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https://www.readbyqxmd.com/read/28726547/three-dimensional-bio-printing-of-hepatic-structures-with-direct-converted-hepatocyte-like-cells
#1
Kyojin Kang, Yohan Kim, Seung Bum Lee, Ji Sook Kim, Sua Park, Wan-Doo Kim, Heung-Mo Yang, Sung-Joo Kim, Jaemin Jeong, Dongho Choi
Three-dimensional (3D) bio-printing technology is a promising new technology in the field of bio-artificial organ generation with regard to overcoming the limitations of organ supply. The cell source for bio-printing is very important. Here, we generated 3D hepatic scaffold with mouse induced hepatocyte-like cells (miHeps), and investigated whether their function was improved after transplantation in vivo. To generate miHeps, mouse embryonic fibroblasts were transformed with pMX retroviruses individually expressing hepatic transcription factors Hnf4a and Foxa3...
July 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28724609/thy-1-fibroblast-subsets-in-the-human-peritoneum
#2
Edyta Kawka, Janusz Witowski, Maria Bartosova, Rusan Catar, Andras Rudolf, Aurelie Philippe, Rafal Rutkowski, Betti Schaefer, Claus Peter Schmitt, Duska Dragun, Achim Joerres
Fibrotic thickening of the peritoneum develops in patients receiving peritoneal dialysis (PD) for renal failure. For unknown reasons, however, in some patients it progresses to extensive fibrosis that compromises dialysis capacity of the peritoneum. It is increasingly clear that fibroblasts display large heterogeneity not only between but also within tissues. Differential surface expression of glycoprotein Thy-1 (CD90) has been shown to identify functionally distinct fibroblast subsets in several organs. Here, we isolated Thy-1+/- subsets of human peritoneal fibroblasts (HPFB) and analyzed them in terms of pro-fibrotic myofibroblast features...
July 19, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28723751/early-laparotomy-and-timely-reconstruction-for-patients-with-abdominal-electrical-injury-five-case-reports-and-literature-review
#3
Pi-Hong Zhang, Zan Liu, Li-Cheng Ren, Ji-Zhang Zeng, Geng-Wen Huang, Mu-Zhang Xiao, Jie Zhou, Peng-Fei Liang, Ming-Hua Zhang, Xiao-Yuan Huang
INTRODUCTION: High-tension electricity can cause devastating injuries that may result in abdominal wall loss, visceral damage, and sometimes major threat to life. The visceral organ may be exposed after debridement and require flap cover, but the tensile strength of abdominal wall may be lack even if flap transplanted. METHODS: From April 2007 through May 2015, 5 patients with severe abdominal electrical injury were treated at our hospital. Exploratory laparotomy was performed based on their clinical manifestations and debridement findings of abdominal wall at early stage, and decision regarding technique for reconstruction of abdominal wall was based on an assessment of the location and extent of the defect...
July 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28721189/strangulated-gallbladder-caused-by-a-tip-of-the-omentum-attached-to-the-abdominal-wall-a-case-report
#4
Atsushi Kohga, Takuya Okumura, Kenji Suzuki, Kiyoshige Yajima, Kimihiro Yamashita, Jun Isogaki, Akihiro Kawabe, Taizo Kimura
Strangulation of the gallbladder associated with the omentum is extremely rare and, to our knowledge, only two cases have been previously reported. The patient was a 91-year-old female who presented to our emergency room with a chief complaint of upper abdominal pain. Computed tomography imaging revealed a gourd-shaped gallbladder with biphasic appearance divided clearly at the body. In the fundus side, the wall of the gallbladder was oedematous and thickened, while the neck side was intact. On the second day after onset, a laparoscopic cholecystectomy was performed...
July 2017: Journal of Surgical Case Reports
https://www.readbyqxmd.com/read/28708772/successful-laparoscopic-harvesting-of-omental-pedicle-flap-for-salvage-cerebral-revascularization-in-2-adults-with-moyamoya-disease-technique-and-lessons-learned
#5
Max Schumm, Kathleen Simon, Rapheal Sacho, Jon C Gould
Moyamoya is an idiopathic and progressive disease that leads to occlusion of intracranial arteries. When other methods of revascularization fail, omentum can be used as an indirect bypass to improve cerebral perfusion. Laparoscopic mobilization of a pedicled omental flap with subcutaneous tunneling to the brain has been described in small case series in children. We report our techniques and outcomes in 2 adults with Moyamoya to undergo such a procedure. An omental flap based on the right gastroepiploic artery was created and intraoperative fluorescence imaging using indocyanine green was used to assess the viability of the flap and to guide lengthening of the pedicle...
July 13, 2017: Surgical Laparoscopy, Endoscopy & Percutaneous Techniques
https://www.readbyqxmd.com/read/28700426/coexisting-primary-ovarian-and-omental-hydatid-disease-mimicking-an-ovarian-neoplasm-a-case-report
#6
Emre E Tas, Gulin F Yegin Akcay, Fatma Yildirim, Filiz Yavuz
Hydatid disease is a parasitic infection that most commonly affects the liver and lungs, although the disease can arise in any part of the body. Cysts may mimic many benign and malignant conditions. The diagnosis cannot be confirmed preoperatively in all cases. A 44-yr-old menopausal woman was admitted to the department of gynecology with complaints of abdominal distention. A fixed abdominopelvic mass was identified. Radiology revealed a 20-cm mass with branched septations and solid components. CA-125 level was 55 kU/L, and Risk of Malignancy Index-2 score was 880...
July 11, 2017: International Journal of Gynecological Pathology
https://www.readbyqxmd.com/read/28698469/potential-of-integrin-inhibitors-for-treating-ovarian-cancer-a-literature-review
#7
REVIEW
Masaki Kobayashi, Kenjiro Sawada, Tadashi Kimura
Epithelial ovarian cancer is a fatal disease, with a cure rate of only 30%. Several recent studies have targeted integrins for cancer treatment. Preclinical studies have shown the effectiveness of several integrin inhibitors for blocking cancer progression, especially by blocking angiogenesis. Because the initial critical step in ovarian cancer metastasis is the attachment of cancer cells to the peritoneum or omentum and because clinical trials have provided positive results for anti-angiogenic therapy, therapies targeting integrins may be the most feasible approach for treating cancer...
July 8, 2017: Cancers
https://www.readbyqxmd.com/read/28690123/pedicled-omental-flaps-in-the-treatment-of-complex-spinal-wounds-after-en-bloc-resection-of-spine-tumors
#8
Andrea Sambri, Alessandro Gasbarrini, Sergio Cialdella, Pierandrea De Iaco, Stefano Boriani
STUDY DESIGN: The present paper presents a retrospective study of 5 patients who underwent pedicled omental flap procedure following spine tumor removal. SUMMARY OF BACKGROUND DATA: Postoperative wound dehiscence represents a major complication in spinal surgery, particularly after en bloc tumor resection, because of the extended sacrifice of soft tissues and adjuvant radiation therapy and chemotherapy. METHODS: Five patients, with a mean age of 52 years (range, 24-71 years), who underwent omental flaps for the treatment of postoperative complication in spine tumor resections were retrospectively evaluated...
June 16, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/28687504/infected-abdominal-aortic-aneurysms-treated-with-extra-anatomic-prosthesis-bypass-in-the-retroperitoneum
#9
You-Fei Qi, Zhan-Xiang Xiao, Chang Shu, Jie Yue, Sa-Hua Liu, Hao Chen, Zhao-Fan Zeng, Wen-Bo Zhang
BACKGROUND: Infected abdominal aortic aneurysms (iAAAs) are rare but life-threatening diseases. The purpose of the present study was to report our experience of extra-anatomic prosthesis bypass in the retroperitoneum as a treatment for iAAAs. METHODS: Data of 8 consecutive patients diagnosed with iAAAs and treated by an extra-anatomic prosthesis bypass in the retroperitoneum were retrospectively collected. Operative details were as follows: one side of the retroperitoneal space was selected to build a track and a bifurcated ePTFE prosthesis was placed through the track...
July 4, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28680782/internal-hernia-of-the-greater-omentum-cadaveric-findings-of-a-previously-unreported-variant
#10
Andrea Andrea, Vy Tran, Cameron K Schmidt, Christian Fisahn, Joe Iwanaga, Rod J Oskouian, R Shane Tubbs
Transomental hernias (TOHs) are a rare finding, constituting a fraction of all intestinal hernias. Here, we report the cadaveric discovery of a spontaneous TOH involving the sigmoid colon in an 82-year-old female and discuss the relevant literature. To our knowledge, a TOH involving the sigmoid colon has not been previously reported.
May 30, 2017: Curēus
https://www.readbyqxmd.com/read/28675934/characterization-of-the-extracellular-matrix-of-normal-and-diseased-tissues-using-proteomics
#11
Alexandra Naba, Oliver Mt Pearce, Amanda Del Rosario, Duanduan Ma, Huiming Ding, Vinothini Rajeeve, Pedro R Cutillas, Frances R Balkwill, Richard O Hynes
The extracellular matrix (ECM) is a complex meshwork of insoluble fibrillar proteins and signaling factors interacting together to provide architectural and instructional cues to the surrounding cells. Alterations in ECM organization or composition and excessive ECM deposition have been observed in diseases such as fibrosis, cardio-vascular diseases, and cancer. We provide here optimized protocols to solubilize ECM proteins from normal or tumor tissues, digest the proteins into peptides, analyze ECM peptides by mass spectrometry, and interpret the mass spectrometric data...
July 4, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28662988/the-rare-extragonadal-omental-teratoma-a-case-report
#12
Fabio Rampinelli, Paolo Donarini, Chiara Visenzi, Silvia Ficarelli, Angela Gambino, Giuseppe Ciravolo
Teratomas of extragonadal origin are extremely rare, and the most common extragonadal site to find teratomas is the omentum. Teratomas are typically found in women of reproductive age but they are also seen in young girls and postmenopausal women. Generally, teratomas arise from germ cells that may induce different cells to originate from the three primitive embryonic layers. Three main theories have been proposed to explain their location. The present report summarizes these theories as well as describes a case of mature cystic teratoma of the omentum that was managed by laparoscopic resection...
June 26, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28662703/the-diagnosis-of-a-metastatic-breast-tumor-from-ovarian-cancer-by-the-succession-of-a-p53-mutation-a-case-report
#13
Ryutaro Mori, Manabu Futamura, Kasumi Morimitsu, Chiemi Saigo, Tatsuhiko Miyazaki, Kazuhiro Yoshida
BACKGROUND: Metastatic breast tumors from other organs are very rare. We herein describe the case of a patient with a metastatic breast tumor due to ovarian cancer who was diagnosed by the succession of a p53 mutation. CASE PRESENTATION: The patient was a 59-year-old woman with sigmoid colon stenosis. Diagnostic imaging revealed a pelvic mass, multiple liver tumors, ascites, and multiple swollen para-aortic lymph nodes, suggesting an advanced ovarian tumor. Transverse loop colostomy and partial resection of the greater omentum was performed followed by six cycles of paclitaxel with carboplatin chemotherapy (TC therapy)...
June 29, 2017: World Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28660813/monophasic-synovial-sarcoma-of-the-greater-omentum-case-report-and-review-of-literature
#14
F J Alcalá Serrano, J R Hernández Hernández, T Montenegro Dámaso, E López-Tomassetti Fernández
Synovial sarcoma is a malignant spindle cell neoplasm normally arising from tissues around joints, bursa and tendon sheaths. Several reports involving the gastrointestinal tract, mainly the oesophagus and stomach, have been documented; however, the omentum remains an extremely unusual location. Monophasic type is composed exclusively of spindle cells arranged in fascicles. Establishing the correct diagnosis of these tumours could be challenging because of the similarities with gastrointestinal stromal tumours and other mesenchymal tumours with similar histology...
July 2017: Annals of the Royal College of Surgeons of England
https://www.readbyqxmd.com/read/28656126/hernia-of-morgagni-presenting-as-constipation-in-a-65-year-old-male
#15
Hamza A Khan, Adeel Samad, Omar A Khan, Farida K Chagan, Jamal K Khan, Saulat H Fatimi
Hernia of Morgagni is congenital defects in the diaphragm. They are mostly asymptomatic and present with vague symptoms when they do so. A high index of suspicion is required for timely diagnose of this condition. Here we present the case of a 65-year-old male patient presented to our institute with constipation for the past six months. Chest radiology raised the suspicion of a hernia which was further confirmed by contrast studies. Laparotomy was done and the hernia sac identified, colon and greater omentum reduced and defect repaired...
May 26, 2017: Curēus
https://www.readbyqxmd.com/read/28653858/transplantation-of-a-human-tissue-engineered-bowel-in-an-athymic-rat-model
#16
Elie Zakhem, Riccardo Tamburrini, Giuseppe Orlando, Kenneth L Koch, Khalil N Bitar
Intestinal failure is a serious clinical condition characterized by loss of motility, absorptive function, and malnutrition. Current treatments do not provide the optimal solution for patients due to the numerous resulting complications. A bioengineered bowel that contains the necessary cellular components provides a viable option for patients. In this study, human tissue-engineered bowel (hTEB) was developed using a technique, whereby human-sourced smooth muscle cells were aligned and neoinnervated using human-sourced neural progenitor cells, resulting in the formation of intrinsically innervated smooth muscle sheets...
June 27, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28651922/peritoneal-carcinomatosis-in-primary-ovarian-cancer-ultrasound-detection-and-comparison-with-computed-tomography
#17
Zhenhong Qi, Yixiu Zhang, Qing Dai, Yu Xia, Yuxin Jiang
We retrospectively compared detection rates and consistency for diagnosis of peritoneal carcinomatosis (PC) of primary ovarian cancer (OC) between ultrasound (US) and computed tomography (CT) scans in 41 patients whose PC of OC (stages IIC-IV) had been diagnosed by histopathology findings. Compared with CT detection rates, those for US were significantly higher for metastases to the pelvic area (92.3% vs. 43.6%, p < 0.001) and bowel surface (64.0% vs. 16.0%, p = 0.002); however, they did not significantly differ for other sites: omentum, diaphragm, lateral peritoneum, mesenteric, hepatic and splenic surfaces...
June 23, 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28649123/extraovarian-primary-peritoneal-carcinomatosis-a-case-report
#18
Gregory Nicolas, Tony Kfoury, Hassan Fawaz, Mahdi Issa
BACKGROUND Extraovarian primary peritoneal carcinoma (EOPPC) is a rare malignant epithelial tumor with an age-adjusted incidence rate of 6.78 per million, which arises from the peritoneal lining with minimal or no ovarian involvement. EOPPC is a diagnosis of exclusion with the absence of other identifiable primary sites and after surgical assessment and consideration of the histological report to evaluate the extent of ovarian invasion. CASE REPORT A 66-year-old woman complained of mild postprandial epigastric pain...
June 26, 2017: American Journal of Case Reports
https://www.readbyqxmd.com/read/28646385/imaging-of-the-pediatric-peritoneum-mesentery-and-omentum
#19
Jonathan R Dillman, Ethan A Smith, Ajaykumar C Morani, Andrew T Trout
The normal peritoneal structures, including the mesenteries and the omenta, are only a few cell layers thick and are visible on imaging based upon the tissues (e.g., fat) and structures (e.g., blood vessels and lymph nodes) contained within them. These structures become more visible and change in appearance when involved by pathological processes. In this pictorial essay, we discuss the normal anatomy of the various abdominopelvic peritoneal structures and illustrate numerous developmental and acquired diagnoses that involve these structures in the pediatric and young adult population...
July 2017: Pediatric Radiology
https://www.readbyqxmd.com/read/28632775/unique-cellular-interactions-between-pancreatic-cancer-cells-and-the-omentum
#20
Valerya Feygenzon, Shelly Loewenstein, Nir Lubezky, Metsada Pasmanic-Chor, Osnat Sher, Joseph M Klausner, Guy Lahat
Pancreatic cancer is a common cause of cancer-related mortality. Omental spread is frequent and usually represents an ominous event, leading to patient death. Omental metastasis has been studied in ovarian cancer, but data on its role in pancreatic cancer are relatively scarce and the molecular biology of this process has yet to be explored. We prepared tissue explants from human omental fat, and used conditioned medium from the explants for various in vitro and in vivo experiments designed to evaluate pancreatic cancer development, growth, and survival...
2017: PloS One
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