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Viral trafficking

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https://www.readbyqxmd.com/read/28095450/non-lytic-egression-of-infectious-bursal-disease-virus-ibdv-particles-from-infected-cells
#1
Fernando Méndez, Nicolás Romero, Liliana L Cubas, Laura R Delgui, Dolores Rodríguez, José F Rodríguez
Infectious bursal disease virus (IBDV), a member of the Birnaviridae family, is responsible for a devastating immunosuppressive disease affecting juvenile domestic chickens. IBDV particles are naked icosahedrons enclosing a bipartite double-stranded RNA genome harboring three open reading frames (ORF). One of these ORFs codes for VP5, a non-structural polypeptide dispensable for virus replication in tissue culture but essential for IBDV pathogenesis. Using two previously described recombinant viruses, whose genomes differ in a single nucleotide, expressing or not the VP5 polypeptide, we have analyzed the role of this polypeptide during the IBDV replication process...
2017: PloS One
https://www.readbyqxmd.com/read/28095444/the-role-of-dct-in-hpv16-infection-of-hacats
#2
Pınar Aksoy, Patricio I Meneses
Persistent infection with high-risk human papillomavirus (HPV) genotype is a major factor leading to many human cancers. Mechanisms of HPV entry into host cells and genome trafficking towards the nucleus are incompletely understood. Dopachrome tautomerase (DCT) was identified as a cellular gene required for HPV infection in HeLa cells on a siRNA screen study. Here, we confirm that DCT knockdown significantly decreases HPV infection in the human keratinocyte HaCaT cells as was observed in HeLas. We investigated the effects of DCT knockdown and found that DCT depletion caused increased reactive oxygen species (ROS) levels, DNA damage and altered cell cycle in HaCaT cells...
2017: PloS One
https://www.readbyqxmd.com/read/28077878/pla2g16-represents-a-switch-between-entry-and-clearance-of-picornaviridae
#3
Jacqueline Staring, Eleonore von Castelmur, Vincent A Blomen, Lisa G van den Hengel, Markus Brockmann, Jim Baggen, Hendrik Jan Thibaut, Joppe Nieuwenhuis, Hans Janssen, Frank J M van Kuppeveld, Anastassis Perrakis, Jan E Carette, Thijn R Brummelkamp
Picornaviruses are a leading cause of human and veterinary infections that result in various diseases, including polio and the common cold. As archetypical non-enveloped viruses, their biology has been extensively studied. Although a range of different cell-surface receptors are bound by different picornaviruses, it is unclear whether common host factors are needed for them to reach the cytoplasm. Using genome-wide haploid genetic screens, here we identify the lipid-modifying enzyme PLA2G16 (refs 8, 9, 10, 11) as a picornavirus host factor that is required for a previously unknown event in the viral life cycle...
January 19, 2017: Nature
https://www.readbyqxmd.com/read/28077646/adenovirus-modulates-toll-like-receptor-4-signaling-by-reprogramming-orp1l-vap-protein-contacts-for-cholesterol-transport-from-endosomes-to-the-endoplasmic-reticulum
#4
Nicholas L Cianciola, Stacey Chung, Danny Manor, Cathleen R Carlin
: Human adenoviruses generally cause mild self-limiting infections but can lead to serious disease and even be fatal in high-risk individuals, underscoring the importance of understanding how the virus counteracts host defense mechanisms. This study had two goals. First, determine the molecular basis of cholesterol homeostatic responses induced by the early region 3 membrane protein RIDα via its direct interaction with the sterol-binding protein ORP1L. Second, determine how this interaction regulates innate immunity to adenovirus...
January 11, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28056216/nuclear-localization-and-secretion-competence-is-conserved-amongst-henipavirus-matrix-proteins
#5
Elisabeth C McLinton, Kylie M Wagstaff, Alexander Lee, Gregory W Moseley, Glenn A Marsh, Lin-Fa Wang, David A Jans, Kim G Lieu, Hans Netter
Viruses of the genus Henipavirus of the family Paramyxoviridae are zoonotic pathogens, which have emerged in South East Asia, Australia and Africa. Nipah virus (NiV) and Hendra virus (HeV) are highly virulent pathogens transmitted from bats to animals and humans, whilst the henipavirus Cedar virus (CedV) seems to be non-pathogenic in infection studies. The full replication cycle of the Paramyxoviridae occurs in the host cell's cytoplasm where viral assembly is orchestrated by the matrix (M) protein. Unexpectedly, the NiV-M protein traffics through the nucleus as an essential step to engage the plasma membrane in preparation for viral budding/release...
January 5, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28053097/subcellular-localization-of-hiv-1-gag-pol-mrnas-regulates-sites-of-virion-assembly
#6
Jordan T Becker, Nathan M Sherer
: HIV-1 full-length, unspliced RNAs serve dual roles in the cytoplasm as mRNAs encoding the Gag and Gag-Pol capsid proteins as well as genomic RNAs (gRNAs) packaged by Gag into virions undergoing assembly at the plasma membrane (PM). Because Gag is sufficient to drive assembly of virus-like particles even in the absence of gRNA binding, whether viral RNA trafficking plays an active role in the native assembly pathway is unknown. In this study we tested the effects of modulating the cytoplasmic abundance or distribution of full-length viral RNAs on Gag trafficking and assembly in the context of single cells...
January 4, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28051846/selective-intracellular-delivery-of-ganglioside-gm3-binding-peptide-through-caveolae-raft-mediated-endocytosis
#7
Teruhiko Matsubara, Ryohei Otani, Miki Yamashita, Haruka Maeno, Hanae Nodono, Toshinori Sato
Glycosphingolipids are major components of the membrane raft, and several kinds of viruses and bacterial toxins are known to bind to glycosphingolipids in the membrane raft. Since the viral genes and pathogenic proteins that are taken into cells are directly delivered to their target organelles, caveolae/raft-mediated endocytosis represents a promising pathway for specific delivery. In the present study, we demonstrated the ability of an artificial pentadecapeptide, which binds to ganglioside GM3, to deliver protein into cells by caveolae/raft-mediated endocytosis...
January 4, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28031358/association-of-human-papillomavirus-type-16-e2-with-rad50-interacting-protein-1-enhances-viral-dna-replication
#8
Karen Campos León, Kalpanee Wijendra, Abida Siddiqa, Ieisha Pentland, Katherine M Feeney, Alison Knapman, Rachel Davies, Elliot J Androphy, Joanna L Parish
: Rad50-interacting protein 1 (Rint1) associates with the DNA damage response protein Rad50 during the S phase to G2/M transition and functions in radiation-induced G2 checkpoint control. It has also been demonstrated that Rint1 is essential in vesicle trafficking from the Golgi to the endoplasmic reticulum (ER) through interaction with Zeste-White 10 (ZW10). We have isolated a novel interaction between Rint1 and the human papillomavirus type 16 (HPV16) transcription and replication factor E2...
December 28, 2016: Journal of Virology
https://www.readbyqxmd.com/read/28004015/influenza-infection-modulates-vesicular-trafficking-and-induces-golgi-complex-disruption
#9
Vibha Yadav, Antonito T Panganiban, Kerstin Honer Zu Bentrup, Thomas G Voss
Influenza A virus (IFV) replicates its genome in the nucleus of infected cells and uses the cellular protein transport system for genome trafficking from the nucleus to the plasma membrane. However, many details of the mechanism of this process, and its relationship to subsequent cytoplasmic virus trafficking, have not been elucidated. We examined the effect of nuclear transport inhibitors Leptomycin B (LB), 5,6 dichloro-1-β-d-ribofuranosyl-benzimidazole (DRB), the vesicular transport inhibitor Brefeldin A (BFA), the caspase inhibitor ZWEHD, and microtubule inhibitor Nocodazole (NOC) on virus replication and intracellular trafficking of viral nucleoprotein (NP) from the nucleus to the ER and Golgi...
December 2016: Virusdisease
https://www.readbyqxmd.com/read/28003809/cytotoxic-cd4-t-cells-differentiation-function-and-application-to-dengue-virus-infection
#10
REVIEW
Yuan Tian, Alessandro Sette, Daniela Weiskopf
Dengue virus (DENV) has spread through most tropical and subtropical areas of the world and represents a serious public health problem. The control of DENV infection has not yet been fully successful due to lack of effective therapeutics or vaccines. Nevertheless, a better understanding of the immune responses against DENV infection may reveal new strategies for eliciting and improving antiviral immunity. T cells provide protective immunity against various viral infections by generating effector cells that cooperate to eliminate antigens and memory cells that can survive for long periods with enhanced abilities to control recurring pathogens...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27987184/look-who-s-talking-the-crosstalk-between-oxidative-stress-and-autophagy-supports-exosomal-dependent-release-of-hcv-particles
#11
REVIEW
Regina Medvedev, Eberhard Hildt, Daniela Ploen
Autophagy is a highly conserved and regulated intracellular lysosomal degradation pathway that is essential for cell survival. Dysregulation has been linked to the development of various human diseases, including neurodegeneration and tumorigenesis, infection, and aging. Besides, many viruses hijack the autophagosomal pathway to support their life cycle. The hepatitis C virus (HCV), a major cause of chronic liver diseases worldwide, has been described to induce autophagy. The autophagosomal pathway can be further activated in response to elevated levels of reactive oxygen species (ROS)...
December 16, 2016: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/27984059/cruising-the-cellular-highways-how-human-papillomavirus-travels-from-the-surface-to-the-nucleus
#12
REVIEW
Stephen DiGiuseppe, Malgorzata Bienkowska-Haba, Lucile G Guion, Martin Sapp
The non-enveloped human papillomaviruses (HPVs) specifically target epithelial cells of the skin and mucosa. Successful infection requires a lesion in the stratified tissue for access to the basal cells. Herein, we discuss our recent progress in understanding binding, internalization, uncoating, and intracellular trafficking of HPV particles. Our focus will be on HPV type 16, which is the most common HPV type associated with various anogenital and oropharyngeal carcinomas. The study of HPV entry has revealed a number of novel cellular pathways utilized during infection...
October 28, 2016: Virus Research
https://www.readbyqxmd.com/read/27975305/live-cell-imaging-confocal-microscopy-analysis-of-hbv-myr-pres1-peptide-binding-and-uptake-in-ntcp-gfp-expressing-hepg2-cells
#13
Alexander König, Dieter Glebe
To obtain basic knowledge about specific molecular mechanisms involved in the entry of pathogens into cells is the basis for establishing pharmacologic substances blocking initial viral binding, infection, and subsequent viral spread. Lack of information about key cellular factors involved in the initial steps of HBV infection has hampered the characterization of HBV binding and entry for decades. However, recently, the liver-specific sodium-dependent taurocholate cotransporting polypeptide (NTCP) has been discovered as a functional receptor for HBV and HDV, thus opening the field for new concepts of basic binding and entry of HBV and HDV...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27974567/hcmv-requires-egfr-signaling-to-enter-and-initiate-the-early-steps-in-the-establishment-of-latency-in-cd34-human-progenitor-cells
#14
Jung Heon Kim, Donna Collins-McMillen, Jason C Buehler, Felicia D Goodrum, Andrew D Yurochko
: The establishment of human cytomegalovirus (HCMV) latency and persistence relies on the successful infection of hematopoietic cells, which serve as sites of viral persistence and contribute to viral spread. Here, using blocking antibodies and pharmacological inhibitors, we document that HCMV activation of the epidermal growth factor receptor (EGFR) and downstream phosphatidylinositol-3-kinase (PI (3)K) mediates viral entry into CD34+ human progenitor cells (HPCs), resulting in distinct cellular trafficking and nuclear translocation of the virus compared to other immune cells, such as we have documented in monocytes...
December 14, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27974566/cd63-regulates-epstein-barr-virus-lmp1-exosomal-packaging-enhancement-of-vesicle-production-and-non-canonical-nf-%C3%AE%C2%BAb-signaling
#15
Stephanie N Hurwitz, Dingani Nkosi, Meghan M Conlon, Sara B York, Xia Liu, Deanna C Tremblay, David G Meckes
: Latent membrane protein 1 (LMP1) is an Epstein-Barr virus (EBV) encoded oncoprotein that is packaged into small extracellular vesicles (EVs) called exosomes. Trafficking of LMP1 into multivesicular bodies (MVBs) alters the content and function of exosomes. LMP1-modified exosomes enhance the growth, migration, and invasion of malignant cells, demonstrating the capacity to manipulate the tumor microenvironment and enhance the progression of EBV-associated cancers. Despite the growing evidence surrounding the significance of LMP1-modified exosomes in cancer, very little is understood about the mechanisms that orchestrate LMP1 incorporation into these vesicles...
December 14, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27974560/zika-virus-infects-early-and-mid-gestation-human-maternal-decidual-tissues-inducing-distinct-innate-tissue-responses-in-the-maternal-fetal-interface
#16
Yiska Weisblum, Esther Oiknine-Djian, Olesya M Vorontsov, Ronit Haimov-Kochman, Zichria Zakay-Rones, Karen Meir, David Shveiky, Sharona Elgavish, Yuval Nevo, Moshe Roseman, Michal Bronstein, David Stockheim, Ido From, Iris Eisenberg, Aya A Lewkowicz, Simcha Yagel, Amos Panet, Dana G Wolf
: Zika virus (ZIKV) has emerged as a cause of congenital brain anomalies and a range of placental-related abnormalities, highlighting the need to unveil the modes of maternal-to-fetal transmission. The most likely route of vertical ZIKV transmission is via the placenta. The earliest events of ZIKV transmission in the maternal decidua, representing the maternal-uterine aspect of the chimeric placenta, have remained unexplored. Here we show that ZIKV replicates in first-trimester human maternal-decidual tissues grown ex-vivo as 3D organ cultures...
December 14, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27973418/varicella-zoster-virus-infectious-cycle-er-stress-autophagic-flux-and-amphisome-mediated-trafficking
#17
REVIEW
Charles Grose, Erin M Buckingham, John E Carpenter, Jeremy P Kunkel
Varicella-zoster virus (VZV) induces abundant autophagy. Of the nine human herpesviruses, the VZV genome is the smallest (~124 kbp), lacking any known inhibitors of autophagy, such as the herpes simplex virus ICP34.5 neurovirulence gene. Therefore, this review assesses the evidence for VZV-induced cellular stress, endoplasmic-reticulum-associated degradation (ERAD), and autophagic flux during the VZV infectious cycle. Even though VZV is difficult to propagate in cell culture, the biosynthesis of the both N- and O-linked viral glycoproteins was found to be abundant...
December 10, 2016: Pathogens
https://www.readbyqxmd.com/read/27930721/both-cerebral-and-hematopoietic-deficiencies-in-ccr2-result-in-uncontrolled-herpes-simplex-virus-infection-of-the-central-nervous-system-in-mice
#18
Rafik Menasria, Coraline Canivet, Jocelyne Piret, Jean Gosselin, Guy Boivin
CCR2 is a chemokine receptor expressed on the surface of blood leukocytes, particularly «Ly6Chi» inflammatory monocytes and microglia. Signaling through this receptor is thought to influence the immune activity of microglia as well as monocytes egress from the bone marrow (BM) and their trafficking into the central nervous system (CNS) in several neurological diseases. During experimental herpes simplex virus 1 (HSV-1) encephalitis (HSE), CCR2 deficiency has been reported to exacerbate the outcome of the disease...
2016: PloS One
https://www.readbyqxmd.com/read/27928009/influence-of-plasma-cell-niche-factors-on-the-recruitment-and-maintenance-of-irf4hi-plasma-cells-and-plasmablasts-in-vaccinated-siv-infected-rhesus-macaques-with-low-and-high-viremia
#19
Julia M Shaw, Leia K Miller-Novak, Venkatramanan Mohanram, Katherine McKinnon, Thorsten Demberg, Diego A Vargas-Inchaustegui, David Venzon, Marjorie Robert-Guroff
: In a recent study, we found protection following SIV exposure correlated with rectal plasma cell frequency in vaccinated female rhesus macaques. We sought to determine if these same macaques maintained high mucosal plasma cell frequencies post-infection and if this translated to reduced viremia. Although delayed SIV acquisition did not predict subsequent viral control, alterations existed in the distribution of plasma cells and plasmablasts between macaques that exhibited high or low viremia...
December 7, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27911820/map4-regulated-dynein-dependent-trafficking-of-btn3a1-controls-the-tbk1-irf3-signaling-axis
#20
Minji Seo, Seong-Ok Lee, Ji-Hoon Kim, Yujin Hong, Seongchan Kim, Yeumin Kim, Dal-Hee Min, Young-Yun Kong, Jinwook Shin, Kwangseog Ahn
The innate immune system detects viral nucleic acids and induces type I interferon (IFN) responses. The RNA- and DNA-sensing pathways converge on the protein kinase TANK-binding kinase 1 (TBK1) and the transcription factor IFN-regulatory factor 3 (IRF3). Activation of the IFN signaling pathway is known to trigger the redistribution of key signaling molecules to punctate perinuclear structures, but the mediators of this spatiotemporal regulation have yet to be defined. Here we identify butyrophilin 3A1 (BTN3A1) as a positive regulator of nucleic acid-mediated type I IFN signaling...
December 13, 2016: Proceedings of the National Academy of Sciences of the United States of America
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