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JOURNAL ARTICLE
Kathleen Patricia Nolan, Daniel Z Grunspan, Erika Myler, Nava Brimble, Andreas Heyland, Robert H Hanner
Course-based undergraduate research experiences (CUREs) increase student access to high impact research experiences. CUREs engage students in the scientific process by learning how to pose scientific questions, develop hypotheses, and generate data to test them. Environmental DNA (eDNA), is a growing field of research that is gaining accessibility through decreasing laboratory costs, which can make a foundation for multiple, engaging CUREs. This manuscript describes three case studies that used eDNA in an upper year undergraduate course...
April 9, 2024: Genome Génome / Conseil National de Recherches Canada
#22
JOURNAL ARTICLE
Josefine Friederike Weiß, Wilken-Jon von Appen, Barbara Niehoff, Nicole Hildebrand, Martin Graeve, Stefan Neuhaus, Astrid Bracher, Eva-Maria Nöthig, Katja Metfies
In Fram Strait, we combined underway-sampling using the remote-controlled Automated Filtration System for Marine Microbes (AUTOFIM) with CTD-sampling for eDNA analyses, and with high-resolution optical measurements in an unprecedented approach to determine variability in plankton composition in response to physical forcing in a sub-mesoscale filament. We determined plankton composition and biomass near the surface with a horizontal resolution of ~ 2 km, and addressed vertical variability at five selected sites...
April 8, 2024: Scientific Reports
#23
JOURNAL ARTICLE
Nestor Naranjo, Inna Florez, Edna Gómez
OBJECTIVE: Understand the social processes experienced by nursing professionals and the meanings underlying autonomy in adult Intensive Care Units in the city of Cartagena (Colombia). METHODS: A qualitative study with a grounded theory approach was conducted. Fifteen semi-structured interviews were carried out with nursing professionals, and the analysis was based on the coding technique proposed by Strauss & Corbin. RESULTS: Of the respondents, fourteen were female and one was male, with ages ranging from 23 to 57 years...
November 2023: Investigación y Educación en Enfermería
#24
JOURNAL ARTICLE
Hans Rund, Josef Wanzenböck, Stefanie Dobrovolny, Rainer Kurmayer
Metabarcoding has been widely accepted as a useful tool for biodiversity assessment based on eDNA. The method allows for the detection of entire groups of organisms in a single sample, making it particularly applicable in aquatic habitats. The high sensitivity of the molecular approaches is especially beneficial in detecting elusive and rare fish species, improving biodiversity assessments. Numerous biotic and abiotic factors that affect the persistence and availability of fish DNA in surface waters and therefore affecting species detectability, have been identified...
April 6, 2024: Science of the Total Environment
#25
JOURNAL ARTICLE
Itzel Jatziri Contreras-García, Cindy Bandala, Iván Ignacio-Mejía, Luz Adriana Pichardo-Macías, Julieta Griselda Mendoza-Torreblanca, Mercedes Edna García-Cruz, Saúl Gómez-Manzo, Noemí Cárdenas-Rodríguez
Levetiracetam (LEV) is a drug commonly used as an anticonvulsant. However, recent evidence points to a possible role as an antioxidant. We previously demonstrated the antioxidant properties of LEV by significantly increasing catalase and superoxide dismutase activities and decreasing the hydrogen peroxide (H2O2) levels in the hippocampus of rats with temporal lobe epilepsy (TLE) showing scavenging properties against the hydroxyl radical. The aim of the present work was to evaluate, the effect of LEV on DNA oxidation, by determining 8‑hydroxy‑2‑deoxyguanosine (8‑OHdG) levels, and glutathione content, through reduced (GSH) and oxidized (GSSG) glutathione levels, in the hippocampus of rats with TLE...
March 28, 2024: Acta Neurobiologiae Experimentalis
#26
JOURNAL ARTICLE
Nina Filippova, Elena Zvyagina, Elena A Rudykina, Tagir F Ishmanov, Ilya V Filippov, Tatiana M Bulyonkova, Alevtina S Dobrynina
BACKGROUND: The paper represents the first DNA-based occurrence dataset on peatland fungal communities published for north-western Siberia, the first for Russia and complements several existing datasets on metabarcoding of peat soils globally. NEW INFORMATION: The aim of the present publication is to describe the first DNA-based occurrence dataset on fungal communities in peat soils and other substrates studied by the eDNA approach in the Mukhrino raised bog, located in a large paludified area of north-western Siberia...
2024: Biodiversity Data Journal
#27
JOURNAL ARTICLE
Zongwu Wei, Xuzhe Zhang, Yingzhan Chen, Hongjie Liu, Shaopeng Wang, Man Zhang, Honglin Ma, Kefu Yu, Liwei Wang
Population outbreaks of the crown-of-thorns starfish (COTS) seriously threaten the sustainability of coral reef ecosystems. However, traditional ecological monitoring techniques cannot provide early warning before the outbreaks, thus preventing timely intervention. Therefore, there is an urgent need for a more accurate and faster technology to predict the outbreaks of COTS. In this work, we developed an electrochemical biosensor based on a programmed catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) cyclic amplification strategy for sensitive and selective detection of COTS environmental DNA (eDNA) in water bodies...
April 5, 2024: Science of the Total Environment
#28
JOURNAL ARTICLE
Maximilian Knoll, Lorenz Gygax, Edna Hillmann
Accelerometers are useful in analyzing lying behavior in farm animals. The effect of the farrowing system on sow lying behavior has been studied around parturition, but not long-term. In a natural environment, sows increase activity 14 days post parturition, which we expected to be also evident in housed sows when they can move freely. The objective of this study was i) to validate the methodology to automatically measure sow lying bouts and duration with accelerometers and ii) to apply it to crated and free farrowing sows 24 hours pre-parturition until weaning...
April 6, 2024: Journal of Animal Science
#29
JOURNAL ARTICLE
Silvia Fernández-García, Laura Del Campo-Albendea, Dharshini Sambamoorthi, Jameela Sheikh, Karen Lau, Nana Osei-Lah, Anoushka Ramkumar, Harshitha Naidu, Nicole Stoney, Paul Sundaram, Paulomi Sengupta, Samay Mehta, Shruti Attarde, Sophie Maddock, Millie Manning, Zainita Meherally, Kehkashan Ansari, Heidi Lawson, Magnus Yap, Tania Kew, Andriya Punnoose, Chloe Knight, Eyna Sadeqa, Jiya Cherian, Sangamithra Ravi, Wentin Chen, Kate Walker, Keelin O'Donoghue, Madelon van Wely, Elizabeth van Leeuwen, Elena Kostova, Heinke Kunst, Asma Khalil, Vanessa Brizuela, Edna Kara, Caron Rahn Kim, Anna Thorson, Olufemi T Oladapo, Lynne Mofenson, Sami L Gottlieb, Mercedes Bonet, Ngawai Moss, Javier Zamora, John Allotey, Shakila Thangaratinam
OBJECTIVE: To assess the effects of COVID-19 vaccines in women before or during pregnancy on SARS-CoV-2 infection-related, pregnancy, offspring and reactogenicity outcomes. DESIGN: Systematic review and meta-analysis. DATA SOURCES: Major databases between December 2019 and January 2023. STUDY SELECTION: Nine pairs of reviewers contributed to study selection. We included test-negative designs, comparative cohorts and randomised trials on effects of COVID-19 vaccines on infection-related and pregnancy outcomes...
April 4, 2024: BMJ Global Health
#30
JOURNAL ARTICLE
Stephanie Novoa-Sanzana, José Moya-Osorio, Yadira Morejón Terán, Israel Ríos-Castillo, Luis Miguel Becerra Granados, Gloria Prada Gómez, Mónica Ramos de Ixtacuy, Roxana Carla Fernández Condori, María Celeste Nessier, Ana Guerrero Gómez, Laura González-Céspedes, Edna J Nava-González, Luisa Pérez Ocampo, Adriana Nathaly Castillo-Albarracín, Samuel Durán-Agüero
OBJECTIVE: To understand the association of food insecurity with sociodemographic factors in a sample population in Latin America during the COVID-19 pandemic. METHODS: This was a multicenter cross-sectional study conducted in 10 countries in Latin America using an online survey through various digital platforms from October 14, 2020 to February 15, 2021. Statistical analysis of data was performed by applying descriptive statistics, chi-square test, and logistic regression analysis...
2024: Pan American Journal of Public Health
#31
JOURNAL ARTICLE
Pedro Paulo da Costa Alves Filho, Wendel Valter da Silveira Pereira, Yan Nunes Dias, Adriele Laena Ferreira de Moraes, Flavio Henrique Santos Rodrigues, Sílvio Junio Ramos, Edna Santos de Souza, Anna Maria Fontes do Amaral, Antonio Rodrigues Fernandes
Artisanal mining is intensely carried out in developing countries, including Brazil and especially in the Amazon. This method of mineral exploration generally does not employ mitigation techniques for potential damages and can lead to various environmental problems and risks to human health. The objectives of this study were to quantify the concentrations of rare earth elements (REEs) and estimate the environmental and human health risks in cassiterite and monazite artisanal mining areas in the southeastern Amazon, as well as to understand the dynamics of this risk over time after exploitation...
April 4, 2024: Environmental Management
#32
JOURNAL ARTICLE
Shuping Wang, Songsong Gu, Yaqun Zhang, Ye Deng, Wenhui Qiu, Qianhang Sun, Tianxu Zhang, Pengyuan Wang, Zhenguang Yan
Ecological water replenishment (EWR) is an important strategy for river restoration globally, but timely evaluation of its ecological effects at a large spatiotemporal scale to further adjust the EWR schemes is of great challenge. Here, we examine the impact of EWR on microeukaryotic plankton communities in three distinct river ecosystems through environmental DNA (eDNA) metabarcoding. The three ecosystems include a long-term cut-off river, a short-term connected river after EWR, and long-term connected rivers...
July 2024: Environ Sci Ecotechnol
#33
JOURNAL ARTICLE
Johanna Honka, Laura Kvist, Suvi Olli, Toni Laaksonen, Jouni Aspi
For many aquatic and semiaquatic mammal, amphibian and fish species, environmental DNA (eDNA) methods are employed to detect species distribution and to monitor their presence, but eDNA is much less employed for avian species. Here, we developed primers for the detection of true geese and swan species using eDNA and optimised a PCR protocol for eDNA. We selected taiga bean goose ( Anser fabalis fabalis ) as our focal (sub)species and sampled water from lakes, from which the presence of taiga bean goose was visually confirmed...
April 2024: Ecology and Evolution
#34
Mariam Almutairi, Katherine Chechalk, Emelia Deane, Rebecca Fox, Ava Janes, Tidgh Maguire-Henry, Devin McCabe, Cole O'Connor, Joseph Quirk, Evan Swan, Katherine White, Kathryn McCreery, Eman Isweisi, Philip Stewart, Aoife Branagan, Edna F Roche, Judith Meehan, Eleanor J Molloy
AIM: Retinopathy of prematurity is a significant global cause of childhood blindness. This study aims to identify serum biomarkers that are associated with the development of ROP. METHODS: A systematic review and meta-analysis was conducted using PRISMA guidelines. Three databases were searched (Pubmed, Scopus and Web of Science) from 2003 to March 2023. Only studies investigating serum biomarker levels in preterm infants (<37 weeks gestation) were included. RESULTS: Meta-analysis suggests that low serum IGF-1 levels have a strong association with the development of ROP [SMD (95% CI) of -...
2024: Frontiers in Pediatrics
#35
JOURNAL ARTICLE
Wanhe Luo, Yongtao Jiang, Jinhuan Liu, Beibei Sun, Xiuge Gao, Samah Attia Algharib, Dawei Guo, Jie Wei, Yurong Wei
BACKGROUND: Biofilms, such as those from Staphylococcus epidermidis , are generally insensitive to traditional antimicrobial agents, making it difficult to inhibit their formation. Although quercetin has excellent antibiofilm effects, its clinical applications are limited by the lack of sustained and targeted release at the site of S. epidermidis infection. OBJECTIVES: Polyethylene glycol-quercetin nanoparticles (PQ-NPs)-loaded gelatin-N,O-carboxymethyl chitosan (N,O-CMCS) composite nanogels were prepared and assessed for the on-demand release potential for reducing S...
March 2024: Journal of Veterinary Science
#36
JOURNAL ARTICLE
Vitor de Toledo Stuani, Matheus Dante de Paula, Raphaella Coelho Michel, Gustavo Gonçalves do Prado Manfredi, Edna Maria de Oliveira Fernandes, Diana Gabriela Soares Dos Passos
OBJECTIVE: This study evaluated the accuracy and precision of digital models acquisition using a home-built, low-cost scanning system based on the structured light method. METHODS: a plaster model (PM) was scanned using the experimental device (SL) and a dental desktop scanner (DS). The teeth dimensions of PM and SL models were measured in triplicate, with a caliper and digitally, respectively. The agreement of the measurements of each model was evaluated using the intraclass correlation coefficient, and the validity between the different measurement techniques was assessed using the Bland-Altman analysis...
2024: Dental Press Journal of Orthodontics
#37
JOURNAL ARTICLE
Shruti Shah, Samir R Damare, Maria Brenda Luzia Mascarenhas-Pereira, Jayesh Patil, Sneha Parab, Sushil Nair, Arpita Ghosh
Deep sea is a vast, dark, and difficult-to-access terrain and is now looked upon as a unique niche harboring diverse microorganism. We used a metataxonomic approach to decipher the microbial diversity present in the water column (surface to near bottom), water overlaying the sediments, and the deep-sea sediments (up to 35 cm) from the Indian Contract Region (ICR) in the Central Indian Ocean Basin (CIOB). Samples were collected from #IRZ (Impact Reference Zone), #PRZ (Potential Reference Zone), and #BC20 (Control site, outside potential mining area) with an average water depth of 5,200 m...
2024: Frontiers in Microbiology
#38
JOURNAL ARTICLE
Khanathip Jitpakdee, Fabian Sommer, Edna Gouveia, Catherine Mykolajtchuk, Blake Boadi, Jessica Berger, Ibrahim Hussain, Roger Härtl
BACKGROUND: Failure to restore lordotic alignment is not an uncommon problem following minimally invasive transforaminal lumbar interbody fusion (MIS TLIF), even with expandable cages that increase disc height. This study aims to investigate the effect of the expandable cage that is specifically designed to expand both height and lordosis. We evaluated the outcomes of MIS TLIF in restoring immediate postoperative sagittal alignment by comparing two different types of expandable cages...
March 20, 2024: Journal of Spine Surgery (Hong Kong)
#39
JOURNAL ARTICLE
Histological and molecular difference in albumen quality between post-adolescent hens and aged hens.
Xin-Yu Chang, Obianwuna Uchechukwu Edna, Jing Wang, Hai-Jun Zhang, Jian-Min Zhou, Kai Qiu, Shu-Geng Wu
The decline in albumen quality resulting from aging hens poses a threat to the financial benefits of the egg industry. Exploring the underlying mechanisms from the perspective of cell molecules of albumen formation is significant for the efficient regulation of albumen quality. Two individual groups of Hy-Line Brown layers with ages of 40 (W40) and 100 (W100) wk old were used in the present study. Each group contained over 2,000 birds. This study assessed the egg quality, biochemical indicators and physiological status of hens between W40 and W100...
March 6, 2024: Poultry Science
#40
JOURNAL ARTICLE
Steffen Swoboda, Thomas Krumpen, Eva-Maria Nöthig, Katja Metfies, Simon Ramondenc, Jutta Wollenburg, Kirsten Fahl, Ilka Peeken, Morten Iversen
Globally, the most intense uptake of anthropogenic carbon dioxide (CO2 ) occurs in the Atlantic north of 50°N, and it has been predicted that atmospheric CO2 sequestration in the Arctic Ocean will increase as a result of ice-melt and increased primary production. However, little is known about the impact of pan-Arctic sea-ice decline on carbon export processes. We investigated the potential ballasting effect of sea-ice derived material on settling aggregates and carbon export in the Fram Strait by combining 13 years of vertical flux measurements with benthic eDNA analysis, laboratory experiments, and tracked sea-ice distributions...
April 2024: PNAS Nexus
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