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Platelet-rich fibrin

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https://www.readbyqxmd.com/read/28931468/platelet-released-growth-factors-inhibit-proliferation-of-primary-keratinocytes-in-vitro
#1
Andreas Bayer, Mersedeh Tohidnezhad, Rouven Berndt, Sebastian Lippross, Peter Behrendt, Tim Klüter, Thomas Pufe, Holger Jahr, Jochen Cremer, Franziska Rademacher, Maren Simanski, Regine Gläser, Jürgen Harder
Autologous thrombocyte concentrate lysates as platelet-released growth factors (PRGF) or Vivostat Platelet Rich Fibrin (PRF(®)) represent important tools in modern wound therapy, especially in the treatment of chronic, hard-to-heal or infected wounds. Nevertheless, underlying cellular and molecular mechanisms of the beneficial clinical effects of a local wound therapy with autologous thrombocyte concentrate lysates are poorly understood. Recently, we have demonstrated that PRGF induces antimicrobial peptides in primary keratinocytes and accelerates keratinocytes' differentiation...
September 17, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28926988/quality-assessment-of-platelet-rich-fibrin-like-matrix-prepared-from-whole-blood-samples-after-extended-storage
#2
Hideo Kawabata, Kazushige Isobe, Taisuke Watanabe, Toshimitsu Okudera, Masayuki Nakamura, Masashi Suzuki, Jietsu Ryu, Yutaka Kitamura, Hajime Okudera, Kazuhiro Okuda, Koh Nakata, Tomoyuki Kawase
The platelet-rich fibrin-like matrix (PRFM) is usually prepared onsite and immediately used for regenerative therapy. Nonetheless, to meet the clinical necessity of preserving the PRFM without quality deterioration, we developed a method for preparation of PRFMs from short-term-stored whole blood (WB) samples. In this study, to evaluate the practical expiration date of storage, we extended the storage time of WB samples from 2 to 7 days and assessed the quality of the resulting PRFMs. WB samples collected with acid-citrate-dextrose were stored with gentle agitation at ambient temperature...
September 18, 2017: Biomedicines
https://www.readbyqxmd.com/read/28914251/clinical-and-radiographic-comparison-of-platelet-rich-fibrin-and-mineral-trioxide-aggregate-as-pulpotomy-agents-in-primary-molars
#3
Surendra Patidar, Namita Kalra, Amit Khatri, Rishi Tyagi
AIM: This study aimed to evaluate and compare the Platelet-rich fibrin (PRF) and Mineral trioxide aggregate (MTA) as a pulpotomy agent in primary molars. MATERIAL AND METHODS: In this study, 50 primary molars from 50 healthy children aged 5-9 years requiring pulpotomy were randomly allocated into two groups. In PRF group, after coronal pulp removal and hemostasis, remaining pulp tissue was covered with PRF preparation. In the MTA group, the pulp stumps were covered with MTA (Pro Root MTA-Root Canal Repair Material, Dentsply International Inc...
October 2017: Journal of the Indian Society of Pedodontics and Preventive Dentistry
https://www.readbyqxmd.com/read/28893718/alternatives-to-connective-tissue-graft-in-the-treatment-of-localized-gingival-recessions-a-systematic-review
#4
Khadija Amine, Yasser El Amrani, Siham Chemlali, Jamila Kissa
AIM: The aim of this Systematic Review (SR) was to assess the clinical efficacy of alternatives procedures; Acellular Dermal Matrix (ADM), Xenogeneic Collagen Matrix (XCM), Enamel Matrix Derivative (EMD) and Platelet Rich Fibrin (PRF), compared to conventional procedures in the treatment of localized gingival recessions. MATERIAL AND METHODS: Electronic searches were performed to identify randomized clinical trials (RCTs) on treatment of single gingival recession with at least 6 months of follow-up...
September 8, 2017: Journal of Stomatology, Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28893049/comparative-evaluation-of-the-efficacy-of-bioactive-ceramic-composite-granules-alone-and-in-combination-with-platelet-rich-fibrin-in-the-treatment-of-mandibular-class-ii-furcation-defects-a-clinical-and-radiographic-study
#5
Hima S Lohi, Dilip G Nayak, Ashita S Uppoor
INTRODUCTION: Predictable closure of furcation defects with bone grafts, Guided Tissue Regeneration (GTR) and a combination of the two has remained an elusive goal so far. Hence, evaluation of biomimetic agents as candidate technologies for periodontal regeneration merit due consideration. In this study, Choukroun's Platelet Rich Fibrin (PRF), a second generation platelet concentrate, is combined with bone graft to examine if the addition enhances the therapeutic potential of bone graft in the management of Class II furcation defects...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28893033/comparative-evaluation-of-bioactive-glass-putty-and-platelet-rich-fibrin-in-the-treatment-of-human-periodontal-intrabony-defects-a-randomized-control-trial
#6
Akbar Naqvi, D Gopalakrishnan, Meenu Taneja Bhasin, Nilima Sharma, Khushtar Haider, Santosh Martande
INTRODUCTION: Platelet-Rich Fibrin (PRF) and bioactive glass putty have been shown to be effective in promoting reduction in probing depth, gain in clinical attachment, and defect fill in intrabony periodontal defects. The individual role played by bioactive glass putty in combination with PRF is yet to be elucidated. AIM: To compare the clinical effectiveness of the combination of PRF and bioactive glass putty and bioactive glass putty alone as regenerative techniques for intrabony defects in humans...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28892654/single-stage-cartilage-repair-using-platelet-rich-fibrin-scaffolds-with-autologous-cartilaginous-grafts
#7
Chin-Chean Wong, Chih-Hwa Chen, Wing P Chan, Li-Hsuan Chiu, Wei-Pin Ho, Fon-Jou Hsieh, You-Tzung Chen, Tsung-Lin Yang
BACKGROUND: To avoid complicated procedures requiring in vitro chondrocyte expansion for cartilage repair, the development of a culture-free, 1-stage approach combining platelet-rich fibrin (PRF) and autologous cartilage grafts may be the solution. PURPOSE: To develop a feasible 1-step procedure to combine PRF and autologous cartilage grafts for articular chondral defects. STUDY DESIGN: Controlled laboratory study Methods: The chemotactic effects of PRF on chondrocytes harvested from the primary culture of rabbit cartilage were evaluated in vitro and ex vivo...
September 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28887865/plasma-rich-plasma-the-ultimate-secret-for-youthful-skin-elixir-and-hair-growth-triggering
#8
REVIEW
Ebtisam Elghblawi
The clinical application of platelet-rich plasma (PRP) is based on the increase in the concentration of growth factors that are released from alpha-granule of the concentrated platelets and in the secretion of proteins which are able to capitalize on the healing process at the cellular level. It has been invented to restore the natural beauty by starting the natural rejuvenation process of the skin and aiming to make it function as a younger one and to keep the skin youthful and maintain it. Besides that, it is also emerged to include hairs as a new injectable procedure to enable stimulating hair growth locally and topically; preventing its fall; improving hair shaft, hair stem, and its caliber; increasing its shine, vitality, and pliability; and declining hair splitting and breakage...
September 8, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/28877148/treatment-of-a-chronic-cutaneous-surgical-wound-with-platelet-rich-fibrin
#9
Anthony J Chiaravalloti, Bella Zubkov, Alexander Zubkov
No abstract text is available yet for this article.
September 1, 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28862325/platelet-rich-fibrin-aspirin-complex-promotes-alveolar-bone-regeneration-in-periodontal-defect-in-rats
#10
J Du, S Mei, L Guo, Y Su, H Wang, Y Liu, Z Zhao, S Wang, Y Liu
BACKGROUND AND OBJECTIVES: The efficacy and outcomes of aspirin in local defects and the use of platelet-rich fibrin (PRF) in periodontal defects were investigated. Whether the PRF/aspirin complex is a suitable scaffold and delivery system to carry sustained-release aspirin/salicylic acid to promote periodontal bone regeneration was determined. MATERIAL AND METHODS: PRF and PRF/aspirin complex were prepared. The concentrations of aspirin/salicylic acid released from the PRF/aspirin complex were calculated at 37°C...
September 1, 2017: Journal of Periodontal Research
https://www.readbyqxmd.com/read/28860991/antithrombotic-potential-of-tormentil-extract-in-animal-models
#11
Natalia Marcinczyk, Dominika Jarmoc, Agnieszka Leszczynska, Agnieszka Zakrzeska, Karol Kramkowski, Jakub Strawa, Anna Gromotowicz-Poplawska, Ewa Chabielska, Michal Tomczyk
Potentilla species that have been investigated so far display pharmacological activity mainly due to the presence of polyphenols. Recently, it was shown that polyphenol-rich extract from rhizome of Potentilla erecta (tormentil extract) affects the metabolism of arachidonic acid and exerts both anti-inflammatory and anti-oxidant activities, suggesting a possible effect on thrombosis. Accordingly, the aim of the study was to evaluate the effect of tormentil extract on haemostasis in a rat model of thrombosis...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28859924/the-use-of-platelet-rich-fibrin-for-immediate-closure-of-acute-oroantral-communications-an-alternative-approach
#12
Kani Bilginaylar
Currently, the buccal advancement flap, palatal rotational flap, and buccal fat pad techniques are frequently used to close an oroantral communication (OAC). In this study, platelet-rich fibrin (PRF) clots were used for immediate closure of acute OACs after extraction of posterior maxillary molars. Acute oroantral perforations larger than 3 mm in diameter in 21 patients were treated uneventfully using PRF clots. None of the patients had systemic diseases or symptoms of sinus disease. After tooth extraction, the diagnosis of acute OAC was examined by the Valsalva maneuver (compressing the nostrils with the fingers and then blowing out air)...
August 3, 2017: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28857405/assessment-of-the-effectiveness-of-platelet-rich-fibrin-in-the-treatment-of-schneiderian-membrane-perforation
#13
Elif Öncü, Esin Kaymaz
BACKGROUND: The objective of this study is to evaluate the effect of Platelet rich fibrin (PRF) treatment of maxillary sinus membrane perforation on bone formation and new vascular supply and the success of dental implant survival rate. METHODS: The dataset for this retrospective study consists of patients who received sinus augmentation using the lateral wall technique. A total of 16 patients (20 sinuses) the patients without sinus membrane perforation (10 maxiller sinus area with sinus floor augmentation) and with Schneiderian perforation (10 maxiller sinus area repairing with PRF and augmented sinus floor area) were included in this study...
August 30, 2017: Clinical Implant Dentistry and related Research
https://www.readbyqxmd.com/read/28853913/comparison-of-the-effects-of-osteochondral-autograft-transplantation-with-platelet-rich-plasma-or-platelet-rich-fibrin-on-osteochondral-defects-in-a-rabbit-model
#14
Masahiro Maruyama, Hiroshi Satake, Tomoto Suzuki, Ryusuke Honma, Yasushi Naganuma, Yuya Takakubo, Michiaki Takagi
BACKGROUND: Although osteochondral autograft transplantation (OAT) provides satisfactory outcomes for osteochondral defects, for large defects OAT is often inadequate because of graft availability. Osteochondral allograft transplantation is an alternative treatment for large defects, but this approach is limited by graft storage constraints and carries disease transmission risks. Platelet-rich fibrin (PRF) is a second-generation platelet concentrate, and its positive effect on articular cartilage has been reported...
August 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28844306/signaling-molecules-and-pulp-regeneration
#15
Gottfried Schmalz, Matthias Widbiller, Kerstin M Galler
Signaling molecules play an essential role in tissue engineering because they regulate regenerative processes. Evidence exists from animal studies that single molecules such as members of the transforming growth factor beta superfamily and factors that induce the growth of blood vessels (vascular endothelial growth factor), nerves (brain-derived neurotrophic factor), or fibroblasts (fibroblast growth factor) may induce reparative dentin formation. Mainly the formation of atubular dentin (osteodentin) has been described after the application of single molecules or combinations of recombinant growth factors on healthy exposed pulps or in pulp regeneration...
September 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28839418/patient-centered-microsurgical-management-of-gingival-recession-using-coronally-advanced-flap-with-either-platelet-rich-fibrin-or-connective-tissue-graft-a-comparative-analysis
#16
Archana Kumar, Vivek Kumar Bains, Rajesh Jhingran, Ruchi Srivastava, Rohit Madan, Iram Rizvi
PURPOSE: To evaluate autologous platelet-rich fibrin (PRF) and autogenous connective tissue graft (CTG) in gingival recession defects in conjunction with coronally advanced flap (CAF) using a microsurgical technique. MATERIALS AND METHODS: Forty-five Class I and II recession defects were randomly equally (n = 15) divided into three groups: Group I sites treated with CAF with PRF, Group II sites treated with CAF with CTG, and Group III sites treated with CAF alone using microsurgical approach...
April 2017: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/28839404/comparative-evaluation-of-the-efficacy-of-platelet-rich-fibrin-and-calcium-phosphosilicate-putty-alone-and-in-combination-in-the-treatment-of-intrabony-defects-a-randomized-clinical-and-radiographic-study
#17
Isha Agrawal, Sarath Chandran, Priyadarshini Nadig
BACKGROUND: Combination of platelet-rich fibrin (PRF) and bone substitutes for the treatment of intrabony pockets is based on sound biologic rationale. The present study aimed to explore the clinical and radiographic effectiveness of autologous PRF and calcium phosphosilicate (CPS) putty alone and in combination in treatment of intrabony defects. MATERIALS AND METHODS: A total of 45 intrabony defects were selected and randomly divided into three groups. In Group I, mucoperiosteal flap elevation followed by placement of PRF was done...
April 2017: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/28827850/platelet-poor-plasma-as-a-supplement-for-fibroblasts-cultured-in-platelet-rich-fibrin
#18
Luiz Alexandre Chisini, Sarah Arangurem Karam, Thaís Gioda Noronha, Letícia Regina Morello Sartori, Alissa Schmidt San Martin, Flávio Fernando Demarco, Marcus Cristian Muniz Conde
The aim of this study was to evaluate the proliferation and adhesion of mesenchymal cells (3T3/NIH) in Dulbecco's Modified Eagle Medium(DMEM) supplemented with Platelet-Poor Plasma (PPP) in aPlatelet-Rich Fibrin (PRF) scaffold. Human blood was obtained and processed in a centrifuge considering the equation G=1.12xRx(RPM/1000)(2) to obtain PRF and PPP.Cell adhesion and maintenance analyses were performed by MTTassays in a 96 well plate withsupplemented DMEM: PPP (90:10) for 24 hours. Besides, the PRF was deposited in a 48 well plate and 10x10(4) cells were seeded above each PRF (n=3) with 800µl of DMEM: PPP (90:10) and cultured for 7 days...
June 2017: Acta Stomatologica Croatica
https://www.readbyqxmd.com/read/28827058/apprising-the-diverse-facets-of-platelet-rich-fibrin-in-surgery-through-a-systematic-review
#19
REVIEW
S Nanditha, Balamanikandasrinivasan Chandrasekaran, Senthilkumar Muthusamy, Kavitha Muthu
CONTEXT: Comprehensive reviews on clinical applications of Platelet rich fibrin (PRF) in surgery are limited despite its extensive utilization resulting in a dearth of knowledge on its effectiveness. Hence this article focuses on shedding light to the various applications of PRF pertaining to surgery through a systematic review. OBJECTIVE: The systematic review is aimed at evaluating the value of PRF in different areas of surgery. DATA SOURCES: A systematic review of articles sourced from MEDLINE-pubmed (2008-2017(July)) was done...
August 18, 2017: International Journal of Surgery
https://www.readbyqxmd.com/read/28820322/platelet-rich-fibrin-prf-in-regeneration-of-intrabony-defects-a-randomized-controlled-trial
#20
Gauresh Kumar Patel, Sheela Kumar Gujjari, Veerendra Kumar S C
BACKGROUND AND OBJECTIVE: Platelet-rich fibrin (PRF) is a autologous non transfusional hemo-component with a high concentration of platelets. It incorporates leukocytes, platelets and growth factors within the dense fibrin matrix and can be used as healing biomaterial. This study assessed the adjunctive use of PRF in regenerative management of intrabony defects in comparison with open flap debridement. METHODOLOGY: 26 bilateral defects (13 per group) in 13 patients were randomized as either PRF (Test group) or Open flap debridement alone (control group) sites...
August 18, 2017: Journal of Periodontology
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