets-composites-photocorrosion-inhibition-reusability-and-antibacterial-activity-studies
#20
Ali Fakhri, Mona Azad, Leila Fatolahi, Shiva Tahami
Water pollution caused by different pollutants is one of the challenging tasks for the scientific community. We have prepared and characterized a material for removal of pollutant compounds. ZnO quantum dots decorated CuO nanosheets and TiO2 quantum dots decorated WO3 nanosheets composites have been prepared using a hydrothermal method. The as synthesized catalysts were characterized by various techniques. The crystallite sizes of CuO NSs and WO3 NSs were to be obtained 12.5 and 13.25nm and when dopped with ZnO and TiO2 size reduces to 3...
November 10, 2017: Journal of Photochemistry and Photobiology. B, Biology
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