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https://www.readbyqxmd.com/read/28428746/expression-of-d-amino-acid-oxidase-dao-daao-and-d-amino-acid-oxidase-activator-daoa-g72-during-development-and-aging-in-the-human-post-mortem-brain
#1
Vinita Jagannath, Zoya Marinova, Camelia-Maria Monoranu, Susanne Walitza, Edna Grünblatt
In the brain, D-amino acid oxidase (DAO/DAAO) mainly oxidizes D-serine, a co-agonist of the N-methyl-D-aspartate (NMDA) receptors. Thus, DAO can regulate the function of NMDA receptors via D-serine breakdown. Furthermore, DAO activator (DAOA)/G72 has been reported as both DAOA and repressor. The co-expression of DAO and DAOA genes and proteins in the human brain is not yet elucidated. The aim of this study was to understand the regional and age span distribution of DAO and DAOA (mRNA and protein) in a concomitant manner...
2017: Frontiers in Neuroanatomy
https://www.readbyqxmd.com/read/28426246/biofilm-characteristics-and-evaluation-of-the-sanitation-procedures-of-thermophilic-aeribacillus-pallidus-e334-biofilms
#2
Tugba Kilic, Basar Karaca, Beste Piril Ozel, Birgul Ozcan, Cumhur Cokmus, Arzu Coleri Cihan
The ability of Aeribacillus pallidus E334 to produce pellicle and form a biofilm was studied. Optimal biofilm formation occurred at 60 °C, pH 7.5 and 1.5% NaCl. Extra polymeric substances (EPS) were composed of proteins and eDNA (21.4 kb). E334 formed biofilm on many surfaces, but mostly preferred polypropylene and glass. Using CLSM analysis, the network-like structure of the EPS was observed. The A. pallidus biofilm had a novel eDNA content. DNaseI susceptibility (86.8% removal) of eDNA revealed its importance in mature biofilms, but the purified eDNA was resistant to DNaseI, probably due to its extended folding outside the matrix...
April 20, 2017: Biofouling
https://www.readbyqxmd.com/read/28424111/management-of-recurrent-invasive-ductal-carcinoma-in-transrectus-abdominis-myocutaneous-flap-and-lymphatic-remodeling-to-the-contralateral-axilla
#3
Eric Chung, Edna Kapenhas, Dan Badia, Max Spektor, Bradley S Gluck, Sharon Secola
No abstract text is available yet for this article.
April 1, 2017: American Surgeon
https://www.readbyqxmd.com/read/28423113/time-trends-for-tobacco-and-alcohol-use-in-youth-rated-films-popular-in-mexico-and-argentina-from-2004-2012
#4
Inti Barrientos-Gutiérrez, Raul Mejía, Rosaura Pérez-Hernández, Christy Kollath-Cattano, Lorena Peña, Paola Morello, Edna Arillo-Santillán, Sandra Braun, James D Sargent, James F Thrasher
Objective: To examine and compare overall prevalence and time trends in tobacco and alcohol portrayals and brand appearances in youth-rated US and nationally-produced films that were the most successful in Argentina and Mexico from 2004-2012. Materials and methods: Top-grossing nationally produced films from Argentina (n=73), Mexico (n=85) and the US (n=643) were content analyzed. Logistic regression was used to determine differences between Mexican, Argentine and US produced films...
January 2017: Salud Pública de México
https://www.readbyqxmd.com/read/28420209/association-between-blood-lead-levels-and-delta-aminolevulinic-acid-dehydratase-in-pregnant-women
#5
Osmel La-Llave-León, Edna M Méndez-Hernández, Francisco X Castellanos-Juárez, Eloísa Esquivel-Rodríguez, Fernando Vázquez-Alaniz, Ada Sandoval-Carrillo, Gonzalo García-Vargas, Jaime Duarte-Sustaita, Jorge L Candelas-Rangel, José M Salas-Pacheco
Blood lead levels (BLLs) and delta-aminolevulinic acid dehydratase (ALAD) activity are considered biomarkers of lead exposure and lead toxicity, respectively. The present study was designed to investigate the association between BLLs and ALAD activity in pregnant women from Durango, Mexico. A total of 633 pregnant women aged 13-43 years participated in this study. Blood lead was measured by a graphite furnace atomic absorption spectrometer. ALAD activity was measured spectrophotometrically. Mean blood lead was 2...
April 18, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28419268/the-american-society-for-clinical-pathology-s-2015-wage-survey-of-medical-laboratories-in-the-united-states
#6
Edna Garcia, Patrick B Fisher
Objectives: To inform the pathology and laboratory field of the most recent national wage data from the American Society for Clinical Pathology (ASCP). Historically, the results of this biennial survey have served as a basis for additional research on laboratory recruitment, retention, education, marketing, certification, and advocacy. Methods: The 2015 wage survey was conducted through collaboration between the ASCP's Institute of Science, Technology, and Policy in Washington, DC, and the ASCP Board of Certification in Chicago, Illinois...
April 15, 2017: Laboratory Medicine
https://www.readbyqxmd.com/read/28414038/modulation-of-staphylococcus-epidermidis-rp62a-extracellular-polymeric-layer-by-marine-cyclic-dipeptide-cyclo-l-leucyl-l-prolyl-thwarts-biofilm-formation
#7
Shanmugaraj Gowrishankar, Shunmugiah Karutha Pandian
Staphylococcus epidermidis is the most frequent cause of biofilm mediated implant-associated infections. Extracellular polymeric substance (EPS) is a key component of most biofilms and in pathogens it specifically protects the entrenched-bacterial cells from antibiotics and hosts immune response, and thereby makes the infection ineradicable. Recently, the prominence of cyclic dipeptides in interfering with biofilms and the associated virulence factors of pathogens has offered an alternative to eliminate difficult-to-treat infections...
April 14, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28403348/neonatal-necrotizing-enterocolitis-rat-model-attenuated-by-a-remote-ischemic-preconditioning-in-the-pregnant
#8
Rúdnei de Oliveira Luciano Gomes, Ricardo Artigiani, José de Freitas Guimarães, Adriana Porto Nunes, Edna Frasson de Souza Montero, José Luiz Martins
Purpose: To evaluate the effect of remote ischemic preconditioning (r-IPC) administered to pregnant rats, in the ileum of newborn rats subjected to hypoxia and reoxygenation. Methods: We used three pregnant rats and their newborn rats distributed in three groups: 1) Control (C) - Newborn rats born from a pregnant rat which did not undergo any intervention; 2) Hypoxia-Reoxygenation (H/R) - Newborn rats born from a pregnant rat which did not undergo any intervention, and were subjected to hypoxia-reoxygenation; 3) Remote Ischemic Preconditioning (r-IPC) - newborn rats born from a pregnant rat which was subjected to remote ischemic preconditioning twenty-four hours before giving birth and the newborn rats were subjected to hypoxia-reoxygenation...
March 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28403183/aquatic-environmental-dna-detects-seasonal-fish-abundance-and-habitat-preference-in-an-urban-estuary
#9
Mark Y Stoeckle, Lyubov Soboleva, Zachary Charlop-Powers
The difficulty of censusing marine animal populations hampers effective ocean management. Analyzing water for DNA traces shed by organisms may aid assessment. Here we tested aquatic environmental DNA (eDNA) as an indicator of fish presence in the lower Hudson River estuary. A checklist of local marine fish and their relative abundance was prepared by compiling 12 traditional surveys conducted between 1988-2015. To improve eDNA identification success, 31 specimens representing 18 marine fish species were sequenced for two mitochondrial gene regions, boosting coverage of the 12S eDNA target sequence to 80% of local taxa...
2017: PloS One
https://www.readbyqxmd.com/read/28397645/flattening-and-unpacking-human-genetic-variation-in-mexico-postwar-to-present
#10
Víctor Hugo Anaya-Muñoz, Vivette García-Deister, Edna Suárez-Díaz
Argument This paper analyzes the research strategies of three different cases in the study of human genetics in Mexico - the work of Rubén Lisker in the 1960s, INMEGEN's mapping of Mexican genomic diversity between 2004 and 2009, and the analysis of Native American variation by Andrés Moreno and his colleagues in contemporary research. We make a distinction between an approach that incorporates multiple disciplinary resources into sampling design and interpretation (unpacking), from one that privileges pragmatic considerations over more robust multidisciplinary analysis (flattening)...
March 2017: Science in Context
https://www.readbyqxmd.com/read/28393885/seasonal-variation-in-environmental-dna-in-relation-to-population-size-and-environmental-factors
#11
Andrew S Buxton, Jim J Groombridge, Nurulhuda B Zakaria, Richard A Griffiths
Analysing DNA that organisms release into the environment (environmental DNA, or eDNA) has enormous potential for assessing rare and cryptic species. At present the method is only reliably used to assess the presence-absence of species in natural environments, as seasonal influences on eDNA in relation to presence, abundance, life stages and seasonal behaviours are poorly understood. A naturally colonised, replicated pond system was used to show how seasonal changes in eDNA were influenced by abundance of adults and larvae of great crested newts (Triturus cristatus)...
April 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28392261/bound-waters-mediate-binding-of-diverse-substrates-to-a-ubiquitin-ligase
#12
Juliana Muñoz-Escobar, Edna Matta-Camacho, Cordelia Cho, Guennadi Kozlov, Kalle Gehring
The N-end rule pathway controls the half-life of proteins based on their N-terminal residue. Positively charged type 1 N-degrons are recognized by a negatively charged pocket on the Zn finger named the UBR box. Here, we show that the UBR box is rigid, but bound water molecules in the pocket provide the structural plasticity required to bind different positively charged amino acids. Ultra-high-resolution crystal structures of arginine, histidine, and methylated arginine reveal that water molecules mediate the binding of N-degron peptides...
March 27, 2017: Structure
https://www.readbyqxmd.com/read/28383750/using-quantitative-literacy-to-enhance-critical-thinking-skills-in-undergraduate-nursing-students
#13
Edna Asknes
BACKGROUND: Critical thinking and quantitative literacy (QL) are similarly grounded: both focus on analyzing and evaluating evidence, identifying implications and consequences, drawing inferences, and communicating information. This teaching strategy was based on those commonalities and was designed so that undergraduate nursing students would enhance their critical thinking skills as they used their QL skills. METHOD: QL skills are most effective when taught, learned, and used to solve significant, pertinent problems...
April 1, 2017: Journal of Nursing Education
https://www.readbyqxmd.com/read/28364806/lufenuron-impact-upon-anthonomus-grandis-boheman-coleoptera-curculionidae-midgut-and-its-reflection-in-gametogenesis
#14
Hilton Nobre Costa, Franklin Magliano da Cunha, Glaucilane Santos Cruz, Carolline Guimarães D'assunção, Guilherme Gomes Rolim, Maria Edna Gomes Barros, Mariana Oliveira Breda, Alvaro Aguiar Coelho Teixeira, Valéria Wanderley Teixeira
The insecticide Match® (lufenuron), one of the main insect growth regulators used in pest control, has been presented as a viable alternative against the boll weevil, Anthonomus grandis (Coleoptera: Curculionidae), by inhibiting chitin synthesis. Thus, this study aimed to examine whether Match® interferes in the synthesis of the peritrophic matrix, leading to changes in the midgut epithelium, resulting in nutritional deficiency and reflecting, thereby, in the gametogenesis process of A. grandis. Floral cotton buds were immersed in the insecticide solution (800μL of Match®+200mL of distilled water) and offered to the adult insects...
April 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/28357478/application-of-environmental-dna-analysis-to-inform-invasive-fish-eradication-operations
#15
Phillip I Davison, Gordon H Copp, Véronique Créach, Lorenzo Vilizzi, J R Britton
Environmental DNA (eDNA) detection of non-native species has considerable potential to inform management decisions, including identifying the need for population control and/or eradication. An invasive species of European concern is the Asian cyprinid fish, topmouth gudgeon (Pseudorasbora parva). Here, eDNA analyses were applied at a commercial angling venue in southern England to inform operations aiming to eradicate P. parva, which had only ever been observed in one of the venue's seven unconnected angling ponds...
April 2017: Die Naturwissenschaften
https://www.readbyqxmd.com/read/28355216/chirosurveillance-the-use-of-native-bats-to-detect-invasive-agricultural-pests
#16
Brooke Maslo, Rafael Valentin, Karen Leu, Kathleen Kerwin, George C Hamilton, Amanda Bevan, Nina H Fefferman, Dina M Fonseca
Invasive insect pests cost the agricultural industry billions of dollars annually in crop losses. Timely detection of pests is critical for management efficiency. Innovative pest detection strategies, such as environmental DNA (eDNA) techniques, combined with efficient predators, maximize sampling resolution across space and time and may improve surveillance. We tested the hypothesis that temperate insectivorous bats can be important sentinels of agricultural insect pest surveillance. Specifically, we used a new high-sensitivity molecular assay for invasive brown marmorated stink bugs (Halyomorpha halys) to examine the extent to which big brown bats (Eptesicus fuscus) detect agricultural pests in the landscape...
2017: PloS One
https://www.readbyqxmd.com/read/28353120/the-molecular-basis-of-evolution-and-disease-a-cold-war-alliance
#17
Edna Suárez-Díaz
This paper extends previous arguments against the assumption that the study of variation at the molecular level was instigated with a view to solving an internal conflict between the balance and classical schools of population genetics. It does so by focusing on the intersection of basic research in protein chemistry and the molecular approach to disease with the enactment of global health campaigns during the Cold War period. The paper connects advances in research on protein structure and function as reflected in Christian Anfinsen´s The molecular basis of evolution, with a political reading of Emilé Zuckerkandl and Linus Pauling's identification of molecular disease and evolution...
March 28, 2017: Journal of the History of Biology
https://www.readbyqxmd.com/read/28348602/incidence-and-predictors-of-antiretroviral-treatment-modification-in-hiv-infected-adults-a-brazilian-historical-cohort-from-2001-to-2010
#18
Letícia Penna Braga, Cássia Cristina Pinto Mendicino, Edna Afonso Reis, Ricardo Andrade Carmo, Cristiane Menezes de Pádua
This study estimated the incidence of and time to first antiretroviral therapy (ART) modification. This longitudinal analysis comprised a sample of 236 patients from three HIV/AIDS referral centers in Belo Horizonte, Brazil-part of a major historical cohort. Inclusion criteria were as follows: having been treatment-naive patient ≥18 years old who initiated ART between 2001 and 2005 in these three referral centers. The main endpoint was time to first ART modification. Patients were followed up for five years, covering the period 2001-2010, during which time Pearson's chi-square test was performed to compare ART modification between groups...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28345929/utility-of-cyp3a4-and-pxr-car-cyp3a4-3a7-transgenic-mouse-models-to-assess-the-magnitude-of-cyp3a4-mediated-drug-drug-interactions
#19
Justin Q Ly, Kirsten Messick, Ann Qin, Ryan H Takahashi, Edna F Choo
Species differences in the expression, activity, regulation, and substrate specificity of metabolizing enzymes preclude the use of animal models to predict clinical drug-drug interactions (DDIs). The objective of this work is to determine if the transgenic (Tg) Cyp3a(-/-)Tg-3A4Hep/Int and Nr1i2/Nr1i3(-/-)-Cyp3a(-/-)Tg-PXR-CAR-3A4/3A7Hep/Int (PXR-CAR-CYP3A4/3A7) mouse models could be used to predict in vivo DDI of 10 drugs; alprazolam, bosutinib, crizotinib, dasatinib, gefitinib, ibrutinib, regorafenib, sorafenib, triazolam, and vandetinib (as victims); with varying magnitudes of reported CYP3A4 clinical DDI...
April 7, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28345609/environmental-dna-in-subterranean-biology-range-extension-and-taxonomic-implications-for-proteus
#20
Špela Gorički, David Stanković, Aleš Snoj, Matjaž Kuntner, William R Jeffery, Peter Trontelj, Miloš Pavićević, Zlatko Grizelj, Magdalena Năpăruş-Aljančič, Gregor Aljančič
Europe's obligate cave-dwelling amphibian Proteus anguinus inhabits subterranean waters of the north-western Balkan Peninsula. Because only fragments of its habitat are accessible to humans, this endangered salamander's exact distribution has been difficult to establish. Here we introduce a quantitative real time polymerase chain reaction-based environmental DNA (eDNA) approach to detect the presence of Proteus using water samples collected from karst springs, wells or caves. In a survey conducted along the southern limit of its known range, we established a likely presence of Proteus at seven new sites, extending its range to Montenegro...
March 27, 2017: Scientific Reports
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