keyword
MENU ▼
Read by QxMD icon Read
search

spheroid

keyword
https://www.readbyqxmd.com/read/28925549/biomaterial-substrate-mediated-multicellular-spheroid-formation-and-their-applications-in-tissue-engineering
#1
REVIEW
Ting-Chen Tseng, Chui-Wei Wong, Fu-Yu Hsieh, Shan-Hui Hsu
Three-dimentional (3D) multicellular aggregates (spheroids), compared to the traditional 2D monolayer cultured cells, are physiologically more similar to the cells in vivo. So far there are various techniques to generate 3D spheroids. Spheroids obtained from different methods have already been applied to regenerative medicine or cancer research. Among the cell spheroids created by different methods, the substrate-derived spheroids and their forming mechanism are unique. This review focuses on the formation of biomaterial substrate-mediated multicellular spheroids and their applications in tissue engineering and tumor models...
September 19, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28923372/in-vitro-tubulogenesis-of-madin-darby-canine-kidney-mdck-spheroids-occurs-depending-on-constituent-cell-number-and-scaffold-gel-concentration
#2
Tsuyoshi Hirashima, Miho Hoshuyama, Taiji Adachi
In vitro tubulogenesis has been employed as an experimental model system to study tissue morphogenesis of internal organs. It has been previously shown that Madin-Darby canine kidney (MDCK) cells form tubes in the presence of hepatocyte growth factor in 3D cultures. Although these cells are expected to form tube structures in some microenvironments independent of chemical stimulation, little is known about the cellular mechanisms in organizing such an anisotropic multicellular structure. Here, we report 3D culture conditions that induce MDCK tubulogenesis without growth factor stimulation...
September 15, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28922975/bioengineered-submucosal-organoids-for-in-vitro-modeling-of-colorectal-cancer
#3
Mahesh Devarasetty, Aleksander Skardal, Kyle Cowdrick, Frank Marini, Shay Soker
The physical nature of the tumor microenvironment significantly impacts tumor growth, invasion and response to drugs. Most in vitro tumor models are designed to study the effects of extracellular matrix (ECM) stiffness on tumor cells, while not addressing the effects of ECM's specific topography. In this study we bioengineered submucosal organoids, using primary smooth muscle cells (SMCs) embedded in collagen I (Col I) hydrogel, which produce aligned and parallel fiber topography similar to those found in vivo...
September 19, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28922772/plasmolysis-deplasmolysis-causes-changes-in-endoplasmic-reticulum-form-movement-flow-and-cytoskeletal-association
#4
Xiaohang Cheng, Ingeborg Lang, Opeyemi Samson Adeniji, Lawrence Griffing
Plasmolysis of hypocotyl cells of transgenic Arabidopsis thaliana and Nicotiana benthamiana diminishes the dynamics of the remodeling of the endoplasmic reticulum (ER) in the central protoplast, namely that withdrawn from the cell wall, and more persistent cisternae are formed, yet little change in the actin network in the protoplast occurs. Also, protein flow within the ER network in the protoplast, as detected with fluorescence recovery after photobleaching (FRAP), is not affected by plasmolysis. After plasmolysis, another network of strictly tubular ER remains attached to the plasma membrane-wall interface and is contained within the Hechtian strands and reticulum...
July 10, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28922351/estimation-of-the-mechanical-properties-of-the-eye-through-the-study-of-its-vibrational-modes
#5
M Á Aloy, J E Adsuara, P Cerdá-Durán, M Obergaulinger, J J Esteve-Taboada, T Ferrer-Blasco, R Montés-Micó
Measuring the eye's mechanical properties in vivo and with minimally invasive techniques can be the key for individualized solutions to a number of eye pathologies. The development of such techniques largely relies on a computational modelling of the eyeball and, it optimally requires the synergic interplay between experimentation and numerical simulation. In Astrophysics and Geophysics the remote measurement of structural properties of the systems of their realm is performed on the basis of (helio-)seismic techniques...
2017: PloS One
https://www.readbyqxmd.com/read/28921817/diagnostic-criteria-for-adult-onset-leukoencephalopathy-with-axonal-spheroids-and-pigmented-glia-due-to-csf1r-mutation
#6
Takuya Konno, Kunihiro Yoshida, Ikuko Mizuta, Toshiki Mizuno, Toshitaka Kawarai, Masayoshi Tada, Hiroaki Nozaki, Shu-Ichi Ikeda, Osamu Onodera, Zbigniew K Wszolek, Takeshi Ikeuchi
BACKGROUND: To establish and validate diagnostic criteria for adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP) due to colony stimulating factor 1 receptor (CSF1R) mutation. METHODS: We developed diagnostic criteria for ALSP based on a recent analysis of the clinical characteristics of ALSP. These criteria provide "probable" and "possible" designations for patients who do not have a genetic diagnosis. To verify its sensitivity and specificity, we retrospectively applied our criteria to 83 ALSP cases who had CSF1R mutations (24 of these were analyzed at our institutions, and the others were identified from the literature), 53 cases who had CSF1R mutation-negative leukoencephalopathies, and 32 cases who had cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) with NOTCH3 mutations...
September 18, 2017: European Journal of Neurology: the Official Journal of the European Federation of Neurological Societies
https://www.readbyqxmd.com/read/28921421/tissue-engineering-of-3d-organotypic-microtissues-by-acoustic-assembly
#7
Yuqing Zhu, Vahid Serpooshan, Sean Wu, Utkan Demirci, Pu Chen, Sinan Güven
There is a rapidly growing interest in generation of 3D organotypic microtissues with human physiologically relevant structure, function, and cell population in a wide range of applications including drug screening, in vitro physiological/pathological models, and regenerative medicine. Here, we provide a detailed procedure to generate structurally defined 3D organotypic microtissues from cells or cell spheroids using acoustic waves as a biocompatible and scaffold-free tissue engineering tool.
September 19, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28919991/umbilical-cord-blood-cd34-progenitor-derived-nk-cells-efficiently-kill-ovarian-cancer-spheroids-and-intraperitoneal-tumors-in-nod-scid-il2rg-null-mice
#8
Janneke S Hoogstad-van Evert, Jeannette Cany, Dirk van den Brand, Manon Oudenampsen, Roland Brock, Ruurd Torensma, Ruud L Bekkers, Joop H Jansen, Leon F Massuger, Harry Dolstra
Adoptive transfer of allogeneic natural killer (NK) cells is an attractive therapy approach against ovarian carcinoma. Here, we evaluated the potency of highly active NK cells derived from human CD34+ haematopoietic stem and progenitor cells (HSPC) to infiltrate and mediate killing of human ovarian cancer spheroids using an in vivo-like model system and mouse xenograft model. These CD56+Perforin+ HSPC-NK cells were generated under stroma-free conditions in the presence of StemRegenin-1, IL-15, and IL-12, and exerted efficient cytolytic activity and IFNγ production toward ovarian cancer monolayer cultures...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28919558/cellular-uptake-of-extracellular-vesicles-is-mediated-by-clathrin-independent-endocytosis-and-macropinocytosis
#9
Helena Costa Verdera, Jerney J Gitz-Francois, Raymond M Schiffelers, Pieter Vader
Recent evidence has established that extracellular vesicles (EVs), including exosomes and microvesicles, form an endogenous transport system through which biomolecules, including proteins and RNA, are exchanged between cells. This endows EVs with immense potential for drug delivery and regenerative medicine applications. Understanding the biology underlying EV-based intercellular transfer of cargo is of great importance for the development of EV-based therapeutics. Here, we sought to characterize the cellular mechanisms involved in EV uptake...
September 14, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28917911/injectable-glycosaminoglycan-protein-nano-complex-in-semi-interpenetrating-networks-a-biphasic-hydrogel-for-hyaline-cartilage-regeneration
#10
Janani Radhakrishnan, Anuradha Subramanian, Swaminathan Sethuraman
Articular hyaline cartilage regeneration remains challenging due to its less intrinsic reparability. The study develops injectable biphasic semi-interpenetrating polymer networks (SIPN) hydrogel impregnated with chondroitin sulfate (ChS) nanoparticles for functional cartilage restoration. ChS loaded zein nanoparticles (∼150nm) prepared by polyelectrolyte-protein complexation were interspersed into injectable SIPNs developed by blending alginate with poly(vinyl alcohol) and calcium crosslinking. The hydrogel exhibited interconnected porous microstructure (39...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28916296/folate-targeted-amphiphilic-cyclodextrin-nanoparticles-incorporating-a-fusogenic-peptide-deliver-therapeutic-sirna-and-inhibit-the-invasive-capacity-of-3d-prostate-cancer-tumours
#11
James C Evans, Meenakshi Malhotra, Katrina Sweeney, Raphael Darcy, Colleen C Nelson, Brett G Hollier, Caitriona M O'Driscoll
The main barrier to the development of an effective RNA interference (RNAi) therapy is the lack of a suitable delivery vector. Modified cyclodextrins have emerged in recent years for the delivery of siRNA. In the present study, a folate-targeted amphiphilic cyclodextrin was formulated using DSPE-PEG5000-folate to target prostate cancer cells. The fusogenic peptide GALA was included in the formulation to aid in the endosomal release of siRNA. Targeted nanoparticles were less than 200nm in size with a neutral surface charge...
September 12, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28915356/real-time-apoptosis-and-viability-high-throughput-screening-of-3d-multicellular-tumor-spheroids-using-the-celigo-image-cytometer
#12
Sarah Kessel, Scott Cribbes, Surekha Bonasu, Jean Qiu, Leo Li-Ying Chan
Three-dimensional tumor spheroid models have been increasingly used to investigate and characterize cancer drug compounds. Previously, the Celigo image cytometer has demonstrated its utility in a high-throughput screening manner for evaluating potential drug candidates in a 3D multicellular tumor spheroid (MCTS) primary screen. In addition, we have developed real-time kinetic caspase 3/7 apoptosis and propidium iodide viability 3D MCTS assays, both of which can be used in a secondary screen to better characterize the hit compounds...
September 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28912842/a-reliable-method-for-the-sorting-and-identification-of-aldh-high-cancer-stem-cells-by-flow-cytometry
#13
Zhengwei Leng, Zhao Yang, Lifa Li, Xiaorong Zhong, He Zhou, Yong Li, Gang Yang, Guangjun Zhang, Yongfu Xiong, Tong Zhou, Jianshui Li, Dongsheng Wang, Jingdong Li
Cancer stem cells (CSCs) are a rare tumorigenic population of cells found in multiple types of cancer. It has been suggested that CSCs are responsible for cancer drug resistance, metastasis and recurrence. Therefore, it is important to develop techniques to correctly sort and identify CSCs. In the current study, the sorting and identification of aldehyde dehydrogenase high (ALDH(high)) CSCs was performed using flow cytometry. Cells from three colon cancer cell lines were cultured in serum-free medium to obtain CSCs-enriched spheroid cells...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28912677/reversible-axonal-dystrophy-by-calcium-modulation-in-frataxin-deficient-sensory-neurons-of-yg8r-mice
#14
Belén Mollá, Diana C Muñoz-Lasso, Fátima Riveiro, Arantxa Bolinches-Amorós, Federico V Pallardó, Angel Fernandez-Vilata, María de la Iglesia-Vaya, Francesc Palau, Pilar Gonzalez-Cabo
Friedreich's ataxia (FRDA) is a peripheral neuropathy involving a loss of proprioceptive sensory neurons. Studies of biopsies from patients suggest that axonal dysfunction precedes the death of proprioceptive neurons in a dying-back process. We observed that the deficiency of frataxin in sensory neurons of dorsal root ganglia (DRG) of the YG8R mouse model causes the formation of axonal spheroids which retain dysfunctional mitochondria, shows alterations in the cytoskeleton and it produces impairment of axonal transport and autophagic flux...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28912569/two-dimensional-dirac-particles-in-a-p%C3%A3-schl-teller-waveguide
#15
R R Hartmann, M E Portnoi
We obtain exact solutions to the two-dimensional (2D) Dirac equation for the one-dimensional Pöschl-Teller potential which contains an asymmetry term. The eigenfunctions are expressed in terms of Heun confluent functions, while the eigenvalues are determined via the solutions of a simple transcendental equation. For the symmetric case, the eigenfunctions of the supercritical states are expressed as spheroidal wave functions, and approximate analytical expressions are obtained for the corresponding eigenvalues...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28912559/inherent-aggressive-character-of-invasive-and-non-invasive-cells-dictates-the-in-vitro-migration-pattern-of-multicellular-spheroid
#16
Sukanya Gayan, Abhishek Teli, Tuli Dey
Cellular migration, a process relevant to metastasis, is mostly studied in the conventional 2D condition. However, cells cultured in the 3D condition assumed to mimic the in vivo conditions better. The current study is designed to compare an invasive and non-invasive adenocarcinoma cell with an invasive fibrosarcoma cell to understand the migration pattern of the multicellular spheroid. It is observed that conventional haplotaxis, chemotactic and pseudo-3D migration assay cannot distinguish between the invasive and non-invasive cells conclusively under 2D condition...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28911246/dual-targeting-of-l-carnitine-conjugated-nanoparticles-to-octn2-and-atb-0-to-deliver-chemotherapeutic-agents-for-colon-cancer-therapy
#17
Longfa Kou, Qing Yao, Sathish Sivaprakasam, Qiuhua Luo, Yinghua Sun, Qiang Fu, Zhonggui He, Jin Sun, Vadivel Ganapathy
l-Carnitine, obligatory for oxidation of fatty acids, is transported into cells by the Na(+)-coupled transporter OCTN2 and the Na(+)/Cl(-)-coupled transporter ATB(0,+). Here we investigated the potential of L-carnitine-conjugated poly(lactic-co-glycolic acid) (PLGA) nanoparticles (LC-PLGA NPs) to deliver chemotherapeutic drugs into cancer cells by targeting the nanoparticles to both OCTN2 and ATB(0,+). The cellular uptake of LC-PLGA NPs in the breast cancer cell line MCF7 and the colon cancer cell line Caco-2 was increased compared to unmodified nanoparticles, but decreased in the absence of co-transporting ions (Na(+) and/or Cl(-)) or in the presence of competitive substrates for the two transporters...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28905243/3d-endothelial-cell-spheroid-human-vitreous-humor-assay-for-the-characterization-of-anti-angiogenic-inhibitors-for-the-treatment-of-proliferative-diabetic-retinopathy
#18
Sara Rezzola, Imtiaz M Nawaz, Anna Cancarini, Cosetta Ravelli, Stefano Calza, Francesco Semeraro, Marco Presta
Proliferative diabetic retinopathy (PDR) represents a main cause of acquired blindness. Despite the recognition of the key role exerted by vascular endothelial growth factor (VEGF) in the pathogenesis of PDR, limitations to anti-VEGF therapies do exist. Thus, rapid and cost-effective angiogenesis assays are crucial for the screening of anti-angiogenic drug candidates for PDR therapy. In this context, evaluation of the angiogenic potential of PDR vitreous fluid may represent a valuable tool for preclinical assessment of angiostatic molecules...
September 13, 2017: Angiogenesis
https://www.readbyqxmd.com/read/28904062/optical-coherence-tomography-detects-necrotic-regions-and-volumetrically-quantifies-multicellular-tumor-spheroids
#19
Yongyang Huang, Shunqiang Wang, Qiongyu Guo, Sarah Kessel, Ian Rubinoff, Leo L-Y Chan, Peter Li, Yaling Liu, Jean Qiu, Chao Zhou
Three-dimensional (3D) tumor spheroid models have gained increased recognition as important tools in cancer research and anti-cancer drug development. However, currently available imaging approaches employed in high-throughput screening drug discovery platforms e.g. bright field, phase contrast, and fluorescence microscopies, are unable to resolve 3D structures deep inside (>50 μm) tumor spheroids. In this study, we established a label-free, non-invasive optical coherence tomography (OCT) imaging platform to characterize 3D morphological and physiological information of multicellular tumor spheroids (MCTS) growing from ~250 μm up to ~600 μm in height over 21 days...
September 13, 2017: Cancer Research
https://www.readbyqxmd.com/read/28902902/multiscale-biphasic-modelling-of-peritumoural-collagen-microstructure-the-effect-of-tumour-growth-on-permeability-and-fluid-flow
#20
Peter A Wijeratne, John H Hipwell, David J Hawkes, Triantafyllos Stylianopoulos, Vasileios Vavourakis
We present an in-silico model of avascular poroelastic tumour growth coupled with a multiscale biphasic description of the tumour-host environment. The model is specified to in-vitro data, facilitating biophysically realistic simulations of tumour spheroid growth into a dense collagen hydrogel. We use the model to first confirm that passive mechanical remodelling of collagen fibres at the tumour boundary is driven by solid stress, and not fluid pressure. The model is then used to demonstrate the influence of collagen microstructure on peritumoural permeability and interstitial fluid flow...
2017: PloS One
keyword
keyword
56685
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"