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https://www.readbyqxmd.com/read/28315925/fine-mapping-of-the-restorer-gene-rfp3-from-an-iranian-primitive-rye-secale-cereale-l
#1
Bernd Hackauf, Eva Bauer, Viktor Korzun, Thomas Miedaner
A comparative genetics approach allowed to precisely determine the map position of the restorer gene Rfp3 in rye and revealed that Rfp3 and the restorer gene Rfm1 in barley reside at different positions in a syntenic 4RL/6HS segment. Cytoplasmic male sterility (CMS) is a reliable and striking genetic mechanism for hybrid seed production. Breeding of CMS-based hybrids in cereals requires the use of effective restorer genes as an indispensable pre-requisite. We report on the fine mapping of a restorer gene for the Pampa cytoplasm in winter rye that has been tapped from the Iranian primitive rye population Altevogt 14160...
March 18, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28279523/phytotoxic-phenylpropanoids-isolated-from-ophryosporus-charua-griseb-hieron
#2
Margarita M Del M Bravetti, Raquel V Vico, Maria C Carpinella, Carlos C Ferrayoli, Sara M Palacios
Bioguided isolation of the EtOH extract from the medicinal native plant, Ophryosporus charua, against Raphanus sativus, yielded three phenylpropanoids, charuol A [(Z)-4-((1S,2R)-3-acetoxy-1,2-dihydroxypropyl)phenyl) 2-methylbut-2-enoate], charuepoxide [(Z)-4-((2S,3R)-3-(acetoxymethyl oxiran-2-yl)phenyl) 2-methylbut-2-enoate] and charuol B [(Z)-4-((1R,2R)-3-acetoxy-1,2-dihydroxypropyl)phenyl) 2-methylbut-2-enoate]. Their structures and absolute configuration were established by extensive spectroscopic analyses...
March 6, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28278512/targeted-segment-transfer-from-rye-chromosome-2r-to-wheat-chromosomes-2a-2b-and-7b
#3
Tianheng Ren, Zhi Li, Benju Yan, Feiquan Tan, Zongxiang Tang, Shulan Fu, Manyu Yang, Zhenglong Ren
Increased chromosome instability was induced by a rye (Secale cereale L.) monosomic 2R chromosome into wheat (Triticum aestivum L.). Centromere breakage and telomere dysfunction result in high rates of chromosome aberrations, including breakages, fissions, fusions, deletions, and translocations. Plants with target traits were sequentially selected to produce a breeding population, from which 3 translocation lines with target traits have been selected. In these lines, wheat chromosomes 2A, 2B, and 7B recombined with segments of the rye chromosome arm 2RL...
March 10, 2017: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/28258493/introduction-of-thinopyrum-intermedium-ssp-trichophorum-chromosomes-to-wheat-by-trigeneric-hybridization-involving-triticum-secale-and-thinopyrum-genera
#4
Jianbo Li, Tao Lang, Bin Li, Zhihui Yu, Hongjin Wang, Guangrong Li, Ennian Yang, Zujun Yang
Fluorescence in situ hybridization and molecular markers have confirmed that several chromosomes from Thinopyrum intermedium ssp. trichophorum have been added to a wheat background, which originated from a cross between a wheat- Thinopyrum partial amphiploid and triticale. The lines displayed blue grains and resistance to wheat stripe rust. Thinopyrum intermedium has been used as a valuable resource for improving the disease resistance and yield potential of wheat. With the aim to transfer novel genetic variation from Th...
March 3, 2017: Planta
https://www.readbyqxmd.com/read/28248976/residual-soil-nitrate-content-and-profitability-of-five-cropping-systems-in-northwest-iowa
#5
Robert L De Haan, Matthew A Schuiteman, Ronald J Vos
Many communities in the Midwestern United States obtain their drinking water from shallow alluvial wells that are vulnerable to contamination by NO3-N from the surrounding agricultural landscape. The objective of this research was to assess cropping systems with the potential to produce a reasonable return for farmers while simultaneously reducing the risk of NO3-N movement into these shallow aquifers. From 2009 to 2013 we conducted a field experiment in northwest Iowa in which we evaluated five cropping systems for residual (late fall) soil NO3-N content and profitability...
2017: PloS One
https://www.readbyqxmd.com/read/28150529/free-asparagine-and-sugars-profile-of-cereal-species-the-potential-of-cereals-for-acrylamide-formation-in-foods
#6
Slađana Žilić, Dejan Dodig, Zorica Basić, Jelena Vančetović, Primož Titan, Nenad Đurić, Nataša Tolimir
Cereals based food is one of the major source of Maillard reaction products in the diet. Free amino acids and reducing sugars are considered to be the main precursors in the formation of these heat-induced compounds. In order to determine genetic resources with reduced potential for acrylamide formation, the content of sugars, as well as free asparagine were analyzed in a total of 30 cultivars of 10 varieties belonging to eight species (Triticum aestivum var. lutescens, T. aestivum var. alba, T. aestivum var...
February 2, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28149679/the-dna-methylation-level-against-the-background-of-the-genome-size-and-t-heterochromatin-content-in-some-species-of-the-genus-secale-l
#7
Anna Kalinka, Magdalena Achrem, Paulina Poter
Methylation of cytosine in DNA is one of the most important epigenetic modifications in eukaryotes and plays a crucial role in the regulation of gene activity and the maintenance of genomic integrity. DNA methylation and other epigenetic mechanisms affect the development, differentiation or the response of plants to biotic and abiotic stress. This study compared the level of methylation of cytosines on a global (ELISA) and genomic scale (MSAP) between the species of the genus Secale. We analyzed whether the interspecific variation of cytosine methylation was associated with the size of the genome (C-value) and the content of telomeric heterochromatin...
2017: PeerJ
https://www.readbyqxmd.com/read/28145819/xiv-congreso-nacional-de-secal-las-palmas-de-gran-canaria-islas-canarias-espa%C3%A3-a-junio-2017
#8
José Luis Martín-Barrasa
No abstract text is available yet for this article.
February 2017: Laboratory Animals
https://www.readbyqxmd.com/read/27888547/towards-a-whole-genome-sequence-for-rye-secale-cereale-l
#9
Eva Bauer, Thomas Schmutzer, Ivan Barilar, Martin Mascher, Heidrun Gundlach, Mihaela M Martis, Sven O Twardziok, Bernd Hackauf, Andres Gordillo, Peer Wilde, Malthe Schmidt, Viktor Korzun, Klaus F X Mayer, Karl Schmid, Chris-Carolin Schön, Uwe Scholz
We report on a whole-genome draft sequence of rye (Secale cereale L.). Rye is a diploid Triticeae species closely related to wheat and barley, and an important crop for food and feed in Central and Eastern Europe. Through whole-genome shotgun sequencing of the 7.9-Gbp genome of the winter rye inbred line Lo7 we obtained a de novo assembly represented by 1.29 million scaffolds covering a total length of 2.8 Gbp. Our reference sequence represents nearly the entire low-copy portion of the rye genome. This genome assembly was used to predict 27 784 rye gene models based on homology to sequenced grass genomes...
March 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/27842961/contrasting-effects-of-biochar-on-n2o-emission-and-n-uptake-at-different-n-fertilizer-levels-on-a-temperate-sandy-loam
#10
Zhencai Sun, Anja Sänger, Philip Rebensburg, Peter Lentzsch, Stephan Wirth, Martin Kaupenjohann, Andreas Meyer-Aurich
Biochar has been frequently suggested as an amendment to improve soil quality and mitigate climate change. To investigate the optimal management of nitrogen (N) fertilization, we examined the combined effect of biochar and N fertilizer on plant N uptake and N2O emissions in a cereal rotation system in a randomized two-factorial field experiment on a sandy loam soil in Brandenburg, Germany. The biochar treatment received 10Mgha(-1) wood-derived biochar in September 2012. Four levels of N fertilizer, corresponding to 0, 50%, 100%, 130% of the recommended fertilizer level, were applied in winter wheat (Triticum aestivum L...
February 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/27833625/dart-markers-effectively-target-gene-space-in-the-rye-genome
#11
Piotr Gawroński, Magdalena Pawełkowicz, Katarzyna Tofil, Grzegorz Uszyński, Saida Sharifova, Shivaksh Ahluwalia, Mirosław Tyrka, Maria Wędzony, Andrzej Kilian, Hanna Bolibok-Brągoszewska
Large genome size and complexity hamper considerably the genomics research in relevant species. Rye (Secale cereale L.) has one of the largest genomes among cereal crops and repetitive sequences account for over 90% of its length. Diversity Arrays Technology is a high-throughput genotyping method, in which a preferential sampling of gene-rich regions is achieved through the use of methylation sensitive restriction enzymes. We obtained sequences of 6,177 rye DArT markers and following a redundancy analysis assembled them into 3,737 non-redundant sequences, which were then used in homology searches against five Pooideae sequence sets...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27818381/rye-secale-cereale-supernumerary-b-chromosomes-associated-with-heat-tolerance-during-early-stages-of-male-sporogenesis
#12
H Sofia Pereira, Margarida Delgado, Wanda Viegas, João M Rato, Augusta Barão, Ana D Caperta
BACKGROUND AND AIMS: Rye supernumerary (B) chromosomes have an accumulation mechanism involving the B subtelomeric domain highly enriched in D1100- and E3900-related sequences. In this work, the effects of heat stress during the early stages of male meiosis in 0B and +B plants were studied. METHODS: In-depth cytological analyses of chromatin structure and behaviour were performed on staged rye meiocytes utilizing DAPI, fluorescence in situ hybridization and 5-methylcytosine immune labelling...
February 2017: Annals of Botany
https://www.readbyqxmd.com/read/27743251/similarities-and-differences-in-the-nuclear-genome-organization-within-pooideae-species-revealed-by-comparative-genomic-in-situ-hybridization-gish
#13
Joanna Majka, Maciej Majka, Michał Kwiatek, Halina Wiśniewska
In this paper, we highlight the affinity between the genomes of key representatives of the Pooideae subfamily, revealed at the chromosomal level by genomic in situ hybridization (GISH). The analyses were conducted using labeled probes from each species to hybridize with chromosomes of every species used in this study based on a "round robin" rule. As a result, the whole chromosomes or chromosome regions were distinguished or variable types of signals were visualized to prove the different levels of the relationships between genomes used in this study...
October 14, 2016: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27700105/screening-for-ricinoleic-acid-as-a-chemical-marker-for-secale-cornutum-in-rye-by-high-performance-thin-layer-chromatography-with-fluorescence-detection
#14
Claudia Oellig
Ricinoleic acid as the characteristic fatty acid of Secale cornutum oil is a good marker for Secale cornutum impurities in cereal. The presented screening for ricinoleic acid in rye by high-performance thin-layer chromatography with fluorescence detection (HPTLC-FLD) offers a selective and sensitive method for the determination of Secale cornutum and is very different from existing gas chromatographic analyses. Lipid extraction was followed by transesterification and solid-phase extraction cleanup; thereafter, extracts were selectively derivatized with 2-naphthoyl chloride and analyzed by HPTLC-FLD with silica gel plates and cyclohexane/diisopropyl ether/formic acid (86:14:1, v/v/v) as mobile phase...
November 2, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27668423/molecular-cytogenetic-characterization-of-new-wheat-rye-1r-1b-substitution-and-translocation-lines-from-a-chinese-secale-cereal-l-aigan-with-resistance-to-stripe-rust
#15
Zhi Li, Zhenglong Ren, Feiquan Tan, Zongxiang Tang, Shulan Fu, Benju Yan, Tianheng Ren
Secale cereale L. has been used worldwide as a source of genes for agronomic and resistance improvement. In this study, a stable wheat-rye substitution line and 3 primary 1RS.1BL translocation lines were selected from the progeny of the crossing of the Chinese local rye Aigan variety and wheat cultivar Mianyang11. The substitution and translocation lines were identified by molecular cytogenetic analysis. PCR results, fluorescence in situ hybridization and acid polyacrylamide gel electrophoresis indicated that there were a pair of 1R chromosomes in the substitution line which have been named RS1200-3, and a pair of 1RS...
2016: PloS One
https://www.readbyqxmd.com/read/27659326/characterization-and-expression-analyses-of-the-h%C3%A2-%C2%BA-pyrophosphatase-gene-in-rye
#16
Chang-Shui Wang, Qian-Tao Jiang, Jian Ma, Xiu-Ying Wang, Ji-Rui Wang, Guo-Yue Chen, Peng-Fei Qi, Yuan-Ying Peng, Xiu-Jin Lan, You-Liang Zheng, Yu-Ming Wei
The H⁺-pyrophosphatase (H⁺-PPase) gene plays an important role in maintaining intracellular proton gradients. Here, we characterized the full-length complementary DNA (cDNA) and DNA of the H⁺-PPase gene ScHP1 in rye (Secale cereale L. 'Qinling'). We determined the subcellular localization of this gene and predicted the corresponding protein structure. We analysed the evolutionary relationship between ScHP1 and H⁺-PPase genes in other species, and did real-time quantitative polymerase chain reaction to explore the expression patterns of ScHP1 in rye plants subjected to N, P and K deprivation and to cold, high-salt and drought stresses...
September 2016: Journal of Genetics
https://www.readbyqxmd.com/read/27630658/genetic-distinctiveness-of-rye-in-situ-accessions-from-portugal-unveils-a-new-hotspot-of-unexplored-genetic-resources
#17
Filipa Monteiro, Patrícia Vidigal, André B Barros, Ana Monteiro, Hugo R Oliveira, Wanda Viegas
Rye (Secale cereale L.) is a cereal crop of major importance in many parts of Europe and rye breeders are presently very concerned with the restrict pool of rye genetic resources available. Such narrowing of rye genetic diversity results from the presence of "Petkus" pool in most modern rye varieties as well as "Petkus" × "Carsten" heterotic pool in hybrid rye breeding programs. Previous studies on rye's genetic diversity revealed moreover a common genetic background on landraces (ex situ) and cultivars, regardless of breeding level or geographical origin...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27496892/secal-en-el-congreso-de-felasa-bruselas
#18
Juan Rodríguez Cuesta
No abstract text is available yet for this article.
August 2016: Laboratory Animals
https://www.readbyqxmd.com/read/27468091/rye-b-chromosomes-encode-a-functional-argonaute-like-protein-with-in%C3%A2-vitro-slicer-activities-similar-to-its-a-chromosome-paralog
#19
Wei Ma, Tobias Sebastian Gabriel, Mihaela Maria Martis, Torsten Gursinsky, Veit Schubert, Jan Vrána, Jaroslav Doležel, Heidrun Grundlach, Lothar Altschmied, Uwe Scholz, Axel Himmelbach, Sven-Erik Behrens, Ali Mohammad Banaei-Moghaddam, Andreas Houben
B chromosomes (Bs) are supernumerary, dispensable parts of the nuclear genome, which appear in many different species of eukaryote. So far, Bs have been considered to be genetically inert elements without any functional genes. Our comparative transcriptome analysis and the detection of active RNA polymerase II (RNAPII) in the proximity of B chromatin demonstrate that the Bs of rye (Secale cereale) contribute to the transcriptome. In total, 1954 and 1218 B-derived transcripts with an open reading frame were expressed in generative and vegetative tissues, respectively...
January 2017: New Phytologist
https://www.readbyqxmd.com/read/27465692/scbx-gene-based-association-analysis-of-hydroxamate-content-in-rye-secale-cereale-l
#20
Monika Rakoczy-Trojanowska, Wacław Orczyk, Paweł Krajewski, Jan Bocianowski, Anna Stochmal, Mariusz Kowalczyk
Hydroxamates (HX) are major secondary metabolites synthesized by rye and are responsible for some of the unique properties of this cereal, including good tolerance of biotic and abiotic stresses and allelopathy. Recently, five genes encoding enzymes taking part in HX biosynthesis have been sequenced and characterized, which was the starting point to undertake the present study. Association analysis of the content of six HX-HBOA (2-hydroxy-1,4-benzoxazin-3-one), GDIBOA (2,4-dihydroxy-1,4-benzoxazin-3(4H)-one glucoside), DIBOA (2,4-dihydroxy-1,4-benzoxazin-3(4H)-one), GDIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3(4H)-one glucoside), DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3(4H)-one) and MBOA (6-methoxy-benzoxazolin-2(3H)-one) in the above-ground parts of plants and roots was performed on a population consisting of 102 and 121 diverse inbred lines, in 2013 and 2014, respectively...
February 2017: Journal of Applied Genetics
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