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Roffi Grandiosa, Mai-Louise Bouwman, Tim Young, Fabrice Mérien, Andrea C Alfaro
The ability to successfully prepare and preserve haemocyte cells for microscopy and flow cytometry is critical for the investigation of animal immune systems. In this study, we observed the total cell count, in vitro viability and stability of New Zealand black-footed abalone (Haliotis iris) haemocytes with different antiaggregants and handling protocols. Haemocyte stability was evaluated by direct observation of haemocytes under the microscope and calculating the aggregation index. Haemocyte counts and viability were measured via flow cytometry and tested for the effect of different antiaggregants (Alsever's solution at three concentrations, and specialised blood collection tubes containing lithium heparin and K2 EDTA) at different temperatures and storage times...
April 20, 2018: Fish & Shellfish Immunology
Reham M Dawood, Mai Abd El-Meguid, Marwa K Ibrahim, Noha G Bader El Din, Ahmed Barakat, Khaled El-Wakeel, Mohamed Darwish Ahmed Abd Alla, George Y Wu, Mostafa K El Awady
BACKGROUND: Liver fibrosis results from a wound healing response to chronic injury, which leads to excessive matrix deposition. Genome wide association studies have showen transcriptional dysregulation in mild and severe liver fibrosis. Recent studies suggested that genetic markers may be able to define the exact stage of liver fibrosis. AIM: To define genes or genetic pathways that could serve as markers for staging or as therapeutic targets to halt progression of liver fibrosis...
April 20, 2018: Gene
Kiyoshi Yasui, Mai Izumida, Takeya Nakagawa, Yoshinao Kubo, Hideki Hayashi, Takashi Ito, Hiroaki Ikeda, Toshifumi Matsuyama
Interferon regulatory factor (IRF) 4 and the proto-oncogene c-Rel cooperate in growth and antiviral drug resistance of adult T-cell leukemia/lymphoma (ATLL). To elucidate the target of IRF4 and c-Rel in ATLL, we determined the simultaneous binding sites of IRF4 and c-Rel using ChIP-seq technology. Nine genes were identified within 2 kb of binding sites, including MIR3662. Expression of miR-3662 was regulated by IRF4, and to a lesser extent by c-Rel. Cell proliferation was inhibited by knockdown of miR-3662 and expression of miR-3662 was correlated with antiviral drug resistance in ATLL cell lines...
April 20, 2018: Biochemical and Biophysical Research Communications
HaiTao Liu, Yin Tao, Mai Chen, Jin Yu, Wei-Jie Li, Ling Tao, Yan Li, Fei Li
BACKGROUND The formation of new blood vessels, known as angiogenesis, is critical for recovery from ischemic heart disease, and estrogen is considered an important factor in this process. Here, we investigated the effects of 17β-estradiol (17β-E2) on proliferation and migration of cardiac microvascular endothelial cells (CMECs) in vitro. MATERIAL AND METHODS Rat CMECs were isolated and cultured with 17β-E2 (0.001-1 µmol/l) in the absence or presence of the estrogen antagonist tamoxifen. Then, the expression level of estrogen receptor alpha was evaluated by using immunofluorescence assay, RT-PCR, and Western blot...
April 23, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Suchitporn Lersilp, Supawadee Putthinoi, Sayaka Okahashi
Background: Assistive technology (AT) is a way to enhance the performance of daily activities in people with disabilities and help them live more independently. However, an important problem in providing AT lies in the effectiveness of information management. Local policymakers and health service providers have become aware of this problem and their perspectives are the key to solving it. Methods: This study explored the types of AT provided for people with disabilities in the community and the perspectives on information management of local policymakers and health service providers...
2018: Occupational Therapy International
Luc Djoussé, Yuk-Lam Ho, Xuan-Mai T Nguyen, David R Gagnon, Peter W F Wilson, Kelly Cho, J Michael Gaziano
BACKGROUND: While adherence to healthful dietary patterns has been associated with a lower risk of coronary artery disease (CAD) in the general population, limited data are available among US veterans. We tested the hypothesis that adherence to Dietary Approach to Stop Hypertension (DASH) food pattern is associated with a lower risk of developing CAD among veterans. METHODS AND RESULTS: We analyzed data on 153 802 participants of the Million Veteran Program enrolled between 2011 and 2016...
April 21, 2018: Journal of the American Heart Association
K Pérez, W Weijermars, N Bos, A J Filtness, R Bauer, H Johannsen, N Nuyttens, L Pascal, P Thomas, M Olabarria
To determine accurately the number of serious injuries at EU level and to compare serious injury rates between different countries it is essential to use a common definition. In January 2013, the High Level Group on Road Safety established the definition of serious injuries as patients with an injury level of MAIS3+(Maximum Abbreviated Injury Scale). Whatever the method used for estimating the number or serious injuries, at some point it is always necessary to use hospital records. The aim of this paper is to understand the implications for (1) in/exclusion criteria applied to case selection and (2) a methodological approach for converting ICD (International Classification of Diseases/Injuries) to MAIS codes, when estimating the number of road traffic serious injuries from hospital data...
April 18, 2018: Accident; Analysis and Prevention
Toshiyuki Ishiba, Andreas-Claudius Hoffmann, Joshua Usher, Yahya Elshimali, Todd Sturdevant, Mai Dang, Yolanda Jaimes, Rama Tyagi, Ronald Gonzalez, Mary Grino, Jacek K Pinski, Afsaneh Barzi, Luis E Raez, Wilfried E Eberhardt, Dirk Theegarten, Heinz-Josef Lenz, Hiroyuki Uetake, Peter V Danenberg, Kathleen Danenberg
BACKGROUND: Precision medicine and prediction of therapeutic response requires monitoring potential biomarkers before and after treatment. Liquid biopsies provide noninvasive prognostic markers such as circulating tumor DNA and RNA. Circulating tumor RNA (ctRNA) in blood is also used to identify mutations in genes of interest, but additionally, provides information about relative expression levels of important genes. In this study, we analyzed PD-L1 expression in ctRNA isolated from various cancer types...
April 18, 2018: Biochemical and Biophysical Research Communications
Yang Mai, Liu Dou, Sudaxshina Murdan, Abdul Basit
There is a growing body of evidence which suggests that formerly regarded "inert" pharmaceutical excipients have the potential to influence oral drug bioavailability. The solubilizing agent polyethylene glycol 400 (PEG 400), for instance, has a sex-specific effect on P-glycoprotein (P-gp)-mediated drug bioavailability. We hypothesized that such an effect could be via PEG-induced alteration of P-gp activity and/or expression to different extents in males and females. To test this hypothesis in vivo, we investigated the influence of orally administered PEG 400 on the protein content and mRNA expression of P-gp in different regions of the gastrointestinal tract in male and female rats...
April 17, 2018: European Journal of Pharmaceutical Sciences
Mai-Britt Worm Ørntoft
Colorectal cancer (CRC) is a leading cause of cancer related deaths worldwide, and late stages (III-IV) in particular have low 5-year survival rates. Stage shifting by CRC screening programs has proven effective by decreasing morbidity and mortality and in many countries national CRC screening programs have been implemented. Currently, European, Asian, and American authorities recommend screening for CRC using fecal occult blood testing, sigmoidoscopy, or colonoscopy. Because these approaches all have weaknesses (eg, poor compliance, high costs, test invasiveness), much effort has been put into the development of alternative screening approaches, many of which are blood-based...
March 2, 2018: Clinical Colorectal Cancer
Steven D Fidanze, Dachun Liu, Robert A Mantei, Lisa A Hasvold, John K Pratt, George S Sheppard, Le Wang, James H Holms, Yujia Dai, Ana Aguirre, Andrew Bogdan, Justin D Dietrich, Jasmina Marjanovic, Chang H Park, Charles W Hutchins, Xiaoyu Lin, Mai H Bui, Xiaoli Huang, Denise Wilcox, Leiming Li, Rongqi Wang, Peter Kovar, Terrance J Magoc, Ganesh Rajaraman, Daniel H Albert, Yu Shen, Warren M Kati, Keith F McDaniel
Novel conformationally constrained BET bromodomain inhibitors have been developed. These inhibitors were optimized in two similar, yet distinct chemical series, the 6-methyl-1H-pyrrolo[2,3-c]pyridin-7(6H)-ones (A) and the 1-methyl-1H-pyrrolo[2,3-c]pyridin-7(6H)-ones (B). Each series demonstrated excellent activity in binding and cellular assays, and lead compounds from each series demonstrated significant efficacy in in vivo tumor xenograft models.
April 11, 2018: Bioorganic & Medicinal Chemistry Letters
Van Minh Dang, Stephen Joseph, Huu Tap Van, Thi Lan Anh Mai, Thi Minh Hoa Duong, Simon Weldon, Paul Munroe, David Mitchell, Sarasadat Taherymoosavi
Heavy metal contamination of crop lands surrounding mines in North Vietnam is a major environmental issue for both farmers and the population as a whole. Technology for the production of biochar at a village and household level has been successfully introduced into Vietnamese villages. This study was undertaken to determine if rice straw biochar produced in simple drum ovens could remediate contaminated land. Tests were also carried out to determine if biochar and apatite mixed together could be more effective than biochar alone...
April 20, 2018: Environmental Technology
Nutsuchar Wangtiraumnuay, Waleed Abed Alnabi, Mai Tsukikawa, Avrey Thau, Jenina Capasso, Reuven Sharony, Chris F Inglehearn, Alex V Levin
BACKGROUND/AIMS: Pigmentary retinal dystrophy and macular dystrophy have been previously reported in Heimler syndrome due to mutations in PEX1. Here we reported the ocular manifestations in Heimler syndrome due to mutations in PEX6. MATERIALS AND METHODS: Medical records were reviewed to identify patient demographics, ophthalmic and systemic findings, and results of diagnostic testing including whole genome sequencing. RESULTS: Patient 1 is 12-year-old boy with a novel mutation c...
June 2018: Ophthalmic Genetics
Francesco Fiorentino, Antonello Mai, Dante Rotili
Lysine acetylation is a post-translational modification of both histone and nonhistone proteins that is catalyzed by lysine acetyltransferases and plays a key role in numerous biological contexts. The dysregulation of this enzyme activity is implicated in many human pathologies such as cancer, neurological and inflammatory disorders. Many lysine acetyltransferase inhibitors (KATi) have been developed so far, but there is still the need for new, more potent, metabolically stable and selective KATi as chemical tools for studying KAT biology and/or as potential therapeutic agents...
April 20, 2018: Future Medicinal Chemistry
Lin-Quan Tang, Tong-Yu Lu, Yang Li, Shao-Yan Guo, Qi-Yu Zhong, Meng-Sha Zou, Bo-Lin Chen, Qing-Nan Tang, Wen-Hui Chen, Shan-Shan Guo, Li-Ting Liu, Qiu-Yan Chen, Ling Guo, Hao-Yuan Mo, Rui Sun, Dong-Hua Luo, Chong Zhao, Chao-Nan Qian, Xiang Guo, Mu-Sheng Zeng, Hai-Qiang Mai
Objectives: To evaluate the patterns of failure and survival trends of patients with stage I nasopharyngeal carcinoma (NPC) treated with radiotherapy alone over the last 20 years. Materials and Methods: A retrospective cohort study was conducted on 720 patients with stage I NPC who were treated with curative two-dimensional radiotherapy (2DRT), three-dimensional conformal radiotherapy (3DRT), or intensity-modulated radiotherapy (IMRT) between January 1990 and December 2012. The patients were categorized into four calendar periods (1990-1996, 1997-2002, 2003-2007, and 2008-2012) and four age subgroups (18-39, 40-49, 50-59, and >60)...
2018: Journal of Cancer
Megumi Shiota, Masayuki Naya, Takehiro Yamamoto, Takako Hishiki, Takeharu Tani, Hiroyuki Takahashi, Akiko Kubo, Daisuke Koike, Mai Itoh, Mitsuyo Ohmura, Yasuaki Kabe, Yuki Sugiura, Nobuyoshi Hiraoka, Takayuki Morikawa, Keiyo Takubo, Kentaro Suina, Hideaki Nagashima, Oltea Sampetrean, Osamu Nagano, Hideyuki Saya, Shogo Yamazoe, Hiroyuki Watanabe, Makoto Suematsu
Gold deposition with diagonal angle towards boehmite-based nanostructure creates random arrays of horse-bean-shaped nanostructures named gold-nanofève (GNF). GNF generates many electromagnetic hotspots as surface-enhanced Raman spectroscopy (SERS) excitation sources, and enables large-area visualization of molecular vibration fingerprints of metabolites in human cancer xenografts in livers of immunodeficient mice with sufficient sensitivity and uniformity. Differential screening of GNF-SERS signals in tumours and those in parenchyma demarcated tumour boundaries in liver tissues...
April 19, 2018: Nature Communications
Laetitia Minh Maï Le, Balázs Kégl, Alexandre Gramfort, Camille Marini, David Nguyen, Mehdi Cherti, Sana Tfaili, Ali Tfayli, Arlette Baillet-Guffroy, Patrice Prognon, Pierre Chaminade, Eric Caudron
The use of monoclonal antibodies (mAbs) constitutes one of the most important strategies to treat patients suffering from cancers such as hematological malignancies and solid tumors. These antibodies are prescribed by the physician and prepared by hospital pharmacists. An analytical control enables the quality of the preparations to be ensured. The aim of this study was to explore the development of a rapid analytical method for quality control. The method used four mAbs (Infliximab, Bevacizumab, Rituximab and Ramucirumab) at various concentrations and was based on recording Raman data and coupling them to a traditional chemometric and machine learning approach for data analysis...
July 1, 2018: Talanta
Do Thi Mai, David C Stuckey, Seungdae Oh
This study investigated the effects and fate of CIP on anaerobic sludge over a wide range of concentrations (0.05-50 mg/L), and 0.5-50 mg/L significantly inhibited organic removal and methanogenic activity, increased volatile fatty acids accumulation and low molecular weight soluble microbial products (SMPs), including p-cresol and nitrogen-containing compounds. Although microbial communities exposed to CIP did not differ significantly from the control in species diversity indices, Syntrophobacter and Methanothrix associated with acetogenesis and acetoclastic methanogenesis, respectively, were underrepresented in the CIP-exposed communities...
April 4, 2018: Bioresource Technology
Huynh Nhu Mai, Yoon Hee Chung, Eun-Joo Shin, Naveen Sharma, Ji Hoon Jeong, Choon-Gon Jang, Kuniaki Saito, Toshitaka Nabeshima, Dora Reglodi, Hyoung-Chun Kim
IL-6 has been recognized as an anticonvulsant against certain neuroexcitotoxicities. We aimed to investigate on the interactive role between IL-6 and PACAP in cocaine-induced kindling behaviors. Although we found that cocaine (45 mg/kg, i.p./day x 5) significantly increased IL-6 and TNF-α expression, it resulted in a decrease in IFN-γ expression. We observed that the cocaine-induced increase in IL-6 expression was more pronounced than that in TNF-α expression. Genetic depletion of IL-6 significantly activated cocaine kindling behaviors...
April 16, 2018: Food and Chemical Toxicology
Mai Shimizu, Kentaro Takenaka, Takeo Suzuki, Aya Miyasaka, Taiki Matsuda, Tatsuhiko Iwase, Hitoshi Kyotani
A ready-made dry medium method for Staphylococcus aureus count, the Medi·Ca SA method incubated at 35 or 37°C, was compared with the Baird-Parker method (AOAC Official Method SM 975.55 ) for 11 food matrices: raw beef, raw ground beef, raw lamb, cooked ham, raw salmon, frozen prawn, fresh chilled pasta, pasteurized milk, natural cheese, cream puff, and potato salad. The mean difference between the two methods at each contamination level for each matrix was <0.5 log10 , and the 95% confidence intervals on the mean differences fell within the range of -0...
April 19, 2018: Journal of AOAC International
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