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https://www.readbyqxmd.com/read/28439826/surface-acoustic-wave-lysis-and-ion-exchange-membrane-quantification-of-exosomal-microrna
#1
Katherine E Richards, David B Go, Reginald Hill
MicroRNA detection and quantification are commonly explored techniques for diagnostic and prognostic predictions. Typically, microRNAs are extracted and purified from a biological source, converted into complementary DNA (cDNA), and amplified using real time polymerase chain reaction (RT-PCR). The number of RT-PCR cycles required to reach the threshold of detection provides a relative quantification of the target microRNA when this data is normalized to the quantity of a control microRNA. This methodology has several drawbacks, including the need to artificially amplify the target microRNA for detection as well as quantification errors that can occur due to expression level differences of the control microRNAs for normalization in various sample sources...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28438278/complementary-and-alternative-medicine-cam-among-australian-hospital-based-nurses-knowledge-attitude-personal-and-professional-use-reasons-for-use-cam-referrals-and-socio-demographic-predictors-of-cam-users
#2
Seyed Afshin Shorofi, Paul Arbon
OBJECTIVE: This study was intended to examine CAM among Australian hospital-based nurses, identifying their knowledge, attitude, personal and professional use, reasons for use, CAM referrals, and socio-demographic predictors of CAM users. METHODS AND MATERIALS: Nurses holding a qualification in nursing and working in surgical wards were included using a convenience sampling technique. A self-complete questionnaire was developed to achieve the aims of the study. Descriptive and non-parametric statistics were calculated to describe and analyse data...
May 2017: Complementary Therapies in Clinical Practice
https://www.readbyqxmd.com/read/28435032/clinical-efficacy-of-1-year-intensive-systematic-dietary-manipulation-as-complementary-and-alternative-medicine-therapies-on-female-interstitial-cystitis-bladder-pain-syndrome-patients
#3
Hitoshi Oh-Oka
OBJECTIVE: To evaluate the clinical efficacy of intensive systematic dietary manipulation (ISDM) for female patients with interstitial cystitis (IC)/bladder pain syndrome (BPS) in stable condition who were followed in our hospital. MATERIALS AND METHODS: In cooperation with the nutrition control team, we created a basic IC/BPS diet menu for 1 month. Data regarding daily food intake and food-related symptoms were collected by detailed interview of each patient, we set meal menu to control IC/BPS symptoms and advised the patients to reduce the intake of specific food items to the maximum possible extent...
April 20, 2017: Urology
https://www.readbyqxmd.com/read/28434474/chinese-herbal-medicine-adjunct-therapy-in-patients-with-acute-relapse-of-multiple-sclerosis-a-systematic-review-and-meta-analysis
#4
REVIEW
Liang Song, Qi-Hui Zhou, Hui-Lin Wang, Feng-Jiao Liao, Liang Hua, Hong-Feng Zhang, Li-Bo Huang, Yan Lin, Guo-Qing Zheng
BACKGROUND: Many patients with multiple sclerosis (MS) resort to complementary and alternative medicine, which is used in 33%-80% of MS patients in the developed country. The purpose of this study is to assess the efficacy and safety of Chinese herbal medicine (CHM) as an adjunct therapy of patients with acute relapse of MS. METHODS: Six databases were searched for randomized-controlled trial of CHM for acute relapse of MS. The risk of bias was assessed by using the twelve criteria recommended by the Cochrane Back Review Group...
April 2017: Complementary Therapies in Medicine
https://www.readbyqxmd.com/read/28434471/factors-associated-with-complementary-medicine-use-in-pediatric-musculoskeletal-conditions-results-from-a-national-survey
#5
Ezra M Cohen, Michelle L Dossett, Darshan H Mehta, Roger B Davis, Yvonne C Lee
OBJECTIVES: Complementary and alternative medicine (CAM) use is common in children, but its use has only been investigated in children with musculoskeletal conditions (MSK) to a limited extent. We aimed to characterize factors associated with CAM use in children with MSK conditions. METHODS: Within the 2012 National Health Interview Survey dataset (including its child CAM supplement), we examined factors associated with CAM use in children with MSK conditions and performed an analysis examining the perceived usefulness of CAM therapies for MSK conditions...
April 2017: Complementary Therapies in Medicine
https://www.readbyqxmd.com/read/28434470/complementary-and-alternative-medicine-among-hospitalized-pediatric-patients
#6
Adi Oren-Amit, Matitiahu Berkovitch, Hilla Bahat, Michael Goldman, Eran Kozer, Tomer Ziv-Baran, Ibrahim Abu-Kishk
OBJECTIVES: To estimate the prevalence and describe the characteristics of complementary and alternative medicine (CAM) use among hospitalized children, and to discover the awareness of medical staff regarding CAM use. DESIGN/SETTING: Parents of children aged 0-18 years admitted to the Pediatric Division at Assaf Harofeh Medical Center in Israel between January and July of 2015 (n=146) were provided a questionnaire regarding socio-economic status and evaluating the CAM use...
April 2017: Complementary Therapies in Medicine
https://www.readbyqxmd.com/read/28433949/the-dimensional-structure-of-psychopathology-in-22q11-2-deletion-syndrome
#7
Maria Niarchou, Tyler M Moore, Sunny X Tang, Monica E Calkins, Donna M McDonald-McGuinn, Elaine H Zackai, Beverly S Emanuel, Ruben C Gur, Raquel E Gur
BACKGROUND: 22q11.2 Deletion Syndrome (22q11.2DS) is one of the strongest known genetic risk factors for developing schizophrenia. Individuals with 22q11.2DS have high rates of neurodevelopmental disorders in childhood, while in adulthood ∼25% develop schizophrenia. Similar to the general population, high rates of comorbidity are common in 22q11.2DS. Employing a dimensional approach where psychopathology is examined at the symptom-level as complementary to diagnostic categories in a population at such high genetic risk for schizophrenia can help gain a better understanding of how psychopathology is structured as well as its genetic underpinnings...
April 15, 2017: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/28433695/disinfection-of-water-inoculated-with-enterococcus-faecalis-using-solar-fe-iii-edds-h2o2-or-s2o8-2-process
#8
A Bianco, M I Polo López, P Fernández Ibáñez, M Brigante, G Mailhot
In this study, the activation of H2O2 and persulfate ions induced by solar photolysis of Fe(III)EDDS complex were investigated in water disinfection, applying solar AOPs processes. The use of Fe(III)EDDS complex maintains iron in soluble form until slightly basic pH and so the photolysis is efficient in a large range of pH compatible with natural waters. Moreover, for the first time, the impact of photogenerated hydroxyl and sulfate radicals on the inactivation of Enterococcus faecalis in water was studied...
April 18, 2017: Water Research
https://www.readbyqxmd.com/read/28431306/difference-optimization-automatic-correction-of-relative-frequency-and-phase-for-mean-non-edited-and-edited-gaba-1-h-mega-press-spectra
#9
Marianne Cleve, Martin Krämer, Alexander Gussew, Jürgen R Reichenbach
Phase and frequency corrections of magnetic resonance spectroscopic data are of major importance to obtain reliable and unambiguous metabolite estimates as validated in recent research for single-shot scans with the same spectral fingerprint. However, when using the J-difference editing technique (1)H MEGA-PRESS, misalignment between mean edited (ON‾) and non-edited (OFF‾) spectra that may remain even after correction of the corresponding individual single-shot scans results in subtraction artefacts compromising reliable GABA quantitation...
April 7, 2017: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/28426726/vibrotactile-stimulation-a-non-pharmacological-intervention-for-opioid-exposed-newborns
#10
Ian Zuzarte, Premananda Indic, Bruce Barton, David Paydarfar, Francis Bednarek, Elisabeth Bloch-Salisbury
OBJECTIVE: To examine the therapeutic potential of stochastic vibrotactile stimulation (SVS) as a complementary non-pharmacological intervention for withdrawal in opioid-exposed newborns. STUDY DESIGN: A prospective, within-subjects single-center study was conducted in 26 opioid-exposed newborns (>37 weeks; 16 male) hospitalized since birth and treated pharmacologically for Neonatal Abstinence Syndrome. A specially-constructed mattress delivered low-level SVS (30-60Hz, 10-12μm RMS), alternated in 30-min intervals between continuous vibration (ON) and no vibration (OFF) over a 6-8 hr session...
2017: PloS One
https://www.readbyqxmd.com/read/28426356/focus-group-findings-of-smoking-onset-among-male-youth-in-china
#11
Vivian C Sheer, Chang Molly Mao, Yi-Ru Regina Chen
BACKGROUND: Adolescence is associated with smoking initiation among men in China. The lack of qualitative studies using Chinese adolescent samples can pose challenges to enacting effective smoking prevention messages that resonate with male Chinese teenagers' thoughts, needs, and wishes. OBJECTIVE: This focus group study was designed to obtain in-depth contextual information on early smoking among male teenagers in China. METHODS: Twenty focus groups of 7-10 male students from vocational and junior colleges (N = 165) were conducted, approximately half in Shanxi and half in Guangdong...
June 7, 2017: Substance Use & Misuse
https://www.readbyqxmd.com/read/28425415/drug-induced-liver-injury-at-a-tertiary-hospital-in-india-etiology-clinical-features-and-predictors-of-mortality
#12
Chetan Rathi, Nirav Pipaliya, Ruchir Patel, Meghraj Ingle, Aniruddha Phadke, Prabha Sawant
INTRODUCTION AND AIMS: Drug-induced liver injury (DILI) is rare; however, it is one of the important causes of acute liver failure which results in significant morbidity or mortality. MATERIAL AND METHODS: Patients with suspected DILI were enrolled based on predefined criteria and followed up for at least 6 months or until normalization of liver tests. Causality assessment was done by applying the Roussel Uclaf Causality Assessment Method model. RESULTS: We collected data from 82 individuals diagnosed with DILI at our hospital from 2014 through 2015 (41 men; median age, 38 years)...
May 2017: Annals of Hepatology
https://www.readbyqxmd.com/read/28422708/a-brief-essay-on-non-pharmacological-treatment-of-alzheimer-s-disease
#13
Alberto Raggi, Domenica Tasca, Raffaele Ferri
Current pharmacological therapies for Alzheimer's disease (AD) do not modify its course and are not always beneficial. Therefore, the optimization of quality of life represents the best possible outcome achievable in all stages of the disease. Cognitive and behavioural rehabilitation represents the main therapeutic approach for this purpose, also in order to mitigate indirectly the burden of distress of family caregivers. The aim of this mini-review is to go through this theme by discussing cognitive activation, virtual reality and neuromodulation techniques...
April 19, 2017: Reviews in the Neurosciences
https://www.readbyqxmd.com/read/28421118/pattern-of-traditional-medicine-utilization-among-hiv-aids-patients-on-antiretroviral-therapy-at-a-university-hospital-in-northwestern-ethiopia-a-cross-sectional-study
#14
Abyot Endale Gurmu, Fitsum Sebsibe Teni, Wondmagegn Tamiru Tadesse
The objective of this study was to assess traditional, complementary, and alternative medicine (TCAM) utilization pattern among HIV/AIDS patients on antiretroviral therapy at University of Gondar Comprehensive Specialized Hospital. Materials and Methods. Data on sociodemographic profile and clinical and TCAM utilization were collected using a structured, pretested questionnaire from April 01 to May 28, 2014, through interviews with patients. Data on CD4 count, HIV stage, and ART regimen were collected from patient records...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28417094/contemporary-acupressure-therapy-adroit-cure-for-painless-recovery-of-therapeutic-ailments
#15
REVIEW
Piyush Mehta, Vishwas Dhapte, Shivajirao Kadam, Vividha Dhapte
Since time immemorial, complementary and alternative medicines (CAM) have played a significant role in human health care. CAM is known to have a strong reputation and reliability within every culture to provide basic health care treatment for patients. CAM acts as a better therapeutic option in human being for treating various diseases and improving quality of life with apt consideration to the economic aspects. Acupressure, one of the known CAM, originated in ancient China is based on the principal of acupoints activation across the meridians which correct the imbalance between Qi...
April 2017: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/28417090/role-of-traditional-islamic-and-arabic-plants-in-cancer-therapy
#16
REVIEW
Rizwan Ahmad, Niyaz Ahmad, Atta Abbas Naqvi, Adeeb Shehzad, Mastour Safer Al-Ghamdi
ETHNO PHARMACOLOGICAL RELEVANCE: This review article underlines individual Traditional Islamic and Arabic plant (TAI) and their role in treating cancer. The aim of the study is to specifically evaluate the progress of herbs, Arabic and Islamic traditional herbs in particular, applied in cancer treatment, so far. MATERIALS AND METHODS: Islamic and Arabic plants were selected and identified through different literature survey using "Google scholar", "Web of science", "Scopus" and "PubMed"...
April 2017: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/28416612/a-biochemical-and-biophysical-model-of-g-quadruplex-dna-recognition-by-positive-coactivator-of-transcription-4
#17
Wezley C Griffin, Jun Gao, Alicia K Byrd, Shubeena Chib, Kevin D Raney
DNA sequences that are guanine-rich have received considerable attention due to their potential to fold into a secondary, four-stranded DNA structure termed G-quadruplex (G4) which has been implicated in genomic instability and some human diseases. We have previously identified positive coactivator of transcription (PC4), a single-stranded DNA (ssDNA) binding protein, as a novel G4 interactor. Here, to expand on these previous observations, we biochemically and biophysically characterized the interaction between PC4 and G4DNA...
April 17, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28416403/feasibility-of-dsrna-treatment-for-post-clearing-spf-shrimp-stocks-of-newly-discovered-viral-infections-using-laem-singh-virus-lsnv-as-a-model
#18
Vanvimon Saksmerprome, Patai Charoonnart, Timothy W Flegel
Using post-larvae derived from specific pathogen free (SPF) stocks in penaeid shrimp farming has led to a dramatic increase in production. At the same time, new pathogens of farmed shrimp are continually being discovered. Sometimes these pathogens are carried by shrimp and other crustaceans as persistent infections without gross signs of disease. Thus it is that a 5-generation stock of Penaeus monodon SPF for several pathogens was found, post-stock-development, to be persistently-infected with newly-discovered Laem Singh virus (LSNV)...
April 14, 2017: Virus Research
https://www.readbyqxmd.com/read/28413327/effects-of-ginseng-on-two-main-sex-steroid-hormone-receptors-estrogen-and-androgen-receptors
#19
REVIEW
Joonwoo Park, Heewon Song, Si-Kwan Kim, Myeong Soo Lee, Dong-Kwon Rhee, YoungJoo Lee
Ginseng has been used in China for at least two millennia and is now popular in over 35 countries. It is one of the world's popular herbs for complementary and alternative medicine and has been shown to have helpful effects on cognition and blood circulation, as well as anti-aging, anti-cancer, and anti-diabetic effects, among many others. The pharmacological activities of ginseng are dependent mainly on ginsenosides. Ginsenosides have a cholesterol-like four trans-ring steroid skeleton with a variety of sugar moieties...
April 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28413079/mexican-consensus-on-dyspepsia
#20
R Carmona-Sánchez, O Gómez-Escudero, M Zavala-Solares, M V Bielsa-Fernández, E Coss-Adame, A I Hernández-Guerrero, F Huerta-Iga, M E Icaza-Chávez, M A Lira-Pedrín, J A Lizárraga-López, A López-Colombo, A Noble-Lugo, J Pérez-Manauta, R H Raña-Garibay, J M Remes-Troche, J L Tamayo, L F Uscanga, F Zamarripa-Dorsey, M A Valdovinos Díaz, J A Velarde-Ruiz Velasco
Since the publication of the 2007 dyspepsia guidelines of the Asociación Mexicana de Gastroenterología, there have been significant advances in the knowledge of this disease. A systematic search of the literature in PubMed (01/2007 to 06/2016) was carried out to review and update the 2007 guidelines and to provide new evidence-based recommendations. All high-quality articles in Spanish and English were included. Statements were formulated and voted upon using the Delphi method. The level of evidence and strength of recommendation of each statement were established according to the GRADE system...
April 13, 2017: Revista de Gastroenterología de México
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